JPS58172536A - Spectroscopic analysis apparatus - Google Patents
Spectroscopic analysis apparatusInfo
- Publication number
- JPS58172536A JPS58172536A JP5490782A JP5490782A JPS58172536A JP S58172536 A JPS58172536 A JP S58172536A JP 5490782 A JP5490782 A JP 5490782A JP 5490782 A JP5490782 A JP 5490782A JP S58172536 A JPS58172536 A JP S58172536A
- Authority
- JP
- Japan
- Prior art keywords
- light
- photodiode array
- spectroscopic analysis
- monochromatic
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
【発明の詳細な説明】 〔発明の技術分野〕 この発明は分光分析ｖｃ＠の技術分野に属する。[Detailed description of the invention] [Technical field of invention] This invention belongs to the technical field of spectroscopic analysis vc@.
従来、分析機器たとえば医療診断用の自動化学分析装置
に装備さｎている分光分析装置は、第１図に示すように
構成さｎている。ａ！１図に示す工うに、光源１からの
元を測定セル２内の測定試料に照射し、透過したｙｔ、
を大引スリッ）ＡＫ−介して回折格子４で分光し、分光
して得た各単色光ｔフォトダイオードアレイ６の受光素
子７で測定する工うになっている。BACKGROUND OF THE INVENTION Conventionally, a spectroscopic analyzer installed in an analytical instrument, such as an automatic chemical analyzer for medical diagnosis, is constructed as shown in FIG. a! In the process shown in Fig. 1, the light from the light source 1 is irradiated onto the measurement sample in the measurement cell 2, and the transmitted yt,
The monochromatic light beams are separated by a diffraction grating 4 through a large-scale AK beam, and each of the monochromatic lights obtained by the spectroscopy is measured by a light-receiving element 7 of a photodiode array 6.
しかしながら、従来の分光分析装置は、測定試料に直接
に元１１１からの元を照射しているので、測定試料中の
成分が尤による化学反応を起す。次とえば、測定試料が
高ビリルビン血清である場合に、尤に工りビリルビンが
分解してしまい、３００〜５００５ｍの波長領域での吸
光度値に誤差が生ずる。However, in the conventional spectrometer, the measurement sample is directly irradiated with the element 111, so that the components in the measurement sample undergo a chemical reaction. For example, when the measurement sample is serum with high bilirubin content, the bilirubin will be degraded and an error will occur in the absorbance value in the wavelength range of 300 to 5005 m.
分光分析によるデータ【医療診断の資料として提供する
自動化学分析装置Ｉにおいて、吸光度値が不正確という
Ｏは大きな問題点である〇
−万、近年の自動化学分析装置に対して、微量の測定試
料についてＯ正確な分析測定が要求さｎていて、前記分
光分析ｊｉｌｔでは必要とする測定試料の量が多過ぎる
という問題点％ある。Data from spectroscopic analysis [In automated chemical analyzers I that provide medical diagnostic materials, inaccurate absorbance values are a major problem. There is a problem in that an accurate analytical measurement is required for the spectroscopic analysis, and the amount of measurement sample required by the spectroscopic analysis tilt is too large.
この発８ＡＦｉ前記事情に鑑みてなさｎ次ものであｐ１
微量の測定試料を正確Ｋｖ＆元度分度分析ことのできる
分光分析装置を提供することｔ目的とするものである。This issue is 8AFi in view of the above circumstances and is the nth one p1
It is an object of the present invention to provide a spectroscopic analyzer capable of accurately performing Kv and element degree analysis of a trace amount of a measurement sample.
前記目的を達成するためのこの発明の概要は、光源から
の光【単色光に分光する分散素子と、分散素子で分光さ
ｎた単色光を受光し光電変換するフォトダイオードアレ
イと、フォトダイオードアレイの受光面前方に配置さｎ
ると共に測定試料を収容するセルとを具備することｔ％
像とする−のである。The outline of the present invention for achieving the above object is as follows: light from a light source [a dispersive element that splits into monochromatic light; a photodiode array that receives and photoelectrically converts the monochromatic light that has been split by the dispersive element; and a photodiode array. placed in front of the light receiving surface of
and a cell for accommodating the measurement sample.
It is an image.
ｉ２図にこの発明の一実施例を示す。 Figure i2 shows an embodiment of the present invention.
この発明の一実施例である分光分析ａｍは、フォトダイ
オードアレイ６の受光素子７の配ａｔｉｉｒ前方に、測
定試料液ｔ−流通させるための断面方形のｆＩｔ通セル
５を配置し、光源１ニジの元を入射スリン）３を介して
分散素子穴とえｉｊ回折格子４で分光し、分光した各単
色光を前記流通セル５に照射：１１：
し、流通セル５を透過し次元をフォトダイオードアレイ
６中の各受光素子７で關元する工うに構成さ扛ている。In the spectroscopic analysis am, which is an embodiment of the present invention, in front of the light receiving element 7 of the photodiode array 6, a fIt passage cell 5 with a rectangular cross section for flowing the measurement sample liquid T is arranged, and a light source 1 is connected to the light source 1. The source of the incident light is separated by the dispersion element hole and the ij diffraction grating 4 through the dispersion element hole and the ij diffraction grating 4, and each of the separated monochromatic lights is irradiated onto the circulation cell 5. Each light receiving element 7 in the array 6 is configured to operate as a base.
フォトダイオードアレイ６としては、通常のデュアルイ
ンピン型のものヲ使用すること−できるが、第６図に示
すものを使用すると、迷光を防止して正確な分光分析を
行なうことができる。第６図において、υで示すのはデ
ュアルインピン型のフォトダイオードプレイ６０ケース
であり、Ｕで示すのは受光素子７を配置Ｕするための凹
陥部であシ、ダで示すのは出力ビンであり、８で示すの
は保ＩＩｓ材である。保ｉｆＩ！Ｓ材８ｉ！、光透過帯
域が波長に工す相違する豪数のガラスフィルタ＆４次と
えはバンドパスフィルタ、／ヤーグカットフィルタを、
単色光Ｏ分散方向すなわち受光素子７の配残方向に沼っ
て、短波長歯から長政長肯へと順次に光透過帯域が変化
する工うに、配列してなる。このように、フォトダイオ
ードアレイ乙の保護部材８を複数のフィルタで構成して
おくと、流通セル５により生ずる迷光を除去することが
できるばかりか、従来のように保一部材がガラス板であ
った場合のガラス板に！る迷光を除去して、正確に吸光
度の測定を行なうことができる。As the photodiode array 6, an ordinary dual-in-pin type can be used, but if the one shown in FIG. 6 is used, stray light can be prevented and accurate spectroscopic analysis can be performed. In Fig. 6, υ is the dual-in-pin type photodiode play case 60, U is the recessed part for arranging the light receiving element 7, and DA is the output bin. The material shown by 8 is the Hou IIs material. Protect if I! S material 8i! , a large number of glass filters with different optical transmission bands depending on the wavelength, 4th-order bandpass filters, /Yag cut filters,
The monochromatic light beams are arranged in the dispersion direction, that is, in the direction in which the light-receiving element 7 is disposed, so that the light transmission band changes sequentially from the short wavelength band to the Nagamasa band. In this way, by configuring the protection member 8 of the photodiode array B with a plurality of filters, it is possible not only to remove stray light generated by the circulation cell 5, but also to eliminate the need for the protection member to be a glass plate as in the past. For glass plates in case! By removing stray light, absorbance can be measured accurately.
以上のＬうに分光分析装置を構成すると、流通セル５内
を通過する測定試料液ＫＦｉ、尤ｇ１１の元工りも弱め
らｎｅ単単色が照射さｎているので、元による化学反応
の起るお十扛はなく、吸光度値の誤差の発生を防止し、
正確な分光分析を行なうことができる。また、流通セル
５の断面積を小さくすると、微量の測定試料液について
の分光分析を行なうことができる。さらに、配置するフ
ォトダイオードアレイ乙により多数長分元分析を行な５
ことができる。When the spectroscopic analyzer is constructed as described above, the source of the measurement sample liquid KFi and G11 passing through the flow cell 5 is also weakened and is irradiated with a single monochromatic color, so that chemical reactions due to the source do not occur. There are no errors in the absorbance value, which prevents errors in absorbance values.
Accurate spectroscopic analysis can be performed. Further, by reducing the cross-sectional area of the flow cell 5, spectroscopic analysis can be performed on a minute amount of the measurement sample liquid. Furthermore, multiple long-element analyzes were performed using the photodiode arrays to be placed.
be able to.
以上、この発明の一実施例について説明したが、この発
明は前記実施例に限定さｎるものではなく、この発明の
要旨を変更しない範囲内で適宜に変形して、実施するこ
とができることはいうまでもないＯ
たとえば、他の実施例として１４図に示すように、受光
素子７ｆｔ配列し念受光面を２列に並べると共に、各受
尤面前方に２本の流通セル５Ａ、５Ｂｋ配置する分光分
析装置が挙げらｎる０この場合１本の流通セル５８には
対照液たとえば水ｔ−流通させ、他の１本の流通セルシ
には測定試料液を流通させる。この実施例によるとダブ
ルビームのリアルタイム多仮長ｆＲ元を行なうことがで
きる。Although one embodiment of the present invention has been described above, the present invention is not limited to the above-mentioned embodiment, and can be practiced with appropriate modifications within the scope of the gist of the invention. Needless to say, O For example, as shown in FIG. 14, as another example, 7 ft of light receiving elements are arranged, the optical receiving surfaces are arranged in two rows, and two circulation cells 5A and 5Bk are arranged in front of each receiving surface. In this case, a control liquid such as water is passed through one flow cell 58, and a measurement sample liquid is passed through the other flow cell 58. According to this embodiment, double-beam real-time polymorphic length fR elements can be performed.
ま念、流通セル５ （５Ａ、５Ｂ）は断面方形に限らず
、キャピラリー状にしてもよい。Please note that the flow cells 5 (5A, 5B) are not limited to having a rectangular cross section, but may have a capillary shape.
この発明によると、フォトダイオードアレイの前方に配
置する測定試料に単色光を照射するので、光源の尤を直
接に１１定試料に照射し急場合に比べて、測定試料の元
による化学変化を防止し、吸尤實値の誤差をなくすこと
ができる。シ九がって正確に分光外ＩｆＦｒを行なうこ
とができる。しかも、セルを細管状にすると微量の測定
試料の分光分析を行なうことができるっAccording to this invention, since the measurement sample placed in front of the photodiode array is irradiated with monochromatic light, chemical changes caused by the source of the measurement sample are prevented compared to cases where the light source directly irradiates the sample. Therefore, it is possible to eliminate errors in the absorption likelihood value. Therefore, extra-spectral IfFr can be performed accurately. Moreover, if the cell is made into a tubular shape, it is possible to perform spectroscopic analysis of minute amounts of measurement samples.
第１図は従来の分光分析装置を示す説明ス、第２図はこ
の発明の一実施例を示す説明図、第３図はフォトダイオ
ードアレイを示す断面図および第４図はこの発明の他の
実鳩ｃＡを示す説明図であろ０
１・・・光源、 ３・・・入射スリット、 ４・・・
回折格子、 ５・・・流通セル、 ６・・・フォト
ダイオードアレイ、 ７・・・受光素子。FIG. 1 is an explanatory diagram showing a conventional spectroscopic analyzer, FIG. 2 is an explanatory diagram showing an embodiment of the present invention, FIG. 3 is a sectional view showing a photodiode array, and FIG. 4 is an explanatory diagram showing an embodiment of the present invention. It is an explanatory diagram showing real pigeon cA0 1... light source, 3... entrance slit, 4...
Diffraction grating, 5... Distribution cell, 6... Photodiode array, 7... Light receiving element.
Claims (1)
素子で分光さｆＬ７を単色光を受光し光電変換するフォ
トダイオードアレイと、フォトダイオードアレイＯ受光
面前方に配置さｎると共に測定試料を収容するセルとを
具備すること倉特欧とする分光分析装置。A dispersive element that separates lt from a light source into monochromatic light, a photodiode array that receives the monochromatic light fL7 dispersed by the dispersive element and photoelectrically converts it, and a photodiode array arranged in front of the light-receiving surface and a measurement sample. A spectroscopic analysis device comprising a cell accommodating a cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5490782A JPS58172536A (en) | 1982-04-02 | 1982-04-02 | Spectroscopic analysis apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5490782A JPS58172536A (en) | 1982-04-02 | 1982-04-02 | Spectroscopic analysis apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS58172536A true JPS58172536A (en) | 1983-10-11 |
Family
ID=12983668
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5490782A Pending JPS58172536A (en) | 1982-04-02 | 1982-04-02 | Spectroscopic analysis apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS58172536A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4687329A (en) * | 1985-03-21 | 1987-08-18 | Abbott Laboratories | Spectrophotometer |
| JP2000321202A (en) * | 1999-05-10 | 2000-11-24 | Hitachi Ltd | Multi-wavelength spectrophotometer and photodiode arrayed photodetector |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54113382A (en) * | 1978-02-24 | 1979-09-04 | Toshiba Corp | Automatic analyzer |
-
1982
- 1982-04-02 JP JP5490782A patent/JPS58172536A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54113382A (en) * | 1978-02-24 | 1979-09-04 | Toshiba Corp | Automatic analyzer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4687329A (en) * | 1985-03-21 | 1987-08-18 | Abbott Laboratories | Spectrophotometer |
| JP2000321202A (en) * | 1999-05-10 | 2000-11-24 | Hitachi Ltd | Multi-wavelength spectrophotometer and photodiode arrayed photodetector |
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