Abstract In the early 1950's,'host-controlled variation in bacterial viruses' was reported as a
non-hereditary phenomenon: one cycle of viral growth on certain bacterial hosts affected the …

We describe a simple system for reversible, stable integration of plasmid-borne genes into
the Escherichia coli chromosome. Most ordinary E. coli strains and a variety of pBR322 …

Chromosomal editing constitutes the direct and specific modification of the genetic
information present in the chromosome. In the bacterium Escherichia coli, strategies were …

The bacterial AAA+ enhancer-binding proteins (EBPs) HrpR and HrpS (HrpRS) of
Pseudomonas syringae (Ps) activate σ54-dependent transcription at the hrpL promoter; …

Many of the important changes in evolution are regulatory in nature. Sequenced bacterial
genomes point to flexibility in regulatory circuits but we do not know how regulation is …

This work describes the novel use of tolC as a selectable/counter-selectable marker for the
facile modification of DNA in Escherichia coli. Expression of TolC (an outer membrane …

The small, stable RNA molecule encoded by ssrA, known as tmRNA or 10Sa RNA, is
required for the growth of certain hybrid λ imm P22 phages in Escherichia coli. tmRNA has …

Mutations beneficial in one environment may cause costs in different environments, resulting
in antagonistic pleiotropy. Here, we describe a novel form of antagonistic pleiotropy that …

Regulation of gene transcription by the progesterone receptor (PR) in cooperation with
coactivator/corepressor complexes coordinates crucial processes in female reproduction. To …

Plasmid-based cloning and expression of genes in Escherichia coli can have several
problems: plasmid destabilization; toxicity of gene products; inability to achieve complete …