Rieckmann et al., 1995 - Google Patents

Semi-quantitative analysis of cytokine gene expression in blood and cerebrospinal fluid cells by reverse transcriptase polymerase chain reaction

Rieckmann et al., 1995

Document ID
16927785733055770460
Author
Rieckmann P
Albrecht M
Ehrenreich H
Weber T
Michel U
Publication year
Publication venue
Research in Experimental Medicine

External Links

Snippet

An easy, reproducible and semi-quantitative, non-radioactive method for the analysis of mRNA expression for various cytokines,(ie, Interleukin (IL)-1β, IL-4, IL-6, tumor necrosis factor (TNF)-α, lymphotoxin (LT), transforming growth factor (TGF)-β, interferon (IFN)-γ and …
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C12Q1/6851—Quantitative amplification
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Hybridisation probes
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    • C12Q2600/00—Oligonucleotides characterized by their use
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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