In situ continuous monitoring of bacterial biofilms has been a challenging job so far, but it is ... more In situ continuous monitoring of bacterial biofilms has been a challenging job so far, but it is fundamental to the screening of novel anti-biofilm reagents.
Green synthesis of nanoparticles is becoming a method of choice for biological research due to it... more Green synthesis of nanoparticles is becoming a method of choice for biological research due to its environmentally benign outcomes, stability and ease of synthesis. In this study, silver nanoparticles (AgNPs) were synthesized using stem (S-AgNPs), root (R-AgNPs) and mixture of stem and root (RS-AgNPs) of Delphinium uncinatum. The synthesized nanoparticles were characterized by standardized techniques and evaluated for their antioxidant, enzyme inhibition, cytotoxic and antimicrobial potentials. The AgNPs exhibited efficient antioxidant activities and considerable enzyme inhibition potential against alpha amylase, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. S-AgNPs showed strong cytotoxicity against human hepato-cellular carcinoma cells (HepG2) and high enzyme inhibitory effect (IC50 values 27.5μg/ml for AChE and 22.60 μg/ml for BChE) compared to R-AgNPs and RS-AgNPs. RS-AgNPs showed significant inhibition of Klebsiella pneumoniae and Aspergillus flavus and ...
Objective: To perform comparative diagnostic study with rotavirus infected stool samples for effi... more Objective: To perform comparative diagnostic study with rotavirus infected stool samples for efficacy ofindigenously prepared molecular transport medium (IMTM).Study Design: It was an experimental study.Place and Duration of Study: The study was conducted at Department of Microbiology of University of Karachi,Karachi Pakistan from June to July 2021.Materials and Methods: The current study was conducted based on hospital based surveillance of rotavirusgastroenteritis among children <5 years of age, stool samples were collected in commercially available viraltransport medium. Samples were known rotavirus positive /negative through PREMIER Rota clone Enzyme-Linked Immunosorbent Assay (ELISA) kit diagnostic test. Total 64 stool samples were selected to evaluate theefficacy of indigenous molecular transport medium (IMTM). Stool samples were spiked in IMTM andcommercial available viral transport medium (CVTM). RNA extraction and Real-time PCR were performed byQIAmp and in house assay r...
The current pandemic of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory s... more The current pandemic of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has already become a global threat to the human population. Infection with SARS-CoV-2 leads to a wide spectrum of clinical manifestations. Ocular abnormalities have been reported in association with COVID-19, but the nature of the impairments was not specified. Here, we report a case of a female patient diagnosed with glaucoma on re-hospitalization for ocular complications two months after being discharged from the hospital upon recovery from COVID-19. Meanwhile, the patient was found re-positive for SARS-CoV-2 in the upper respiratory tract. The infection was also diagnosed in the aqueous humor through immunostaining with antibodies against the N protein and S protein of SARS-CoV-2. Considering the eye is an immune-privileged site, we speculate that SARS-CoV-2 survived in the eye and resulted in the patient testing re-positive for SARS-CoV-2.
Globally, malaria is one of the major health problems particularly in developing countries includ... more Globally, malaria is one of the major health problems particularly in developing countries including Pakistan. This study design with aimed to find out the prevalence of malaria in human population of District Mardan, Khyber Pakhtunkhwa, Pakistan. A descriptive study was conducted from 1 January to 31 December, st st 2013. The data were collected from different health care centers of the entire district. For statistical analysis PHStat2 software version 2.5 was used, applied Chi-square test. P-value below 0.05 was considered as significant. A total of 11272 malarial suspected patients were enrolled. Among the total cases (6.8%) were positive comprises male to female ratio was (43.73%) and (56.27%) respectively. Among the positive cases (92.56%) were found Plasmodium vivax and (7.44%) were Plasmodium falciparum. On the basis of age groups high number of cases (47.4%) was observed in age between 0-20 years. Prevalence rate of malaria was found high (3.25%) in Tehsil Mardan. The highes...
Since the emergence of SARS-CoV-2, numerous studies have been attempting to determine biomarkers,... more Since the emergence of SARS-CoV-2, numerous studies have been attempting to determine biomarkers, which could rapidly and efficiently predict COVID-19 severity, however there is lack of consensus on a specific one. This retrospective cohort study is a comprehensive analysis of the initial symptoms, comorbidities and laboratory evaluation of patients, diagnosed with COVID-19 in Huoshenshan Hospital, Wuhan, from 4th February to 12th March, 2020. Based on the data collected from 63 severely ill patients from the onset of symptoms till the full recovery or demise, we found not only age (average 70) but also blood indicators as significant risk factors associated with multiple organ failure. The blood indices of all patients showed hepatic, renal, cardiac and hematopoietic dysfunction with imbalanced coagulatory biomarkers. We noticed that the levels of LDH (85%, P < .001), HBDH (76%, P < .001) and CRP (65%, P < .001) were significantly elevated in deceased patients, indicating ...
The genus Artemisia belongs to family Asteraceae and commonly used for ailments of multiple letha... more The genus Artemisia belongs to family Asteraceae and commonly used for ailments of multiple lethal diseases. Twenty-nine species of the genus have been identified from Pakistan which are widely used as pharmaceutical, agricultural, cosmetics, sanitary, perfumes and food industries. In this review we studied the medicinal uses, taxonomy, essential oils as well as phytochemistry were compiled. Data was collected from the original research articles, texts books and review papers including globally accepted search engines i.e. PubMed, ScienceDirect, Scopus, Google Scholar and Web of Science. Species found of Artemisia in Pakistan with their medicinal properties and phytochemicals were recorded. The present review highlights the geographical distribution, morphological features and medicinal importance of different Artemisia species. Species of the genus are used in different traditional treatments by the local communities of Pakistan and worldwide. The essential oils and some other impo...
In situ continuous monitoring of bacterial biofilms has been a challenging job so far, but it is ... more In situ continuous monitoring of bacterial biofilms has been a challenging job so far, but it is fundamental to the screening of novel anti-biofilm reagents.
Green synthesis of nanoparticles is becoming a method of choice for biological research due to it... more Green synthesis of nanoparticles is becoming a method of choice for biological research due to its environmentally benign outcomes, stability and ease of synthesis. In this study, silver nanoparticles (AgNPs) were synthesized using stem (S-AgNPs), root (R-AgNPs) and mixture of stem and root (RS-AgNPs) of Delphinium uncinatum. The synthesized nanoparticles were characterized by standardized techniques and evaluated for their antioxidant, enzyme inhibition, cytotoxic and antimicrobial potentials. The AgNPs exhibited efficient antioxidant activities and considerable enzyme inhibition potential against alpha amylase, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. S-AgNPs showed strong cytotoxicity against human hepato-cellular carcinoma cells (HepG2) and high enzyme inhibitory effect (IC50 values 27.5μg/ml for AChE and 22.60 μg/ml for BChE) compared to R-AgNPs and RS-AgNPs. RS-AgNPs showed significant inhibition of Klebsiella pneumoniae and Aspergillus flavus and ...
Objective: To perform comparative diagnostic study with rotavirus infected stool samples for effi... more Objective: To perform comparative diagnostic study with rotavirus infected stool samples for efficacy ofindigenously prepared molecular transport medium (IMTM).Study Design: It was an experimental study.Place and Duration of Study: The study was conducted at Department of Microbiology of University of Karachi,Karachi Pakistan from June to July 2021.Materials and Methods: The current study was conducted based on hospital based surveillance of rotavirusgastroenteritis among children <5 years of age, stool samples were collected in commercially available viraltransport medium. Samples were known rotavirus positive /negative through PREMIER Rota clone Enzyme-Linked Immunosorbent Assay (ELISA) kit diagnostic test. Total 64 stool samples were selected to evaluate theefficacy of indigenous molecular transport medium (IMTM). Stool samples were spiked in IMTM andcommercial available viral transport medium (CVTM). RNA extraction and Real-time PCR were performed byQIAmp and in house assay r...
The current pandemic of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory s... more The current pandemic of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has already become a global threat to the human population. Infection with SARS-CoV-2 leads to a wide spectrum of clinical manifestations. Ocular abnormalities have been reported in association with COVID-19, but the nature of the impairments was not specified. Here, we report a case of a female patient diagnosed with glaucoma on re-hospitalization for ocular complications two months after being discharged from the hospital upon recovery from COVID-19. Meanwhile, the patient was found re-positive for SARS-CoV-2 in the upper respiratory tract. The infection was also diagnosed in the aqueous humor through immunostaining with antibodies against the N protein and S protein of SARS-CoV-2. Considering the eye is an immune-privileged site, we speculate that SARS-CoV-2 survived in the eye and resulted in the patient testing re-positive for SARS-CoV-2.
Globally, malaria is one of the major health problems particularly in developing countries includ... more Globally, malaria is one of the major health problems particularly in developing countries including Pakistan. This study design with aimed to find out the prevalence of malaria in human population of District Mardan, Khyber Pakhtunkhwa, Pakistan. A descriptive study was conducted from 1 January to 31 December, st st 2013. The data were collected from different health care centers of the entire district. For statistical analysis PHStat2 software version 2.5 was used, applied Chi-square test. P-value below 0.05 was considered as significant. A total of 11272 malarial suspected patients were enrolled. Among the total cases (6.8%) were positive comprises male to female ratio was (43.73%) and (56.27%) respectively. Among the positive cases (92.56%) were found Plasmodium vivax and (7.44%) were Plasmodium falciparum. On the basis of age groups high number of cases (47.4%) was observed in age between 0-20 years. Prevalence rate of malaria was found high (3.25%) in Tehsil Mardan. The highes...
Since the emergence of SARS-CoV-2, numerous studies have been attempting to determine biomarkers,... more Since the emergence of SARS-CoV-2, numerous studies have been attempting to determine biomarkers, which could rapidly and efficiently predict COVID-19 severity, however there is lack of consensus on a specific one. This retrospective cohort study is a comprehensive analysis of the initial symptoms, comorbidities and laboratory evaluation of patients, diagnosed with COVID-19 in Huoshenshan Hospital, Wuhan, from 4th February to 12th March, 2020. Based on the data collected from 63 severely ill patients from the onset of symptoms till the full recovery or demise, we found not only age (average 70) but also blood indicators as significant risk factors associated with multiple organ failure. The blood indices of all patients showed hepatic, renal, cardiac and hematopoietic dysfunction with imbalanced coagulatory biomarkers. We noticed that the levels of LDH (85%, P < .001), HBDH (76%, P < .001) and CRP (65%, P < .001) were significantly elevated in deceased patients, indicating ...
The genus Artemisia belongs to family Asteraceae and commonly used for ailments of multiple letha... more The genus Artemisia belongs to family Asteraceae and commonly used for ailments of multiple lethal diseases. Twenty-nine species of the genus have been identified from Pakistan which are widely used as pharmaceutical, agricultural, cosmetics, sanitary, perfumes and food industries. In this review we studied the medicinal uses, taxonomy, essential oils as well as phytochemistry were compiled. Data was collected from the original research articles, texts books and review papers including globally accepted search engines i.e. PubMed, ScienceDirect, Scopus, Google Scholar and Web of Science. Species found of Artemisia in Pakistan with their medicinal properties and phytochemicals were recorded. The present review highlights the geographical distribution, morphological features and medicinal importance of different Artemisia species. Species of the genus are used in different traditional treatments by the local communities of Pakistan and worldwide. The essential oils and some other impo...
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