JPS56129862A - Production of diagnosis latex reagent - Google Patents

Production of diagnosis latex reagent

Info

Publication number
JPS56129862A
JPS56129862A JP3316980A JP3316980A JPS56129862A JP S56129862 A JPS56129862 A JP S56129862A JP 3316980 A JP3316980 A JP 3316980A JP 3316980 A JP3316980 A JP 3316980A JP S56129862 A JPS56129862 A JP S56129862A
Authority
JP
Japan
Prior art keywords
latex
antibody
antigen
sensitized
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP3316980A
Other languages
Japanese (ja)
Other versions
JPS6116940B2 (en
Inventor
Toichi Yamada
Michio Kuge
Toshiaki Fukuda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sekisui Chemical Co Ltd
Original Assignee
Sekisui Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sekisui Chemical Co Ltd filed Critical Sekisui Chemical Co Ltd
Priority to JP3316980A priority Critical patent/JPS56129862A/en
Publication of JPS56129862A publication Critical patent/JPS56129862A/en
Publication of JPS6116940B2 publication Critical patent/JPS6116940B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PURPOSE:To achieve a highly sensitive and accurate diagnosis blocking nonpeculiar agglutination of a latex reagent by treating a serum containing an antibody after sensitization of the antibody to a latex particle. CONSTITUTION:An antibody is sensitized to a latex particle with a grain size of 0.01-1mu of a polystylene, stylene butadiene copolymer or the like in a weak alkali phosphoric acid buffer solution to obtain a sensitized latex removing unadsorbed antibody. The sensitized latex is incubated in a liquid in which the PBS is added to an anti-serum immunized by an antigen. The latex is centrifugally separated and redispersed in a PBS of about pH7 to obtain a latex reagent. This enables an accurate diagnosis without causing nonpeculiar agglutination tending to block reaction between an antigen and antibody in a serum being inspected. Thus, antigen on the surface of B hepatitis virus, for example, can be detected with a sensitivity about 1,000 times as large as that of the conventional reagent.
JP3316980A 1980-03-14 1980-03-14 Production of diagnosis latex reagent Granted JPS56129862A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3316980A JPS56129862A (en) 1980-03-14 1980-03-14 Production of diagnosis latex reagent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3316980A JPS56129862A (en) 1980-03-14 1980-03-14 Production of diagnosis latex reagent

Publications (2)

Publication Number Publication Date
JPS56129862A true JPS56129862A (en) 1981-10-12
JPS6116940B2 JPS6116940B2 (en) 1986-05-02

Family

ID=12379027

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3316980A Granted JPS56129862A (en) 1980-03-14 1980-03-14 Production of diagnosis latex reagent

Country Status (1)

Country Link
JP (1) JPS56129862A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995025958A1 (en) * 1994-03-24 1995-09-28 Kabushiki Kaisha Yakult Honsha Antibody-sensitized latex used for detecting nitrate or nitrite bacteria
EP0778466A1 (en) * 1995-06-19 1997-06-11 Kabushiki Kaisha Yakult Honsha Antibody-sensitized latex for denitrifying bacteria and method of detecting the bacteria
US5891382A (en) * 1988-09-26 1999-04-06 3D System, Inc. Recoating of stereolithographic layers
JP2006281196A (en) * 2005-03-11 2006-10-19 Toray Ind Inc Application apparatus, application method and method for manufacturing web having coating film
US8733275B2 (en) 2005-03-11 2014-05-27 Toray Industries, Inc. Application apparatus, application method and method for manufacturing web having coating film

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891382A (en) * 1988-09-26 1999-04-06 3D System, Inc. Recoating of stereolithographic layers
US6048487A (en) * 1988-09-26 2000-04-11 3D Systems, Inc. Recoating stereolithographic layers
WO1995025958A1 (en) * 1994-03-24 1995-09-28 Kabushiki Kaisha Yakult Honsha Antibody-sensitized latex used for detecting nitrate or nitrite bacteria
EP0778466A1 (en) * 1995-06-19 1997-06-11 Kabushiki Kaisha Yakult Honsha Antibody-sensitized latex for denitrifying bacteria and method of detecting the bacteria
EP0778466A4 (en) * 1995-06-19 1999-07-14 Yakult Honsha Kk Antibody-sensitized latex for denitrifying bacteria and method of detecting the bacteria
JP2006281196A (en) * 2005-03-11 2006-10-19 Toray Ind Inc Application apparatus, application method and method for manufacturing web having coating film
US8733275B2 (en) 2005-03-11 2014-05-27 Toray Industries, Inc. Application apparatus, application method and method for manufacturing web having coating film

Also Published As

Publication number Publication date
JPS6116940B2 (en) 1986-05-02

Similar Documents

Publication Publication Date Title
Coombs et al. On certain properties of antisera prepared against human serum and its various protein fractions; their use in the detection of sensitisation of human red cells with incomplete Rh antibody, and on the nature of this antibody
AU532303B2 (en) Determination of immuno-complexes in liquids
DK158284D0 (en) MONOCLONAL ANTIBODIES DERIVED FROM CROSS-BONDED FIBRIN DERIVATIVES AND ANALYSIS METHOD FOR DETERMINING THESE DERIVATIVES
GB1528071A (en) Radioimmunoassay
JPS55116258A (en) Microimmunoassay method
ES444543A1 (en) Immunological reagent and method of using same
NO153910C (en) DIRECT AGGLUTINATION TEST FOR DETECTION OF ANTIGENS IN BODY LIQUIDS.
ATE16219T1 (en) LATEX IMMUNOLOGICAL AGGLUTINATION PROCEDURE.
GR3005971T3 (en)
JPS56129862A (en) Production of diagnosis latex reagent
EP0106495A3 (en) Method and reagent for detecting antivirus antibody
DE3579856D1 (en) IMMUNULOGICAL TEST FOR DETERMINING COMMON, DETERMINING HEPATITIS B ANTIBODIES.
JPS5294418A (en) Diluent for measuring rubella hi antibody
JPS52125623A (en) Quantitative determination of antigenic substances and reagent kit for the same
JPS5759167A (en) Production of latex reagent
NO165128C (en) PROCEDURE FOR ELIMINATING FALSE POSITIVE RESULTS FROM SYSTEMIC LUPUSERYTHEMATODES PATIENTS IN DETERMINING AN ATL VIRUS ANTIBODY IN A TEST TEST.
JPS5757260A (en) Manufacture of latex reagent
EP0219279A3 (en) A reagent for detection and measurement of acetaldehyde-protein condensates in a fluid, together with its preparation and method of use
JPS5218815A (en) Novel method of immunological clinical diagnosis by means of gel-preci pitation
Rümke et al. How to enhance the sensitivity of the radial single diffusion technique for the quantitation of antigens
JPS5679956A (en) Erucinia enterocolitica polysaccharide sensitizing latex reagent and method of detecting erucinia enterocolitica polysaccharide antibody using this
HERRMANN et al. The problem of serological reactions for detecting foreign proteins in heated meat products
SE8101404L (en) REAGENTS AND SETS FOR DETERMINING ALDOLAS OF HUMAN MUSCLE TYPE
FR2274687A1 (en) Hepatitis antigen or antibody detection - using iodine 125-labelled antibody or antigen
SU1536315A1 (en) Method of revealing antibodies