NZ271634A

NZ271634A - 1-phenyl-4-(substituted alkyl)piperazine derivatives; pharmaceutical compositions

Info

Publication number: NZ271634A
Application number: NZ271634A
Authority: NZ
Inventors: Amedeo Leonardi; Gianni Motta; Carlo Riva; Rodolfo Testa
Original assignee: Recordati Chem Pharm
Priority date: 1993-07-30
Filing date: 1994-07-22
Publication date: 1996-09-25
Also published as: ITMI931717A0; ZA945625B; AU7532394A; JPH09500883A; CA2168443A1; KR960703884A; SG46281A1; WO1995004049A1; EP0711288A1; NO960371D0; AU680037B2; MXPA94005805A; NO960371L; CN1132508A; IT1266582B1; ITMI931717A1; IL110348A0

Description

<div class="application article clearfix" id="description"> New Zealand Paient Spedficaiion for Paient Number £71 634 « New Zealand No. 271634 International No. PCT/EP94/02437 Priority Date{s): Compile Specification Filod: Class: ^)..Cfiil3?.3,aa)..Q.5+Aft; Publication Date: .2..5..£EP..t996 P.O. Journal No: .1^9.$ NEW ZEALAND PATENTS ACT 1953 COMPLETE SPECIFICATION Title of Invention: Piperazine derivatives as alpha 1 A-adrenergic receptor antagonists Name, address and nationality of applicant(s) as in international application form: * co-po'.j RECORDATI S.A., CHEMICAL AND PHARMACEUTICAL COMPANY^of Corso S Gottardo 54, CH-6830 Chiasso, Switzerland 271634 -1- FIELD OF THE INVENTION The invention relates to piperazine derivatives and to pharmaceutical compositions containing them. BACKGROUND OF THE INVENTION Many different derivatives of piperazine are reported in the literature. For example, compounds of the general formula wherein R = one or more of H, halogen, alkyl and alkoxy, Y = CO, and Rl = H, halogen, methyl or methoxy, have been reported in US 2997472 as being active on the central nervous system. Other compounds of the same general formula, R^ and Y having the same meanings but R being a cyclic amino group, are disclosed in ZA 67/05794, and are again said to be active on the central nervous system. Further examples are given in Japanese Kokai 75-108264 where derivatives of this formula (R = H, alkoxy, halogen, Y = NH and Ri = 2-methoxy) are reported to have antiinflammatory, antipyretic, analgesic and blood pressure depressing activity. The compounds of the invention, described below, essentia^ y include bulky substituents on the phenyl ring linked to Y and/or new values for Y, not yet described in the literature for this class of derivatives. The combination.of the above and other structural elements, such as the nature of the piperazine substituent A, gives the new compounds the ability to interact with the a^-adrenergic receptor, and hence the possibility of selectively relaxing R Y-(CH2)n-N Rl 271634 -2- the tissues where these receptors are present. SUMMARY OF THE INVENTION The invention provides compounds of the general foirmula I: A wherein Y represents a valence bond or one of the following groups, each of which is depicted with its left hand end being the end which attaches to the phenyl group and its right hand end being the end which attaches to the group W: -S(0)m-, -N(R2)-, -N(R^)CO-, -PO(OC2H5)NH-, -CO-, -S02N(R2)—, -(CH2)nCOO- and -(CH2)nCON(R2)-, wherein m is l or 2, n is 0 to 2, and R2 represents a hydrogen atom, an alkyl group having up to 4 carbon atoms, a carbamoyl group, or an alkanoyl or alkylcarbamoyl group each having from 2 to 5 carbon atoms; w represents a linear or branched alkylene group having from 2 to 6 carbon atoms; A represents an alkoxy, alkyl or hydroxy group; A' represents one or more hydrogen or halogen atoms or alkoxy, alkyl or hydroxy groups; R represents a group of the formula -X-(CH2)p-Z, wherein X represents a valence bond or one of the following groups, each of which is depicted with its left hand end being the end which attaches to the phenyl group and its right hand end being the end which attaches to the group -(CH2)p-Z: N.Z. PATENT OFFICE 1 5 FEB 1996 RECEIVED 271634 -3- N.Z. PATENT OFFICE 1 5 FEB 1996 RECEIVED -o-, -s(0)n-, -co-, -n(R2)co- and -n(r2)so2-; wherein n and R2 are as above defined; p is 0 to 10, and Z represents a hydrogen atom, a cycloalkyl group having from 4 to 8 carbon atoms, a 1-naphthyl group, a 2-naphthyl group, a diphenylmethyl group or one of the groups having the following formulae CH=CH- wherein R3 represents one or more hydrogen or halogen atoms or cyano, hydroxy, alkoxy, alkyl, trifluoromethyl, alkanoyl, a-hydroxyalkyl, nitro, amino, alkylamino, dialkylamino, alkanoylamino, alkylsulphonylamino, phenyl, phenoxy or benzoyl groups; Rl represents a hydrogen or halogen atom or a cyano, hydroxy, alkyl, trifluoromethyl, alkanoyl, a-hydroxyalkyl, phenyl, phenoxy or benzoyl group; with the provisos that: when Y represents a valence bond or one of the groups -S(0)m-, -N(R2)-, -S02N(R2)-, -CH2CON(R2)- or -CH2CH2CON(R2)-, Z does not represent a hydrogen atom unless p is greater than 3; and when Y represents one of the groups -N(R2)CO-, -CO-, -(CH2)nCOO- or -CON(R2)-, the group R, not being a hydrogen atom, is in the ortho position relative to the moiety Y and either (i) the group R^, not being a hydrogen atom, is in the ortho position relative to the group R or (ii) Z does not represent a hydrogen atom unless p is greater than 3. The invention further includes prodrugs of the formula I compounds, e.g., the derivatives of compounds of formula I bearing reactive groups such as NH, NH2 and in particular OH 27163A N.Z. PATENT OFFu 1 5 FEB 1998 —4 — RECEIVED (i.e., at positions R^ or R3) prepared for various purposes, e.g., to improve the pharmacokinetic properties (adsorption, distribution, metabolism, plasmatic half-life, etc.) of said compounds of formula I, which can be administered in this "masked" or prodrug form and are liberated, exerting their pharmacological action, in mammals receiving them. Examples of these prodrug derivatives have the following structure: (Compound of formula I)-0C(0,S)-J-F wherein J represents a valence bond, an oxygen or sulphur atom or an amino group, F represents an alkyl group (optionally containing hetero atoms such as 0, S, N or substituted nitrogen) , a carbocyclic group or heterocyclic group, optionally substituted with amino, alkylamino, dialkylamino, dialkylaminoalkyl, carboxy, alkoxycarbonyl, carboxamido. Preferably, J represents a valence bond and F represents a methyl, t-butyl, n-butyl, B-CH2-phenyl, B-alkyl, B-CO-alkyl, HOCO-alkyl, alkyl-OCO-alkyl, where B represents a dialkylamino group or a cyclic amino group, optionally containing other heteroatoms such as N, 0 or S. Also included in the invention are derivatives having the formula (Compound I)-OP(O)(0Alkyl)2. The invention also includes the enantiomers, diastereoisomers, crystalline forms, solvates, N-oxides and pharmaceutically acceptable salts of these compounds, as well as metabolites of these compounds having the same type of activity (hereafter sometimes referred to as "active metabolites") and prodrugs of said "active metabolites". The invention further provides pharmaceutical compositions comprising a compound of Formula I or a prodrug, a metabolite, an enantiomer, diastereoisomer, crystalline form, solvate, N-oxide or pharmaceutically acceptable salt of such a compound or prodrug, in admixture with a pharmaceutically acceptable diluent or carrier. -5- DETAILED DESCRIPTION OF THE INVENTION 271634 N.Z. PATENT OFFICE 1 5 FEB 1996 RECEIVED The adrenergic antagonistic activity of compounds of the invention renders them useful as agents acting on body tissues particularly rich in aiA-adrenergic receptors (such as blood vessels, prostate, urethra, etc., as reported £y A. L. Morrow et al., Mol. Pharmacol., 29_, 321, 1986 and references cited therein in general and by H. Suzuki et al., Jap. J. Pharmacol., 58, suppl. 1, 173P, 1992 for the human prostate). As the subclassification of the a^-receptor subtypes is still in progress and is subject to review and refinement (see e.g. D. A. Schwinn et al., Eur. J. Pharmacol.-Mol. Pharmacol. Sect., 221. 433, 1992), it is intended that the adrenoceptor to which we refer is that one "pharmacologically11 characterized (see A.- L. Morrow supra), which is not alkylated by the selective alkylating agent chloroethylclonidine (CEC) , as described in the following experimental pharmacological section. Accordingly, antiadrenergic compounds within the invention established as such on the basis of their receptor binding profile, can be useful therapeutic agents for the treatment, for example, of micturition problems associated with obstructive disorders of the lower urinary "tract, including but not limited to benign prostatic hypertrophy (BPH), and of hypertension. Surprisingly, the compounds of the invention show high selectivity for the mammalian lower urinary tract, i.e., they are substantially more active in antagonizing urethral contractions than in lowering blood pressure. On the contrary, known a^-antagonists, such as prazosin which is 1-(4-amino-6,7-dimethoxy-2-quinazolinyl)-4-(2-furoyl)-pipera zine (GB 1156973), do not exhibit such selectivity (and in fact cause . hypotension as a most common side-effect). Urapidil, which is 6-{3-[4-(2-methoxyphenyl)-l-piperazinyl]-propylamino}-l,3--dimethyl-2,4-(lH,3H)-pyrimidinedioiie, a known 271634 N.2. PATtNT OFFICE 1 5 FEB 1996 -6- RECEIVED antihypertensive drug structurally closer than prazosin to the compounds of this invention, shows a very small selectivity and low affinity for the a ^-adrenoceptor. (Naturally, those compounds of the invention that are not selective for the lower urinary tract are preferred as antihypertensive agents, but even the selective compounds can often be used as antihypertensives because of their low toxicity.) The majority of the compounds of the invention exhibit low toxicity. Thus they can be used in higher amounts, an advantage that often more than compensates for a relatively lower level of activity that some of these compounds have. Naturally, those exhibiting both high activity and low toxicity are preferred. SYNTHESIS OF THE COMPOUNDS OF THE INVENTION In this section, the groups will be abbreviated as RRjPh and PzA respectively. L represents a halogen atom or a leaving group such as a tosyloxy group. M represents a group (CH) wherein n is 0 to 2. Q represents M-CO, SO2 or PO(OC2H5); Q' represents CO or SO2. X' represents O, s, N(alkyl) or NH. Hal represents a halogen atom. The compounds according to the invention may generally be prepared by condensation of compounds RR^Ph-Y-W-L with compounds H-PzA. The condensation is preferably, but not necessarily, carried out at a temperature of from 20 to 140°C in a polar solvent such as dimethylformamide (DMF) or methanol or in absence of any solvent at 100-200°C, usually in the presence of a base such as potassium carbonate, see Gibson's chapter in Patai, The Chemistry of the Amino Group, A and 271634 p.45 et seq., Wiley Interscience, New -7- alkylation is described in Example 23 below. Alternatively, under the same conditions, a compound RRlPh-Y-H can be condensed with a compound L-W-PzA. This condensation is illustrated in Examples 1 to 3 below.'When Y=NH, this condensation is preferably carried out on RRlPh-NH-Pg derivatives, where Pg is a protecting group which can be easily inserted and cleaved after the condensation has been performed. For example, this may be done by first reacting the amine RR1Ph~NH2 with an excess of trifluoroacetic anhydride, then reacting the obtained tri fluoroacetyl derivative RR1PI1-NH-COCF3 with an L-W-PzA reagent and finally deprotecting the resultant trifluoroacetyl derivative of the desired compound of the invention by treatment with potassium carbonate in aqueous methanol or with sodium borohydride in methanol or dimethylsulphoxide (DMSO). Further details on the use of this technique are reported by T.W. Greene in Protective Groups in Organic Synthesis, page 353, Wiley Interscience, 1991 and references cited therein. Another condensation method forms the group R by condensing a compound Z-(CH2)p-L with a compound A This condensation can be carried out as above described or by operating in protic or aprotic solvents (e.g. ethanol, acetonitrile, DMF, toluene) in the presence of a base such as potassium carbonate, sodium or sodium hydride at 20-140°C. This condensation is illustrated in Examples 6 to 18, 28, 30, .31 and 34 below. HX' Rl Some compounds of the invention can be prepared by addition reactions. For example, addition across a double bond is -8- 27165 N.Z. PATENT OFRCE 1 5 FEB 1996 RECEIVED possible, as in: RR1Ph-Y-CH=CH2 + H-PzA — RR1Ph-Y-CH2CH2-PzA (Y=CO or N(R2)CO; R2=H, C1-C4 alkyl), the reaction being carried out in aprotic solvents (e.g., toluene, xylene, DMSO, DMF) at from 25 to 150°C. Other synthetic schemes include the formation of Y or W during the reaction. Examples are: RRlPh-Q-Cl + R2-NH-W-PzA — RR1Ph-Q-NR2-W-PzA (R2=H, C1-C4 alkyl). The amidification is carried out in aprotic solvents e.g. haloalkanes, toluene, DMF or pyridine, optionally in the presence of an organic base such as triethylamine, or in aqueous dioxane or lower alkanols in the presence of inorganic bases such as sodium hydroxide, sodium bicarbonate or potassium carbonate, according to Beckwith in Zabicky, The Chemistry of Amides, page 731-857, Wiley, 1970. Such a reaction is illustrated in Examples 5, 33 and 35 below. RRjPh-M-COOH + R2-NH-W-PzA — RRiPh-M-CONH-W-PzA This reaction is carried out in the presence of a coupling agent (e.g. dicyclohexylcarbodiimide, N,N'-carbonyldiimidazole or diethyl cyanophosphonate) optionally in the presence of a promoting agent (e.g. 4-dimethylaminopyridine or N-hydroxybenzotriazole or N-hydroxysuccinimide) in an aprotic or a chlorinated solvent (e.g. DMF, chloroform) at from -10 to 140°C (Albertson, Org. React., 1_2, 205-218, 1962; Doherty et al., J. Med. Chem., 35, 9, 1992; Staab et al., Newer Methods Prep. Org. Chem., 5, 61, 1968; Ishihara, Chem. Pharm. Bull., 39, 3236, 1991). It is illustrated in Examples 4, 20 to 22, 24 to 27, 29, 32, 36 and 37 below. RRlPh-M-COOH + alkylchloroformate in the presence of a tertiary amine (e.g. triethylamine) followed by addition of R2-NH-W-PzA at from 0 to 80°C; optionally a promoting agent (e.g. 1-hydroxypyridine) may be added before the amine addition (Albertson, Org. React., 12, 157, 1962). RR]Ph-M-COOH + R2-NH-W-PzA — RRxPh-M-CONH-W-PzA This reaction can be carried out without a solvent at from —g- RECEIVED 150 to 220°C (Mitchell et al., J. Ara. Chem. SdV., S3, 1879, 1931) or in high-boiling ethereal solvents (e.g. diglyme). RRiPh-M-COO-Alk + R2-NH-W-PzA RRlPh-M-CONH-W-PzA RR^Ph-N(R2)-H + Cl-Q'-W-PzA — RRxPh-N(R2)-Q'-W-PzA RR^Ph—N(R2)-H + HOOC-W-PzA - RR^Ph-N(R2)-CO-W-PzA (R2=H or C1-C4 alkyl). These reactions may be carried out in an aprotic solvent such as hexane or in a chlorinated solvent such as dichloromethane at from -10 to 80°C, or without solvents at from 80 to 180°C. A coupling agent such as trimethylaluminium may be present. See S.M. Weinreb et al., Tetrahedron, 4171, 1977; and M.F. Lipton et al. , Org. Synth., 59, 49, 1979. RRiPh-M-CO-Hal + HO-W-PzA — RRqPIi-M-COO-W-PzA A simple esterification, as shown in Example 19. RR!Ph-NH2 + OHC-W-PZA — RR;|Ph-NH-W-PzA This reductive amination can be carried out in a lower alkanol as solvent at from 20 to 120°C. Sodium cyanoborohydride is suitable as the reducing agent. RRiPh-Y-Alk-CHO + H-PzA - RRiPh-Y-W-PzA In the conditions described for the previous reaction. This method is not suitable when Y=CO, COO or CH2COO. RRlPh-COCH3 + CH20 + H-PzA — RR1PhCOCH2CH2PzA This reaction can be carried out using formaldehyde or a polymer thereof in the presence of a mineral acid in alcohols, acetic acid or acetic anhydride at reflux, as reported by Tramontini et al. in Tetrahedron, £6, 1791, 1990 and references cited therein. Some of the compounds of the invention may be prepared by conversion of other compounds of the invention. For example: - Compounds I in which R3 represents an amino group may be prepared by reduction of the corresponding compounds in which R3 represents a nitro group. The reduction can be effected: (a) with Ni-Raney catalyst in a protic solvent selected from methanol, ethanol, isopropanol, water and mixtures of them; or (b) with SnCl2, H20, optionally in presence of 1 5 FEB 1936 ■VI .yf-rIGc -10- RECEIVED hydrochloric acid, either in a protic solvent such as methanol, ethanol, isopropanol, water, acetic acid or a mixture thereof, or in an aprotic solvent such as ethyl acetate; or (c) with Fe and aqueous hydrochloric acid in a protic solvent such as methanol, ethanol, isopropanol, water or a mixture thereof. The temperatures of the above reactions will be from -20°C to 100°C (J. March, Advanced Organic Chemistry, III Ed., page 1103, Wiley Interscience, 1985). - Compounds I in which R3 represents an amino group may be treated with an alkylating agent L-alkyl to give the corresponding compounds in which R3 represents an alkylamino or dialkylamino group. For monoalkylation, the amino group may be protected before the alkylation and deprotected afterwards, as above described. An alternative alkylation method comprises reacting such compounds with an appropriate aldehyde in the presence of a reducing agent, such as sodium cyanoborohydride, in a lower alkanol as solvent at from 20 to 100°C. - Compounds I in which R3 represents an alkanoylamino or alkylsulphonylamino group may be prepared by reaction of the corresponding compounds in which R3 represents an amino group with an appropriate alkanoyl or alkylsulphonyl halide or anhydride, in a non-protic solvent, such as chloroform, 1,2-dichloroethane, toluene, acetone or pyridine in the optional presence of a base such as potassium carbonate or triethylamine at from 20 to 120°C. - Compounds I in which R^ or R3 represents a hydroxy group may be prepared by desalkylation of the corresponding compounds in which R^ or R3 represents an alkoxy group. This can be accomplished by treatment with BBr3 dichloromethane at from 0 to 40°C (T.W. Greene, Protective Groups in Organic Synthesis, page 87, Wiley Interscience, 1981) or according to other methods described in the same reference. - Compounds I in which R^ represents an a-hydroxyalkyl group may be prepared by reduction of the corresponding 271634 | wx. jwrfctfi office -11- 1 5 FEB 1S96 RECEIVED compounds in which represents an alkanoyl group. Such reduction may be effected with sodium borohydride or sodium cyanoborohydride in a lower alkanol as solvent at from 0 to 90°C. Compounds I in which Y represents a group -NH- may be converted to corresponding compounds in which Y represents a group -N(R2)-, R2 being other than a hydrogen atoii. In particular, alkylation can be effected with an alkylating agent L-alkyl or with an aldehyde in reductive conditions, both methods being as described above. Acylation can be effected with an alkanoyl halide or anhydride as described above. Carbamoylation can be achieved by reacting the compound in which Y represents a group -NH- with an inorganic cyanate salt, e.g. potassium cyanate, in acetic acid at from 20 to 100°C. Likewise, in the same conditions or in non-protic solvents such as toluene, chloroform or DMF, the compounds in which R2=alkylcarbamoyl can be formed using alkyl isocyanates. Compounds of formula I having Y=NH can be prepared by reduction of the corresponding compounds I in which Y=NHC0 and W has one less carbon atom, the reduction suitably being performed with lithium aluminium hydride or other hydrides, such as borane, or mixed hydrides, operating in aprotic solvents (e.g., diethyl ether, tetrahydrofuran) at from 20 to 60°C, as described by J. March in Advanced organic Chemistry, IV Ed, page 1219, Wiley Interscience, 1992. N-oxides of the compounds of the invention may be formed by oxidising the basic nitrogen atom(s) present, mainly, but not necessarily only, that at position 1 of the piperazine ring. The oxidation can be performed by reacting compounds of formula I with hydrogen peroxide at from 20 to 60°C in acetic acid or with m-chloroperbenzoic acid or magnesium mono-peroxyp.hthalate at from 0 to 50°C, according to Broughan et al., Synthesis, 11, 1015, 1987. Persons skilled in the art will be aware that all the above * 1 5 FED 13D5 f_, RECEIVED synthetic pathways might be simplified provided that the reacting intermediatepi do not bear further groups sensitive to the same reactants (for example : CO, NH2, NHAlk or OH groups). Compounds of formula I bearing such reactive groups can be prepared through the above paths provided that the reactive groups present in the starting materials are protected beforehand and then deprotected after the reaction. Several examples of protection and deprotection for various reactive groups can be found in T.W- Greene, Protective Groups in Organic synthesis, Wiley Interscience, 1991. Alternatively, unreactive groups (e.g. NO2) can be left unconverted during the first reaction and then converted to reactive ones (e.g. NH2) as a final step of the pathway. Which synthetic technique will be preferred depends on the compound desired to be synthesized. Additional synthetic methods will be apparent to those skilled in the art. Prodrugs as above defined may be prepared from the corresponding hydroxy compounds of the invention by the following methods: - by reaction with a chloroformate, an isocyanate or isothiocyanate, a carbonyl chloride or bromide or another activated acid derivative (e.g. an anhydride) in a suitable solvent (e.g. a chlorinated solvent, DMF, tetrahydrofuran, dioxane, acetonitrile, pyridine) in the presence or otherwise of a base such as triethylamine, pyridine, 4-dimethylaminopyridine, sodium hydroxide, potassium carbonate or 1,10-diazabicycloundec.ene at from -20 to 100°C; - by reaction with a carboxylic acid in one of the solvents above specified, in the presence of a condensing agent such as N,N1 -carbonyldiirnidazole or a carbodiimide; - by reactipn with a dialkyl or diaryl chlorophosphate or dialkyl cyanophosphonate in the same conditions described above (for examples of such derivatization methods see S.O. Thorberg et al., J. Wed. Chem., 30^, 2008, 1987). 271634 -12- 271634 -13- DETAILED PREPARATION OF INTERMEDIATES Ethyl 2-benzyloxy-3-propionylbenzoate Intermediate I N.Z. PATENT OFFIC 1 5 FEB 1996 RECEIVED A mixture of ll.l g of ethyl 2-hydroxy-3-propionylbenzoate, prepared as described in DE 2059296, 27.64 g of anhydrous potassium carbonate and 8.58 g of benzyl bromide in 250 ml of ethyl acetate was stirred at reflux for 4 hours. After cooling the reaction mixture to room temperature, insoluble matter was removed by suction filtration and the filtrate was evaporated to dryness yielding quantitatively the title compound, used without further purification (GLC assay was 99%). B.p. 195-205°C (2 mmHg). 2-Benzyloxy-3-propionylbenzoic acid Intermediate II A mixture of 4.68 g of Intermediate I and 90 ml of 2N sodium hydroxide in 38 ml of ethanol was stirred at 80°C for 2 hours. After this period, the organic solvent was removed by evaporation in vacuo. The residue was rinsed with 90 ml of water and extracted with diethyl ether; the aqueous layer was acidified (pH=l) by adding 37% HC1 and extracted with ethyl acetate. The organic layer was washed with water to neutrality, dried on sodium sulphate and evaporated to dryness in vacuo yielding the title compound, used without further purification. It melted at 65-67°C after repeated crystallization from petroleum etherrdiethyl ether 8:2. 2,2-D imethy1-3-[4-(2-methoxypheny1)-1-pipera z iny1]--propionaldeyde dihydrochloride Intermediate III A mixture of 3 g of 1-(2-methoxyphenyl)-piperazine dihydrochloride, 0.4 g of paraformaldehyde and 1 ml of isobutyraldehyde in 4 ml of ethanol was stirred at reflux 271634 N.Z. PATENT OFFICE 1 5 FEB 1996 -14- RECE1VED for 1.5 hours. 0.4 g of paraformaldehyde was added and the mixture was stirred again for 1% hours at reflux. After cooling to room temperature, water was added and the resulting solution was washed twice with diethyl ether, made alkaline by adding IN aqueous sodium hydroxide solution and extracted with diethyl ether. The organic layer was washed with water, dried on sodium sulphate and evaporated to dryness in vacuo. The crude product was purified by flash chromatography on silica gel eluting with ethyl acetate. Evaporation in vacuo of the collected fractions yielded 2.1 g of the base of the title compound. This was dissolved in diethyl ether and acidified with 3.8N hydrochloric acid in diethyl ether. The title compound was recovered by suction filtration and melted at 181-183°C, l- (2,2-Dimethyl-3-hydroxypropyl)-4-(2-methoxyphenyl)--piperazine dihydrochloride Intermediate IV 1.4 4 g of sodium borohydride was added portionwise at 0°C to a solution of 11.64 g of the base of Intermediate III in 90 ml of ethanol. The reaction mixture was stirred at room temperature for 24 hours. It was then cooled to 0°C, acidified to pH = 1 with 3N hydrochloric acid and extracted with ethyl acetate. The organic layer was washed with water, dried on sodium sulphate and evaporated to dryness in vacuo. The oily residue was purified by flash chromatography eluting with petroleum ether:ethyl acetate 6:4. Evaporation in vacuo of the collected fractions yielded 7.29 g of the base of the title compound, and this was converted in the usual manner into its hydrochloride salt. It melted at 203-205°C after crystallization from ethyl acetate, followed by acetonitrile. Benzyl 3-acetyl-2-benzyloxybenzoate Intermediate V A mixture of 3.5 g of 3-acetyl-2-hydroxybenzoic acid | N.Z. r'Ai'izriT Gr.'ICE 271634 1 5 FEB 1998 _15_ , RECEIVED (prepared as described by R.E. Ford., J. Med.- Chem., 29, 538, 1986), 5.3 ml of benzyl bromide and 4.17 g of anhydrous potassium carbonate in 40 ml of anhydrous DMF was stirred at 80°C for 4 hours. After cooling to room temperature, the reaction mixture was poured into water and extracted with diethyl ether. The organic layer was dried on sodium sulphate, evaporated to dryness in vacuo and purified by flash chromatography, eluting with a petroleum ether:ethyl acetate 9:1 mixture. Yield: 6.05 g of the pure title compound. M.p. 42-47°C (n-hexane:cyclohexane 1:1). 3-Acetyl-2-benzyloxybenzoic acid Intermediate VI A mixture of 5.4 6 g of Intermediate V and 30 ml of IN sodium hydroxide in 75 ml of 95% ethanol was stirred for 7 hours at room temperature. The solvent was evaporated off in vacuo and the residue was diluted with water and acidified (pH=l) with IN hydrochloric acid. Extraction with ethyl acetate followed by the usual procedure yielded 5.32 g of the pure title compound. 3-H-NMR spectrum at 60 MHz, CDCI3 (6) 10.6 (bs, 1H, COOH) 8.25 (dd, 1H, CH in position 6 of the benzoic acid) 7.90 (dd, 1H, CH in position 4 of the benzoic acid) 7.20-7.70 (m, 6H, CH in position 5 of the benzoic acid and benzyl CHs) 5.10 (s, 2H, CH2) 2.60 (s, 3H, CH3) 2-(4-Nitrobenzyloxy)-3-propionylbenzoic acid Intermediate VII A mixture of 2.2 g of ethyl 2-hydroxy-3-propionylbenzoate, 3.24 g of 4-nitrobenzyl chloride and 2.07 g of anhydrous potassium carbonate in 20 ml of anhydrous DMF was stirred at 80°C for 3 hours. After cooling to room temperature, the reaction mixture was poured into water and extracted with 911 63'4'N'z:pATE*TOFncg ^/ i 15 FEB 1996 -16- ; RECEIVED ethyl acetate. After the usual work-up, '"tTTe"~emae—was purified by flash chromatography eluting with a petroleum ether:ethyl acetate 8:2 mixture. Evaporation in vacuo of the solvents from the pooled fractions gave 2.84 g of pure ethyl 2-(4-nitrobenzyloxy) -3-propionylbenzoate, characterized by NMR spectroscopy, as follows. ^•H-NMR spectrum at 60 MHz, CDCI3 (5) 8.10-8.35 (m, 2H, CHs at positions 3, 5 of the nitrophenyl) 8.00 (dd. 1H, CH at position 6 of the benzoate) 7.50-7.80 (m, 3H, CHs at position 4 of the benzoate and at positions 2, 6 of the nitrophenyl) 7.3 (dd, 1H, CH at position 5 of the benzoate) 5.10 (s, 2H, CH2Ph) 4.35 (q, 2H, CH20) 2.90 (q, 2H, CH2CO) 1.15-1.35 (2xt, 6H, 2 X CH3) 2.42 g of the so obtained ester was hydrolyzed following the procedure described for Intermediate VI. Yield: 2.02 g of the title compound. M.p. 103-107°C (toluene:petroleum ether 1:1). Ethyl 2-(4-benzoylbenzyloxy)-3-propionylbenzoate Intermediate VIII A solution of 11.89 g of 4-methylbenzophenone, 12.82 g of N-bromosuccinimide and 0.3 g of benzoylperoxide in 30 ml of tetrachloromethane was refluxed for 6% hours. Filtration of the precipitated succinimide followed by evaporation to dryness of the mother liquor yielded 17.2 g of crude 4-benzoylbenzyl bromide (73% by NMR) used without further purification. The title compound was synthesized according to the first step of the method described for the preparation of Intermediate VII, but starting from 2.64 g of ethyl 2-hydroxy-3-propionylbenzoate and using 4.96 g of the 73% 271634 N.Z. PATENT OFFICE 1 5 FEB 1996 -17- t received 4-benzoylbenzyl bromide instead of 4-nitrobenzyl chloride. Purification by flash chromatography (eluent: petroleum ether:ethyl acetate 8:1) yielded 4.1 g of the pure title compound as an oil. Elemental Analysis for C26H24O5: Calc.: C, 74.98; H, 5.81. Found: C, 75.11; H, 5.82. 2-(4-Benzoylbenzyloxy)-3-propionylbenzoic acid Intermediate IX The title compound was prepared according to the procedure described for Intermediate VI but starting from Intermediate VIII instead of Intermediate V. M.p. 139-141°C (toluene). 2-Benzyloxy-N-(3-hydroxypropyl)-benzamide Intermediate X A solution of 22.83 g of 2-benzyloxybenzoic acid (prepared as described in J. Am. Chem. Soc., 6j5, 2140, 1943), 15.9 ml of thionyl chloride and 130 ml of ethanol-free chloroform was stirred at reflux temperature for 3% hours. After cooling to room temperature, the mixture was evaporated to dryness in vacuo to give 23.94 g of crude 2-benzyloxybenzoyl chloride, used without purification. A solution of the above intermediate in 100 ml of dichloiomethane was added dropwise at room temperature to a stirred solution of 8.4 g of 3-aminopropanol, 15.5 ml triethylamine and 100 ml of dichlorowethane. The mixture was stirred at 20-25°c for 2 hours, and then successively washed with 2N hydrochloric acid, water, 5% aqueous sodium bicarbonate, and water. The organic layer was dried over anhydrous sodium sulphate and evaporated to dryness in vacuo to give 26.6 g of the title compound. M.p. 117-119°C after crystallization from isopropyl acetate. -18- 2-Benzyloxy-N-(3-chloropropyl)-benzamide Intermediate XI 271634 N.Z. PATENT OFFICE r 1 5 FEB 1996 received A solution of 4.4 ml of thionyl chloride in 45 ml of ethanol-free chloroform was added dropwise within 20 minutes to a refluxing solution of 8.64 g of Intermediate X in 120 ml of ethanol-free chloroform. The mixture was stirred at reflux temperature for 3 hours and then cooled to room temperature. It was washed successively with water, 5% aqueous sodium bicarbonate, and water. The organic layer was dried over anhydrous sodium sulphate and evaporated to dryness in vacuo to give 10.3 g of the title compound whose structure was confirmed by NMR spectroscopy, as follows. NMR spectrum at 200 MHz, CDCI3 (S) 8.22 (dd, 1H, CH in position 6 of benzamide ring) 7.90-8.05 (bs, 1H, CONH) 7.38-7.46 (m, 6H, CH in position 4 of benzamide ring and aromatic CHs of benzyl ring) 7.02-7.10 (m, 2H, CHs in positions 3 and 5 of benzamide ring) 5.15 (s, 2H, 0CH2) 3.47 (q, 2H, NHCH2CH2CH2C1) 3.36 (t, 2H, NHCH2CH2CH2C1) 1.86 (m, 2H, NHCH2CH2CH2C1) DETAILED PREPARATION OF THE COMPOUNDS OF THE INVENTION Example 1 3-[4-(2-Methoxyphenyl)-l-piperazinyl]-propyl 2-benzyloxybenzoate dihydrochloride A mixture of 3.36 g of 2-benzyloxybenzoic acid (prepared as described in J. Am. chem. Soc., 65, 2140, 1943) and 2.08 g of anhydrous potassium carbonate in 40 ml of anhydrous DMF was stirred at 80°C for l hour. The reaction mixture then was cooled to room temperature, and 3.5 g of 271634 N.Z. PATENT OFFICE 1 5 FEB 1996 -i9- r ' .'.'ED 1-(3-chloropropyl)-4-(2-methoxyphenyl)-piperazine was added. The mixture was stirred at 50°C for 3 hours and then once again cooled to room temperature. It was poured into water and extracted with dichloromethane to give the crude base of the title compound. This crude base was dissolved in 50 ml of ethanol and 9 ml of 5N ethanolic hydrogen chloride was added. The crystals which precipitated were recovered by suction, yielding the title compound. M.p. 201-203°C (ethanol). Example 2 3- [4- (2-Methoxyphenyl) -1-piperazinyl] -propyl 2-methoxy-3-propionylbenzoate dihydrochloride The title compovmd was obtained following the procedure of Example l but starting from 2-methoxy-3-propionylbenzoic acid, (prepared as described in JP 6064944) instead of 2-benzyloxybenzoic acid, and extracting with ethyl acetate instead of dichloromethane. M.p. 192-193°C (ethanol). Example 3 3 — [ 4— (2-Methoxyphenyl) -1-piperazinyl] -propyl 2-benzyloxy-3-propionylbenzoate dihydrochloride The title compound was obtained following the procedure described in Example l but using Intermediate II instead of 2-benzyloxybenzoic acid. After conversion into its dihydrochloride, the title compound melted at 175-176°C (ethanol). Example 4 2-Benzyloxy-.N--{3-[4- (2-methoxyphenyl) -l-piperazinyl]--propyl}-benzamide dihydrochloride . 0.25-H2O 0.94 g of 1, l'-carbonyldiimidazole was added portionwise at 271634 -20- j RECEIVED 0°C to a stirred solution of 2.56 g of 2-benzyloxybenzoic acid in 25 ml of anhydrous tetrahydrofuran. Stirring was continued for 1 hour at the same temperature; afterwards 3.35 g of 1-(3-aminopropyl)-4-(2-methoxyphenyl)-piperazine (prepared as described in GB 2161807) was added and the reaction mixture was stirred at room temperature for a further l\ hours. The solvent was removed by evaporation in vacuo and the residue was rinsed with water and extracted with ethyl acetate. The organic layer was washed with water, dried on anhydrous sodium sulphate and evaporated to dryness in vacuo. The crude product was purified by flash chromatography on silica gel eluting with ethyl acetate, yielding 4.63 g of the title compound as a base, which was converted in the usual manner into its dihydrochloride, m.p. 179-180°C (ethanol). Example 5 2-Hydroxy-N-{ 3-[4 - (2-methoxyphenyl) -1-piperazinyl] -propyl} --3-propionylbenzamide dihydrochloride A solution of 7.48 g of 1-(3-aminopropyl)--4-(2-methoxyphenyl)-piperazine in 30 ml of dichloromethane was added dropwise over a period of 45 minutes at 4°C into a stirred mixture of 6.37 g of 2-hydroxy-3-propionylbenzoyl chloride (prepared as described in DE 2 631248) and 4.2 ml of triethylamine in 80 ml of dichloromethane. The reaction mixture was stirred for 2 hours at 4°C and for 8 hours at room temperature, and was then extracted with 5% aqueous sodium hydrogen carbonate solution followed by water. The organic layer was dried over anhydrous sodium sulphate. The residue obtained by evaporation in vacuo of the solvent was purified by flasn chromatography on silica gel eluting with dichloromethane:methanol graduated 98:2 to 95:5. Thfe fractions containing the pure base were pooled, the solvents were evaporated off in vacuo, the residue was dissolved in ethanol and an excess of ethanolic hydrogen chloride was added. Diethyl ether was added until crystallization of the N.Z. PAitrtT' 1 5 FEB 1995 271 634 -21- salt. 7.6 g of the title compound was obtained after recrystallization from methanol. M.p. 215-217°C with 3 —[4 —(2 —Methoxypheny1)-1-piperazinyl]-propyl ■ — 2-(2-chlorobenzyloxy)-benzoate monomethanesulphonate A mixture of 17.6 g of sodium salicylate, 26.9 g of 1-(3-chloropropyl)-4-(2-methoxyphenyl)-piperazine and 35 ml of acetonitrile was stirred at reflux temperature for 5 hours. After cooling to room temperature, the reaction mixture was poured into 500 ml of water and extracted with dichloromethane. The organic layer was separated) dried over anhydrous sodium sulphate and evaporated in vacuo giving 32.1 g of 3-[4-(2-methoxyphenyl)-l-piperazinyl]-propyl 2-hydroxybenzoate as a base. This was converted into its monomethanesulphonate by the usual method. M.p. 156-157°C (ethanol:diethyl ether). A mixture of 3.5 g of the product prepared above, 6.9 g of anhydrous potassium carbonate and 5 ml of acetonitrile was stirred at reflux temperature. After 15 minuted, a solution of 1.72 g of 2-chlorobenzyl chloride in 5 ml of acetc.nitrile was added dropwise and heating was continued for further 4 hours. After cooling to room temperature, the reaction mixture was diluted with 10 ml of water and extracted with 20 ml of dichloromethane. The organic layer was separated, dried over anhydrous magnesium sulphate, and evaporated to dryness in vacuo. The crude base was purified by flash chromatography, on silica gel eluting with acetone:petroleum ether 1:3. The pure base was dissolved in ethanol and one equivalent of a 2N ethanolic solution of methanesulphonic acid was added, followed by diethyl ether addition until crystallization of the salt. 3.4 g of the title compound was obtained after decomposition. Example 6 t 5 FEB 1996 RECEIVED 2716 -22- recrystallization from ethanol:diethyl ether 114-117 °C). (m.p. Example 7 3-[4-(2-Methoxyphenyl)-l-piperazinyl]-propyl 2-(3-chlorobenzyloxy)-benzoate monomethanesulphonate N.Z. 1 5 FEB 1996 received This compound was prepared according to the method described in Example 6 but using 3-chlorobenzyl chloride instead of 2-chlorobenzyl chloride and the reaction lasted 20 hours. The title compound was crystallized from ethanol:diethyl ether and melted at 140-143°C. Example 8 3-[4-(2-Methoxyphenyl)-1-piperazinyl]-propyl 2-(4-chlorobenzyloxy)-benzoate monomethanesulphonate hemihydrate This compound was prepared according to the method described in Example 6 but using 4-chlorobenzyl chloride instead of 2-chlorobenzyl chloride and the reaction lasted 16 hours. The title compound was crystallized from ethanol:diethyl ether and melted at 143-146°C. Example 9 3-[4-(2-Methoxyphenyl)-l-piperazinyl]-propyl 2-(2-methoxybenzyloxy)-benzoate monomethanesulphonate This compound was prepared according to Example 6 but using 2-methoxybenzyl chloride instead of 2-chlorobenzyl chloride and the reaction lasted 5 hours. The title compound was crystallized from ethanol:diethyl ether and melted at 117-119°C. 27163 -23- Example 10 3-[4-(2-Methoxyphenyl)-1-piperazinyl]-propyl 2-(3-methoxybenzyloxy)-benzoate monomethanesulphonate This compound was prepared according to Example 6 but using 3-methoxybenzyl chloride instead of 2-chlorobenzyl chlotide. The title compound was crystallized from ethanol:diethyl ether and melted at 125-126.5°C. Example 11 3-[4-(2-Methoxyphenyl)-l-piperazinyl]-propyl 2-(4-methoxybenzyloxy)-benzoate monomethanesulphonate hydrate This compound was prepared according to Example 6 but using 4-methoxybenzyl chic ride instead of 2-chlorobenzyl chloride and the reaction lasted 7 hours. The title compound was crystallized from ethanol:diethyl ether and melted at 128-132°C. Example 12 3-[4-(2-Methoxyphenyl)-l-piperaz iny1]-propyl 2-(2,3-dimethoxybenzyloxy)-benzoate monomethanesulphonate This compound was prepared according to Example 6 but using 2,3-dimethoxybenzyl chloride instead of 2-chlorobenzyl chloride and the reaction lasted 2 hours. The title compound was crystallized from ethanol:diethyl ether and melted at 141—143°C. N.Z. PATENT OFFICE 1 5 FEB 1996 received 271634 -24- Example 13 3-[4-(2-Methoxyphenyl)-l-piperazinyl]-propyl 2-(3,4-dimethoxybenzyloxy)-benzoate monomethanesulphonate This compound was prepared according to Example 6 but using 3,4-dimethoxybenzyl chloride instead of 2-chlorobenzyl chloride and the reaction lasted 2 hours. The title compound was crystallized from ethanol:diethyl ether and melted at 132-135°C. Example 14 3-[4-(2-Methoxyphenyl)-1-piperazinyl]-propyl 2- (3,4,5--trimethoxy-benzyloxy)-benzoate monomethanesulphonate 0.75-H20 This compound was prepared according to Example 6 but using 3,4,5-trimethoxybenzyl chloride instead of 2-chlorobenzyl chloride and the reaction lasted 2 hours. The title compound was crystallized from ethanol:diethyl ether and melted at 97-103 °C. Example 15 3— C 4—(2-Methoxyphenyl)-l-piperazinyl]-propyl 2-octyloxybenzoate monomethanesulphonate hydrate. This compound was prepared according to Example 6 but using 1-bromooctane instead of 2-chlorobenzyl chloride and the reaction lasted 15 hours. The title compound was crystallized from ethanol:diethyl ether and melted at 75-77 °C. U- N.Z. PATENT OFFICE \ 5 FEB TO 271634 -25- Example 16 3-[4-(2-Methoxypheny1)-1-piperaz iny1]-propyl 2-butoxybenzoate monomethanesulphonate hydrate This compound was prepared according to Example 6 but using 1-bromobutane instead of 2-chlorobenzyl chloride and" the reaction lasted 11 hours. The title compound was crystallized from ethanol:diethyl ether and melted at 69-72°C. Example 17 3-[4-(2-Methoxyphenyl)-1-piperazinyl]-propyl 2-cyclohexylmethoxybenzoate monomethanesulphonate hemihydrate This compound was prepared according to Example 6 but using cyclohexylmethyl bromide instead of 2-chlorobenzyl chloride and the reaction lasted 3 3 hours. The title compound was crystallized from ethanol: diethyl ether and r.elted at 104-106 °C. Example 18 3-[4-(2-Methoxyphenyl)-1-piperazinyl]-propyl 2-(2-naphthylmethoxy)-benzoate monomethanesulphonate hydrate This compound was prepared according to Example 6 but using 2-bromomethylnaphthalene instead of 2-chlorobenzyl chloride and the reaction lasted 30 hours. The title compound was crystallized from ethanol:diethyl ether and melted at 101-103 °C. N.Z. PATfcK i .. ; : 15 FEB 1996 r RECEIVED 271634 -26- Example 19 2, 2-Dimethyl-3-[4-(2-methoxypheny 1)-1-pipera ziny 1 ]-propy 1 2-benzyloxybenzoate dihydrochloride A mixture of 2.73 g of 2-benzyloxybenzoic acid, 0.97 ml of thionyl chloride and 0.10 ml of anhydrous DMF in 24 ml of anhydrous dichloromethane was stirred at room temperature for 2% hours under a nitrogen stream. After this period, the reaction mixture was cooled to 0°C and a solution of 3.67 g of Intermediate IV in 24 mi of anhydrous dichloromethane was added dropwise in 5 minutes. Stirring was continued for 22 hours at room temperature. 40 ml of a 0.4M aqueous solution of sodium carbonate was then added, followed by 20 ml of dichloromethane. The organic layer was separated'off, washed with water, dried on sodium sulphate and evaporated to dryness in vacuo. The oily residue was purified by flash chromatography on silica gel eluting with dichloromethane:ethyl acetate graduated from 100:3 to 100:7.5. Evaporation in vacuo of the collected fractions yielded 4.31 g of the base of the title compound. The base was dissolved in isopropanol and excess isopropanolic hydrogen chloride was added. Crystals were recovered by suction filtration yielding 3.82 g of the title compound, which melted at 188-190°C after recrystallization from acetonitrile. Example 20 N—{3—[4-(2-methoxypbeny1)-1-pipera z iny1]-propyl} -2-phenylthio-benzamide dihydrochloride 0.34 ml of diethyl cyanophosphonate was added dropwise at 0-5 °C to a stirred solution of 0.4 6 g of 2-phenyltbio-benzoic acid (prepared as described in J. Am. Chem. Soc., 84, 1561, 1962) and 0.55 g of 1-(3-aminopropyl)-4-(2-methoxyphenyl)-piperazine in 4 ml of anhydrous DMF. Immediately afterwards, 0.13 ml of N.Z. PATENT OFFICE 1 5 FEB 1996 RECEIVED 271634 -27- triethylamine was added dropwise at the same temperature. After 3 0 minutes stirring at 0-5 °C and l hour at room temperature, the reaction mixture was poured into 30 ml of water and extracted with ethyl acetate. The organic layer was washed with water, dried over anhydrous sodium sulphate and evaporated to dryness in vacuo. The crude product thereby obtained was dissolved in ethanol and 5N ethanolic hydrogen chloride was added. The title compound crystallized upon addition of diethyl ether. Yield: 0.61 g. M.p. 210-213°C. Example 21 2-Benzy1-N-{3-[4-(2-methoxypheny1)-1-piperaz iny1]-propyl}--benzamide dihydrochloride O.125-H2O The title compound was prepared by the method described in Example 20 but using 2-benzylbenzoic acid instead of 2-phenylthio-benzoic acid. It was purified by crystallization from ethanol:diethyl ether and melted at 184-188°C. Example 22 N-{3-[4-(2-Methoxyphenyl)-1-piperazinyl]-propyl}--2-phenethyl-benzamide dihydrochloride The title compound was prepared by the method described in Example 20 but using 2-phenethyl-benzoic acid instead of 2-phenyithic benzoic acid. It was purified by crystallization from ethanol:diethyl ether and melted at 186-189 °C. N.Z. PATENT OFFICE 1 5 FEB 1996 received 271634 -28- Example 23 2-Benzyloxy-N-{3-[4-(2-hydroxyphenyl)-1-piperazinyl]--propyl}-benzamide hydrochloride A mixture of 4.55 g of Intermediate XI, 2.1 g of anhydrous potassium carbonate and 2.7 g of l-(2-hydroxyphenyl)-piperazine was stirred at 185-190°C for 30 minutes. After cooling to room temperature, 100 ml of chloroform and 60 ml of 1% aqueous sodium bicarbonate solution were added to the mixture and vigorously stirred. The organic layer was separated, washed with water, dried over anhydrous sodium sulphate, and evaporated to dryness in vacuo. The residue was purified by flash chromatography on silica gel eluting with a chloroform:5N methanolic ammonia 100:1 mixture. The so obtained base of the title compound was dissolved in methanol, acidified by addition of 1 equivalent of 5N ethanolic hydrogen chloride solution, and evaporated to dryness in vacuo to give a glassy solid. This was stirred with acetone, and then filtered off and crystallized from isopropanol to give 3.26 g of the title compound. M.p. 197-199°C. Example 24 2-Methoxy-N-{3-[4-(2-methoxypheny1)-1-p ipera z iny1]-propyl}--3-propionylbenzamide hydrochloride I.I-H2O 2.0 ml of diethyl cyanophosphonate and 1.67 ml of triethylamine were added at 0°C to a solution of 2.08 g of 2-methoxy-3-propionylbenzoic acid in 50 ml of anhydrous DMF. 2.99 g of l-(3-aminopropyl)-4-(2-methoxyphenyl)-piperazine was then added. The reaction mixture was stirred at 0°C for 30 minutes and at room temperature for 2 hours, poured into water and extracted with ethyl acetate. The organic layer was dried on sodium sulphate and evaporated to dryness in vacuo. The residue was purified by flash chromatography, eluting with a dichloromethane:methanol 95:5 mixture, to N.Z. PATENT OFFICE 1 5 FEB 1996 RECEIVED 271634 -29- give the base of the title compound. This was converted by the usual procedure into its hydrochloride which was crystallized from ethyl acetate yielding 2.4 g of the title compound. M.p. 133-134°C. Example 25 3-Acetyl-2-benzyloxy-N-{3-[4-(2-methox^phenyl) --1-piperazinyl]-propyl}-benzamide dihydrochloride^ n.2. PATENT OFFICE 15 FEB 1996 received A solution of 4.35 g of Intermediate VI, 1.41 g of N-hydroyysuccinimide and 2.54 g of N,N'-dicyclohexyl--carbodiimide in 4 0 ml of anhydrous DMF was stirred for 2 hours at room temperature. After resting overnight, N,N1-dicyclohexylurea was filtered off by suction and 3.07 g of l-(3-aminopropyl)-4-(2-methoxyphenyl)-piperazine was added to the filtrate. After 3 hours stirring at room temperature, the reaction mixture was poured into water and extracted with ethyl acetate. Following the usual work-up, the product was purified by flash chromatography (eluent ethyl acetate:methanol 95:5) yielding the base of the title compound. This was dissolved in dichloromethane and 2 molar equivalents of 0.92N ethanolic hydrogen chloride were added. Evaporation to dryness in vacuo and crystallization from acetonitrile gave 4.2 g of the title compound. M.p. 163-164 °C. Example 26 2-Benzyloxy-N-{3-[4-(2-methoxyphenyl)-1-piperazinyl]--propyl}-3-propionylbenzamide dihydrochloride The title compound was obtained according to the procedure described in Example 25 but starting from 2.84 g of Intermediate II instead of Intermediate VI. 4.23 g were obtained, melting at 170-174°C (isopropanol). Z/1654 -30- Example 27 N- { 3 - [ 4 - (2-Methoxyphenyl) -l-piperaz iny1 ] -propyl} -2 --(4-nitrobenzyloxy)-3-propionylbenzamide dihydrochloride hydrate A solution of 1.43 g of Intermediate VII, 1.19 g of 1-(3-aminopropyl)-4-(2-methoxyphenyl)-piperazine, 0.8 g of diethyl cyanophosphonate and 0.67 ml of triethylamine in 21 ml of DMF was stirred at 0°C for 30 minutes and at room temperature for 2 hours, poured into water and extracted with ethyl acetate. After the usual work-up, the crude was purified by flash chromatography, eluting with ethyl acetate:methanol 95:5. This gave 2.15 g of the base of the title compound, which was converted into its hydrochloride and crystallized from acetonitrile. M.p. 164-170°C. Example 28 N-{3-[4-(2-Methoxyphenyl)-l-piperazinyl]-propyl}--2-(4-nitrobenzyloxy)-benzamide A mixture of 3.69 g of 3-[4-(2-methoxyphenyl)--1-piperazinyl]-propyl 2-hydroxybenzamide (prepared as described in EP 104614), 2.1 g of 4-nitrobenzyl chloride and 1.39 g of potassium carbonate in 50 ml of acetone was stirred at reflux for 3 hours. After cooling to room temperature, the reaction mixture was diluted with water. The solid which precipitated was collected by suction and purified by flash chromatography, eluting with ethyl acetate:methanol (graduated from 100:2.5 to 100:5). The title compound thus obtained was recrystallized from ethanol, yielding 3.19 g, melting at 123-125°C. 271634 -31- Example 29 2-(4-Benzoylbenzyloxy)-N-{3-[4-(2-methoxyphenyl)-1--piperazinyl]-propyl}-3-propionylbenzamide dihydrochloride The title compound was prepared according to Example 27 but starting from 3.03 g of Intermediate IX instead of Intermediate VII. Yield 3 g; m.p. 189-194°C (ethanpJ4- Example 30 2-(4-Benzoylbenzyloxy)-N-{3-[4-(2-methoxyphenyl)--1-piperazinyl]-propyl}-benzamide hydrochloride hydrate n.z. patent OFFICE 15 FEB 1996 received The title compound was prepared according to the procedure of Example 28, but using 73% 4-benzoylbenzyl bromide (prepared as reported in Intermediate VIII) instead of 4-nitrobenzyl chloride and DMF as a solvent, stirring at room temperature. The crude was purified by flash chromatography eluting with an ethyl acetate:methanol mixture graduated from 85:5 to 90:20, yielding the base of the title compound. This was dissolved in isopropanol and converted to its hydrochloride by adding 5.8N isopropanolic hydrogen chloride. The solid was filtered and recrystallized from aqueous 0.IN hydrochloric acid. M.p. 165-169°C. Example 31 N-{3-[4-(2-Methoxyphenyl)-l-piperazinyl]-propyl}--2-phenylethoxy-benzamide dihydrochloride A mixture of 3.7 g of N-{3-[4-(2-methoxyphenvl)- T -1-piperazinyl]-propyl}-2-hydroxy-benzamide, 4.14 g of anhydrous potassium carbonate, 4.8 ml of phenethyl bromide, 0.05 g of potassium iodide and 30 ml of anhydrous DMF was stirred at room temperature for 8 hours. After cooling to room temperature, the mixture was evaporated to dryness in vacuo, diluted' with water and extracted with chloroform. The 271634 -32- nrgp.nic layer was washed with water, dried over anhydrous sodium sulphate, and evaporated to dryness in vacuo. The residue was purified by flash chromatography on silica gel, eluting with chloroform:5N methanolic ammonia 100:1. The crude base was dissolved in ethanol and acidified with 5N ethanolic hydrogen chloride. Addition of diethyl ether gave a solid which was recrystallized from acetonitrile, yielding 1.12 g of the title compound, m.p. 165-166°C. Example 3 2 ?. -Benzy loxy-N- { 3 - [ 4- (2-methoxyphenyl) - l-piperaz iny 1 ] --propyl}-phenylacetamide The title compound was prepared according to the procedure described in Example 27 but starting from 2.08 g of 2-benzyloxy-phenylacetic acid (prepared as described in J. Am. Chew. Soc., 64_, 3051, 1942) instead of Intermediate VII. It was crystallized from diisopropyl ether. Yipld 3.4 a: m.p. 98-104°C. Example 33 N.Z. PATEM7 OFFICE 1 5 FEB 1996 received 2-Benzyloxy-N-{3-[4-(2-methoxyphenyl)-l-piperazinyl] --propyl}-N-methyl-benzamide dihydrochloride 0.64 ml of thionyl chloride was added to a solution of 1.82 g of 2-benzyloxybenzoic acid in 10 ml of dichloromethane and 0.064 ml of anhydrous DMF. After 1% hours stirring at room temperature, the reaction mixture was cooled to 0°C and 2.4 g of 1-(2-methoxyphenyl)-4-(3-methylamino-propyl)-piperazine in 16 ml of dichloromethane was added. After 5 hours stirring at room temperature, the reaction mixture was diluted with 4% sodium carbonate. The organic layer was separated off, dried on sodium sulphate and evaporated to dryness in vacuo. The crude was purified by flash chromatography (eluent chloroform:methanol 100:4) giving the base of the title compound. This was dissolved in 271634 -33- isopropanol. 5.8N isopropanolic hydrogen chloride was added; the precipitated solid was collected by suction filtration and recrystallized from acetonitrile yielding 1.5 g of the title compound melting at 184-187°C. Example 34 N-{3-[4-(2-Methoxypheny1)-1-p ipera z iny1]-propy1}--2-(3-phenylpropoxy)-benzamide The title compound was prepared by the method described in Example 31 but using 3-phenylpropyl bromide instead of phenethyl bromide. The crude product was purified by crystallization from isopropanol; m.p. 86-88°C. Example 35 0-Ethyl-N-{3-[4-(2-methoxyphenyl)-1-piperazinyl]-propyl}--phenylphosphonamide A solution of 2.04 g of 0-ethyl phenylphosphonyl chloride (prepared as described in Phosphorus and Sulphur, 29, 169, 1987) in 70 ml of anhydrous diethyl ether was added dropwise at 5-15 °C to a stirred solution of 4.98 g of 1-(3-aminopropyl)-4-(2-methoxyphenyl)-piperazine in 50 ml of anhydrous dichloromethane. After stirring at room temperature for 2% hours, the precipitate was filtered and extracted with 70 ml of a 4:3 mixture of diethyl ether:dichloromethane, which then was evaporated to dryness in vacuo. The residue was purified by flash chromatography on silica gel eluting with dichloromethane: 5N methanolic ammonia (100:3) to give 3.67 g of the title compound as a thick oil. N.Z. PATENT OFFICE 1 5 FEB 1996 received frdr^r 27163^ NMR spectrum at 200 MHz, CDC13 (5) ■ w w ' 7.70-7.90 (m, 2H, CHs in positions 2 and 6 of P-phenyl ring) 7.30-7.55 (m, 3H, CHs in positions 3, 4 and 5 of P-phenyl ring) 6.75-7.05 (m, 4H, CHs of methoxyphenyl ring) 3.90-4.25 (m, 3H, 0CH2CH3 and NH) 3.85 (S, 3H, OCH3) 2.85-3.15 (m, 6H, CH2S in positions 3 and 5 of piperazine and PNHCH2CH2CH2) 2.50-2.68 (m, 4H, CH2s in positions 2 and 6 of piperazine) 2.47 (t, 2H, PNHCH2CH2CH2) 1.55-1.75 (m, 2H, PNHCH2CH2CH2) 1.3T (t, 3H, 0CH2CH3) Example 3 6 N-{3-[4-(2-Methoxyphenyl)-1-p ipera z iny1]-propyl}-2-phenoxy--benzamide hydrochloride . 0.25 H20 The title compound was prepared by the method described in Example 20 but using 2-phenoxybenzoic acid instead of 2-phenylthiobenzoic acid. The title compound melted at 176-182°C after crystallization from a 1:4 mixture of ethanol:diethyl ether. Example 37 N-{3-[4-(2-Methoxyphenyl)-l-piperazinyl]-propyl}-2-phenyl--benzamide 1.85-HC1 0.25-H20 The title compound was prepared by the method described in Example 2 0 but using 2-phenylbenzoic acid instead of 2-phenylthiobenzoic acid. The title compound melted at 192-195°C after crystallization from ethanol and resulted as a 1:0.425 mixture of monohydrochloride and dihydrochloride. W.Z. PATENT OFFICE 15 FEB 1998 I RECEIVED 271634 -35- Elemental Analysis for C27H31N3O2 1.85-HCl O.25-H2O Calc.: C, 64.66; H, 6.70; N, 8.38; Cl, 13.01; H20, 0.90. Found: C, 64.49; H, 6.63; N, 8.20; Cl, 12.81; H20, 0.74. PHARMACOLOGICAL DATA The receptor binding studies, as well as the experimental data on dogs reported below establish the compounds of the invention as a^-blockers, i.e., to be within a class of substances widely used as agents that can be used fo the relief of symptoms associated with obstructive disorders of the lower urinary tract, including (but not limited to) benign prostatic hypertrophy (BPH), and as antihypertensives. See, e.g., W. H. Frishman et al., Medical Clinics Of N. America, 72427, 1988 and rafprennes therein. Methodology N.Z. PATENT OFFICE 1 5 FEB 1996 RECEIVED Male Sprague Dawley rats [Crl: CD' BR] of 200-300 g b.w., female Albino Swiss mice [Crl: CD-I (ICR) BR] 20-30 g b.w., and male Beagle dogs (10-12 kg b.w.) were obtained from Charles River, Italy and Nossan (Correzzana, Milan, Italy), respectively. Animals were housed with free access to food and water and maintained on forced light-dark cycle at 22-24°C until the day of experiments. Acute toxicity The acute toxicity of the compounds of the invention was evaluated in female Albino Swiss mice after intraperitoneal and oral administration. Four logarithmic scaled doses of the compounds were dissolved or suspended in 0.5% Methocel and each dose was administered in a volume of 10 ml/kg to groups of 4. mice. Mortality was recorded 7 days after the administration. Data analysis : the LD50 values and their fiducial limits were calculated according to the method of Weil (Biometrics, 2716 -36 8, 249, 1952). Receptor Binding Studies The affinity of the compounds of the invention for the a^-adrenoceptor was investigated by utilizing [3H]prazosin as radioligand and rat hippocampal membranes, after irreversible inactivation of aig-adrenoceptors with chloroethylclonidine, as source of the aiA-adrenoceptors (c. Han et al., Mol. Pharmacol., 32, 505, 1987). Male rats were killed and their hippocampus was dissected, immediately frozen on dry ice and then stored at -70°C until use. These tissues were homogenized (2 x 20 sec) in 50 vols of 50 mM Tris-HCl buffer, pH 7.4. The homogenates were'centrifuged at 49000 x g for 10 min and the pellets were resuspended in 50 vols of the ice-cold buffer, incubated for 30 min at 37°C in the presence of 10 fiH chloroethylclonidine (CEC) and recentrifuged at 49000 x g for 10 min. The pellets were washed once more in the ice-cold buffer without CEC. The final pellets were suspended in 80-120 vols of 50 xnM Tris-HCl buffer, pH 7.4, containing 10 /iM pargyline and 0.1% ascorbic acid. The homogenates were incubated for 3 0 min at 25°C with 0.25-0.35 nM [3H]prazosin in the absence or presence of the displacing compound to be tested, concentration ranging from 10-4 to 5xl0~12 M, in a final volume of 2 ml. Non-specific binding was defined by 10 jxM phentolamine. The incubations were terminated by vacuum filtration over 0.2% polyethyleneimine pretreated Whatman GF/B fiber filters using Brandel cell harvester. The filters were then washed with 3x3 ml of ice-cold buffer and radioactivity retained on the filters was counted in 10 ml of Filter Count (Packard) in a liquid scintillation spectrometer with a counting efficacy of- 40%. The ability of the tested compounds to displace the specific [3H]prazosin binding was estimated from the IC50 value, which is the molar concentration of unlabelled compound necessary to displace 50% of the 1 5 FEB 1996 received 271634 j 1 5 FEB 1996 i -37- ( | received specific binding. The IC50 values were estimated by the non-linear curve-fitting program Allfit (A. De Lean et al., Am. J. Physiol., 235, E97, 1978). Effects on Urethral Contractions and Blood Pressure in Dogs The experiments were performed according to the method of Imagawa et al. (J. Pharmacol. Methods, 22_, 103, 1989), with substantial modifications, as follows: adult male beagle dogs, weighing 8-10. Kg, were anaesthetized with pentobarbital sodium (30 mg/Kg i.v. and 2 mg/Kg/h i.v.), intubated for artificial breathing getting air from room. In order to monitor systemic blood pressure (BP), a PE catheter was introduced into the aortic arch through the right common carotid artery. A collateral of the left femoral vein was cannulated for infusion of anaesthetic, and the right femoral vein was cannulated for administration of drugs. For intrarterial (i.a.) injection of noradrenaline (NA), a PE catheter was introduced into the lower portion of the abdominal aorta via the right external iliac artery. Through such a procedure, NA was selectively distributed to the lower urinary tract. Via a midline laparotomy, the urinary bladder and proximal urethra were exposed. In order to prevent the filling of the bladder, the two ureters were cannulated and the urine led outside. In order to record the prostatic urethral pressure, a Mikro-tip catheter (6 F) was introduced into the bladder via the external urethral meatus, and withdrawn until the pressure transducer was positioned in the prostatic urethra. A ligature was secured between the neck of the bladder and urethra to isolate the response of the latter and avoid any interaction with the bladder. Another ligature was put around the Mikro-tip catheter at the external urethral meatus, to secure the catheter itself. After a stabilizing period following surgical procedure (30 min), in which arterial and prostatic urethral pressure were continuously monitored as basal values, i.a. administration of NA was made at intervals of 10 minutes. The dose of NA chosen was 271634 -38- such to produce an increase of at least 100% in urethral pressure. The test compounds were administered i.v. in a cumulative manner with intervals of 15-20 min between administrations. I.a. injections of NA were repeated approximately 5 minutes after every dosing of test compound. Dose response curves were constructed computing the percentage inhibition to the increase in urethral pressure (NA induced), and the percentage drop in blood pressure produced by the test compound. ED25 for diastolic blood pressure (dose inducing a 25% decrease) and id50 (dose inducing a 50% inhibition of NA-induced increase in urethral pressure) were computed by means of linear regression analysis. Results Compounds as prepared in the Examples were tested according to the methods reported above, and the results are given in the Tables below, together with comparative results for the reference standards used. Compounds having receptor affinity (ic50 values) lower than about 500 nM are generally considered to have good affinity. Compounds with IC50 values less than 100 nM are generally preferred. 271634 -39-TABLE I Affinity for the ai a-adrenoceptor and acute toxicity data Compound Example No. ai&-Adrenoceptor Binding IC50 (nM) Acute in LD50 i.p. Toxicity Mice (mg/kg) p. 0. 1 7 315 1915 2 8 250 1571 3 13 220 1426 4 8 59 539 5 135 80 383 6 13 >500 2000 7 8 >500 >2000 8 20 >500 >2000 9 11 >500 >2000 10 23.5 >500 >2000 11 12 >500 >2000 12 22 500 >2000 13 >500 14 >500 >2000 15 >500 >2000 16 366 >2000 17 >500 >2000 18 >500 >2000 19 500 20 71 21 72 22 74 23 5 85 24 91 Prazosin Urapidil 1.4 435 429 1852 726 ' ^office J 1 5 FEB 1898 271634 N.2. PATENT OFFICE J -40- 1 5 FEB 1996 TABLE II Effects on Urethral Contractility and Blood P :essur$£Q§{VED Dogs Compound Example No. Urethra ED50 (Mg/kg) DBP ED25 (Mg/kg) DBP/Urethra ratio 1 4 52 23 349 377 6.7 16.4 Prazosin Urapidil 3.6 60.0 6.6 155.0 1.8* 2.6* Urethra: active dose in inhibiting by 50% the noradrenaline induced contraction of urethra DBP: active dose in lowering diastolic blood pressure by 25% DBP/urethra: ratio between the active doses (selectivity index) *) non-selective: substantial effect on both urethra and DBP Effective Amounts The following represent guidelines to effective oral, parenteral or intravenous dose ranges expressed in mg/kg of body weight per day for the following uses: In obstructive disorders of the lower urinary tract: General Preferred Most Preferred Most preferred As antihypertensives: General Preferred Most Preferred Most Preferred 0.02 - 40 0.1 - 2 0.6 (oral dose) 0.006 - 0.06 (intravenous dose) 0.02 - 40 0.2 - 10 2 (oral dose) 0.02 - 0.2 (intravenous dose) Selective use dosages, i.e. dosages that are active in the lower urinary tract without a substantial effect on the 271634 -41- M.i. PA t c!«i I GrriCE. 1 5 FEB 1996 received blood pressure depend on the particular compound employed but, generally, up to four times the ED50 of a selective compound can be administered without substantial effect on blood pressure. Further refinements and optimization of dosages are possible using no more than routine experiments. The active compounds of the invention may be orally administered, for example, with an inert diluent or with an edible carrier, or they may be enclosed in gelatin capsules, or they may be compressed into tablets. For the purpose of oral therapeutic administration, the active compounds of the invention may be incorporated with excipients and used in the form of tablets, troches, capsules, elixirs, suspensions, syrups, wafers, chewing gum and the like. These preparations should contain at least 0.5% of active compounds, but the amount of active ingredient may be varied depending upon the particular form and may conveniently be from about 5% to about 70% of the weight of the unit. The amount of active compound in such compositions is such that a suitable dosage will be obtained although the desired dosage can be obtained by administering a plurality of dosage forms. Preferred compositions and preparations according to the invention are prepared so that an oral dosage unit form contains from 2 to 600 mg of active compound. The tablets, pills, capsules, troches and the like may optionally contain any of the following ingredients: a binder such as micro-crystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose; a disintegrating agent such as alginic acid, Primogel, cornstarch and the like; a lubricant such as magnesium stearate or Sterotex; a glidant such as colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin; or a flavouring agent such as peppermint, methyl salicylate, or orange flavouring. When the dosage unit form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier such as a fatty oil. Other dosage unit forms may contain other various materials which modify the physical form of the dosage unit, for example, as coatings. </div>

Claims

<div class="application article clearfix printTableText" id="claims"> 271634 -42- Thus, tablets or pills may be coated with sugar, shellac, or other enteric coating agents. A syrup may contain, in addition to the active compounds, sucrose as a sweetening agent and certain preservatives, dyes, colourings and flavours. Materials used in preparing these various compositions should be pharmaceutically acceptable and non-toxic in the amounts used. For the purpose of parenteral therapeutic administration, the active compounds of the invention may be incorporated into a solution or suspension. These preparations should contain at least 0.2% of active compound, but may be varied between 0.5 and about 30% of the weight thereof. The amount of active compound in such compositions is such that a suitable dosage will be obtained. Preferred compositions and preparations according to the present invention are prepared so that a parenteral dosage unit contains from 0.4 to 200 mg of active compound. The solutions or suspensions may also include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulphite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates; citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. The parenteral multiple dose vials may be of glass or plastics material. Additional compositions suitable for administration by various routes and containing compounds according to the invention are also within the scope of the invention. Dosage forms, additional ingredients and routes of administration contemplated herein include those disclosed in US 4089969 and 5091182. N Z. PATENT OFFICE 1 5 FEB 1996 received 271634 -43- \VHAT WE CLAIM IS;-1. A compound of wherein Y represents a valence bond or one of the following groups, each of which is depicted with its left hand end being the end which attaches to the phenyl group and its right hand end being the end which attaches to the group W: -S(0)m-, -n(R2)-, -N(R2)CO-, -PO(OC2H5)NH-, -CO-, -so2N(R2)-, -(CH2)nC00- and -(CH2)nCON(R2)-, wherein m is 1 or 2, n is 0 to 2, and R2 represents a hydrogen atom, an alkyl group having up to 4 carbon atoms, a carbamoyl group, or an alkanoyl or alkylcarbamoyl group each having from 2 to 5 carbon atoms; W represents a linear or branched alkylene group having from 2 to 6 carbon atoms; A represents an alkoxy, alkyl or hydroxy group; A' represents one or more hydrogen or halogen atoms or alkoxy, alkyl or hydroxy groups; R represents a group of the formula -X-(CH2)p-Z, wherein X represents a valence bond or one of the following groups, each of which is depicted with its left hand end being the end which attaches to the phenyl group and its right hand end being the end which attaches to the group -(CH2)p-Z: -0-, -S(0)n-, -CO-, -N(R2)CO- and -N(R2)S02-; wherein n and R2 are as above defined; 27163 -44- p is 0 to 10, and Z represents a hydrogen atom, a cycloalkyl group having from 4 to 8 carbon atoms, a 1-naphthyl group, a 2-naphthyl group, a diphenylmethyl group or one of the groups having the following formulae r3 r3 O- -€>- CH=CH— wherein R3 represents one or more hydrogen or halogen atoms or cyano, hydroxy, alkoxy, alkyl, trifluoromethyl, alkanoyl, a-hydroxyalkyl, nitro, amino, alkylamino, dialkylamino, alkanoylamino, alkylsulphonylamino, phenyl, phenoxy or benzoyl groups; Rl represents a hydrogen or halogen atom or a cyano, hydroxy, alkyl, trifluoromethyl, alkanoyl, a-hydroxyalkyl, phenyl, phenoxy or benzoyl group; with the provisos that: when Y represents a valence bond or one of the groups ~S(O)m-, -N(R2)-, -S02N(R2)-, —CH2CON(R2) - or -CH2CH2C0N(R2)—, Z does not represent a hydrogen atom unless p is greater than 3; and when Y represents one of the groups -N(R2)C0-, -CO-, -(CH2)nC00_ or -C0N(R2)-, the group R, not being a hydrogen atom, is in the ortho position relative to the moiety Y and either (i) the group R^, not being a hydrogen atom, is in the ortho position relative to the group R or (ii) Z does not represent a hydrogen atom unless p is greater than 3 or a prodrug, enantiomer, diastereoisomer, crystalline form,. solvate, N-oxide or pharmaceutically acceptable salt of such a compound. OFFICE 1 5 FEB 1996 RECEIVED 271634 -45-
2. A compound according to claim 1 in which Y represents a group -COO- or -CONH-, depicted as stated in claim 1.
3. A compound according to claim 1 or claim 2 in which W represents a trimethylene group.
4. A compound according to any preceding claim in which a represents a methoxy group and A' represents a hydrogen atom.
5. Any one of the following compounds: 3-[4-(2-methoxyphenyl)-1-piperazinyl]-propyl 2-benzyloxy--benzoate, 3-[4-(2-methoxyphenyl)-l-piperazinyl]-propyl 2-methoxy--3-propionylbenzoate, 3-[4-(2-methoxyphenyl)-1-piperazinyl]-propyl 2-benzyloxy--3-propionylbenzoate, 2-benzyloxy-N-{3-[4-(2-methoxyphenyl)-1-piperazinyl]--propyl>-benzamide, 2-hydroxy-N-{3-[4-(2-methoxyphenyl)-l-piperaz iny1]-propyl>--3-propionylbenzamide, 3-[4-(2-methoxyphenyl)-l-piperaz iny1]-propyl 2-(2-chlorobenzyloxy)-benzoate, 3-[4-(2-methoxyphenyl)-l-piperaz iny1]-propyl 2-(3-chlorobenzyloxy)-benzoate, 3-[4-(2-methoxyphenyl)-1-piperazinyl]-propyl 2-(4-chlorobenzyloxy)-benzoate, 3-[4-(2-methoxyphenyl)-l-piperazinyl]-propyl 2-(2-methoxybenzyloxy)-benzoate, 3-[4-(2-methoxyphenyl)-1-piperazinyl]-propyl 2-(3-methoxybenzyloxy)-benzoate, 3-[4-(2-methoxyphenyl)-l-piperazinyl]-propyl 2-(4-methoxybenzyloxy)-benzoate, 3-[4-(2-methoxyphenyl)-l-pipera z iny1]-propyl 2-(2,3-dimethoxybenzyloxy)-benzoate, 3-[4-(2-methoxyphenyl)-l-pipera z iny1]-propyl 2-(3,4-dimethoxybenzyloxy)-benzoate, 3-[4-(2-methoxyphenyl)-l-piperaziny1]-propyl N.2. patent office 1 5 FEB 1996 received 271634 -46- 2-(3,4,5-trimethoxybenzyloxy)-benzoate, 3-[4-(2-methoxyphenyl)-l-piperaz iny1]-propyl 2-octyloxybenzoate, 3-[4-(2-methoxypheny1)-1-piperaziny1]-propy1 2-butoxybenzoate, 3-[4-(2-methoxyphenyl)-l-piperaz iny1]-propyl 2-cyclohexylmethoxybenzoate, 3-[4-(2-methoxyphenyl)-1-piperazinyl]-propyl 2-(2-naphthylmethoxy)-benzoate, 2,2-dimethyl-3-[4-(2-methoxyphenyl)-1-piperazinyl]-propyl 2-benzyloxybenzoate, N-{3-[4-(2-methoxyphenyl)-l-pipera z iny1]-propyl>--2-phenylthio-benzamide, 2-benzy1-N-{3-[4-(2-methoxyphenyl)-1-piperazinyl]-propyl}--benzamide, N-{3-[4-(2-methoxyphenyl)-l-piperaz iny1]-propyl>--2-phenethyl-benzamide, 2-benzyloxy-N-{3-[4-(2-hydroxyphenyl)-1-piperazinyl]--propyl>-benzamide, 2-methoxy-N-{3-[4-(2-methoxyphenyl)-l-piperaz iny1]-propyl}--3-propionylbenzamide, 3-acety1-2-benzyloxy-N-{3-[4-(2-methoxyphenyl)--1-piperazinyl]-propyl}-benzamide, 2-benzyloxy-N-{3-[4-(2-methoxyphenyl)-l-piperaz iny1]--propyl}-3-propionylbenzamide, N-{3-[4-(2-methoxyphenyl)-1-piperazinyl]-propyl}--2-(4-nitrobenzyloxy)-3-propionylbenzamide, N-{3-[4-(2-methoxyphenyl)-1-piperazinyl]-propyl}--2-(4-nitrobenzyloxy)-benzamide, 2-(4-benzoylbenzyloxy)-N-{3-[4-(2-methoxyphenyl)-1--piperaz iny1]-propyl}-3-propionylbenzamide, 2-(4-benzoylbenzyloxy)-N-{3-[4-(2-methoxyphenyl)--l-piperaz iny1]-propyl}-benz amide, N-{3-[4-(2-methoxyphenyl)-1-piperazinyl]-propyl}-2--phenylethoxy-benzamide, 2-benzyloxy-N-{3 — [4—(2-methoxyphenyl)-l-piperaz iny1]--propyl}-phenylacetamide, 2-benzyloxy-N-{3-[4-(2-methoxyphenyl)-1-piperazinyl]- N.il i'dNT OFFfCi 1 5 FEB 1996 RECEIVED 271634 -47- -propy1>-N-methy1-benzamide, N-{3-[4-(2-methoxyphenyl)-1-piperazinyl]-propyl}--2-(3—phenylpropoxy)-benzamide, 0-ethyl-N-{3-[4-(2-methoxyphenyl) -l-piperazinyl]-propyl}--phenylphosphonamide, N- { 3 - [ 4 - (2 -methoxyphenyl) -1-piper az iny 1 ] -propyl} -2 -phenoxy--benzamide, and N-{ 3- [ 4- (2-methoxyphenyl) -l-piperaziny 1 ] -propyl} -2-pheny1--benzamide; or a pharmaceutically acceptable salt thereof.
6. A pharmaceutical composition comprising a compound according to any preceding claim, or a prodrug, enantiomer, diastereoisomer, crystalline form, solvate, N-oxide or pharmaceutically acceptable salt of such a compound, in admixture with a pharmaceutically acceptable diluent or carrier.
7. A compound as claimed in any one of the preceding claims and substantially as hereinbefore described and with reference to any one of the Examples. RECORDATI SA. . CHEMICAL AND PHARMACEUTICAL COMPANY by their authorised agents, P L BERRY & ASSOCIATES. n.Z. PATENT office 1 5 FEB 1996 received </div>