WO2007021982A2 - Modulators of cystic fibrosis transmembrane conductance regulator - Google Patents
Modulators of cystic fibrosis transmembrane conductance regulator Download PDFInfo
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- WO2007021982A2 WO2007021982A2 PCT/US2006/031457 US2006031457W WO2007021982A2 WO 2007021982 A2 WO2007021982 A2 WO 2007021982A2 US 2006031457 W US2006031457 W US 2006031457W WO 2007021982 A2 WO2007021982 A2 WO 2007021982A2
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- 0 *c1c(*)cc(CC#N)cc1 Chemical compound *c1c(*)cc(CC#N)cc1 0.000 description 6
- BMKXGOBYGCGOAH-IPQOISQHSA-N CC(C)(C)[S@@](N[C@@H](c1c(C)nc(N)[s]1)c(cccc1)c1Cl)=O Chemical compound CC(C)(C)[S@@](N[C@@H](c1c(C)nc(N)[s]1)c(cccc1)c1Cl)=O BMKXGOBYGCGOAH-IPQOISQHSA-N 0.000 description 1
- SRDFKFIYSZNBFO-QGFSVNGOSA-N CC(C)(C)[S@@](N[C@@H](c1cnc(NC(C2(CC2)c2ccc3OCOc3c2)=O)[s]1)c1ccccc1Cl)=O Chemical compound CC(C)(C)[S@@](N[C@@H](c1cnc(NC(C2(CC2)c2ccc3OCOc3c2)=O)[s]1)c1ccccc1Cl)=O SRDFKFIYSZNBFO-QGFSVNGOSA-N 0.000 description 1
- BMKXGOBYGCGOAH-YTEVENLXSA-N CC(C)(C)[S@@](N[C@H](c1c(C)nc(N)[s]1)c(cccc1)c1Cl)=O Chemical compound CC(C)(C)[S@@](N[C@H](c1c(C)nc(N)[s]1)c(cccc1)c1Cl)=O BMKXGOBYGCGOAH-YTEVENLXSA-N 0.000 description 1
- ARNOSENRPAMSLZ-VERVWZFWSA-N CC(C)(C)[S@](N[C@@H](c1cnc(N)[s]1)c(ccc(F)c1)c1Cl)=O Chemical compound CC(C)(C)[S@](N[C@@H](c1cnc(N)[s]1)c(ccc(F)c1)c1Cl)=O ARNOSENRPAMSLZ-VERVWZFWSA-N 0.000 description 1
- JWNIHPKSGUQKQI-WPLJPSAOSA-N CC(C)(C)[S@](N[C@@H](c1cnc(NC(C2(CC2)c(cc2)cc(O3)c2OC3(F)F)=O)[s]1)c(ccc(F)c1)c1Cl)=O Chemical compound CC(C)(C)[S@](N[C@@H](c1cnc(NC(C2(CC2)c(cc2)cc(O3)c2OC3(F)F)=O)[s]1)c(ccc(F)c1)c1Cl)=O JWNIHPKSGUQKQI-WPLJPSAOSA-N 0.000 description 1
- BMKBJOKAKBZGKN-PQZOHRHNSA-N CC(C)(C)[S@](N[C@@H](c1cnc(NC(C2(CC2)c2ccc3OCOc3c2)=O)[s]1)c(ccc(F)c1)c1Cl)=O Chemical compound CC(C)(C)[S@](N[C@@H](c1cnc(NC(C2(CC2)c2ccc3OCOc3c2)=O)[s]1)c(ccc(F)c1)c1Cl)=O BMKBJOKAKBZGKN-PQZOHRHNSA-N 0.000 description 1
- AGLOYWZGOCVBSV-DDWIDDIQSA-N CC(C)[C@@H](CC[C@H](C)C1)[C@H]1OC(O[C@H](CC1)CN1[C@@H](c1cnc(NC(C2(CC2)c2ccc3OCOc3c2)=O)[s]1)c1ccccc1Cl)=O Chemical compound CC(C)[C@@H](CC[C@H](C)C1)[C@H]1OC(O[C@H](CC1)CN1[C@@H](c1cnc(NC(C2(CC2)c2ccc3OCOc3c2)=O)[s]1)c1ccccc1Cl)=O AGLOYWZGOCVBSV-DDWIDDIQSA-N 0.000 description 1
- AGLOYWZGOCVBSV-MSVCNYQESA-N CC(C)[C@@H](CC[C@H](C)C1)[C@H]1OC(O[C@H](CC1)CN1[C@H](c1cnc(NC(C2(CC2)c(cc2)cc3c2OCO3)=O)[s]1)c(cccc1)c1Cl)=O Chemical compound CC(C)[C@@H](CC[C@H](C)C1)[C@H]1OC(O[C@H](CC1)CN1[C@H](c1cnc(NC(C2(CC2)c(cc2)cc3c2OCO3)=O)[s]1)c(cccc1)c1Cl)=O AGLOYWZGOCVBSV-MSVCNYQESA-N 0.000 description 1
- ZZGLFLPQDVQDHO-SFHVURJKSA-N N[C@H](c1cnc(NC(C2(CC2)c(cc2)cc3c2OCO3)=O)[s]1)c1ccccc1Cl Chemical compound N[C@H](c1cnc(NC(C2(CC2)c(cc2)cc3c2OCO3)=O)[s]1)c1ccccc1Cl ZZGLFLPQDVQDHO-SFHVURJKSA-N 0.000 description 1
- IHVPTLJZIDYTPZ-RBFZIWAESA-N O[C@H](CC1)CN1C(c1cnc(NC(C2(CC2)c(cc2O3)ccc2OC3(F)F)=O)[s]1)c(ccc(F)c1)c1Cl Chemical compound O[C@H](CC1)CN1C(c1cnc(NC(C2(CC2)c(cc2O3)ccc2OC3(F)F)=O)[s]1)c(ccc(F)c1)c1Cl IHVPTLJZIDYTPZ-RBFZIWAESA-N 0.000 description 1
- OUGLISSDQZYBGK-OPAMFIHVSA-N O[C@H](CC1)CN1[C@@H](c1cnc(NC(C2(CC2)c2ccc3OCOc3c2)=O)[s]1)c1ccccc1Cl Chemical compound O[C@H](CC1)CN1[C@@H](c1cnc(NC(C2(CC2)c2ccc3OCOc3c2)=O)[s]1)c1ccccc1Cl OUGLISSDQZYBGK-OPAMFIHVSA-N 0.000 description 1
- OUGLISSDQZYBGK-ZHRRBRCNSA-N O[C@H](CC1)CN1[C@H](c1cnc(NC(C2(CC2)c(cc2)cc3c2OCO3)=O)[s]1)c1ccccc1Cl Chemical compound O[C@H](CC1)CN1[C@H](c1cnc(NC(C2(CC2)c(cc2)cc3c2OCO3)=O)[s]1)c1ccccc1Cl OUGLISSDQZYBGK-ZHRRBRCNSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
- C07D417/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing three or more hetero rings
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- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/425—Thiazoles
- A61K31/427—Thiazoles not condensed and containing further heterocyclic rings
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Definitions
- the present invention relates to modulators of cystic fibrosis Transmembrane Conductance Regulator ("CFTR"), compositions thereof, and methods therewith.
- CFTR cystic fibrosis Transmembrane Conductance Regulator
- the present invention also relates to methods of treating CFTR mediated diseases using such modulators.
- ABC transporters are a family of membrane transporter proteins that regulate the transport of a wide variety of pharmacological agents, potentially toxic drugs, and xenobiotics, as well as anions.
- ABC transporters are homologous membrane proteins that bind and use cellular adenosine triphosphate (ATP) for their specific activities.
- Some of these transporters were discovered as multidrug resistance proteins (like the MDRl-P glycoprotein, or the multidrug resistance protein, MRPl), defending malignant cancer cells against chemotherapeutic agents.
- MRPl multidrug resistance protein
- 48 ABC Transporters have been identified and grouped into 7 families based on their sequence identity and function.
- ABC transporters regulate a variety of important physiological roles within the body and provide defense against harmful environmental compounds. Because of this, they represent important potential drug targets for the treatment of diseases associated with defects in the transporter, prevention of drug transport out of the target cell, and intervention in other diseases in which modulation of ABC transporter activity may be beneficial.
- CFTR cAMP/ ATP -mediated anion channel
- CFTR is expressed in a variety of cells types, including absorptive and secretory epithelia cells, where it regulates anion flux across the membrane, as well as the activity of other ion channels and proteins. In epithelia cells, normal functioning of CFTR is critical for the maintenance of electrolyte transport throughout the body, including respiratory and digestive tissue.
- CFTR is composed of approximately 1480 amino acids that encode a protein made up of a tandem repeate of transmembrane domains, each containing six transmembrane helices and a nucleotide binding domain. The two transmembrane domains are linked by a large, polar, regulatory (R)-domain with multiple phosphorylation sites that regulate channel activity and cellular trafficking.
- cystic fibrosis hi patients with cystic fibrosis, mutations in CFTR endogenously expressed in respiratory epithelia leads to reduced apical anion secretion causing an imbalance in ion and fluid transport. The resulting decrease in anion transport contributes to enhanced mucus accumulation in the lung and the accompanying microbial infections that ultimately cause death in cystic fibrosis patients, hi addition to respiratory disease, cystic fibrosis patients typically suffer from gastrointestinal problems and pancreatic insufficiency that, if left untreated, results in death, hi addition, the majority of males with cystic fibrosis are infertile and fertility is decreased among females with cystic fibrosis.
- cystic fibrosis associated gene In contrast to the severe effects of two copies of the cystic fibrosis associated gene, individuals with a single copy of the cystic fibrosis associated gene exhibit increased resistance to cholera and to dehydration resulting from diarrhea - perhaps explaining the relatively high frequency of the cystic fibrosis gene within the population.
- the most prevalent mutation is a deletion of phenylalanine at position 508 of the CFTR amino acid sequence, and is commonly referred to as ⁇ F508-CFTR. This mutation occurs in approximately 70% of the cases of cystic fibrosis and is associated with a severe disease .
- CFTR transports a variety of molecules in addition to anions
- this role represents one element in an important mechanism of transporting ions and water across the epithelium.
- the other elements include the epithelial Na + channel, ENaC, Na + /2C17K + co-transporter, Na + -K + - ATPase pump and the basolateral membrane K + channels, that are responsible for the uptake of chloride into the cell.
- CFTR Arrangement of Na + /2C17K + co-transporter, Na + -K + -ATPase pump and the basolateral membrane K + channels on the basolateral surface and CFTR on the luminal side coordinate the secretion of chloride via CFTR on the luminal side. Because water is probably never actively transported itself, its flow across epithelia depends on tiny transepithelial osmotic gradients generated by the bulk flow of sodium and chloride. [0011] In addition to cystic fibrosis, modulation of CFTR activity may be beneficial for other diseases not directly caused by mutations in CFTR, such as secretory diseases and other protein folding diseases mediated by CFTR. These include, but are not limited to, chronic obstructive pulmonary disease (COPD), dry eye disease, and Sjogren's Syndrome.
- COPD chronic obstructive pulmonary disease
- COPD chronic obstructive pulmonary disease
- dry eye disease dry eye disease
- Sjogren's Syndrome Sjogren's Syndrome.
- COPD is characterized by airflow limitation that is progressive and not fully reversible.
- the airflow limitation is due to mucus hypersecretion, emphysema, and bronchiolitis.
- Activators of mutant or wild-type CFTR offer a potential treatment of mucus hypersecretion and impaired mucociliary clearance that is common in COPD.
- increasing anion secretion across CFTR may facilitate fluid transport into the airway surface liquid to hydrate the mucus and optimized periciliary fluid viscosity. This would lead to enhanced mucociliary clearance and a reduction in the symptoms associated with COPD.
- Dry eye disease is characterized by a decrease in tear aqueous production and abnormal tear film lipid, protein and mucin profiles.
- Sjogrens's syndrome is an autoimmune disease in which the immune system attacks moisture-producing glands throughout the body, including the eye, mouth, skin, respiratory tissue, liver, vagina, and gut. Symptoms, include, dry eye, mouth, and vagina, as well as lung disease.
- the disease is also associated with rheumatoid arthritis, systemic lupus, systemic sclerosis, and polymypositis/dermatomyositis. Defective protein trafficking is believed to cause the disease, for which treatment options are limited. Modulators of CFTR activity may hydrate the various organs afflicted by the disease and help to elevate the associated symptoms.
- the diseases associated with the first class of ER malfunction are cystic fibrosis (due to misfolded ⁇ F508-CFTR as discussed above), hereditary emphysema (due to al -antitrypsin; non Piz variants), hereditary hemochromatosis, coagulation-fibrinolysis deficiencies, such as protein C deficiency, Type 1 hereditary angioedema, lipid processing deficiencies, such as familial hypercholesterolemia, Type 1 chylomicronemia, abetalipoproteinemia, lysosomal storage diseases, such as I-cell disease/pseudo-Hurler, mucopolysaccharidoses (due to lysosomal processing enzymes), Sandhof/Tay-Sachs (due to ⁇ -hexosaminidase), Crigler-Najjar type II (due to UDP-glucuronyl- sialyc-transferase), polyendoc
- the diseases associated with the latter class of ER malfunction are glycanosis CDG type 1, hereditary emphysema (due to ⁇ l -antitrypsin (PiZ variant), congenital hyperthyroidism, osteogenesis imperfecta (due to Type I, II, IV procollagen), hereditary hypofibrinogenemia (due to fibrinogen), ACT deficiency (due to ⁇ l-antichymotrypsin), diabetes insipidus (DI), neurophyseal DI (due to vasopvessin hormone/V2-receptor), neprogenic DI (due to aquaporin II), Charcot-Marie Tooth syndrome (due to peripheral myelin protein 22), Perlizaeus-Merzbacher disease, neurodegenerative diseases such as Alzheimer's disease ( due to ⁇ APP and presenilins), Parkinson's disease, amyotrophic lateral sclerosis, progressive supranuclear plasy,
- CFTR modulators may be beneficial for the treatment of secretory diarrheas, in which epithelial water transport is dramatically increased as a result of secretagogue activated chloride transport.
- the mechanism involves elevation of cAMP and stimulation of CFTR.
- Acute and chronic diarrheas represent a major medical problem in many areas of the world. Diarrhea is both a significant factor in malnutrition and the leading cause of death (5,000,000 deaths/year) in children less than five years old.
- Diarrhea in barn animals and pets such as cows, pigs and horses, sheep, goats, cats and dogs, also known as scours, is a major cause of death in these animals. Diarrhea can result from any major transition, such as weaning or physical movement, as well as in response to a variety of bacterial or viral infections and generally occurs within the first few hours of the animal's life.
- ETEC enterotoxogenic E-coli
- Common viral causes of diarrhea include rotavirus and coronavirus.
- Other infectious agents include Cryptosporidium, giardia lamblia, and salmonella, among others.
- Symptoms of rotaviral infection include excretion of watery feces, dehydration and weakness. Coronavirus causes a more severe illness in the newborn animals, and has a higher mortality rate than rotaviral infection. Often, however, a young animal may be infected with more than one virus or with a combination of viral and bacterial microorganisms at one time. This dramatically increases the severity of the disease. IDU21] Accordingly, there is a need for modulators of CFTR activity, and compositions thereof, that can be used to modulate the activity of the CFTR in the cell membrane of a mammal.
- compositions are useful for treating or lessening the severity of a variety of diseases, disorders, or conditions, including, but not limited to, cystic fibrosis, hereditary emphysema, hereditary hemochromatosis, coagulation-fibrinolysis deficiencies, such as protein C deficiency, Type 1 hereditary angioedema, lipid processing deficiencies, such as familial hypercholesterolemia, Type 1 chylomicronemia, abetalipoproteinemia, lysosomal storage diseases, such as I-cell disease/pseudo-Hurler, mucopolysaccharidoses, Sandhof/Tay-Sachs, Crigler-Najjar type II, polyendocrinopathy/hyperinsulemia, diabetes mellitus, laron dwarfism, myleoperoxidase deficiency, primary hypoparathyroidism, melanoma, glycanosis CDG type 1,
- each R x is independently hydrogen, halo, CF 3 , C1-C4 alkyl, or -OC1-C4 alkyl; provided that both R x are not simultaneously hydrogen; or the two R x , taken together form ring (a):
- X is CH 2 , CF 2 , CH 2 -CH 2 , or CF 2 -CF 2 ;
- ring A is 3-7 membered monocyclic cycloalkyl ring;
- R' is hydrogen or C1-C6 aliphatic, wherein up to two carbon units of said aliphatic are optionally and independently replaced by -CO-, -CS-, -COCO-, -CONR-, -CONRNR-, -CO 2 -, -OCO-, -NRCO 2 -, -0-, -NRCONR-, -OCONR-, -NRNR, -NRNRCO-, -NRCO-, -S-, -SO, -SO 2 -, -NR-, -SO 2 NR-, NRSO 2 -, or -NRSO 2 NR-;
- R is hydrogen or C1-C6 aliphatic; Z is an electron withdrawing substituent; and q is 0-3.
- CFTR cystic fibrosis transmembrane conductance regulator or a mutation thereof capable of regulator activity, including, but not limited to, ⁇ F508 CFTR and G551D CFTR (see, e.g., https://www.genet.sickkids.on.ca/cftr/. for CFTR mutations).
- modulating means increasing or decreasing by a measurable amount.
- correction means increasing the number of CFTR in a membrane of a cell.
- potentiator means a compound that increases the gating activity of CFTR in a membrane of a cell.
- electronegative substituent means an atom or a group that is electronegative relative to hydrogen. See, e.g., “Advanced Organic Chemistry: Reactions, Mechanisms, and Structure," Jerry March, 4 th Ed., John Wiley & Sons (1992), e.g., pp. 14-16, 18-19, etc. Exemplary such substituents include halo, CN, COOH, CF 3 , etc.
- aliphatic or "aliphatic group” by itself, as used herein, means a straight-chain (i.e., unbranched) or branched, substituted or unsubstituted hydrocarbon chain that is completely saturated or that contains one or more units of unsaturation, or a monocyclic hydrocarbon or bicyclic hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic (also referred to herein as “carbocycle” "cycloaliphatic” or “cycloalkyl”), that has a single point of attachment to the rest of the molecule.
- aliphatic groups contain 1-20 aliphatic carbon atoms.
- aliphatic groups contain 1-10 aliphatic carbon atoms. In other embodiments, aliphatic groups contain 1-8 aliphatic carbon atoms, hi still other embodiments, aliphatic groups contain 1-6 aliphatic carbon atoms, and in yet other embodiments aliphatic groups contain 1-4 aliphatic carbon atoms.
- cycloaliphatic refers to a monocyclic C3-C8 hydrocarbon or bicyclic C8-C12 hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic, that has a single point of attachment to the rest of the molecule wherein any individual ring in said bicyclic ring system has 3-7 members.
- Suitable aliphatic groups include, but are not limited to, linear or branched, substituted or unsubstituted alkyl, alkenyl, alkynyl groups and hybrids thereof such as (cycloalkyl)alkyl, (cycloalkenyl)alkyl or (cycloalkyl)alkenyl.
- structures depicted herein are also meant to include all isomeric (e.g., enantiomeric, diastereomeric, and geometric (or conformational)) forms of the structure; for example, the R and S configurations for each asymmetric center, (Z) and (E) double bond isomers, and (Z) and (E) conformational isomers, including mixtures containing an excess of one enantiomer relative to the other enantiomer or an excess of one diastereomer relative to another.
- single stereochemical isomers as well as enantiomeric, diastereomeric, and geometric (or conformational) mixtures of the present compounds are within the scope of the invention.
- each R x is independently hydrogen, halo, or CF 3 ; provided that both R x are not simultaneously hydrogen.
- one R x is hydrogen and the other R x is halo or CF 3 .
- both R x are halo.
- X is CH 2 . In other embodiments, X is CF 2 . Or, X is CH 2 -CH 2 . In certain other embodiments, X is CF 2 -CF 2 ;
- ring A is cyclopropyl, cyclopentyl, or cyclohexyl. In another embodiment, ring A is cyclopropyl or cyclopentyl. In certain embodiments, ring A is cyclopropyl.
- R is hydrogen. Or, R is C1-C6 alkyl. Exemplary R includes methyl, ethyl, or propyl.
- R' is hydrogen. Or, R' is Cl -C6 alkyl. Exemplary R' include methyl, ethyl, propyl, or C(O)Me.
- R AA and R BB taken together, form a pyrrolidinyl with an OH substituent.
- Z is selected from halo, CF 3 , or difluoromethylenedioxy.
- q is 0. Or, q is 1-2. hi certain embodiments, q is 1. Or, q is 2.
- compounds of formula I or formula II comprise one or more, and preferably all, of the following features: the two R x are taken together to form ring (a);
- X is CH 2 ; ring A is cyclopropyl;
- R' is hydrogen; q is 1 or 2;
- Z is halo, CF 3 , or difluoromethylenedioxy.
- compounds of formula I or formula II comprise one or more, and preferably all, of the following features: the two R x are taken together to form ring (a);
- X is CH 2 ;
- R is hydrogen; ring A is cyclopropyl;
- R' is hydrogen; q is 1 or 2;
- compounds of formula I or formula II comprise one or more, and preferably all, of the following features: the two R x are taken together to form ring (a);
- ring A is cyclopropyl
- R' is hydrogen; q is 1 or 2;
- Z is halo, CF 3 , or difluoromethylenedioxy.
- compounds of formula I or formula II comprise one or more, and preferably all, of the following features: the two R x are taken together to form ring (a);
- X is CF 2 ;
- R is hydrogen; ring A is cyclopropyl;
- R' is hydrogen; q is 1 or 2;
- Z is halo, CF 3 , or difluoromethylenedioxy.
- R AA and R BB taken together with the nitrogen atom, form the following ring (ii):
- the present invention provides intermediates having formula I' or formula II':
- R , R, ring A, Z, and q are as defined above; L is a linker selected from C(O) , SO 2 ; p is 0 or 1 ; and CA is a suitable chiral auxiliary.
- chiral auxiliary as used herein means an asymmetric molecule or molecular fragment that is used to achieve the chemical resolution of a racemic or diastereomeric mixture.
- Such chiral auxiliaries may possess one chiral center such as methylbenzylamine or several chiral centers such as menthol.
- the purpose of a chiral auxiliary, once built into the starting material, is to allow simple separation of the resulting diastereomeric mixture. See, for example, J. Jacques et al., “Enantiomers, Racemates And Resolutions, ' " pp. 251-369, John Wiley & Sons, New York (1981); E. L. Eliel & S. H. Wilen, "Stereochemistry of Organic Compounds:' pp. 868-870, John Wiley & Sons (1994).
- Suitable chiral auxiliaries useful in the present invention include those that are amenable to attachment to the linker L above (i.e., p is 1) or directly to the oxygen atom (i.e., p is 0). Exemplary such chiral auxiliaries are found in, e.g., E. L. Eliel & S. H. Wilen, ibid, pp. 337-340.
- CA, L, p, and the oxygen atom linked thereto, taken together is (+)-10-camphorsulfonate, (IS, 4i?)-(-)- ⁇ -camphanic ester, (Ii?, 2S, 5i?)-(-) mentholcarbonate, (IS, 2R, 55)-(+)-mentholcarbonate, (Ii?, 2i?)-l ⁇ phenyl-2-cyclopropylester, or (3i?)-tetrahydrofuran-3 -carbonate.
- the present invention provides a method for producing a compound of formula I or formula II:
- each R >x A is independently hydrogen, halo, CF 3 , C1-C4 alkyl, or -OC1-C4 alkyl; provided that both R x are not simultaneously hydrogen; or the two R >x ⁇ , taken together form ring (a):
- X is CH 2 , CF 2 , CH 2 -CH 2 , or CF 2 -CF 2 ;
- ring A is 3-7 membered monocyclic cycloalkyl ring;
- R' is hydrogen or C1-C6 aliphatic, wherein up to two carbon units of said aliphatic are optionally and independently replaced by -CO-, -CS-, -COCO-, -CONR-, -CONRNR-, -CO 2 -, -OCO-, -NRCO 2 -, -0-, -NRCONR-, -OCONR-, -NRNR, -NRNRCO-, -NRCO-, -S-, -SO, -SO 2 -, -NR-, -SO 2 NR-, NRSO 2 -, or -NRSO 2 NR-;
- R is hydrogen or C1-C6 aliphatic;
- Z is an electron withdrawing substituent; and q is 0-3; and LG 1 is a first suitable leaving group
- first suitable conditions means conditions suitable to effectuate the reaction between compound of formula I-A and compound of formula R-I or between compound of formula II-A and compound of formula R-I.
- suitable conditions include, e.g., a first suitable solvent, a first suitable temperature, and a suitable reducing agent.
- suitable conditions include, e.g., a first suitable solvent, a first suitable temperature, and a suitable reducing agent.
- the first suitable solvent is a polar aprotic, a polar protic solvent, an apolar solvent, or a suitable combination thereof.
- Exemplary solvents useful as first suitable solvent include methanol, ethanol, propanol, isopropanol, t-butanol, dichloromethane, dichloroethane, toluene, tetrahydrofuran, dioxane, diethylether, dimethylether, acetonitrile, dimethylformamide, DMAC, or NMP.
- the first suitable temperature is a temperature that is sufficient to effectuate the reaction between compound of formula I-A and compound of formula R-I in the first suitable solvent. In another embodiment, the first suitable temperature is a temperature that is sufficient to effectuate the reaction between compound of formula II-A and compound of formula R-I in the first suitable solvent. Exemplary first suitable temperature includes between about 0 °C to about 110 °C. In one embodiment, the first suitable temperature is between about 0 0 C to about 25 0 C.
- the suitable reducing agent is a reducing agent that is capable effectuating the reaction between compound of formula I-A and compound of formula R-I.
- the suitable reducing agent is a reducing agent that is capable effectuating the reaction between compound of formula H-A and compound of formula R-I.
- Agents suitable for the present invention include a metallo-borohydride or a reagent capable of catalytic hydrogenation.
- Exemplary such suitable reducing agents include sodium borohydride, sodium cyanoborohydride, lithium borohydride, sodium triacetoxyborohydride, calcium borohydride, hydrogene in the presence of a suitable metal catalyst such as Pd/C.
- LG 1 is a first suitable leaving group that is capable of displacement to produce compound of formula I. or compound of formula II. See, "Advanced Organic Chemistry: Reactions, Mechanisms, and Structure,” pp. 339-357, Jerry March, 4 th Ed., John Wiley & Sons (1992).
- LG 1 is selected from alkysulfonate, arylsulfonate, halide, alkyl carboxylate.
- a "suitable chiral auxiliary" above in compound of formula H-B or formula I-B is a chiral auxiliary that is capable of attachment to an amino group.
- An isomeric mixture of a compound containing such an amino group with a suitable chiral auxiliary attached thereto is readily separated into its individual isomers by suitable separation means. See, for example, J. Jacques et al., "Enantiomers, Racemates And Resolutions," pp. 251-369, John Wiley & Sons, New York (1981); E. L. Eliel & S. H. Wilen, "Stereochemistry of Organic Compounds," pp. 868-870, John Wiley & Sons (1994).
- said suitable chiral auxiliary is an alkylsulfoxyl group.
- said suitable second conditions comprises a suitable protic acid and a suitable second solvent.
- said suitable second solvent is selected from a polar aprotic solvent or a protic solvent.
- polar aprotic solvents include dioxane, tetrahydrofurane, diethyl ethez * , dichloromethane, etc.
- protic solvents include methanol, ethanol, z-propanaol, ⁇ -butanol, etc.
- said suitable second solvent is a polar aprotic solvent.
- said compound of formula I-B and compound of formula H-B is produced by reacting, respectively, a compound of formula I-C or formula II- C with a compound of formula R-2 under a third suitable conditions:
- R is hydrogen or C1-C6 aliphatic
- R 1 is hydrogen or a first suitable protecting group
- [CA] is a suitable chiral auxiliary
- LG 2 is a second suitable leaving group.
- LG 2 is selected from halide, OC(O)(Cl-Co alkyl), pentafluorophenoxy, C1-C6 alkoxy, OCO 2 (C 1-C6 alkyl) or hydroxy.
- R 1 is hydrogen
- said third suitable conditions comprises a third suitable coupling agent and a third suitable solvent.
- said suitable coupling agent is selected from DCC 5 DCI, HATU, TCPH, or HBTU.
- said third suitable solvent is selected from dichloromethane, dioxane, acetonitrile, DMF, dichloroethane, or tetrahydrofuran.
- said compound of formula I-C or formula H-C is produced from an isomeric mixture of a compound of formula R-3:
- R, Z, and q are as defined above;
- PGj is a second suitable protecting group; and [CA] is a suitable chiral auxiliary; said method comprising two steps, wherein one of said two steps is separating said isomeric mixture using suitable separation means, and the other of said two steps is the conversion OfPG 1 to R 1 in a fourth suitable conditions.
- said suitable separation means comprises suitable chromatographic means.
- suitable chromatographic means include column chromatography or thin layer chromatography.
- said suitable separation means comprises suitable crystallization means.
- said fourth suitable conditions comprises a suitable deprotecting reagent and a fourth suitable solvent.
- An exemplary suitable deprotecting reagent is trifluoroacetic acid.
- said fourth suitable solvent is a polar aprotic solvent.
- exemplary solvents include dichloromethane, tetrahydrofuran, dioxane, diethyl ether, etc.
- said compound of formula R-3 is produced from a compound of formula R-4 and a compound of formula R-5:
- PG 1 is a second suitable protecting group; M is a suitable metal cation; and [CA] is a suitable chiral auxiliary; said method comprising the steps of reacting said compound of formula R-4 with said compound of formula R-5 in a fifth suitable conditions.
- said M is selected from Li + , Na + , or Mg +"1" .
- said PG 1 is selected from an alkylcarbamate, trifluoroacetyl, trialkylsilyl, or pivaloyl. Or, said PG 1 is BOC or trimethylsilyl.
- said fifth suitable conditions comprises a fifth suitable solvent and a fifth suitable temperature. In one embodiment, said suitable temperature is about -78 degrees C.
- said fifth suitable solvent is tetrahydrofuran.
- said compound of formula R-I is:
- said compound of formula I or formula II are selected from Table 1.
- Scheme I-B illustrates a process for producing intermediate B.
- Scheme I-B a) Br 2 ; b) thiourea, (Me) 2 NCH(OMe) 2 ; c) HCl
- Scheme H-A illustrates another exemplary process for preparing compounds of the present invention using a chiral auxiliary.
- Scheme II-B illustrates a process for preparing an exemplary compound of the present invention using a chiral auxiliary.
- Scheme III illustrates another process for preparing a compound of the present invention.
- the present invention provides compounds that are useful as modulators of CFTR and thus are useful in the treatment of disease, disorders or conditions such as Cystic fibrosis, Hereditary emphysema, Hereditary hemochromatosis, Coagulation- Fibrinolysis deficiencies, such as Protein C deficiency, Type 1 hereditary angioedema, Lipid processing deficiencies, such as Familial hypercholesterolemia, Type 1 chylomicronemia, Abetalipoproteinemia, Lysosomal storage diseases, such as I-cell disease/Pseudo-Hurler, Mucopolysaccharidoses, Sandhof/Tay-Sachs, Crigler-Najjar type II, Polyendocrinopathy/Hyperinsulemia, Diabetes mellitus, Laron dwarfism, Myleoperoxidase deficiency, Primary hypoparathyroidism, Melanoma, Glycanosis CDG type 1, Hereditary
- compositions comprising any of the compounds as described herein, and optionally comprise a pharmaceutically acceptable carrier, adjuvant or vehicle.
- these compositions optionally further comprise one or more additional therapeutic agents.
- a pharmaceutically acceptable derivative includes, but is not limited to, pharmaceutically acceptable salts, esters, salts of such esters, or any other adduct or derivative which upon administration to a patient in need is capable of providing, directly or indirectly, a compound as otherwise described herein, or a metabolite or residue thereof.
- the term “pharmaceutically acceptable salt” refers to those salts which are, within the scope of sound medical judgement, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio.
- a “pharmaceutically acceptable salt” means any non-toxic salt or salt of an ester of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof.
- the term “inhibitorily active metabolite or residue thereof means that a metabolite or residue thereof is also an inhibitor of an ATP -Binding Cassette Transporters.
- compositions of this invention include those derived from suitable inorganic and organic acids and bases.
- Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
- inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid
- organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
- salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, fo ⁇ nate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2- naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pec
- Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N + (C i-4alkyl)4 salts.
- This invention also envisions the quaternization of any basic nitrogen- containing groups of the compounds disclosed herein. Water or oil-soluble or dispersable products may be obtained by such quaternization.
- Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like.
- Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate and aryl sulfonate.
- the pharmaceutically acceptable compositions of the present invention additionally comprise a pharmaceutically acceptable carrier, adjuvant, or vehicle, which, as used herein, includes any and all solvents, diluents, or other liquid vehicle, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants and the like, as suited to the particular dosage form desired.
- a pharmaceutically acceptable carrier, adjuvant, or vehicle which, as used herein, includes any and all solvents, diluents, or other liquid vehicle, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants and the like, as suited to the particular dosage form desired.
- Remington's Pharmaceutical Sciences, Sixteenth Edition, E. W. Martin (Mack Publishing Co., Easton, Pa., 1980) discloses various carriers used in formulating pharmaceutically acceptable compositions
- any conventional carrier medium is incompatible with the compounds of the invention, such as by producing any undesirable biological effect or otherwise interacting in a deleterious manner with any other component(s) of the pharmaceutically acceptable composition, its use is contemplated to be within the scope of this invention.
- materials which can serve as pharmaceutically acceptable carriers include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, or potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, wool fat, sugars such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxyniethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; ge
- the present invention provides a method of treating a condition, disease, or disorder implicated by CFTR.
- the present invention provides a method of treating a condition, disease, or disorder implicated by a deficiency of CFTR, the method comprising administering a composition comprising a compound of formula (I) to a subject, preferably a mammal, in need thereof.
- the present invention provides a method of treating cystic fibrosis, hereditary emphysema (due to al -antitrypsin; non Piz variants), hereditary hemochromatosis, coagulation-fibrinolysis deficiencies, such as protein C deficiency, Type 1 hereditary angioedema, lipid processing deficiencies, such as familial hypercholesterolemia, Type 1 chylomicronemia, abetalipoproteinemia, lysosomal storage diseases, such as I-cell disease/pseudo-Hurler, mucopolysaccharidoses (due to lysosomal processing enzymes), Sandhof/Tay-Sachs (due to ⁇ -hexosaminidase), Crigler-Najjar type II (due to UDP-glucuronyl-sialyc-transferase), polyendocrinopathy/hyperinsulemia, diabetes mell
- the diseases associated with the latter class of ER malfunction are glycanosis CDG type 1, hereditary emphysema (due to ⁇ l -antitrypsin (PiZ variant), congenital hyperthyroidism, osteogenesis imperfecta (due to Type I, II, IV procollagen), hereditary hypofibrinogenemia (due to fibrinogen), ACT deficiency (due to ⁇ l- antichymotrypsin), diabetes insipidus (DI), neurophyseal DI (due to vasopvessin hormone/V2- receptor), neprogenic DI (due to aquaporin II), Charcot-Marie Tooth syndrome (due to peripheral myelin protein 22), Perlizaeus-Merzbacher disease, neurodegenerative diseases such as Alzheimer's disease ( due to ⁇ APP and presenilins), Parkinson's disease, amyotrophic lateral sclerosis, progressive supranuclear plasy, Pick'
- the present invention provides a method of treating cystic fibrosis comprising the step of administering to said mammal a composition comprising the step of administering to said mammal an effective amount of a composition comprising a compound of formula (I), or a preferred embodiment thereof as set forth above.
- an "effective amount" of the compound or pharmaceutically acceptable composition is that amount effective for treating or lessening the severity of one or more of cystic fibrosis, hereditary emphysema (due to al -antitrypsin; non Piz variants), hereditary hemochromatosis, coagulation-fibrinolysis deficiencies, such as protein C deficiency, Type 1 hereditary angioedema, lipid processing deficiencies, such as familial hypercholesterolemia, Type 1 chylomicronemia, abetalipoproteinemia, lysosomal storage diseases, such as I-cell disease/pseudo-Hurler, mucopolysaccharidoses (due to lysosomal processing enzymes), Sandhof/Tay-Sachs (due to ⁇ -hexosaminidase), Crigler-Najjar type II (due to UDP-glucuronyl-sialyc-transfer
- the diseases associated with the latter class of ER malfunction are glycanosis CDG type 1, hereditary emphysema (due to ⁇ l -antitrypsin (PiZ variant), congenital hyperthyroidism, osteogenesis imperfecta (due to Type I, II, IV procollagen), hereditary hypofibrinogenemia (due to fibrinogen), ACT deficiency (due to ⁇ l- antichymotrypsin), diabetes insipidus (DI), neurophyseal DI (due to vasopvessin hormone/V2- receptor), neprogenic DI (due to aquaporin II), Charcot-Marie Tooth syndrome (due to peripheral myelin protein 22), Perlizaeus-Merzbacher disease, neurodegenerative diseases such as Alzheimer's disease ( due to ⁇ APP and presenilins), Parkinson's disease, amyotrophic lateral sclerosis, progressive supranuclear plasy, Pick'
- the compounds and compositions, according to the method of the present invention may be administered using any amount and any route of administration effective for treating or lessening the severity of one or more of cystic fibrosis, hereditary emphysema (due to a 1 -antitrypsin; non Piz variants), hereditary hemochromatosis, coagulation-fibrinolysis deficiencies, such as protein C deficiency, Type 1 hereditary angioedema, lipid processing deficiencies, such as familial hypercholesterolemia, Type 1 chylomicronemia, abetalipoproteinemia, lysosomal storage diseases, such as I-cell disease/pseudo-Hurler, mucopolysaccharidoses (due to lysosomal processing enzymes), Sandhof/Tay-Sachs (due to ⁇ - hexosaminidase), Crigler-Najjar type II (due to UDP-glucuronyl
- the diseases associated with the latter class of ER malfunction are glycanosis CDG type 1, hereditary emphysema (due to ⁇ l -antitrypsin (PiZ variant), congenital hyperthyroidism, osteogenesis imperfecta (due to Type I, II, IV procollagen), hereditary hypofibrinogenemia (due to fibrinogen), ACT deficiency (due to ⁇ l-antichymotrypsin), diabetes insipidus (DI), neurophyseal DI (due to vasop ' vessin hormone/V2 -receptor), neprogenic DI (due to aquaporin II), Charcot-Marie Tooth syndrome (due to peripheral myelin protein 22), Perlizaeus- Merzbacher disease, neurodegenerative diseases such as Alzheimer's disease ( due to ⁇ APP and presenilins), Parkinson's disease, amyotrophic lateral sclerosis, progressive supranuclear
- the exact amount required will vary from subject to subject, depending on the species, age, and general condition of the subject, the severity of the infection, the particular agent, its mode of administration, and the like.
- the compounds of the invention are preferably formulated in dosage unit form for ease of administration and uniformity of dosage.
- dosage unit form refers to a physically discrete unit of agent appropriate for the patient to be treated. It will be understood, however, that the total daily usage of the compounds and compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment.
- the specific effective dose level for any particular patient or organism will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed, and like factors well known in the medical arts.
- patient means an animal, preferably a mammal, and most preferably a human.
- compositions of this invention can be administered to humans and other animals orally, rectally, parenterally, intracisternally, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops), bucally, as an oral or nasal spray, or the like, depending on the severity of the infection being treated.
- the compounds of the invention may be administered orally or parenterally at dosage levels of about 0.01 mg/kg to about 50 mg/kg and preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.
- Liquid dosage forms for oral administration include, but are not limited to, pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs.
- the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emu ⁇ sif ⁇ ers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
- the oral compositions can contain inert diluents commonly used in the art such as,
- Injectable preparations for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents.
- the sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol.
- acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution.
- sterile, fixed oils are conventionally employed as a solvent or suspending medium.
- injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.
- a compound of the present invention hi order to prolong the effect of a compound of the present invention, it is often desirable to slow the absorption of the compound from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the compound then depends upon its rate of dissolution that, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered compound form is accomplished by dissolving or suspending the compound in an oil vehicle. Injectable depot forms are made by fo ⁇ ning microencapsule matrices of the compound in biodegradable polymers such as polylactide-polyglycolide.
- the rate of compound release can be controlled.
- biodegradable polymers include poly(orthoesters) and poly(anhydrides).
- Depot injectable formulations are also prepared by entrapping the compound in liposomes or microemulsions that are compatible with body tissues.
- compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
- suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
- Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules.
- the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as. sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar—agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absoiption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example
- Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
- the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical fonnulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes.
- Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like.
- the active compounds can also be in microencapsulated form with one or more excipients as noted above.
- the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art. Pn such solid dosage forms the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch.
- Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose.
- the dosage forms may also comprise buffering agents. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner.
- buffering agents include polymeric substances and waxes.
- Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches.
- the active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required.
- Ophthalmic formulation, eardrops, and eye drops are also contemplated as being within the scope of this invention.
- the present invention contemplates the use of transdermal patches, which have the added advantage of providing controlled delivery of a compound to the body.
- Such dosage forms are prepared by dissolving or dispensing the compound in the proper medium.
- Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel.
- the compounds of the invention are useful as modulators of CFTR.
- the compounds and compositions are particularly useful for treating or lessening the severity of a disease, condition, or disorder where hyperactivity or inactivity of CFTR is implicated in the disease, condition, or disorder.
- the disease, condition, or disorder may also be referred to as a "CFTR-mediated disease, condition or disorder".
- the present invention provides a method for treating or lessening the severity of a disease, condition, or disorder where hyperactivity or inactivity of an CFTR is implicated in the disease state.
- the compounds and pharmaceutically acceptable compositions of the present invention can be employed in combination therapies, that is, the compounds and pharmaceutically acceptable compositions can be administered concurrently with, prior to, or subsequent to, one or more other desired therapeutics or medical procedures.
- the particular combination of therapies (therapeutics or procedures) to employ in a combination regimen will take into account compatibility .of the desired therapeutics and/or procedures and the desired therapeutic effect to be achieved.
- the therapies employed may achieve a desired effect for the same disorder (for example, an inventive compound may be administered concurrently with another agent used to treat the same disorder), or they may achieve different effects (e.g., control of any adverse effects).
- additional therapeutic agents that are normally administered to treat or prevent a particular disease, or condition are known as "appropriate for the disease, or condition, being treated".
- the amount of additional therapeutic agent present in the compositions of this invention will be no more than the amount that would normally be administered in a composition comprising that therapeutic agent as the only active agent.
- the amount of additional therapeutic agent in the presently disclosed compositions will range from about 50% to 100% of the amount normally present in a composition comprising that agent as the only therapeutically active agent.
- the present invention in another aspect, includes a composition for coating an implantable device comprising a compound of the present invention as described generally above, and in classes and subclasses herein, and a carrier suitable for coating said implantable device.
- the present invention includes an implantable device coated with a composition comprising a compound of the present invention as described generally above, and in classes and subclasses herein, and a carrier suitable for coating said implantable device.
- Suitable coatings and the general preparation of coated implantable devices are described in US Patents 6,099,562; 5,886,026; and 5,304,121.
- the coatings are typically biocompatible polymeric materials such as a hydrogel polymer, polymethyldisiloxane, polycaprolactone, polyethylene glycol, polylactic acid, ethylene vinyl acetate, and mixtures thereof.
- the coatings may optionally be further covered by a suitable topcoat of fluorosilicone, polysaccarides, polyethylene glycol, phospholipids or combinations thereof to impart controlled release characteristics in the composition.
- Another aspect of the invention relates to modulating CFTR activity in a biological sample or a patient (e.g., in vitro or in vivo), which method comprises administering to the patient, or contacting said biological sample with a compound of formula I or a composition comprising said compound.
- biological sample includes, without limitation, cell cultures or extracts thereof; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof.
- Modulation of CFTR activity in a biological sample is useful for a variety of purposes that are known to one of skill in the art. Examples of such memeposes include, but are not limited to, the study of CFTR in biological and pathological phenomena; and the comparative evaluation of new modulators of CFTR.
- a method of modulating activity of an anion channel in vitro or in vivo comprising the step of contacting said channel with a compound of formula (I).
- the anion channel is a chloride channel or a bicarbonate channel, hi other preferred embodiments, the anion channel is a chloride channel.
- the present invention provides a method of increasing the number of functional CFTR in a membrane of a cell, comprising the step of contacting said cell with a compound of formula (T).
- a compound of formula (T) as used herein means an CFTR that is capable of transport activity.
- the activity of the CFTR is measured by measuring the transmembrane voltage potential.
- Means for measuring the voltage potential across a membrane in the biological sample may employ any of the known methods in the art, such as optical membrane potential assay or other electrophysiological methods.
- the optical membrane potential assay utilizes voltage-sensitive FRET sensors described by Gonzalez and Tsien (Sea, Gonzalez, J. E. and R. Y. Tsien (1995) "Voltage sensing by fluorescence resonance energy transfer in single cells” Biophvs J 69(4): 1272-80, and Gonzalez, J. E. and R. Y. Tsien (1997) "Improved indicators of cell membrane potential that use fluorescence resonance energy transfer” Chem Biol 4(4): 269-77) in combination with instrumentation for measuring fluorescence changes such as the Voltage/Ion Probe Reader (VIPR) (Se ⁇ 1 Gonzalez, J. E., K. Oades, et al. (1999) "Cell-based assays and instrumentation for screening ion-channel targets” Drug Discov Today 4(9): 431-439).
- VIP Voltage/Ion Probe Reader
- These voltage sensitive assays are based on the change in fluorescence resonant energy transfer (FRET) between the membrane-soluble, voltage-sensitive dye, DiSBAC 2 (3), and a fluorescent phospholipid, CC2-DMPE, which is attached to the outer leaflet of the plasma membrane and acts as a FRET donor.
- FRET fluorescence resonant energy transfer
- Changes in membrane potential (V 1n ) cause the negatively charged DiSBAC 2 (3) to redistribute across the plasma membrane and the amount of energy transfer from CC2-DMPE changes accordingly.
- the changes in fluorescence emission can be monitored using VIPRTM II, which is an integrated liquid handler and fluorescent detector designed to conduct cell-based screens in 96- or 384-well microtiter plates.
- the present invention provides a kit for use in measuring the activity of a CFTR or a fragment thereof in a biological sample in vitro or in vivo comprising (i) a composition comprising a compound of formula (I) or any of the above embodiments; and (ii) instructions for a) contacting the composition with the biological sample and b) measuring activity of said CFTR or a fragment thereof.
- the kit further comprises instructions for a) contacting an additional composition with the biological sample; b) measuring the activity of said CFTR or a fragment thereof in the presence of said additional compound, and c) comparing the activity of the CFTR in the presence of the additional compound with the density of the CFTR in the presence of a composition of formula (I).
- the kit is used to measure the density of CFTR.
- the dark brown/black reaction mixture was diluted with water (400 mL) and extracted twice with equal volumes ethyl acetate and dichloromethane.
- the basic aqueous solution was acidified with concentrated hydrochloric acid to pH less than one and the precipitate was filtered and washed with 1 M hydrochloric acid.
- the solid material was dissolved in dichloromethane (400 mL) and extracted twice with equal volumes of 1 M hydrochloric acid and once with a saturated aqueous solution of sodium chloride.
- the organic solution was dried over sodium sulfate and evaporated to dryness to give a white to slightly off- white solid (5.23 g, 25.4 mmol, 80.1 %).
- ESI-MS m/z calc.
- the first eluting product under these conditions had a retention time of 8.2 min (Chiralpak AS-H column). This product had a retention time 14.5 min. on a Chiralpak OJ- H 4.6 mm x 250 mm column (25% of a 50/50 (v/v) mixture of ethanol and methanol in hexanes at 1.0 mL/min.)
- the second eluting product (( ⁇ )-l-((R)-(2-(l-(benzo[ ⁇ /][l,3]dioxol-6- yl)cyclopropanecarboxamido)thiazoI-5-yl)(2-chlorophenyI)methyI)pyrroIidin-3-yI (liS',2i?,5S)-2-isopropyl-5-methylcyclohexyl carbonate) had a retention time of 28.6 min (Chiralpak AD column). ESI-MS m/z calc. 679.3, found; 680.5 (M+l) + ; Retention time 3.86 minutes.
- reaction mixture was allowed to warm up to 25 0 C, stirred at 25 0 C for 18 h, quenched with NaHCO 3 and then filtered through a short plug of Celite using EtOAc. The organic layer was separated from the aqueous layer and dried over Na 2 SO 4 and concentrated. The crude product was purified by column chromatography (0-25% EtOAc/Hexane) to provide (i?)-N-(2-chlorobenzylidene)-l,l-dimethylethylsulfinamide as a yellow liquid (45.1 g, 75%).
- the crude product was purified by column chromatography (0- 80% EtOAc/Hexane) to provide a diastereomeric mixture of (i?)-N-(l-((2-t- butoxylcarbonylamino)thiazol-5-yl)- 1 -(2-chlorophenyl)-methyl)- 1 , 1 -dimethyl ethylsulf ⁇ namide as a yellow solid (15.1 g, 83%) that was used directly in the next step.
- the crude product was purified by column chromatography (0- 5% EtOH/EtOAc) to provide (i?)-N-(,S)-l-(2-aminothiazol-5-yl)-l-(2-chlorophenyl)methyl)-l,l- dimethylethylsulfmamide and (RyN-(R)- 1 -(2-aminothiazol-5-yl)- 1 -(2-chlorophenyl)methyl)- 1.1- dimethylethylsulfinamide.
- reaction mixture was stirred at 25 0 C for 18 h, diluted with CH 2 Cl 2 and washed with 1 N HCl (50 mL X 2), NaHCO 3 (50 mL X 1) and brine (50 mL X 1).
- the organic layer was separated from the aqueous layer, dried over Na 2 SO 4 and concentrated.
- the dark brown/black reaction mixture was diluted with water (400 mL) and extracted twice with equal volumes of ethyl acetate and dichloromethane.
- the basic aqueous solution was acidified with concentrated hydrochloric acid to pH less than one and the precipitate was filtered and washed with 1 M hydrochloric acid.
- the solid material was dissolved in dichloromethane (400 mL) and washed twice with equal volumes of 1 M hydrochloric acid and once with brine.
- the organic solution was dried over sodium sulfate and evaporated to dryness to give a white to slightly off-white solid (5.23 g, 80.1 %).
- Method A Methyl 2,2-difluorobenzo[ ⁇ f][l,3]dioxole-5-carboxylate from previous step (11.5 g) dissolved in anhydrous tetrahydrofuran (20 mL) was slowly added to a suspension of lithium aluminum hydride (4.10 g, 106 mmol) in anhydrous THF (100 mL) at 0 0 C. The mixture was then warmed to room temperature and stirred at for 1 h. The reaction mixture was cooled to 0 °C and treated with water (4.1 g), followed by sodium hydroxide (10% aqueous solution, 4.1 mL). The resulting slurry was filtered and washed with THF.
- l-(2,2-difluorobenzo[ ⁇ i][l,3]dioxol-5-yl)cyclopropanecarboxylic acid was made by the procedure used for l-(benzo[ ⁇ f][l,3]dioxol-5-yi)cyclopropanecarboxylic acid, starting from 2-(2,2- difluorobenzo[ ⁇ i][l,3]dioxol-5-yl)acetonitrile. Yield (86%) of a white solid.
- (R)-N-(3,4-Dichlorobenzylidene)-2-methylpropane-2-sulfinamide was made by the procedure used for (R)-N-(2-chlorobenzylidene)-2-methylpropane-2-sulfinamide, starting from 3,4-dichlorobenzaldehyde and (i?)-2-methylpropanesulfmamide. Yield (93%) of a yellow oil that crystallizes upon standing.
- the crude product was purified by column chromatography to provide a diasteriomeric mixture of (S)-tert-butyl 5-((2-chloiO-4-fluorophenyi)(l,l- dimethylethylsulfinamido)methyl) thiazol-2-ylcarbamate as an orange-yellow solid (95%) that was used without further purification.
- the cmde product was purified by column chromatography to provide a diasteriomeric mixture of (R)-tert-butyl 5-((4-chloro-2-fluorophenyl)(l,l- dimethylethylsulfinamido)methyl) thiazol-2-ylcarbamate (78%) that was used without further purification.
- (R)-ferf-Butyl 5-((3 ,4-dichlorophenyl)( 1 , 1 -dimethylethylsulfmamido) methyl)thiazol-2-ylcarbamate was made by the procedure used for (R)-fert-butyl 5-((2-chloro- 4-fluorophenyl)(l,l-dimethylethylsulfinamido)methyl)thiazol-2-ylcarbamate, starting from tert-butyl thiazol-2-ylcarbamate and (R)-N-(3,4-dichlorobenzylidene)-2-methylpropane-2- sulfinamide.
- the crude product was purified by column chromatography (excess tert-butyl thiazol-2-ylcarbamate is eluted at 70% EtOAc/hexane, the desired product follows at 0-15% MeOH/EtOAc) to provide a diasteriomeric mixture of (R)-te/t-butyl 5-((3,4- dichlorophenyl)( 1,1 -dimethyl ethylsulfinamido)methyl)thiazol-2-ylcarbamate as a yellow solid (92%) that was used without further purification.
- N-(5-((S)-(2-Chloro-4-fluorophenyl)((R)- 1,1- dimethylethylsulfinamido)methyl) thiazol-2-yl)-l-(2,2-difluorobenzo[d][l,3]dioxol-5- yl)cyclopropanecarboxamide was made by the procedure used for l-(benzo[d][l,3]dioxol-5- yl)-N-(5-((S)-(2-chloro-4-fluorophenyl) ((R)- 1 , 1 -dimethylethylsulfmamido)methyl)thiazol-2- yl)cyclopropanecarboxamide, starting from l-(2,2-difluorobenzo[d][l,3]dioxol-5- yl)cyclopropanecarboxylic acid and (R)-N
- N-(5 ⁇ ((R)-(2-Chloro-4-fluorophenyr ⁇ (S)-l,l- dimethylethylsulfinamido)methyl)thiazol-2-yl)- 1 -(2,2-difluorobenzo[ ⁇ /] [ 1 ,3]dioxol-5- yl)cyclopropanecarboxamide was made by the procedure used for l-(benzo[d][l,3]dioxol-5- yl)-N-(5-((S)-(2-chloro-4-fluorophenyl) ((R)-1 , 1 -dimethylethylsulfmamido)methyl)thiazol-2- yl) cyclopropanecarboxamide, starting from l-(2,2-difluorobenzo[(i][l,3]dioxol-5- yl)cyclopropanecarboxylic acid and (S)-(
- N-(5-((S)-((R)-3-(ter ⁇ butyldimethylsilyloxy)pyrrolidin- 1 -yl)(2-chloro ⁇ 4- fluorophenyl) methyl)thiazol-2-yl)- 1 -(2,2-difluorobenzo[ ⁇ (][ 1 ,3]dioxol-5- yl)cyclopropanecarboxamide was made by the procedure used for l-(benzo[ ⁇ f
- N-(5-((R)-((R)-3-(tert-butyldimethylsilyloxy)pyrrolidin- 1 -yl)(2-chloro-4- fluorophenyl) methyl)thiazol-2-yl)-l -(2,2-difluorobenzo[ ⁇ fj [ 1 ,3]dioxol-5- yl)cyclopropanecarboxamide was made by the procedure used for l-(benzo[d][l,3]dioxol-5- yl)-N-(5-((S)-((R)-3 -(te ⁇ butyldimethylsilyloxy)pyrrolidin- 1 -yl)(2-chloro-4- fluorophenyl)methyl)thiazol-2-yl) cyclopropanecarboxamide, starting from (R)-N-(5-(amino(2- chloro-4-fluorophenyl)
- N-(5-((S)-(2-chloro-4-fluorophenyl)((R)-3-hydroxypyrrolidin-l- yl)methyl)thiazol-2-yl)-l-(2,2-difluorobenzo[J][l,3]dioxol-5-yl)cyclopropanecarboxamide was made by the procedure used for l-(benzo[d][l,3]dioxol-5-yl) ⁇ N-(5-((S)-(2-chloro-4- fluorophenyl) ((R)-3-hydroxypyrrolidin- 1 -yl)methyl)thiazol-2-yl)cyclopropanecarboxamide, starting from N-(5-((S)-((R)-3-(tert-butyldimethylsilyloxy)pynOlidin-l-yl)(2-chloro-4- fluoiOphenyl)methyl)thiazol-2
- N-(5-((R)-(2-Chloro-4-fluorophenyl)((R)-3-hydroxypyrrolidin-l- yl)methyl)thiazol-2-yl)-l-(2,2-difluorobenzo[(i][l,3]dioxol-5-yl)cyclopropanecarboxamide was made by the procedure used for l-(benzo[ ⁇ i][l,3]dioxol-5-yl)-N-(5-((S)-(2-chloro-4- fluorophenyl) ((R)-3-hydroxypyrrolidin- 1 -yl)methyl)thiazol-2-yl)cyclo ⁇ ropanecarboxamide, stalling from N-(5-((R)-((R)-3 -(te7 ⁇ -butyldimethylsilyloxy)pyrrolidin- 1 -yl)(2-chloro-4- fluorophenyl)
- N-(5-((S)-(4-chloro-2-fluorophenyl)((R)-3-hydroxypyrrolidin- 1 - yl)methyl)thiazol-2-yl)-l-(2,2-difluorobenzo[(i][l,3]dioxol-5-yl)cyclopropanecarboxamide was made by the procedure used for l-(benzo[d][l,3]dioxol ⁇ 5-yl)-N-(5-((S) ⁇ (2-chloro-4- fluorophenyl) ((R)-3-hydroxypyrrolidin- 1 -yl)methyl)thiazol-2-yl)cyclopropanecarboxamide, starting from N-(5-((S)-((R)-3-(tert-butyldimethylsilyloxy)pyrrolidin-l-yl)(4-chloro-2- fluorophenyl)methyl)thia
- the crude product was adsorbed onto silica gel and purified by column chromatography (70 - 100 % EtOAc/ hexanes.
- the EtOAc contained 2% NH 4 OH and was stirred to maintain the mixture) to provide (R)-N-((R)-(2-amino-4-methylthiazol-5-yl)(2- chlorophenyl)methyl)-2-methylpropane-2-sulfinamide and (R)-N-((S)-(2-amino-4- methylthiazol-5-yl)(2-chlorophenyl)methyl)-2-methylpropane-2-sulfinamide and a mixture of both diastereomers (7.1 g, 47 %).
- [l,3]dioxol-5-yi)cyclopropanecarboxamide (Isomer A) was made by the procedure used for N-(5-(amino(2-chlorophenyl)methyl)-4-methylthiazol-2-yl)-l-(benzo[(f][l,3]dioxol-5- yl)cyclopropanecarboxamide (Isomer A) starting from l-(benzo[ ⁇ /][l,3]dioxol-5-yl)-N-(5- ((3 ,4-dichlorophenyl)((R)- 1 , 1 -dimethylethylsulf ⁇ namido)methyl)thiazol-2- yl)cyclopropanecarboxamide (Isomer A).
- the optical membrane potential assay utilized voltage-sensitive FRET sensors described by Gonzalez and Tsien (See, Gonzalez, J. E. and R. Y. Tsien (1995) "Voltage sensing by fluorescence resonance energy transfer in single cells” Biophvs J 69(4): 1272-80, and Gonzalez, J. E. and R. Y. Tsien (1997) “Improved indicators of cell membrane potential that use fluorescence resonance energy transfer” Chem Biol 4(4): 269-77) in combination with instrumentation for measuring fluorescence changes such as the Voltage/Ion Probe Reader (VIPR) (See, . Gonzalez, J. E., K. Oades, et al. (1999) "Cell-based assays and instrumentation for screening ion-channel targets” Drug Discov Today 4(9): 431-439).
- VIPR Voltage/Ion Probe Reader
- NIH3T3 mouse fibroblasts stably expressing ⁇ F508-CFTR are used for optical measurements of membrane potential.
- the cells are maintained at 37 0 C in 5% CO 2 and 90 % humidity in Dulbecco's modified Eagle's medium supplemented with 2 mM glutamine, 10 % fetal bovine serum, 1 X NEAA, ⁇ -ME, 1 X pen/strep, and 25 mM HEPES in 175 cm 2 culture flasks.
- the cells were seeded at 30,000/well in 384- well matrigel-coated plates and cultured for 2 hrs at 37 0 C before culturing at 27 0 C for 24 hrs.
- Ussing chamber experiments were performed on polarized epithelial cells expressing ⁇ F508-CFTR to further characterize the ⁇ F508-CFTR modulators identified in the optical assays.
- pR r p ⁇ F508 - CFTR epithelial cells grown on Costar Snapwell cell culture inserts were mounted in an Ussing chamber (Physiologic Instruments, Inc., San Diego, CA), and the monolayers were continuously short-circuited using a Voltage-clamp System (Department of Bioengineering, University of Iowa, IA, and, Physiologic Instruments, Inc., San Diego, CA). Transepithelial resistance was measured by applying a 2-mV pulse.
- the FRT epithelia demonstrated resistances of 4 K ⁇ / cm 2 or more.
- the solutions were maintained at 27 0 C and bubbled with air.
- the electrode offset potential and fluid resistance were corrected using a cell-free insert.
- the current reflects the flow of Cl " through ⁇ F508-CFTR expressed in the apical membrane.
- the Isc was digitally acquired using an MPlOOA-CE interface and AcqKnowledge software (V3.2.6; BIOPAC Systems, Santa Barbara, CA).
- Typical protocol utilized a basolateral to apical membrane Cl " concentration gradient.
- normal ringer was used on the basolateral membrane, whereas apical NaCl was replaced by equimolar sodium gluconate (titrated to pH 7.4 with NaOH) to give a large Cl " concentration gradient across the epithelium.
- AU experiments were performed with intact monolayers.
- forskolin (10 ⁇ M) and the PDE inhibitor, IBMX (100 ⁇ M) were applied followed by the addition of the CFTR potentiator, genistein (50 ⁇ M).
- the cells were incubated with 10 ⁇ M of the test compound for 24 hours at 37°C and the current density was compared to the 27°C and 37°C controls (% activity). Prior to recording, the cells were washed 3X with extracellular recording medium to remove any remaining test compound. Preincubation with 10 ⁇ M of correction compounds significantly increased the cAMP- and genistein-dependent current compared to the 37°C controls.
- Table 3 illustrates the EC50 and relative efficacy of exemplary embodiments of the present invention.
- Table 3 the following meanings apply:
- the compounds of the present invention exhibit unexpectedly better correction activity as measured by the assays above.
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AT06801307T ATE512145T1 (en) | 2005-08-11 | 2006-08-11 | CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR MODULATORS |
NZ566208A NZ566208A (en) | 2005-08-11 | 2006-08-11 | 2-Thiazolamide derivatives as modulators of cystic fibrosis transmembrane conductance regulators |
JP2008526246A JP5143738B2 (en) | 2005-08-11 | 2006-08-11 | Modulator of cystic fibrosis membrane conductance regulator |
EP06801307A EP1912983B1 (en) | 2005-08-11 | 2006-08-11 | Modulators of cystic fibrosis transmembrane conductance regulator |
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CA002618057A CA2618057A1 (en) | 2005-08-11 | 2006-08-11 | Modulators of cystic fibrosis transmembrane conductance regulator |
MX2008002019A MX2008002019A (en) | 2005-08-11 | 2006-08-11 | Modulators of cystic fibrosis transmembrane conductance regulator. |
IL189178A IL189178A0 (en) | 2005-08-11 | 2008-02-03 | Thiazolyl derivatives and pharmaceutical compositions containing the same |
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WO2014086687A1 (en) | 2012-12-03 | 2014-06-12 | Universita' Degli Studi Di Padova | A cftr corrector for the teatment of genetic disorders affecting striated muscle |
US9642831B2 (en) | 2014-10-31 | 2017-05-09 | Abbvie S.Á.R.L. | Substituted chromanes and method of use |
US9670163B2 (en) | 2005-12-28 | 2017-06-06 | Vertex Pharmaceuticals Incorporated | Solid forms of N-[2,4-bis(1,1-dimethylethyl)-5-hydroxyphenyl]-1,4-dihydro-4-oxoquinoline-3-carboxamide |
US9701639B2 (en) | 2014-10-07 | 2017-07-11 | Vertex Pharmaceuticals Incorporated | Co-crystals of modulators of cystic fibrosis transmembrane conductance regulator |
US9725440B2 (en) | 2007-05-09 | 2017-08-08 | Vertex Pharmaceuticals Incorporated | Modulators of CFTR |
US9732080B2 (en) | 2006-11-03 | 2017-08-15 | Vertex Pharmaceuticals Incorporated | Azaindole derivatives as CFTR modulators |
US9751890B2 (en) | 2008-02-28 | 2017-09-05 | Vertex Pharmaceuticals Incorporated | Heteroaryl derivatives as CFTR modulators |
US9751839B2 (en) | 2009-03-20 | 2017-09-05 | Vertex Pharmaceuticals Incorporated | Process for making modulators of cystic fibrosis transmembrane conductance regulator |
US9758510B2 (en) | 2006-04-07 | 2017-09-12 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-binding cassette transporters |
US9776968B2 (en) | 2007-12-07 | 2017-10-03 | Vertex Pharmaceuticals Incorporated | Processes for producing cycloalkylcarboxamido-pyridine benzoic acids |
WO2017173274A1 (en) * | 2016-03-31 | 2017-10-05 | Vertex Pharmaceuticals Incorporated | Modulators of cystic fibrosis transmembrane conductance regulator |
US9840499B2 (en) | 2007-12-07 | 2017-12-12 | Vertex Pharmaceuticals Incorporated | Solid forms of 3-(6-(1-(2,2-difluorobenzo[d][1,3]dioxol-5-yl)cyclopropanecarboxamido)-3-methylpyridin-2-yl) benzoic acid |
US9974781B2 (en) | 2006-04-07 | 2018-05-22 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-binding cassette transporters |
US10022352B2 (en) | 2006-04-07 | 2018-07-17 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-binding cassette transporters |
US10058546B2 (en) | 2012-07-16 | 2018-08-28 | Vertex Pharmaceuticals Incorporated | Pharmaceutical compositions of (R)-1-(2,2-difluorobenzo[D][1,3]dioxo1-5-y1)-N-(1-(2,3-dihydroxypropy1)-6-fluoro-2-(1-hydroxy-2-methylpropan-2-y1)-1H-indol-5-y1) cyclopropanecarbox-amide and administration thereof |
US10071979B2 (en) | 2010-04-22 | 2018-09-11 | Vertex Pharmaceuticals Incorporated | Process of producing cycloalkylcarboxamido-indole compounds |
US10076513B2 (en) | 2010-04-07 | 2018-09-18 | Vertex Pharmaceuticals Incorporated | Pharmaceutical compositions of 3-(6-(1-(2,2-difluorobenzo[D][1,3]dioxol-5-yl) cyclopropanecarboxamido)-3-methylpyridin-2-yl) benzoic acid and administration thereof |
US10081621B2 (en) | 2010-03-25 | 2018-09-25 | Vertex Pharmaceuticals Incorporated | Solid forms of (R)-1(2,2-difluorobenzo[D][1,3]dioxol-5-yl)-N-(1-(2,3-dihydroxypropyl)-6-fluoro-2-(1-hydroxy-2-methylpropan-2-yl)-1H-indol-5-yl)cyclopropanecarboxamide |
US10206877B2 (en) | 2014-04-15 | 2019-02-19 | Vertex Pharmaceuticals Incorporated | Pharmaceutical compositions for the treatment of cystic fibrosis transmembrane conductance regulator mediated diseases |
US10231932B2 (en) | 2013-11-12 | 2019-03-19 | Vertex Pharmaceuticals Incorporated | Process of preparing pharmaceutical compositions for the treatment of CFTR mediated diseases |
US10258624B2 (en) | 2014-10-06 | 2019-04-16 | Vertex Pharmaceuticals Incorporated | Modulators of cystic fibrosis transmembrane conductance regulator |
US10272046B2 (en) | 2012-02-27 | 2019-04-30 | Vertex Pharmaceuticals Incorporated | Pharmaceutical composition and administrations thereof |
US10302602B2 (en) | 2014-11-18 | 2019-05-28 | Vertex Pharmaceuticals Incorporated | Process of conducting high throughput testing high performance liquid chromatography |
US10570115B2 (en) | 2016-09-30 | 2020-02-25 | Vertex Pharmaceuticals Incorporated | Modulator of cystic fibrosis transmembrane conductance regulator, pharmaceutical compositions, methods of treatment, and process for making the modulator |
WO2020049189A1 (en) | 2018-09-09 | 2020-03-12 | Qanatpharma Gmbh | Use of cftr modulators for treating cerebrovascular conditions |
US10626111B2 (en) | 2004-01-30 | 2020-04-21 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-binding cassette transporters |
US10646481B2 (en) | 2008-08-13 | 2020-05-12 | Vertex Pharmaceuticals Incorporated | Pharmaceutical composition and administrations thereof |
US10654829B2 (en) | 2017-10-19 | 2020-05-19 | Vertex Pharmaceuticals Incorporated | Crystalline forms and compositions of CFTR modulators |
US10662192B2 (en) | 2004-06-24 | 2020-05-26 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-binding cassette transporters |
US10793547B2 (en) | 2016-12-09 | 2020-10-06 | Vertex Pharmaceuticals Incorporated | Modulator of the cystic fibrosis transmembrane conductance regulator, pharmaceutical compositions, methods of treatment, and process for making the modulator |
US11066417B2 (en) | 2018-02-15 | 2021-07-20 | Vertex Pharmaceuticals Incorporated | Modulators of cystic fibrosis transmembrane conductance regulator, pharmaceutical compositions, methods of treatment, and process for making the modulators |
US11179367B2 (en) | 2018-02-05 | 2021-11-23 | Vertex Pharmaceuticals Incorporated | Pharmaceutical compositions for treating cystic fibrosis |
US11253509B2 (en) | 2017-06-08 | 2022-02-22 | Vertex Pharmaceuticals Incorporated | Methods of treatment for cystic fibrosis |
US11414439B2 (en) | 2018-04-13 | 2022-08-16 | Vertex Pharmaceuticals Incorporated | Modulators of cystic fibrosis transmembrane conductance regulator, pharmaceutical compositions, methods of treatment, and process for making the modulator |
US11434201B2 (en) | 2017-08-02 | 2022-09-06 | Vertex Pharmaceuticals Incorporated | Processes for preparing pyrrolidine compounds |
US11465985B2 (en) | 2017-12-08 | 2022-10-11 | Vertex Pharmaceuticals Incorporated | Processes for making modulators of cystic fibrosis transmembrane conductance regulator |
US11517564B2 (en) | 2017-07-17 | 2022-12-06 | Vertex Pharmaceuticals Incorporated | Methods of treatment for cystic fibrosis |
US11584761B2 (en) | 2019-08-14 | 2023-02-21 | Vertex Pharmaceuticals Incorporated | Process of making CFTR modulators |
US11591350B2 (en) | 2019-08-14 | 2023-02-28 | Vertex Pharmaceuticals Incorporated | Modulators of cystic fibrosis transmembrane conductance regulator |
US11873300B2 (en) | 2019-08-14 | 2024-01-16 | Vertex Pharmaceuticals Incorporated | Crystalline forms of CFTR modulators |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ZA200602755B (en) | 2003-09-06 | 2007-06-27 | Vertex Pharma | Modulators of ATP-binding cassette transporters |
ATE437640T1 (en) * | 2003-11-14 | 2009-08-15 | Vertex Pharma | THIAZOLES AND OXAZOLES AS MODULATORS OF ATP BINDING CASSETTE TRANSPORTERS |
US7977322B2 (en) | 2004-08-20 | 2011-07-12 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-binding cassette transporters |
US8759335B2 (en) * | 2004-01-30 | 2014-06-24 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-binding cassette transporters |
EP1912983B1 (en) * | 2005-08-11 | 2011-06-08 | Vertex Pharmaceuticals, Inc. | Modulators of cystic fibrosis transmembrane conductance regulator |
AU2006336504C9 (en) * | 2005-12-28 | 2015-05-14 | Vertex Pharmaceuticals Incorporated | 1-(benzo [D] [1,3] dioxol-5-yl) -N- (phenyl) cyclopropane- carboxamide derivatives and related compounds as modulators of ATP-Binding Cassette transporters for the treatment of Cystic Fibrosis |
US7671221B2 (en) * | 2005-12-28 | 2010-03-02 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-Binding Cassette transporters |
US7754739B2 (en) * | 2007-05-09 | 2010-07-13 | Vertex Pharmaceuticals Incorporated | Modulators of CFTR |
WO2009038913A2 (en) | 2007-08-24 | 2009-03-26 | Vertex Pharmaceuticals Incorporated | Isothiazolopyridinones useful for the treatment of (inter alia) cystic fibrosis |
PT2217572E (en) | 2007-11-16 | 2014-02-17 | Vertex Pharma | Isoquinoline modulators of atp-binding cassette transporters |
US20100036130A1 (en) * | 2007-12-07 | 2010-02-11 | Vertex Pharmaceuticals Incorporated | Processes for producing cycloalkylcarboxamido-pyridine benzoic acids |
CN101998854A (en) * | 2007-12-07 | 2011-03-30 | 沃泰克斯药物股份有限公司 | Formulations of 3-(6-(1-(2,2-difluorobenzo[d][1,3]dioxol-5-yl) cycklopropanecarboxamido)-3-methylpyridin-2-yl)benzoic acid |
AU2009231993B2 (en) | 2008-03-31 | 2013-10-10 | Vertex Pharmaceuticals Incorporated | Pyridyl derivatives as CFTR modulators |
JP2012504143A (en) | 2008-09-29 | 2012-02-16 | バーテックス ファーマシューティカルズ インコーポレイテッド | 3- (6- (1- (2,2-difluorobenzo [d] [1,3] dioxol-5-yl) cyclopropanecarboxamido) -3-methylpyridin-2-yl) benzoic acid dosage unit |
ES2483690T3 (en) * | 2008-10-23 | 2014-08-07 | Vertex Pharmaceuticals Incorporated | Modulators of cystic fibrosis transmembrane conductance regulator |
BR112012026257A2 (en) | 2010-04-07 | 2017-03-14 | Vertex Pharma | solid forms of 3- (6- (1- (2-, 2-difluorbenzo [d] [1,3] dioxol-5-yl) cyclopropanecarboxamido) -3-methylpyridin-2-yl) benzoic acid |
US8563593B2 (en) | 2010-06-08 | 2013-10-22 | Vertex Pharmaceuticals Incorporated | Formulations of (R)-1-(2,2-difluorobenzo[D] [1,3] dioxol-5-yl)-N-(1-(2,3-dihydroxypropyl)-6-fluoro-2-(1-hydroxy-2-methylpropan-2-yl)-1H-indol-5-yl)cyclopropanecarboxamide |
TWI535712B (en) * | 2010-08-06 | 2016-06-01 | 阿斯特捷利康公司 | Chemical compounds |
IN2014KN00885A (en) | 2011-11-08 | 2015-10-02 | Vertex Pharma | |
US8674108B2 (en) | 2012-04-20 | 2014-03-18 | Vertex Pharmaceuticals Incorporated | Solid forms of N-[2,4-bis(1,1-dimethylethy)-5-hydroxyphenyl]-1,4-dihydro-4-oxoquinoline-3-carboxamide |
CN107746834A (en) * | 2013-03-27 | 2018-03-02 | 辉瑞爱尔兰制药公司 | For preparing the method and intermediate of Pregabalin |
KR101721029B1 (en) | 2014-10-16 | 2017-03-29 | 연세대학교 산학협력단 | Indolizino[3,2-c]quinoline derivatives, pharmaceutically acceptable salt thereof, preparation method thereof and pharmaceutical composition for treatment of cystic fibrosis containing the same as active ingredient |
AU2022265718A1 (en) | 2021-04-29 | 2023-11-02 | Novartis Ag | Deubiquitinase-targeting chimeras and related methods |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5304121A (en) | 1990-12-28 | 1994-04-19 | Boston Scientific Corporation | Drug delivery system making use of a hydrogel polymer coating |
US5886026A (en) | 1993-07-19 | 1999-03-23 | Angiotech Pharmaceuticals Inc. | Anti-angiogenic compositions and methods of use |
US6099562A (en) | 1996-06-13 | 2000-08-08 | Schneider (Usa) Inc. | Drug coating with topcoat |
Family Cites Families (87)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6426331B1 (en) * | 1998-07-08 | 2002-07-30 | Tularik Inc. | Inhibitors of STAT function |
PT1394150E (en) * | 1999-02-24 | 2011-02-17 | Hoffmann La Roche | 4-phenylpyridine derivatives and their use as nk-1 receptor antagonists |
TWI259180B (en) * | 2000-08-08 | 2006-08-01 | Hoffmann La Roche | 4-Phenyl-pyridine derivatives |
US20100074949A1 (en) * | 2008-08-13 | 2010-03-25 | William Rowe | Pharmaceutical composition and administration thereof |
JP2005520171A (en) * | 2001-04-10 | 2005-07-07 | トランス テック ファーマ,インコーポレイテッド | Probes, systems, and methods for drug discovery |
US7135575B2 (en) * | 2003-03-03 | 2006-11-14 | Array Biopharma, Inc. | P38 inhibitors and methods of use thereof |
WO2004080972A1 (en) | 2003-03-12 | 2004-09-23 | Vertex Pharmaceuticals Incorporated | Pirazole modulators of atp-binding cassette transporters |
DE602004022819D1 (en) * | 2003-06-06 | 2009-10-08 | Vertex Pharma | TRANSPORTER OF ATP-BINDING CASSETTE |
ZA200602755B (en) | 2003-09-06 | 2007-06-27 | Vertex Pharma | Modulators of ATP-binding cassette transporters |
US20050070718A1 (en) * | 2003-09-30 | 2005-03-31 | Abbott Gmbh & Co. Kg | Heteroaryl-substituted 1,3-dihydroindol-2-one derivatives and medicaments containing them |
EP1680411A2 (en) | 2003-10-08 | 2006-07-19 | Vertex Pharmaceuticals Incorporated | Modulators of atp-binding cassette transporters containing cycloalkyl or pyranyl groups |
ATE437640T1 (en) | 2003-11-14 | 2009-08-15 | Vertex Pharma | THIAZOLES AND OXAZOLES AS MODULATORS OF ATP BINDING CASSETTE TRANSPORTERS |
US7977322B2 (en) | 2004-08-20 | 2011-07-12 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-binding cassette transporters |
US8759335B2 (en) * | 2004-01-30 | 2014-06-24 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-binding cassette transporters |
US20140343098A1 (en) | 2004-06-24 | 2014-11-20 | Vertex Pharmaceuticals Incorporated | Modulators of atp-binding cassette transporters |
EP1773816B1 (en) * | 2004-06-24 | 2015-01-07 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-binding cassette transporters |
US8354427B2 (en) * | 2004-06-24 | 2013-01-15 | Vertex Pharmaceutical Incorporated | Modulators of ATP-binding cassette transporters |
US8242149B2 (en) * | 2005-03-11 | 2012-08-14 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-binding cassette transporters |
US8822451B2 (en) * | 2005-05-24 | 2014-09-02 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-binding cassette transporters |
EP1912983B1 (en) * | 2005-08-11 | 2011-06-08 | Vertex Pharmaceuticals, Inc. | Modulators of cystic fibrosis transmembrane conductance regulator |
RU2008118001A (en) | 2005-10-06 | 2009-11-20 | Вертекс Фармасьютикалз Инкорпорейтед (Us) | MODULATORS OF ATF-DEPENDENT TRANSPORTERS |
JP5317184B2 (en) | 2005-11-08 | 2013-10-16 | バーテックス ファーマシューティカルズ インコーポレイテッド | ATP binding cassette transporter modulator |
US20120232059A1 (en) | 2005-11-08 | 2012-09-13 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-Binding Cassette Transporters |
AU2006331614A1 (en) | 2005-12-24 | 2007-07-05 | Vertex Pharmaceuticals Incorporated | Quinolin- 4 - one derivatives as modulators of ABC transporters |
CA2635214A1 (en) * | 2005-12-27 | 2007-07-05 | Vertex Pharmaceuticals Incorporated | Compounds useful in cftr assays and methods therewith |
AU2006336504C9 (en) | 2005-12-28 | 2015-05-14 | Vertex Pharmaceuticals Incorporated | 1-(benzo [D] [1,3] dioxol-5-yl) -N- (phenyl) cyclopropane- carboxamide derivatives and related compounds as modulators of ATP-Binding Cassette transporters for the treatment of Cystic Fibrosis |
US7671221B2 (en) * | 2005-12-28 | 2010-03-02 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-Binding Cassette transporters |
LT1993360T (en) | 2005-12-28 | 2017-06-12 | Vertex Pharmaceuticals Incorporated | Solid forms of n-[2,4-bis(1,1-dimethylethyl)-5-hydroxyphenyl]-1,4-dihydro-4-oxoquinoline-3-carboxamide |
US7645789B2 (en) | 2006-04-07 | 2010-01-12 | Vertex Pharmaceuticals Incorporated | Indole derivatives as CFTR modulators |
CN101460489A (en) | 2006-04-07 | 2009-06-17 | 弗特克斯药品有限公司 | Modulators of ATP-binding cassette transporters |
US7553855B2 (en) * | 2006-05-12 | 2009-06-30 | Vertex Pharmaceuticals Incorporated | Compositions of N-[2,4-bis(1,1-dimethylethyl)-5-hydroxyphenyl]-1,4-dihydro-4-oxoquinoline-3-carboxamide |
US8563573B2 (en) * | 2007-11-02 | 2013-10-22 | Vertex Pharmaceuticals Incorporated | Azaindole derivatives as CFTR modulators |
US7754739B2 (en) * | 2007-05-09 | 2010-07-13 | Vertex Pharmaceuticals Incorporated | Modulators of CFTR |
EP2789606B1 (en) | 2007-05-09 | 2017-11-15 | Vertex Pharmaceuticals Incorporated | Modulators of CFTR |
WO2008147952A1 (en) * | 2007-05-25 | 2008-12-04 | Vertex Pharmaceuticals Incorporated | Modulators of cystic fibrosis transmembrane conductance regulator |
US20110177999A1 (en) | 2007-08-09 | 2011-07-21 | Vertex Pharmaceuticals Incorporated | Therapeutic Combinations Useful in Treating CFTR Related Diseases |
WO2009038913A2 (en) | 2007-08-24 | 2009-03-26 | Vertex Pharmaceuticals Incorporated | Isothiazolopyridinones useful for the treatment of (inter alia) cystic fibrosis |
EP2222304A2 (en) * | 2007-09-14 | 2010-09-01 | Vertex Pharmaceuticals Incorporated | Solid forms of n-[2,4-bis(1,1-dimethylethyl)-5-hydroxyphenyl]-1,4-dihydro-4-oxoquinoline-3-carboxamide |
NZ583878A (en) | 2007-09-14 | 2012-10-26 | Vertex Pharma | Modulators of ABC transporter and cystic fibrosis transmembrane conductance regulator (CFTR) |
PT2217572E (en) | 2007-11-16 | 2014-02-17 | Vertex Pharma | Isoquinoline modulators of atp-binding cassette transporters |
US20100036130A1 (en) * | 2007-12-07 | 2010-02-11 | Vertex Pharmaceuticals Incorporated | Processes for producing cycloalkylcarboxamido-pyridine benzoic acids |
LT2639223T (en) * | 2007-12-07 | 2017-06-26 | Vertex Pharmaceuticals Incorporated | Process for producing cycloalkylcarboxiamido-pyridine benzoic acids |
CN101998854A (en) * | 2007-12-07 | 2011-03-30 | 沃泰克斯药物股份有限公司 | Formulations of 3-(6-(1-(2,2-difluorobenzo[d][1,3]dioxol-5-yl) cycklopropanecarboxamido)-3-methylpyridin-2-yl)benzoic acid |
JP2011506330A (en) | 2007-12-07 | 2011-03-03 | バーテックス ファーマシューティカルズ インコーポレイテッド | Solid form of 3- (6- (1- (2,2-difluorobenzo [d] [1,3] dioxol-5-yl) cyclopropanecarboxamido) -3-methylpyridin-2-yl) benzoic acid |
WO2009076593A1 (en) * | 2007-12-13 | 2009-06-18 | Vertex Pharmaceuticals Incorporated | Modulators of cystic fibrosis transmembrane conductance regulator |
US8299099B2 (en) | 2008-02-28 | 2012-10-30 | Vertex Pharmaceuticals Incorporated | Heteroaryl derivatives as CFTR modulators |
AU2009231993B2 (en) | 2008-03-31 | 2013-10-10 | Vertex Pharmaceuticals Incorporated | Pyridyl derivatives as CFTR modulators |
BRPI0916877A2 (en) | 2008-08-13 | 2016-02-10 | Vertex Pharma | pharmaceutical composition and administration thereof |
US20100256184A1 (en) | 2008-08-13 | 2010-10-07 | Vertex Pharmaceuticals Incorporated | Pharmaceutical composition and administrations thereof |
JP2012504143A (en) | 2008-09-29 | 2012-02-16 | バーテックス ファーマシューティカルズ インコーポレイテッド | 3- (6- (1- (2,2-difluorobenzo [d] [1,3] dioxol-5-yl) cyclopropanecarboxamido) -3-methylpyridin-2-yl) benzoic acid dosage unit |
JP5645835B2 (en) | 2008-10-23 | 2014-12-24 | バーテックス ファーマシューティカルズ インコーポレイテッドVertex Pharmaceuticals Incorporated | N- (4- (7-azabicyclo [2.2.1] heptane-7-yl) -2- (trifluoromethyl) phenyl) -4-oxo-5- (trifluoromethyl) -1,4-dihydro Solid form of quinoline-3-carboxamide |
US20110257223A1 (en) | 2008-10-23 | 2011-10-20 | Vertex Pharmaceuticals Incorporated | Modulators of Cystic Fibrosis Transmembrane Conductance Regulator |
ES2483690T3 (en) * | 2008-10-23 | 2014-08-07 | Vertex Pharmaceuticals Incorporated | Modulators of cystic fibrosis transmembrane conductance regulator |
AU2009308232B2 (en) | 2008-10-23 | 2016-02-04 | Vertex Pharmaceuticals Incorporated | Modulators of cystic fibrosis transmembrane conductance regulator |
WO2010078103A1 (en) | 2008-12-30 | 2010-07-08 | Vertex Pharmaceuticals Incorporated | Modulators of cystic fibrosis transmembrane conductance regulator |
HUE038854T2 (en) | 2009-03-20 | 2018-12-28 | Vertex Pharma | Modulators of cystic fibrosis transmembrane conductance regulator |
WO2010108162A1 (en) * | 2009-03-20 | 2010-09-23 | Vertex Pharmaceuticals Incorporated | Process for making modulators of cystic fibrosis transmembrane conductance regulator |
JP5793496B2 (en) | 2009-09-17 | 2015-10-14 | バーテックス ファーマシューティカルズ インコーポレイテッドVertex Pharmaceuticals Incorporated | Process for preparing azabicyclic compounds |
EP2490687A1 (en) | 2009-10-22 | 2012-08-29 | Vertex Pharmaceuticals Incorporated | Compositions for treatment of cystic fibrosis and other chronic diseases |
CA2778493A1 (en) | 2009-10-23 | 2011-04-28 | Vertex Pharmaceuticals Incorporated | Solid forms of n-(4-(7-azabicyclo[2.2.1]heptan-7-yl)-2-trifluoromethyl)phenyl)-4-oxo-5-(trifluoromethyl)-1,4-dihydroquinoline-3-carboxamide |
KR20120104554A (en) | 2009-10-23 | 2012-09-21 | 버텍스 파마슈티칼스 인코포레이티드 | Process for preparing modulators of cystic fibrosis transmembrane conductance regulator |
EP2547658A1 (en) | 2010-03-19 | 2013-01-23 | Vertex Pharmaceuticals Incorporated | Solid forms of n-[2,4-bis(1,1-dimethylethyl)-5-hydroxyphenyl]-1,4-dihydro-4-oxoquinoline-3-carboxamide |
PL2826776T3 (en) | 2010-03-25 | 2021-05-31 | Vertex Pharmaceuticals Incorporated | Solid dispersion of amorphous form of (r)-1(2,2-difluorobenzo(d)(1,3)dioxol-5-yl)-n-(1-(2,3-dihydroxypropyl)-6-fluoro-2-(1-hydroxy-2-methylpropan-2-yl)-1h-indol-5-yl)-cyclopropanecarboxamide |
US8802868B2 (en) | 2010-03-25 | 2014-08-12 | Vertex Pharmaceuticals Incorporated | Solid forms of (R)-1(2,2-difluorobenzo[D][1,3]dioxo1-5-yl)-N-(1-(2,3-dihydroxypropyl-6-fluoro-2-(1-hydroxy-2-methylpropan2-yl)-1H-Indol-5-yl)-Cyclopropanecarboxamide |
BR112012026257A2 (en) | 2010-04-07 | 2017-03-14 | Vertex Pharma | solid forms of 3- (6- (1- (2-, 2-difluorbenzo [d] [1,3] dioxol-5-yl) cyclopropanecarboxamido) -3-methylpyridin-2-yl) benzoic acid |
BR112012026255A2 (en) | 2010-04-07 | 2017-03-14 | Vertex Pharma | 3- (6- (1- (2-, 2-Difluorbenzo [d] [1,3] dioxol-5-yl) cyclopropanecarboxamido) -3-methylpyridin-2-yl) benzoic acid pharmaceutical compositions and administration thereof |
NZ603042A (en) | 2010-04-22 | 2015-02-27 | Vertex Pharma | Pharmaceutical compositions comprising cftr modulators and administrations thereof |
CN105130948A (en) | 2010-04-22 | 2015-12-09 | 弗特克斯药品有限公司 | Pharmaceutical compositions and administrations thereof |
AU2011242457A1 (en) | 2010-04-22 | 2012-11-08 | Vertex Pharmaceuticals Incorporated | Pharmaceutical compositions and administrations thereof |
EP2560650A1 (en) | 2010-04-22 | 2013-02-27 | Vertex Pharmaceuticals Incorporated | Pharmaceutical compositions and administrations thereof |
US20110288122A1 (en) | 2010-05-20 | 2011-11-24 | Vertex Pharmaceuticals Incorporated | Pharmaceutical Compositions and Administrations Thereof |
US8404849B2 (en) | 2010-05-20 | 2013-03-26 | Vertex Pharmaceuticals | Processes for producing modulators of cystic fibrosis transmembrane conductance regulator |
US8563593B2 (en) | 2010-06-08 | 2013-10-22 | Vertex Pharmaceuticals Incorporated | Formulations of (R)-1-(2,2-difluorobenzo[D] [1,3] dioxol-5-yl)-N-(1-(2,3-dihydroxypropyl)-6-fluoro-2-(1-hydroxy-2-methylpropan-2-yl)-1H-indol-5-yl)cyclopropanecarboxamide |
WO2012027247A2 (en) | 2010-08-23 | 2012-03-01 | Vertex Pharmaceuticals Incorporated | Pharmaceutical composition of (r)-1-(2,2-difluorobenzo[d][1,3]dioxol-5-yl)-n-(1-(2,3-dihydroxy propyl)-6-fluoro-2-(1-hydroxy-2-methylpropan-2-yl)-1h-indol-5-yl) cyclopropanecarboxamide and administration therof |
CA2809263A1 (en) | 2010-08-27 | 2012-03-01 | Eleni Dokou | Pharmaceutical composition and administrations thereof |
US8802700B2 (en) | 2010-12-10 | 2014-08-12 | Vertex Pharmaceuticals Incorporated | Modulators of ATP-Binding Cassette transporters |
EP2773349A1 (en) | 2011-11-02 | 2014-09-10 | Vertex Pharmaceuticals Incorporated | Use of (n- [2, 4 -bis (1, 1 -dimethylethyl) - 5 - hydroxyphenyl]- 1, 4 - dihydro - 4 - oxoquinoline - 3 - carboxamide) for treating cftr mediated diseases |
IN2014KN00885A (en) | 2011-11-08 | 2015-10-02 | Vertex Pharma | |
ME03464B (en) | 2012-01-25 | 2020-01-20 | Vertex Pharma | Formulations of 3-(6-(1-(2.2-difluorobenzo[d][1,3]dioxol-5-yl) cyclopropanecarboxamido)-3-methylpyridin-2-yl)benzoic acid |
EP2819670A1 (en) | 2012-02-27 | 2015-01-07 | Vertex Pharmaceuticals Incorporated | Pharmaceutical composition and administration thereof |
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5304121A (en) | 1990-12-28 | 1994-04-19 | Boston Scientific Corporation | Drug delivery system making use of a hydrogel polymer coating |
US5886026A (en) | 1993-07-19 | 1999-03-23 | Angiotech Pharmaceuticals Inc. | Anti-angiogenic compositions and methods of use |
US6099562A (en) | 1996-06-13 | 2000-08-08 | Schneider (Usa) Inc. | Drug coating with topcoat |
Non-Patent Citations (27)
Title |
---|
"Handbook of Chemistry and Physics" |
"March's Advanced Organic Chemistry", 2001, JOHN WILEY & SONS |
ARIDOR M ET AL., NATURE MED., vol. 5, no. 7, 1999, pages 745 - 751 |
BROSS P. ET AL., HUMAN MUT., vol. 14, 1999, pages 186 - 198 |
CUTTING, G. R. ET AL., NATURE, vol. 346, 1990, pages 366 - 369 |
DALEMANS ET AL., NATURE LOND., vol. 354, 1991, pages 526 - 528 |
DEAN, M. ET AL., CELL, vol. 61, 1990 |
E. L. ELIEL, S. H. WILEN: "Stereochemistry of Organic Compounds", 1994, JOHN WILEY & SONS, pages: 868 - 870 |
E. W. MARTIN: "Remington's Pharmaceutical Sciences", 1980, MACK PUBLISHING CO. |
GONZALEZ, J. E., K. OADES ET AL.: "Cell-based assays and instrumentation for screening ion-channel targets", DRUG DISCOV TODAY, vol. 4, no. 9, 1999, pages 431 - 439 |
GONZALEZ, J. E., R. Y. TSIEN: "Improved indicators of cell membrane potential that use fluorescence resonance energy transfer", CHEM BIOL, vol. 4, no. 4, 1997, pages 269 - 77 |
GONZALEZ, J. E., R. Y. TSIEN: "Voltage sensing by fluorescence resonance energy transfer in single cells", BIOPHYS J, vol. 69, no. 4, 1995, pages 1272 - 80 |
GREGORY, R. J. ET AL., NATURE, vol. 347, 1990, pages 382 - 386 |
J. JACQUES ET AL.: "Enantiomers, Racemates And Resolutions", 1981, JOHN WILEY & SONS, pages: 251 - 369 |
J. PHARMACEUTICAL SCIENCES, vol. 66, 1977, pages 1 - 19 |
JERRY MARCH: "Advanced Organic Chemistry: Reactions, Mechanisms, and Structure", 1992, JOHN WILEY & SONS, pages: 14 - 16,18-19 |
JERRY MARCH: "Advanced Organic Chemistry: Reactions, Mechanisms, and Structure", 1992, JOHN WILEY & SONS, pages: 339 - 357 |
KEREM, B-S ET AL., PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 8447 - 8451 |
KEREM, B-S. ET AL., SCIENCE, vol. 245, 1989, pages 1073 - 1080 |
MORELLO, JP ET AL., TIPS, vol. 21, 2000, pages 466 - 469 |
PASYK, FOSKETT, J. CELL. BIOCHEM., vol. 270, 1995, pages 12347 - 50 |
QUINTON, P. M., FASEB J., vol. 4, 1990, pages 2709 - 2727 |
RICH, D. P. ET AL., NATURE, vol. 347, 1990, pages 358 - 362 |
RIORDAN, J. R. ET AL., SCIENCE, vol. 245, 1989, pages 1066 - 1073 |
RUTISHAUSER, J. ET AL., SWISS MED WKLY, vol. 132, 2002, pages 211 - 222 |
SHASTRY, B.S. ET AL., NEUROCHEM. INTERNATIONAL, vol. 43, 2003, pages 1 - 7 |
THOMAS SORRELL: "Organic Chemistry", 1999, UNIVERSITY SCIENCE BOOKS |
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AU2006279810B2 (en) | 2011-10-27 |
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CA2618057A1 (en) | 2007-02-22 |
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MX2008002019A (en) | 2008-04-16 |
US20150126566A1 (en) | 2015-05-07 |
JP5850983B2 (en) | 2016-02-03 |
AU2006279810A1 (en) | 2007-02-22 |
RU2008109031A (en) | 2009-09-20 |
NZ566208A (en) | 2010-09-30 |
US8962856B2 (en) | 2015-02-24 |
WO2007021982A3 (en) | 2007-05-03 |
US8586615B2 (en) | 2013-11-19 |
US20120010257A1 (en) | 2012-01-12 |
US7999113B2 (en) | 2011-08-16 |
US20080161371A1 (en) | 2008-07-03 |
US9351962B2 (en) | 2016-05-31 |
JP2015127347A (en) | 2015-07-09 |
JP2013018795A (en) | 2013-01-31 |
US9856248B2 (en) | 2018-01-02 |
EP1912983B1 (en) | 2011-06-08 |
NO20081280L (en) | 2008-05-09 |
US20170174675A9 (en) | 2017-06-22 |
US20160237079A1 (en) | 2016-08-18 |
JP5143738B2 (en) | 2013-02-13 |
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