WO2018006462A1 - Fungicidal composition - Google Patents

Abstract

Provided is a fungicidal composition, comprising two active ingredients A and B. The active ingredient A is a compound having a structure represented by formula (I); the active ingredient B is sulphur; the weight ratio between the two ingredients is 1:5-300. Also provided are a preparation method for the composition and a use of the composition. Test results indicate that the fungicidal composition features an obvious synergistic effect, reduced administration amounts of single doses, and reduced costs, and is capable of effectively preventing and treating particular fungal diseases of crops. Co-formulating fungicides with different action mechanisms and action approaches has good effects in the broadening of the fungi spectrum, the reduction of the fungal resistance, the improvement of the prevention and treatment effect, and other aspects.

Description

Fungicide composition Technical field

The invention belongs to the field of agricultural plant protection, in particular to a germicidal composition with improved properties, in particular to a germicidal composition comprising benzisothiazolinones and sulfur.

Background technique

Benzoisothiazolinones are a new type of broad-spectrum fungicide mainly used for the prevention and treatment of various bacterial and fungal diseases such as cereal crops, vegetables and fruits. The mechanism of bactericidal action mainly includes destroying the nuclear structure of the pathogen, causing it to lose the heart part and failing to death and interfering with the metabolism of the pathogenic cells, causing its physiological disorder and ultimately leading to death. It can effectively protect plants from pathogens in the early stage of disease occurrence. Increasing the dosage after disease occurrence can obviously control the spread of pathogens, thus achieving the dual functions of protection and eradication.

Sulfur is a traditional inorganic sulfur protective fungicide. The mechanism of action is to act on the electron transfer process between the cytochromes b and c in the redox process, capture electrons, interfere with the normal oxidation-reduction reaction, and cause the bacteria to die. Applicable crops and control objects Sulfur has a wide range of applications and has good control effects on many kinds of pests and diseases. Production is mainly used to control wheat crops powdery mildew, melon powdery mildew, anthracnose, asparagus stem blight, peanut leaf spot (brown spot, black spot, net blotch, etc.), apple powdery mildew, rot disease , pear tree rot disease, powdery mildew, pear hibiscus, peach leaf disease, brown rot, anthracnose, sputum fruit disease, grape powdery mildew, felt disease, hawthorn powdery mildew, citrus scab, anthracnose, powdery mildew , rust spiders, rust spiders, flower powdery mildew, leaf spot, rubber powdery mildew and closed environment fumigation.

Practical pesticide experience has shown that repeated and specific application of an active compound to control harmful fungi will in many cases lead to rapid selectivity of fungal strains, and in order to reduce the risk of selectivity of resistant fungal strains, different activities are currently used. A mixture of compounds to control harmful fungi. By combining active compounds having different mechanisms of action, resistance can be delayed, application rates can be reduced, and cost of control can be reduced.

Summary of the invention

The object of the present invention is to solve the resistance of the bactericide in practical application and the problem of soil residue, and to screen out the fungicides with different sterilization principles to obtain a new fungicide composition, so as to improve the control effect of the bactericide and delay the resistance. Sexual production, reducing the amount of application, reducing the cost of prevention.

Another object of the present invention is to provide a preparation method comprising the two active ingredients A and B germicidal compositions and in agriculture The application of the field to control crop diseases.

The object of the invention can be achieved by the following measures:

A synergistic biocide composition comprising two active components, A and B, wherein active component A is a structural compound having formula (I) and active component B is sulfur.

In the formula (I), R is selected from H or a C ₁ - C ₈ alkyl group.

The C ₁ -C ₈ alkyl group in the present invention means a linear or branched alkyl group having 1 to 8 carbon atoms, and includes a C ₁ alkyl group (e.g., methyl group) and a C ₂ alkyl group (e.g., ethyl group). , C ₃ alkyl (such as n-propyl, isopropyl), C ₄ alkyl (such as n-butyl, isobutyl, tert-butyl, sec-butyl), C ₅ alkyl (such as n-pentyl, etc.) , C ₆ alkyl, C ₇ alkyl, C ₈ alkyl. It includes, but is not limited to, C ₁ -C ₆ alkyl, C ₁ -C ₅ alkyl, C ₁ -C ₄ alkyl, and the like.

In a preferred embodiment, R is selected from H or a C ₁ -C ₄ alkyl group.

In a more preferred embodiment, R is selected from the group consisting of H, -CH ₃ or -C ₄ H ₉ .

In the formula (I), when R is H, A is 1,2-benzisothiazolin-3-one (abbreviated as BIT in the specification).

In the formula (I), when R is -CH ₃ , A is 2-methyl-1,2-benzisothiazolin-3-one (abbreviated as MBIT in the specification).

In the formula (I), when R is -C ₄ H ₉ , A is 2-n-butyl-1,2-benzisothiazolin-3-one, and the "butyl" in the formula is n-butyl. (BBIT in the specification).

The inventors have found through experiments that the composition of the present invention is effective for controlling bacterial or fungal diseases of crops, and more importantly, the application amount is reduced and the use cost is lowered. The compounds containing component A and component B have different structural types and different mechanisms of action. The combination of the two can expand the bactericidal spectrum, and can delay the generation and development of pathogen resistance to a certain extent, and component A and group There is no cross-resistance between points B.

The weight ratio between the two components in the bactericide composition of the present invention is 1:5 to 300, preferably 1:5 to 250, in order to make the synergistic effect between the two components more remarkable, the component A and The weight ratio between the two components of component B can be further optimized to 1:10 to 250.

In a preferred embodiment, the weight ratio between the two components of A and B can be arbitrarily adjusted within the range of the following ratios: 1:5, 1:6, 1:7, 1:8, 1:9 , 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:21, 1 :22, 1:23, 1:24, 1:25, 1: 26, 1:27, 1:28, 1:29, 1:30, 1:32, 1:33, 1:34, 1:35, 1:36, 1:37, 1:38, 1:39, 1:40, 1:41, 1:42, 1:43, 1:44, 1:45, 1:46, 1:47, 1:48, 1:49, 1:50, 1:51, 1: 52, 1:53, 1:54, 1:55, 1:56, 1:57, 1:58, 159:, 1:60, 1:61, 1:62, 1:63, 1:64, 1 : 65, 1:66, 1:67, 1:68, 1:69, 1:70, 1:71, 1:72, 1:73, 1:74, 1:75, 1:76, 1:77 , 1:78, 1:79, 1:80, 1:81, 1:82, 1:83, 1:84, 1:85, 1:86, 1:87, 1:88, 1:89, 1 :90, 1:91, 1:92, 1:93, 1:94, 1:95, 1:96, 1:97, 1:98, 1:99, 1:100, 1:10, 1:120 , 1:130, 1:140, 1:150, 1:160, 1:170, 1:180, 1:190, 1:200, 1:210, 1:220, 1:230, 1:240, 1 : 250, 1:260, 1:270, 1:280, 1:290, 1:300 can also be selected within the range of any two of the above ratios.

The composition of the present invention can be made into a pesticide-acceptable dosage form from the active ingredient and agrochemical adjuvant or adjuvant. Further, the composition comprises from 5 to 80% by weight of the active ingredient and from 95 to 20% by weight of the pesticide adjuvant to form a pesticide-acceptable dosage form.

The present invention provides the use of a bactericidal composition comprising component A and component B for controlling crop diseases in the agricultural sector, in particular for controlling fungi or bacteria of certain crops.

The above composition may specifically comprise an agrochemical adjuvant or an auxiliary such as one or more of a carrier, a solvent, a dispersing agent, a wetting agent, an adhesive, a thickener, a binder, a surfactant or a fertilizer. Common auxiliaries can be mixed during the application.

Suitable auxiliaries or auxiliaries may be solid or liquid, they are usually materials commonly used in the processing of dosage forms, such as natural or regenerated minerals, solvents, dispersants, wetting agents, adhesives, thickeners, binders. .

The method of application of the compositions of the invention comprises the use of the compositions of the invention for aerial parts of plants, in particular leaves or foliage. You can choose to soak or apply to the surface of the control object. The frequency of administration and the amount administered will depend on the biological and climatic conditions of the pathogen. The plant growth site, such as rice fields, may be wetted with a liquid formulation of the composition, or the composition may be applied to the soil in solid form, such as in the form of granules (soil application), the composition may be passed from the soil to the plant through the roots of the plant. In vivo (systemic action).

The composition of the present invention can be prepared into various pesticide-acceptable dosage forms including, but not limited to, emulsifiable concentrates, suspending agents, wettable powders, water-dispersible granules, powders, granules, aqueous preparations, aqueous emulsions, microemulsions, poison baits. A mother liquor, a mother powder or the like. In a preferred embodiment, the dosage form of the invention employs a wettable powder, a suspending agent, a water-dispersible granule, an aqueous emulsion or a microemulsion. Depending on the nature of the compositions and the intended purpose and environmental conditions to which the compositions are to be applied, the compositions may be applied by spraying, misting, dusting, spreading or pouring, and the like.

The composition of the present invention can be prepared into various dosage forms by known methods, and the active ingredient and auxiliary agent can be used, for example. The solvent and the solid carrier can be uniformly mixed and ground with a surfactant as needed to prepare a desired dosage form.

The above solvent may be selected from aromatic hydrocarbons, preferably containing from 8 to 12 carbon atoms, such as a mixture of xylenes or substituted benzenes, phthalates such as dibutyl phthalate or dicaprylic acid, aliphatic hydrocarbons such as rings. Alkenes or paraffins, alcohols and glycols and their ethers and esters, such as ethanol, ethylene glycol, ethylene glycol monomethyl; ketones, such as cyclohexanone, highly polar solvents such as N-methyl-2 Pyrrolidone, dimethyl sulfoxide or dimethylformamide, and vegetable or vegetable oils such as soybean oil.

The above solid carriers, such as those used in powders and dispersibles, are typically natural mineral fillers such as talc, kaolin, montmorillonite or activated clay. In order to manage the physical properties of the composition, it is also possible to add a highly dispersible silicic acid or a highly dispersible adsorbent polymer carrier, such as a particulate adsorbent carrier or a non-adsorbing carrier, and a suitable particulate adsorbent carrier is porous, such as pumice, bentonite or Bentonite; a suitable non-adsorbing carrier such as calcite or sand. In addition, a large amount of pre-granulated materials of inorganic or organic nature can be used as a carrier, in particular dolomite.

Suitable surfactants according to the chemical nature of the active ingredient in the composition of the present invention are lignosulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, alkaline earth metal or amine salts, alkylarylsulfonates, alkyl groups Sulfates, alkyl sulfonates, fatty alcohol sulphates, fatty acids and sulfated fatty alcohol glycol ethers, as well as condensates of sulfonated naphthalene and naphthalene derivatives with formaldehyde, naphthalene or naphthalene sulfonic acid with phenol and formaldehyde Condensate, polyoxyethylene octyl phenyl ether, ethoxylated isooctyl phenol, octyl phenol, nonyl phenol, alkyl aryl polyglycol ether, tributyl benzene polyglycol ether, three Stearyl phenyl polyglycol ether, alkyl aryl polyether alcohol, ethoxylated castor oil, polyoxyethylene alkyl ether, ethylene oxide condensate, ethoxylated polyoxypropylene, lauric acid poly Glycol ether acetal, sorbitol ester, lignin sulfite waste liquor and methyl cellulose.

In the preparation of the liquid dosage form, the active component A may be first dissolved in a basic substance to form a benzisothiazolin metal salt. Suitable basic substances include: alkali metal carbonates, alkali metal hydroxides (such as hydroxides). Sodium, potassium hydroxide), alkali metal alkoxy carbonate, alkali metal alkoxide or magnesium methoxide.

The two active ingredients in the compositions of the present invention exhibit synergistic effects, the activity of which is more pronounced than the expected sum of activity using a single compound, and the individual activity of a single compound. The synergistic effect is manifested by allowing for a reduced application rate, a broader fungicidal control profile, quicker effect, longer lasting control effect, better control of plant harmful fungi by only one or a few applications, and broadening of possible application. Intervals. These properties are particularly desirable in the practice of plant fungi control.

The fungicide composition of the invention can be applied to the field of agricultural diseases for controlling crop diseases, and the specific diseases targeted include, but not limited to, wheat diseases (such as wheat scab, wheat powdery mildew, wheat rust, etc.) and rice diseases (such as: Rice sheath blight, rice blast disease, rice blast, rice bacterial streaks, etc., corn diseases (such as: corn smut, corn big spot, corn spot), cucumber diseases (such as: cucumber frost Mildew, cucumber powdery mildew, cucumber anthracnose, cucumber Bacterial keratosis, etc., tomato diseases (eg, tomato anthracnose, tomato gray mold, tomato bacterial angular plaque, etc.), grape diseases (eg, grape downy mildew, grape gray mold, grape powdery mildew) Apple diseases (such as apple ring disease, apple anthracnose, etc.), citrus diseases (citrus canker, citrus sores, citrus anthracnose), tobacco diseases (tobacco wildfire).

The other characteristics exhibited by the bactericidal composition of the present invention are mainly as follows: 1. The compounding of the composition of the present invention has obvious synergistic effect; 2. Since the chemical composition of the two single agents of the present composition is greatly different, the effect The mechanism is completely different, there is no cross-resistance, and the problem of resistance caused by the separate use of the two single agents can be delayed; 3. The composition of the present invention is safe and safe for crops. It has been proved by experiments that the bactericidal composition of the invention has stable chemical properties, remarkable synergistic effect, and exhibits obvious synergistic effect and complementary effect on the control object. 4. This product has good preventive and therapeutic effects on fungi and bacteria.

detailed description

In order to make the objects, technical solutions and advantages of the present invention more comprehensible, the present invention will be further described in detail below with reference to the embodiments. It is understood that the specific embodiments described herein are intended to be illustrative of the invention and are not to be construed as limiting the scope of the invention. Within the scope of protection of the present invention.

The percentages in all formulations in the following examples are percentages by weight. The processing techniques of the various formulations of the compositions of the present invention are all prior art and may vary depending on the circumstances.

First, the dosage form preparation example

(1) Processing and examples of wettable powders

The active ingredient A and the active ingredient B are sufficiently mixed with various auxiliary agents and fillers, and are pulverized by an ultrafine pulverizer to obtain a wettable powder.

1. Active component A (BIT) and active component B to prepare wettable powder

Example 1:16% BIT·Sulphur Wettable Powder

BIT 1%, sulfur 15%, bentonite 4%, alkyl sulfate 5%, white carbon black 5%, kaolin to 100%.

Example 2: 25.5% BIT·Sulphur Wettable Powder

BIT 0.5%, sulfur 25%, sodium naphthalene sulfonate 5%, alkyl polyoxyethyl ether sulfonate 5%, white carbon black 6%, diatomaceous earth to 100%.

Example 3: 44% BIT·Sulfur Wettable Powder

BIT 0.25%, sulfur 43.75%, pull-open powder 5%, bentonite 2.0%, fatty alcohol sulfate 4%, white carbon black to 100%.

Example 4: 56.5% BIT·sulfur WP

BIT 0.25%, sulfur 56.25%, sodium lignosulfonate 5%, alkyl polyoxyethyl ether sulfonate 5%, white carbon black 10%, kaolin to 100%.

Example 5: 30.1% BIT·Sulfur Wettable Powder

BIT 0.1%, sulfur 30%, bentonite 3%, sodium phenolsulfonate 4%, alkyl sulfonate 3%, white carbon black to 100%.

2. Preparation of suspending agent from active ingredient A (MBIT) and active ingredient B

Example 6: 16% MBIT·sulfur WP

MBIT 1%, sulfur 15%, and the remaining components were prepared in accordance with the procedure of Example 1.

Example 7: 25.5% MBIT·sulfur WP

MBIT was 0.5%, sulfur was 25%, and the remaining components were prepared in the same manner as in Example 2.

Example 8: 44% MBIT·sulfur WP

The MBIT was 0.25% and the sulfur was 43.75%. The remaining components were prepared in the same manner as in Example 3.

Example 9: 56.5% MBIT·sulfur WP

The MBIT was 0.25% and the sulfur was 56.25%. The remaining components were prepared in the same manner as in Example 4.

Example 10: 30.1% MBIT·sulfur WP

MBIT 0.1%, sulfur 30%, and the remaining components were prepared in accordance with the procedure of Example 5.

3. Active component A (BBIT) and active component B to prepare wettable powder

Example 11: 16% BBIT·sulfur WP

BBIT 1%, sulfur 15%, and the remaining components were prepared in accordance with the procedure of Example 1.

Example 12: 25.5% BBIT·Sulfur Wettable Powder

BBIT 0.5%, sulfur 25%, and the remaining components were prepared in the same manner as in Example 2.

Example 13: 44% BBIT·Sulphur Wettable Powder

BBIT 0.25%, sulfur 43.75%, and the remaining components were prepared in accordance with the method of Example 3.

Example 14: 56.5% BBIT·sulfur WP

BBIT 0.25%, sulfur 56.25%, and the remaining components were prepared in accordance with the method of Example 4.

Example 15: 30.1% BBIT·Sulfur Wettable Powder

BBIT 0.1%, sulfur 30%, and the remaining components were prepared in accordance with the procedure of Example 5.

(2) Processing and examples of water-dispersible granules

The active ingredient A and the active ingredient B, and the auxiliary agent and the filler are uniformly mixed according to the formula, and are pulverized into a wettable powder by a jet stream, and then added with a certain amount of water to be mixed and granulated, and after being sieved by drying. A water dispersible granule product is obtained.

1. Active component A (BIT) and active component B to prepare water-dispersible granules

Example 16: 12% BIT·Sulphur Water Dispersible Granules

BIT 2%, sulfur 10%, sodium lauryl sulfate 6%, ammonium sulfate 6%, alkyl naphthalenesulfonate calcium 5%, light potassium carbonate to 100%.

Example 17: 76% BIT·Sulphur Water Dispersible Granules

BIT 1%, sulfur 75%, polyoxyethylene octylphenyl ether 5%, potassium lauryl naphthalene sulfonate 5%, sodium lignin sulfonate 5%, kaolin to 100%.

Example 18: 25.2% BIT·Sulphur Water Dispersible Granules

BIT 0.2%, sulfur 25%, potassium lignosulfonate 5%, calcium sulfate 3%, alkyl naphthalenesulfonate 4%, light calcium carbonate to 100%.

Example 19: 50.2% BIT·Sulphur Water Dispersible Granules

BIT 0.2%, sulfur 50%, methyl naphthalenesulfonate sodium formaldehyde condensate 5%, sodium lignosulfonate 5%, white carbon black to 100%.

2. Active component A (MBIT) and active component B to prepare water-dispersible granules

Example 20: 12% MBIT·Sulphur Water Dispersible Granules

MBIT 2%, sulfur 10%, and the remaining components were prepared in accordance with the procedure of Example 16.

Example 21: 76% MBIT·Sulphur Water Dispersible Granules

MBIT 1%, sulfur 75%, and the remaining components were prepared in accordance with the procedure of Example 17.

Example 22: 25.2% MBIT·Sulphur Water Dispersible Granules

MBIT 0.2%, sulfur 25%, and the remaining components were prepared in accordance with the procedure of Example 18.

Example 23: 50.2% MBIT·Sulphur Water Dispersible Granules

MBIT 0.2%, sulfur 50%, and the remaining components were prepared in accordance with the procedure of Example 19.

3. Active component A (BBIT) and active component B to prepare water-dispersible granules

Example 24: 12% BBIT·Sulphur Water Dispersible Granules

BBIT 2%, sulfur 10%, and the remaining components were prepared in accordance with the procedure of Example 16.

Example 25: 76% BBIT·Sulphur Water Dispersible Granules

BBIT 1%, sulfur 75%, and the remaining components were prepared in accordance with the procedure of Example 17.

Example 26: 25.2% BBIT·Sulphur Water Dispersible Granules

BBIT 0.2%, sulfur 25%, and the remaining components were prepared in accordance with the procedure of Example 18.

Example 27: 50.2% BBIT·Sulphur Water Dispersible Granules

BBIT 0.2%, sulfur 50%, and the remaining components were prepared in accordance with the procedure of Example 19.

(III) Processing and examples of suspending agents

The active ingredient A and the active ingredient B, and the components such as a dispersing agent, a wetting agent, a thickener and water are uniformly mixed according to the formulation, and after being sanded and/or sheared at a high speed, a semi-finished product is obtained. After the analysis, the water is added and uniformly filtered to obtain the finished product.

1. Active component A (BIT) and active component B to prepare suspension agent

Example 28: 15.5% BIT·Sulphur Suspension

BIT 0.5%, sulfur 15%, diatomaceous earth 5%, ethylene glycol 4%, sodium methylnaphthalene sulfonate formaldehyde condensate 6%, water to 100%.

Example 29: 325.25% BIT·Sulphur Suspension

BIT 0.25%, sulfur 25%, white carbon black 3%, ethylene glycol 5%, sodium benzenesulfonate 5%, fatty alcohol polyoxyethylene ether phosphate 5%, water to 100%.

Example 30: 37.75% BIT·Sulphur Suspension

BIT 0.25%, sulfur 37.5%, bentonite 4%, glycerol 5%, sodium lignin sulfonate 7%, xanthan gum 2%, water to 100%.

Example 31: 50.25% BIT·Sulphur Suspension

BIT 0.25%, sulfur 50%, kaolin 5%, glycerol 3%, fatty alcohol polyoxyethylene ether phosphate 5%, water to 100%.

Example 32: 27.6% BIT·Sulphur Suspension

BIT 0.1%, sulfur 27.5%, bentonite 5%, ethylene glycol 5%, sodium benzoate 4%, ethoxylated castor oil 8%, water to 100%.

2. Preparation of suspending agent from active ingredient A (MBIT) and active ingredient B

Example 33: 15.5% MBIT·Sulphur Suspension

MBIT 0.5%, sulfur 15%, and the remaining components were prepared in accordance with the procedure of Example 28.

Example 34: 25.25% MBIT·Sulphur Suspension

The MBIT was 0.25%, the sulfur was 25%, and the remaining components were prepared in the same manner as in Example 29.

Example 35: 37.75% MBIT·Sulphur Suspension

MBIT 0.25%, sulfur 37.5%, and the remaining components were prepared in accordance with the procedure of Example 30.

Example 36: 50.25% MBIT·Sulphur Suspension

MBIT was 0.25%, sulfur was 50%, and the remaining components were prepared in accordance with the procedure of Example 31.

Example 37: 27.6% MBIT·Sulphur Suspension

MBIT 0.1%, sulfur 27.5%, and the remaining components were prepared in accordance with the procedure of Example 32.

3. Preparation of suspending agent from active ingredient A (BBIT) and active ingredient B

Example 38: 15.5% BBIT·Sulphur Suspension

BBIT 0.5%, sulfur 15%, and the remaining components were prepared in accordance with the procedure of Example 28.

Example 39: 25.25% BBIT·Sulphur Suspension

BBIT 0.25%, sulfur 25%, and the remaining components were prepared in accordance with the procedure of Example 29.

Example 40: 37.75% BBIT·Sulphur Suspension

BBIT 0.25%, sulfur 37.5%, and the remaining components were prepared in accordance with the procedure of Example 30.

Example 41: 50.25% BBIT·Sulphur Suspension

MBIT was 0.25%, sulfur was 50%, and the remaining components were prepared in accordance with the procedure of Example 31.

Example 42: 27.6% BBIT·Sulphur Suspension

BBIT 0.1%, sulfur 27.5%, and the remaining components were prepared in accordance with the procedure of Example 32.

Second, the efficacy test

(1) Bioassay examples

According to the test grading standards, the incidence of the whole plant leaves was investigated, and the disease index and control effect were calculated.

The control effect is converted into the probability value (y), the liquid height (μg/ml) is converted into a logarithmic value (x), the virulence equation is calculated by the least squares method, and the neutral concentration EC50 is suppressed, and the virulence of the drug is calculated according to the method of Sun Yunpei. Exponential Co-toxicity Factor (CTC).

Measured virulence index (ATI) = (standard drug EC50 / test drug EC50) * 100

Theoretical virulence index (TTI) = A virulence index * Percentage of A in the mixture + B virulence index * Percentage of B in the mixture

Co-toxicity coefficient (CTC) = [mixture measured virulence index (ATI) / mixed theory virulence index (TTI)] * 100

CTC ≤ 80, the composition showed antagonism, 80 < CTC < 120, the composition showed additive effect, CTC ≥ 120, the composition exhibits synergism.

1, BIT and sulfur complex pairing virulence test

Table 1. Analysis of the results of BIT and sulfur complex pomegranate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
sulfur	47.66	100.00	\	\
BIT	32.54	146.47	\	\
BIT: sulfur = 1:5	27.41	173.88	138.73	125.34
BIT: sulfur = 1:15	25.19	189.19	143.57	131.78
BIT: sulfur = 1:30	24.82	192.03	144.97	132.46
BIT: sulfur = 1:50	24.48	194.70	145.56	133.76
BIT: sulfur = 1:75	24.16	197.24	145.86	135.23
BIT: sulfur = 1:100	23.73	200.85	146.01	137.56
BIT: sulfur = 1:125	23.47	203.11	146.10	139.02
BIT: sulfur = 1:150	23.59	202.07	146.16	138.25
BIT: sulfur = 1:175	23.87	199.67	146.21	136.57
BIT: sulfur = 1:200	24.16	197.23	146.24	134.87
BIT: sulfur = 1:225	24.58	193.89	146.26	132.56
BIT: sulfur = 1:250	25.05	190.26	146.28	130.06
BIT: sulfur = 1:275	26.11	182.56	146.30	124.78
BIT: sulfur = 1:300	26.48	179.95	146.32	122.99

The results (Table 1) showed that the control effect of BIT and sulphur complex on pomegranate scab was significantly improved, indicating that there was a significant synergistic effect between the two on the control of pomegranate scab. It has been found that it is difficult to have a synergistic effect when the ratio of the ratio of BIT to sulfur is outside the scope of the present invention.

2, MBIT and sulfur complex pairing toxicity test

Table 2. Analysis of the results of MBIT and sulfur complexation

Pharmacy name	EC 50 ( μg / ml)	ATI	TTI	Co-toxicity coefficient (CTC)
sulfur	34.59	100	\	\
MBIT	21.45	161.26	\	\
MBIT: sulfur = 1:5	18.88	183.25	151.05	121.32

MBIT: sulfur = 1:15	17.36	199.28	157.43	126.58
MBIT: sulfur = 1:30	16.97	203.85	159.28	127.98
MBIT: sulfur = 1:50	16.64	207.87	160.06	129.87
MBIT: sulfur = 1:75	16.37	211.27	160.45	131.67
MBIT: sulfur = 1:100	16.09	214.92	160.65	133.78
MBIT: sulfur = 1:125	15.83	218.48	160.77	135.89
MBIT: sulfur = 1:150	15.42	224.34	160.85	139.47
MBIT: sulfur = 1:175	15.49	223.33	160.91	138.79
MBIT: sulfur = 1:200	15.88	217.76	160.96	135.29
MBIT: sulfur = 1:225	16.36	211.48	160.99	131.36
MBIT: sulfur = 1:250	16.87	205.05	161.02	127.35
MBIT: sulfur = 1:275	17.23	200.78	161.04	124.68
MBIT: sulfur = 1:300	17.85	193.78	161.06	120.32

The results (Table 2) showed that the control effect of MBIT and sulphur complexes on cowpea keratosis was significantly improved, indicating that there was a significant synergistic effect between the two groups on the control of cowpea plaque. It has been found that it is difficult to have a synergistic effect when the ratio of MBIT to sulfur is not within the scope of the present invention.

3, BBIT and sulfur complex pairing virulence test

Table 3. Analysis of the results of BBIT and sulfur complexation in wheat black ear virus

Pharmacy name	EC 50 ( μg / ml)	ATI	TTI	Co-toxicity coefficient (CTC)
sulfur	40.53	100	\	\
BBIT	25.38	159.69	\	\
BBIT: sulfur = 1:5	21.59	187.76	149.74	125.39
BBIT: sulfur = 1:15	19.78	204.88	155.96	131.37
BBIT: sulfur = 1:30	19.25	210.54	157.76	133.45
BBIT: sulfur = 1:50	18.82	215.41	158.52	135.89
BBIT: sulfur = 1:75	18.48	219.32	158.90	138.02
BBIT: sulfur = 1:100	18.15	223.25	159.10	140.32
BBIT: sulfur = 1:125	17.73	228.59	159.22	143.57

BBIT: sulfur = 1:150	17.57	230.63	159.29	144.78
BBIT: sulfur = 1:175	17.80	227.70	159.35	142.89
BBIT: sulfur = 1:200	18.18	222.97	159.39	139.89
BBIT: sulfur = 1:225	18.59	218.06	159.43	136.78
BBIT: sulfur = 1:250	19.07	212.53	159.45	133.29
BBIT: sulfur = 1:275	20.04	202.29	159.47	126.85
BBIT: sulfur = 1:300	20.44	198.31	159.49	124.34

The results (Table 3) showed that the control effect of BBIT and sulfur complex wheat smut was significantly improved, indicating that there was a significant synergistic effect between the two on wheat smut control. Especially between 1:15 and 250, the synergy is very obvious. It has been found that it is difficult to have a synergistic effect when the ratio of BBIT to sulfur is not within the scope of the present invention.

(2) Field efficacy test

Test method: In the early stage of the disease, the first spray was immediately performed, and after 7 days, the second application was carried out, each treatment of 4 cells, 20 square meters per cell. The incidence of the disease was investigated before the drug and 11 days after the second drug. Each plot was randomly sampled at 5 points, and 5 crops were investigated at each point. The percentage of the lesion area per leaf on the whole plant was counted and graded. Disease index and control effect.

Expected control effect (%) = X + Y-XY / 100 (where X, Y is a single dose control)

Grading standards:

Level 0: no lesions;

Grade 1: less than 5 leaf lesions, less than 1 cm in length;

Grade 3: 6-10 leaf lesions, some lesions are longer than 1 cm;

Grade 5: 11-25 leaf lesions, some lesions are connected into pieces, and the lesion area accounts for 10-25% of the leaf area;

Grade 7: more than 26 leaf lesions, the lesions are connected into pieces, and the lesion area accounts for 26-50% of the leaf area;

Grade 9: The lesions are connected into pieces, and the area of the lesions accounts for more than 50% of the leaf area or the whole leaves are dead.

1. BIT and sulfur compounding field efficacy experiment

Table 4 BIT and sulfur mixed with apple tree powdery mildew control effect

The results of the determination (Table 4) show that the control effect of BIT and sulfur mixed with apple tree powdery mildew is significantly improved, indicating that the two have a significant synergistic effect on apple powdery mildew.

2. MBIT and sulfur compounding field efficacy experiment

Table 5 MBIT combined with sulfur to control the effect of grapefruit sore disease

The results of the determination (Table 5) showed that the control effect of MBIT combined with sulfur on grapefruit scab was significantly improved, indicating that the two had a significant synergistic effect on grapefruit scab.

3. BBIT and sulfur compounding field efficacy experiment

Table 6 BBIT and sulfur mixed with peach bacterial perforation control effect

The results of the determination (Table 6) showed that the control effect of BBIT combined with sulfur on the bacterial perforation of peach was significantly improved, indicating that the two had a significant synergistic effect on the bacterial perforation of peach.

Claims (10)

1. A bactericidal composition, characterized in that the composition comprises two active components A and B, wherein the active component A is a structural compound having the formula (I), the active component B is sulphur, and the two components The weight ratio between 1:5 and 300,

   

   In the formula (I), R is selected from H or a C ₁ - C ₈ alkyl group.
2. The bactericidal composition according to claim 1, wherein in the formula (I), R is selected from H or a C ₁ - C ₄ alkyl group.
3. The bactericidal composition according to claim 2, wherein in the formula (I), R is selected from H, -CH ₃ or -C ₄ H ₉ , and the corresponding active component A is 1,2-benzox. Isothiazolin-3-one, 2-methyl-1,2-benzisothiazolin-3-one or 2-n-butyl-1,2-benzisothiazolin-3-one.
4. The bactericide composition according to claim 1, wherein the weight ratio of the active component A to the active component B is from 1:5 to 250.
5. The bactericidal composition according to claim 4, wherein the weight ratio of the active component A to the active component B is from 1:10 to 250.
6. The bactericidal composition according to any one of claims 1 to 5, wherein the composition is made into a pesticide-acceptable dosage form from an active ingredient and an agrochemical adjuvant or an auxiliary.
7. The bactericidal composition according to claim 6, wherein the active ingredient in the composition has a mass content of from 5 to 80%.
8. The bactericidal composition according to claim 6, wherein the dosage form is a wettable powder, a suspension or a water-dispersible granule.
9. The bactericidal composition according to claim 6, wherein the pesticide adjuvant or adjuvant is selected from the group consisting of a carrier, a solvent, a dispersing agent, a wetting agent, an adhesive, a thickener, a binder, a surfactant or a fertilizer. One or several of them.
10. Use of the bactericide composition according to any one of claims 1 to 5 for controlling crop diseases in the agricultural field.