JPH0892057A - Cosmetic blended with extract of seed of coffee tree - Google Patents

Abstract

PURPOSE: To obtain a cosmetic having stun-beautifying effect, preventing skin aging and protecting hair, comprising an extract prepared from seeds of a coffee tree containing chlorogenic acid as an essential component by using one or more solvents selected from among water, a lower alcohol and a polyol- based organic solvent. CONSTITUTION: An extract of seeds of a coffee tree containing chlorogenic acid as an essential component is found to have an inhibitory action on formation of lipid peroxides, an ultraviolet absorbing action and an inhibitory action on melanism and is applied as a cosmetic. The extract is useful in a field of a medicine/quasi-medicine and usable in a shape such as lotion, milky lotion, cream (including ointment), oil, pack, soap (including medicinal soap), body soap, bathing agent, shampoo, rinse, hair tonic or hair spray as a composition for various cosmetics in a field of cosmetic.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition containing a coffee tree seed extract containing chlorogenic acid as an essential component. It can be used as various cosmetic compositions in the fields of pharmaceuticals / quasi-drugs or cosmetics (various preparations used for humans and other animals). Specifically, lotions, emulsions, creams ( Applications include ointments), oils, packs, soaps (including medicated soaps), body soaps, bath agents, shampoos, rinses, hair nicks, hair sprays, etc.

[0002]

2. Description of the Related Art The skin and hair cover the surface of the living body and protect the living body from various factors such as bad environment such as ultraviolet rays, bacteria and dust. In other words, the skin and hair are those environments. Constantly exposed to the factors and hurt.

Therefore, research has been conducted for a long time for cosmetics and the like that protect skin and hair from environmental factors that have an adverse effect on the inside and outside of the body, and that promote regeneration of damaged skin and hair. Furthermore, in recent years, research aiming at beauty has been actively conducted, not only for the purpose of health, but also for the background of the psychology of always wanting to be beautiful.

[0004] In addition, many kinds of pharmaceuticals, quasi drugs, and cosmetics for the purpose of removing / eliminating factors adversely affecting the inside and outside of the living body are currently on the market, and among these effects / actions are: Lipid peroxide production inhibitory action, UV absorbing action,
The melanin production inhibitory action and the like are known.

[0005] For example, the lipid peroxide production inhibitory action is an action of preventing lipids (oils) existing in the living body from being oxidized. Lipid peroxide formed by oxidation of lipids impairs cell membrane function in the body, inactivates various enzymes, denatures proteins, etc., and adversely affects living tissues. These are also considered to lead to diseases such as aging and cancerous changes, and in recent years, suppression of this lipid peroxidation has been medically regarded as important.

[0006] Further, sunlight and ultraviolet rays are useful for humans in terms of health and hygiene such as synthesis of vitamin D, sterilizing and disinfecting action, but on the other hand, they cause sunburn, suntan, skin aging, and skin cancer. It also has the said harmful effects.

[0007] In a word, even if it is called ultraviolet rays, due to the difference in biological action, it is in the UV-C region of 290 nm or less, 2
90-320 nm UV-B region, 320-400 nm
It is divided into 3 regions of UV-A region, and UV-C region is an ultraviolet ray that has a strong effect on living organisms and is highly carcinogenic, but it does not reach the ground because it is almost absorbed by the ozone layer in the stratosphere. However, the decrease of the ozone layer has become a problem in recent years, and there is a concern that skin cancer will increase. UV rays in the UV-B and A areas also promote sunburn (acute inflammation), suntan (pigmentation), skin aging, skin cancer, etc., but when UV rays are applied to the skin, UV-
B penetrates the stratum corneum and epidermis, erythema appears after about 3 to 6 hours, and reaches a peak after 24 hours. Then, in 4 to 15 days, the erythema gradually shifts to blackening, which occurs because the melanocytes are activated by the inflammatory reaction by UV-B and the melanin production amount increases. In addition, keratinization progresses in the damaged epidermal tissue, causing rough skin due to a decrease in the water content of the stratum corneum.

[0008] Further, it is said that UV-A reaches the dermis layer, causes immediate blackening, and disappears in a short time. This is considered to be because existing melanin is temporarily oxidized and blackened. . With continuous exposure, delayed blackening occurs, and although erythema does not appear, melanocyte activation is remarkable and the degree of blackening becomes strong. Furthermore, since the skin permeability is high, the dermal tissue is severely damaged, the elasticity of the skin is lost, deep wrinkles are caused, and the color becomes dark brown. It is also necessary to protect the area, UV-A area).

Further, there are various enzymes involved in oxidation and reduction in the living body. Among them, as one of the causes of skin darkening, for example, tyrosinase enzyme is raised, and
Several metabolic mechanisms are considered in relation to the change (phenomenon) of pigments in plants. One of them is the activation of tyrosinase enzyme in melanocytes to oxidize tyrosine and convert it into dopaquinone. However, it is further known that it is oxidized to dopachrome, 5,6-dihydroxyindole, which is polymerized and finally produces black melanin. Therefore, there is a demand for a substance that prevents abnormal blackening of the skin based on such a biochemical mechanism. There is also a need for a substance that inhibits the activation of tyrosinase, which is also involved in the formation of melanin pigment.

On the other hand, chlorogenic acid is said to have been industrially isolated from green coffee beans for the first time, and it is known that Robusta seeds have a higher chlorogenic acid content than Arabica seeds. ing. In addition, chlorogenic acid is widely present in other plants and is contained in fruits of many dicotyledons, leaves, and examples of plants containing chlorogenic acid are tobacco leaves, pear leaves, apple pulp, sweet potato, mulberry,
Tea etc. are known.

Incidentally, a report on the application of chlorogenic acid to the industrial field has been recently made, and the publication is 1: JP-A-57-83250 (Sugar and starch syrup as a method for producing candy). Chlorogenic acid is added to the main ingredient candy to prevent browning of the candy),
2: JP-A-57-115147 (chlorogenic acid and other substances are added to foods and drinks containing saccharides to prevent browning due to caramelization of sugars).
17566 (Similarly, chlorogenic acid and other substances are added to the paprika pigment to prevent the fading of the paprika pigment over time) 4: JP-A-58-138347 (natural edible antioxidant substance of enzymatically decomposed coffee beans) 5: JP-A-60
-192555 (Anti-allergic food containing chlorogenic acid as an essential component), 6: JP-A-62-111671
(Natural anti-acid agent for hot water extract of green coffee beans), 7:
Table 63-502349 (Isolated chlorogenic acid is administered before or during the administration of food, beverage or drug that damages the mucous membrane of the stomach or duodenum or promotes gastric acid secretion,
Protecting gastric mucosa against progression of ulcer) 8: JP-A-02-
100600 (Mulberry / tea extract is added to food, this food is wrapped in wrapping paper having far-infrared radiation and ethylene gas adsorption, and stored at low temperature to maintain long-term freshness and browning of food surface. Prevention), 9: JP-A-04-
27374 (containing chlorogenic acid and vitamin C and the like in food and drink to suppress the reduction and change of aroma and flavor during the processing and storage of food and drink and the generation of off-flavors), 10: JP-A-04-04
-283501 (Cut flower freshness-retaining agent), 11: JP-A-06-06
-9603 (stabilization method of vitamin C) and the like have been reported.

[0012]

As described above, most of the utilization of chlorogenic acid is to prevent fading and browning of foods and drinks, to suppress off-flavors, to protect mucous membranes of stomach and duodenum, and vitamin C. It is intended to stabilize the drug, and the action and effect can be obtained by oral administration. On the other hand, in the field of cosmetics, none has been effectively used. Therefore, the present inventors have conducted intensive studies to study and pursue the unique novel and useful utilization of coffee tree seed extract containing a high amount of chlorogenic acid and its action.

[0013]

In view of the above circumstances, the present inventors have found that coffee seeds contain a large amount of chlorogenic acid. Therefore, a chlorogenic acid-containing extract obtained by extracting coffee coffee seeds is obtained. Consider things,
The present invention was completed by confirming that it has the above-mentioned lipid peroxide production inhibitory action, ultraviolet ray absorption action, and melanin production inhibitory action, and found that it is extremely effective to apply it to cosmetics. The process leading to the present invention will be described below.

The coffee tree of the present invention is an evergreen shrub native to the tropical African region of the Rubiaceae family, mainly in Ethiopia. The leaves are long-ovate, somewhat leathery, and bloom on year-round, and the flowers are clustered in axillary with a short floral pattern. To do. The base of the corolla is tubular,
The tip is divided into several pieces, with a white color and fragrance. The fruits are small spheres or ovals, initially green, reddish and purple with ripening and usually contain two seeds. This seed is hemispherical and has one deep groove on the flat surface, called flat bean,
Fruits are harvested and imported 8-9 months after flowering. still,
In the present invention, coffee arabica
L.) seeds are used, but in addition, coffee canephora Pier (Coffee canephora Pier)
r.) and Liberia coffee (Coffee liberica Bul)
It is also possible to use seeds of l.).

Further, extraction of coffee tree seeds from the seeds of coffee trees is carried out raw or after drying and crushing, and as a solvent, an organic solvent, water or hot water, an organic solvent or a mixed solvent with water, and further an organic solvent. A combination of solvent extraction and water extraction may be used. Further, as the organic solvent, methanol, ethanol,
N-butanol, acetone, chloroform, ethyl acetate, n-hexane, 1,3-butylene glycol, propylene glycol and the like are used.

The extraction conditions are not particularly limited, but usually room temperature and heat extraction are preferred. After extraction, the solution may be filtered, concentrated and dried to be used as a solution, paste or powder. In many cases, it can be used as it is, but if necessary, purification treatments such as deodorization and decolorization may be added within a range that does not affect its efficacy. As a purification treatment means for deodorization, decolorization, etc., an activated carbon column or the like may be used, and any ordinary means generally applied depending on the extraction substance may be arbitrarily selected.

Chlorogenic acid, which is an essential component of the present invention, includes mono- and dicaffeoylquinic acid (Caffeoylquinic acid).
acid) and mixtures thereof, with preference given to 3-, 4- and 5-caffeoylquinic acid or mixtures thereof. Further, chlorogenic acid may be in the form of a free acid or a physiologically acceptable derivative, in particular a salt or an ester form, and a potassium salt (potassium caffeine etc.) is particularly suitable.

The coffee tree seed extract containing chlorogenic acid of the present invention as an essential component can be used as it is, but when it is blended as a cosmetic, the amount to be prescribed depends on the kind of the cosmetic, the quality of the extract used, and the expectation. Slightly different depending on the degree of action taken. Usually, 0.05 to 30% by weight (hereinafter, represented by% by weight), preferably 3 to 10% is good. If the amount is less than 0.05%, the effect is not sufficient, and even if the amount exceeds 30%, the effect commensurate with the amount cannot be expected.

The cosmetics containing the coffee tree seed extract containing chlorogenic acid as an essential component of the present invention are generally used in cosmetics, pharmaceuticals, quasi drugs, etc. within a range not impairing the effects of the present invention. Various components commonly used, such as oils (animal and vegetable oils, mineral oils, ester oils, wax oils, silicone oils, higher alcohols, phospholipids, fatty acids, etc.), surfactants (anionic, cationic, amphoteric or amphoteric Nonionic surfactant), vitamins (vitamin A)
Group, vitamin B group, folic acid, nicotinic acid, pantothenic acid, biotin, vitamin C group, vitamin D group, vitamin E group, other ferulic acid, γ-oryzanol, etc.),
UV absorbers (p-aminobenzoic acid, anthranil, salicylic acid, coumarin, benzotriazole, tetrazole, imidazoline, pyrimidine, dioxane, furan,
Pyrone, camphor, nucleic acid, allantoin and their derivatives, amino acid compounds, shikonin, baicalin, baicalein, berberine, etc., antioxidants (stearate, nordihydroguaselethenoic acid, dibutylhydroxytoluene, butylhydroxyanisole, para) Hydroxyanisole, propyl gallate, sesamol,
Sesamolin, gossypol, etc.), thickener (hydroxyethyl cellulose, ethyl cellulose, carboxyethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxymethyl cellulose sodium, hydroxypropyl cellulose, nitrocellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, Polyacrylate, carboxyvinyl polymer, gum arabic, gum tragacanth, agar, casein, dextrin, gelatin,
Pectin, starch, alginic acid and its salts), moisturizers (propylene glycol, 1,3-butylene glycol, polyethylene glycol, glycerin, chondroitin sulfate and its salts, hyaluronic acid and its salts, sodium lactate, etc.) Alcohol, polyhydric alcohol, water-soluble polymer, pH adjuster, antiseptic / antifungal agent, colorant, fragrance, cooling agent, stabilizer, animal / plant extract, animal / animal
Vegetable protein and its degradation product, animal / vegetable polysaccharide and its degradation product, animal / vegetable glycoprotein and its degradation product, microbial culture metabolic component, blood flow promoter, anti-inflammatory agent, anti-inflammatory agent, anti-allergic agent , A cell activating agent, an amino acid and its salt, a keratolytic agent, an astringent agent, a wound healing agent, a foaming agent, an oral agent, and a deodorant / deodorant, and they can be used together.

The dosage form of the cosmetic composition containing the coffee tree seed extract containing chlorogenic acid of the present invention as an essential component is arbitrary, and may be prepared by a conventional method, for example, a lotion, cream, ointment, Lotion, emulsion, pack, oil, soap (including medicated soap), face wash, fragrance,
It can be in the form of cologne, bath agent, shampoo, conditioner, hairnic, hair spray and the like.
In addition, various forms of oral drugs, non-woven fabrics such as sanitary cotton and wet tissues, and oral compositions due to stomatitis can be used.

Further, the form of the cosmetic is arbitrary, and it can be used in the form of solution, cream, paste, gel, gel, foam, solid or powder.

The production examples (extraction examples) will be disclosed and described in more detail below, but the method shown by the following is used in the confirmation test of the action and the like described later, and is not limited to this. .

Extraction Example 1: (Extraction with 1,3-butylene glycol) 20 g of coffee tree seeds (green beans) were pulverized to 60%
Add 1,3-butylene glycol aqueous solution (1Kg),
The mixture is stirred and extracted at 5 ° C for 3 hours, cooled, filtered, and then solid-liquid separated.
After passing through a column filled with 1 to adsorb caffeine, the eluate is dried under reduced pressure to obtain about 2.5 g of the coffee tree seed extract of the present invention. Then take 0.5g, 20
% 1,3-butylene glycol aqueous solution was added and dissolved to make 100 ml of the coffee tree seed extract.

Extraction Example 2: (Extraction with ethanol) 20 g of coffee tree seeds (green beans) were crushed to 60%
Aqueous ethanol solution (1Kg) was added, and the mixture was extracted with stirring at 75 ° C for 3 hours, cooled, filtered and subjected to solid / liquid separation, and the separated extract was concentrated under reduced pressure to remove ethanol and then extract the coffee tree seed extract. About 40 ml was obtained.

Extraction Example 3: (Extraction with hot water) 20 g of coffee tree seeds (green beans) were crushed into water (1 K).
g) was added, and the mixture was stirred and extracted at 75 ° C. for 3 hours, cooled, filtered and subjected to solid / liquid separation, and the separated extract was concentrated under reduced pressure to obtain about 40 ml of a coffee tree seed extract.

[0026]

[Examples] Examples will be disclosed and described in more detail below, but the method shown below was used in the confirmation test for the action and the like described later, and is not limited thereto.

Example 1 Determination of Chlorogenic Acid Chlorogenic acid (manufactured by Janssen) was dissolved in an ethanol solution as a standard and the chlorogenic acid contents of Extraction Examples 1 to 3 obtained above were analyzed by high performance liquid chromatography ( It was measured by Shimadzu Corporation. The test results are shown below. Extraction example 1: (Extract with 1,3-butylene glycol) Chlorogenic acid content: 3.8% Extraction example 2: (Extract with ethanol) Chlorogenic acid content: 2.6% Extraction example 3: (Hot water Extraction solution) Chlorogenic acid content: 2.4%

Example 2 Lipid Peroxide Production Inhibitory Action Test TBA method (Analytical, Biochemistry Vol.95, p.351-358, 1)
979), the inhibitory effect of linolenic acid on peroxide generation was examined and measured by the following test method.

[Test Method] 0.1% linolenic acid was added to 0.8% sodium lauryl sulfate aqueous solution and dissolved, and 3.9 ml of this solution was placed in a 10 ml transparent glass screw bottle. 0.1 ml of the sample solution was added to this, and ultraviolet rays (Toshiba: 3 FL-20SE lamps and 3 FL-20SBL lamps were lit in parallel at a distance of 15 cm) were irradiated for 1 hour, and 1 ml of this solution was taken. 1 ml of a mixture of 0.67% thiobarbituric acid aqueous solution and 15% acetic acid aqueous solution (pH 3.5),
Add 20 μl of 4.5% dibutyl hydroxytoluene,
Heat at 95 ° C. for 1 hour. After cooling, add 4 ml of methanol: n-butanol (15:85), shake well, and centrifuge. Next, the absorbance at 534 nm of this n-butanol layer was measured and used as the amount of lipid peroxide. The amount of lipid peroxide when the sample was added and irradiated with ultraviolet light was a, the amount of lipid peroxide when the sample was added and not irradiated with ultraviolet light was b, and the extraction solvent (1,3-butylene) was used instead of the sample. Glycol or ethanol, purified water) is added and the amount of lipid peroxide when irradiated with ultraviolet rays is a ', and the extraction solvent (1,3-butylene glycol or ethanol, purified water) is added instead of the sample and ultraviolet rays are not irradiated. When the lipid peroxide amount in this case was b ′, the lipid peroxide production inhibitory rate was calculated by Equation 1. As the “preparation of sample” sample, the above-mentioned extraction examples 1 to
As Comparative Example 3, 1 g of α-tocopherol (manufactured by Kishida Chemical Co., Ltd.) was dissolved in 100 ml of an ethanol solution and used as a control.

[0030]

[Equation 1]

[0031]

[Table 1]

[Test Results] The results are shown in Table 1. The coffee tree seed extract containing chlorogenic acid of the present invention as an essential component is slightly stronger than the tocopherol solution of Comparative Example 1 or has substantially the same peroxidation. It was confirmed that it exhibited a lipogenesis inhibitory action.

Example 3: Ultraviolet absorption test "Test method" Using a spectrophotometer (1 cm layer length: quartz cell), the absorbance of ultraviolet rays was measured by the absorbance measurement method to determine the ultraviolet absorption effect. The samples used were Extraction Examples 1-2 of Example 2, and the same concentration of para-aminobenzoic acid (PABA) was used as a control.

[0034]

[Figure 1]

[Test Results] As shown in FIG. 1, the coffee tree seed extract containing chlorogenic acid of the present invention as an essential component absorbs ultraviolet light wavelength (280 to 350 nm) predominantly as compared with para-aminobenzoic acid. I was able to confirm that.

Example 4: Tyrosinase activity inhibitory activity test "Test method" tyrosine solution (L-tyrosine 0.3 mg /
1.0 ml, Macklevine buffer (pH 6.
5) Mix 2.0 ml and 0.2 ml of the sample solution,
After leaving for about 10 minutes in a 37 ° C constant temperature bath, add a tyrosinase solution (derived from mushrooms, 2500 unit
/ Ml) 0.1 ml was added, and then the absorbance at 475 nm was measured (A). After that, this reaction solution was allowed to stand for 20 minutes in a 37 ° C. constant temperature bath, and then the absorbance at 475 nm was similarly measured (A). '). At the same time, as a blank, an extraction solvent (1,3-butylene glycol or ethanol, purified water) was added instead of the sample, and the absorbance was measured (a) and (a ') by the same operation to inhibit tyrosinase activity. Rate 2
Sought by. The samples used were Extraction Examples 1 to 3 of Example 1 and Control: Comparative Example 1.

[0037]

[Equation 2]

[0038]

[Table 2]

[Test Results] As shown in Table 2, the coffee tree seed extract containing chlorogenic acid of the present invention as an essential component was compared with the tocopherol solution of Comparative Example 1.
It was confirmed to have a strong tyrosinase activity inhibitory action.

Example 5: Melanin production inhibitory action test Referring to the method described in "Test method" (1) (Cosmetics Technical Journal 26 (1), 8, 1992), C57BL mice (8W♀) were exposed to ultraviolet rays (made by Toshiba). : UV FL20-SE lamp) 15.2 mJ / cm ² , 1 day
Irradiation was repeated once for 3 days. One week after the final irradiation day, the auricle was collected, the cartilage was removed, and the skin of the outer ear was dipped in a 2N-sodium bromide solution. Then, the epidermis was peeled off from the direct skin, and the epidermis was immersed in a 0.1% L-dopa solution (0.1 M phosphate buffer, pH 7.2) for dopa staining. The samples (Extraction Examples 1 to 3) were placed in the dopa solution as shown in Table 3.
20% as a control
A 1,3-butylene glycol aqueous solution was added in the same manner, and the determination was performed by measuring the number of melanocytes in a certain portion (5 mm ² ) with a microscope and evaluating the number of cells per 1 mm ² , and the results are shown in Table 3.

[0041]

[Table 3]

[Test Results] The results are shown in Table 3, and the coffee tree seed extract containing chlorogenic acid of the present invention as an essential component was prepared by treating the melanocytes of the epidermis of colored mouse auricles activated by ultraviolet rays with dopa liquid. When specifically stained with, the decrease in the number of melanocytes (melanocytes) was predominantly observed. Therefore, it was confirmed that it has a melanin production inhibitory action.

Example 6: Tensile Strength Test "Testing Method and Evaluation Method" Fifty untreated human hairs were rinsed with warm water, 50 untreated human hairs were allowed to penetrate into the sample solution for 10 minutes, and then air-dried well. , UV (Toshiba: FL-20SE
Three lamps and three FL-20SBL lamps were arranged in parallel and irradiated at a distance of 15 cm) for 1 hour, finally rinsed with warm water, rinsed off, and dried for 10 minutes to measure tensile strength.
In the measurement, untreated human hair having a uniform length of about 10 cm was pulled one by one at a speed of 2 cm / min, and the load at break was determined. The samples used were Extraction Examples 1 to 3 and Comparative Example 1 of Example 1, and the tensile strength was measured using a tensilon meter (manufactured by Toyo Baldwin) under the conditions of temperature 20 ° C. and humidity 60%. . Numerical values in the table represent average values, and the results are shown in Table 4.

[0044]

[Table 4]

[Test Results] As shown in Table 4, the human hair treated with the coffee tree seed extract containing chlorogenic acid of the present invention as an essential component was treated with Comparative Example 1 (α-tocopherol solution). As compared with human hair, the breaking load showed a higher numerical value, and good results were obtained with a protective effect of suppressing the decrease in the tensile strength of hair due to ultraviolet rays.

Example 7: Persistence of water retention effect "Test method and evaluation method" Human hair (weight: 4 g, length: 10 c)
To m), apply 5 g of the hair rinse shown in Table 5 below, rinse with warm water, rinse with air for 10 minutes, store at constant humidity, and measure the water content immediately after and after 3 hours to obtain the change rate. The sustainability of the effect was evaluated. The evaluation method is
The results were shown in Table 5 by performing a 3-level evaluation of -C. Evaluation A: Change rate of less than 50%. Evaluation B: Change rate of 50% or more and less than 70%. Evaluation C: Change rate of 70% or more.

Example 8 Tactile sensory test (smoothness / moistness / combability) "Testing method and evaluation method" 40 female panelists, 1 in each section
The hair rinses of the present invention product and the comparative product shown in Table 5 below were handed over 5 g each by 0 people, washed with a commercial shampoo, rinsed well with warm water, and then rinsed with hair directly. Was applied and thoroughly permeated, rinsed with warm water, rinsed off, and sensory-evaluated for smoothness after air-drying, moist feeling, and combability. In addition, the evaluation method was performed by the following three-stage evaluation of 1 to 3, and the average value of 10 persons was measured, and the results are shown in Table 5. Evaluation 3: Good. Evaluation 2: Normal. Evaluation 1: Bad.

Example 9: Feeling sensory test (luster of hair) "Test method and evaluation method" 40 female panelists, 1 in each section
The hair rinses of the present invention product and the comparative product shown in Table 5 below were handed over 5 g each by 0 people, washed with a commercial shampoo, rinsed well with warm water, and then rinsed with hair directly. Was applied, thoroughly permeated, rinsed with warm water, rinsed, and air-dried, and a sensory evaluation was made on the gloss of the hair. In addition, the evaluation method was performed by the following three-stage evaluation of 1 to 3, and the average value of 10 persons was measured, and the results are shown in Table 5. Evaluation 3: Shiny. Evaluation 2: There is a gloss. Evaluation 1: Matte.

"Test Results" Hair rinses having the composition shown in Table 5 were prepared, and the durability of the water retention effect of the hair, the smoothness of the hair, the moist feeling, the combability, and the gloss of the hair were evaluated together with the comparative product. The results are shown in Table 5 and shown in Tables 1 to 3 of the present invention.
Is the tensile strength, the retention of moisture on the hair, the smoothness of the hair, the moist feeling, the comb-through, the gloss, both of which are compared to the comparative product.
Overall, good results were obtained.

[0050]

[Table 5]

Example 10: Use effect test "Test method and evaluation method" 40 adult male and female panelists were divided into 4 groups with 10 groups each, and the sunscreen emulsions of the present invention and comparative products shown in Table 6 below were prepared. 30g handed every day,
Before going out, I took an appropriate amount by hand, applied it on my face, and conducted a continuous use effect test for one month.
When the face was washed by sweating, an appropriate amount was taken and applied each time. The evaluation method was based on the following criteria. "Skin aging prevention effect" Effective: The skin's suppleness and gloss were improved. Slightly effective: The skin's elasticity and gloss were slightly improved. Ineffective: No change from before use. "Effect of rough skin improvement" Effective: The roughness and roughness of the skin were improved. Slightly effective: The bulkiness and roughness of the skin are slightly improved. Ineffective: No change from before use. "Skin color improvement effect" Effective: The skin color was improved to white. Slightly effective: The skin color was improved to be slightly white. Ineffective: No change from before use. The numerical values in Table 7 represent the number of people, and the results are shown in Table 7.

[0052]

[Table 6]

"Test Results" As shown in Table 7, the products 1 to 3 of the present invention are improved in prevention of skin aging, rough skin, and complexion.
Compared to the comparative product, overall improvement and good results were obtained, confirming that it was effective.

[0054]

[Table 7]

Example 11: Safety test [Primary skin irritation test] Rabbits with coffee beans seed extracts containing chlorogenic acid as an essential component (Extraction Examples 1 to 3) having their backs dehaired (1 group, 3 animals, body weight) About 3,800 g) was applied to the skin. The evaluation was performed 24, 48, and 72 hours after application by the scoring method for primary irritation using erythema and edema as indexes. As a result, all animals were judged to be negative without any erythema or edema. [Skin cumulative irritation test] Similarly, coffee tree seed extract containing chlorogenic acid as an essential component (Extraction Examples 1 to 1)
3) 1 on the skin of Hartley guinea pigs (female, 5 animals per group, weight 320g) of which the flank was shaved (2 × 4 cm ² ).
0.5 ml / animal was applied once a day, 5 times a week. The application was carried out for 4 weeks, and the hair removal was performed on the last application day of each week.
Judgment was performed on the day after the last day of each week, using erythema and edema as an index by the primary irritation scoring method. As a result, in all animals, erythema and edema were not observed for 1 to 4 weeks after application, and it was determined to be negative. [Acute toxicity test] Similarly, a coffee tree seed extract (extraction examples 1 to 3) containing chlorogenic acid as an essential component, in which the solvent was completely distilled off under reduced pressure, was fasted for 4 hours before the test. 2,000 per strain of mice (5 mice per group, weight 30g)
Oral administration of mg / kg was performed, and the onset and extent of toxic symptoms were observed over time. As a result, no abnormality was observed in all mice for 14 days, and there was no abnormality in the autopsy result. LD
₅₀ was determined to be 2,000 mg / kg or more.

The use of the present invention will be described in more detail with reference to the following examples, but the present invention is not limited to the following examples and is applicable to various cosmetics, pharmaceuticals, quasi-drugs and the like. It can be contained and compounded before use. In addition, each Example may be manufactured by a conventional method for manufacturing each product, and only the compounding amount is shown.

Example 11: Cold Cream Weight% 1. White beeswax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Almond oil 15.0 5. Borax 0.5 6. Coffee tree seed extract (1,3-butylene glycol extract) 3.0 7. Preservative proper amount 8. Perfume proper amount 9. Residue of purified water 100

Example 12: Cream Weight% 1. Squalane 20.0 2. Beeswax 5.0 3. Refined jojoba oil 5.0 4. Glycerin monostearate 2.0 5. Polyoxyethylene (20) sorbitan monostearate 2.0 6. Glycerin 5.0 7. Coffee tree seed extract (30% ethanol extract) 5.0 8. Preservative proper amount 9. Suitable amount of perfume 10. Residue of purified water 100

Example 13: Lotion Weight% 1. Sorbit 2.0 2.1,3-butylene glycol 2.0 3. Polyethylene glycol 1000 1.0 4. Polyoxyethylene oleyl ether (25E.O.) 2.0 5. Ethanol 10.0 6. Coffee tree seed extract (40% propylene glycol extract) 5.0 7. Suitable amount of perfume 8. Residue of purified water 100

Example 14: Cream foundation weight% 1. Stearic acid 4.0 2. Glycerin monostearate 3.0 3. Cetanol 1,5 4. Isopropyl myristate 7.0 5. Liquid paraffin 10.0 6. White beeswax 3.0 8. Kaolin 3.0 9. Talc 1.0 10. Coffee tree seed extract 4.0 (Ethanol: 1,3-Vetylene glycol = 1: 1 extract) 11. Color pigment 1.0 12. Triethanolamine 3.0 13. Glycerin 3.0 14. Bentonite 1.0 15. Preservative proper amount 16. Suitable amount of perfume 17. Residue of purified water 100

Example 15: Shampoo Weight% 1. Lauryl sulfate triethanolamine 5.0% 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3.1,3-butylene glycol 4.0 4. Lauric acid diethanolamide 2.0 5. Disodium edetate 0.1 6. Coffee tree seed extract (water extract) 4.0 7. Preservative proper amount 8. Perfume proper amount 9. Residue of purified water 100

Example 16: Hair Liquid Weight% 1. Ethanol 25.0% 2. Polyoxypropyl ethyl ether phosphate 12.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine ruamide 2.0 5. Disodium edetate 0.1 6. Coffee tree seed extract (30% acetone extract) 4.0 7. Preservative proper amount 8. Perfume proper amount 9. Residue of purified water 100

Example 17: Heartonic Weight% 1. Ethanol 35.0% 2. Ethyl oleate 2.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. Coffee tree seed extract (ethanol extract) 5.0 5. Perfume proper amount 6. Residue of purified water 100

Example 18: Bath agent Weight% 1. Sodium hydrogen carbonate 60.0 2. Anhydrous sodium sulfate 32.0 3. Borax 3.0 4. Dry powder of coffee tree seed extract 6.0 (Ethanol: 1,3-butylene glycol: water = 1: 1: 1 extract)

[0065]

The coffee tree seed extract containing chlorogenic acid as an essential component of the present invention has a lipid peroxide production inhibitory action, an ultraviolet absorbing action, and a melanin production inhibitory action, and therefore is contained in cosmetics. If used, it can be expected to have a whitening effect, prevent skin aging, and protect hair. The fields of use thereof include various cosmetic compositions in the fields of pharmaceuticals / quasi-drugs or cosmetics (various preparations used for humans and other animals), specifically, lotions, emulsions, It is applied to creams (including ointments), oils, packs, soaps (including medicated soaps), body soaps, bath agents, shampoos, rinses, hairnics, hair sprays, etc.

[Brief description of drawings]

1 is a spectrophotometer (1 cm layer length: quartz cell),
It is a graph which measured the light absorbency of ultraviolet rays by the light absorbency measuring method. In addition, as the sample, the extraction examples 1-2 of Example 2 were used, and as a control, a para-aminobenzoic acid (PABA) solution having the same concentration was used.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical indication location A61K 7/42 7/50 35/78 ADA 8217-4C ADD 8217-4C ADS C 8217-4C (72 ) Inventor Kuki Hirasawa 5-1-6 Tamon-dori, Chuo-ku, Kobe-shi, Hyogo UCC Techno Development Co., Ltd. In the company

Claims (3)

[Claims] 1. Containing chlorogenic acid as an essential component,
A whitening cosmetic, comprising an extract obtained by using one or more solvents selected from water, lower alcohols and polyol organic solvents of coffee tree seeds. 2. Containing chlorogenic acid as an essential component,
A skin anti-aging cosmetic composition comprising an extract obtained by using one or more solvents selected from water of coffee tree seeds, lower alcohols and polyol organic solvents. 3. Containing chlorogenic acid as an essential component,
A cosmetic for protecting hair, comprising an extract obtained by using one or more solvents selected from water, lower alcohols and polyol organic solvents of coffee tree seeds.