KR102140863B1 - Method for Manufacturing Skin Moisturizing Composition Comprising of Carriers Having Multi-Layer Structure - Google Patents

Abstract

The present invention is a step of preparing a hydrogenated lecithin dispersion by stirring and dispersing while adding hydrogenated lecithin to purified water at 70-90°C, and injecting saturated fatty alcohol having 12-22 carbon atoms into the hydrogenated lecithin dispersion at 70-90°C. While stirring and dispersing to prepare a pre-emulsion base, while stirring and dispersing while adding the pre-emulsion base and water-soluble polypeptide to purified water at 70-90 ℃ to prepare a composition base for skin moisturizing and at 70-90 ℃ Skin moisturizing containing a carrier having a multi-layered globules, characterized in that it comprises a step of preparing a multi-layered globules for skin moisturizing consisting of a phospholipid double layer by stirring and dispersing while adding oil to the composition for skin moisturizing. The present invention relates to a method for preparing a composition, and the present invention provides a carrier having a multi-layered globule having a multi-layered structure in which a hydrophilic (Hydrophilic) layer and a hydrophilic (Hydrophobic) layer are alternately formed using a multi-layered globule (liposome structure) composed of a phospholipid double layer. Since the composition for moisturizing the skin containing is prepared, a flexible and stable liposome structure such as a water balloon, unlike other micelle structures, can flexibly pass through a narrow gap of the skin, so that the water-soluble polypeptide (especially egg H-polypeptide-64 (Aquaporin) has the effect of providing a composition for moisturizing the skin containing a carrier having a multi-layered globule capable of stably delivering, the carrier of the present invention has a hydrophilic (Hydrophilic) layer and a lipophilic ( Hydrophobic layer has a multi-layered structure of alternating phospholipid layer, so that the hydrophilic (Hydrophilic) layer and the hydrophilic (Hydrophobic) layer easily pass through various tissues of the alternating skin. Moisture Factor) and water-soluble or oil-soluble moisturizing film-forming agent, it has the effect of providing a composition for moisturizing skin containing a carrier having a multi-layered globule that can stably deliver each.

Description

Method for Manufacturing Skin Moisturizing Composition Comprising of Carriers Having Multi-Layer Structure

The present invention relates to a method for preparing a skin moisturizing composition containing a carrier having a multi-layered globule, and more specifically, to prepare a pre-emulsion base using hydrogenated lecithin and saturated fatty alcohol having 12 to 22 carbon atoms, to the pre-emulsion base. It is intended to be used for a composition for skin moisturizing containing a carrier having a multi-layered globule, by preparing a multi-layered globule for skin moisturizing consisting of a phospholipid double layer by stabilizing by introducing a water-soluble polypeptide.

Skin performs various functions that affect the metabolism of our body and protects internal organs and protects lives from external factors that break the body's homeostasis. The main functions of these skins are 1) maintaining the balance of water in the skin, 2) protecting our body with the outermost part of the body that protects the internal organs, and 3) barrier function that prevents the loss of water and heat. 4) It plays a role of self-regeneration that maintains homeostasis.

There are two layers in the skin that are important for maintaining the homeostasis of moisture.

The first is the stratum corneum of the skin. The stratum corneum is largely formed of a lamella layer structure between keratinocytes and keratinocytes, which acts as a barrier to prevent the outflow of moisture into the body and prevent harmful substances from entering the body, and delivers skin active substances from the stratum corneum to the dermis of the skin epidermis. It acts as an impeding barrier and plays a decisive role in remarkably lowering the permeability of skin active substances. Its composition consists of keratin 59%, natural moisturizing factor (NMF) 31-38%, and intercellular lipid 11%. In particular, intercellular lipids are composed of ceramide, cholesterol, and fatty acids.

The second is the granular layer of the skin epidermis. In the granular layer, substances such as glycerol, hyaluronic acid, and collagen exist, which serve to hold water out. In addition, when the skin lacks moisture, Filaggrin, a protein that plays an important role in the skin barrier present in the granular layer, is used to maintain the skin's water homeostasis. This protein uses free fatty acids in the skin when water in the skin is insufficient. It decomposes and maintains the homeostasis of moisture inside the skin.

The skin is made up of about 70% or more of moisture, and it is absolutely necessary to maintain adequate moisture. If the water retention function decreases, it appears in serious phenomena such as dry skin and wrinkles.

The most ideal system for moisturizing the skin is 1) supplying moisture from the inside of the skin (granule layer) to maintain the moisture homeostasis of the skin, 2) keeping the phospholipid layer present in the outer layer of the skin (stratum corneum) healthy 3) skin It can be said to be a three-dimensional moisturizing system that satisfies all of the series of steps of forming a skin moisturizing film so that moisture is not lost from the epidermis to the atmosphere.

However, polyhydric alcohols, water-soluble polymers, and organic acid salts are currently used as skin moisturizers. As a moisturizer, they are oily and contain moisture that absorbs moisture and occlusive ingradient that prevents evaporation of moisture on the skin's surface. A method of increasing moisture retention in the stratum corneum using a humectant has been widely used.

As the moisturizing component (humectant), there are substances such as glycerin, propylene glycol, 1,3-butylene glycol and polyethylene glycol as polyols. As an occlusive ingredient, lipid components such as ceramide, essential fatty acids, and lipid complexes are used. However, they have a shortcoming that the moisturizing effect is temporary, so that they cannot continuously maintain the skin moisturizing effect for a long time or difficult to apply to various formulations.

In addition, they lack the supply of moisture from the inside of the skin and are oily while delivering the moisture on the surface of the skin to prevent evaporation of the occluded component (occlusive ingradient) and moisture-moisturizing component (humectant) that absorb moisture into the skin. Due to this lack, there was a problem that the skin moisturizing effect was not efficient.

Republic of Korea Patent No. 10-1805395 discloses a cosmetic composition for moisturizing the skin. The cosmetic composition for moisturizing the skin is formed by mixing ceramide, glycerin and butylene glycol in a specific content ratio to effectively form a moisture protective film on the skin, It is an excellent effect on moisturizing, and the moisturizing effect is temporary, so it cannot continuously maintain the skin moisturizing effect for a long time, and the supply of moisture from inside the skin is insufficient, and occlusive ingradient and humectant There is still a problem that the skin moisturizing effect is not efficient due to lack of delivery to the inside of the skin.

Republic of Korea Patent Registration No. 10-1805395 (Date of Publication: January 10, 2018)

The present invention has been devised to solve the above problems, and the object of the present invention is to use a stable multi-layered globule (liposomal structure) composed of a phospholipid bilayer to form a layer of alternating hydrophilic (Hydrophilic) and lipophilic (Hydrophobic) layers. By preparing a composition for moisturizing the skin containing a carrier having a multi-layered globule structure, unlike other micelle structures, a flexible liposome structure such as a water balloon can flexibly pass through a narrow gap of the skin, so that the water-soluble poly to the granular layer of the skin An object of the present invention is to provide a method for preparing a skin moisturizing composition containing a carrier having a multi-layered globule capable of stably delivering a peptide.

Another object of the present invention is to use a multi-layered structure of a phospholipid double layer in which a hydrophilic (Hydrophilic) layer and a hydrophilic (Hydrophobic) layer are alternately formed, and a number of skins in which a Hydrophilic (Hydrophilic) layer and a Hydrophilic (Hydrophobic) layer alternately appear. It is an object of the present invention to provide a method for preparing a skin moisturizing composition containing a carrier having a multi-layered globule capable of stably delivering a water-soluble or oil-soluble moisturizing film forming agent to each layer of the skin while passing through the tissue. Is done.

Another object of the present invention is to provide a method for preparing a composition for moisturizing skin containing a carrier having a multi-layered globule capable of easily manufacturing a stable water-soluble polypeptide carrier without the need for complicated processes or expensive equipment. .

Another object of the present invention is not affected by the surrounding environment, such as temperature or humidity, does not destroy the multi-layered globules composed of a phospholipid double layer over time, the hydrophilic (Hydrophilic) layer and the lipophilic (Hydrophobic) layer appear alternately It is an object of the present invention to provide a method for preparing a composition for moisturizing skin containing a carrier having a stable multi-layered globule capable of maintaining a multi-layered globule while passing through various tissues of the skin.

In order to achieve the above object.

Method for preparing a skin moisturizing composition containing a carrier having a multi-layered globule according to the present invention is a step of preparing a hydrogenated lecithin dispersion by stirring and dispersing while adding hydrogenated lecithin to purified water at 70-90°C, 70-90°C To prepare a pre-emulsion base by stirring and dispersing saturated fatty alcohol having 12 to 22 carbon atoms in the hydrogenated lecithin dispersion, and stirring while adding the pre-emulsion base and water-soluble polypeptide to purified water at 70-90°C. Dispersing to prepare a skin moisturizing composition base and 70-90° C. while adding oil to the skin moisturizing composition base and stirring to disperse to prepare a multi-layered globule for skin moisturizing consisting of a phospholipid double layer. It is characterized by being.

The method for preparing a composition for moisturizing skin containing a carrier having a multi-layered globule according to the present invention has the following effects.

First, a skin moisturizing composition containing a carrier having a multi-layered globule composed of a multi-layered structure in which a hydrophilic layer and a hydrodrophobic layer are alternately produced using a multi-layered globule (liposomal structure) made of a phospholipid double layer is prepared. As a result, unlike other micelle structures, a flexible and stable liposome structure such as a water balloon is able to flexibly pass through a narrow gap of the skin, so that a water-soluble polypeptide (especially R-polypeptide-64 (Aquaporin) without damage to the carrier to the granular layer of the skin) )) has the effect of providing a composition for moisturizing the skin containing a carrier having a multi-layered globules capable of stably delivering.

Second, the present invention has a multi-layered structure of a phospholipid double layer in which the delivery system alternates between a hydrophilic (Hydrophilic) layer and a hydrophilic (Hydrophobic) layer, so that the hydrophilic (Hydrophilic) layer and the hydrophilic (Hydrophobic) layer alternately appear on the skin. The effect of providing a composition for skin moisturizing containing a carrier having a multi-layered globule capable of stably delivering a water-soluble natural moisture factor and a water-soluble or oil-soluble moisturizing film forming agent to each layer of the skin while easily passing through the tissue. Have

Third, in the present invention, it is possible to easily produce a skin moisturizing composition containing a carrier having a stable multi-layered globule composed of at least two or more phospholipid bilayers without the need for complicated processes or expensive equipment, and thus has an economical effect.

Fourth, the present invention is not affected by the surrounding environment such as temperature or humidity, and as time passes, the multi-layered globules composed of a phospholipid double layer are stably maintained, so that the hydrophilic (Hydrophilic) layer and the lipophilic (Hydrophobic) layer alternately appear on the skin. It has the effect of stably delivering water-soluble or oil-soluble natural moisture factor and oil-soluble moisturizing film forming agent to each layer of the skin while passing through various tissues.

Figure 1a is a schematic diagram 1 for the activation of the internal water supply system.
Figure 1b is a schematic diagram 2 of the process for the activation of the internal water supply system.
Figure 1c is a schematic diagram of the structure of the water channel (Aquaporin) in the internal water supply system (Aquaporin).
2A is a view showing a reversible micelle structure of a hydrogenated lecithin dispersion prepared by stirring and dispersing while adding hydrogenated lecithin to purified water at 70-90°C.
2B is a view showing a cross-sectional structure of a pre-emulsion base prepared by stirring and dispersing saturated fatty alcohol having 12 to 22 carbon atoms in a hydrogenated lecithin dispersion at 70-90°C.
Figure 2c is a view showing a cross-sectional structure of the composition base for skin moisturizing prepared by stirring and dispersing while adding a pre-emulsion base and a water-soluble polypeptide, a water-soluble substance, to purified water at 70-90°C.
Figure 2d is a view showing a cross-sectional structure of a multi-layered globules for skin moisturization made of a phospholipid double layer prepared by stirring and dispersing while adding oil to the skin moisturizing composition base.
Figure 3 is a photograph taken with a polarizing microscope at 400 times magnification composition for skin moisturizing containing a carrier having a multi-layered globules, the present invention.
FIG. 4 is a hydration degree of untreated skin (Control) containing a carrier having a multi-layered globule (Control) and skin-treated composition (VENNARC-001) containing a carrier having a multi-layered globule. It is an ATR-FTIR image showing (Hydration Level) as the peak area ratio of the water to Amide II cm ^-1 .
5 is a hydration degree of the skin-treated composition (VENNARC-001) for the skin-moisturizing composition containing the carrier having a multi-layered globule (Control) This chart compares (Hydration Level) over time.

The terms or words used in the present specification and claims are not to be construed as being limited to a conventional or lexical meaning, and the inventor can appropriately define the concept of terms to describe his or her invention in the best way. On the basis of this, it should be interpreted as meanings and concepts consistent with the technical spirit of the present invention. Therefore, the embodiments shown in the specification and drawings of the present invention are merely the most preferred embodiments of the present invention and do not represent all the technical spirit of the present invention, and are replaced at the time of filing the present invention. It should be understood that various equivalents and variations that can be made are possible or possible.

Hereinafter, prior to explaining the present invention with reference to the embodiments, it does not show, or does not show, a configuration that is not necessary to reveal the gist of the present invention, that is, a known configuration that can be added by those skilled in the art. It is revealed that it is not described as.

The ideal system for moisturizing the skin pursued in the present invention is 1) supplying moisture from the inside of the skin (granule layer) to maintain moisture homeostasis of the skin, and 2) keeping the phospholipid layer present in the outer layer of the skin (stratum corneum) healthy. It can be said to be a system that satisfies all of the series of steps of forming a skin moisturizing film so that moisture is not lost from the skin epidermis to the atmosphere.

The skin moisturizing system pursued in the present invention is as follows.

1) The Internal Aqua supply System by Aquaporin

In order to maintain the water homeostasis of the skin, when the amount of water in the skin is insufficient, the Aquaporin component, which is known to activate this Filaggrin, is delivered from the outside to the granular layer in the skin to activate the Filaggrin to activate the water supply system from inside the skin. By reducing the dryness of, it can solve the skin pulling phenomenon and the skin drying phenomenon that appear when the skin is dry. In the present invention, the water-soluble polypeptide (Aquaporin) component known to activate Filaggrin is stably transferred from the outside to the granular layer in the skin by using a multi-layered globule for skin moisturization composed of a phospholipid double layer to activate Filaggrin to supply moisture from inside the skin. Activate the system.

Figure 1a is a schematic diagram for the activation of the internal water supply system, Figure 1b is a schematic diagram for the process of activation of the internal water supply system, Figure 1c is a water passage (Water Channel) structure of the aquaporin (Aquaporin) in the internal water supply system It is a schematic diagram of.

According to the drawings above, when the skin is dried, the internal Aqua supply system by Aquaporin operates as follows to maintain the homeostasis for moisture.

Step1. Keratin is granulated in the cells of the epidermis.

Step2. At the same time, Filaggrin is produced, complexing with Keratin. This complex prevents Filaggrin from being destroyed. [Profilaggrin: Keratin-Filaggrin Complex]

Step3. When this complex moves from the granular layer to the stratum corneum, a specific enzyme destroys the binding inside the Keratin-Filaggrin complex.

Step4. In the stratum corneum, Keratin is aligned to Filaggrin corneocytes.

Step5. Filaggrin arranged in this way is decomposed into a natural moisturizing factor, Free Amino Acids by a specific enzyme.

Step6. This free amino acid flows into the TCA Cycle to make and supply enough water inside the skin cells. Here, when the skin's water homeostasis is restored, the process of Step1~Step6 stops and it no longer supplies moisture to the skin.

Step7. Sufficient water formed in the cells maintains homeostasis to the moisture of the skin as it moves to the stratum corneum through the minute water passage created by the peptide component called Aquaporin between the skin cell membranes.

In the past, it was thought that water would enter the skin cells by diffusion. However, several studies have confirmed that the rate of movement of water into cells is 10 to 100 times faster than the rate of diffusion. Because of this, many scientists expected there would be another form of unknown water transport. As a result, in 1997, it was confirmed that a specific protein existed that promotes the inflow of water, and this protein was named Aquaporin. Aquaporin (Aquaporin) is a protein that forms a pathway through which the water can move through the cell wall. It was confirmed through an electron microscope that the water transport between cells is moved through a pathway composed of four aquaporin proteins. It was confirmed that aquaporin forms a nano-sized pathway and has a selective permeability to only water molecules, and its transport ability can transport 3 billion water molecules per second per aquaporin pathway. Therefore, in order to activate the internal water supply system [the Internal Aqua supply System by Aquaporin], as in the present invention, a water-soluble polypeptide (Aquaporin) component known to activate Filaggrin is used as a multi-layered globule for moisturizing skin composed of a phospholipid bilayer. It reliably transfers from the outside to the granular layer in the skin, activating Filaggrin to activate the water supply system from inside the skin, and sufficient water formed in the cells is transported to the stratum corneum through the minute water passage made by Aquaporin between the skin cell membranes and skin Homeostasis for moisture is maintained.

2) The External Aqua Supply Method by NMF

Another way to keep the skin dry by supplying moisture to the skin is to maintain the skin's moisture by supplying a natural moisture factor to the skin's surface to saturate the moisture on the skin's surface. It is a way to let it. This is also a natural moisturizing factor that exists inside the skin, hyaluronic acid, amino acids, and collagen, etc., and ingredients that are blended with polymer ingredients or wax ingredients. It is a method to keep the skin dry for a long time by minimizing the loss of moisture contained in the natural moisturizing factor by not drying it. In this case, in the present invention, the natural moisturizing factor is efficiently transmitted from the outside into the skin using a multi-layered globule for moisturizing the skin composed of a phospholipid bilayer, thereby effectively and effectively Stable expression can be performed.

3) Water Loss Protection from the skin surface

The internal hydration method using Filaggrin and Aquaporin and the hydration method from the outside of the skin through natural moisturizing factors are also important for maintaining the moisture of the skin, but the fundamentally important thing is to fundamentally manage to prevent moisture loss in the skin. The cause of the skin's greatest loss of moisture into the air is the damage to the phospholipids that make up the skin's membrane. When the phospholipids surrounding the skin cells are damaged, the moisture present in the skin cells is taken out into the air and drying of the skin proceeds rapidly, which causes the skin to suffer from diseases such as atopy. Therefore, in order to safely and ultimately solve the homeostasis of moisture in the skin, a method of restoring or regenerating the phospholipid membrane of damaged skin cells is needed. In the present invention, it is possible to have the effect of restoring the damaged phospholipids by stably transferring the useful moisturizing film forming agent component to the skin from the outside using a multi-layered globule for skin moisturization composed of a phospholipid bilayer.

As described above, the moisturizing problem cannot be fundamentally solved by simply applying the cosmetic composition to the skin, and the actions of 1), 2), and 3) must be combined, but the problem of skin moisturizing can be solved fundamentally and efficiently. Therefore, there is a need for the presence of a multi-layered globule for moisturizing the skin with a phospholipid double layer in which the hydrophilic (Hydrophilic) layer and the hydrophilic (Hydrophobic) layer of the present invention alternate. In general, since water-soluble polypeptide (Aquaporin) has hydrophilic properties that are easily soluble in water, it cannot pass through lipids between the stratum corneum and the hydrophobic keratinocytes to the granular layer of the skin, and it can also damage damaged skin. Phospholipid components are needed to restore, but the phospholipid components have too high a molecular weight that they cannot be transferred into the skin. Therefore, since the water-in-oil type or the oil-in-water type emulsification method cannot be delivered to the inside of the skin, the problem of the skin moisturization is fundamentally solved only by using the multi-layer vesicle for skin moisturizing of the present invention, which is a special delivery vehicle. Could. At this time, the applied delivery vehicle (Delivery Vesicle) must be composed of at least two or more phospholipid bilayers in order to activate the hydration system in each layer of the skin, and the layer structure in which the hydrophilicity and the hydrophilicity (Hydrophilic-Hydrophobic Layer Structure) alternately appears. Must have.

Since human skin maintains the homeostasis of moisture, it plays the role of the skin, so maintaining homeostasis of moisture is a very important part. The present invention, 1) is delivered to the skin through a multi-layered carrier containing a water-soluble polypeptide, and activates Filaggrin, a protein that plays an important role in generating moisture inside the skin, thereby supplying moisture from inside the skin and 2) skin It is intended to provide a composition that provides a three-dimensional water supply system capable of maintaining moisture for a long time by supplying moisture to a product, and 3) reducing water loss.

The present invention applies an emulsification technique to create a multi-layered globule for skin moisturizing, consisting of a phospholipid double layer containing a water-soluble polypeptide to activate the skin's internal hydration system, and multi-layered globules that can control the skin's water supply and moisture loss It is based on providing a skin moisturizing composition containing a carrier having a.

The present invention basically has three functions.

1) The Internal Aqua supply System by Aquaporin

Using the emulsification technology to create a multi-layered globule for skin moisturization composed of a phospholipid bilayer, it transfers a representative aquaporin component of the water-soluble peptide component to the skin layer below the stratum corneum of the skin to activate the Filaggrin protein to supply moisture from inside the skin. We intend to provide emulsification technology that can revitalize the system. The aquaporin component is also referred to as R-polypeptide-64 (Aquaporin). The core of the present invention is an internal moisture supply system that activates the Filaggrin protein by stably delivering a water-soluble polypeptide (especially Aquaporin) to the skin layer below the stratum corneum of the skin using a composition for moisturizing skin containing a carrier having a multi-layered globule [the Internal Aqua supply System by Aquaporin]. 2) The External Aqua Supply Method by NMF and 3) Water Loss Protection from the skin surface, as a rule, for complex and three-dimensional skin moisturization It is included additionally and supplementally.

2) The External Aqua Supply Method by NMF

It is intended to provide an emulsifying technology for a skin moisturizing composition containing a carrier having a multi-layered globule that maintains the persistence of moisture for a long time by supplying hydration by supplying moisture to the skin surface and inside when applied to the skin. At this time, the water-soluble natural moisturizing factor (Hydrophilic) is used, so it is moisturized by stirring and dispersing it while adding the pre-emulsion base and water-soluble polypeptide to purified water at 70-90℃ in the present invention. The composition base for skin moisturizing including a water-soluble natural moisture factor may be prepared by being additionally added to the water-soluble peptide in the step of preparing the composition base for water. Examples of natural moisturizing factors include glycerin, panthenol (type D), betaine, sodium hyaluronate, amino acids, collagen, glycosaminoglycan, sodium chondroitin sulfate or mucopolysaccharide. , These may be used alone or in combination of one or more. In the present invention, the internal water supply system through the delivery of the water-soluble peptide (Aquaporin) component to the skin layer below the stratum corneum using the composition for moisturizing the skin containing a carrier having a multi-layered globule [the Internal Aqua supply System by Aquaporin ] In addition to the activation effect of the external water supply method [The External Aqua Supply Method by NMF] through the transfer of water-soluble natural moisturizing factor to the skin layer below the stratum corneum of the skin [The External Aqua Supply Method by NMF] A three-dimensional skin moisturizing system in which moisture was supplied could be started.

3) Water Loss Protection from the skin surface

In the three-dimensional skin moisturizing system in which moisture is supplied from inside and outside of the present invention, a composition for moisturizing the skin is further required to reduce moisture loss by applying a moisturizing film to prevent loss of moisture on the skin by applying to the skin surface. Do. In the present invention, the above-mentioned problem was solved by including a moisturizing film forming agent in a multi-layered globule for skin moisturizing composed of a phospholipid bilayer. The moisturizing film forming agent can be largely divided into a water-soluble (Hydrophilic) and oil-soluble (Hydrophobic) moisturizing agent, and the water-soluble moisturizing film-forming agent is dispersed by stirring while adding a pre-emulsion base and a water-soluble polypeptide to purified water at 70-90° C. in the present invention. A skin-moisturizing composition base including a water-soluble moisturizing film-forming agent may be prepared by being additionally added to the water-soluble peptide in the step of preparing the skin-moisturizing composition base. The oil-soluble moisturizing film-forming agent is additionally added to the oil in the step of preparing a multi-layered globule for skin moisturizing consisting of a phospholipid double layer by stirring and dispersing while adding oil to the composition base for skin moisturizing at 70-90° C. in the present invention. A skin moisturizing composition base including a forming agent may be prepared. Examples of the water-soluble moisturizing film forming agent include glycerase, sodium oliboyl glutamate or xanthan gum, and these may be used alone or in combination of one or more. Examples of oil-soluble moisturizing film-forming agents include shea butter, beads wax, vegetable waxes, mineral waxes, animal waxes, silicones, silicone gums, silicone emulsions, silicone elastomers or vegetable butters, etc. One or more may be used in combination. By adding a configuration to prevent the loss of water supplied as described above, in addition to supplying water from inside and outside of the skin, a three-dimensional skin moisturizing system to prevent loss of supplied water could be completed.

In order to improve the moisturization of the skin, a water-soluble polypeptide, a natural moisture factor, and a vehicle capable of delivering a moisturizing film-forming agent into the skin are required. In order to make this carrier, the most important part is surfactant. In the present invention, hydrogenated lecithin was used instead of lecithin because of the stability problem of the carrier, and the pre-emulsion base was used using hydrogenated lecithin and saturated fatty alcohol having 12 to 22 carbon atoms. It was prepared. As described above, the pre-mixing process for manufacturing the pre-emulsion base is a key process that lowers the high interfacial tension of hydrogenated lecithin to improve compatibility with other materials and improves the stability of the carrier. The composition of the pre-emulsion base in this process is 1) hydrogenated lecithin, 2) saturated fatty alcohol having 12 to 22 carbon atoms, 3) water as the basic skeleton, separately 1) ceramide, 2) sterol, 3) ethanol (Ethanol) ), 4) fatty acids, and the like.

As described above, the pre-mixing process of manufacturing a pre-emulsion base to lower the high interfacial tension of hydrogenated lecithin to make it compatible with other materials and to improve the stability of the carrier is purified water at 70-90℃. Preparing a hydrogenated lecithin dispersion by stirring and dispersing while adding hydrogenated lecithin to the pre-emulsion base by stirring and dispersing saturated fatty alcohol having 12 to 22 carbon atoms in the hydrogenated lecithin dispersion at 70-90°C. It consists of the steps of manufacturing. The pre-emulsion base and the water-soluble polypeptide are stirred and dispersed while being poured into purified water at 70-90° C. to prepare a skin moisturizing composition base. Thereafter, the mixture is stirred and dispersed while adding oil to the composition base for skin moisturizing at 70-90° C. to prepare a multi-layered globule for skin moisturizing consisting of a double layer of phospholipids.

2A is a view showing a reversible micelle structure of a hydrogenated lecithin dispersion prepared by stirring and dispersing while adding hydrogenated lecithin to purified water at 70-90°C.

According to FIG. 2A, after heating purified water to 70-90° C., while slowly adding hydrogenated lecithin, stirring was performed for 8-12 minutes (preferably for 10 minutes) at ACI MIXER (general mixing mixer) rotation speed of 800-1000 rpm. The hydrogenated lecithin dispersion prepared has a structure in which spherical micelles and platelet micelles are reversibly present. In the present invention, it is preferable to adjust the concentration of phospholipids so that the platelet micelles dominate.

2B is a view showing a cross-sectional structure of a pre-emulsion base prepared by stirring and dispersing saturated fatty alcohol having 12 to 22 carbon atoms in a hydrogenated lecithin dispersion at 70-90°C.

According to FIG. 2B, while slowly adding saturated fatty alcohol having 12-22 carbon atoms heated to 70-90° C. to the hydrogenated lecithin dispersion, ACI MIXER (general mixing mixer) rotation speed was 800-1000 rpm for 8-12 minutes (preferably). For 10 minutes) The pre-emulsion base prepared by stirring has a structure in which saturated fatty alcohol is interposed between hydrogenated lecithins of platelet micelles. As such, saturated fatty alcohols are interposed between hydrogenated lecithins of platelet micelles, thereby stabilizing the unstable platelet micelle structure.

Figure 2c is a view showing a cross-sectional structure of the composition base for skin moisturizing prepared by stirring and dispersing while adding a pre-emulsion base and a water-soluble polypeptide, a water-soluble substance, to purified water at 70-90°C.

According to Figure 2c, while slowly adding the pre-emulsion base heated to 70-90° C. and purified water-soluble polypeptide R-polypeptide-64 (Aquaporin) in purified water, ACI MIXER (general mixing mixer) rotational speed was 8 at 800-1000 rpm. The emulsion base prepared by stirring for -12 minutes (preferably for 10 minutes) has a multi-layered structure in which the platelet micelles are elongated by water-soluble polypeptides. Since the platelet micelle is elongated by a water-soluble polypeptide and has a multi-layered structure, it has the effect that the multi-layered globules can be easily formed. In some cases, a water-soluble natural moisture factor and a water-soluble moisturizing film forming agent may be additionally added to the water-soluble polypeptide.

Figure 2d is a view showing a cross-sectional structure of a multi-layered globules for skin moisturization made of a phospholipid double layer prepared by stirring and dispersing while adding oil to the skin moisturizing composition base.

According to FIG. 2D, while gradually adding oil heated to 70-90° C. in an emulsion base at 70-90° C., a Homo Mixer (mixer for vertical stirring) rotational speed of 3000-4000 rpm and a paddle mixer (left and right) Mixer for agitation) The multi-layered globules composed of a phospholipid bilayer prepared by stirring at a rotation speed of 40-60 rpm for 8-12 minutes (preferably for 10 minutes) are elongated by a water-soluble polypeptide and have a multi-layered structure. The plate-shaped micelles are closed with spherical globules while surrounding the oil, and the socks are connected to form a carrier having a spherical multi-layer structure. At this time, each of the plurality of phospholipid bilayers (phospholipid membranes I, II, and III) is closed with a spherical multi-layer globule for skin moisturization. In some cases, oil-soluble moisturizing film-forming agents may be additionally added to the oil.

At this time, the water-soluble polypeptide, water-soluble natural moisturizing factor, or water-soluble moisturizing film-forming agent is located in the aqueous phase portion between the phospholipid bilayers, and the oil or oil-soluble moisturizing film-forming agent is located in the useful phase portion in the phospholipid double-layer. As described above, the water-soluble polypeptide, the water-soluble natural moisture factor, or the moisturizing film-forming agent is stably present in each layer of the multi-layer structure, so that the water-soluble polypeptide, the water-soluble natural moisture factor (Natural Moisture) without damage to the delivery system to the skin. Factor) or a moisturizing film-forming agent.

If the temperature conditions specified in the present invention and the rotational speed conditions of the mixer, the homer mixer, and the paddle mixer are not satisfied, there is a problem that the multi-layered globules for skin moisturization made of a phospholipid double layer are not produced. In particular, while slowly adding the oil heated to 70-90°C to the skin moisturizing composition base at 70-90°C, Homo Mixer (mixer for vertical stirring) rotation speed 3000-4000rpm condition and paddle mixer (left and right) Mixer for stirring) If the conditions for stirring for 8-12 minutes (preferably for 10 minutes) at a rotation speed of 40-60 rpm are not satisfied, a multi-layer structure for moisturizing the skin may not be formed.

In the hydrogenation lecithin dispersion manufacturing step, the hydrogenated lecithin is first dissolved in ethanol, and then stirred and dispersed while adding the hydrogenated lecithin dissolved in ethanol to purified water at 55-70° C. to prepare a hydrogenated lecithin dispersion. By going through a pre-treatment process, the temperature of the step of preparing the hydrogenated lecithin dispersion can be lowered from 70-90°C to 55-70°C to prevent the possibility of hydrogenation lecithin denaturation at high temperatures.

In the step of preparing a pre-emulsion base by stirring and dispersing saturated fatty alcohols having 12 to 22 carbons at 70-90° C. into the hydrogenated lecithin dispersion, saturated fatty alcohols include ceramide, sterol, or saturated fatty acids having 12 to 22 carbons. Any one or more may be additionally added. In this case, the saturated fatty alcohol of 12 to 22 carbon atoms should be reduced by the amount of added ceramide, sterol or saturated fatty acid of 12 to 22 carbon atoms. However, if excessive addition of ceramide, sterol, or saturated fatty acid having 12 to 22 carbon atoms is stabilized, in addition to the stabilizing effect of the unstable platelet micelle structure, the sock end may be connected first to form a spherical shape before the platelet micelles form a multi-layer structure.

In the present invention, the pre-emulsion base may be prepared using 30-75 parts by weight of purified water, 10-30 parts by weight of hydrogenated lecithin and 15-40 parts by weight of saturated fatty alcohol having 12-22 carbon atoms. When the composition of the pre-emulsion base is out of the above, there is a possibility that the effect of stabilizing the unstable plate-like micelle structure by the saturated fatty alcohol intercalating between the hydrogenated lecithins of the platelet micelles is consequently, and as a result, the skin active material carrier having a multi-layer structure It is possible that is not manufactured.

In the present invention, the skin moisturizing composition base may be prepared using 30-75 parts by weight of purified water, 15-40 parts by weight of a pre-emulsion base and 10-30 parts by weight of a water-soluble polypeptide. When the composition of the skin moisturizing composition base shown above is out of the composition, the plate-shaped micelles having a multi-layered structure and long extension by the water-soluble polypeptide are not formed, so that the multi-layered globules for skin moisturization are not easily formed and consequently the multi-layered globules are formed. There is a possibility that the composition for moisturizing the skin containing the eggplant is not prepared.

In the present invention, the multi-layered globules for skin moisturizing made of a phospholipid bilayer can be prepared using 5-25 parts by weight of oil in 30-75 parts by weight of the composition base for skin moisturizing. When the composition of the above-mentioned phospholipid double layer layer for moisturizing the skin outside the composition of the multi-layered globules does not occur, the action of closing the spherical globules with the spherical structure of the plate-shaped micelles having a multi-layered structure does not occur. There is a possibility that the composition for moisturizing the multi-layered globules may not be produced.

In the present invention, 15-40 parts by weight of saturated fatty alcohol having 12 to 22 carbon atoms may consist of 10-30 parts by weight of saturated fatty alcohol having 12-20 carbon atoms and 5-10 parts by weight of behenyl alcohol. In this case, the reason why behenyl alcohol is added is to lower the viscosity of the skin moisturizing composition containing a carrier having a multi-layered globule having a multi-layered structure. If the behenyl alcohol is less than 5 parts by weight, the effect of reducing the viscosity is too small. If the behenyl alcohol exceeds 10 parts by weight, there is a problem that the viscosity is too low.

In the present invention, the reason for using saturated fatty alcohol having 12 to 22 carbon atoms as saturated fatty alcohol is that when the number of carbons constituting saturated fatty alcohol increases, the viscosity of the emulsified product increases. This is because the result of decreasing viscosity is shown. At this time, the viscosity of the emulsified product may be adjusted by further mixing saturated fatty alcohol having 22 carbons. In addition, saturated fatty alcohols having a carbon number of less than C12 have a short carbon chain, and thus do not form a double layer (phospholipid bilayer) structure, which is the basic structure of a vehicle, and the stability of the formed multi-layer vehicle (Vehicle), even if it is produced. There is this falling issue.

In some cases, saturated fatty acids having 12 to 22 carbons may be additionally included to solve the problem of increasing the viscosity of the emulsified product as the number of carbons constituting the saturated fatty alcohol increases. The reason the carbon number of the saturated fatty acid to be added is 12 to 22 carbon atoms is because the use of saturated fatty alcohols having 12 to 22 carbon atoms in the present invention can increase compatibility with each other.

1. Test Example 1: Pre-treatment process for manufacturing a pre-emulsion base (Pre-Mixing Process for making a multi-layer vehicle)

Phase Raw material Name Wt.% Remarks A Water 75.00~30.00 Solvent B Hydrogenated Lecithin 10.00~30.00 Emulsifying Agent C Cetyl Alcohol or
Stearyl Alcohol or
Cetostearyl Alcohol or 10.00~30.00 Emulsion Stabilizer D Behenyl Alcohol 5.00~10.00 Emulsion Stabilizer Sum 100.00

A pre-emulsion base was prepared in the composition range of Table 2 as a pre-treatment process for manufacturing a skin active material carrier having a multilayer structure.

The specific conditions of the pretreatment process for manufacturing the skin active material carrier having the multilayer structure are as follows.

Step 1: Add phase A to the kiln and warm it to 80 degrees.

Step 2: While maintaining 80°C, phase B is slowly added to phase A while stirring and dissolving. (Agitation condition: AGI MIXER 900 rpm (or 800 to 1000 rpm), 10 to 20 min)

Step 3: Put D phase in an auxiliary kiln containing C phase and dissolve while warming to 80 degrees. (Agitation condition: AGI MIXER 300rpm)

Step 4: Add phase C to phase A at 80°C and stir with AGI MIXER to mix. (Agitation condition: AGI MIXER 900 rpm (or 800 to 1000 rpm), 10 to 20 min)

Step 5: After stirring, the mixed A+B+C+D phase is cooled to 50 degrees, sealed in a reservoir and stored at room temperature.

For reference, at this time, the composition of the substance and the pre-treatment process can be modified and modified as described below according to the purpose of use on the skin, such as maintaining the stability of the skin moisturizing composition containing the carrier having the multi-layered globules or moisturizing and protecting the skin. Accordingly, the possible additional options in the pre-processing process for manufacturing a pre-emulsion base (pre-mixing process for making a multi-layer vehicle) are as follows.

Selection 1: In the process of injecting and dissolving the B phase into the A phase as in the step of preparing the saturated fatty alcohol dispersion of the present invention, the process of first dissolving the B phase in ethanol (Ethanol) having a purity of at least 95% and then adding it to the A phase is added. Can be.

Option 2: In Table 2, a pretreatment process may be performed by additionally adding a ceramide-based raw material. Saturated fatty alcohol should be subtracted when mixing in proportion to the content of added ceramides.

Option 3: In Table 2, the raw material of sterols in phase C may be additionally added in a range of 0.10 to 2.00% to carry out a pretreatment process. However, saturated fat alcohol should be subtracted when mixing in proportion to the content of added sterols.

Option 4: In Table 2, a certain amount of fatty acid is added to the C phase to strengthen the phospholipid membrane formed of a phospholipid bilayer of the skin-active substance carrier having a multi-layered structure, or to lower the viscosity of the finally produced functional cosmetic composition. At this time, the type of fatty acid that can be used is a saturated fatty acid having 12 to 22 carbon atoms. Among them, long-chain fatty acids and ultra-long-chain fatty acids are generally used, among which behenic acid is preferred, and behenic acid has a long chain that has different influences on the viscosity of the final functional cosmetic composition. It is relatively large compared to fatty acids or ultra-long chain fatty acids, so its content should be well controlled. However, saturated fat alcohol should be subtracted when mixing in proportion to the added fatty acid content.

The composition of the material in Table 2 consists of 1) purified water, 2) hydrogenated lecithin, 3) saturated fatty alcohol, where saturated fatty alcohol serves to reduce the role of the emulsifying stabilizer and the high interfacial tension of the hydrogenated lecithin. Hydrogenated lecithin, which has both amphiphilic and hydrophilic properties, acts as an emulsifier. And saturated fat alcohol used at this time can be used alone or in combination of two or more, and C12 ~ C22 saturated fat alcohol having carbon number as the main component, but if you want to lower the viscosity of the product made by emulsification, behenyl alcohol, C22 It can be used further together.

When preparing a composition for such a pre-emulsion base, in order to positively affect the physical properties of the skin-active substance transporter, the state of emulsifying particles, and the manufacturing process, 1) ceramide, 2) sterol, 3) fatty acid, 4) ethanol, etc. The composition may be changed by further adding. For example, in order to strengthen the phospholipid double layer (phospholipid membranes I, II, and III) of the multi-layered emulsified particles, any one or more of ceramide, sterol, or saturated fatty acid having 12 to 22 carbon atoms at 70-90° C. has 12 to 22 carbon atoms. It may be further added to the step of preparing a pre-emulsion base by stirring and dispersing while adding saturated fatty alcohol to the hydrogenated lecithin dispersion. Hydrogenated lecithin has a hydrophilic head and a lipophilic tail and is not highly soluble in water at room temperature, so the hydrogenated lecithin is first dissolved in ethanol and then dissolved in ethanol first in purified water heated to 55-70°C. Agitation and dispersion may be performed while adding lecithin.

In addition, attention should be paid to temperature control in a pre-mixing process for making a skin moisturizing composition containing a carrier having a multi-layered globule. When it is kept at 70-90℃ for a long time for a long time, hydrogenated lecithin hardens under the influence of temperature, and its function is significantly weakened, and the ability to produce closed vesicles, namely Vesicle, that is, liposomes decreases, and thus the skin active material transporter thus produced Since the problem of moisturizing performance of the composition for moisturizing the skin containing a carrier having a multi-layered globules due to the weakening of the stability of the skin, a pre-emulsion produced through a pre-mixing process for making a skin-active substance carrier It is recommended to cool the base as soon as possible and store and store it at room temperature.

In addition, hydrogenated lecithin has a hydrophilic head and a lipophilic tail, and solubility in water is not high at room temperature. 1) At 70-90° C., saturated fatty alcohol having 12 to 22 carbon atoms is added to the hydrogenated lecithin dispersion and stirred. There are methods of dispersing to prepare a pre-emulsion base, and 2) dissolving hydrogenated lecithin in ethanol, followed by stirring and dispersing while adding hydrogenated lecithin dissolved in ethanol to purified water heated to 55-70°C. As a result, both methods of dispersing hydrogenated lecithin in water by the above two methods are possible and there is no difference in the degree of dispersion or physical properties regardless of the method selected because it is a matter of shortening the dispersion process time and efficiency.

Ceramide and sterol are one of the lipid membrane components of the epidermal stratum corneum among skin cells, and are substances that protect moisture lost from the skin's surface and prevent the penetration of harmful substances from the outside. Ceramide structurally combines with water to act as a lipid layer barrier, defends the body from external environments and microorganisms, and physiologically has a function of regulating cell growth with a signaling system. The role of ceramide and sterol in the process of making a skin-active substance carrier having a multi-layered structure makes the particles of hydrogenated lecithin well arranged, and exists between the hydrogenated lecithin particles of a double layer structure composed of a phospholipid bilayer. It acts to improve the stability of the skin-active substance carrier having a multi-layered structure by making the film of the membrane more dense. As shown in Table 2, the pre-emulsion base obtained in the pretreatment process for making the skin active material carrier having a multi-layered structure ranges from 10.00 to 20.00% by weight in a functional cosmetic product containing a skin moisturizing composition containing a carrier having a multi-layered globule. It is preferred to use the content of.

2. Test Example 2: Skin moisturizing composition containing a carrier having a multi-layered globule composed of a phospholipid double layer prepared using a pre-emulsion base (Preparation of multi-layer vehicle for skin moisturizing using a pre-mixing process)

Phase Raw Materials Name Wt % / % Remark A Green tea 44.86 Pre-Emulsion Base 16.00 Xanthan gum, glycerin, panthenol (type D) 6.05 Natural Moisture Factor Shea Butter Glycerize, Sodium Oleboyl Glutamate 2.00 Natural Moisture Factor and
Moisturizing film forming agent R-polypeptide-64 (Aquaporin) 2.00 Water soluble polypeptide VENNARC-001 BIOACTIVES 10.00 Eoseongcho Extract 2.00 Disodium LED, Alginine (L-type) 0.15 Dipotassium glyceride, adenosine 0.24 Nylon-12 1.00 B Cetostearyl alcohol 2.00 Stearic Acid 0.80 Sunflower seed oil, European hazel seed oil 6.00 Coco Glycerized, White Lupine Seed Oil 2.50 Shea butter, beads wax 0.80 Moisturizing film forming agent Glyceryl stearate, cetearyl glucoside 1.50 C Carbomer 0.10 D Golden extract, peony root extract 2.00 Sum 100.00

[Production process of skin moisturizing composition comprising multi-layered globules for skin moisturizing]

Step 1. Add phase A to the main kiln, then dissolve while heating to 80°C while stirring. (Agitation condition: PADDLE MIXER 50rpm)

Step 2. Dissolve A phase by heating to 80℃ and disperse uniformly using a Paddle Mixer and a Homo Mixer. (Agitation condition: Paddle Mixer 50rpm, Homo Mixer 3,000rpm, 10min at 80℃)

Step 3. Put phase B into the auxiliary kiln and then dissolve while warming to 80℃. (Agitation condition: Dispersing Mixer 200rpm)

Step 4. After putting phase B into phase A, emulsification is performed while stirring under a constant temperature condition of 80°C. (Agitation condition: Paddle Mixer 50rpm, Homo Mixer 3,200rpm, 8min, at 80℃)

Process 5. Cool the contents of the kiln while deaeration to 45℃. (Agitation condition: Paddle Mixer 40rpm, Cooling up to 40℃ under vacuum)

Process 6. After adding phase C to the main kiln, stir the contents uniformly. (Agitation condition: Paddle Mixer 40rpm, Homo Mixer 2,200rpm, 4min)

Process 7. After D phase is added to the main kiln, the contents are stirred uniformly. (Agitation condition: Paddle Mixer 40rpm, Homo Mixer 2,200rpm, 4min)

Process 8. Secondary degassing while cooling the contents of the kiln to 35℃. (Agitation condition: Paddle Mixer 40rpm, Cooling up to 35C under vacuum)

Step 9. After the contents of the kiln are floated in a separate storage container, they are aged for 3 days at room temperature.

In particular, in step 2, the homomixer (Homo Mixer: mixer for up and down agitation) rotational speed of 3000 rpm and the paddle mixer (Paddle Mixer: mixer for left and right agitation) rotation speed of 50 rpm are not uniformly dispersed at a temperature of 80° C. for 10 minutes or In step 4, if the homomixer (Homo Mixer: mixer for up and down agitation) rotational speed of 3000 rpm and the paddle mixer (Paddle Mixer: mixer for left and right agitation) rotational speed of 50 rpm are not uniformly dispersed for 8 minutes at a temperature of 80°C A skin moisturizing composition containing a carrier having a multi-layered globule composed of a phospholipid bilayer may not be formed.

Figure 3 is a photograph taken with a polarizing microscope at 400 times magnification of the composition for skin moisturizing containing a carrier having a multi-layered globules of the present invention prepared by the above process conditions. According to FIG. 3, it can be confirmed that the composition for moisturizing skin containing a carrier having a multi-layered globule according to the present invention is a multi-layered globule for skin moisturizing having a multi-layered structure composed of a plurality of phospholipid bilayers.

FIG. 4 is a hydration degree of untreated skin (Control) containing a carrier having a multi-layered globule (Control) and skin-treated composition (VENNARC-001) containing a carrier having a multi-layered globule. It is an ATR-FTIR image showing (Hydration Level) as the peak area ratio of the water to Amide II cm ^-1 .

It was measured by comparing the hydration degree of the skin treated with the composition for skin moisturizing containing the carrier having the multi-layered globules prepared in the composition and process of Table 2 and the untreated skin. Hydration level of untreated skin (Control) containing a carrier with a multi-layered globule (Control) and hydration level (VENNARC-001) of skin treated with a composition with a multi-layered vesicle (VENNARC-001) In the ATR-FTIR image represented by the peak area ratio of the water to Amide II cm ^-1 , if there are many red colors, it means that the hydration level is higher. According to FIG. 4, the comparison shows that the hydration level inside the stratum corneum is large in the skin treated with the skin moisturizing composition (VENNARC-001). That is, according to FIG. 4, it is shown that the hydration level is higher in the skin treated with the skin moisturizing composition (VENNARC-001) than in the skin not treated with the skin moisturizing composition (Control).

Figure 5 is a composition for skin moisturizing containing a carrier having a multi-layered globules of the present invention untreated skin (Non-application) and skin hydrating composition containing a carrier having a multi-layered globules treated hydration degree of the treated skin (Application) This chart compares (Hydration Level) over time.

This includes the Internal Aqua supply System by Aquaporin, The External Aqua Supply Method by NMF, and 3) Water Loss Protection from the skin surface. For measuring whether the complex works smoothly, a composition for skin moisturizing containing a carrier having a multi-layered globule untreated skin (Non-application) and a composition for skin moisturizing containing a carrier having a multi-layered globules treated skin For (Application), the skin hydration power of a long time in an anterior foil using Corneometer was repeatedly measured and analyzed using ANOVA. As a result, the hydration level in the skin (Application) was measured for 1 hour, 3 hours, 6 hours and 24 hours after the skin moisturizing composition containing the carrier having the multi-layered globules was processed. The hydration level was statistically significantly higher when compared with the non-application skin and the baseline (p<0.001) of the skin moisturizing composition containing the delivery vehicle.

Through the above experimental results, the skin moisturizing composition containing a carrier having a multi-layered globule treated skin (Application) The skin moisturizing composition of the present invention provides a sufficient hydration effect for a long time in the epidermal layer under the stratum corneum, and these results Through the present invention, it can be seen that hydration of the skin is continuously active by supplying aquaporine to the granular layer of the epidermis.

Hereinafter, the skin-moisturizing composition containing a carrier having a multi-layered globule through 50 specimens of the untreated skin (Non-application) and the skin-moisturizing composition-treated skin containing a carrier having a multi-layered globule (Application) The amount of water loss was compared.

[Experimental Example] Comparison of moisture loss in skin through VapoMeter (TEWL) Value measurement [Water Loss Protection from the skin surface]

Group N (number of samples) Missing
(Sample loss) Median
(median) 25%
(Average loss up to the top 25%) 75%
(Loss average up to 75% below) Baseline (no composition treatment) 50 0.000 13.850 11.475 17.925 Week 1 50 0.000 14.700 11.925 19.650 Week 2 50 0.000 14.700 11.250 18.250 Week 4 50 0.000 12.150 10.300 16.600 Week 6 50 0.000 12.600 10.975 15.150

Table 3 shows a week after treatment of a skin moisturizing composition comprising a multi-layered globule for skin moisturizing and a baseline that is not treated with a skin moisturizing composition comprising a multi-layered globule for skin moisturizing consisting of a phospholipid bilayer. It is the result of repeated measurement and analysis (Friedman Repeated Measures Analysis of Variance on Ranks, P = <0.01) using the Friedman method for the water loss after 4 weeks and 6 weeks.

Baseline vs. P Significance Week 1 0.313 No Week 2 0.521 No Week 4 0.035 No Week 6 0.005 Yes

Table 2 is a group treated with a skin moisturizing composition comprising a multi-layer globule for skin moisturization and a water loss amount of a baseline (control) that has not been treated with a skin moisturizing composition comprising a multi-layered globule for skin moisturization composed of a double layer of phospholipids. It shows the result of comparing the amount of water loss in (treatment group).(Multiple Comparisons: Wilcoxen Signed-Rank Test w/ Bonferroni-adjusted p-value of 0.013 (0.050/4))

In general, VapoMeter measures the rate of evaporation of water. The higher the water evaporation rate value, the greater the loss of water between the epidermis. The lower evaporation rate means that the moisture loss prevention effect is provided through the moisture film function and moisturizing function of the better epidermis. Compared to the baseline (control), the group treated with the skin moisturizing composition (treated group) steadily decreased the amount of water loss (TEWL) in the epidermis over time, and was statistically significant at week 6 It can be seen that indicates. In general, when the p value is 0.01 or less, the group (treatment group) treated with the skin moisturizing composition has a statistically significant effect of preventing water loss compared to the baseline (control) without treatment with the skin moisturizing composition. The group treated with the skin moisturizing composition (treated group) had a p=0.005 value at week 6 compared to the baseline (control group), which has a statistically significant reduction in the amount of water loss (TEWL) of the epidermis. I could confirm that there is. That is, it was confirmed that it has an excellent moisturizing effect when the skin moisturizing composition containing a delivery body having a multi-layered globule, which is the present invention, is treated.

Hereinafter, the composition of the skin cream, essence, mask pack liquid, and body cream is shown as an example of a cosmetic product prepared using a composition for moisturizing a skin containing a carrier having a multi-layered globule, which is the present invention.

Phase Raw Materials Name Wt.%/% Remarks A Purified water 40.06 Pre-Emulsion Base 18.00 Sodium hyaluronate (1% solution) 3.00 Natural moisturizing factor
(Natural Moisture Factor) Small sword 5.00 Moisturizing film forming agent R-polypeptide-64 3.00 Water soluble polypeptide Disodium ID 0.04 Allantoin 0.10 Arginine 0.20 glycerin 10.00 Natural moisturizing factor
(Natural Moisture Factor) B Cetyl alcohol 2.00 Stearic Acid 0.80 Jojoba oil, sunflower seed oil 12.00 Microcrystalline wax 0.80 Moisturizing film forming agent Jojoba butter 1.00 Moisturizing film forming agent Dimethicone 0.50 Moisturizing film forming agent Tocopheryl Acetate 0.20 Glyceryl stearate 1.00 C Carbomer 0.20 D Spices 0.10 E 1,2-hexanediol 2.00

Table 5 shows the composition of the skin cream, which is a cosmetic product prepared using a composition for moisturizing skin containing a multi-layered globule.

Phase Raw Materials Name Wt.%/% Remarks A Purified water 48.26 Pre-Emulsion Base 15.00 Betaine 3.00 Natural moisturizing factor
(Natural Moisture Factor) Small sword 5.00 Moisturizing film forming agent R-polypeptide-64 4.00 Water soluble polypeptide Disodium ID 0.04 Allantoin 0.10 Arginine 0.10 Glycosaminoglycan 10.00 Natural moisturizing factor
(Natural Moisture Factor) B Cetostearyl alcohol 1.00 Stearic Acid 0.50 Coco Glycerize, Sunflower Seed Oil 7.00 Beads wax 0.80 Moisturizing film forming agent Dimethicone 1.00 Moisturizing film forming agent Tocopheryl Acetate 0.50 Silicone gum 1.00 Moisturizing film forming agent Glyceryl stearate,
PAGE-100 Stearate 1.50 C Carbomer 0.10 D Spices 0.10 E 1,2-hexanediol 2.00

Table 6 shows the composition of the essence, which is a cosmetic product prepared using a composition for moisturizing skin containing a multi-layered globule.

Phase Raw Materials Name Wt. % / % Remarks A Purified water 64.79 Pre-Emulsion Base 10.00 Hydrolyzed collagen 3.00 Natural moisturizing factor
(Natural Moisture Factor) Small sword 5.00 Moisturizing film forming agent R-polypeptide-64 1.00 Water soluble polypeptide Disodium ID 0.04 Allantoin 0.10 Arginine 0.06 glycerin 5.00 Natural moisturizing factor
(Natural Moisture Factor) B Cetostearyl alcohol 0.70 Stearic Acid 0.30 Coco Glycerize, Sunflower Seed Oil 4.00 Mango seed butter 0.10 Moisturizing film forming agent Dimethicone 0.50 Moisturizing film forming agent Tocopheryl Acetate 0.10 Glyceryl stearate,
PAGE-100 Stearate 1.00 C Carbomer 0.06 D Spices 0.05 E 1,2-hexanediol 2.00

Table 7 shows the composition of a cosmetic mask pack liquid prepared using a skin moisturizing composition comprising a multi-layered globule.

Phase Raw Materials Name Wt. % / % Remarks A Purified water 52.31 Pre-Emulsion Base 12.00 glycerin 3.00 Natural moisturizing factor
(Natural Moisture Factor) Small sword 5.00 Moisturizing film forming agent R-polypeptide-64 2.00 Water soluble polypeptide Disodium ID 0.04 Allantoin 0.10 Arginine 0.10 Hydrolyzed collagen 2.00 Natural moisturizing factor
(Natural Moisture Factor) Sodium oliboyl glutamate 2.00 Moisturizing film forming agent B Cetostearyl alcohol 1.50 Stearic Acid 0.60 Olive oil, sunflower seed oil 10.00 Shea butter 3.00 Moisturizing film forming agent Dimethicone 0.50 Moisturizing film forming agent Tocopheryl Acetate 0.20 Glyceryl stearate,
PAGE-100 Stearate 1.50 C Carbomer 0.10 D Spices 0.05 E 1,2-hexanediol 2.00

Table 8 shows the composition of a body lotion that is a cosmetic product prepared using a skin moisturizing composition containing a multi-layered globule.

As described above, although the present invention has been described by limited embodiments, the present invention is not limited to the above embodiments, and those skilled in the art to which the present invention pertains may make various modifications and modifications from these descriptions. Deformation is possible. Accordingly, the spirit of the present invention should be understood only by the claims set forth below, and all equivalents or equivalent modifications thereof will be said to fall within the scope of the spirit of the present invention.

Claims (12)

Dissolving the hydrogenated lecithin in ethanol and then stirring and dispersing it while adding hydrogenated lecithin dissolved in ethanol to purified water at 55-70° C. to prepare a hydrogenated lecithin dispersion;
Preparing a pre-emulsion base by stirring and dispersing saturated fatty alcohol having 12 to 22 carbon atoms in the hydrogenated lecithin dispersion at 70-90° C.;
Preparing a composition base for skin moisturizing by stirring and dispersing while adding the pre-emulsion base and water-soluble polypeptide to purified water at 70-90°C; And
Preparing a multi-layered globule for skin moisturizing consisting of a phospholipid double layer by stirring and dispersing while adding oil to the skin moisturizing composition base at 70-90°C;
Method for producing a skin moisturizing composition containing a carrier having a multi-layered globules, characterized in that comprises a.
According to claim 1,
The water-soluble polypeptide is a H-polypeptide-64 (Aquaporin) method for producing a composition for moisturizing skin containing a carrier having a multi-layered globules.
According to claim 1,
The water-soluble polypeptide is a natural moisturizing factor (Natural Moisture Factor) and a water-soluble moisturizing film-forming agent is further added to the skin moisturizing composition containing a carrier having a multi-layered globules.
According to claim 1,
Method for producing a skin moisturizing composition containing a carrier having a multi-layered globules, characterized in that an oil-soluble moisturizing film-forming agent is additionally added to the oil.
According to claim 3,
The natural moisturizing factor (Natural Moisture Factor) is at least one of glycerin, panthenol (type D), betaine, sodium hyaluronate, amino acid, collagen, glycosaminoglycan, sodium chondroitin sulfate or mucopolysaccharide Method for producing a skin moisturizing composition containing a carrier having a multi-layered globules, characterized in that.
According to claim 3,
The water-soluble moisturizing film-forming agent is a method for producing a skin moisturizing composition containing a carrier having a multi-layered globule, characterized in that at least one of glycerase, sodium oliboyl glutamate or xanthan gum.
The method according to claim 1 or 2,
The step of manufacturing the multi-layered globules for skin moisturizing consisting of a phospholipid bilayer is a carrier having a multi-layered globules characterized in that it proceeds for 8-12 minutes under conditions of a homer mixer rotation speed of 3000-4000 rpm and a paddle mixer rotation speed of 40-60 rpm. Skin moisturizing composition manufacturing method comprising a.
The method of claim 7,
The step of preparing the hydrogenated lecithin dispersion proceeds for 8-12 minutes at a mixer rotation speed of 800-1000 rpm,
The step of manufacturing the pre-emulsion base proceeds for 8-12 minutes at a mixer rotation speed of 800-1000 rpm,
The step of preparing the skin moisturizing composition base is a method for preparing a skin moisturizing composition containing a carrier having a multi-layered globule, characterized in that the mixer rotates at 800-1000 rpm for 8-12 minutes.
delete The method according to claim 1 or 2,
Method for preparing a composition for moisturizing skin containing a carrier having a multi-layered globule, characterized in that at least one of ceramide, sterol or saturated fatty acid having 12 to 22 carbon atoms is additionally added to the saturated fatty alcohol.
The method according to claim 1 or 2,
The skin moisturizing composition is characterized by having one type of formulation selected from the group consisting of solution, suspension, emulsion, gel, lotion, essence, cream, powder, soap, pack, mask, cleansing, oil, foundation or spray. Method for producing a skin moisturizing composition containing a carrier having a multi-layered globules. According to claim 4,
The oil-soluble moisturizing film-forming agent is a multi-layered cow characterized by at least one of shea butter, beads wax, vegetable waxes, mineral waxes, animal waxes, silicone, silicone gum, silicone emulsions, silicone elastomers or vegetable butters. A method for preparing a skin moisturizing composition containing a delivery vehicle having a sphere.

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