 |
REBASE Methylation Sensitivity 11/5/2024 |
 |
Click on the  below to see the enzyme's supplier listings. |
| Methylation Sensitivity Data 5-methylcytosine | |
|
AatII |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 717 |
m5
G A C G T C
C T G C A G
m5
|
| 3975 |
m5 m5
G A C G T C
C T G C A G
m5 m5
|
|
AccII |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1659 |
m5
C G C G
G C G C
|
| 567 |
m5
C G C G
G C G C
m5
|
| 1660 |
m5
C G C G
G C G C
m5
|
| 1661 |
m5 m5
C G C G
G C G C
|
| 1662 |
m5
C G C G
G C G C
|
| 1663 |
m5
C G C G
G C G C
m5
|
|
AccIII |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 693 |
m5
T C C G G A
A G G C C T
m5
|
|
AciI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1768 |
C C G C
G G C G
m5
|
| 3978 |
m5m5 m5
C C G C
G G C G
m5
|
| 1769 |
m5
C C G C
G G C G
|
| 917 |
m5
C C G C
G G C G
m5
|
|
AclI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 991 |
m5
A A C G T T
T T G C A A
m5
|
|
AfeI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1872 |
m5
A G C G C T
T C G C G A
m5
|
| 3981 |
m5 m5
A G C G C T
T C G C G A
m5 m5
|
|
AgeI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 3982 |
m5m5
A C C G G T
T G G C C A
m5m5
|
| 856 |
m5
A C C G G T
T G G C C A
m5
|
|
AhaII | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 569 |
m5
G R C G Y C
C Y G C R G
m5
|
|
AjiI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2554 |
m5
C A C G T C
G T G C A G
m5
|
|
Aor51HI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1599 |
m5
A G C G C T
T C G C G A
m5
|
|
AosII | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1401 |
m5
G R C G Y C
C Y G C R G
m5
|
|
ApeI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1523 |
m5
A C G C G T
T G C G C A
m5
|
|
AscI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1732 |
m5
G G C G C G C C
C C G C G C G G
|
| 1881 |
m5
G G C G C G C C
C C G C G C G G
m5
|
| 1882 |
m5
G G C G C G C C
C C G C G C G G
m5 m5
|
| 1733 |
m5
G G C G C G C C
C C G C G C G G
m5
|
| 992 |
m5 m5
G G C G C G C C
C C G C G C G G
m5 m5
|
| 3988 |
m5 m5 m5m5
G G C G C G C C
C C G C G C G G
m5m5 m5 m5
|
| 1730 |
m5
G G C G C G C C
C C G C G C G G
|
| 1731 |
m5
G G C G C G C C
C C G C G C G G
m5
|
| 1883 |
m5
G G C G C G C C
C C G C G C G G
m5
|
| 1884 |
m5
G G C G C G C C
C C G C G C G G
m5 m5
|
| 1736 |
m5
G G C G C G C C
C C G C G C G G
m5
|
|
AsiSI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2193 |
m5
G C G A T C G C
C G C T A G C G
m5
|
| 2208 |
m5
G C G A T C G C
C G C T A G C G
m5 m5
|
| 2201 |
m5 m5
G C G A T C G C
C G C T A G C G
m6
|
| 2196 |
m5 m5
G C G A T C G C
C G C T A G C G
m5 m5
|
| 2199 |
m5 m5
G C G A T C G C
C G C T A G C G
m5 m5 m5
|
| 2200 |
m5 m5 m5
G C G A T C G C
C G C T A G C G
m5 m5 m5
|
| 2197 |
m5 m5
G C G A T C G C
C G C T A G C G
m5 m5
|
| 2209 |
m5
G C G A T C G C
C G C T A G C G
m5 m5
|
| 2198 |
m5
G C G A T C G C
C G C T A G C G
m5 m5
|
|
AspCNI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2027 |
m5
G C C G C
C G G C G
m5
|
| 2032 |
m5
G C C G C
C G G C G
|
| 1931 |
m5
G C C G C
C G G C G
m5
|
|
AsuII |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2405 |
m5
T T C G A A
A A G C T T
m5
|
|
AvaI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 3989 |
m5m5m5
C Y C G R G
G R G C Y C
m5m5m5
|
| 339 |
m5 m5
C Y C G R G
G R G C Y C
|
| 627 |
m5
C Y C G R G
G R G C Y C
m5
|
|
AviII | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1529 |
m5
T G C G C A
A C G C G T
m5
|
|
BauI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2555 |
m5
C A C G A G
G T G C T C
m5
|
|
BbeI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 648 |
m5
G G C G C C
C C G C G G
m5
|
|
BbiII | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 832 |
m5
G R C G Y C
C Y G C R G
m5
|
|
BbrPI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 583 |
m5 m5
C A C G T G
G T G C A C
m5 m5
|
| 776 |
m5
C A C G T G
G T G C A C
m5
|
|
Bca77I | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 888 |
m5
W C C G G W
W G G C C W
m5
|
|
BceAI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1866 |
A C G G C
T G C C G
m5
|
| 1912 |
m5
A C G G C
T G C C G
m5
|
| 1987 |
m5
A C G G C
T G C C G
m5
|
| 3998 |
m5 m5
A C G G C
T G C C G
m5m5
|
|
BcgI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2118 |
C G A N N N N N N T G C
G C T N N N N N N A C G
m5
|
| 2454 |
m5
C G A N N N N N N T G C
G C T N N N N N N A C G
m5
|
| 1879 |
m5 m5
C G A N N N N N N T G C
G C T N N N N N N A C G
m5
|
| 3999 |
m5 m5
C G A N N N N N N T G C
G C T N N N N N N A C G
m5 m5
|
|
BepI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 656 |
m5
C G C G
G C G C
m5
|
|
BloMGI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2779 |
m5 m5
C C G C G G
G G C G C C
m5 m5
|
| 2778 |
m5
C C G C G G
G G C G C C
m5
|
|
BmeT110I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2474 |
m5
C Y C G R G
G R G C Y C
m5
|
|
BmgBI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4005 |
m5 m5 m5
C A C G T C
G T G C A G
m5
|
| 2155 |
m5
C A C G T C
G T G C A G
m5
|
|
BsaAI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4011 |
m5 m5
Y A C G T R
R T G C A Y
m5 m5
|
| 780 |
m5
Y A C G T R
R T G C A Y
m5
|
|
BsaHI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 782 |
m5
G R C G Y C
C Y G C R G
m5
|
| 4012 |
m5 m5m5
G R C G Y C
C Y G C R G
m5m5 m5
|
|
BsaWI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4015 |
m5m5
W C C G G W
W G G C C W
m5m5
|
| 997 |
m5
W C C G G W
W G G C C W
m5
|
|
Bse634I | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2217 |
m5
R C C G G Y
Y G G C C R
m5
|
|
BseAI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2422 |
m5
T C C G G A
A G G C C T
m5
|
|
BseCI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2399 |
m5
A T C G A T
T A G C T A
m5
|
|
Bsh1236I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1664 |
m5
C G C G
G C G C
|
| 1665 |
m5
C G C G
G C G C
m5
|
| 1666 |
m5
C G C G
G C G C
m5
|
| 1667 |
m5 m5
C G C G
G C G C
|
| 1276 |
m5 m5
C G C G
G C G C
m5 m5
|
| 1668 |
m5
C G C G
G C G C
|
| 1669 |
m5
C G C G
G C G C
m5
|
|
Bsh1285I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1741 |
m5 m5
C G R Y C G
G C Y R G C
m5 m5
|
|
BshTI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1279 |
m5
A C C G G T
T G G C C A
m5
|
|
BsiEI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2016 |
m5
C G R Y C G
G C Y R G C
|
| 2018 |
m5
C G R Y C G
G C Y R G C
m5
|
| 4020 |
m5 m5m5
C G R Y C G
G C Y R G C
m5m5 m5
|
| 857 |
m5 m5
C G R Y C G
G C Y R G C
m5 m5
|
| 2017 |
m5
C G R Y C G
G C Y R G C
|
|
BsiSI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2414 |
m5
C C G G
G G C C
m5
|
|
BsiWI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2112 |
m5
C G T A C G
G C A T G C
|
| 2111 |
m5
C G T A C G
G C A T G C
m5
|
| 2114 |
m5 m6
C G T A C G
G C A T G C
|
| 858 |
m5 m5
C G T A C G
G C A T G C
m5 m5
|
|
BsmBI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2088 |
C G T C T C
G C A G A G
m5
|
| 1880 |
m5
C G T C T C
G C A G A G
m5
|
| 4024 |
m5 m5 m5
C G T C T C
G C A G A G
m5
|
| 2106 |
m5
C G T C T C
G C A G A G
m5
|
|
BsoBI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4027 |
m5m5m5
C Y C G R G
G R G C Y C
m5m5m5
|
| 996 |
m5
C Y C G R G
G R G C Y C
m5
|
|
Bsp68I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1282 |
m5 m5
T C G C G A
A G C G C T
m5 m5
|
|
Bsp119I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1036 |
m5
T T C G A A
A A G C T T
m5
|
|
Bsp143II | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1040 |
m5
R G C G C Y
Y C G C G R
m5
|
|
BspDI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 859 |
m5
A T C G A T
T A G C T A
m5
|
|
BspEI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4030 |
m5m5
T C C G G A
A G G C C T
m5m5
|
| 686 |
m5
T C C G G A
A G G C C T
m5
|
|
BspFNI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 3428 |
m5
C G C G
G C G C
m5
|
| 3429 |
m5
C G C G
G C G C
|
|
BspMII | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 689 |
m5
T C C G G A
A G G C C T
m5
|
|
BspT104I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1606 |
m5
T T C G A A
A A G C T T
m5
|
|
BsrBI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4034 |
m5m5 m5 m5
C C G C T C
G G C G A G
m5
|
| 2097 |
m5
C C G C T C
G G C G A G
|
| 834 |
m5
C C G C T C
G G C G A G
m5
|
| 833 |
m5 m5
C C G C T C
G G C G A G
m5
|
| 2099 |
m5
C C G C T C
G G C G A G
m6
|
|
BsrFI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4036 |
m5m5 m5
R C C G G Y
Y G G C C R
m5 m5m5
|
| 860 |
m5
R C C G G Y
Y G G C C R
m5
|
|
BssAI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2401 |
m5
R C C G G Y
Y G G C C R
m5
|
|
BssHII |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1750 |
m5
G C G C G C
C G C G C G
|
| 3516 |
m5
G C G C G C
C G C G C G
|
| 1885 |
m5
G C G C G C
C G C G C G
m5
|
| 1534 |
m5
G C G C G C
C G C G C G
m5
|
| 1753 |
m5
G C G C G C
C G C G C G
m5
|
| 4039 |
m5 m5 m5
G C G C G C
C G C G C G
m5 m5 m5
|
| 1749 |
m5
G C G C G C
C G C G C G
|
| 1752 |
m5
G C G C G C
C G C G C G
m5
|
| 1886 |
m5
G C G C G C
C G C G C G
m5 m5
|
| 1754 |
m5
G C G C G C
C G C G C G
m5
|
|
BssSI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4040 |
m5 m5
C A C G A G
G T G C T C
m5 m5
|
| 993 |
m5
C A C G A G
G T G C T C
m5
|
|
BstBI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 790 |
m5
T T C G A A
A A G C T T
m5
|
|
BstUI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1232 |
m5
C G C G
G C G C
|
| 1236 |
m5
C G C G
G C G C
m5
|
| 1234 |
m5
C G C G
G C G C
m5
|
| 1237 |
m5 m5
C G C G
G C G C
|
| 2773 |
m5 m5
C G C G
G C G C
m5 m5
|
| 1233 |
m5
C G C G
G C G C
|
| 1235 |
m5
C G C G
G C G C
m5
|
|
Bsu15I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1045 |
m5
A T C G A T
T A G C T A
m5
|
|
BsuEII | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 869 |
m5
C G C G
G C G C
m5
|
|
BsuMI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 714 |
m5
C T C G A G
G A G C T C
m5
|
|
BtgZI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2227 |
m5
G C G A T G
C G C T A C
m5
|
| 2497 |
m5
G C G A T G
C G C T A C
m5 m5
|
| 4048 |
m5
G C G A T G
C G C T A C
m5 m5 m5
|
|
BtrI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2078 |
m5
C A C G T C
G T G C A G
m5
|
| 2080 |
m6
C A C G T C
G T G C A G
m5
|
| 1929 |
m5
C A C G T C
G T G C A G
m5
|
|
CboI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1325 |
m5
C C G G
G G C C
m5
|
|
CceI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1429 |
m5
C C G G
G G C C
m5
|
|
CfoI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1541 |
m5
G C G C
C G C G
m5
|
| 324 |
m5 m5
G C G C
C G C G
|
| 593 |
m5 m5
G C G C
C G C G
m5 m5
|
|
Cfr9I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 727 |
m5
C C C G G G
G G G C C C
|
| 733 |
m5
C C C G G G
G G G C C C
m5
|
|
Cfr10I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 794 |
m5
R C C G G Y
Y G G C C R
m5
|
|
Cfr42I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1287 |
m5 m5
C C G C G G
G G C G C C
m5 m5
|
|
ClaI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 302 |
m5
A T C G A T
T A G C T A
|
| 558 |
m5
A T C G A T
T A G C T A
m5
|
|
CpaAI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 3509 |
m5
C G C G
G C G C
m5
|
| 3510 |
m5 m5
C G C G
G C G C
m5 m5
|
| 3511 |
m5
C G C G
G C G C
m5
|
|
CpoI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1288 |
m5 m5
C G G W C C G
G C C W G G C
m5 m5
|
|
CseI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2556 |
m5
G A C G C
C T G C G
m5
|
|
CspI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1543 |
m5
C G G W C C G
G C C W G G C
m5
|
|
CspAI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2416 |
m5
A C C G G T
T G G C C A
m5
|
|
Csp68KII | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 889 |
m5
T T C G A A
A A G C T T
m5
|
|
CviSIII | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1317 |
m5
T C G A
A G C T
m5
|
|
EagI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2005 |
m5
C G G C C G
G C C G G C
m5
|
| 926 |
m5 m5
C G G C C G
G C C G G C
m5 m5
|
| 4057 |
m5 m5m5
C G G C C G
G C C G G C
m5m5 m5
|
| 1998 |
m5
C G G C C G
G C C G G C
m5
|
| 2003 |
m5
C G G C C G
G C C G G C
|
|
EciI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1713 |
G G C G G A
C C G C C T
m5
|
| 1708 |
m5
G G C G G A
C C G C C T
|
| 1709 |
m5
G G C G G A
C C G C C T
m5
|
| 1902 |
m5
G G C G G A
C C G C C T
m5 m5
|
| 4058 |
m5
G G C G G A
C C G C C T
m5m5 m5m5
|
|
EclXI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1547 |
m5
C G G C C G
G C C G G C
m5
|
|
Eco47III |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 862 |
m5
A G C G C T
T C G C G A
m5
|
|
Eco52I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1289 |
m5 m5
C G G C C G
G C C G G C
m5 m5
|
|
Eco72I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1105 |
m5
C A C G T G
G T G C A C
m5
|
|
Eco88I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1106 |
m5
C Y C G R G
G R G C Y C
m5
|
|
Eco105I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1108 |
m5
T A C G T A
A T G C A T
m5
|
|
EheI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 831 |
m5
G G C G C C
C C G C G G
m5
|
|
EsaBC3I | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1259 |
m5
T C G A
A G C T
m5
|
|
Esp3I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 863 |
m5
C G T C T C
G C A G A G
m5
|
| 4063 |
m5 m5 m5
C G T C T C
G C A G A G
m5
|
|
FauI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 3434 |
m5
C C C G C
G G G C G
m5
|
| 4064 |
m5m5m5 m5
C C C G C
G G G C G
m5
|
| 995 |
m5
C C C G C
G G G C G
m5
|
|
FnuDII | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 566 |
m5
C G C G
G C G C
m5
|
| 896 |
m5
C G C G
G C G C
m5
|
|
FseI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4066 |
m5m5 m5m5
G G C C G G C C
C C G G C C G G
m5m5 m5m5
|
| 774 |
m5
G G C C G G C C
C C G G C C G G
m5
|
|
FspI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 799 |
m5
T G C G C A
A C G C G T
m5
|
| 4067 |
m5 m5
T G C G C A
A C G C G T
m5 m5
|
|
FspAI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2557 |
m5
R T G C G C A Y
Y A C G C G T R
m5
|
| 2215 |
m5 m5
R T G C G C A Y
Y A C G C G T R
m5 m5
|
|
HaeII |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 448 |
m5
R G C G C Y
Y C G C G R
m5
|
| 331 |
m5 m5
R G C G C Y
Y C G C G R
|
| 613 |
m5 m5
R G C G C Y
Y C G C G R
m5 m5
|
| 4068 |
m5 m5m5
R G C G C Y
Y C G C G R
m5m5 m5
|
|
HapII |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 505 |
m5
C C G G
G G C C
m5
|
|
HgaI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 946 |
G A C G C
C T G C G
m5
|
| 2156 |
m5
G A C G C
C T G C G
|
| 506 |
m5
G A C G C
C T G C G
m5
|
| 4069 |
m5 m5
G A C G C
C T G C G
m5 m5
|
|
HhaI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1960 |
m5
G C G C
C G C G
|
| 449 |
m5
G C G C
C G C G
m5
|
| 320 |
m5 m5
G C G C
C G C G
|
| 594 |
m5 m5
G C G C
C G C G
m5 m5
|
| 1961 |
m5
G C G C
C G C G
m5
|
|
Hin1I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1112 |
m5
G R C G Y C
C Y G C R G
m5
|
|
Hin6I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1114 |
m5
G C G C
C G C G
m5
|
|
HinP1I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1965 |
m5
G C G C
C G C G
|
| 802 |
m5
G C G C
C G C G
m5
|
| 4071 |
m5 m5
G C G C
C G C G
m5 m5
|
| 1966 |
m5
G C G C
C G C G
m5
|
|
HpaII |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 321 |
m5m5
C C G G
G G C C
|
| 598 |
m5m5
C C G G
G G C C
m5m5
|
| 2668 |
hm5
C C G G
G G C C
|
| 1483 |
m5
C C G G
G G C C
|
| 514 |
m5
C C G G
G G C C
m5
|
| 2672 |
h
C C G G
G G C C
m5
|
|
Hpy99I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2025 |
m5
C G W C G
G C W G C
|
| 2026 |
m5
C G W C G
G C W G C
m5
|
| 1797 |
m5 m5
C G W C G
G C W G C
m5 m5
|
|
HpyCH4IV |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1493 |
m5
A C G T
T G C A
m5
|
|
HpySE526I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 3372 |
m5
A C G T
T G C A
m5
|
|
HsoI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 873 |
m5
G C G C
C G C G
m5
|
|
KasI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 928 |
m5
G G C G C C
C C G C G G
m5
|
| 4078 |
m5 m5m5
G G C G C C
C C G C G G
m5m5 m5
|
|
Kpn2I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1119 |
m5
T C C G G A
A G G C C T
m5
|
|
KspI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1511 |
m5
C C G C G G
G G C G C C
m5
|
|
MaeII |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1187 |
m5
A C G T
T G C A
m5
|
|
MbiI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1291 |
m5
C C G C T C
G G C G A G
m5
|
|
MluI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2107 |
m5
A C G C G T
T G C G C A
m5
|
| 945 |
m5
A C G C G T
T G C G C A
m5
|
| 1238 |
m5 m5
A C G C G T
T G C G C A
m5 m5
|
| 3437 |
m5
A C G C G T
T G C G C A
|
| 3436 |
m5
A C G C G T
T G C G C A
m5
|
|
Mlu9273I | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1552 |
m5
T C G C G A
A G C G C T
m5
|
|
Mlu9273II | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1513 |
m5
G C C G G C
C G G C C G
m5
|
|
MroI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1556 |
m5
T C C G G A
A G G C C T
m5
|
|
MspI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 330 |
m5m5
C C G G
G G C C
|
| 599 |
m5m5
C C G G
G G C C
m5m5
|
| 2669 |
hm5
C C G G
G G C C
|
| 1484 |
m5
C C G G
G G C C
|
| 349 |
m5
C C G G
G G C C
m5
|
| 3398 |
m5
C C G G
G G C C
m5
|
| 2673 |
h
C C G G
G G C C
m5
|
| 3401 |
h
C C G G
G G C C
m5
|
|
MvnI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1559 |
m5
C G C G
G C G C
m5
|
|
NaeI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4088 |
m5m5 m5
G C C G G C
C G G C C G
m5 m5m5
|
| 806 |
m5
G C C G G C
C G G C C G
m5
|
|
NarI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 807 |
m5
G G C G C C
C C G C G G
m5
|
| 4089 |
m5 m5m5
G G C G C C
C C G C G G
m5m5 m5
|
|
NgoCI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 447 |
m5
R G C G C Y
Y C G C G R
m5
|
|
NgoMIV |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4092 |
m5m5 m5
G C C G G C
C G G C C G
m5 m5m5
|
| 809 |
m5
G C C G G C
C G G C C G
m5
|
|
NgoWI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 636 |
m5
R G C G C Y
Y C G C G R
m5
|
|
NmeAIII |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4095 |
m5m5
G C C G A G
C G G C T C
m5 m5 m5
|
| 2518 |
m5
G C C G A G
C G G C T C
m5
|
| 2519 |
m5
G C C G A G
C G G C T C
m5 m5
|
|
NotI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2055 |
m5
G C G G C C G C
C G C C G G C G
m5
|
| 2057 |
m5
G C G G C C G C
C G C C G G C G
m5 m5
|
| 1310 |
m5 m5
G C G G C C G C
C G C C G G C G
m5 m5
|
| 4096 |
m5 m5m5 m5
G C G G C C G C
C G C C G G C G
m5 m5m5 m5
|
| 2059 |
m5 m5
G C G G C C G C
C G C C G G C G
m5 m5
|
| 2058 |
m5
G C G G C C G C
C G C C G G C G
m5 m5
|
|
NruI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1952 |
m5
T C G C G A
A G C G C T
|
| 1563 |
m5
T C G C G A
A G C G C T
m5
|
| 587 |
m5 m5
T C G C G A
A G C G C T
m5 m5
|
| 3457 |
m5
T C G C G A
A G C G C T
|
| 1953 |
m5
T C G C G A
A G C G C T
|
| 1954 |
m5
T C G C G A
A G C G C T
m5
|
|
NsbI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1134 |
m5
T G C G C A
A C G C G T
m5
|
|
NspV |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 890 |
m5
T T C G A A
A A G C T T
m5
|
|
PaeR7I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4098 |
m5 m5
C T C G A G
G A G C T C
m5 m5
|
| 812 |
m5
C T C G A G
G A G C T C
m5
|
| 1160 |
m5
C T C G A G
G A G C T C
m5
|
|
PauI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1297 |
m5 m5
G C G C G C
C G C G C G
m5 m5
|
|
PdiI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1298 |
m5
G C C G G C
C G G C C G
m5
|
|
Pfl23II |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1301 |
m5 m5
C G T A C G
G C A T G C
m5 m5
|
|
PluTI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 3407 |
m5
G G C G C C
C C G C G G
m5
|
| 4102 |
m5 m5m5
G G C G C C
C C G C G G
m5m5 m5
|
|
PmaCI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1603 |
m5
C A C G T G
G T G C A C
m5
|
|
PmlI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4103 |
m5 m5
C A C G T G
G T G C A C
m5 m5
|
| 814 |
m5
C A C G T G
G T G C A C
m5
|
|
Ppu21I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2558 |
m5
Y A C G T R
R T G C A Y
m5
|
|
Psp1406I |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1137 |
m5
A A C G T T
T T G C A A
m5
|
|
PspXI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4108 |
m5m5 m5
V C T C G A G B
B G A G C T C V
m5 m5m5
|
| 2494 |
m5
V C T C G A G B
B G A G C T C V
m5
|
| 2495 |
m5
V C T C G A G B
B G A G C T C V
m5 m5
|
| 2496 |
m5 m5
V C T C G A G B
B G A G C T C V
m5 m5
|
|
PvuI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2013 |
m5
C G A T C G
G C T A G C
|
| 2015 |
m5
C G A T C G
G C T A G C
m5
|
| 815 |
m5 m5
C G A T C G
G C T A G C
m5 m5
|
| 4110 |
m5 m5
C G A T C G
G C T A G C
m5 m5
|
| 2014 |
m5
C G A T C G
G C T A G C
|
| 1476 |
m5
C G A T C G
G C T A G C
m5
|
|
RigI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 3512 |
m5
G G C C G G C C
C C G G C C G G
m5
|
|
RsrII |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2139 |
m5
C G G W C C G
G C C W G G C
|
| 2140 |
m5
C G G W C C G
G C C W G G C
m5
|
| 816 |
m5 m5
C G G W C C G
G C C W G G C
m5 m5
|
| 4113 |
m5 m5m5
C G G W C C G
G C C W G G C
m5m5 m5
|
|
SacII |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 310 |
m5m5 m5
C C G C G G
G G C G C C
|
| 4115 |
m5m5 m5
C C G C G G
G G C G C C
m5 m5m5
|
| 2100 |
m5
C C G C G G
G G C G C C
|
| 2102 |
m5
C C G C G G
G G C G C C
m5
|
| 1570 |
m5
C C G C G G
G G C G C C
m5
|
| 1239 |
m5 m5
C C G C G G
G G C G C C
m5 m5
|
| 2101 |
m5
C C G C G G
G G C G C C
|
|
SalI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2141 |
m5
G T C G A C
C A G C T G
|
| 2019 |
m5
G T C G A C
C A G C T G
|
| 818 |
m5
G T C G A C
C A G C T G
m5
|
| 311 |
m5 m5
G T C G A C
C A G C T G
|
| 4116 |
m5 m5
G T C G A C
C A G C T G
m5 m5
|
| 2142 |
m5
G T C G A C
C A G C T G
m5
|
|
SalDI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1571 |
m5
T C G C G A
A G C G C T
m5
|
|
Sbo13I | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1572 |
m5
T C G C G A
A G C G C T
m5
|
|
SfoI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1865 |
m5
G G C G C C
C C G C G G
m5
|
| 1901 |
m5 m5
G G C G C C
C C G C G G
m5
|
| 4126 |
m5 m5m5
G G C G C C
C C G C G G
m5m5 m5
|
|
SfuI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1575 |
m5
T T C G A A
A A G C T T
m5
|
|
SgfI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1241 |
m5
G C G A T C G C
C G C T A G C G
m5
|
| 1688 |
m5 m5
G C G A T C G C
C G C T A G C G
m5 m5
|
| 1687 |
m5
G C G A T C G C
C G C T A G C G
m5
|
|
SgrAI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4127 |
m5 m5m5 m5
C R C C G G Y G
G Y G G C C R C
m5 m5m5 m5
|
| 1417 |
m5
C R C C G G Y G
G Y G G C C R C
m5
|
|
SgrBI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2403 |
m5
C C G C G G
G G C G C C
m5
|
| 2404 |
m5 m5
C C G C G G
G G C G C C
m5 m5
|
|
SgsI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2559 |
m5 m5
G G C G C G C C
C C G C G C G G
m5 m5
|
|
SlaI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2418 |
m5
C T C G A G
G A G C T C
m5
|
|
SmaI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 312 |
m5m5m5
C C C G G G
G G G C C C
|
| 4128 |
m5m5m5
C C C G G G
G G G C C C
m5m5m5
|
| 750 |
m5
C C C G G G
G G G C C C
m5
|
|
SmuI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2562 |
m5
C C C G C
G G G C G
m5
|
|
SnaBI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 824 |
m5
T A C G T A
A T G C A T
m5
|
|
SplI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1577 |
m5
C G T A C G
G C A T G C
|
|
SpoI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1579 |
m5
T C G C G A
A G C G C T
m5
|
|
SrfI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4131 |
m5m5m5 m5
G C C C G G G C
C G G G C C C G
m5 m5m5m5
|
| 3568 |
m5
G C C C G G G C
C G G G C C C G
m5
|
| 3570 |
m5 m5
G C C C G G G C
C G G G C C C G
m5
|
| 3571 |
m5 m5
G C C C G G G C
C G G G C C C G
m5 m5
|
|
SsiI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2560 |
m5
C C G C
G G C G
m5
|
|
SstII | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1444 |
m5m5 m5
C C G C G G
G G C G C C
m5 m5m5
|
| 1581 |
m5
C C G C G G
G G C G C C
m5
|
|
TaiI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1142 |
m5
A C G T
T G C A
m5
|
|
TaqI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 343 |
m5
T C G A
A G C T
|
| 398 |
m5
T C G A
A G C T
m5
|
|
ThaI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 895 |
m5
C G C G
G C G C
m5
|
| 322 |
m5 m5
C G C G
G C G C
|
| 604 |
m5 m5
C G C G
G C G C
m5 m5
|
| 705 |
m5
C G C G
G C G C
m5
|
|
TliI | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 1399 |
m5
C T C G A G
G A G C T C
m5
|
|
TspMI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4136 |
m5m5m5
C C C G G G
G G G C C C
m5m5m5
|
| 2466 |
m5
C C C G G G
G G G C C C
m5
|
|
XhoI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 316 |
m5 m5
C T C G A G
G A G C T C
|
| 4139 |
m5 m5
C T C G A G
G A G C T C
m5 m5
|
| 1738 |
m5
C T C G A G
G A G C T C
|
| 1488 |
m5
C T C G A G
G A G C T C
m5
|
|
XmaI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 4140 |
m5m5m5
C C C G G G
G G G C C C
m5m5m5
|
| 765 |
m5
C C C G G G
G G G C C C
|
| 829 |
m5
C C C G G G
G G G C C C
m5
|
| 759 |
m5
C C C G G G
G G G C C C
m5
|
|
XorII | |
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 619 |
m5 m5
C G A T C G
G C T A G C
m5 m5
|
|
ZraI |
|
| MS#: | Sites Cleavage Sites cut impaired not cut |
| 2382 |
m5
G A C G T C
C T G C A G
m5
|
| 2386 |
m5
G A C G T C
C T G C A G
m5
|
| 4142 |
m5 m5
G A C G T C
C T G C A G
m5 m5
|