WO2009052078A1 - Ccr10 antagonists - Google Patents

Ccr10 antagonists Download PDF

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Publication number
WO2009052078A1
WO2009052078A1 PCT/US2008/079781 US2008079781W WO2009052078A1 WO 2009052078 A1 WO2009052078 A1 WO 2009052078A1 US 2008079781 W US2008079781 W US 2008079781W WO 2009052078 A1 WO2009052078 A1 WO 2009052078A1
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WIPO (PCT)
Prior art keywords
ethyl
amino
biphenyl
chloro
alkyl
Prior art date
Application number
PCT/US2008/079781
Other languages
French (fr)
Inventor
Kaka Dey
Donghong Amy Gao
Daniel R. Goldberg
Alexander Heim-Riether
John E. Mangette
Ingo Andreas Mugge
Roger Snow
Alan David Swinamer
Jiang-Ping Wu
Zhaoming Xiong
Yu Yang
Original Assignee
Boehringer Ingelheim International Gmbh
Boehringer Ingelheim Pharma Gmbh & Co. Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim International Gmbh, Boehringer Ingelheim Pharma Gmbh & Co. Kg filed Critical Boehringer Ingelheim International Gmbh
Priority to JP2010530056A priority Critical patent/JP5497650B2/en
Priority to CA2702469A priority patent/CA2702469A1/en
Priority to EP08839335A priority patent/EP2215065B1/en
Priority to US12/738,087 priority patent/US8299123B2/en
Publication of WO2009052078A1 publication Critical patent/WO2009052078A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D249/00—Heterocyclic compounds containing five-membered rings having three nitrogen atoms as the only ring hetero atoms
    • C07D249/02—Heterocyclic compounds containing five-membered rings having three nitrogen atoms as the only ring hetero atoms not condensed with other rings
    • C07D249/04—1,2,3-Triazoles; Hydrogenated 1,2,3-triazoles
    • C07D249/06—1,2,3-Triazoles; Hydrogenated 1,2,3-triazoles with aryl radicals directly attached to ring atoms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/06—Antiasthmatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/04—Antipruritics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/06—Antipsoriatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/08—Antiallergic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C235/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms
    • C07C235/42—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton
    • C07C235/44—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring
    • C07C235/58—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring with carbon atoms of carboxamide groups and singly-bound oxygen atoms, bound in ortho-position to carbon atoms of the same non-condensed six-membered aromatic ring
    • C07C235/60—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring with carbon atoms of carboxamide groups and singly-bound oxygen atoms, bound in ortho-position to carbon atoms of the same non-condensed six-membered aromatic ring having the nitrogen atoms of the carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C235/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms
    • C07C235/42—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton
    • C07C235/44—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring
    • C07C235/58—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring with carbon atoms of carboxamide groups and singly-bound oxygen atoms, bound in ortho-position to carbon atoms of the same non-condensed six-membered aromatic ring
    • C07C235/62—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring with carbon atoms of carboxamide groups and singly-bound oxygen atoms, bound in ortho-position to carbon atoms of the same non-condensed six-membered aromatic ring having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a ring other than a six-membered aromatic ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/26—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D257/00—Heterocyclic compounds containing rings having four nitrogen atoms as the only ring hetero atoms
    • C07D257/02—Heterocyclic compounds containing rings having four nitrogen atoms as the only ring hetero atoms not condensed with other rings
    • C07D257/04—Five-membered rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2601/00—Systems containing only non-condensed rings
    • C07C2601/02—Systems containing only non-condensed rings with a three-membered ring

Definitions

  • This invention relates to substituted amides that are useful as inhibitors of CCRlO activity and are thus useful for treating a variety of diseases and disorders that are mediated or sustained through the activity of CCRlO including inflammatory skin diseases, allergic asthma and melanoma.
  • This invention also relates to pharmaceutical compositions comprising these compounds, methods of using these compounds in the treatment of various diseases and disorders, processes for preparing these compounds and intermediates useful in these processes.
  • Chemokine receptors play an important role in mediating tissue specific recruitment of leukocytes to sites of inflammation.
  • memory T cells that preferentially homes to the skin. This subset is defined by expression of the cutaneous lymphocyte antigen (CLA), a lectin, which binds to E-selectin on dermal endothelial cells and promotes trafficking.
  • CLA cutaneous lymphocyte antigen
  • the subset of CLA-expressing cells constitutes only 10- 15% of the circulating T cell pool, these cells are found in abundance within several inflammatory skin lesions, for example, psoriasis, contact sensitivity and allergic dermatitis.
  • CLA + memory cells also express the chemokine receptor CCRlO and that cells expressing CCRlO are enriched in inflammatory skin lesions.
  • CCL27 One ligand for this receptor, CCL27, is also markedly up-regulated at these sites suggesting that this chemokine receptor may participate in mediating the tissue-specific trafficking of CLA + memory T cells.
  • CCL27 expression has been shown to be tightly regulated with abundant expression in the epidermis, predominantly by keratinocytes.
  • endothelial cells within the vasculature of these lesions also display CCL27, though they are negative for CCL27 message, suggesting that keratinocyte- derived CCL27 can be captured by endothelial cells and presented to circulating leukocytes.
  • keratinocyte- derived CCL27 can be captured by endothelial cells and presented to circulating leukocytes.
  • IL- 1 beta and TNF alpha treatment of cultured keratinocytes induces expression of CCL27.
  • CCRlO antagonism of the interaction between CCRlO and its skin derived ligand CCL27 could therefore be of benefit in the treatment of inflammatory skin diseases by blocking the entry and activation of T cells within the skin.
  • One indication for a CCRlO antagonist would be psoriasis.
  • the rationale is based on histological studies of receptor/ligand expression in humans with psoriasis and proof of concept studies in animal models of skin inflammation. From analysis of normal and diseased skin samples, it is clear that the expression of CCRlO is highly regulated and restricted primarily to a subset of skin homing (CLA + ) lymphocytes, dermal endothelial cells, and dermal fibroblasts.
  • CLA + skin homing
  • CCL27 a ligand for CCRlO
  • CCL27 is also expressed in keratinocytes.
  • CCL27 is expressed by keratinocytes in the basal layers of the epidermis.
  • this ligand is up- regulated with expression extending to the suprabasal layers of the epidermis and histological staining also evident on the dermal microvasculature.
  • the enhanced expression of CCL27 is accompanied by an increased presence of CCRlO + lymphocytes.
  • CCRlO may also be a promising target for treatment of contact sensitivity and allergic dermatitis. It has been shown recently that CCL27 is increased in the sera of patients with systemic sclerosis (Hayakawa et al, Rheumatol, 2005, 44: 873) and in the dermis of UV-induced cutaneous SLE (systemic lupus erythematosus) lesions (Meller et al 2005, Arthritis Rheum 52: 1504). Therefore, systemic sclerosis and cutaneous SLE could also be additional indications.
  • Antagonism of CCRlO may also be beneficial for the treatment of melanoma.
  • melanoma lines expressing CCRlO form tumors more readily than matched CCRlO deficient melanomas and that a blocking antibody against CCL27 can block the growth of these CCRlO + melanoma cells in vivo.
  • the present invention is directed to the compounds of the following formula (I):
  • the present invention is directed to a method of inhibiting CCRlO activity in an individual comprising administering to the individual a compound described above.
  • the present invention is directed to a method of treating a disease or disorder associated with the activation of CCRlO comprising administering to an individual a compound described above.
  • the present invention is directed to a method of treating an inflammatory skin disease comprising administering to an individual a compound described above.
  • diseases include, for example, psoriasis, contact sensitivity, allergic dermatitis, systemic sclerosis, and cutaneous SLE.
  • the present invention is directed to a method of treating allergic asthma comprising administering to an individual a compound described above.
  • the present invention is directed to a method of treating melanoma comprising administering to an individual in need of such treatment a compound of the present invention as described above.
  • the present invention is directed to pharmaceutical compositions comprising the above-mentioned compounds, processes for preparing the above-mentioned compounds and intermediates used in these processes.
  • W, X, and Z are independently C or N;
  • Y is O, NH, or S
  • n 0 or 1 ;
  • Ci -8 alkyl branched or unbranched, optionally partially or fully halogenated, and optionally substituted with one to two groups selected from -OH, CN, Ci- galkoxy, -CO 2 Ci_ 6 alkyl, and -CON(Ci_ 3 alkyl)(Ci_ 3 alkyl),
  • R 1 may form a fused dihydropyran ring with the O it is bound to and X, said dihydropyran ring optionally substituted with one or two methyl groups;
  • Ci_ 6 alkyl (a) H, Ci_ 6 alkyl, Ci_ 6 alkoxy, hydroxyCi_ 6 alkyl, halogen, -CN, -CO 2 Ci_ 6 alkyl,
  • Ci_ 6 alkyl -NO 2 , -OH, -CF 3 , -NH 2 , -NH(Ci_ 6 alkyl), -N(Ci_ 6 alkyl)(Ci_ 6alkyl), -NHC(O)NHCi_ 6 alkyl, -C(O)NH 2 , -CONH(Ci_ 6 alkyl), -C0N(Ci_ 6alkyl)(C 1 _ 6 alkyl), or
  • phenyl, pyridinyl, triazolyl, or pyrimidinyl each optionally substituted with one or two groups selected from halogen, Ci_ 6 alkyl, -CN or Ci_ 6 alkoxy;
  • R 3 is H, -CO 2 H, -(CH 2 ) ⁇ 4 CO 2 H, -(CH 2 ) 0 -iC(Ci_6alkyl)(Ci_ 6 alkyl)CO 2 H, -C(Ci_ 6 alkyl)(Ci_ 6 - alkyl)CO 2 H, -0(CH 2 ) ⁇ CO 2 H, -O(CH 2 ) 0 -iC(Ci_6alkyl)(Ci_ 6 alkyl)CO 2 H, -OC(Ci_ 6 alkyl)(Ci_ 6 - alkyl)CO 2 H, -(CH 2 ) 0 -i-tetrazol-5-yl, -C(Ci_ 6 alkyl)(Ci_ 6 alkyl)-tetrazol-5-yl, -0-CH(Ci_ 6 alkyl)-tetrazol-5-yl, -C(O)NHCH 2 CO 2 H, or -
  • R 4 , R 5 , R 6 , and R 7 are independently selected from H and C ⁇ alkyl, or R 4 and R 6 may be joined, together with the carbons they are bonded to, to form a cyclopropane ring;
  • R 8 , R 9 , R 10 , and R 11 are independently H, halogen, C 1-6 a]kyl, Ci_ 6 alkoxy, -CN, -CO 2 Ci_ 6 alkyl, -C(O)NH 2 , -SO 2 NH 2 , -NO 2 , -OH, -NH 2 , -CF 3 , or -CH 2 OH;
  • W, X, and Z is C
  • Y is O
  • n 1;
  • Ci_ 8 alkyl branched or unbranched, optionally partially or fully halogenated, and optionally substituted with one to two groups selected from -OH, CN, Ci_ galkoxy, -CO 2 Ci_ 6 alkyl, -CON(Ci_ 3 alkyl)(Ci_ 3 alkyl),
  • Ar is phenyl or heteroaryl selected from pyridinyl, triazolyl, pyrimidinyl, furanyl, thiazolyl, thienyl, pyrrolyl, imidazolyl, and benzofuranyl, each optionally substituted with one to two groups selected from halogen, -CN, -OH, Ci_ 6 alkyl, Ci_ 6 alkoxy, -CO 2 Ci -6 alkyl, -C(O)NH 2 ; or
  • R 1 may be a fused dihydropyran ring with the O it is bound to and X, said dihydropyran ring optionally substituted with one or two methyl groups;
  • R 3 may be in the 4-position and is H, -CO 2 H, -(CH 2 ) ⁇ 4 CO 2 H, -(CH 2 )o-iC(C 1 _ 6 alkyl)(C 1 _ 6 _alkyl)C0 2 H, -C(C 1 . 6 alkyl)(C 1 .
  • R 3 may be in the 3 -position and is H or CO 2 H
  • R 4 , R 5 , R 6 , and R 7 are independently H or methyl, or R 4 and R 6 may be joined, together with the carbons they are bonded to, to form a cyclopropane ring;
  • R 8 , R 9 , R 10 , and R 11 are independently H, halogen, Ci_ 6 alkyl, Ci_ 6 alkoxy, -CN, -CO 2 Ci -6 alkyl, -C(O)NH 2 , -SO 2 NH 2 , -NO 2 , -OH, -NH 2 , -CF 3 , or -CH 2 OH;
  • Y is O
  • n 1;
  • Ci_ 8 alkyl branched or unbranched, optionally partially or fully fluorinated, and optionally substituted with one to two groups selected from -OH, CN and -OCH 3 ,
  • R 1 may form a fused dihydropyran ring with the O it is bound to and X, said dihydropyran ring optionally substituted with one or two methyl groups;
  • R 3 may be in the 4-position and is H, -CO 2 H, -(CH 2 ) ⁇ 4 CO 2 H, -(CH 2 ) 0 -iC(CH 3 )(CH 3 )CO 2 H, -C(CH 3 )(CH 3 )CO 2 H, -(CH 2 )o-itetrazol-5-yl, or -C(CH 3 )(CH 3 )tetrazol-5-yl; or
  • R 3 may be in the 3-position and is H or CO 2 H;
  • R 4 , R , R , and R 7 are independently H or methyl, or R 4 and R may be joined, together with the carbons they are bonded to, to form a cyclopropane ring;
  • R 8 , R 9 , R 10 , and R 11 are independently H, F, Cl, CH 3 , -OCH 3 , -CN, -NO 2 , -NH 2 , or -CF 3 ;
  • Y is O
  • n 1;
  • Ci_ 8 alkyl branched or unbranched, optionally partially or fully fluorinated
  • R 3 is in the 4-position and is H, -CO 2 H, -(CH 2 ) L2 CO 2 H, -(CH 2 )o-iC(CH 3 )(CH 3 )C0 2 H, -C(CH 3 )(CH 3 )CO 2 H, -(CH 2 ) 0 _itetrazol-5-yl, or -C(CH 3 )(CH 3 )tetrazol-5-yl;
  • R 4 , R 5 , R 6 , and R 7 are independently H or methyl, or R 4 and R 6 may be joined, together with the carbons they are bonded to, to form a cyclopropane ring;
  • R 8 , R 9 , R 10 , and R 11 are independently H, F, Cl, CH 3 , -OCH 3 , -CN, -NO 2 , or -NH 2 ;
  • Some of the compounds of formula (I) can exist in more than one tautomeric form.
  • the invention includes methods using all such tautomers.
  • the compounds of the invention are only those which are contemplated to be chemically stable as will be appreciated by those skilled in the art.
  • a compound that would have a dangling valency or carbanion is not a compound contemplated by the present invention.
  • the invention includes pharmaceutically acceptable derivatives of compounds of formula (I).
  • a "pharmaceutically acceptable derivative” refers to any pharmaceutically acceptable salt or ester, or any other compound that, upon administration to a patient, is capable of providing (directly or indirectly) a compound useful for the invention, or a pharmacologically active metabolite, or pharmacologically active residue thereof.
  • a pharmacologically active metabolite shall be understood to mean any compound of the invention capable of being metabolized enzymatically or chemically. This includes, for example, hydroxylated or oxidized derivative compounds of the formula (I).
  • Pharmaceutically acceptable salts include those derived from pharmaceutically acceptable inorganic and organic acids and bases.
  • suitable acids include hydrochloric, hydrobromic, sulfuric, nitric, perchloric, fumaric, maleic, phosphoric, glycolic, lactic, salicylic, succinic, toluene-p- sulfuric, tartaric, acetic, citric, methanesulfonic, formic, benzoic, malonic, naphthalene-2-sulfuric, and benzenesulfonic acids.
  • Other acids, such as oxalic acid while not themselves pharmaceutically acceptable per se, may be employed in the preparation of salts useful as intermediates in obtaining the compounds and their pharmaceutically acceptable acid addition salts.
  • Salts derived from appropriate bases include alkali metal ⁇ e.g., sodium), alkaline earth metal ⁇ e.g., magnesium), ammonium, and N-(Cl- C4 alkyl)4+ salts.
  • prodrugs of compounds of the formula (I) include those compounds that, upon simple chemical transformation, are modified to produce compounds of the invention. Simple chemical transformations include hydrolysis, oxidation, and reduction. Specifically, when a prodrug is administered to a patient, the prodrug may be transformed into a compound disclosed hereinabove, thereby imparting the desired pharmacological effect.
  • the invention additionally provides for methods for making the compounds of the formula (I). Specific, exemplary procedures are provided in the Synthetic Examples section.
  • compounds of formula I may be prepared by coupling of a 2-alkoxybenzoicacid II with an amine III using EDC, DDC, or other coupling reagents.
  • the amide nitrogen may be optionally alkylated by reaction with an alkylating reagent (R 9 Y), such as an alkyl chloride, bromide, iodide, or triflate, in the presence of a suitable base.
  • R 9 Y alkylating reagent
  • the amine III may be prepared from a protected amine anlog IV, where P is a suitable protecting group, such as a t-Boc group, and an organometallic reagent V via cross coupling reactions catalyzed by a transition metal, such as palladium followed by deprotection as illustrated in Scheme II.
  • P is a suitable protecting group, such as a t-Boc group
  • organometallic reagent V via cross coupling reactions catalyzed by a transition metal, such as palladium followed by deprotection as illustrated in Scheme II.
  • compounds of formula I may be prepared according to Scheme III.
  • the benzoic acid derivative II may be coupled with an amine analog IV to provide VII, which may be further coupled with an organometallic reagent to provide compounds of formula I.
  • a 2- hydroxybenzoic acid analog IF may be coupled with an amine using standard methods such as EDC or DCC to produce compounds of formula F.
  • Compounds of formula F can be reacted with an alkylating reagent RiY, where Y may be a Cl, Br, I, or TfO, in the presence of a suitable base, to generate compounds of formula I.
  • Reagents and Conditions a) CbzCl, Et 3 N, CH 2 Cl 2 , -30 ⁇ 23 0 C, 5 h; b) PdCl 2 (dppf), 2 M aq. Na 2 CO 3 , DME, 85 0 C, 18 h; c) H 2 , 10% Pd-C, CH 2 Cl 2 -EtOH, 6 h; d) EDC, HOBt, /-Pr 2 NEt, DMF, 14 h; e) Alkyl bromide, Cs 2 CO 3 or K 2 CO 3 or NaHCO 3 (for 1° alkyl bromide), 80 0 C; f)TFA, CH 2 Cl 2 , 23 0 C, 5-15 h.
  • the crude product is purified by passing through a silica gel pad and eluting with 5% MeOH in CH 2 Cl 2 and then 1 :2 EtOAc-hexanes to give the desired intermediate 1.1 (7.5 g, 97%) as a white solid.
  • the crude product is triturated in 25 mL of 1 : 1 hexane- EtOAc to give the desired amide 1.5 (2.54 g, 56%).
  • the above amide 1.5 (70 mg, 0.16 mmol) is dissolved in 1 mL DMF. Pentyl bromide (100 mg, 0.66 mmol) is then added followed by CS 2 CO 3 (100 mg, 0.31 mmol). The mixture is stirred at 85°C for 14 h.
  • the reaction mixture is diluted with EtOAc and washed with water three times and dried over Na 2 SO 4 , and concentrated in vacuo to give the desired product 1.6 (76 mg, 94%).
  • the crude carboxylic acid (2.5 g) is dissolved in 200 mL EtOH without further purification and SOCI 2 (4.83 mL, 57.8 mmol, 1 eq.) is added drop-wise to the stirring solution at ambient temperature. The mixture is then heated to reflux and stirred for 6 h. The reaction mixture is concentrated under reduced pressure. The residue is dissolved in the 100 mL ether and washed with water, saturated aqueous NaHCO3, and saturated brine successively. The organic layer is dried over anhydrous MgSO4 and concentrated under reduced pressure after filtration. The crude product is purified with a combiflash column (20%-40% EtOAc/hexane, on 4Og column) to yield yellow oil as product 3.9 (14.0 g, 48.8 mmol, 84.4%).
  • Nitrile 8.28 (0.3 g, 1.0 mmol) is dissolved in MeOH and added to 50% Raney nickel and subjected to hydrogenation at 50 psi overnight in a Parr shaker. The reaction mixture is filtered through a short pad of diatomaceous earth and concentrated to dryness to give 0.20 g of crude 8.29 (67%) of as a dark yellow solid that is usable for the next reaction without purification.
  • aqueous acetonitrile mixture is extracted with diethyl ether (2 x 100 mL) and the combined ether extracts are dried over MgSO 4 , filtered and evaporated to dryness to give a yellow viscous oil (0.84 g, 99%).
  • 1 H NMR analysis indicates the product is a 3:1 mixture of the desired 4-bromo compound 10.36 and the 3,4-dibromo by-product. This mixture is taken to the next step without further purification.
  • N-vinylphthalimide (7.3 g, 42.1 mmol), benzyltriethylammonium chloride (0.20 g, 0.88 mmol), and rhodium diacetate dimer (38 mg, 0.086 mmol).
  • EtOAc 200 mL
  • the organic layer is separated, dried over Na 2 SO 4 , filtered and evaporated to dryness to give the crude solid that is purified by a flash silica gel chromatography (eluent: 10% EtOAc in hexanes) to give 11.41 (1.0 g, 40%) as a white solid.
  • ester 12.45 (900 mg, 2.87 mmol) in methanol (30 niL) is added 2 N NaOH (6 mL). After stirring overnight at room temperature, TLC analysis indicates complete consumption of the starting material. The solvent is removed at reduced pressure, and the residue is diluted with water and acidified to pH 2 with concentrated hydrochloric acid. The mixture is extracted with ethyl acetate (3 x 50 mL) and the combined extracts are washed with brine, dried over sodium sulfate, filtered, and concentrated at reduced pressure to afford
  • the filter cake is rinsed thoroughly with ethyl acetate, and the filtrate layers are separated.
  • the aqueous layer is extracted with ethyl acetate (2 x 30 mL), and the organic layers are combined, washed with brine, dried over sodium sulfate, filtered, and concentrated at reduced pressure.
  • the residue is flash chromatographed (silica gel, dichloromethane to 99:1 dichloromethane/10% cone, ammonium hydroxide in methanol) to afford 12.49 (41 mg, 25%) as a white solid.
  • EDC (83 mg, 0.43 mmol), HOBt (60 mg, 0.44 mmol), Hunig's base (86 mg, 0.66 mmol) and DMAP (3 mg, 0.024 mmol) are added to a stirred solution of amine 49 (68 mg, 0.22 mmol) and 2-butoxy-5-chlorobenoic acid (86 mg, 0.38 mmol) in DMF (2 mL). After stirring overnight at room temperature, ethyl acetate and water are added. The layers are separated, and the aqueous layer is extracted with ethyl acetate (2 x 20 mL).
  • Reagents and Conditions a) MeNO 2 , NH 4 Ac, HOAc, 110 0 C, 3 h; b) LiBH 4 , TMSCl, 23 0 C, 24 h; c) EDC, HOBt, /-Pr 2 NEt, DMF, 15 h; d) PdCl 2 (dppf)CH 2 Cl 2 , 2 M aq. Na 2 CO 3 , DME, 85 0 C, 14 h; e) NaOH, THF-MeOH, 70 0 C, 2 h. (795-025).
  • the crude ester product is passed through a short silica column to give 17.70 as yellowish oil which is used for the saponification step without further purification.
  • 17.70 122 mg, 0.219 mmol
  • 1 mL of THF-EtOH 1: 1 v/v
  • aq. NaOH 2 M, 219 microL, 0.438 mmol
  • the mixture is stirred at room temperature overnight.
  • the reaction mixture is acidified with 3 N HCl to pH 2-3 and diluted with 1.5 mL of water-MeCN-DMSO (1:1 :1).
  • the crude product is obtained by filtration and is purified by reverse phase HPLC to provide the title compound 4 as a colorless oil (69 mg, 60% in two steps).
  • Preferred compounds have an IC 50 of 500 nM or lower in this assay.
  • CHO-Kl hCCRlO cells (Euroscreen cat # ES-143-A) are diluted in media to a final concentration of 2.8 X 10 5 cells/mL and 25 microL of this suspension are added to each well of a BD384 well TC treated assay plate (VWR #62406-490). This will yield approximately 7,000 cells/well. The plate is incubated at 37°C/5% CO 2 overnight.
  • the EC50 and EC70 should be calculated each time the assay is performed.
  • CTACK/CCL27 R&D Systems # 376-CT; 30 microM stock
  • peptide buffer HBSS/lmM CaCl/lmM MgSO 4 /0.1% BSA
  • This is serially dilute 1:3 in the same buffer for a total of 11 concentrations of peptide.
  • the assay below is run and the EC50 of the CCRlO peptide is calculated. Test compounds are assayed at the EC70.
  • Plate reader data are analyzed using ActivityBase software (ID Business Solutions, Ltd).
  • BCTRL is the average of background (negative control) well signals on the plate
  • PCTRL is the average of positive control well signals on the plate.
  • A, B, C, and D are fitted parameters (parameter B is fixed at zero POC), and x and y are the independent and dependent variables, respectively.
  • the IC 50 (50% inhibitory concentration) is determined as the inflection point parameter, C.
  • Compound powders are diluted in vials to 10 mM in 100% DMSO. 9.7 microL 10 mM stock are added to 80 microL 100 % DMSO in column 1 of a 96 well plate. 60 microL 100% DMSO are added to remaining wells. The compound is serially dilute compound by adding 30 microL column 1 to 60 microL in column 2. Column 2 is mixed dilution is continued across the 96 well plate ending at column 10. This step is done on the Beckman FX with no tip change between columns. 16 compounds or 2x 96 well plates can be tested in Ix 384 well plate. Each 96 well plate is transferred to 1 X384 well plate. Columns 21 through 24 on the 384 well plate contain DMSO vehicle control only.
  • Ix HBSS (1Ox, Invitrogen #14185-027), 10 mM HEPES pH 7.4, 0.35 g/L sodium bicarbonate, 1 mM CaCl 2 , 1 mM MgSO 4
  • Test compounds are evaluated for their ability to inhibit chemotaxis of Baf/3 cells expressing human CCRlO (hereinafter Baf/3-hCCRlO cells) in response to CCL27.
  • Preferred compounds have IC50 ⁇ 1 micromolar in this assay.
  • Test compounds are diluted (2x the final concentration) in CTX media (RPMI 1640 (Gibco- BRL #11875-093) supplemented with 0.1% BSA (Sigma #A3803)).
  • Control solutions contain 1% DMSO in CTX media.
  • Baf/3-hCCRlO cells are re-suspended in CTX media to a concentration of 4xlO 6 cells/mL.
  • 100 microL the Baf/3-hCCR10 cell suspension is combined with 100 microL of the test compound solution and the plate is then incubated for 15 min at room temperature.
  • a solution of the chemoattractant (2x the EC70 for CCL27) in CTX media is added to appropriate wells of a 96- well chemotaxis chamber (Neuro Probe Cat. #:116-5, 5um pore size, 5.7mm diameter size, 300 microL, 96 well plate).
  • CTX media without chemoattractant is added to control wells.
  • 152 microL of 2x compound solution in CTX media is added to appropriate wells.
  • the chamber is assembled according to manufacturer's instructions using the 5 micron pore size PVP-free polycarbonate filter. Care should be taken to avoid bubbles as they will cause variation.
  • the chamber is then disassembled and the filter is removed.
  • 150 microL of media is gently removed from each well of the chemotaxis chamber.
  • the remaining 150 microL is then mixed and 100 microL of the resulting cell suspension is transferred into a 96 well Costar 3917assay plate (Corning incorporated, cat # 3917).
  • the cells are measured using a CyQU ANT® NF Cell Proliferation Assay (Invitrogen, cat # C35006).
  • 11 niL of Ix HBSS buffer is prepared by diluting 2.2 mL of 5x HBSS buffer (Component C) with 8.8 mL of deionized water.
  • Ix dye binding solution is prepared by adding 22 microL of CyQU ANT® NF dye reagent (Component A) and 22 microL of Component C to 11 mL of Ix HBSS buffer. 100 microL of Ix dye binding solution is dispensed into wells of the 96 well Costar plate containing the cell suspensions. The plate is covered and incubated at 37 0 C for 60 minutes. Fluorescence measurement is quantitated using a multilabel plate reader (Wallac Victor2).
  • Wallac Victor2 multilabel plate reader
  • novel methods of using the compounds of the present invention effectively block the interaction of CCRlO with its ligand CCL 27.
  • the inhibition of this interaction is an attractive means for preventing and treating a variety of diseases or conditions associated with entry and activation of T-cells into the skin or other tissues where CCRlO is found to be expressed and associated with inflammatory conditions, such as lung tissue.
  • the compounds of the present invention are useful for the treatment of diseases and conditions including psoriasis, contact sensitivity, dermatitis, systemic sclerosis, cutaneous systemic lupus erythematosus, and allergic asthma.
  • the compounds of the invention will also be useful for treatment of melanomas that express CCRlO.
  • the compounds may be administered in any conventional dosage form in any conventional manner.
  • Routes of administration include, but are not limited to, intravenously, intramuscularly, subcutaneously, intrasynovially, by infusion, sublingually, transdermally, orally, topically or by inhalation, tablet, capsule, caplet, liquid, solution, suspension, emulsion, lozenges, syrup, reconstitutable powder, granule, suppository and transdermal patch.
  • Methods for preparing such dosage forms are known (see, for example, H.C. Ansel and N. G. Popovish, Pharmaceutical Dosage Forms and Drug Delivery Systems, 5th ed., Lea and Febiger (1990)).
  • the compounds may be administered alone or in combination with adjuvants that enhance the stability of the inhibitors, facilitate administration of pharmaceutical compositions containing them in certain embodiments, provide increased dissolution or dispersion, increase inhibitory activity, provide adjunct therapy, and the like.
  • adjuvants for use with compounds according to the present invention include, for example, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, buffer substances, water, salts or electrolytes and cellulose-based substances.

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Abstract

The invention relates to a compound of formula (I) or a tautomer thereof or a pharmaceutically acceptable salt thereof, wherein R1 to R11, W, X, Y, Z, and n are as defined herein. The invention also relates to methods of using the compounds of formula (I) and compositions thereof to treat various diseases and disorders in a patient. The invention also relates to processes for preparing the compounds of formula (I) and intermediates useful in these processes.

Description

CCRlO ANTAGONISTS
Background of the Invention
Field of the Invention
This invention relates to substituted amides that are useful as inhibitors of CCRlO activity and are thus useful for treating a variety of diseases and disorders that are mediated or sustained through the activity of CCRlO including inflammatory skin diseases, allergic asthma and melanoma. This invention also relates to pharmaceutical compositions comprising these compounds, methods of using these compounds in the treatment of various diseases and disorders, processes for preparing these compounds and intermediates useful in these processes.
Brief Description of the Art
Chemokine receptors play an important role in mediating tissue specific recruitment of leukocytes to sites of inflammation. Within the blood there is a subset of memory T cells that preferentially homes to the skin. This subset is defined by expression of the cutaneous lymphocyte antigen (CLA), a lectin, which binds to E-selectin on dermal endothelial cells and promotes trafficking. Although the subset of CLA-expressing cells constitutes only 10- 15% of the circulating T cell pool, these cells are found in abundance within several inflammatory skin lesions, for example, psoriasis, contact sensitivity and allergic dermatitis. Recent studies have revealed that CLA+ memory cells also express the chemokine receptor CCRlO and that cells expressing CCRlO are enriched in inflammatory skin lesions. One ligand for this receptor, CCL27, is also markedly up-regulated at these sites suggesting that this chemokine receptor may participate in mediating the tissue-specific trafficking of CLA+ memory T cells. Within the skin, expression of CCRlO has been reported on CLA+ T cells, melanocytes, fibroblasts, and microvascular endothelial cells. CCL27 expression has been shown to be tightly regulated with abundant expression in the epidermis, predominantly by keratinocytes.
There is evidence in both humans and in rodents that the CCR10-CCL27 interaction plays an important role in the trafficking of inflammatory T cell subsets to skin lesions (J. Morales et al, Proc Natl Acad Sci USA, 1999, 96: 14470-14475; B. Homey et al, J Immunol 2000; 164: 3465-3470; B. Homey et al, Nature Medicine, 2002; 8: 157-165). By histological analysis, it is clear that, in addition to the increase in epidermal expression of CCL27 observed in psoriatic and atopic dermatitis biopsies, there is also expanded expression of CCL27 into the dermal layer as well. Further, endothelial cells within the vasculature of these lesions also display CCL27, though they are negative for CCL27 message, suggesting that keratinocyte- derived CCL27 can be captured by endothelial cells and presented to circulating leukocytes. Accompanying these changes in the skin is a marked increase in the recruitment of lymphocytes that co-express CLA and CCRlO. Consistent with the role of CCL27 in skin inflammation, IL- 1 beta and TNF alpha treatment of cultured keratinocytes induces expression of CCL27.
Cutaneous application of nickel, in nickel-allergic humans, led to the up-regulated expression of CCL27 and the subsequent recruitment of CCRlO+ lymphocytes. Thus, these studies provide temporal support for the role of CCL27 in attracting CCRlO+ cells. Furthermore, in vivo proof of concept has been shown (B. Homey et al, ibid, 2002) in wild- type mice where treatment with a function blocking antibody against CCL27 clearly diminished recruitment and swelling in both DNFB-induced and ovalbumin DTH models of dermatitis. These authors also demonstrated the ability of cutaneous injection of CCL27 to promote local lymphocyte trafficking and inflammation, thus providing proof of concept using both ligand and antibody in relevant animal models. Consistent with its ascribed in vivo role, CCL27 induces calcium flux in CCRlO+ cells and mediates the selective chemotaxis of CLA+ CCRlO+ lymphocytes in vitro.
Studies, such as those described above, suggest that antagonism of the interaction between CCRlO and its skin derived ligand CCL27 could therefore be of benefit in the treatment of inflammatory skin diseases by blocking the entry and activation of T cells within the skin. One indication for a CCRlO antagonist would be psoriasis. The rationale is based on histological studies of receptor/ligand expression in humans with psoriasis and proof of concept studies in animal models of skin inflammation. From analysis of normal and diseased skin samples, it is clear that the expression of CCRlO is highly regulated and restricted primarily to a subset of skin homing (CLA+) lymphocytes, dermal endothelial cells, and dermal fibroblasts. In addition, CCL27, a ligand for CCRlO, is also expressed in keratinocytes. In normal skin, CCL27 is expressed by keratinocytes in the basal layers of the epidermis. However, in the skin of atopic dermatitis and psoriasis patients this ligand is up- regulated with expression extending to the suprabasal layers of the epidermis and histological staining also evident on the dermal microvasculature. The enhanced expression of CCL27 is accompanied by an increased presence of CCRlO+ lymphocytes. Finally the proof of concept studies described above demonstrated that a function blocking antibody directed against CCL27 blocked trafficking of lymphocytes and swelling in two murine models of dermatitis.
Based on the pattern of expression for both CCRlO and CCL27 and the above proof of concept studies, CCRlO may also be a promising target for treatment of contact sensitivity and allergic dermatitis. It has been shown recently that CCL27 is increased in the sera of patients with systemic sclerosis (Hayakawa et al, Rheumatol, 2005, 44: 873) and in the dermis of UV-induced cutaneous SLE (systemic lupus erythematosus) lesions (Meller et al 2005, Arthritis Rheum 52: 1504). Therefore, systemic sclerosis and cutaneous SLE could also be additional indications. In addition, inflammation of the respiratory tract in a murine model of allergic asthma is associated with CCL28 and CCRlO expression suggesting that inhibition of CCRlO activity may also be useful in treatment of allergic asthma (English et al, Immunol Lett. 2006, 03(2) :92- 100).
Antagonism of CCRlO may also be beneficial for the treatment of melanoma. In a mouse model of melanoma metastasis, it has been demonstrated that melanoma lines expressing CCRlO form tumors more readily than matched CCRlO deficient melanomas and that a blocking antibody against CCL27 can block the growth of these CCRlO+ melanoma cells in vivo. These observations, coupled with the finding that many human melanomas express CCRlO, provide the rationale for considering this as a further indication (Murakami et al, J Exp Med, 2003, 198: 1337). Brief Summary of the Invention
In a general aspect, the present invention is directed to the compounds of the following formula (I):
Figure imgf000007_0001
(I) wherein R > 1 - τR» l l , W, X, Y, Z, and n are as defined herein, as well as the tautomers thereof, and salts thereof. It has been found that the compounds of formula (I) have valuable pharmacological properties, particularly an inhibiting activity of CCRlO activity.
In another aspect, the present invention is directed to a method of inhibiting CCRlO activity in an individual comprising administering to the individual a compound described above.
In another aspect, the present invention is directed to a method of treating a disease or disorder associated with the activation of CCRlO comprising administering to an individual a compound described above.
In another aspect, the present invention is directed to a method of treating an inflammatory skin disease comprising administering to an individual a compound described above. Examples of such diseases that may be treated include, for example, psoriasis, contact sensitivity, allergic dermatitis, systemic sclerosis, and cutaneous SLE.
In another aspect, the present invention is directed to a method of treating allergic asthma comprising administering to an individual a compound described above.
In another aspect, the present invention is directed to a method of treating melanoma comprising administering to an individual in need of such treatment a compound of the present invention as described above.
In yet additional aspects, the present invention is directed to pharmaceutical compositions comprising the above-mentioned compounds, processes for preparing the above-mentioned compounds and intermediates used in these processes.
Detailed Description of the Invention
In an embodiment, there are provided compounds of the formula (I)
Figure imgf000008_0001
(I) wherein:
W, X, and Z are independently C or N;
Y is O, NH, or S;
n is 0 or 1 ;
R1 is
(a) H;
(b) Ci-8alkyl, branched or unbranched, optionally partially or fully halogenated, and optionally substituted with one to two groups selected from -OH, CN, Ci- galkoxy, -CO2Ci_6alkyl, and -CON(Ci_3alkyl)(Ci_3alkyl),
(c) -(CH2)o-iC3-8cycloalkyl,
(d) -CH2Ar, wherein Ar is phenyl or heteroaryl selected from pyridinyl, triazolyl, pyrimidinyl, furanyl, thiazolyl, thienyl, pyrrolyl, imidazolyl, and benzofuranyl, each optionally substituted with one to two groups selected from halogen, -CN, -OH, Ci_6alkyl, Ci_6alkoxy, -CF3, -CO2Ci_6alkyl, and -CONH2, or (e) -(CH2)2θCH2Ar, wherein Ar is phenyl or heteroaryl selected from pyridinyl, triazolyl, pyrimidinyl, furanyl, thiazolyl, thienyl, pyrrolyl, imidazolyl, and benzofuranyl, each optionally substituted with one to two groups selected from halogen, -CN, -OH, C^alkyl, d_6alkoxy, -C02d_6alkyl, -C(O)NH2; or
if X is C, and Y is O, R1 may form a fused dihydropyran ring with the O it is bound to and X, said dihydropyran ring optionally substituted with one or two methyl groups;
R2 is
(a) H, Ci_6alkyl, Ci_6alkoxy, hydroxyCi_6alkyl, halogen, -CN, -CO2Ci_6alkyl,
-S(0)o-2Ci_6alkyl, -NO2, -OH, -CF3, -NH2, -NH(Ci_6alkyl), -N(Ci_6alkyl)(Ci_ 6alkyl), -NHC(O)NHCi_6alkyl, -C(O)NH2, -CONH(Ci_6alkyl), -C0N(Ci_ 6alkyl)(C1_6alkyl), or
b) phenyl, pyridinyl, triazolyl, or pyrimidinyl, each optionally substituted with one or two groups selected from halogen, Ci_6alkyl, -CN or Ci_6alkoxy;
R3 is H, -CO2H, -(CH2)^4CO2H, -(CH2)0-iC(Ci_6alkyl)(Ci_6alkyl)CO2H, -C(Ci_6alkyl)(Ci_6- alkyl)CO2H, -0(CH2)^CO2H, -O(CH2)0-iC(Ci_6alkyl)(Ci_6alkyl)CO2H, -OC(Ci_6alkyl)(Ci_6- alkyl)CO2H, -(CH2)0-i-tetrazol-5-yl, -C(Ci_6alkyl)(Ci_6alkyl)-tetrazol-5-yl, -0-CH(Ci_ 6alkyl)-tetrazol-5-yl, -C(O)NHCH2CO2H, or -CN;
R4, R5, R6, and R7 are independently selected from H and C^alkyl, or R4 and R6 may be joined, together with the carbons they are bonded to, to form a cyclopropane ring; R8, R9, R10, and R11 are independently H, halogen, C1-6a]kyl, Ci_6alkoxy, -CN, -CO2Ci_6alkyl, -C(O)NH2, -SO2NH2, -NO2, -OH, -NH2, -CF3, or -CH2OH;
or a tautomer thereof or a salt thereof.
In another embodiment there are provided compounds of formula (I) as described above and wherein:
W, X, and Z is C;
Y is O;
n is 1;
R1 is
(a) H,
(b) Ci_8alkyl, branched or unbranched, optionally partially or fully halogenated, and optionally substituted with one to two groups selected from -OH, CN, Ci_ galkoxy, -CO2Ci_6alkyl, -CON(Ci_3alkyl)(Ci_3alkyl),
(c) -(CH2)o-iC3.8cycloalkyl, (d) -CH2Ar, wherein Ar is phenyl or heteroaryl selected from pyridinyl, triazolyl, pyrimidinyl, furanyl, thiazolyl, thienyl, pyrrolyl, imidazolyl, and benzofuranyl, each optionally substituted with one to two groups selected from halogen, - CN, -OH, C^alkyl, C1-6alkoxy, -CF3, -CO2C1_6alkyl, and -CONH2, or
(e) -(CH2^OCH2Ar wherein Ar is phenyl or heteroaryl selected from pyridinyl, triazolyl, pyrimidinyl, furanyl, thiazolyl, thienyl, pyrrolyl, imidazolyl, and benzofuranyl, each optionally substituted with one to two groups selected from halogen, -CN, -OH, Ci_6alkyl, Ci_6alkoxy, -CO2Ci-6alkyl, -C(O)NH2; or
R1 may be a fused dihydropyran ring with the O it is bound to and X, said dihydropyran ring optionally substituted with one or two methyl groups;
R2 is
(a) H, Ci-βalkyl, Ci-βalkoxy, hydroxyCi_6alkyl, halogen, -CN, -CO2Ci_6alkyl, -S(O)0-2Ci_6alkyl, -NO2, -OH, -CF3, -NH2, -NH(C1-6alkyl), -N(Ci_6alkyl)(Ci_ galkyl), -NHC(O)NHC1-6alkyl, -C(O)NH2, -CONH(Ci_6alkyl), -C0N(Ci_ 6alkyl)(Ci_6alkyl), or
(b) phenyl, pyridinyl, triazolyl and pyrimidinyl, each optionally substituted with one or two groups selected from halogen, Ci_6alkyl, -CN or Ci_6alkoxy;
R3 may be in the 4-position and is H, -CO2H, -(CH2)^4CO2H, -(CH2)o-iC(C1_6alkyl)(C1_6_alkyl)C02H, -C(C1.6alkyl)(C1.6alkyl)CO2H, -C(O)NH2, -(CH2)O-1- tetrazol-5-yl, -C(Ci-6alkyl)(Ci_6alkyl)-tetrazol-5-yl, -O-CH(Ci_6alkyl)-tetrazol-5-yl, -C(O)NHCH2CO2H, or -CN; or
R3 may be in the 3 -position and is H or CO2H
R4, R5, R6, and R7 are independently H or methyl, or R4 and R6 may be joined, together with the carbons they are bonded to, to form a cyclopropane ring;
R8, R9, R10, and R11 are independently H, halogen, Ci_6alkyl, Ci_6alkoxy, -CN, -CO2Ci-6alkyl, -C(O)NH2, -SO2NH2, -NO2, -OH, -NH2, -CF3, or -CH2OH;
or a tautomer thereof or a salt thereof.
In yet another embodiment, there are provided compounds of formula (I) as described above and wherein:
W, X, and Z is C:
Y is O;
n is 1;
RMs
(a) H, (b) Ci_8alkyl, branched or unbranched, optionally partially or fully fluorinated, and optionally substituted with one to two groups selected from -OH, CN and -OCH3,
(c) -(CH2)o-iC3-8cycloalkyl, or
(d) -CH2Ar, wherein Ar is phenyl or heteroaryl selected from pyridinyl and thiazolyl, each optionally substituted with one to two groups selected from halogen, -CN, -CH3, -OCH3, -CF3, and -CONH2; or
R1 may form a fused dihydropyran ring with the O it is bound to and X, said dihydropyran ring optionally substituted with one or two methyl groups;
R2 is
(a) -Cl, -Br, -CN, -CO2Ci_6alkyl, -NO2, -OH, -CF3, -NH2; or
(b) phenyl, pyridinyl, or pyrimidinyl;
R3 may be in the 4-position and is H, -CO2H, -(CH2)^4CO2H, -(CH2)0-iC(CH3)(CH3)CO2H, -C(CH3)(CH3)CO2H, -(CH2)o-itetrazol-5-yl, or -C(CH3)(CH3)tetrazol-5-yl; or
R3 may be in the 3-position and is H or CO2H; R4, R , R , and R7 are independently H or methyl, or R4 and R may be joined, together with the carbons they are bonded to, to form a cyclopropane ring;
R8, R9, R10, and R11 are independently H, F, Cl, CH3, -OCH3, -CN, -NO2, -NH2, or -CF3;
or a tautomer thereof or a salt thereof.
In a further embodiment, there are provided compounds of formula (I) as described above and wherein:
W, X, and Z are C:
Y is O;
n is 1;
R1 is
(a) Ci_8alkyl, branched or unbranched, optionally partially or fully fluorinated;
(b) -(CH2)o-iC3_8cycloalkyl, or
(c) -CH2Ar, wherein Ar is phenyl or heteroaryl selected from pyridinyl and thiazolyl, each optionally substituted with one to two groups selected from F, CN, -CH3, -OCH3, and -CF3, R is Cl or Br;
R3 is in the 4-position and is H, -CO2H, -(CH2)L2CO2H, -(CH2)o-iC(CH3)(CH3)C02H, -C(CH3)(CH3)CO2H, -(CH2)0_itetrazol-5-yl, or -C(CH3)(CH3)tetrazol-5-yl;
R4, R5, R6, and R7 are independently H or methyl, or R4 and R6 may be joined, together with the carbons they are bonded to, to form a cyclopropane ring;
R8, R9, R10, and R11 are independently H, F, Cl, CH3, -OCH3, -CN, -NO2, or -NH2;
or a tautomer thereof or a salt thereof.
In still a further embodiment of the invention, there are provided compounds of the formula (I) selected from the group below or a tautomer thereof or a salt thereof:
Structure Name
1 . (4'-{2-[(2-butoxy-5- chlorobenzoyl) amino] ethyl } biphenyl-
4-yl)acetic acid
2. 2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -2,5- difluorobiphenyl-4-yl)oxy] -2- methylpropanoic acid
Figure imgf000016_0001
3. 4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -3- chlorobiphenyl-4-carboxylic acid
Figure imgf000017_0001
2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -3- fluorobiphenyl-4-yl)oxy] -2-
Figure imgf000017_0002
methylpropanoic acid
2-[(4'-{2-[(5-chloro-2- methoxybenzoyl) amino] ethyl } -2 , 3 - dimethylbiphenyl-4-yl)oxy] -2-
Figure imgf000017_0003
methylpropanoic acid
2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl) amino] ethyl } biphenyl- 4-yl)oxy]-2-methylpropanoic acid
Figure imgf000017_0004
4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -2- nitrobiphenyl-4-carboxylic acid
Figure imgf000017_0005
8. 2-{ [4'-(2-{ [2-(benzyloxy)-5- chlorobenzoyl] amino } ethyl)biphenyl- 4-yl]oxy}-2-methylpropanoic acid
Figure imgf000018_0001
9. 2-(benzyloxy)-5-chloro-N-{2-[4'-(lH- tetrazol-5-yl)biphenyl-4- yl] ethyl } benzamide
Figure imgf000018_0002
10. 2-[(4'-{2-[(5-chloro-2- methoxybenzoyl) amino] ethyl } -2 , 5 - difluorobiphenyl-4-yl)oxy] -2-
Figure imgf000018_0003
methylpropanoic acid
1 1 . 4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -2- chlorobiphenyl-4-carboxylic acid
Figure imgf000018_0004
12. 2-[(4'-{2-[(5-chloro-2- propoxybenzoyl) amino] ethyl } biphenyl- 4-yl)oxy]-2-methylpropanoic acid
Figure imgf000018_0005
13. 4'-[2-({5-chloro-2-[(3- fluorobenzyl)oxy] benzoyl } amino)ethyl ]biphenyl-4-carboxylic acid
Figure imgf000019_0001
14. 4'-[2-({5-chloro-2-[(3- methoxybenzyl)oxy] benzoyl } amino)et hyl]biphenyl-4-carboxylic acid
Figure imgf000019_0002
15. 2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -2- fluorobiphenyl-4-yl)oxy] -2-
Figure imgf000019_0003
methylpropanoic acid
16. 2-butoxy-5 -chloro-N-(2- { 4'- [ 1 -methyl- l-(lH-tetrazol-5-yl)ethyl]biphenyl-4- yl } ethyl)benzamide
Figure imgf000019_0004
17. 4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -3- fluorobiphenyl-4-carboxylic acid
Figure imgf000019_0005
Figure imgf000020_0001
23. 4'-(2-{ [5-chloro-2-
(heptyloxy)benzoyl] amino } ethyl)biphe nyl-4-carboxylic acid
Figure imgf000021_0001
24. 4'-[2-({5-chloro-2-[(4- methylpentyl)oxy] benzoyl } amino)ethyl ]biphenyl-4-carboxylic acid
25. 2-[(2,3-dichloro-4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}biphenyl -4-yl)oxy] -2-methylpropanoic acid
Figure imgf000021_0002
26. 2-butoxy-5 -chloro-N- { 2- [4'-( 1 H- tetrazol-5 -ylmethyl)biphenyl-4- yl]ethyl Jbenzamide
Figure imgf000021_0003
27. 4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -2- methylbiphenyl-4-carboxylic acid
Figure imgf000021_0004
28. 2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -3- methylbiphenyl-4-yl)oxy] -2-
Figure imgf000021_0005
methylpropanoic acid 29. 2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -2- methylbiphenyl-4-yl)oxy] -2-
Figure imgf000022_0001
methylpropanoic acid
30. 2-[(2-chloro-4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}biphenyl -4-yl)oxy] -2-methylpropanoic acid
Figure imgf000022_0002
31 . 4'-[2-({5-chloro-2-[(3- methylbenzyl)oxy]benzoyl } amino)ethy l]biphenyl-4-carboxylic acid
32. 2-[(4'-{2-[(5-chloro-2- methoxybenzoyl)amino] ethyl } -2- methylbiphenyl-4-yl)oxy] -2-
Figure imgf000022_0003
methylpropanoic acid
33. 2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -2,3- dimethylbiphenyl-4-yl)oxy] -2-
Figure imgf000022_0004
methylpropanoic acid 34. 2-[(4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}-3- methylbiphenyl-4-yl)oxy ] -2-
Figure imgf000023_0001
methylpropanoic acid
35. 4'-[2-({5-chloro-2-[(4- methylbenzyl)oxy]benzoyl } amino)ethy l]biphenyl-4-carboxylic acid
Figure imgf000023_0002
36. 4'-[2-({5-chloro-2-[(6- hydroxyhexyl)oxy]benzoyl } amino)ethy l]biphenyl-4-carboxylic acid
Figure imgf000023_0003
37. 2-[(4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl } -2- fluorobiphenyl-4-yl)oxy] -2-
Figure imgf000023_0004
methylpropanoic acid
38. 2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -2- chlorobiphenyl-4-yl)oxy] -2-
Figure imgf000023_0005
methylpropanoic acid 39. 2-[(4'-{2-[(5-chloro-2- isopropoxybenzoyl)amino]ethyl}biphe nyl-4-yl)oxy] -2-methylpropanoic acid
Figure imgf000024_0001
40. 4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -3- methoxybiphenyl-4-carboxylic acid
Figure imgf000024_0002
41 . 2-{ [4'-(2-{ [5-chloro-2-(3,3,4,4,4- pentafluorobutoxy)benzoyl] amino } ethy l)biphenyl-4-yl] oxy } -2- methylpropanoic acid
Figure imgf000024_0003
42. 2-[(4'-{2-[(5-chloro-2- methoxybenzoyl) amino] ethyl } -2 , 5 - dimethylbiphenyl-4-yl)oxy] -2-
Figure imgf000024_0004
methylpropanoic acid
43. 4'- [2-( { 2- [2-(benzyloxy)ethoxy] -5 - chlorobenzoyl } amino)ethyl]biphenyl- 4-carboxylic acid
Figure imgf000024_0005
Figure imgf000025_0001
49. 2-[(4'-{2-[(5-chloro-2- ethoxybenzoyl)amino] ethyl } biphenyl- 4-yl)oxy]-2-methylpropanoic acid
Figure imgf000026_0001
50. 4'-(2-{ [5-chloro-2-(4,4,4- trifluorobutoxy)benzoyl] amino } ethyl) - biphenyl-4-carboxylic acid
Figure imgf000026_0002
51 . 3-(4'-{2-[(2-butoxy-5- chlorobenzoyl) amino] ethyl } biphenyl- 4-yl)propanoic acid
Figure imgf000026_0003
52. 4'-{2-[(2-butoxy-5- chlorobenzoyl) amino] ethyl } -5 - nitrobiphenyl-3-carboxylic acid
Figure imgf000026_0004
53. 2-(4'-{2-[(2-butoxy-5- chlorobenzoyl) amino] ethyl } biphenyl- 4-yl)-2-methylpropanoic acid
Figure imgf000026_0005
54. 2-[(4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl } -3 '- fluorobiphenyl-4-yl)oxy] -2-
Figure imgf000027_0001
methylpropanoic acid
55. 4'-[2-({5-chloro-2-[(4- fluorobenzyl)oxy] benzoyl } amino)ethyl ]biphenyl-4-carboxylic acid
Figure imgf000027_0002
56. 4'-[2-({5-chloro-2-[(3,4- difluorobenzyl)oxy]benzoyl } amino)eth yl]biphenyl-4-carboxylic acid
Figure imgf000027_0003
57. (4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -3- nitrobiphenyl-4-yl)acetic acid
Figure imgf000027_0004
58. 4'-(2-{ [5-chloro-2-(3- methylbutoxy)benzoyl] amino } ethyl)bip henyl-4-carboxylic acid
Figure imgf000027_0005
59. 4'-{2-[(2-butoxy-5- chlorobenzoyl) amino] ethyl } biphenyl-
3-carboxylic acid
60. 4'-[2-({5-chloro-2-[(2- fluorobenzyl)oxy] benzoyl } amino)ethyl ]biphenyl-4-carboxylic acid
61 . 4'-{2-[(5-chloro-2-{ [3- (trifluoromethyl)benzyl]oxy}benzoyl)a mino]ethyl}biphenyl-4-carboxylic acid
62. 4'-(2-{ [5-chloro-2-
(cyclobutylmethoxy)benzoyl] amino } et hyl)biphenyl-4-carboxylic acid
63. 4'-(2-{ [(5-bromo-2,2-dimethyl-2,3- dihydro- 1 -benzofuran-7- yl)carbonyl] amino }ethyl)biphenyl-4-
Figure imgf000028_0001
carboxylic acid
64. 5-chloro-N-[2-(2'-cyanobiphenyl-4- yl)ethyl] -2-hydroxybenzamide
Figure imgf000028_0002
65. 3-(4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}biphenyl -4-yl)-2,2-dimethylpropanoic acid
Figure imgf000029_0001
66. 2-chloro-4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}biphenyl -4-carboxylic acid
Figure imgf000029_0002
67. 2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -3- cyanobiphenyl-4-yl)oxy] -2-
Figure imgf000029_0003
methylpropanoic acid
68. 4'-[2-({5-chloro-2-[(3- cyanobenzyl)oxy]benzoyl } amino)ethyl ]biphenyl-4-carboxylic acid
69. 4'-(2-{ [5-chloro-2-
(cyclohexylmethoxy)benzoyl] amino } et hyl)biphenyl-4-carboxylic acid
Figure imgf000029_0004
70. 4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -6- fluorobiphenyl-3-carboxylic acid
Figure imgf000030_0001
71 . 4'-{2-[(2-butoxy-5- chlorobenzoyl)amino] - 1 -methyl-ethyl } - biphenyl-4-carboxylic acid
Figure imgf000030_0002
72. 4'-(2-{ [5-chloro-2-(3,3,4,4,4- pentafluorobutoxy)benzoyl] amino } ethy l)biphenyl-4-carboxylic acid
Figure imgf000030_0003
73. 4'-(2-{ [(2-butoxy-5-chloropyridin-3- yl)carbonyl] amino } ethyl)biphenyl-4- carboxylic acid
Figure imgf000030_0004
74. 2-[(4'-{2-[(5-chloro-2- methoxybenzoyl) amino] ethyl } biphenyl -4-yl)oxy] -2-methylpropanoic acid
Figure imgf000030_0005
75. 2-butoxy-5 -chloro-N-(2- { 4- [6-( 1 H- tetrazol-5 -yl)pyridin-3 - yl] phenyl } ethyl)benzamide
Figure imgf000031_0001
76. 4'-(2-{ [5-chloro-2-(pyridin-2- ylmethoxy)benzoyl] amino } ethyl)biphe nyl-4-carboxylic acid
Figure imgf000031_0002
77. 4'-{2-[(5-chloro-2-{ [2- (trifluoromethyl)benzyl]oxy}benzoyl)a mino]ethyl}biphenyl-4-carboxylic acid
Figure imgf000031_0003
78. 2-[(3-chloro-4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}biphenyl -4-yl)oxy] -2-methylpropanoic acid
Figure imgf000031_0004
79. 4'-{2-[(2-butoxy-5- chlorobenzoyl)amino] ethyl } -2- cyanobiphenyl-4-carboxylic acid
Figure imgf000031_0005
80. 4'-{2-[(5-chloro-2- ethoxybenzoyl)amino]ethyl}biphenyl- 4-carboxylic acid
81 . 4'-(2-{ [5-chloro-2-(l- methylbutoxy)benzoyl] amino } ethyl)bip henyl-4-carboxylic acid
Figure imgf000032_0001
82. 3-chloro-4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}biphenyl -4-carboxylic acid
Figure imgf000032_0002
83. 2-[(2',6'-dichloro-4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}biphenyl
Figure imgf000032_0003
-4-yl)oxy] -2-methylpropanoic acid
84. 4'-{2-[(5-chloro-2- isobutoxybenzoyl)amino]ethyl}bipheny 1-4-carboxylic acid
Figure imgf000032_0004
85. 4'-{2-[(5-chloro-2- propoxybenzoyl)amino]ethyl}biphenyl- 4-carboxylic acid
Figure imgf000033_0001
86. 4'-[2-({5-chloro-2-[(4-methyl-l,3- thiazol-2- yl)methoxy] benzoyl } amino)ethyl]biphe
Figure imgf000033_0002
nyl-4-carboxylic acid
87. 2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -2,5- dimethylbiphenyl-4-yl)oxy] -2-
Figure imgf000033_0003
methylpropanoic acid
88. 4'-(2-{ [5-chloro-2-(4- fluorobutoxy)benzoyl] amino } ethyl)bip henyl-4-carboxylic acid
Figure imgf000033_0004
89. 2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -3- chlorobiphenyl-4-yl)oxy] -2-
Figure imgf000034_0001
methylpropanoic acid
90. 4'-{2-[(5-chloro-2- hydroxybenzoyl)amino]ethyl}biphenyl-
2-carboxamide
Figure imgf000034_0002
91 . (4'-{2-[(5-chloro-2- methoxybenzoyl) amino] ethyl } biphenyl
-4-yl)acetic acid
Figure imgf000034_0003
92. 4'-(2-{ [5-chloro-2-
(cyclopropylmethoxy)benzoyl] amino } e thyl)biphenyl-4-carboxylic acid
Figure imgf000034_0004
93. 4'-(2-{ [5-chloro-2-(pyridin-3- ylmethoxy)benzoyl] amino } ethyl)biphe nyl-4-carboxylic acid
Figure imgf000034_0005
Figure imgf000035_0001
99. 2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -2,6- dimethylbiphenyl-4-yl)oxy] -2-
Figure imgf000036_0001
methylpropanoic acid
100 2-(4'-{2-[(5-chloro-2- methoxybenzoyl) amino] ethyl } biphenyl -4-yl)-2-methylpropanoic acid
Figure imgf000036_0002
101 4'-{2-[(2-butoxy-5-pyridin-3- ylbenzoyl)amino]ethyl}biphenyl-4- carboxylic acid
Figure imgf000036_0003
102. 4'-(2-{ [5-chloro-2-
(cyclobutyloxy)benzoyl] amino } ethyl)- biphenyl-4-carboxylic acid
Figure imgf000036_0004
103. 2',6'-dichloro-4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}biphenyl -4-carboxylic acid
Figure imgf000036_0005
104. 4'-{2-[(2-butoxy-5-pyrimidin-5- ylbenzoyl)amino]ethyl}biphenyl-4- carboxylic acid
Figure imgf000037_0001
105 2-({4'-[N-(2-butoxy-5- chlorobenzoyl)glycyl]biphenyl-4- yl}oxy)-2-methylpropanoic acid
106 5 -amino-4'- { 2- [(2-butoxy-5 - chlorobenzoyl) amino] ethyl } biphenyl- 3-carboxylic acid
Figure imgf000037_0002
107 2-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino]ethyl } -2,3- dichlorobiphenyl-4-yl)oxy] -2-
Figure imgf000037_0003
methylpropanoic acid
108 4'-{(lR,2S)-2-[(2-butoxy-5- chlorobenzoyl)amino] cyclopropyl } - biphenyl-4-carboxylic acid
Figure imgf000037_0004
109. 4'-(2-{ [(4-butoxybiphenyl-3- yl)carbonyl] amino } ethyl)biphenyl-4- carboxylic acid
Figure imgf000038_0001
1 10 4'-(2-{ [5-chloro-2-
(cyclopentyloxy)benzoyl] amino } ethyl) biphenyl-4-carboxylic acid
Figure imgf000038_0002
1 1 1 4'-(2-{ [5-chloro-2-
(cycloheptyloxy)benzoyl] amino } ethyl) biphenyl-4-carboxylic acid
Figure imgf000038_0003
1 12. (4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl } -3- nitrobiphenyl-4-yl)acetic acid
1 13. 5-chloro-N-[2-(4'-cyanobiphenyl-4- yl)ethyl] -2-hydroxybenzamide
Figure imgf000038_0004
14. 4'-{(lS,2S)-2-[(2-butoxy-5- chlorobenzoyl)amino]cyclopropyl } - biphenyl-4-carboxylic acid
Figure imgf000039_0001
15. 4'-(3-{ [2-(benzyloxy)-5- chlorophenyl] amino } -3 - oxopropyl)biphenyl-4-carboxylic acid
16. 4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}-3- fluorobiphenyl-4-carboxylic acid
Figure imgf000039_0002
17 5-chloro-2-hydroxy-N-[2-(4-pyridin-3- ylphenyl)ethyl]benzamide
Figure imgf000039_0003
18 2-[(4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}biphenyl -3-yl)oxy]-2-methylpropanoic acid
Figure imgf000039_0004
1 19. 4'-(2-{ [2-(butylamino)-5- chlorobenzoyl] amino } ethyl)biphenyl- 4-carboxylic acid
Figure imgf000040_0001
120. 4'-(2-{ [2-butoxy-5-(l-methyl-lH-l,2,3- triazol-5- yl)benzoyl] amino } ethyl)biphenyl-4- carboxylic acid
Figure imgf000040_0002
121 5-chloro-2-hydroxy-N-{2-[4'-(lH- tetrazol-5-yl)biphenyl-4- yl] ethyl } benzamide
Figure imgf000040_0003
122 5-(4-{2-[(2-butoxy-5- chlorobenzoy 1) amino ] ethyl } phenyl) - nicotinic acid
Figure imgf000040_0004
123. 4'-(2-{ [2-(benzyloxy)-5- chlorobenzoyl] amino } ethyl)biphenyl- 2-carboxamide
Figure imgf000040_0005
124. 4'-[2-({5-chloro-2-[(2- methylbenzyl)oxy]benzoyl } amino)- ethyl]biphenyl-4-carboxylic acid
Figure imgf000041_0001
125. 4'-(2-{ [5-chloro-2-(2- phenylethoxy)benzoyl] amino } ethyl) - biphenyl-4-carboxylic acid
Figure imgf000041_0002
126 2-[(4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl } -T- methoxybiphenyl-4-yl)oxy] -2- methylpropanoic acid
Figure imgf000041_0003
127 4'-{2-[(5-chloro-2- isopropoxybenzoyl)amino]ethyl}- biphenyl-4-carboxylic acid
Figure imgf000041_0004
128 2-[(4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}-3- methoxybiphenyl-4-yl)oxy] -2-
Figure imgf000041_0005
methylpropanoic acid 129. 2-[(4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}-3- cyanobiphenyl-4-yl)oxy] -2-
Figure imgf000042_0001
methylpropanoic acid
130. 4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl } -2- cyanobiphenyl-4-carboxylic acid
Figure imgf000042_0002
131 . 4'-{2-[(2-butoxy-5-pyrimidin-2- ylbenzoyl)amino]ethyl}biphenyl-4- carboxylic acid
Figure imgf000042_0003
132. 2-butoxy-5-chloro-N-methyl-N-(2-{4'- [l-(lH-tetrazol-5-yl)ethoxy]biphenyl- 4-yl } ethyl)benzamide
Figure imgf000042_0004
133. 4'-{3-[(2-butoxy-5- chlorophenyl)amino] -3 - oxopropyl }biphenyl-4-carboxylic acid
Figure imgf000042_0005
Figure imgf000043_0001
139. 4'- [2-( { 2- [(3-carbamoylbenzyl)oxy] -5 - chlorobenzoyl } amino)ethyl]biphenyl- 4-carboxylic acid
Figure imgf000044_0001
140. 4'-(2-{ [5-chloro-2-
(cyclohexyloxy)benzoyl] amino } ethyl)- biphenyl-4-carboxylic acid
Figure imgf000044_0002
141 . tert-butyl 4'-(2-{ [2-methoxy-4- (morpholin-4- ylcarbonyl)benzoyl] amino } ethyl)- biphenyl-4-carboxylate
Figure imgf000044_0003
142. 2-[(4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}-2,6- dimethylbiphenyl-4-yl)oxy] -2-
Figure imgf000044_0004
methylpropanoic acid
143. N-[(4'-{2-[(2-butoxy-5- chlorobenzoyl)amino] ethyl } biphenyl- 4-yl)carbonyl] glycine
Figure imgf000044_0005
144. 4'-[2-({5-chloro-2-[(2,6- dichlorobenzyl)oxy ] benzoyl } amino) - ethyl]biphenyl-4-carboxylic acid
Figure imgf000045_0001
145. 4'-{2-[(5-bromo-2-hydroxy-3- methylbenzoyl)amino] ethyl } biphenyl- 4-carboxylic acid
Figure imgf000045_0002
146. 4'-(2-{ [2-(butylthio)-5- chlorobenzoyl] amino } ethyl)biphenyl- 4-carboxylic acid
Figure imgf000045_0003
147. 4'-(2-{ [5-chloro-2-(3,3,3- trifluoropropoxy)benzoyl] amino } ethyl) biphenyl-4-carboxylic acid
Figure imgf000045_0004
148. 4'-({ [(2-butoxy-5- chlorophenyl)carbamoyl] amino } methyl )biphenyl-4-carboxylic acid
Figure imgf000045_0005
149. 4'-{2-[(2-butoxy-5- chlorobenzoyl) amino] ethyl } biphenyl-
2-carboxamide
Figure imgf000046_0001
150. 4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}biphenyl
-2-carboxamide
Figure imgf000046_0002
151 . N- { 2-[4'-(2-amino- 1 , 1 -dimethyl-2- oxoethoxy)biphenyl-4-yl]ethyl}-2- butoxy- 5 -chlorobenzamide
Figure imgf000046_0003
152. 2-({4'-[N-(5-chloro-2- methoxybenzoyl)glycyl]biphenyl-4-
Figure imgf000046_0004
yl}oxy)-2-methylpropanoic acid
153. 4'-{2-[(5-chloro-2- methoxybenzoyl)amino]ethyl}-3- methoxybiphenyl-4-carboxylic acid
Figure imgf000046_0005
154. 5-chloro-2-methoxy-N-[2-(4-pyridin-3- ylphenyl)ethyl]benzamide
Figure imgf000046_0006
Figure imgf000047_0001
In an additional embodiment of the invention there are provided compounds of the formula (I) selected from the group below or a tautomer thereof or a salt thereof:
(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl }biphenyl-4-yl)acetic acid;
2-[(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2,5-difluorobiphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-3-chlorobiphenyl-4-carboxylic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -3 -fluorobiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2-[(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}-2,3-dimethylbiphenyl-4-yl)oxy]-2- methylpropanoic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl }biphenyl-4-yl)oxy] -2-methylpropanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2-nitrobiphenyl-4-carboxylic acid;
2-{ [4'-(2-{ [2-(benzyloxy)-5-chlorobenzoyl]amino}ethyl)biphenyl-4-yl]oxy}-2- methylpropanoic acid;
2-(benzyloxy)-5-chloro-N-{2-[4'-(lH-tetrazol-5-yl)biphenyl-4-yl]ethyl}benzamide; 2-[(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}-2,5-difluorobiphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2-chlorobiphenyl-4-carboxylic acid;
2-[(4'-{2-[(5-chloro-2-propoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2-methylpropanoic acid;
4'-[2-({5-chloro-2-[(3-fluorobenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
4'-[2-({5-chloro-2-[(3-methoxybenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -2-fluorobiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2-butoxy-5-chloro-N-(2-{4'-[l-methyl-l-(lH-tetrazol-5-yl)ethyl]biphenyl-4- yl } ethyl)benzamide ;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-3-fluorobiphenyl-4-carboxylic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -3 -methoxybiphenyl-4-yl)oxy] -2- methylpropanoic acid;
4'-(2-{ [5-chloro-2-(hexyloxy)benzoyl] amino }ethyl)biphenyl-4-carboxylic acid; 2-[(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-3-yl)oxy]-2-methylpropanoic acid;
2-{ [4'-(2-{ [5-chloro-2-(4,4,4-trifluorobutoxy)benzoyl]amino}ethyl)biphenyl-4-yl]oxy}-2- methylpropanoic acid;
4'-(2-{ [5-chloro-2-(pentyloxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid;
4'-(2-{ [5-chloro-2-(heptyloxy)benzoyl] amino }ethyl)biphenyl-4-carboxylic acid;
4'-[2-({5-chloro-2-[(4-methylpentyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
2-[(2,3-dichloro-4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2- methylpropanoic acid;
2-butoxy-5-chloro-N-{2-[4'-(lH-tetrazol-5-ylmethyl)biphenyl-4-yl]ethyl}benzamide;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2-methylbiphenyl-4-carboxylic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -3 -methylbiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -2-methylbiphenyl-4-yl)oxy] -2- methylpropanoic acid; 2-[(2-chloro-4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-[2-({5-chloro-2-[(3-methylbenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
2- [(4'- { 2- [(5 -chloro-2-methoxybenzoyl)amino] ethyl } -2-methylbiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2-[(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2,3-dimethylbiphenyl-4-yl)oxy]-2- methylpropanoic acid;
2-[(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}-3-methylbiphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-[2-({5-chloro-2-[(4-methylbenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
4'-[2-({5-chloro-2-[(6-hydroxyhexyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
2- [(4'- { 2- [(5 -chloro-2-methoxybenzoyl)amino] ethyl } -2-fluorobiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -2-chlorobiphenyl-4-yl)oxy] -2- methylpropanoic acid; 2-[(4'-{2-[(5-chloro-2-isopropoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-3-methoxybiphenyl-4-carboxylic acid;
2-{ [4'-(2-{ [5-chloro-2-(3,3,4,4,4-pentafluorobutoxy)benzoyl]amino}ethyl)biphenyl-4- yl]oxy}-2-methylpropanoic acid;
2-[(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}-2,5-dimethylbiphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-[2-({2-[2-(benzyloxy)ethoxy]-5-chlorobenzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
2-{ [4'-(2-{ [5-chloro-2-(4-fluorobutoxy)benzoyl]amino}ethyl)biphenyl-4-yl]oxy}-2- methylpropanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-5-fluorobiphenyl-3-carboxylic acid
2-[(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}-3-fluorobiphenyl-4-yl)oxy]-2- methylpropanoic acid;
3-(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-4-yl)-2,2-dimethylpropanoic acid;
4'-{2-[(5-bromo-2-butoxybenzoyl)amino]ethyl}biphenyl-4-carboxylic acid; 2- [(4'- { 2- [(5 -chloro-2-ethoxybenzoyl)amino] ethyl } biphenyl-4-yl)oxy] -2-methylpropanoic acid;
4'-(2-{ [5-chloro-2-(4,4,4-trifluorobutoxy)benzoyl] amino }ethyl)biphenyl-4-carboxylic acid;
3 -(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl }biphenyl-4-yl)propanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-5-nitrobiphenyl-3-carboxylic acid;
2-(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-4-yl)-2-methylpropanoic acid;
2-[(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}-3'-fluorobiphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-[2-({5-chloro-2-[(4-fluorobenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
4'-[2-({5-chloro-2-[(3,4-difluorobenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-3-nitrobiphenyl-4-yl)acetic acid;
4'-(2-{ [5-chloro-2-(3-methylbutoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-3-carboxylic acid; 4'-[2-({5-chloro-2-[(2-fluorobenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
4'- { 2- [(5 -chloro-2- { [3 -(trifluoromethyl)benzyl] oxy }benzoyl)amino] ethyl } biphenyl-4- carboxylic acid;
4'-(2-{ [5-chloro-2-(cyclobutylmethoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid;
4'-(2-{ [(5-bromo-2,2-dimethyl-2,3-dihydro-l-benzofuran-7-yl)carbonyl]amino}ethyl)- biphenyl-4-carboxylic acid;
5-chloro-N-[2-(2'-cyanobiphenyl-4-yl)ethyl]-2-hydroxybenzamide;
3-(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)-2,2-dimethylpropanoic acid;
2-chloro-4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-carboxylic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -3 -cyanobiphenyl-4-yl)oxy] -2- methylpropanoic acid;
4'-[2-({5-chloro-2-[(3-cyanobenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
4'-(2-{ [5-chloro-2-(cyclohexylmethoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid; 4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-6-fluorobiphenyl-3-carboxylic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]-l-methylethyl}biphenyl-4-carboxylic acid;
4'-(2-{ [5-chloro-2-(3,3,4,4,4-pentafluorobutoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid;
4'-(2-{ [(2-butoxy-5-chloropyridin-3-yl)carbonyl]amino}ethyl)biphenyl-4-carboxylic acid;
2-[(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2-methylpropanoic acid;
2-butoxy-5-chloro-N-(2-{4-[6-(lH-tetrazol-5-yl)pyridin-3-yl]phenyl}ethyl)benzamide;
4'-(2-{ [5-chloro-2-(pyridin-2-ylmethoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid;
4'-{2-[(5-chloro-2-{ [2-(trifluoromethyl)benzyl]oxy}benzoyl)amino]ethyl}biphenyl-4- carboxylic acid;
2-[(3-chloro-4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2-cyanobiphenyl-4-carboxylic acid;
4'-{2-[(5-chloro-2-ethoxybenzoyl)amino]ethyl}biphenyl-4-carboxylic acid; 4'-(2-{ [5-chloro-2-(l-methylbutoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid;
3-chloro-4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-carboxylic acid;
2-[(2',6'-dichloro-4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-{2-[(5-chloro-2-isobutoxybenzoyl)amino]ethyl}biphenyl-4-carboxylic acid;
4'-{2-[(5-chloro-2-propoxybenzoyl)amino]ethyl}biphenyl-4-carboxylic acid;
4'-[2-({5-chloro-2-[(4-methyl-l,3-thiazol-2-yl)methoxy]benzoyl}amino)ethyl]biphenyl-4- carboxylic acid;
2-[(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2,5-dimethylbiphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-(2-{ [5-chloro-2-(4-fluorobutoxy)benzoyl] amino }ethyl)biphenyl-4-carboxylic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -3 -chlorobiphenyl-4-yl)oxy] -2- methylpropanoic acid;
4'-{2-[(5-chloro-2-hydroxybenzoyl)amino]ethyl}biphenyl-2-carboxamide; (4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)acetic acid;
4'-(2-{ [5-chloro-2-(cyclopropylmethoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid;
4'-(2-{ [5-chloro-2-(pyridin-3-ylmethoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]-l,l-dimethylethyl}biphenyl-4-carboxylic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]propyl}biphenyl-4-carboxylic acid;
4'-(2-{ [(5-bromo-2,3-dihydro-l-benzofuran-7-yl)carbonyl]amino}ethyl)biphenyl-4- carboxylic acid;
2-{ [4'-(2-{ [5-chloro-2-(3,3,3-trifluoropropoxy)benzoyl]amino}ethyl)biphenyl-4-yl]oxy}-2- methylpropanoic acid;
2-amino-4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-4-carboxylic acid;
2-[(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2,6-dimethylbiphenyl-4-yl)oxy]-2- methylpropanoic acid;
2-(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)-2-methylpropanoic acid;
4'-{2-[(2-butoxy-5-pyridin-3-ylbenzoyl)amino]ethyl}biphenyl-4-carboxylic acid; 4'-(2-{ [5-chloro-2-(cyclobutyloxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-4-carboxylic acid;
2-(4-{5-[2-(2-butoxy-5-chloro-benzoylamino)-ethyl]-pyridin-2-yl}-phenoxy)-2-methyl- propionic acid;
2-{4'-[2-(2-butoxy-5-chloro-benzoylamino)-ethyl]-3'-ethyl-biphenyl-4-yloxy}-2-methyl- propionic acid
2-{3',5'-Dichloro-4'-[2-(5-chloro-2-methoxy-benzoylamino)-ethyl]-biphenyl-4-yloxy}-2- methyl-propionic acid
In all the compounds disclosed in this application, in the event the nomenclature is in conflict with the structure, it shall be understood that the compound is defined by the structure.
Some of the compounds of formula (I) can exist in more than one tautomeric form. The invention includes methods using all such tautomers.
The compounds of the invention are only those which are contemplated to be chemically stable as will be appreciated by those skilled in the art. For example, a compound that would have a dangling valency or carbanion is not a compound contemplated by the present invention. The invention includes pharmaceutically acceptable derivatives of compounds of formula (I). A "pharmaceutically acceptable derivative" refers to any pharmaceutically acceptable salt or ester, or any other compound that, upon administration to a patient, is capable of providing (directly or indirectly) a compound useful for the invention, or a pharmacologically active metabolite, or pharmacologically active residue thereof. A pharmacologically active metabolite shall be understood to mean any compound of the invention capable of being metabolized enzymatically or chemically. This includes, for example, hydroxylated or oxidized derivative compounds of the formula (I).
Pharmaceutically acceptable salts include those derived from pharmaceutically acceptable inorganic and organic acids and bases. Examples of suitable acids include hydrochloric, hydrobromic, sulfuric, nitric, perchloric, fumaric, maleic, phosphoric, glycolic, lactic, salicylic, succinic, toluene-p- sulfuric, tartaric, acetic, citric, methanesulfonic, formic, benzoic, malonic, naphthalene-2-sulfuric, and benzenesulfonic acids. Other acids, such as oxalic acid, while not themselves pharmaceutically acceptable per se, may be employed in the preparation of salts useful as intermediates in obtaining the compounds and their pharmaceutically acceptable acid addition salts. Salts derived from appropriate bases include alkali metal {e.g., sodium), alkaline earth metal {e.g., magnesium), ammonium, and N-(Cl- C4 alkyl)4+ salts.
In addition, within the scope of the invention is use of prodrugs of compounds of the formula (I). Prodrugs include those compounds that, upon simple chemical transformation, are modified to produce compounds of the invention. Simple chemical transformations include hydrolysis, oxidation, and reduction. Specifically, when a prodrug is administered to a patient, the prodrug may be transformed into a compound disclosed hereinabove, thereby imparting the desired pharmacological effect.
General Synthetic Methods
The invention additionally provides for methods for making the compounds of the formula (I). Specific, exemplary procedures are provided in the Synthetic Examples section.
As illustrated in Scheme I, compounds of formula I may be prepared by coupling of a 2-alkoxybenzoicacid II with an amine III using EDC, DDC, or other coupling reagents. The amide nitrogen may be optionally alkylated by reaction with an alkylating reagent (R9Y), such as an alkyl chloride, bromide, iodide, or triflate, in the presence of a suitable base.
Figure imgf000060_0001
I (R9 =H)
Figure imgf000060_0002
Scheme I
The amine III may be prepared from a protected amine anlog IV, where P is a suitable protecting group, such as a t-Boc group, and an organometallic reagent V via cross coupling reactions catalyzed by a transition metal, such as palladium followed by deprotection as illustrated in Scheme II.
Figure imgf000061_0001
P = Protecting Group I = B, Sn, Zn etc Vl deprotection
Figure imgf000061_0002
Scheme II
Alternatively, compounds of formula I may be prepared according to Scheme III. The benzoic acid derivative II may be coupled with an amine analog IV to provide VII, which may be further coupled with an organometallic reagent to provide compounds of formula I.
Figure imgf000062_0001
VII
Figure imgf000062_0002
Scheme III
Another route useful in preparation of compounds of formula I is shown in Scheme IV. A 2- hydroxybenzoic acid analog IF may be coupled with an amine using standard methods such as EDC or DCC to produce compounds of formula F. Compounds of formula F can be reacted with an alkylating reagent RiY, where Y may be a Cl, Br, I, or TfO, in the presence of a suitable base, to generate compounds of formula I.
Figure imgf000063_0001
T(R9=H)
Figure imgf000063_0002
Scheme IV
Synthetic Examples
0.: Synthesis of 4'-(2-{ [5-chloro-2-(pentyloxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid (Compound 22)
Figure imgf000064_0001
Reagents and Conditions: a) CbzCl, Et3N, CH2Cl2, -30→23 0C, 5 h; b) PdCl2(dppf), 2 M aq. Na2CO3, DME, 85 0C, 18 h; c) H2, 10% Pd-C, CH2Cl2-EtOH, 6 h; d) EDC, HOBt, /-Pr2NEt, DMF, 14 h; e) Alkyl bromide, Cs2CO3 or K2CO3 or NaHCO3 (for 1° alkyl bromide), 800C; f)TFA, CH2Cl2, 23 0C, 5-15 h.
A solution of 4-bromophenylethyl amine (4.7 g, 23.5 mmol) and Et3N (6.5 mL, 47.0 mmol) in 100 mL of CH2Cl2 is cooled to -30 0C. Benzyl chloroformate (4 ml, 28.2 mmol) is added drop-wise. The reaction mixture is allowed to warm up to 23 0C slowly and stirred for 5 h. The reaction mixture is poured into crushed ice- water and extracted with CH2Cl2 (100 mL x 3). The organic layer is washed with water, brine, and dried over Na2SO4, and concentrated in vacuo. The crude product is purified by passing through a silica gel pad and eluting with 5% MeOH in CH2Cl2 and then 1 :2 EtOAc-hexanes to give the desired intermediate 1.1 (7.5 g, 97%) as a white solid.
To a solution of the bromide obtained above (5.5 g, 16.5 mmol) and the boronic acid 1.2 (7.3 g, 33 mmol) in 50 mL of DMF is added an aq. Na2CO3 solution (2 M, 22 niL, 44 mmol). The mixture is purged with Ar2 for 10 min. PdCl2(dppf) (672 mg, 0.82 mmol) is then added. The reaction is stirred under Ar2 at 85°C for 18 h. After cooling the reaction mixture is poured into water (200 mL). The mixture then is extracted with EtOAc (50 mLx 3). The organic layer is separated and dried over Na2SO4 and concentrated in vacuo. The crude product is purified by chromatography to provide the desired product 1.3 (5.5 g, 77%).
The above carbamate 1.3 is dissolved in CH2Cl2 (57 mL) and then EtOH (570 mL) is added, followed by 10% Pd/C (1. 5 g). The mixture is then stirred under 1 atmosphere H2 for 6 h. The reaction mixture is filtered through a pad of diatomaceous earth and the solid residues are rinsed with EtOH. The filtrate is concentrated in vacuo to give the desired amine 1.4 as a white solid (4.9 g, 100%).
A mixture of 5-chloro-2-hydroxybenzoic acid (2.1 g, 12.2 mmol), the above amine (3.0 g, 10.1 mmol), EDC (2.9 g, 15.1 mmol), HOBt (2.0 g, 15.0 mmol), Hunig's base (2.8 mL, 16.1 mmol) in 20 mL of DMF is stirred at 23 0C for 14 h. The reaction mixture is diluted with EtOAc (200 mL), washed with 1 M NaHSO4, sat. aq. NaHCO3, water, brine, dried over Na2SO4, and concentrated in vacuo. The crude product is triturated in 25 mL of 1 : 1 hexane- EtOAc to give the desired amide 1.5 (2.54 g, 56%). The above amide 1.5 (70 mg, 0.16 mmol) is dissolved in 1 mL DMF. Pentyl bromide (100 mg, 0.66 mmol) is then added followed by CS2CO3 (100 mg, 0.31 mmol). The mixture is stirred at 85°C for 14 h. The reaction mixture is diluted with EtOAc and washed with water three times and dried over Na2SO4, and concentrated in vacuo to give the desired product 1.6 (76 mg, 94%).
The above ester 1.6 (55 mg, 0.11 mmol) is dissolved in 2 mL of CH2Cl2. TFA (0.5 ml) is then added and the mixture is stirred at 23 0C for 5 h. The solvents are removed in vacuo, the residue is dissolved in 2 mL of MeOH, and diluted with 10 mL of 0.1 M aq. NaOH. The mixture is then extracted with 20 mL of ether. The aqueous layer is separated and acidified with 3 N HCl to pH 2, and extracted with 50 mL of EtOAc. The EtOAc extract is washed with brine, dried over Na2SO4, and concentrated in vacuo to give the title compound 22 (48 mg, 98%).
Example 2: Synthesis of 4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-4- carboxylic acid (Compound 156)
Figure imgf000066_0001
Compound 156 is prepared from intermediate 2.5 and n-BuBr by the same procedure as the preparation of compound 7. Example 3: Synthesis of 2-{ [4'-(2-{ [5-chloro-2-(4,4,4- trifluorobutoxy)benzoyl] amino } ethyl)-biphenyl-4-yl] oxy } -2-methylpropanoic acid
(Compound 21)
Figure imgf000067_0001
4-Bromophenol (10 g, 57.8 mmol, 1 eq.) and l,l,l-trichloro-2-methyl-2-propanol hemihydrate (20.5 g, 115.6 mmol, 2 eq.) in 200 mL acetone is treated with solid NaOH (18.5 g, 462.4 mmol, 8 eq.) and the reaction mixture is stirred at ambient temperature overnight. The solvent is removed under reduced pressure and the resulting residue is dissolved in 10 mL water. The resulting solution is acidified with 3 N HCl and extracted with ether. The extracts are washed twice with brine and dried over anhydrous MgSO4. The filtered solvent is removed under reduced pressure to give 2.5 g crude product. The crude carboxylic acid (2.5 g) is dissolved in 200 mL EtOH without further purification and SOCI2 (4.83 mL, 57.8 mmol, 1 eq.) is added drop-wise to the stirring solution at ambient temperature. The mixture is then heated to reflux and stirred for 6 h. The reaction mixture is concentrated under reduced pressure. The residue is dissolved in the 100 mL ether and washed with water, saturated aqueous NaHCO3, and saturated brine successively. The organic layer is dried over anhydrous MgSO4 and concentrated under reduced pressure after filtration. The crude product is purified with a combiflash column (20%-40% EtOAc/hexane, on 4Og column) to yield yellow oil as product 3.9 (14.0 g, 48.8 mmol, 84.4%).
A solution of 2-(4-bromo-phenoxy)-2-methyl-propionic acid ethyl ester (9, 35 mmol, 1 eq.), bis(pinacolato)diboron (Hg, 43 mmol, 1.2 eq.), and KOAc (7 g, 72 mmol, 2 eq.) in 100 mL anhydrous DMSO is purged with Argon for 10 min. The PdCl2(dppf) (1.5 g, 1.9 mmol, 0.1 eq.) catalyst is then added and the reaction mixture is sealed in a seal tube and stirred at 100 0C overnight. After cooling down, the reaction mixture is diluted with EtOAc and washed with water and brine. The organic layer is dried over Na2SO4, filtered and concentrated to give a dark oil that is purified on a combiflash column (40%— 60% EtOAc in hexane, 40 g column) and yields 10 (11 g, 33 mmol, 94%) as a white solid.
A solution of intermediate 3.3 (2g, 6 mmol, 1 eq.) and intermediate 3.10 (2g, 6 mmol, 1 eq.) in DMF is purged with Ar2 for 10 min. Pd(dppf)Cl2 (122 mg, 0.15 mmol, 0.4 eq.) and 8 mL 2N Na2Cθ3/H2O are then added and the resulting reaction mixture is sealed in a tube and heated to 120 0C in a microwave oven for 30 min. The reaction mixture is diluted with EtOAc and washed with water and brine. The organic layer is dried over Na2SO4, filtered and concentrated to give a dark oil that is purified by flash column (40%~60% EtOAc/hexanes, 40 g column) yielding intermediate 3.11 as a light greenish oil (2 g, 4.3 mmol, 72%).
Intermediate 3.11 (1.6 g, 3.5 mmol, 1 eq.) is dissolved in 150 mL of EtOH/DCM (10:1). 5% Pd/C is added to the solution and the resulting suspension is stirred under hydrogen for 3.12 hours. The reaction mixture is filtered through diatomaceous earth and washed with ethanol. After the solvent is evaporated under reduce pressure, intermediate 3.12 is obtained as white solid (1.13 g, 3.5 mmol, 99%).
A solution of ethyl amino-biphenyl-2-methyl-propionic acid ethyl ester (12, 690 mg, 2.1 mmol, 1 eq.) and 5-chloro-salicylic acid (727 mg, 4.2 mmol, 2 eq.), in 10 mL DMF is treated with EDC (802 mg, 4.2 mmol, 2 eq.), HOBT (569 mg, 4.2 mmol, 2 eq.) and DMAP (26 mg, 0.2 mmol, 0.2 eq.). After stirring at ambient temperature for 48 h, the reaction mixture is extracted between EtOAc and water. The organic layer is washed with saturated NaHCO3, saturated NaCl solution, and water. After drying over Na2SO4, the organic solvent is filtered and evaporated under reduce pressure. The crude product is loaded onto a Biotage column with 1 mL Of CH2Cl2. The column is eluted with 10% EtOAc/hexanes to yield 3.13 as a white solid (1.6 g, 3.5 mmol, 1 eq.).
Intermediate 3.13 (70 mg, 0.15 mmol, 1 eq.), in 1 mL DMF is treated with NaHCO3 (60 mg, 0.73 mmol, 5 eq.) and then 4-bromo-l,l,l-trifluoro-butane (88 mg, 0.45 mmol, 3 eq.) is added drop-wise. The reaction mixture is heated to 120 0C for 30 min by microwave. After cooling down to room temperature, 10 mL water is added and the reaction is extracted with 50 mL EtOAc and washed with water. After the organic layer is dried over NaSO4, filtered, and evaporated under reduced pressure, crude product is obtained. The crude product is loaded on the pre-TLC and developed with hexanes/EtOAc (3:1) to yield clear 3.14 as a thick oil (84 mg, 0.14 mmol, 97%).
A solution of 3.14 (40 mg, 0.07 mmol, 1 eq.), in 0.5 mL THF/ MeOH (1: 1) is treated with 70 μl 2N NaOH/H2O solution and the resulting reaction mixture is stirred at 50 0C for 2h. The solvent is evaporated and 1 mL water is added to the residue. The residue is acidified carefully with 1 N HCl and the resulting solution is extracted with EtOAc. The organic solvent is dried, filtered, and evaporated under reduced pressure to yield the title compound 21 as a white solid (36 mg, O.Oδmmol, 95%).
Example 4: Synthesis of 2-[(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-4- yl)oxy]-2-methylpropanoic acid (Compound 6)
Figure imgf000070_0001
Compound 6 is synthesized from 4.13 and n-BuBr using the same procedure as described for the preparation of intermediate 3.15 in the above Example.
Example 5: Synthesis of 4'-{2-[(5-bromo-2-butoxybenzoyl)amino]ethyl}-biphenyl-4- carboxylic acid (Compound 48)
Figure imgf000071_0001
To a solution of 5-bromosalicylic acid (300 mg, 1.24 mmol), amine 5.3 (359 mg, 1.21 mmol), and HOBT (202mg, 1.49 mmol) in DMF (10 mL) is added EDC (358mg, 1.87 mmol) followed by DMAP. The mixture is stirred at room temperature overnight. After removal of the solvent, the mixture is purified by silica gel chromatography using 0-40% ethyl acetate - hexane (gradient) to give 500 mg of product 5.17.
To a solution of 5.17 (lOOmg, 0.20 mmol) in DMF (2 mL) is add NaHCO3 (68 mg, 0.81 mmol) followed by n-butyl bromide (55 mg, 0.40 mmol). The mixture is heated at 60 0C for 4 hours, cooled to room temperature, and concentrated. Purification of the residue by silica gel chromatography using 0-40% ethyl acetate - hexane (gradient) gives 98 mg of intermediate 5.18.
To a solution of ester 5.18 (58 mg, 0.105 mmol) in dichloromethane (1.5 mL) is added trifluoroacetic acid (0.5 mL). The mixture is stirred at room temperature overnight. Removal of the solvent gives 48 mg of the title compound 48 as white solid. Example 6: Synthesis of 4'-{2-[(2-butoxy-5-pyrimidin-5-ylbenzoyl)amino]ethyl}-biphenyl- 4-carboxylic acid (Compound 104)
Figure imgf000072_0001
Figure imgf000072_0002
To a solution of bromide 6.18 (56 mg, 0.10 mmol) and 3,5-pyrimidineboronic acid (22mg, 0.18 mmoL) in DMF (5 mL) is added sodium carbonate solution (2M, 0.2 mL, 0.4 mmol). The mixture is degassed using N2 for 5 min before PdCl2(dppf)CH2θ2 (7 mg, 0.01 mmoL) is added. The mixture is heated at 90 0C overnight. The mixture is concentrated and purified by silica gel chromatography using MeOH-DCM 0-5% (gradient) to give 67 mg of the cross coupling product.
The product from above (46 mg, 0.077mmol) is dissolved in DCM (2 mL) and treated with trifluoroacetic acid (TFA, 0.2 mL). The mixture is stirred at room temperature overnight and concentrated. The residue is washed with acetonitrile to give 29 mg of the title compound 104 as a white solid.
Example 7: Synthesis of 4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]propyl}biphenyl-4- carboxylic acid (Compound 95)
Figure imgf000073_0001
7.21
Figure imgf000073_0002
Figure imgf000073_0003
To a solution of 4-bromophenylacetone (2 g, 9.4 mmol) in methanol (50 mL) is added ammonium acetate (10.8 g, 140 mmol) followed by Na(CN)BH3 (3g, 48 mmol). The mixture is heated to 70 0C (reflux) overnight. After cooling to 0 0C, H2O (100 niL) is added. 50% NaOH solution is then added to the mixture to adjust pH to -10. The mixture is then stirred at room temperature for 2 hours, extracted with DCM (10OmL x 3), dried with Na2SO4 and concentrated to give 2.37 g of the crude reductive amination product as a yellowish oil.
To a solution of the crude product from above (Ig, -4.67 mmol) in DCM cooled to 0 0C is added TEA (3 niL, 21.4 mmol) followed by CbzCl (0.7 niL, 4.67 mmol) drop-wise. The mixture is then stirred at room temperature overnight. The mixture is poured into H2O (50 mL), extracted with DCM, dried with Na2SO4, and concentrated. Silica gel chromatography of the residue using 25% ethyl acetate-hexane gives 710 mg of intermediate 7.21.
To a solution of bromide 7.21 (500 mg, 1.44 mmol) and boronic acid 2 (319 mg, 1.44 mmol) in DMF (10 mL) is added cesium carbonate (936 mg, 2.87 mmol) followed by palladium acetate (16 mg, 0.072 mmol). The mixture is degassed using an Ar stream and stirred at 90 0C overnight. The mixture is cooled to room temperature, filtered through diatomaceous earth, and partitioned between EtOAc, and water. The organic layer is dried over Na2SO4 and concentrated. Silica gel chromatography of the residue provides 360 mg of intermediate 7.22
To a mixture of 7.22 (340 mg, 0.76 mmol) and Pd-C (10%, 80 mg) in EtOAc (5 mL) and MeOH (5 mL) is added cyclohexene (5 mL). The mixture is then heated in a sealed vial under N2 at 70 0C overnight. The mixture is cooled, filtered, and concentrated to give the crude amine 7.23. To a solution of 7.23 (0.7 mmol), 5-chlorosalicyclic acid (150 mg, 0.87 mmol) in DMF (5 niL) is added HOBT (235 mg, 1.74 mmol) then EDC (333mg, 1.74 mmol) followed by DMAP(IO mg). The mixture is stirred at room temperature overnight and concentrated. SGC with 20% E-H gives -500 mg of yellowish oil. The product thus obtained may be further purified by SGC using 0-15% E-H to give 105 mg of 7.24 as a white solid.
To a solution of compound 7.24 (85 mg, 0.18 mmol) in DMF (5 niL) is added NaHCO3 (43mg, 0.52 mmol) then butyl bromide (37 microL, 0.34 mmol). The mixture is heated at 60 0C overnight under N2, concentrated and purified by GSC using 0-5% DCM-MeOH to give 95 mg of 7.25 as thick oil, which turned into a glassy material upon drying under vacuum (99%).
To a solution of 7.25 (70 mg, 0.13 mmol) in DCM (1 niL) is added TFA (0.2 niL). The mixture is stirred at room temperature for 4 hours and concentrated to give 61 mg of the title compound 95.
Example 8: Synthesis of 4'-[2-(2-butoxy-5-chlorobenzoylamino)-l-methyl-ethyl]-biphenyl- 4-carboxylic acid (Compound 71)
Figure imgf000076_0001
8.27
Figure imgf000076_0002
Figure imgf000076_0003
Into a 250 mL round-bottomed flask are placed 4-bromophenyl acetonitrile (2.5 g, 12.7 mmol), (4-?-butylcarbonyl)boronic acid (2.82 g, 12.7 mmol), and palladium (II) acetate (0.25 g, 1.11 mmol). A 2 M solution Of Cs2CO3 (10.5 mL) is added to the flask followed by DMF (50 mL) and the mixture is heated at 90 0C for 18 h. The reaction mixture is cooled, diluted with EtOAc (100 mL), and washed with water (5 x 100 mL), 2 M HCl (50 mL), and brine (100 mL). The organic layer is dried over Na2SO4, filtered, concentrated to dryness, and the resulting residue may be purified by flash silica gel chromatography (eluent: 15% EtOAc in hexanes) to give 8.27 (1.25 g, 37%) as a yellow solid.
Sodium hydride (60% in mineral oil, 34 mg, 0.85 mmol) is placed into a 100 mL round- bottomed flask and washed with hexanes. A solution of biphenyl acetonitrile 8.27 (0.25 g, 0.85 mmol) in DMF (15 mL) is added to the flask, and its contents is stirred for 30 min at room temperature. Iodomethane (0.12 g, 0.85 mmol) is then added and the reaction mixture stirred overnight. The reaction mixture is diluted with EtOAc (25 mL) and washed with brine (7 x 25 mL). The organic layer is separated, dried over Na2SO4, filtered and evaporated to dryness to give a crude solid that is purified by flash silica gel chromatography (eluent: 15% EtOAc in hexanes). Fractions are combined and evaporated to give 8.28 (0.2 g, 77%) as a light yellow solid.
Nitrile 8.28 (0.3 g, 1.0 mmol) is dissolved in MeOH and added to 50% Raney nickel and subjected to hydrogenation at 50 psi overnight in a Parr shaker. The reaction mixture is filtered through a short pad of diatomaceous earth and concentrated to dryness to give 0.20 g of crude 8.29 (67%) of as a dark yellow solid that is usable for the next reaction without purification.
Amine 8.29 (0.20 g, 0.64 mmol), 2-butoxy-5-chlorobenzoic acid (0.15 g, 0.65 mmol), EDC'HCl (0.15 g, 0.77 mmol), HOBt (0.10 g, 0.77 mmol) and TEA (0.08 g, 0.77 mmol) are dissolved in CHCI3 (10 mL) and stirred overnight. The reaction mixture is washed with water (10 mL), 1 M HCl (2 x 10 mL), and brine (10 mL), dried over Na2SO4, filtered and evaporated to dryness to give the crude product. Purification by flash silica gel chromatography (eluent: 20% EtOAc in hexanes) gives the ?-butyl ester of 71 (0.10 g, 30%) as an off-white solid. The f-butyl ester (0.10 g, 0.24 mmol) is dissolved in 3:1 CH2C12/TFA (40 mL), stirred for 12 h, and evaporated to dryness to give the title compound 71 (80 mg, 88%) as an off-white solid.
Example 9: Synthesis of 4'-[2-(2-butoxy-5-chloro-benzoylamino)-l,l-dimethyl-ethyl]- biphenyl-4-carboxylic acid (Compound 94)
Ni
Figure imgf000078_0001
Figure imgf000078_0002
Sodium hydride (60% in mineral oil, 0.19 g, 4.7 mmol) is weighed into a 100 mL round- bottomed flask and washed with hexanes. A solution of 9.27 (0.55 g, 1.9 mmol) in DMF (20 mL) is added to the flask, and its contents is stirred for 30 min at room temperature. Iodomethane (0.80 g, 5.6 mmol) is then added and the reaction mixture stirred overnight. The reaction mixture is diluted with EtOAc (50 mL) and washed with brine (7 x 50 mL). The organic layer is separated, dried over Na2SO4, filtered and evaporated to dryness to give a crude solid which is purified by flash silica gel chromatography (eluent: 15% EtOAc in hexanes). Fractions are combined and evaporated to give 9.31 (0.35 g, 58%) as a yellow solid. Nitrile 9.31 (0.35 g, 1.1 mmol) is dissolved in MeOH and added to 50% Raney nickel (0.2 g) and subjected to hydrogenation at 45 psi overnight in a Parr shaker. The reaction mixture is filtered through a short pad of diatomaceous earth and concentrated to dryness to give 0.21 g (49%) of crude 9.32 as a dark yellow solid that is usable for the next reaction without purification.
Amine 9.32 (0.17g, 0.53 mmol), 2-butoxy-5-chlorobenzoic acid (0.12 g, 0.53 mmol), EDC'HCl (0.12 g, 0.64 mmol), HOBt (86 mg, 0.64 mmol) and TEA (64 mg, 0.64 mmol) are dissolved in CHCI3 (10 mL) and stirred overnight. The reaction mixture is washed with water (10 mL), 1 M HCl (2 x 10 mL), and brine (10 mL), dried over Na2SO4, filtered and evaporated to dryness to give the crude product. Purification by flash silica gel chromatography (eluent: 20% EtOAc in hexanes) gives the ?-butyl ester of 28 (0.12 g, 50%) as an off-white solid. The ?-butyl ester (0.12 g, 0.22 mmol) is dissolved in 3:1 CH2C12/TFA (40 mL), stirred for 12 h and evaporated to dryness to give the title compound 94 (51 mg, 50%) as an off-white solid.
Example 10: Synthesis of 4'-[2-(2-Butoxy-5-chloro-benzoylamino)-2-methyl- propyl]biphenyl-4-carboxylic acid (Compound 137)
Figure imgf000080_0001
9.34
,CO2tBu H)2OB('
Figure imgf000080_0002
Figure imgf000080_0003
A solution of 4-nitrobenzyl chloride (2.0 g, 11.7 mmol) in benzene (15 mL) is stirred under nitrogen with a solution of 2-nitropropane (1.5 g, 16.8 mmol) and 1 M tetrabutylammonium hydroxide in water (25 mL) for 3 h. More benzene (100 mL) is added to the reaction mixture followed by the separation of phases, drying of the organic layer over Na2SO4, filtration to remove the drying agent and the evaporation of solvents. The resulting crude solid is purified by flash silica gel chromatography (eluent: 15% EtOAc in hexanes) to give 10.34 (1.6 g, 62%).
To a solution of 10.34 (1.5 g, 6.7 mmol) in MeOH (50 niL) is added 10 wt. % Pd-C (0.7 g, 0.67 mmol). The reaction mixture is then subjected to hydrogenation at 1 atm and is stopped when 10.34 is totally consumed. The diamine over-reduction product is separated from the desired monoamine product by a flash silica gel chromatography (eluent: 50% CH2Cl2 in hexanes) to give 10.35 (0.65 g, 50%).
?-Butyl nitrite (0.52 g, 5.0 mmol) and CuBr2 (0.94 g, 4.0 mmol) are weighed into a 100 mL 3- necked round-bottomed flask fitted with a condenser, addition funnel and a gas outlet tube. Anhydrous acetonitrile (10 mL) is added and the mixture is heated to 65 0C. A solution of 10.35 (0.65 g, 3.4 mmol) in acetonitrile (7 mL) is added to the heated reaction mixture drop wise with vigorous stirring. Heating and stirring is continued for 16 h after which the resulting black reaction mixture is cooled and poured into 20% aqueous HCl (100 mL). The aqueous acetonitrile mixture is extracted with diethyl ether (2 x 100 mL) and the combined ether extracts are dried over MgSO4, filtered and evaporated to dryness to give a yellow viscous oil (0.84 g, 99%). 1H NMR analysis indicates the product is a 3:1 mixture of the desired 4-bromo compound 10.36 and the 3,4-dibromo by-product. This mixture is taken to the next step without further purification.
Into a 50 mL round-bottomed flask are weighed crude 10.36 (0.63 g, 2.4 mmol), (4-t- butylcarbonyl)boronic acid (0.73 g, 3.3 mmol), and palladium (II) acetate (73 mg, 0.33 mmol). A 2 M solution of Cs2CO3 (3.0 mL) is added to the flask followed by DMF (20 mL), and the mixture is heated at 90 0C for 18 h. The reaction mixture is cooled, diluted with EtOAc (10 niL) and washed with water (5 x 10 niL), 2 M HCl (10 niL) and brine (10 niL). The organic layer is dried over Na2SO4, filtered, concentrated to dryness and the resulting residue is purified by trituration with MeOH to give 10.37 (0.12 g, 22%) as an off-white solid.
A solution of the biphenyl nitro compound 10.37 (0.12 g, 0.34 mmol) in MeOH (25 niL) is added to 10 wt. % Pd-C and subjected to hydrogenation at 40 psi overnight. The reaction mixture is filtered through a short pad of diatomaceous earth and the filtrate evaporated to dryness to give crude 10.38 (71 mg, 67%) as a viscous oil which is taken to the next step without purification.
Crude amine 10.38 (71 mg, 0.22 mmol), 2-butoxy-5-chlorobenzoic acid (49 mg, 0.22 mmol), EDC'HCl (50.2 mg, 0.26 mmol), HOBt (35.3 mg, 0.26 mmol) and TEA (26.4 mg, 0.26 mmol) are dissolved in CHCl3 (10 niL) and stirred overnight. The reaction mixture is washed with water (10 niL), 1 M HCl (2 x 10 niL) and brine (10 niL), dried over Na2SO4, filtered and evaporated to dryness to give the crude product. Purification by preparative TLC (eluent: 100% CH2Cl2) gives the f-butyl ester of compound 137 (18 mg, 12%) as an off-white solid. The f-butyl ester (18 mg, 0.033 mmol) is dissolved in 50% TFA in CH2Cl2 (5 niL), stirred for 12 h, and evaporated to dryness to give crude compound 137. After trituration with EtOAc and hexanes, the title compound 137 (3 mg, 19%) is isolated as an off-white solid.
Example 11: Synthesis of 4'-{(lS,2S)-2-[(2-butoxy-5-chlorobenzoyl)amino]cyclopropyl}- biphenyl-4-carboxylic acid (Compound 114)
Figure imgf000083_0001
Rh2(OAc)4
BnEt3NCl dioxane
Figure imgf000083_0002
4-Bromobenzaldehyde (6.0 g, 32.4 mmol) is added in portions to a suspension of tosylhydrazide (6.93 g, 37.2 mmol) in MeOH (30 mL). The mixture is stirred for 1 h at room temperature then cooled to 0 0C and filtered. The white solid is washed with cold MeOH and dried to afford 11.40 (9.2 g, 80%).
A solution of the hydrazone 11.40 (2.5 g, 7.1 mmol) in anhydrous THF (60 mL) is cooled to - 78 0C and 1 M sodium hexamethyldisilazane (7.1 mL, 7.1 mmol) is added. The mixture is maintained at -78 0C for 15 min. then allowed to warm to room temperature. The solvent is removed under vacuum leaving a light yellow residue which is suspended in 1 ,4-dioxane (100 mL). To the suspension is added N-vinylphthalimide (7.3 g, 42.1 mmol), benzyltriethylammonium chloride (0.20 g, 0.88 mmol), and rhodium diacetate dimer (38 mg, 0.086 mmol). The resulting mixture is heated to 50 0C for 48 h. EtOAc (200 mL) is added and the mixture is washed with water (4 x 100 mL). The organic layer is separated, dried over Na2SO4, filtered and evaporated to dryness to give the crude solid that is purified by a flash silica gel chromatography (eluent: 10% EtOAc in hexanes) to give 11.41 (1.0 g, 40%) as a white solid.
A solution of hydrazine monohydrate (52.6 mg, 1.1 mmol) in ethanol (5 mL) is added to a suspension of cw-4-bromophenyl-cyclopropyl phthalimide 11.41 (0.30 g, 0.88 mmol) in ethanol (10 mL). After stirring for 15 h at 40 0C, the reaction mixture is filtered and the filtrate evaporated leaving a viscous oil. To the oil is added 2-butoxy-5-chlorobenzoic acid (0.20 g, 0.88 mmol), EDC'HCl (0.20 g, 1.1 mmol), HOBt (0.14 g, 1.1 mmol) and TEA (0.11 g, 1.1 mmol). The mixture is dissolved in CHCl3 (10 mL) and stirred overnight. The reaction mixture is washed with water (10 mL), 2 M HCl (2 x 10 mL), brine (10 mL), dried over Na2SO4, filtered and evaporated to dryness to give the crude product that is purified by flash silica gel chromatography (eluent: 10% EtOAc in hexanes). Evaporation of the solvents gives 11.42 (68 mg, 18%) as a white solid;
To a solution of compound 11.42 and boronic acid 11.2 (12 mg, 0.028 mmol) in DMF (0.5 mL) is added sodium carbonate solution (2M, 0.1 mL). The mixture is degassed using N2 for 5 min before PdC12(dppf).DCM (2 mg, 0.002 mmol) is added. The mixture is then heated at 70 0C overnight and concentrated. The residue is purified by prep-TLC (33% E-H) to give 12 mg of 11.43 as white solid. To a solution of 11.43 (10 mg, 0.019 mmol) in THF (0.5 niL) is added 10% KOH solution (0.1 mL). The mixture is heated at 50 0C overnight and concentrated. The residue is suspended in 2 mL of water and acidified with solid citric acid to pH ~ 5. The precipitate is collected by centrifugation, washed with water (2x1 mL), dried under vacuum to give 6 mg title compound 114 as white solid.
Example 12. Synthesis of 4'-{(lR,2S)-2-[(2-butoxy-5-chlorobenzoyl)amino]- cyclopropyl}biphenyl-4-carboxylic acid (Compound 108)
Figure imgf000086_0001
neat 12.45 38% 95% 12.46
Ref Org Lett 2003, 5, 2327
Figure imgf000086_0002
Figure imgf000086_0003
To a mixture of 4-bromostyrene (5.75 g, 31.4 mmol) and rhodium (II) trimethylacetate dimer (47 mg, 0.077 mmol) is added methyl nitroacetate (934 mg, 7.85 mmol) followed by iodobenzene diacetate (3.43 g, 10.65 mmol). After stirring at room temperature for 48 h, the crude mixture is applied directly to a silica gel column and flash chromatographed (hexanes to 93:7 hexanes/ethyl acetate) to afford 12.45 (2.24 g, 95%) as a colorless oil. To a solution of ester 12.45 (900 mg, 2.87 mmol) in methanol (30 niL) is added 2 N NaOH (6 mL). After stirring overnight at room temperature, TLC analysis indicates complete consumption of the starting material. The solvent is removed at reduced pressure, and the residue is diluted with water and acidified to pH 2 with concentrated hydrochloric acid. The mixture is extracted with ethyl acetate (3 x 50 mL) and the combined extracts are washed with brine, dried over sodium sulfate, filtered, and concentrated at reduced pressure to afford
12.46 (775 mg, 95%) as a white foam.
A solution of acid 12.46 (2.40 g, 8.39 mmol) and sodium bicarbonate (705 mg, 8.39 mmol) in DMSO (40 mL) and water (4 mL) is heated to 60 0C for 30 min. The mixture is cooled, acidified with 1 N HCl, and extracted with ethyl acetate (3 x 50 mL). The extracts are combined, washed with brine (5 x 100 mL), dried over sodium sulfate, filtered, and concentrated at reduced pressure. The residue is flash chromatographed (silica gel, hexanes to 95:5 hexanes/ethyl acetate) to afford trans-l2Λ7 (770 mg, 38%) as an oil.
A 2 M cesium carbonate solution (1.75 mL) is added to a solution of nitrocyclopropane trans-
12.47 (345 mg, 1.43 mmol) and 4-(te?t-butoxycarbonyl)phenyl boronic acid (290 mg, 1.31 mmol) in DMF (10 mL). The mixture is degassed with three evacuation and argon backfill cycles. Palladium acetate (30 mg, 0.13 mmol) is added, and the solution is degassed again and heated to 80 0C. After 5 h, the mixture is cooled. Ethyl acetate and 1 N HCl are added, and the mixture is filtered through diatomaceous earth. The filtrate layers are separated, and the aqueous layer is extracted with ethyl acetate (2 x 30 mL). The organic layers are combined, washed with brine, dried over sodium sulfate, filtered, and concentrated at reduced pressure. The residue is flash chromatographed (Biotage 12 M silica cartridge, hexanes to 92:8 hexanes/ethyl acetate) to afford 12.48 (270 mg, 61%) as a white solid. Zinc dust (700 mg, 10.71 mmol) is added in portions over 10 min to a suspension of nitrocyclopropane 12.48 (180 mg, 0.53 mmol) in 2-propanol (11 niL) and 1 N HCl (5.4 niL). After stirring at room temperature for 45 min, saturated sodium bicarbonate is added, and the mixture is filtered through diatomaceous earth. The filter cake is rinsed thoroughly with ethyl acetate, and the filtrate layers are separated. The aqueous layer is extracted with ethyl acetate (2 x 30 mL), and the organic layers are combined, washed with brine, dried over sodium sulfate, filtered, and concentrated at reduced pressure. The residue is flash chromatographed (silica gel, dichloromethane to 99:1 dichloromethane/10% cone, ammonium hydroxide in methanol) to afford 12.49 (41 mg, 25%) as a white solid.
EDC (83 mg, 0.43 mmol), HOBt (60 mg, 0.44 mmol), Hunig's base (86 mg, 0.66 mmol) and DMAP (3 mg, 0.024 mmol) are added to a stirred solution of amine 49 (68 mg, 0.22 mmol) and 2-butoxy-5-chlorobenoic acid (86 mg, 0.38 mmol) in DMF (2 mL). After stirring overnight at room temperature, ethyl acetate and water are added. The layers are separated, and the aqueous layer is extracted with ethyl acetate (2 x 20 mL). The organic extracts are combined, washed with brine (5 x 20 mL), dried over sodium sulfate, filtered, and concentrated at reduced pressure. The residue is flash chromatographed (hexanes to 93:7 hexanes/ethyl acetate) to afford amide 12.50 (101 mg, 89%) as a foam.
A 2 N NaOH solution (3 mL) is added to a solution of amide 12.50 (80 mg, 0.15 mmol) in methanol (5 mL) and THF (5 mL). After stirring overnight at 50 0C, the solution is cooled and the volatiles are removed at reduced pressure. The residue is diluted with water, and acidified to pH 3 with 1 N HCl. The resulting white solid is isolated by filtration and dried at reduced pressure to afford the title compound 108 (67 mg, 94%); mp 218-222 0C. Example 13. Synthesis of 2-{4'-[2-(2-butoxy-5-chloro-benzoylamino)-ethyl]-3'-ethyl- biphenyl-4-yloxy}-2-methyl-propionic acid (Compound 158)
Figure imgf000089_0001
To a solution of 4-bromo-2-ethylphenol (535 mg, 2.66 mmol) and boronic ester 13.10 (750 mg, 2.24 mmol) in DMF (7 niL) is added PdCl2(dppf)CH2Cl2 (46 mg, 0.021 mmol) then Na2COs solution (2M, 3mL). The mixture is degassed using Ar stream for 10 min, sealed under Ar and then heated at 800C overnight. The mixture is concentrated. The residue is suspended in 10% citric acid and extracted with EtOAc. The organic layer is dried with Na2SO4, concentrated, and purified by SGC to give 545 mg of cross coupling product.
The product above (470mg, 1.43 mmol) is dissolved in dioxane. To this solution is added Hunig's base (41 microL, 0.23 mmol) and PhNTf2 (614 mg, 1.74 mmol). The mixture is stirred at 60 0C for 24 hours and concentrate. The residue is purified by SGC to give 560 mg of product 13.52 as a white solid. A mixture of compound 52 (257mg, 0.56 mmol), dry LiCl (54 mg, 1.27 mmol) and bis(triphenylphosphine)palladium(II) chloride (20mg, 0.028 mmol) in 1 mL of dry DMF is degassed with Ar for 10 min before tributyltin (265mg, 0.84 mmol) is added. The mixture is degassed 5 min more and sealed in a vial under Ar. The mixture is heated at 90 0C for 6 hours. After removal of DMF, the residue is purified by SGC using 0-10 % E-H to give 72 mg of colorless oil (compound 13.53, yield 68%).
To a solution of compound 13.53 (lOOmg, 0.3 mmol) in THF (0.2 mL) is added catecholborane (IM, 350 microL, 0.35 mmol) then tris(triphenylphosphine)rhodium(I) chloride (3mg, 0.030 mmol) under Ar. The mixture is stirred at room temperature for 14 hours. To this mixture, MeOH (1 mL) is carefully added at 0 0C. Hydroxylamine sulfonic acid (260mg, 2.3 mmol) is added next followed by NaOH (2M, 0.3 mL). The mixture is stirred at 25 0C for 24 hours. LC-MS shows a major product peak (ES+ 339). The mixture is cooled to 0 0C, adjusted to pH ~4, and filtered. The filtrate is purified directly by reverse phase HPLC to give 35 mg of compound 13.54 as yellowish oil.
To a solution of 5-chloro-2-butoxyoxy-benzoic acid (20 mg, 0.087 mmol) in DMF (0.2 mL) is added HOBT (12 mg, 0.087 mmol) then EDC (17 mg, 0.087 mmol). The mixture is stirred at room temperature for 2 hours. To this mixture is then added compound 13.54 (19mg, 0.053 mmol) followed by Hunig's base (46microL, 0.262 mmol) and DMAP (2 mg, 0.016 mmol). The mixture is stirred at room temperature overnight and then heated at 50 0C for 2 hours. The mixture is concentrated and the residue purified by Prep-TLC using 30% E-H to give 26 mg of coupling product (ester), yield 53%. To a solution of ester 13.54 (2 mg, 0.004mmol) in 0.1 mL of acetic acid is added 0.1 mL of 48% HBr-H2O solution. The mixture is stirred at room temperature for 18 h and concentrated. The residue is purified by Prep-TLC (silica gel) to give ~1 mg of the title compound 158.
Example 14: Synthesis of 2-[(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}-3'- fluorobiphenyl-4-yl)oxy]-2-methylpropanoic acid (Compound 54)
Figure imgf000091_0001
Reagents and Conditions: a) MeNO2, NH4Ac, HOAc, 110 0C, 3 h; b) LiBH4, TMSCl, 23 0C, 24 h; c) EDC, HOBt, /-Pr2NEt, DMF, 15 h; d) PdCl2(dppf)CH2Cl2, 2 M aq. Na2CO3, DME, 85 0C, 14 h; e) NaOH, THF-MeOH, 70 0C, 2 h. (795-025).
A mixture of 4-bromo-2-fluorobenzaldehyde (4 g, 19.7 mmol), nitromethane (10 mL, 1643.8 mmol), and ammonium acetate (1.4 g, 23.0 mmol) in 6 mL HOAc is heated at 110 0C for 3 h. The reaction mixture is cooled down, diluted with water and extracted with EtOAc. The extract is washed with 2 M NaOH, sat. Na2CO3, brine, dried over Na2SO4, and concentrated in vacuo. The crude product is purified by chromatography to give the desired olefin 14.56 (3.7 g, 76%).
A solution of Me3SiCl (1 M, 3 ml, 3 mmol) in THF is added to a solution of LiBH4 (2 M, 0.7 ml, 1.4 mmol) in THF (5 mL) under Ar2. After 5 min, 56 (180 mg, 0.73 mmol) is added in 2 mL THF and the mixture is stirred at 23 0C for 24 h. The reaction is carefully quenched by adding 10 mL of methanol at 0 0C. After the solvents are removed in vacuo, the residue is diluted with CH2Cl2, washed with 0.5 M aq. NaOH, brine, dried over Na2SO4, and concentrated to give 150 mg of crude product 14.57 as a brown oil that is usable in the next step without further purification.
A mixture of 5-chloro-2-methoxybenzoic acid (100 mg, 0.54 mmol), the above amine (150 mg, 0.69 mmol), EDC (150 mg, 0.78 mmol), HOBt (110 mg, 0.81 mmol), Hunig's base (0.2 mL, 1.15 mmol) in 1 mL of DMF is stirred at 23 0C for 14 h. The reaction mixture is diluted with EtOAc, washed with water, brine, dried over Na2SO4, and concentrated in vacuo. The crude product is purified by chromatography to give the desired amide 14.58 (120 mg, 57% in 2 steps).
To a solution of the boronic acid pinacol ester (120 mg, 0.36 mmol) in DME (1 mL) is added 14.58 (120 mg, 0.31 mmol). The mixture is purged with Ar2. PdCl2(dppf)CH2Cl2 (25 mg, 0.031 mmol) is then added followed by an aq. solution of Na2CO3 (2 M, 0.31 mL, 0.62 mmol) under Ar2. The reaction tube is then sealed and the reaction mixture is heated at 85 0C for 14 h. The reaction mixture is diluted with EtOAc, washed with water, brine, dried over Na2SO4, and concentrated in vacuo. The crude product is purified by chromatography eluting with 20-50% EtOAc in hexane to give the desired cross coupling product (85 mg, 53%). To a solution of the above product (80 mg, 0.16 mmol) in 3 mL of 1 : 1 THF-MeOH is added aq. NaOH (2 M, 0.15 mL, 0.3 mmol). The mixture is heated at 70 0C for 2 h. After cooling down, the reaction mixture is diluted with water, acidified with 3 N HCl to pH 2-3, and extracted with EtOAc, washed with brine, dried over Na2SO4, and concentrated in vacuo to give the title compound 54 (65 mg, 86%).
Example 15: Synthesis of 2-(4-{5-[2-(2-butoxy-5-chloro-benzoylamino)-ethyl]-pyridin-2- yl}-phenoxy)-2-methyl-propionic acid (Compound 157)
Figure imgf000093_0001
A mixture of 6-bromo-pyridine-3-carbaldehyde (2 g, 10.8 mmol), nitromethane (6 mL, 98.3 mmol) and ammonium acetate (0.8 g, 13.1 mmol) in 5 mL HOAc is heated at 1000C for 3 h. The reaction mixture is cooled, the precipitates are collected by filtration, washed with ether, and dried to give the condensation product (1.5 g, 61%). To a suspension of the above product (500 mg, 2.18 mmol) in 5 mL MeOH-CH2Cl2 (2:3) is added NaBH4 (200 mg, 5.26 mmol) in portions at 0 0C and the resulting solution is stirred at 23 0C for 1 h. The reaction is then quenched by adding 10% NH4Cl, diluted with EtOAc, washed with sat. aq. NaHCU3, brine, dried over Na2SO4, and concentrated in vacuo to give the desired product 15.60 (450 mg, 89%) which is used in next step without any purification.
To a solution of the boronic acid pinacol ester 15.10 (200 mg, 0.60 mmol) in DMF (1 mL) is added the bromide 15.60 (130 mg, 0.56 mmol). The mixture is purged with Ar2. PdCl2(dppf) CH2Cl2 (50 mg, 0.062 mmol) is then added followed by an aq. solution of Na2Cθ3 (2 M, 0.6 mL, 1.2 mmol) under Ar2. The reaction tube is then sealed and the reaction mixture is heated in a microwave reactor at 120 0C for 30 min. The reaction mixture is diluted with EtOAc, washed with water, brine, dried over Na2SO4, and concentrated in vacuo. The crude product is purified by chromatography eluting with 40-80% EtOAc in hexane to give the desired product 15.61 (25 mg, 12%).
To a solution of the above nitro compound 15.61 (25 mg, 0.07 mmol) in 1 mL of EtOH is added 10% Pd-C (10 mg), and the mixture is stirred under one atmosphere H2 gas for 2 days. The reaction mixture is filtered through a pad of diatomaceous earth, and the solid residue is rinsed with CH2Cl2. The filtrate is concentrated in vacuo to give 25 mg of crude product 15.62, which is used in next step.
A mixture of 5-chloro-2-«-butoxybenzoic acid (16 mg, 0.07 mmol), amine 62 (25 mg, 0.07 mmol), EDC (30 mg, 0.16 mmol), HOBt (20 mg, 0.15 mmol), Hunig's base (0.03 mL, 0.17 mmol) in 1 mL of DMF is stirred at 23 0C for 14 h. The reaction mixture is diluted with EtOAc, washed with water, brine, dried over Na2SO4, and concentrated in vacuo. The crude product is purified by chromatography to give the desired amide (15 mg, 40% in 2 steps).
To a solution of the above product (15 mg, 0.028 mmol) in 2 mL of 1:1 THF-MeOH is added aq. NaOH (1 M, 0.1 mL, 0.1 mmol). The mixture is heated at 70 0C for 3 h. After cooling down, the reaction mixture is diluted with water, acidified with 3 M HCl to pH ~4, and extracted with EtOAc, washed with brine, dried over Na2SO4, and concentrated in vacuo. The crude product is purified by preparative HPLC to give the title compound 157 (11 mg, 63%) as the TFA salt. .
Example 16: Synthesis of 4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-3- chlorobiphenyl-4-carboxylic acid (Compound 11)
Figure imgf000095_0001
To a 1.0 L, round-bottomed flask are added methyl 5-chlorosalicylate (21.3 g, 0.114 mol), K2CO3 (18.0 g, 0.131 mol) and iodobutane (24.0 g, 0.131 mol). Acetone (400 mL) is added to the flask and its contents are heated to reflux for 16 h. The reaction mixture is cooled to room temperature and filtered. The filtrate is evaporated to dryness leaving a gummy solid that is redissolved in EtOAc, washed with saturated NH4Cl solution, dried over Na2SO4, filtered and evaporated to dryness to give 16.64 (25.3 g, 91%) as a white sticky solid.
A solution of NaOH (8.3 g, 0.21 mol) in water (100 mL) is added to a solution of methyl 2- butoxy-5-chlorobenzoate 16.64 (23.5 g, 0.10 mol) in MeOH (70 mL) and the resulting mixture is stirred at room temperature for 4 h. After evaporation of most of the solvent, the resulting suspension is diluted with water (150 mL), acidified to pH 2 with cone. HCl and extracted with EtOAc. The organic layer is separated, dried over Na2SO4, filtered and evaporated to dryness to give 16.65 (18.2 g, 79%) as an off-white solid.
2-Butoxy-5-chlorobenzoic acid 65 (13.0 g, 57.0 mmol), 4-bromophenethylamine (11.4 g, 57.0 mmol), EDC'HCl (13.1 g, 68.4 mmol), HOBt (9.2 g, 68.4 mmol) and TEA (6.9 g, 68.4 mmol) are dissolved in CHCI3 and stirred overnight. The reaction mixture is washed with water (250 mL), 1 M HCl (3 x 250 mL), and brine (200 mL), dried over Na2SO4, filtered and evaporated to dryness to give the crude product. Purification by a flash silica gel column chromatography (eluent: 15% EtOAc in hexanes) gives 16.66 (17.0 g, 73%) as a white solid.
To a solution of the bromide 16.66 (100 mg, 0.243 mmol) and (4-methoxycarbonyl 3- chlorophenyl) boronic acid (57.2 mg, 0.267 mmol) in 2 mL of DMF is added an aq. Na2Cθ3 solution (2 M, 243 microL, 0.486 mmol). The mixture is purged with Ar2 for 10 min. PdCl2(dppf)CH2Cl2 (9.7 mg, 0.012 mmol) is then added. The reaction is stirred under Ar2 at 85°C for 18 h. The resulting coupled product ester is hydrolyzed in situ with the addition of 243 microL of 2M Na2Cθ3 solution and heating at 120 0C. After cooling down, the reaction mixture is extracted with EtOAc, and washed with water, brine. The organic layer is separated and dried over MgSO4 and concentrated in vacuo. Purification of the crude product by reverse phase HPLC gives the title compound 11 (25 mg, 21%).
Example 17: Synthesis of 2-[(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-3- fluorobiphenyl-4-yl)oxy]-2-methylpropanoic acid (Compound 4)
Figure imgf000097_0001
To a solution of the bromide 17.66 (2.0 g, 4.869 mmol) and Bis(pinacolato)diboron (1.35 g, 5.34 mmol) in 16 mL of anhydrous DMSO is added KOAc (954 mg, 9.72 mmol). The mixture is purged with Ar2 for 10 min. PdCl2(dppf)CH2Cl2 (195 mg, 0.24 mmol) is then added. The reaction is stirred under Ar2 at 120 0C for 18 h. After cooling down, the reaction mixture is extracted with EtOAc, and washed with water and brine. The organic layer is separated and dried over MgSO4 and concentrated in vacuo. Purification of the crude product chromatography gives the desired product 17.68 (1.68 g, 75.4%). To a solution of 4-bromo-2-fluorophenol (921 mg, 4.82 mmol) and l,l,l-trichloro-2-methyl- 2-propanol hemihydrate (1.711 g, 9.64 mmol) in acetone (23 ml) is added NaOH (1.542 g, 38.56 mmol) and the mixture is stirred at room temperature for 16h. After solvent is removed under reduced pressure, the residue is dissolved in water and washed with ether. The aqueous layer is acidified with concentrated HCl and extracted with ether. The extracts are washed twice with brine, dried over anhydrous MgSO4, and the solvent is removed under reduced pressure to give crude acid product as a waxy solid.
The residue is dissolved in EtOH (23 niL) and SOCl2 (399 microL, 4.82 mmol) and is carefully added drop- wise to the stirred solution at room temperature. The mixture is heated under reflux for 6h. After the reaction mixture is concentrated under reduced pressure, the residue is dissolved in ether and washed with water, saturated aqueous NaHCU3, and brine successively. The organic layer is dried over anhydrous MgSO4 and concentrated under reduced pressure. The crude product is purified by a chromatography (eluted with hexane to 30%EtOAc/hexane) to give the desired product 17.69 as a colorless oil (1.05 g, 71.4% in two steps).
To a solution of the boronic acid pinacol ester 17.68 (100 mg, 0.218 mmol) in DME (2 mL) is added the bromide 17.69 (80.5 mg, 0.264 mmol). The mixture is purged with Ar2. PdCl2(dppf)CH2Cl2 (9.75 mg, 0.012 mmol) is then added followed by an aq. solution of Na2Cθ3 (2 M, 272 microL, 0.545 mmol) under Ar2. The reaction tube is then sealed and the reaction mixture is heated at 120 0C for 7 h. After cooling down, the reaction mixture is diluted with EtOAc, washed with water, brine, dried over MgSO4, and concentrated in vacuo. The crude ester product is passed through a short silica column to give 17.70 as yellowish oil which is used for the saponification step without further purification. To a solution of the above ester 17.70 (122 mg, 0.219 mmol) in 1 mL of THF-EtOH (1: 1 v/v) is added aq. NaOH (2 M, 219 microL, 0.438 mmol). The mixture is stirred at room temperature overnight. The reaction mixture is acidified with 3 N HCl to pH 2-3 and diluted with 1.5 mL of water-MeCN-DMSO (1:1 :1). The crude product is obtained by filtration and is purified by reverse phase HPLC to provide the title compound 4 as a colorless oil (69 mg, 60% in two steps).
Example 18. Synthesis of 2-{3',5'-Dichloro-4'-[2-(5-chloro-2-methoxy-benzoylamino)- ethyl]-biphenyl-4-yloxy}-2-methyl-propionic acid (Compound 159)
Figure imgf000099_0001
PhNTf2
Figure imgf000099_0002
Figure imgf000099_0003
A solution of 4-bromo-2,6-dichlorophenol (270mg, 1.12 mmol), boronic ester 18.10 (373 mg, 1.12 mmol), PdCl2(dppf)CH2Cl2 (90mg, 0.112 mmol) in DMF (10 mL) is degassed with an Ar stream for 10 min before Na2COs (2M solution) is added. The mixture is sealed under Ar and heated at 70 0C for 16 h and concentrated. The residue is suspended in 10% citric acid and then extracted with EtOAc. The organic layer is dried with Na2SO4, concentrated, and purified by SGC (0-30% E-H gradient) to give 320 mg product 18.72.
To a solution of 18.72 (300mg, 0.812 mmol) in dioxane (3 mL) is added diisopropylethylamine (218 micrL, 1.22 mmol) followed by N-phenyl trifluoromethanesulfonimide (348 mg, 0.975 mmoL). The mixture is heated at 50 0C for 16 h. The mixture is concentrated and purified by SGC Biotag, 5% EtOAc - hexane to yield 80 mg desired product 18.73 as white needle-like crystals.
To a flask containing dry LiCl (20mg, 0.472 mmol) and palladium acetate (12 mg, 0.055 mmol) is added a solution of the triflate 18.73 (138mg, 0.275 mmol) in DMF (1.5 mL). The mixture is degassed using an Ar stream for 10 min before vinyltrimethylsilane (200 microL, 1.3 mmol) and) triethyl amine (200 microL, 1.4 mmol) are added. The mixture is then sealed and heated at 55 0C for 20 hr. DMF is removed. The residue is taken up in dichloromethane and purified by SGC using 10% E-H to give 96 mg desired product 18.74.
To a solution of the olefin 18.74 (72mg, 0.19 mmol) in THF (0.2 mL) is added 9-BBN (0.5 M, 0.57 mL, 0.285 mmol) under Ar. The mixture is heated at 50 0C overnight and then cooled to room temperature and concentrated. The residue is re-dissolved in DME (0.4 mL) and treated with hydroxyaminesulfonic acid (96 mg, 0.85 mmol). The mixture is then heated under Ar at 100 0C for 5 hours. After cooling to room temperature, MeOH (0.5 mL) is added followed by 2M Na2CO3 (0.5 mL). The mixture is next stirred at room temperature overnight and then acidified to pH 2 using 2N HCl. The mixture is then extracted with EtOAc, dried (Na2SO4) and concentrated. Purification of the residue by reverse phase HPLC gives 16 mg of amine 18.75.
To a solution of 5-chloro-2-methoxy-benzoic acid (7 mg, 0.038 mmol) in DMF(0.2 mL) is added HOBT (10 mg, 0.074 mmol) and EDC (7 mg, 0.037 mmol). The mixture is stirred at room temperature for 2 hours before a solution of amine 18.75 (5 mg, 0.014 mmol) in DMF (50 microL) and 4-(N,N-dimethylamino)pyridine (catalytic amount) are added. The mixture is stirred at room temperature for 24 h and concentrated. The residue is purified by reverse phase HPLC using 30-100% CH3CN-H2O w/0.1% TFA to give 2 mg of the title compound 159.
Procedures for Identification of CCRlO Antagonists
CCRlO FLIPR Assay
Preferred compounds have an IC50 of 500 nM or lower in this assay.
Cell Media
To a 1 liter bottle of Hams F12 (Mediatech #10-080-CM ) add 100 mL Fetal Bovine Serum (Mediatech #35-015-CV ), 10 mL geneticin (Invitrogen #10131-027), and 2 mL Zeocin (Invitrogen #R250-05).
Cell plating for assay
CHO-Kl hCCRlO cells (Euroscreen cat # ES-143-A) are diluted in media to a final concentration of 2.8 X 105 cells/mL and 25 microL of this suspension are added to each well of a BD384 well TC treated assay plate (VWR #62406-490). This will yield approximately 7,000 cells/well. The plate is incubated at 37°C/5% CO2 overnight.
EC50 determination of CCRlO peptide
The EC50 and EC70 should be calculated each time the assay is performed. CTACK/CCL27 (R&D Systems # 376-CT; 30 microM stock) is diluted to a working concentration of 10 microM (2.5 microM final) in peptide buffer ( HBSS/lmM CaCl/lmM MgSO4/0.1% BSA) . This is serially dilute 1:3 in the same buffer for a total of 11 concentrations of peptide. The assay below is run and the EC50 of the CCRlO peptide is calculated. Test compounds are assayed at the EC70.
CCRlO FLIPR assay
Cell plates are removed from the incubator, inverted to "flick" out media and tapped dry on a paper towel. 25 microL Ix FLUO-4 dye/2 mM probenicid are added to each well. The plates are then incubated 30 minutes at 37°C/5% CO2, then removed and incubated 30 additional minutes at room temperature. 5 microL diluted (see below) test compound (final concentration based on 30 microL) are added to appropriate wells. The wells are mixed and incubated at room temperature for 15 minutes. The plates are then placed on FLIPR and 10 microL CCRlO peptide (30 microM stock diluted to appropriate 4x of final concentration at EC70) from a Greiner 384 well polypropylene plate are transferred. Peptide should be in columns 1-22 and peptide dilution buffer should be in columns 23 and 24 for blanks.
Plate reader data are analyzed using ActivityBase software (ID Business Solutions, Ltd). The RFU signals from the plate reader are converted to percent of control (POC) values using the formula: POC = 100*(Signal -BCTRL) ÷ (PCTRL-BCTRL)
Where Signal is the test well signal, BCTRL is the average of background (negative control) well signals on the plate and PCTRL is the average of positive control well signals on the plate.
For the concentration responsive compounds, POC as a function of test compound concentration are fitted to a 4-parameter logistic equation of the form:
Y = A + (B-A)/ [l+(x/C)D]
Where A, B, C, and D are fitted parameters (parameter B is fixed at zero POC), and x and y are the independent and dependent variables, respectively. The IC50 (50% inhibitory concentration) is determined as the inflection point parameter, C.
Compound preparation
Compound powders are diluted in vials to 10 mM in 100% DMSO. 9.7 microL 10 mM stock are added to 80 microL 100 % DMSO in column 1 of a 96 well plate. 60 microL 100% DMSO are added to remaining wells. The compound is serially dilute compound by adding 30 microL column 1 to 60 microL in column 2. Column 2 is mixed dilution is continued across the 96 well plate ending at column 10. This step is done on the Beckman FX with no tip change between columns. 16 compounds or 2x 96 well plates can be tested in Ix 384 well plate. Each 96 well plate is transferred to 1 X384 well plate. Columns 21 through 24 on the 384 well plate contain DMSO vehicle control only. Just prior to the assay 5 microL of compound from the 384 well plate is transferred to another 384 well plate containing 40 microL Ix HBSS/lmM CaCl/lmM MgSO4. This is mixed 5 microL of the diluted compound is transferred to the appropriate wells of the cell assay plate for the FLIPR assay, above.
Reagents
Ix Assay Buffer
Ix HBSS (1Ox, Invitrogen #14185-027), 10 mM HEPES pH 7.4, 0.35 g/L sodium bicarbonate, 1 mM CaCl2, 1 mM MgSO4
Fluo-4 dye/2 mM probenicid (Molecular probes Fluo-4 kit # F36206)
Resuspend dye in 100 mL Ix assay buffer and mix.
Resuspend 1 vial of probenicid with 1 mL Ix assay buffer and add to 100 mL bottle of dye
Chemotaxis Assay
Test compounds are evaluated for their ability to inhibit chemotaxis of Baf/3 cells expressing human CCRlO (hereinafter Baf/3-hCCRlO cells) in response to CCL27. Preferred compounds have IC50 < 1 micromolar in this assay.
Assay Protocol:
Test compounds are diluted (2x the final concentration) in CTX media (RPMI 1640 (Gibco- BRL #11875-093) supplemented with 0.1% BSA (Sigma #A3803)). Control solutions contain 1% DMSO in CTX media. Baf/3-hCCRlO cells are re-suspended in CTX media to a concentration of 4xlO6 cells/mL. In a 96 well plate, 100 microL the Baf/3-hCCR10 cell suspension is combined with 100 microL of the test compound solution and the plate is then incubated for 15 min at room temperature.
150 microL of a solution of the chemoattractant (2x the EC70 for CCL27) in CTX media is added to appropriate wells of a 96- well chemotaxis chamber (Neuro Probe Cat. #:116-5, 5um pore size, 5.7mm diameter size, 300 microL, 96 well plate). CTX media without chemoattractant is added to control wells. 152 microL of 2x compound solution in CTX media is added to appropriate wells. The chamber is assembled according to manufacturer's instructions using the 5 micron pore size PVP-free polycarbonate filter. Care should be taken to avoid bubbles as they will cause variation.
80 microL of the cells plus compound incubation mixture is added to upper wells of the chamber. Care is taken to avoid forming bubbles at the level of the filter. The chamber is then incubated at 37 0C for 3 hours.
The chamber is then disassembled and the filter is removed. 150 microL of media is gently removed from each well of the chemotaxis chamber. The remaining 150 microL is then mixed and 100 microL of the resulting cell suspension is transferred into a 96 well Costar 3917assay plate (Corning incorporated, cat # 3917).
The cells are measured using a CyQU ANT® NF Cell Proliferation Assay (Invitrogen, cat # C35006). 11 niL of Ix HBSS buffer is prepared by diluting 2.2 mL of 5x HBSS buffer (Component C) with 8.8 mL of deionized water. Ix dye binding solution is prepared by adding 22 microL of CyQU ANT® NF dye reagent (Component A) and 22 microL of Component C to 11 mL of Ix HBSS buffer. 100 microL of Ix dye binding solution is dispensed into wells of the 96 well Costar plate containing the cell suspensions. The plate is covered and incubated at 370C for 60 minutes. Fluorescence measurement is quantitated using a multilabel plate reader (Wallac Victor2).
Methods of Therapeutic Use
In accordance with the invention, there are provided novel methods of using the compounds of the present invention. The compounds disclosed herein effectively block the interaction of CCRlO with its ligand CCL 27. The inhibition of this interaction is an attractive means for preventing and treating a variety of diseases or conditions associated with entry and activation of T-cells into the skin or other tissues where CCRlO is found to be expressed and associated with inflammatory conditions, such as lung tissue. Thus, the compounds of the present invention are useful for the treatment of diseases and conditions including psoriasis, contact sensitivity, dermatitis, systemic sclerosis, cutaneous systemic lupus erythematosus, and allergic asthma. The compounds of the invention will also be useful for treatment of melanomas that express CCRlO.
These disorders have been well characterized in man, but also exist with a similar etiology in other mammals, and can be treated by pharmaceutical compositions of the present invention.
For therapeutic use, the compounds may be administered in any conventional dosage form in any conventional manner. Routes of administration include, but are not limited to, intravenously, intramuscularly, subcutaneously, intrasynovially, by infusion, sublingually, transdermally, orally, topically or by inhalation, tablet, capsule, caplet, liquid, solution, suspension, emulsion, lozenges, syrup, reconstitutable powder, granule, suppository and transdermal patch. Methods for preparing such dosage forms are known (see, for example, H.C. Ansel and N. G. Popovish, Pharmaceutical Dosage Forms and Drug Delivery Systems, 5th ed., Lea and Febiger (1990)).
The compounds may be administered alone or in combination with adjuvants that enhance the stability of the inhibitors, facilitate administration of pharmaceutical compositions containing them in certain embodiments, provide increased dissolution or dispersion, increase inhibitory activity, provide adjunct therapy, and the like. Advantageously, such combinations may utilize lower dosages of the active ingredient, thus reducing possible toxicity and adverse side effects. Carriers and adjuvants for use with compounds according to the present invention include, for example, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, buffer substances, water, salts or electrolytes and cellulose-based substances.

Claims

ClaimsWhat is claimed is:
1. A compound of formula (I) :
Figure imgf000108_0001
(I) wherein:
W, X, and Z are independently C or N;
Y is O, NH, or S; n is 0 or 1 ;
RMs
(a) H;
(b) Ci-galkyl, branched or unbranched, optionally partially or fully halogenated, and optionally substituted with one to two groups selected from -OH, CN, Ci- 6alkoxy, -CO2Ci-6alkyl, and -CON(Ci_3alkyl)(Ci_3alkyl),
(c) -(CH2)0-iC3-8cycloalkyl,
(d) -CH2Ar, wherein Ar is phenyl or heteroaryl selected from pyridinyl, triazolyl, pyrimidinyl, furanyl, thiazolyl, thienyl, pyrrolyl, imidazolyl, and benzofuranyl, each optionally substituted with one to two groups selected from halogen, -CN, -OH, Ci_6alkyl, Ci_6alkoxy, -CF3, -CO2Ci-6alkyl, and -CONH2, or (e) -(CH2)2θCH2Ar, wherein Ar is phenyl or heteroaryl selected from pyridinyl, triazolyl, pyrimidinyl, furanyl, thiazolyl, thienyl, pyrrolyl, imidazolyl, and benzofuranyl, each optionally substituted with one to two groups selected from halogen, -CN, -OH, C^alkyl, d_6alkoxy, -C02d_6alkyl, -C(O)NH2; or if X is C, and Y is O, R1 may form a fused dihydropyran ring with the O it is bound to and X, said dihydropyran ring optionally substituted with one or two methyl groups;
R2 is
(a) H, Ci_6alkyl, Ci_6alkoxy, hydroxyCi_6alkyl, halogen, -CN, -CO2Ci_6alkyl,
-S(0)o-2Ci_6alkyl, -NO2, -OH, -CF3, -NH2, -NH(Ci_6alkyl), -N(Ci_6alkyl)(Ci_ 6alkyl), -NHC(O)NHCi_6alkyl, -C(O)NH2, -CONH(Ci_6alkyl), -C0N(Ci_ 6alkyl)(Ci_6alkyl), or b) phenyl, pyridinyl, triazolyl, or pyrimidinyl, each optionally substituted with one or two groups selected from halogen, C^alkyl, -CN or C^alkoxy;
R3 is H, -CO2H, -(CH2)L4CO2H, -(CH2)o_iC(C1_6alkyl)(C1_6alkyl)C02H, -QCLealkylXd-e- alkyl)CO2H, -0(CH2)^CO2H, -O(CH2)0-iC(Ci_6alkyl)(Ci_6alkyl)CO2H, -OC(Ci_6alkyl)(Ci_6- alkyl)CO2H, -(CH2)0-i-tetrazol-5-yl, -C(Ci_6alkyl)(Ci_6alkyl)-tetrazol-5-yl, -0-CH(Ci_
6alkyl)-tetrazol-5-yl, -C(O)NHCH2CO2H, or -CN;
R4, R , R , and R7 are independently selected from H and Ci_6alkyl, or R4 and R may be joined, together with the carbons they are bonded to, to form a cyclopropane ring;
R8, R9, R10, and R11 are independently H, halogen, Ci_6alkyl, Ci_6alkoxy, -CN, -CO2Ci_6alkyl,
-C(O)NH2, -SO2NH2, -NO2, -OH, -NH2, -CF3, or -CH2OH; or a tautomer thereof or a salt thereof.
2. The compound of claim 1 wherein:
W, X, and Z is C; Y is O; n is 1 ; R1 Is
(a) H,
(b) Ci_8alkyl, branched or unbranched, optionally partially or fully halogenated, and optionally substituted with one to two groups selected from -OH, CN, Ci- 6alkoxy, -CO2Ci-6alkyl, -CON(Ci_3alkyl)(Ci_3alkyl),
(c) -(CH2)0-iC3-8cycloalkyl,
(d) -CH2Ar, wherein Ar is phenyl or heteroaryl selected from pyridinyl, triazolyl, pyrimidinyl, furanyl, thiazolyl, thienyl, pyrrolyl, imidazolyl, and benzofuranyl, each optionally substituted with one to two groups selected from halogen, - CN, -OH, Ci_6alkyl, Ci_6alkoxy, -CF3, -CO2Ci-6alkyl, and -CONH2, or
(e) -(CH2)2OCH2Ar wherein Ar is phenyl or heteroaryl selected from pyridinyl, triazolyl, pyrimidinyl, furanyl, thiazolyl, thienyl, pyrrolyl, imidazolyl, and benzofuranyl, each optionally substituted with one to two groups selected from halogen, -CN, -OH, C1-6alkyl, Ci_6alkoxy, -CO2Ci_6alkyl, -C(O)NH2; or
R1 may be a fused dihydropyran ring with the O it is bound to and X, said dihydropyran ring optionally substituted with one or two methyl groups;
R2 is
(a) H, Ci-βalkyl, Ci-βalkoxy, hydroxyCi_6alkyl, halogen, -CN, -CO2Ci_6alkyl, -S(O)0-2Ci_6alkyl, -NO2, -OH, -CF3, -NH2, -NH(C1-6alkyl), -N(Ci_6alkyl)(Ci_ galkyl), -NHC(O)NHC1-6alkyl, -C(O)NH2, -CONH(Ci_6alkyl), -C0N(d_ 6alkyl)(Ci_6alkyl), or
(b) phenyl, pyridinyl, triazolyl and pyrimidinyl, each optionally substituted with one or two groups selected from halogen, Ci_6alkyl, -CN or Ci_6alkoxy; R3 may be in the 4-position and is H, -CO2H, -(CH2)^4CO2H,
-(CH2)o-iC(Ci_6alkyl)(Ci_6-alkyl)C02H, -C(Ci_6alkyl)(Ci_6alkyl)C02H, -C(O)NH2,
-0(CH2)^4CO2H, -0(CH2)o-iC(Ci_6alkyl)(Ci_6alkyl)C02H, -OC(Ci_6alkyl)(Ci_6alkyl)CO2H,
-(CH2)O-I- tetrazol-5-yl, -C(C1_6alkyl)(C1_6alkyl)-tetrazol-5-yl, -O-CH(C1_6alkyl)-tetrazol-5-yl,
-C(O)NHCH2CO2H, or -CN; or
R3 may be in the 3 -position and is H or CO2H
R4, R5, R6, and R7 are independently H or methyl, or R4 and R6 may be joined, together with the carbons they are bonded to, to form a cyclopropane ring;
R8, R9, R10, and R11 are independently H, halogen, Ci_6alkyl, Ci_6alkoxy, -CN, -CO2Ci_6alkyl,
-C(O)NH2, -SO2NH2, -NO2, -OH, -NH2, -CF3, or -CH2OH; or a tautomer thereof or a salt thereof.
3. The compound of claim 1 wherein:
W, X, and Z is C:
Y is O; n is 1;
R1 is
(a) H,
(b) Ci_8alkyl, branched or unbranched, optionally partially or fully fluorinated, and optionally substituted with one to two groups selected from -OH, CN and -OCH3,
(c) -(CH2)o-iC3_8cycloalkyl, or
(d) -CH2Ar, wherein Ar is phenyl or heteroaryl selected from pyridinyl and thiazolyl, each optionally substituted with one to two groups selected from halogen, -CN, -CH3, -OCH3, -CF3, and -CONH2; or R1 may form a fused dihydropyran ring with the O it is bound to and X, said dihydropyran ring optionally substituted with one or two methyl groups; R2 is
(a) -Cl, -Br, -CN, -CO2C1_6alkyl, -NO2, -OH, -CF3, -NH2; or
(b) phenyl, pyridinyl, or pyrimidinyl;
R3 may be in the 4-position and is H, -CO2H, -(CH2)^4CO2H, -(CH2)o-iC(CH3)(CH3)C02H,
-C(CH3)(CH3)CO2H, -0(CH2)1_4C02H, -0(CH2)o-iC(Ci_6alkyl)(Ci_6alkyl)C02H,
-OC(Ci_6alkyl)(Ci_6alkyl)CO2H, -(CH2)0-itetrazol-5-yl, or -C(CH3)(CH3)tetrazol-5-yl; or
R3 may be in the 3-position and is H or CO2H;
R4, R5, R6, and R7 are independently H or methyl, or R4 and R6 may be joined, together with the carbons they are bonded to, to form a cyclopropane ring;
R8, R9, R10, and R11 are independently H, F, Cl, CH3, -OCH3, -CN, -NO2, -NH2, or -CF3; or a tautomer thereof or a salt thereof.
4. The compound of claim 1 wherein:
W, X, and Z are C:
Y is O; n is 1;
R1 is
(a) Ci_8alkyl, branched or unbranched, optionally partially or fully fluorinated;
(b) -(CH2)o-iC3_8cycloalkyl, or
(c) -CH2Ar, wherein Ar is phenyl or heteroaryl selected from pyridinyl and thiazolyl, each optionally substituted with one to two groups selected from F, - CN, -CH3, -OCH3, and -CF3,
R2 is Cl or Br; R3 is in the 4-position and is H, -CO2H, -(CH2)L2CO2H, -(CH2)o-iC(CH3)(CH3)C02H,
-C(CH3)(CH3)CO2H, -0(CH2)L4CO2H, -0(CH2)o-iC(Ci_6alkyl)(Ci_6alkyl)C02H,
-OC(Ci_6alkyl)(Ci_6alkyl)CO2H, -(CH2)o-1tetrazol-5-yl, or -C(CH3)(CH3)tetrazol-5-yl;
R4, R5, R6, and R7 are independently H or methyl, or R4 and R6 may be joined, together with the carbons they are bonded to, to form a cyclopropane ring;
R8, R9, R10, and R11 are independently H, F, Cl, CH3, -OCH3, -CN, -NO2, or -NH2; or a tautomer thereof or a salt thereof.
5. A compound selected from the group consisting of:
(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-4-yl)acetic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -2,5 -difluorobiphenyl-4-yl)oxy] -2- methylpropanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-3-chlorobiphenyl-4-carboxylic acid;
2- [(4'- { 2- [(2-butoxy-5-chlorobenzoyl)amino] ethyl } -3-fluorobiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2- [(4'- { 2- [(5 -chloro-2-methoxybenzoyl)amino] ethyl } -2,3 -dimethylbiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl }biphenyl-4-yl)oxy] -2-methylpropanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2-nitrobiphenyl-4-carboxylic acid;
2- { [4'-(2- { [2-(benzyloxy)-5 -chlorobenzoyl] amino } ethyl)biphenyl-4-yl] oxy } -2- methylpropanoic acid;
2-(benzyloxy)-5-chloro-N-{2-[4'-(lH-tetrazol-5-yl)biphenyl-4-yl]ethyl}benzamide;
2- [(4'- { 2- [(5 -chloro-2-methoxybenzoyl)amino] ethyl } -2,5 -difluorobiphenyl-4-yl)oxy] -2- methylpropanoic acid; 4'- { 2- [(2-butoxy-5-chlorobenzoyl)amino]ethyl } -2-chlorobiphenyl-4-carboxylic acid;
2-[(4'-{2-[(5-chloro-2-propoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2-methylpropanoic acid;
4'-[2-({5-chloro-2-[(3-fluorobenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
4'-[2-({5-chloro-2-[(3-methoxybenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -2-fluorobiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2-butoxy-5-chloro-N-(2-{4'-[l-methyl-l-(lH-tetrazol-5-yl)ethyl]biphenyl-4- yl } ethyl)benzamide ;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-3-fluorobiphenyl-4-carboxylic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -3 -methoxybiphenyl-4-yl)oxy] -2- methylpropanoic acid;
4'-(2-{ [5-chloro-2-(hexyloxy)benzoyl] amino }ethyl)biphenyl-4-carboxylic acid;
2-[(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-3-yl)oxy]-2-methylpropanoic acid;
2-{ [4'-(2-{ [5-chloro-2-(4,4,4-trifluorobutoxy)benzoyl]amino}ethyl)biphenyl-4-yl]oxy}-2- methylpropanoic acid;
4'-(2-{ [5-chloro-2-(pentyloxy)benzoyl] amino }ethyl)biphenyl-4-carboxylic acid;
4'-(2-{ [5-chloro-2-(heptyloxy)benzoyl] amino }ethyl)biphenyl-4-carboxylic acid;
4'-[2-({5-chloro-2-[(4-methylpentyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
2-[(2,3-dichloro-4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2- methylpropanoic acid;
2-butoxy-5-chloro-N-{2-[4'-(lH-tetrazol-5-ylmethyl)biphenyl-4-yl]ethyl}benzamide;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2-methylbiphenyl-4-carboxylic acid; 2-[(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-3-methylbiphenyl-4-yl)oxy]-2- methylpropanoic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -2-methylbiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2-[(2-chloro-4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-[2-({5-chloro-2-[(3-methylbenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
2- [(4'- { 2- [(5 -chloro-2-methoxybenzoyl)amino] ethyl } -2-methylbiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -2,3 -dimethylbiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2- [(4'- { 2- [(5-chloro-2-methoxybenzoyl)amino]ethyl } -3-methylbiphenyl-4-yl)oxy] -2- methylpropanoic acid;
4'-[2-({5-chloro-2-[(4-methylbenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
4'-[2-({5-chloro-2-[(6-hydroxyhexyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
2- [(4'- { 2- [(5 -chloro-2-methoxybenzoyl)amino] ethyl } -2-fluorobiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -2-chlorobiphenyl-4-yl)oxy] -2- methylpropanoic acid;
2-[(4'-{2-[(5-chloro-2-isopropoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-3-methoxybiphenyl-4-carboxylic acid;
2-{ [4'-(2-{ [5-chloro-2-(3,3,4,4,4-pentafluorobutoxy)benzoyl]amino}ethyl)biphenyl-4- yl]oxy}-2-methylpropanoic acid; 2-[(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}-2,5-dimethylbiphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-[2-({2-[2-(benzyloxy)ethoxy]-5-chlorobenzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
2-{ [4'-(2-{ [5-chloro-2-(4-fluorobutoxy)benzoyl]amino}ethyl)biphenyl-4-yl]oxy}-2- methylpropanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-5-fluorobiphenyl-3-carboxylic acid
2-[(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}-3-fluorobiphenyl-4-yl)oxy]-2- methylpropanoic acid;
3-(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-4-yl)-2,2-dimethylpropanoic acid;
4'-{2-[(5-bromo-2-butoxybenzoyl)amino]ethyl}biphenyl-4-carboxylic acid;
2-[(4'-{2-[(5-chloro-2-ethoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2-methylpropanoic acid;
4'-(2-{ [5-chloro-2-(4,4,4-trifluorobutoxy)benzoyl] amino }ethyl)biphenyl-4-carboxylic acid;
3 -(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl }biphenyl-4-yl)propanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-5-nitrobiphenyl-3-carboxylic acid;
2-(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-4-yl)-2-methylpropanoic acid;
2-[(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}-3'-fluorobiphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-[2-({5-chloro-2-[(4-fluorobenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
4'-[2-({5-chloro-2-[(3,4-difluorobenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid;
(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-3-nitrobiphenyl-4-yl)acetic acid;
4'-(2-{ [5-chloro-2-(3-methylbutoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid; 4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-3-carboxylic acid; 4'-[2-({5-chloro-2-[(2-fluorobenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid; 4'- { 2- [(5 -chloro-2- { [3 -(trifluoromethyl)benzyl] oxy }benzoyl)amino] ethyl } biphenyl-4- carboxylic acid;
4'-(2-{ [5-chloro-2-(cyclobutylmethoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid; 4'-(2-{ [(5-bromo-2,2-dimethyl-2,3-dihydro-l-benzofuran-7-yl)carbonyl]amino}ethyl)- biphenyl-4-carboxylic acid;
5-chloro-N-[2-(2'-cyanobiphenyl-4-yl)ethyl]-2-hydroxybenzamide;
3-(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)-2,2-dimethylpropanoic acid;
2-chloro-4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-carboxylic acid; 2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -3 -cyanobiphenyl-4-yl)oxy] -2- methylpropanoic acid;
4'-[2-({5-chloro-2-[(3-cyanobenzyl)oxy]benzoyl}amino)ethyl]biphenyl-4-carboxylic acid; 4'-(2-{ [5-chloro-2-(cyclohexylmethoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid; 4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-6-fluorobiphenyl-3-carboxylic acid; 4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]-l-methylethyl}biphenyl-4-carboxylic acid; 4'-(2-{ [5-chloro-2-(3,3,4,4,4-pentafluorobutoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid;
4'-(2-{ [(2-butoxy-5-chloropyridin-3-yl)carbonyl]amino}ethyl)biphenyl-4-carboxylic acid; 2-[(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2-methylpropanoic acid; 2-butoxy-5-chloro-N-(2-{4-[6-(lH-tetrazol-5-yl)pyridin-3-yl]phenyl}ethyl)benzamide; 4'-(2-{ [5-chloro-2-(pyridin-2-ylmethoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid; 4'-{2-[(5-chloro-2-{ [2-(trifluoromethyl)benzyl]oxy}benzoyl)amino]ethyl}biphenyl-4- carboxylic acid;
2-[(3-chloro-4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2-cyanobiphenyl-4-carboxylic acid; 4'-{2-[(5-chloro-2-ethoxybenzoyl)amino]ethyl}biphenyl-4-carboxylic acid; 4 '-(2- { [5-chloro-2-(l -methylbutoxy)benzoyl] amino } ethyl)biphenyl-4-carboxylic acid; 3-chloro-4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-carboxylic acid; 2-[(2',6'-dichloro-4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-{2-[(5-chloro-2-isobutoxybenzoyl)amino]ethyl}biphenyl-4-carboxylic acid; 4'-{2-[(5-chloro-2-propoxybenzoyl)amino]ethyl}biphenyl-4-carboxylic acid; 4'-[2-({5-chloro-2-[(4-methyl-l,3-thiazol-2-yl)methoxy]benzoyl}amino)ethyl]biphenyl-4- carboxylic acid;
2-[(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2,5-dimethylbiphenyl-4-yl)oxy]-2- methylpropanoic acid;
4'-(2-{ [5-chloro-2-(4-fluorobutoxy)benzoyl] amino }ethyl)biphenyl-4-carboxylic acid; 2- [(4'- { 2- [(2-butoxy-5 -chlorobenzoyl)amino] ethyl } -3 -chlorobiphenyl-4-yl)oxy] -2- methylpropanoic acid;
4'-{2-[(5-chloro-2-hydroxybenzoyl)amino]ethyl}biphenyl-2-carboxamide; (4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)acetic acid; 4'-(2-{ [5-chloro-2-(cyclopropylmethoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid; 4'-(2-{ [5-chloro-2-(pyridin-3-ylmethoxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid; 4'- { 2- [(2-butoxy-5-chlorobenzoyl)amino] -1,1 -dimethylethyl }biphenyl-4-carboxylic acid; 4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]propyl}biphenyl-4-carboxylic acid; 4'-(2-{ [(5-bromo-2,3-dihydro-l-benzofuran-7-yl)carbonyl]amino}ethyl)biphenyl-4- carboxylic acid;
2-{ [4'-(2-{ [5-chloro-2-(3,3,3-trifluoropropoxy)benzoyl]amino}ethyl)biphenyl-4-yl]oxy}-2- methylpropanoic acid;
2-amino-4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-4-carboxylic acid; 2-[(4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}-2,6-dimethylbiphenyl-4-yl)oxy]-2- methylpropanoic acid;
2-(4'-{2-[(5-chloro-2-methoxybenzoyl)amino]ethyl}biphenyl-4-yl)-2-methylpropanoic acid; 4'-{2-[(2-butoxy-5-pyridin-3-ylbenzoyl)amino]ethyl}biphenyl-4-carboxylic acid; 4'-(2-{ [5-chloro-2-(cyclobutyloxy)benzoyl]amino}ethyl)biphenyl-4-carboxylic acid; 4'-{2-[(2-butoxy-5-chlorobenzoyl)amino]ethyl}biphenyl-4-carboxylic acid; 2-(4-{5-[2-(2-butoxy-5-chloro-benzoylamino)-ethyl]-pyridin-2-yl}-phenoxy)-2-methyl- propionic acid;
2-{4'-[2-(2-butoxy-5-chloro-benzoylamino)-ethyl]-3'-ethyl-biphenyl-4-yloxy}-2-methyl- propionic acid;
2-{3',5'-Dichloro-4'-[2-(5-chloro-2-methoxy-benzoylamino)-ethyl]-biphenyl-4-yloxy}-2- methyl-propionic acid; and a tautomer thereof or a salt thereof:
6. A pharmaceutical composition comprising a compound of any one of claims 1 to 5 and a pharmaceutically acceptable carrier.
7. A method of using any one of the compounds of any of claims 1 to 5 for the treatment of psoriasis, contact sensitivity, dermatitis, systemic sclerosis, cutaneous systemic lupus erythematosus, and allergic asthma.
8. A method of using the pharmaceutical composition of claim 6 for the treatment of psoriasis, contact sensitivity, dermatitis, systemic sclerosis, cutaneous systemic lupus erythematosus, and allergic asthma.
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