Yang et al., 2014 - Google Patents

Permanent genetic memory with> 1-byte capacity

Yang et al., 2014

View HTML
Document ID
16386907549420322295
Author
Yang L
Nielsen A
Fernandez-Rodriguez J
McClune C
Laub M
Lu T
Voigt C
Publication year
Publication venue
Nature methods

External Links

Snippet

Genetic memory enables the recording of information in the DNA of living cells. Memory can record a transient environmental signal or cell state that is then recalled at a later time. Permanent memory is implemented using irreversible recombinases that invert the …
Continue reading at www.ncbi.nlm.nih.gov (HTML) (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING; COUNTING
    • G06F—ELECTRICAL DIGITAL DATA PROCESSING
    • G06F19/00—Digital computing or data processing equipment or methods, specially adapted for specific applications
    • G06F19/10—Bioinformatics, i.e. methods or systems for genetic or protein-related data processing in computational molecular biology
    • G06F19/12—Bioinformatics, i.e. methods or systems for genetic or protein-related data processing in computational molecular biology for modelling or simulation in systems biology, e.g. probabilistic or dynamic models, gene-regulatory networks, protein interaction networks or metabolic networks
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

Similar Documents

Publication Publication Date Title
Yang et al. Permanent genetic memory with> 1-byte capacity
Nielsen et al. Multi‐input CRISPR/C as genetic circuits that interface host regulatory networks
Siuti et al. Synthetic circuits integrating logic and memory in living cells
Kim et al. De novo-designed translation-repressing riboregulators for multi-input cellular logic
Chatterjee et al. Minimal PAM specificity of a highly similar SpCas9 ortholog
Roquet et al. Synthetic recombinase-based state machines in living cells
Pham et al. Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes
Farzadfard et al. Single-nucleotide-resolution computing and memory in living cells
Schmidt et al. Transcriptional recording by CRISPR spacer acquisition from RNA
Chan et al. 'Deadman'and'Passcode'microbial kill switches for bacterial containment
Silpe et al. The bacterial toxin colibactin triggers prophage induction
Mutalik et al. Quantitative estimation of activity and quality for collections of functional genetic elements
Jensen et al. Antibiotics disrupt coordination between transcriptional and phenotypic stress responses in pathogenic bacteria
US10607716B2 (en) Synthetic biology tools
Siuti et al. Engineering genetic circuits that compute and remember
Bhattarai-Kline et al. Recording gene expression order in DNA by CRISPR addition of retron barcodes
Yu et al. Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systems
Guiziou et al. Hierarchical composition of reliable recombinase logic devices
Wong et al. Layering genetic circuits to build a single cell, bacterial half adder
Rovinskiy et al. Supercoil levels in E. coli and Salmonella chromosomes are regulated by the C-terminal 35–38 amino acids of GyrA
Zúñiga et al. Rational programming of history-dependent logic in cellular populations
Gallagher et al. Genome-wide protein–DNA interaction site mapping in bacteria using a double-stranded DNA-specific cytosine deaminase
Mienda et al. Genome-scale metabolic models as platforms for identification of novel genes as antimicrobial drug targets
Fouhy et al. Proteomics as the final step in the functional metagenomics study of antimicrobial resistance
Liu et al. Identification of strong promoters based on the transcriptome of Bacillus licheniformis