KR19990009598A - Method for preparing indigestible high-lining texturine with high enzyme resistance - Google Patents

Abstract

The present invention relates to a method for preparing an indigestible dextrin having high enzyme resistance.

More specifically, the present invention relates to a method for preparing a high-enzymatic refractory dextrin by preparing heat-treated dextrin (pyrodextrin) using a starch material, followed by enzymatic treatment, filtration, decolorization, desalting and drying. The indigestible dextrin of the present invention is pre-drying the general starch with hot air, and then reacting a certain amount of anhydrous hydrochloric acid uniformly with the starch particles with a hot hot air and heat-treated for a certain time, then hot water is added thereto Prepared by dissolving the heat-treated dextrin and reacting with alpha-amylase and then decolorizing, desalting and drying through filtration and ion exchange resin after activated carbon treatment, or amyloglucosidase treatment, activated carbon treatment and glucose separation after alpha-amylase treatment. After the process, it was prepared by decolorizing, desalting and drying through activated carbon filtration and ion exchange resin. The indigestible dextrin prepared by the present invention possesses enzyme resistance to various enzymes, and it has been found to lower blood lipid concentrations, blood sugar and lipid content of liver tissues in rats, including drinks, ice cream, bread, confectionery, and the like. It can be widely used as a functional material in various foods. In addition, the indigestible dextrin prepared by the present invention is somewhat advantageous in terms of whiteness than the indigestible dextrin material made of underground starch, in particular, potato starch, it is expected that the field of food that can be applied is further expanded.

Description

Method for preparing indigestible dextrin with high enzyme resistance

FIG. 1 is a process diagram schematically showing a method for producing a high enzyme resistant indigestible dextrin of the present invention from heat treated dextrin.

[Purpose of invention]

[Field of the Invention and Prior Art in the Field]

The present invention relates to a method for preparing an indigestible dextrin having high enzyme resistance.

More specifically, the present invention relates to a method for preparing a high-enzymatic refractory dextrin by preparing heat-treated dextrin (pyrodextrin) using a starch material, followed by enzymatic treatment, filtration, decolorization, desalting and drying.

In general, plant fiber is defined as the total of indigestible components in plants that are not digested by human digestive enzymes. Plant fibers can be classified as water-soluble or insoluble depending on their solubility in water, and polydextrose is also included in plant fibers as synthetic plant fibers. Since these fibrils have a fibrous structure in which glucose and its derivatives or sugars other than glucose are combined, it has been observed that the structure is complicated and is difficult to digest (digestive) even when ingested in the body and is not ingested and released into the body. .

Indigestible substances, such as these plant fibers, exhibit various actions in the digestive tract and exert a physiological effect on the living body. For example, water-soluble plant fibers decrease the rate of movement of food and delay the absorption of nutrients in the upper digestive tract. The effect is such. The delay in absorption of nutrients has the effect of reducing the level of insulin in the blood by inhibiting the increase in blood glucose levels, and also lowering the cholesterol level in serum by promoting the secretion of bile acids. In addition, these indigestible substances are not digested and absorbed in the small intestine, but reach the large intestine and are partially degraded by the intestinal bacteria to produce short chain fatty acids. Acidification of the intestinal environment by short-chain fatty acids has the effect of intestinal action and colon cancer prevention, and the absorbed short-chain fatty acids are metabolized and converted into energy and inhibit cholesterol synthesis.

In recent years, as the standard of living improves, dietary life is changing in the West, and consumption of processed foods, cooked foods, fast foods, etc. is rapidly increasing, and the intake of fiber is decreasing. Fibrin deficiency has been suggested in various studies as one of the causes of adult diseases, and the necessity of fibrin has attracted new attention. On the other hand, consumers' desires are changing from nutrient-filled diets to health-oriented diets aimed at preventing nutritional disorders and various adult diseases caused by food habits, and among them, the demand for low-calorie foods is strong among adults, especially young women. Appearing. Thus, plant fiber is widely used as one of the ingredients to increase the functionality of food, such as constipation improvement and calorie reduction.

Water-soluble plant fibers among the plant fibers have attracted attention as a functional food material because they have a strong physiological action as described above, and are easier to process than insoluble plant fibers. These water-soluble plant fibers include natural gums such as guar gum, glucomannan, and pectin, but most of them are highly viscous and difficult to consume in large quantities alone. In addition, there are many difficulties in terms of processing to add to the processed food, there may be a problem in food manufacturing.

In the case of starch materials, alpha-starch, starch derivatives, glucose, starch derivatives, maltodextrin, etc., which are processed products of starch, are used in various processed foods as food materials, but most of these starch products have an indigestible component content of 5 It is less than% and contains a calorie value of 3.9 kcal / g or more, and what can be expected as a low-calorie food material that provides plant fiber in the starch system is limited to heat-treated dextrin.

In general, the heat treatment dextrin is prepared by high temperature heat treatment of starch containing a certain proportion of water in the presence or absence of acid. At this time, the starch mixture has a water-soluble complex structure containing a certain amount of non-digestible portion due to hydrolysis and random repolymerization, and the basic constituent glucose contains trace amounts of 1 → 3, 1 → 2 glycoside bonds, 1 → 4 and 1 → 6 glycosidic linkages (glycosidiclinkge) form.

There have been several proposals for the preparation of indigestible dextrins using starch materials, including:

EP 368451B1 discloses a process for preparing dextrins containing indigestible dextrins by dissolving the roasted dextrin in water and applying alpha-amylase, and applying alpha-amylase and adding hydrogen, alpha-amylase to the roasted dextrins. To be prepared by interaction with a glucosidase alone and co-working with beta-amylase, alpha-amylase and glucoamylase, followed by filtration, deodorization and desalting, followed by use of a strongly acidic cation exchange resin. A method for collecting plant fiber powder by chromatography and a method for producing plant fiber by mixing and roasting starch alone or monosaccharides and oligosaccharides with starch before preparing roasted dextrin are disclosed.

In addition, EP 477089A1 roasts potato starch in the presence of hydrochloric acid, and then reacts with alpha-amylase, reacts with glucoamylase, and then filters and removes plant fiber powder from the reactants by treatment with ion exchange resin chromatography or organic solvents. Disclosed is a method of manufacturing.

EP 487187A1 and US Pat. No. 5,430,141 disclose a method for producing low calorie foods, comprising low calorie maltodextrin obtained by adding inorganic acid to potato starch, dissolving the heated dextrin in water and applying alpha-amylase.

USP 5,264,568 adjusts the aqueous solution of the prepared Basodextrin to pH 7.0 to 8.5, pre-hydrolyze the solution by adding alpha-amylase derived from B. licheniformis, and then pH 5.5. To 6.5, hydrolyze again by raising the temperature, autoclave at a temperature above the boiling point of the solution, cool and add the alpha-amylase again, and autoclave again at a temperature above the boiling point of the hydrolysis solution. Disclosed is a method for preparing a hydrolyzate of basodextrin.

US Pat. No. 5,358,729 discloses a method for producing an indigestible dextrin with less coloring matter and less irritant odor, with hydrochloric acid added to starch and heated to 120-200 ° C. using an extruder, with a content of indigestion greater than 60%.

In EP 535627A1, EP 535627A1 and USP 5,364,652, hydrochloric acid added to starch is hydrolyzed with alpha-amylase and glucoamylase, and at least half of the resulting glucose is separated and removed, thereby reducing the content of the pyrolytic component. A method for producing a novel indigestible dextrin with less than 75% of the coloring matter and irritant odor is disclosed.

However, the above-described methods of the prior art have not only caused the reaction not to occur uniformly, but also have a problem that the discoloration of the starch causes the commercialization of the manufactured plant fiber, and the content of the indigestible component is low. .

Accordingly, the present inventors have improved the problems of the above-described prior arts in the application entitled "Method for producing plant fiber having a high content of whiteness and indigestible components" as follows (see Korean Patent Application No. 96-96). 1586):

In EP 368451B1, the starch is sprayed with liquid hydrochloric acid, followed by predrying and roasting the mixture to cause discoloration of the starch, whereas the inventors put corn starch in a dryer and inject hydrochloric acid in the form of a gas with hot air. The heat-treated dextrin prepared while preventing discoloration of the starch to the maximum was used for the production of plant fibers. In addition, in the prior art, monosaccharides and oligosaccharides were mixed with starch prior to preparing the roasted dextrin, but the present inventors simply dry-mixed a predetermined amount of functional additives in a heat treated dextrin with a V-mixer or the like. After the enzymatic reaction was carried out.

In addition, EP 477089A1 is aged by spraying a certain amount of hydrochloric acid solution on potato starch, pre-dried by flash dryer, roasted, hydrolyzed with alpha-amylase, glycosylated with glucoamylase, and then adjusted to pH to control glucoamylase. Plant fibers were prepared by activating and passing alkali metal type strong acid cation exchange resin or vacuum drying the precipitate formed using ethanol. However, the present inventors use white dextrin reacted with high temperature hot air for enzymatic reaction in order to prevent uniform reaction and discoloration of starch as much as possible, and to increase the content of indigestible component of plant fiber after glucoamylase reaction A certain amount of glucosidase was added, and the content of the indigestible component could be further increased through process improvement such as using a separation resin having a uniform particle size to separate monosaccharides in the reaction solution.

In EP 487187A1 and USP 5,430,141, 1% hydrochloric acid solution was sprayed and mixed with starch, pre-dried and heated, followed by addition of alpha-amylase to the reaction, followed by decolorization filtration, and alpha-amylase and glucose to the aqueous solution of Basodextrin. After reacting amylase and filtering and purification, a low calorie dextrin was prepared by separating the digestible portion using an alkali metal ion exchange resin or ethanol. However, the present inventors use the white dextrin prepared by injecting gaseous hydrochloric acid into starch together with high temperature hot air in the starch for the uniformity of reaction and prevention of discoloration of starch. In order to further increase, an indigestible functional additive was simply mixed dry with white dextrin prior to the enzyme reaction, and various enzymes were reacted with the mixture, and then a separated resin column having a uniform particle size and an organic solvent were used again.

In addition, USP 5,264,568 sprayed hydrochloric acid solution on potato starch, aged, pre-dried and alpha-amylase in heated basodextrin, which was subjected to primary hydrolysis at low temperature for a long time, and then the reaction solution was placed in an autoclave. In order to increase the content of the indigestible component of plant fiber in the white dextrin prepared through the uniform reaction through hot air, the present inventors dry-treated various functional additives in a dry manner. After mixing and hydrolyzing with alpha-amylase and reacting with glucoamylase and transglucosidase, ion exchange resin and organic solvent of uniform particle size were used to remove monosaccharides.

In USP 5,358,729, corn starch was added to a ribbon mixer, sprayed with 1% hydrochloric acid solution, mixed, preliminarily dried with a flash dryer, and extruded at 120 to 200 ° C. using a twin extruder. The inventors reacted white dextrin prepared with high temperature hot air with various enzymes.

In EP 535627A1, EP 535627A1 and USP 5,364,652, starch is sprayed with hydrochloric acid solution and aged for about 8 hours. After glycation was lowered to stop the action of the enzyme, the glucose portion was separated by more than 50% using a strongly acidic cation exchange resin column. Therefore, the present inventors put gaseous hydrochloric acid into the starch together with a hot hot air in order to produce the indigestible component of the dextrin itself and prevent the discoloration of the starch, and also neutralize the dextrin in advance by the reaction pH of alpha-amylase. The step of adjusting was omitted. In addition, the heat-treated dextrin was mixed with an indigestible functional additive, treated with alpha-amylase, inactivated by lowering the pH, and then transglucosidase was added to activate the glucoamylase and increase the content of the indigestible component of the reaction solution. After further reaction, the activated carbon was purified and filtered to remove the digestible parts such as monosaccharides with an organic solvent including ion exchange resin and ethanol of uniform particle size.

In addition, the dextrin prepared through the general rotary roaster may take a significant overload in the purification process such as decolorization of the sugar solution after the reaction uniformity and starch-specific whiteness, but the heat treatment dextrin used in the present invention is a high temperature The desired purpose could be achieved by using hot air and reacting gaseous hydrochloric acid with starch to prevent discoloration of the starch by oxidation. In addition, in the last step in the preparation of dextrin, it was possible to advance the hydrolysis immediately without neutralizing the pH of the aqueous solution of dextrin by pre-neutralizing to the action pH level of alpha-amylase.

In spite of solving the problems exposed to the prior art by the present inventors, the purification, decolorization, desalting and drying process of dextrin produced by the action of alpha-amylase is not sophisticated enough to produce high quality indigestible dextrin. In addition, since the resistance to the hydrolytic enzyme of the indigestible component of the heat-treated dextrin itself is not so high, inefficiency such as mixing the functional additives to prepare the final product to supplement the indigestible component is still an obstacle to industrialization. come.

Basic Fundamentals of the Invention

Thus, the present inventors subdivided the conventional purification process of the indigestible dextrin, it was confirmed that it is possible to efficiently increase the content of the high-enzyme-resistant indigestible component by the enzyme treatment, filtration, decolorization, desalting and drying process, The present invention has been completed.

After all, the main object of the present invention is to provide a method for producing an indigestible dextrin having high enzyme resistance.

Another object of the present invention is to provide an enzyme-resistant indigestible dextrin produced by the electric method.

[Configuration and Function of Invention]

In the present invention, an indigestible dextrin was prepared by dextrin prepared by heat treatment at high temperature as a raw material of starch as a plant fiber beverage and various functional health food materials as a raw material by enzymatic treatment, filtration, decolorization, desalting and drying.

That is, after pre-drying the general starch with hot air, a certain amount of gaseous hydrochloric acid (anhydroushydrochloric acid) is reacted with starch particles with high temperature hot air uniformly and heat-treated for a certain time, then hot water is added to dissolve the heat-treated dextrin After acting on alpha-amylase, it is decolorized, desalted and dried through activated carbon treatment, filtration and ion exchange resin to prepare indigestible dextrin, or after treatment with amyloglucosidase, activated carbon treatment and glucose separation after alpha-amylase treatment. After passing through, decolorizing, desalting and drying through filtration and ion exchange resin, an indigestible dextrin was prepared.

In order to establish the optimum enzyme treatment conditions in the preparation of the above-described indigestible dextrins, the present inventors use a commercially available dextrin as the heat treatment dextrin, the concentration of the heat treatment dextrin, the amount of alpha-amylase used in the enzyme treatment, and the enzymatic reaction. The time was confirmed. As a result, it was confirmed that the optimum heat treatment dextrin concentration in the amylase treatment was 35 to 45% by weight with respect to water, the appropriate enzyme concentration was 0.05 to 0.5% by weight with respect to dextrin, and the proper reaction time was 100 to 150 minutes. The best quality indigestible dextrins could be prepared.

The starch used in the present invention is a commercially available general starch, and may use ground starch such as corn starch, waxy corn starch and wheat starch, and underground starch such as potato starch, sweet potato starch and tapioca starch. For example, relatively low-cost corn starch may be used. The quality level of raw starch is generally acceptable if it is on the market.

In the following, the method for producing the pyrogenic dextrin of the present invention will be described according to the process (see FIG. 1).

First Step: Preparation of Heat Treated Dextrin

General starch is placed in a container of a fluidized bed dryer and pre-dried while starch is fluidized for about 30 minutes at 80 to 120 ° C, preferably 95 to 100 ° C with hot air. Hydrochloric acid at a concentration of 36% diluted 50% with distilled water is uniformly sprayed by a top spray method with 0.1 to 1% by weight, preferably 0.5% by weight of starch dry matter, with hot air introduced. As a heat source, it heat-processes at 110-180 degreeC, Preferably it is 130-140 degreeC using steam, and makes it react for about 150 minutes.

Subsequently, when the process is confirmed, the reaction is cooled to room temperature in a dryer, and the pH is adjusted by spraying ammonia bicarbonate or anhydrous sodium bisulfite dissolved in a small amount of distilled water as a neutralizing agent in the same manner as hydrochloric acid and mixing in a dry state. Maintained at 5.5 to 6.5, and cooled for another 30 minutes to prepare a heat treated dextrin. At this time, the addition amount of the neutralizing agent is preferably 0.1 to 1.0% by weight relative to starch in the case of ammonium bicarbonate, 0.1 to 0.5% by weight in the case of anhydrous sodium bisulfite.

Table 1 summarizes the analysis method and analysis results of the heat-treated dextrin prepared according to the first step.

Table 1: Analysis method and result of heat treatment dextrin

Claims (18)

Dissolving the heat-treated dextrin in hot water to react heat-resistant alpha-amylase, treating activated carbon and heating to inactivate the electric enzyme; Filtering the electrolytic reaction solution and decolorizing it by anion exchange resin; And, A method for producing a high-enzymatic, indigestible dextrin comprising the step of desalting and drying by adding an ion exchange resin mixed with a cation and an anion to an electrobleached sugar solution. The method of claim 1, Heat-treated dextrin is prepared by pre-drying the raw material starch with hot air, adding gaseous hydrochloric acid and heat treatment, and then mixing the neutralizing agent in a dry state A method for preparing an indigestible dextrin having high enzyme resistance. The method of claim 2, The neutralizing agent is characterized in that the ammonium bicarbonate or sodium anhydrous bisulfite dissolved in distilled water A method for preparing an indigestible dextrin having high enzyme resistance. The method of claim 1, wherein the heat-treated dextrin is dissolved at a concentration of about 30 to 50% by weight with respect to hot water of 70 to 90 ℃. The method of claim 1, 0.05 to 0.5% by weight of the heat-resistant alpha-amylase heat-treated dextriny is added, and the reaction is performed at 80 to 100 ° C. for 100 to 150 minutes. A method for preparing an indigestible dextrin having high enzyme resistance. The method of claim 1, Activated carbon is characterized in that about 0.5 to 1.0% by weight relative to the heat treatment dextrin A method for preparing an indigestible dextrin having high enzyme resistance. The method of claim 1, The pH of the alpha-amylase reaction solution is adjusted to 3.0 or less, and heated to 90 ° C. or more for about 20 to 40 minutes to inactivate it. A method for preparing an indigestible dextrin having high enzyme resistance. The method of claim 1, Filtration is a method for producing a high enzyme-resistant indigestible dextrin, characterized in that the filter carried out by glass and membrane filter paper. The method of claim 1, The anion exchange resin is added to about 2 times the volume of the enzyme treatment solution solids, and stirred for 20 to 30 hours at 35 to 45 ℃ characterized in that the decolorization A method for preparing an indigestible dextrin having high enzyme resistance. The method of claim 1, The ion exchange resin mixed with cation and anion is an activated resin mixed so that the volume ratio of cation to anion is about 1: 2. A method for preparing an indigestible dextrin having high enzyme resistance. The method of claim 1, Ion exchange resin mixed with cation and anion is added to the enzyme treatment solution about 2 times by volume, and stirred at 35 to 45 ° C. for 20 to 30 hours to decolorize dextrin. A method for preparing an indigestible dextrin having high enzyme resistance. The method of claim 1, The desalted sugar solution is concentrated to a concentration of 35 to 45%, and then dried by a spray dryer. A method for preparing an indigestible dextrin having high enzyme resistance. The method of claim 1, A process for treating amyloglocosidase between the reaction of alpha-amylase and the process for treating activated carbon; And a glucose separation process between the activated carbon treatment and the filtration process. A method for preparing an indigestible dextrin having high enzyme resistance. The method of claim 13, Amyloglucosidase is added at 0.01 to 0.1% by weight relative to the heat-treated dextrin at a temperature of pH 4.0 to 5.0 and 50 to 60 ° C. for 40 to 55 hours. A method for preparing an indigestible dextrin having high enzyme resistance. The method of claim 13, Separation of glucose is carried out using UBK 530 separation resin or ethanol A method for preparing an indigestible dextrin having high enzyme resistance. The method of claim 15, The ethanol treatment process is characterized in that the ethanol is added 3 to 7 times by weight to the heat-treated dextrin sugar solution adjusted to 30% by weight, left at room temperature for 2 to 4 hours, and the resulting supernatant is separated from the precipitate and treated. A method for preparing an indigestible dextrin having high enzyme resistance. A high enzyme resistant indigestible dextrin prepared by the method of claim 1. The method of claim 17, The enzyme is at least one member selected from the group consisting of saliva alpha-amylase, pancreatic alpha-amylase, small intestine enzyme and amyloglucosidase A method for preparing an indigestible dextrin having high enzyme resistance.