KR102070316B1 - Composition for treating or preventing oral diseases comprising natural complex - Google Patents

Abstract

The present invention relates to a composition for oral cavity, and more particularly to a composition for preventing or treating oral disease. Clove extract of the present invention; And a composition of gallium extract, allium extract, self-designated extract and motherwort extract as an active ingredient at least one selected from the group is excellent in preventing or treating oral diseases. In addition, since the composition of the present invention is a substance derived from natural products, it is not toxic and does not cause side effects even when applied to the human body, and thus has high utility.

Description

Composition for treating or preventing oral diseases comprising natural complex}

The present invention relates to a composition for preventing and / or treating oral diseases.

As the concept of dental health is changed to the concept of social life, interest in oral health is increasing, and interest in bad breath, which is an important interpersonal disorder in social life, is increasing as well as prevention and treatment of oral diseases.

Dental tissue is composed of enamel, cement and dentin. The outside of the teeth exposed to the outside is covered with enamel, the outside of the root of the teeth located in the alveolar bone is covered with cement, dentin is present inside them. In dentin, microtubules called dentin tubules are distributed throughout the dentin.

Dental caries and periodontal disease are known to cause various clinical symptoms such as pain, masticatory dysfunction, periodontal tissue destruction, bad breath, and rash, and cause tooth loss. The causal factor is increasing.

Inhibits the growth of oral pathogens that cause dental caries, periodontal disease, bad breath, and syring, while living in the dental plaque, and various types of antimicrobial agents, including antibiotics, have bactericidal and bacteriostatic effects on these bacteria. It has been developed as an inhibitor and treatment for disease, pulp and root canal infection.

However, since antibiotics can cause the appearance and resistance to mycelial infection and systemic side effects with systemic side effects on our bodies, long-term use is difficult and can be used only as a therapeutic agent. In addition, antibacterial agents used in mouthwashes include Sangquinarine, Listerine, Peroxide, Chlorhexidine, etc. It is not clear about the treatment effect at the time, but it is also expensive. Listerine has alcohol as the main ingredient, but it has a temporary effect in the oral cavity but has a slight bacteriostatic effect. In addition, the peroxide that has been recently added for the whitening effect has a poisoning effect on bacteria, but at the same time has a poisoning effect on human tissues as well as a safety problem, and sometimes a bacterium resistant to peroxides appear in bacteria. In addition, chlorhexidine is known to be the best among the formulations known to date to prevent plaque formation and prevent and treat periodontal disease. However, chlorhexidine has a strong side effect on tissue irritation, staining and degeneration of tissues, especially irritating taste and odor. In addition, there are other disadvantages that can be caused by myocerosis, carcinogenicity is limited in the use of pregnant women and can not be used for a long time for the purpose of treatment or prevention.

Accordingly, the inventors of the present invention provide a natural material that is excellent in the prevention and / or treatment of oral diseases, that is, toothache, dental caries, gingivitis, periodontitis, bad breath, schizophrenia and the like without fear of such side effects. The study was conducted.

Republic of Korea Patent Publication No. 10-2015-0050995

The problem to be solved by the present invention is to provide a composition for the prevention and / or treatment of oral diseases, including as an active ingredient a component derived from natural products to ensure stability while showing an excellent effect on oral diseases.

In order to solve the above problems, the present invention is a clove extract; And a gallium extract, allium extract, self-designated extract and motherwort extract, any one or more selected from the group consisting of oral disease prevention and / or treatment composition, preferably oral composition, cosmetic composition, pharmaceutical composition or Provide a health food composition.

That is, the present invention is a clove extract; And it provides a new use for the prevention and / or treatment of oral diseases of any one or more extracts selected from the group consisting of gallium extract, alligator extract, self-designated extract and motherwort extract.

Clove (丁香, Syzygium aromaticum Merrill et Perry) is a part of the bud of the tropical evergreen subtilis clove tree. It is native to the Molucca Islands and is produced in Zanzibar Island of Tanzania, Sumatra in Indonesia, Brazil, Malaysia, the Philippines, Vietnam, and South China.

Gall flower is a legume, Pueraria. Dried flowers of lobata Ohwi, P. thunbergiana Bentham or P. hirsuta Matsum).

The allies are the Arctium , a compositae plant. The fruit of lappa Linne), also referred to as aksil (惡實).

The designation of the flower (紫花地丁) refers to the outpost of the violet ( Viola mandshurica W.Becker) or the violet ( Viola yedoensis Makino). Magnetization has a slight peculiar smell, is irritating, tastes very bitter, and is cold.

Chinese motherwort (말린 草) is the ground part of the flowering of Leonurus sibiricus L from the labiatae. Motherwort has a slightly peculiar smell, taste is bitter, very astringent, and slightly cold.

The present inventors have an excellent effect on oral diseases when using any one or more extracts selected from the group consisting of clove extract, wooja extract, self-specified extract and motherwort extract together with the clove extract, preferably clove extract, hwahwa extract, wooja extract It was found that the mixed extracts of the designated extracts, the designated magnetization extract and motherwort extract had the best synergistic biological activity.

In the present invention, the term "extract" refers to the extract itself, such as an extract obtained by the extraction process, a diluent or concentrate of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture thereof. And extracts of all formulations that can be formed using extracts.

The extract describes each plant and may include leaves, stems, bark, roots, flowers or flower buds, fruits, seeds, sap and whole plants in addition to the specified sites.

To prepare the extract, the skilled person can use any suitable method known in the art. For example, it may be by a vapor extraction method or a solvent extraction method. The whole or any part of the plant may be ground (eg a blender) and then the extraction solvent may be treated to yield a solvent extract. The grinding may be followed by a drying process (for example, drying for 15 to 50 hours at 40 to 70 ° C.) and then grinding. In addition, the solvent extract may be prepared in powder form by additional processes such as distillation under reduced pressure and lyophilization or spray drying.

The type of extraction solvent used is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of such extraction solvents include water; C1-C4 lower alcohols, such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether and dichloromethane; Or a mixture thereof can be used, Preferably, water and lower alcohol can be used individually or in mixture of 2 or more types. The solvent extract may be prepared by extracting one or more times using the solvent, and the dried extract obtained by freeze-drying or spray drying the solvent extract under reduced pressure may be prepared.

The amount of the extraction solvent may vary depending on the type of extraction solvent used, for example, it may be used in 1 to 20 times, or 5 to 20 times with respect to the dry weight of the target plant.

In addition, various extraction processes known in the art, for example, maceration, infusion, percolation, digestion, decoction, hot continuous extraction, aqueous-alcoholic extraction , Countercurrent extraction, microwave assisted extraction, ultrasonic extraction, supercritical fluid extraction, phytonic extract (e.g., hydro-fluoro-carbon solvent), etc. may be selected and used alone or It can be performed in combination of two or more methods.

The composition according to the present invention is a clove extract; And one or more selected from the group consisting of gallium extract, allium extract, magnetization designated extract and motherwort extract as an active ingredient, preferably clove extract, gallium extract, allium extract, magnetized extract and motherwort extract as an active ingredient Include.

In the present invention, "comprising as an active ingredient" means that the extract is added to the extent that can prevent oral disease and / or treatment effect from the composition according to the present invention, means that various components for stabilization, etc. It means that it can be added to the formulation in various forms (formulation).

The composition according to the present invention may comprise 0.00001 to 1% by weight of the active ingredient (clove extract; and at least one selected from the group consisting of gallium extract, allium extract, magnetized extract and motherwort extract) relative to the total weight of the composition, preferably Preferably 0.00005 to 0.5% by weight, and more preferably 0.0001 to 0.1% by weight.

In a preferred embodiment of the present invention, each plant extract in the composition according to the present invention comprising a clove extract, a gallbladder extract, a wooja extract, a designated magnetization extract and motherwort extract as an active ingredient may be included in the following ratio. For example, the weight ratio of the clove extract: browning extract: allium extract: magnetization designated extract: motherwort extract may be included as 1: 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10.

The composition according to the present invention may be for the prevention and / or treatment of oral diseases.

In the present invention, the oral disease is, for example, but not limited to, a comprehensive concept including toothache, dental caries, gingivitis, periodontitis, bad breath, shilin and the like.

In the present invention, the term "prevention" means any action that inhibits or delays a disease using the composition.

In addition, in the present invention, the term "treatment" means all the actions that alleviate or cure the symptoms of the disease using the composition.

In the present invention, the term "toothache" refers to pain that occurs in the tooth, and generally refers to pain that occurs in the tooth upon chewing. Causes of toothache include, but are not limited to, dental caries, pulpitis, ambush, tooth fracture, tooth abrasion, and the like. The inventors have found that the compositions according to the invention have excellent PGE2 inhibitory ability which can be an indicator of toothache.

In the present invention, the term "tooth caries" means that the tooth is damaged by bacteria in the oral cavity resulting in caries. For example, plaque, a biofilm produced on the tooth surface, may be the cause. The oral bacteria may be, but are not limited to, Streptococcus genus, Porphyromonas genus, and the like.

In the present invention, the terms "gingivitis" and "periodonitis", bacteria present in the subgingival plaque, for example, bacteria such as Streptococcus , Porphyromonas genus such that the appropriate growth conditions have been formed It is an oral disease that causes infection of the soft tissues of the gingival area and develops into periodontal disease of gingivitis or periodontitis and causes inflammation of the gums.

In the present invention, the term "bad breath" means that by the acquired systemic disease or protein in the saliva, food waste, etc. are generated by the decomposition of the substances produced by decomposition by microorganisms in the oral cavity to produce a substance causing odor do. What are the primary cause of oral microorganisms that cause bad breath are not limited to, Streptococcus (Streptococcus) genus Pseudomonas (Porphyromonas) in a foreskin, evil Tino Mai Seth (Actinomyces), An evil Tino Bacillus (Actinobacillus) in such This can be.

In the present invention, the term "single" means, but is not limited to, any phenomenon in which sharp, temporary or persistent pain appears with respect to external stimuli, independent of caries or other pathological causes. The external stimulus usually refers to a temperature stimulus, which usually causes symptoms at a cold temperature stimulus, which may cause pain at a hot temperature. In addition to such temperature stimulation, pain may also be caused by stimulation such as dry teeth, contact with foreign substances, and osmotic pressure through sweet or sour foods. It may appear throughout the entire tooth, or may be confined to the upper or lower jaw, or to specific areas such as the right or left side, and may be accompanied by dentin hyperesthesia, caries, and pulp inflammation.

The compositions of the present invention Streptococcus Pseudomonas (Porphyromonas) genus, and more particularly, Streptococcus mutans (streptococcus mutans), Streptococcus sanggwi switch (streptococcus sanguis), Streptococcus sanggwi varnish (Streptococcus sanguinis) to (streptococcus) in or wrapping , Streptococcus salivarius subsp. thermophils ), and Porphyromonas gingivalis ( Pirphyromonas gingivalis ) was confirmed that the excellent antibacterial effect on one or more bacteria selected from the group consisting of excellent effect in the prevention and treatment of oral diseases.

More preferably, the composition of the present invention is streptococcus mutans ( Streptococcus mutans ) or Porphyromonas jinjivalis ( Porphyromonas gingivalis ) It was experimentally confirmed that the antibacterial effect on bacteria is excellent.

The composition according to the present invention may be a composition for oral cavity.

In addition to the active ingredient, the composition for oral cavity may further include a wetting agent, an abrasive, a medicinal agent, a sweetener, a pH adjuster, a preservative, a binder, a flavoring agent, a foaming agent, etc., according to the formulation and the purpose of use.

The humectant may be used alone or in a mixture of concentrated glycerin, glycerin, sorbitol aqueous solution, amorphous sorbitol aqueous solution, polyethylene glycol, and propylene glycol, and the amount thereof may be included in an amount of 1 to 70% by weight based on the total weight of the composition for oral cavity. .

The abrasive is selected from the group consisting of precipitated silica, silica gel, zirconium silicate, calcium monohydrogen phosphate, calcium monohydrogen phosphate, hydrous alumina, hard calcium carbonate, heavy calcium carbonate, calcium pyrophosphate, insoluble metaphosphate and aluminum silicate. Etc. can be used. The amount of the abrasive may generally be included in an amount of 1 to 60% by weight based on the total weight of the composition for oral cavity. In addition, the additives used in small amounts may include pharmacological agents, sweeteners, pH adjusters, preservatives, colorants, foaming agents, binders and the like as conventionally used components, and may include 0.001 to 3% by weight relative to the total weight of the composition for oral cavity.

Pharmacologic agents include sodium fluoride, sodium monofluorophosphate, sodium fluoride, tin fluoride, chlorohexidine, allantoin chlorohydroxy aluminate, aminocaproic acid, zinc chloride, pyridoxine hydrochloride, tocopherol acetate, enzymes, etc. It can mix and use the above.

As the binder, carboxymethyl cellulose sodium, carbomer, carrageenan, xanthan gum, alginate and the like can be used.As a foaming agent, anionic and nonionic surfactants such as alkyl sodium sulfate, sodium lauryl sulfate, sucrose fatty acid ester and sorbitan fatty acid ester are used. It can be used individually or in mixture of 2 or more types.

Saccharin, xylitol, erythritol, aspartame and the like may be used as the sweetening agent.

Sodium phosphate, disodium phosphate, citric acid, triethanolamine, etc. can be used as a pH adjuster.

As the preservative, benzoic acid, methylparaben, propylparaben, sodium benzoate and the like can be used. As a fragrance, peppermint oil, spearmint oil, menthol, etc. may be mixed and used, and other additives, such as dexteranase, may also be used.

Examples of the foaming agent include sodium lauryl sulfate as anionic surfactant, a copolymer of polyoxyethylene polyoxypropylene as a nonionic surfactant (poloxamer), polyoxyethylene hardened castor oil, polyoxyethylene sorbitan fatty acid ester, and the like. .

In addition, it contains a residual amount of water.

The present invention also provides a product for oral cavity comprising the composition.

Clove extract; And oral extracts, alligator extracts, self-specified extracts, and motherwort extracts, including any one or more selected from the group consisting of an oral composition according to the present invention is generally included in all products manufactured and marketed for oral health. As an oral composition which can be used, there is no limitation on the oral product to be applied.

For example, the oral product may include, but is not limited to, toothpaste, oral spray, oral cleaner, oral ointment, oral cleanser, gargle product, gum, and the like. Oral products of the present invention may be in the form of liquid, solid, suspension, gel, aerosol, but is not limited thereto.

In addition, the composition according to the present invention may be a cosmetic composition.

The composition for the prevention and / or treatment of oral diseases is a solution, external ointment, cream, foam, nourishing longevity, softening longevity, pack, softening water, latex, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun May be prepared in a formulation selected from the group consisting of oils, suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays. However, the present invention is not limited thereto.

In addition, the cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and as conventional components, for example, oil, water, surfactants, moisturizers, lower alcohols, Thickeners, chelating agents, pigments, preservatives, flavoring agents, and the like may be appropriately blended, but are not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the dosage form. When the formulation of the present invention is an ointment, paste, cream or gel, the carrier component is animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide or Mixtures of these may be used.

If the formulation of the invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or mixtures thereof may be used, in particular in the case of a spray additionally chloro Propellants such as fluorohydrocarbons, propane / butane or dimethyl ether.

If the formulation of the invention is a solution or emulsion, a solvent, solubilizer, or emulsifier is used as the carrier component, for example water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3 Butylglycol oils, and in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, micro Crystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars and the like may be used as carrier components. Can be.

In addition, the composition according to the invention may be a pharmaceutical composition.

According to another embodiment of the present invention, the present invention is a clove extract; And it provides a pharmaceutical composition for oral disease prevention and / or treatment containing any one or more selected from the group consisting of gallium extract, wooja extract, self-designated extract and motherwort extract as an active ingredient.

Clove extract of the present invention; And a browning extract, a milky way extract, a designated magnetization extract, and a motherwort extract, the composition including any one or more may be various formulations of oral or parenteral. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants are usually used. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which form at least one excipient such as starch, calcium carbonate, sucrose or lactose (at least one compound). lactose) and gelatin. In addition to simple excipients, lubricants such as magnesium stearate, talc and the like are also used. Liquid preparations for oral administration include suspensions, solutions, emulsions, and syrups, and various excipients such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin, may be included. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used.

The invention also the clove extract; And it can be provided in the formulation of the skin external preparation for oral disease prevention and / or treatment comprising any one or more selected from the group consisting of gallium extract, wooja extract, self-designated extract and motherwort extract as an active ingredient.

The clove extract; And when using a composition comprising any one or more selected from the group consisting of gallium extract, allium extract, self-specified extract and motherwort extract as a skin external preparation, further comprising fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients , Antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, It may contain adjuvants commonly used in the field of dermatology, such as essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in external preparations for skin. In addition, the ingredients may be introduced in amounts generally used in the field of dermatology.

The clove extract; And if the one or more selected from the group consisting of gallium extract, allium extract, self-specified extract and motherwort extract is provided as a skin external preparation, but is not limited thereto, have a formulation such as ointment, patch, gel, cream or spray Can be.

The pharmaceutical composition of the present invention can be used as a parenteral preparation, for example, the external skin preparation may be a suitable pharmaceutically acceptable base such as petrolatum, stearyl alcohol, etc .; Pharmaceutically acceptable suitable surfactants such as polysorbate, sorbitan sesquioleate; Pharmaceutically acceptable humectants, such as glycerin; Pharmaceutically acceptable solvents; And it may be prepared by a conventional skin external preparation method of homogeneously mixing a flavoring agent, colorant, stabilizer, viscosity agent and the like.

The pharmaceutical composition of the present invention comprises an effective amount of the clove extract; And it may provide a desirable oral disease prevention and / or therapeutic effect when it comprises any one or more selected from the group consisting of gallium extract, ally extract, self-designated extract and motherwort extract. In the present invention, the term "effective amount" means an amount capable of sufficiently exhibiting oral disease prevention and / or treatment effects.

The clove extract included in the composition of the present invention; And at least one effective amount selected from the group consisting of gallium extract, alligator extract, designated magnetization extract, and motherwort extract will vary depending on the form in which the composition is commercialized, the method by which the compound is applied to the skin, and the time it stays on the skin. For example, when the composition is commercialized in medicine, the clove extract at a higher concentration than when commercialized in cosmetics that are routinely applied to the skin; And it may include any one or more selected from the group consisting of gallium extract, ally extract, magnetization designated extract and motherwort extract.

According to another embodiment of the present invention; And it provides a health food for oral disease prevention and / or treatment comprising any one or more selected from the group consisting of gallium extract, wooja extract, self-designated extract and motherwort extract as an active ingredient.

In the present specification, 'health food', clove extract; And one or more selected from the group consisting of gallium extract, allium extract, self-specified extract and motherwort extract, as an active ingredient to food materials such as beverages, teas, spices, gums, confectionery, or the like, encapsulated, powdered, suspensions, etc. As a manufactured food, it means that when ingested to bring a specific effect on health, unlike general medicine, there is no side effect that can occur when taking a long-term use of the drug as a food raw material. Since the health food of the present invention thus obtained can be consumed on a daily basis, an excellent effect can be expected for oral diseases, which is very useful.

The clove extract; And clove extract, when using any one or more selected from the group consisting of gallium extract, allium extract, self-designated extract and motherwort extract as an active ingredient, the clove extract; And one or more selected from the group consisting of gallium extract, alligator extract, designated magnetization extract, and motherwort extract, as an active ingredient, or may be used together with other food or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient can be suitably determined according to the purpose of use (prevention, health or therapeutic treatment). Generally, in the preparation of food or beverages, the composition of the present invention is added in an amount of up to 15 parts by weight, preferably up to 10 parts by weight based on the raw materials. However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety. . There is no particular limitation on the kind of the food. Examples of foods to which the substance may be added include meat, sausages, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, teas, drinks, Alcoholic beverages and vitamin complexes, and the like and include all of the health foods in the conventional sense. The health beverage composition of the present invention may contain various flavors or natural carbohydrates and the like as additional ingredients, as in the usual beverage. Natural carbohydrates described above are glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as tautin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like can be used. The proportion of said natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the composition of the present invention. In addition to the above, the health food of the present invention includes various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols. And carbonation agents used in carbonated beverages. In addition, the health food of the present invention may contain a flesh for preparing natural fruit juice, fruit juice beverage and vegetable beverage. These components can be used independently or in combination. The proportion of such additives is not critical but is usually selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Clove extract of the present invention; And a composition of gallium extract, allium extract, self-designated extract and motherwort extract as an active ingredient at least one selected from the group is excellent in preventing or treating oral diseases.

In addition, since the composition of the present invention is a substance derived from natural products, it is not toxic and does not cause side effects even when applied to the human body, and thus has high utility.

1 is a graph confirming the inhibitory effect of PGE2, a pain and inflammation marker of each extract ('extracts' are deleted from each plant extract and only the plant names are displayed).

Hereinafter, examples and the like will be described in detail to help the understanding of the present invention. However, embodiments according to the present invention can be modified in many different forms, the scope of the present invention should not be construed as limited to the following examples. Embodiments of the present invention are provided to more fully explain the present invention to those skilled in the art.

Material preparation

cloves  Preparation of Extract

Cloves were steam extracted to obtain a clove steam extract.

Gall painting  Preparation of Extract

Subcutaneous fragmentation was cut and immersed in a 95% ethanol solution, and extracted at room temperature for 48 hours to obtain a brown ethanol extract.

Allies  Preparation of Extract

Allies were chopped and immersed in a 95% ethanol solution and extracted at room temperature for 48 hours to obtain allium ethanol extract.

Preparation of magnetized extract

The magnetized directed ethanol was cut and immersed in a 95% ethanol solution and extracted at room temperature for 48 hours to obtain a magnetized directed ethanol extract.

Preparation of Motherwort Extract

Motherwort was chopped and immersed in 95% ethanol solution and extracted at room temperature for 48 hours to obtain motherwort ethanol extract.

cloves , Gall painting , Allies , Magnetization, motherwort ( 5 branches ) Preparation of Mixed Extract

Clove, browned, allium, magnetized, and motherwort extract prepared in the above-mentioned extract form in a ratio of 1: 1: 1: 1: 1 to prepare a clove, browned, allium, magnetized, and motherwort extract.

Example  And Comparative Example  Produce

The inventors prepared an example and a comparative example toothpaste for confirming the effect on oral diseases with the composition of Table 1 below. That is, to prepare toothpaste, sodium carboxymethylcellulose, sodium lauryl sulfate, glycerin (concentrated glycerin (98%)), sorbitol aqueous solution (70%), silica (precipitated silica), xanthan gum, sodium fluoride, pharmaceuticals, saccharin Comparative Example Toothpaste was prepared using citric acid, and the like, and an example toothpaste containing 0.01 wt% of a mixed extract was prepared.

Ingredient Name (Unit: Weight%) Example Comparative example Precipitated silica 20.0 20.0 Concentrated glycerin (98%) 30.0 30.0 Sorbitol aqueous solution (70%) 30.0 30.0 Sodium Lauryl Sulfate 1.0 1.0 Carboxymethyl Cellulose Sodium 0.5 0.5 Xanthan Gum 0.5 0.5 Sodium fluoride 0.22 0.22 Spices 1.0 1.0 saccharin 1.0 1.0 Citric Acid, Triethanolamine (TEA) Appropriate Appropriate Mixed extract 0.01 - Purified water To 100 To 100

< Experimental Example  1>

Confirm antimicrobial effect

In order to confirm the excellent antimicrobial effect of the mixed extract, the present inventors have shown that there is an effect of inhibiting the strain by using Gram-positive bacteria Streptococcus mutans and Gram-negative bacteria Popiromonas gingivalis, which are representative bacteria of dental caries and periodontal disease. Antimicrobial testing was conducted using a paper disk test. Each of the bacteria was grown in the optimum culture conditions of Table 2, and then cultured for about 4 to 6 hours in the optimum medium of each bacterium turbidity of the culture medium Macfarland turbidity No. After adjusting to 0.5 (1.5 × 10 ⁸ ), 0.1 ml were evenly spread. The sterilized paper disc (Whatman no.5 paper, 8 mm diameter) was inoculated at 10 mg / disc concentration for each fraction (Table 3) extract, and used after absorption drying for 1 hour. After culturing for 24 to 48 hours at the optimum temperature of each bacterium, the size of the growth resistant ring (diameter mm) was measured.

Strain Optimal condition Gram Dyeing
Gram staining Strain name Temperature badge characteristics Gram (+) Streptococcus mutans 37 ℃ BHI Facultative anaerobic Gram (-) Porphyromonas gingivalis 37 ℃ TSA Hemin Menadione medium Anaerobic

The results are shown in Table 3 (in the following section, 'extracts' are omitted from each plant extract).

division Growth ring ring diameter (mm) Streptococcus mutans Popiromonas Ginzy Balis No treatment -(No inhibition) -(No inhibition) 10 ppm cloves 11.3 11.0 Galvanized 10ppm 11.5 11.8 Magnetization specification 10ppm 11.5 11.3 Allies 10ppm 11.2 11.1 Motherwort 10ppm 11.1 10.9 5 ppm clove + 5 ppm browned 12.1 12.4 5 ppm clove + 5 ppm magnetization 12.1 12.0 5ppm Clove + 5ppm 12.0 12.1 5 ppm clove + 5 ppm motherwort 11.9 11.7 Clove 3.3ppm + Browning 3.3ppm + Magnetization 3.3ppm 12.5 12.8 Clove 3.3ppm + browning 3.3ppm + allies 3.3ppm 12.4 12.7 Clove 3.3ppm + browning 3.3ppm + motherwort 3.3ppm 12.3 12.9 Clove 3.3ppm + magnetization designation 3.3ppm + allies 3.3ppm 12.5 12.4 Clove 3.3ppm + Magnetization 3.3ppm + Motherwort 3.3ppm 12.5 12.1 Clove 3.3ppm + Allies 3.3ppm + Motherwort 3.3ppm 12.4 12.8 Clove 2.5ppm + browning 2.5ppm + magnetization designation 2.5ppm + allies 2.5ppm 12.7 12.4 Clove 2.5ppm + browning 2.5ppm + magnetization 2.5ppm + motherwort 2.5ppm 13.5 13.4 Clove 2.5ppm + magnetization designation 2.5ppm + allies 2.5ppm + motherwort 2.5ppm 13.0 13.1 2ppm Clove + 2ppm Galvanized + 2ppm magnetization + 2pp alligator + 2ppm motherwort 13.8 14.0

As a result, as can be seen in Table 3, it can be seen that the growth inhibitory ring diameter shows an excellent effect in the mixed extract containing the clove extract as compared to the respective extracts, clove, browning, magnetization, allergy and motherwort extract It was found that the growth-lowering ring diameters of all-containing ones were the widest for Streptococcus mutans and Popiromonas jinjivalis.

< Experimental Example  2>

PGE2  Confirm inhibitory effect

The present inventors confirmed the inhibitory effect of PGE2, a pain and inflammation marker, in order to confirm the toothache relieving effect of the mixed extract. Macrophages were inoculated at a concentration of 1.5 × 10 ⁵ cells / ml in DMEM medium containing 10% FBS and incubated in a 24 well plate at 37 ° C. and 5% CO ₂ for 24 hours. In addition, the inhibitory effect of PGE2 was evaluated in the supernatant cultured for 24 hours by treating LPS, which is an inflammation-induced stimulus, and each extract. The results are shown in FIG.

As a result, as can be seen in FIG. 1, compared to the respective extracts, it can be seen that the mixed extract containing the clove extract exhibits an excellent effect, and among them, the clove, the browning, the magnetization, the alligator and motherwort extract It turned out that the effect of containing is the best.

< Experimental Example  3>

Gingivitis  Confirmation of Formation Inhibitory Effect

The present inventors used the Example and Comparative Example toothpaste of Table 1 to confirm the inhibitory effect of gingival inflammation formation. Subjects were selected from 120 oral subjects with 30 oral examinations of 30 patients from ages 30 to 50 years old. A clinical trial was conducted on the effect of gingival inflammation.

The test subjects were divided into the experimental group (example) and the control group (comparative example), and then trained to use three times a day after sleep 2 hours after eating, and to perform an initial toothpaste to score an initial gingivitis index and to use each toothpaste composition. After 1 week, 1 month, 3 months and 6 months, oral examination was performed to check the gingivitis index. The gingivitis index is measured by inserting a periodontal probe into the gingival bulb and burning the probe around each tooth without applying force and measuring the bleeding state after 30 seconds. The score was recorded and the results obtained.

The results are shown in Table 5.

score Contents 0 points Bleeding 1 point Normal bleeding state 2 points Glandular bleeding 3 points Interdental triangle bleeding 4 points Gingival bleeding

term Control Experimental group Week 0 1.05 1.05 1 week 1.51 1.05 1 month 2.64 1.08 3 months 2.79 1.08 6 months 2.83 1.09

As a result, as can be seen in Table 5, in the experimental group using the example toothpaste was confirmed that the gingivitis index is almost not increased compared to the initial after 6 months, the control group using the comparative toothpaste It was confirmed that the rapid increase after one week did not inhibit the formation of gingival inflammation.

Therefore, it was confirmed that the composition according to the present invention has an excellent gingivitis inhibitory effect.

< Experimental Example  4>

Sirin  Confirm inhibitory effect

The present inventors used the Example 1 and Comparative Example toothpaste of Table 1 in order to confirm the inhibitory effect of aspirin. The subjects were 40 volunteers who had hypersensitive teeth and agreed to participate in this experiment. A total of 80 teeth were studied. In addition, there were 20 males and 20 females, and the age ranged from 20 to 50 years. The study subjects were not aware of the toothpaste contents and the total test period was 2 weeks (experimental group: Example, control group: comparative example).

The test was conducted by measuring the patient's response after applying the temperature stimulus. Prior to the test, each volunteer's hypersensitivity area was checked beforehand, and each of the compositions was used three times a day for two weeks after dropping the cold water of about 5 ° C with a dropper on the aerobic area of the tooth. After two weeks, the water was dropped again by dropping cold water at about 5 ° C. with a dropper. The response grade criteria of the patient after stimulation were set as follows. (0 points: no discomfort, 1 point: slightly uncomfortable or cold, 3 points: pain). Statistical analysis was performed using a paired student-t test for stimulation scores before and after 2 weeks in the laboratory.

Response scores for temperature stimulation after 2 weeks are shown in Table 6 below.

term Control Experimental group Week 0 2.14 ± 0.14 2.13 ± 0.20 2 weeks 2.15 ± 0.21 1.72 ± 0.09 p> 0.05 * p <0.05

As a result, as can be seen in Table 6, initially there was no significant difference in the response score for the temperature stimulation of the control group and the experimental group, but after 2 weeks, the control group did not have a difference, whereas the experimental group had a significant response score. Decreased.

In other words, using the Example toothpaste containing the mixed extract according to the present invention was confirmed excellent sirin inhibitory effect.

< Experimental Example  5>

Determination of bad breath

The present inventors used the Example 1 and Comparative Example toothpaste of Table 1 to confirm the bad breath removal effect.

Fifty men and women without dental caries were selected and subjected to a cross-over test. Commercial garlic powder was dispersed in water, left to stand for 24 hours, and diluted to make the Halimeter measurement value of 700 ppb or more, and the diluent was used as a source of bad breath. Gargle was diluted with 15 ml of garlic powder for 30 seconds, and after 1 minute, the degree of bad breath was measured with a halimeter, and then brushed with a toothpaste of Comparative Example (Control) and Example (Experimental Group) for 30 seconds to 1 minute. After 1 minute, 5 minutes, and 30 minutes after brushing the teeth, the degree of bad breath was measured by using a halimeter to determine whether the bad breath was continued.

The results are shown in Table 7 below.

term Control Experimental group 1 minute 55.8% 96.9% 5 minutes 45.1% 87.2% 30 minutes 37.2% 85.6%

As a result, it was confirmed that the experimental group using the example toothpaste as shown in Table 7 shows an excellent bad breath inhibitory effect compared to the control group using the comparative example toothpaste.

Claims (7)

Clove extract; And
Oral composition for preventing or improving at least one disease selected from the group consisting of one or more selected from the group consisting of gallium extract, wooja extract, self-specified extract and motherwort extract as an active ingredient, and sirini. delete The method of claim 1,
The active ingredient is a composition for oral cavity, characterized in that 0.00001 to 1% by weight based on the total weight of the composition. delete delete delete delete

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