Leigh et al., 1985 - Google Patents

Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules.

Leigh et al., 1985

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Document ID
10040851626190160633
Author
Leigh J
Signer E
Walker G
Publication year
Publication venue
Proceedings of the National Academy of Sciences

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By screening with the fluorescent stain Calcofluor, we have isolated 26 independent transposon Tn5 insertion mutants of Rhizobium meliloti that are deficient in the production of a known extracellular polysaccharide (Exo-). The mutants belonged to six distinct genetic …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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