JPH03134000A - Method for extracting polysaccharide from fruit body of polyporus confluens and antitumor agent containing same polysaccharidea as active ingredient - Google Patents
Method for extracting polysaccharide from fruit body of polyporus confluens and antitumor agent containing same polysaccharidea as active ingredientInfo
- Publication number
- JPH03134000A JPH03134000A JP1270483A JP27048389A JPH03134000A JP H03134000 A JPH03134000 A JP H03134000A JP 1270483 A JP1270483 A JP 1270483A JP 27048389 A JP27048389 A JP 27048389A JP H03134000 A JPH03134000 A JP H03134000A
- Authority
- JP
- Japan
- Prior art keywords
- polysaccharide
- xyloglucan
- solvent
- active ingredient
- antitumor agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000004676 glycans Chemical class 0.000 title claims abstract description 24
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 24
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 5
- 239000002246 antineoplastic agent Substances 0.000 title claims description 7
- 239000004480 active ingredient Substances 0.000 title claims description 6
- 241001199091 Albatrellus confluens Species 0.000 title abstract 3
- 235000013399 edible fruits Nutrition 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 10
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 10
- 229920002000 Xyloglucan Polymers 0.000 claims abstract description 8
- 239000006228 supernatant Substances 0.000 claims abstract 6
- 239000002904 solvent Substances 0.000 claims abstract 5
- 238000002523 gelfiltration Methods 0.000 claims abstract 3
- 238000010828 elution Methods 0.000 claims abstract 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 3
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims 3
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims 3
- 239000008103 glucose Substances 0.000 claims 3
- 239000000203 mixture Substances 0.000 claims 3
- 244000046146 Pueraria lobata Species 0.000 claims 2
- 238000001914 filtration Methods 0.000 claims 2
- 102000007474 Multiprotein Complexes Human genes 0.000 claims 1
- 108010085220 Multiprotein Complexes Proteins 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000003809 water extraction Methods 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 239000003699 antiulcer agent Substances 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
- 230000000259 anti-tumor effect Effects 0.000 description 5
- 241000221198 Basidiomycota Species 0.000 description 2
- 241000019462 Colpodium versicolor Species 0.000 description 2
- 240000000599 Lentinula edodes Species 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 235000001715 Lentinula edodes Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000006268 Sarcoma 180 Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE:To obtain a polysaccharide as xyloglucan conjugated protein useful as an antiulcer agent by extracting a fruit body of Polyporus Confluens with a solvent, extracting the residue with hot water, separating the supernatant, adding a solvent to the supernatant to precipitate the polysaccharide and subjecting the polysaccharide to gel filtration to fractionate the polysaccharide. CONSTITUTION:A fruit body of Polyporus Confluens is extracted with an organic solvent and solvent-soluble fraction is filtered and removed and the extracted residue is further extracted with hot water. The extract solution is cooled and then supernatant thereof is separated and the solution is concentrated to high concentration and then equivalent amount of solvent is added thereto to precipitate polysaccharide and the supernatant is separated to afford polysaccharide. The polysaccharide is further fractionated by elution treatment by gel filtration method to provide the polysaccharide as xyloglucan conjugated protein having different ingredient content.
Description
【発明の詳細な説明】
（イ）本発明の目的について
〈産業上の利用分野〉
本発明はニンギョウタケ（Ｐｏｌｙｐｏｒｕｓ Ｃｏｎ
ｆｌ−ｕｅｎｓ）の子実体に存在し抗腫瘍性を有する多
糖体（キシログルカン蛋白複合体）を有効成分とする抗
腫瘍剤とその抽出方法に関するものである。DETAILED DESCRIPTION OF THE INVENTION (a) Object of the present invention <Industrial application field>
The present invention relates to an antitumor agent whose active ingredient is a polysaccharide (xyloglucan protein complex) that exists in the fruiting body of S. fl-uens and has antitumor properties, and a method for extracting the same.
〈従来の技術について〉 担子菌由来のカワラタケ、スエヒロタケ。<About conventional technology> C. versicolor, Suehirotake, derived from basidiomycetes.
シイタケ、マンネンタケなどの抗腫瘍性多糖体はマウス
皮下に移植されたＳ ａｒｃｏｍａ−１８０固型癌の成
長を強く抑制することで知られている。しかし、Ｃ３Ｈ
／ｆ、Ｃ３Ｈ／Ｈａ系などの組織適合抗原Ｈ−２′Ｉを
もつ動物では抗腫瘍活性は全く無効であるか極めて弱い
とされている。Antitumor polysaccharides such as shiitake mushrooms and stone mushrooms are known to strongly suppress the growth of Sarcoma-180 solid tumors subcutaneously transplanted into mice. However, C3H
Antitumor activity is said to be completely ineffective or extremely weak in animals with histocompatibility antigen H-2'I, such as C3H/f and C3H/Ha systems.
又抗腫瘍剤としては多くの化学合成薬剤が使用されてい
る。Furthermore, many chemically synthesized drugs are used as antitumor agents.
〈発明が解決しようとする問題点について〉上記のよう
に担子菌由来のカワラタケ、スエヒロタケ、シイタケ、
マンネンタケなどがら得られる抗腫瘍性多糖体はＣ３Ｈ
／ｆ、Ｃ３Ｈ／Ｈｅ系などの組織適合抗原Ｈ−２１をも
つものには抗腫瘍活性は全く無効か極めて弱く、又化学
合成薬剤においては副作用が多く且つ大であるという問
題点が存在する。<Problems to be solved by the invention> As mentioned above, C. versicolor, Suehirotake, and Shiitake, which are derived from basidiomycetes,
Anti-tumor polysaccharide obtained from C3H, etc.
/f, C3H/He series and other histocompatibility antigen H-21 have no or very weak antitumor activity, and chemically synthesized drugs have many and serious side effects.
Claims (5)
溶画分を濾過除去してその抽出残渣を更に熱水抽出を行
い、これを冷却して後上澄液を分離し、次いで当該液に
高濃度にして等量の溶媒液を加えて多糖体を沈殿させそ
の上澄を分離して多糖体を得、更に当該多糖体をゲル濾
過法による溶出操作によって分別し、含有組成分量の異
るキシログルカン蛋白複合体としての多糖体を得ること
を特徴とするニンギョウタケ子実体からの多糖体抽出方
法。(1) Extract the fruiting body of Ningyotake with a solvent solution, remove the solvent-soluble fraction by filtration, and then perform hot water extraction on the extraction residue, cool it, separate the supernatant liquid, and then remove the solvent-soluble fraction by filtration. The polysaccharide is precipitated by adding an equal amount of highly concentrated solvent solution to the solution, and the supernatant is separated to obtain the polysaccharide.The polysaccharide is further fractionated by an elution operation using gel filtration method to determine the difference in the amount of the contained components. 1. A method for extracting polysaccharides from the fruiting body of N. chinensis, which is characterized in that the polysaccharides are obtained as xyloglucan protein complexes.
ログルカン蛋白複合体である多糖体を有効成分とする抗
腫瘍剤。(2) An antitumor agent containing as an active ingredient a polysaccharide, which is a xyloglucan protein complex obtained from an extract of the fruiting body of N. chinensis.
質２．４％、糖組成中グルコースとキシロースとの比が
１００：３．１からなる糖９６％を含有するキシログル
カン蛋白複合体としての多糖体を有効成分とする抗腫瘍
剤。(3) A xyloglucan protein complex containing 2.4% protein obtained from the polysaccharide according to claim (1) and 96% sugar in which the ratio of glucose to xylose in the sugar composition is 100:3.1. An antitumor agent whose active ingredient is a polysaccharide.
白質２．４％、糖組成中グルコースとキシロースとの比
が１００：０．９からなる糖７３％を含有するキシログ
ルカン蛋白複合体としての多糖体を有効成分とする抗腫
瘍剤。(4) A xyloglucan protein complex obtained from the polysaccharide according to claim (1), containing 2.4% protein and 73% sugar in which the ratio of glucose to xylose in the sugar composition is 100:0.9. An antitumor agent whose active ingredient is polysaccharide.
質３．５％、糖組成中グルコースとキシロースとの比が
１００：０からなる糖７９％を含有するキシログルカン
蛋白複合体としての多糖体を有効成分とする抗腫瘍剤。(5) As a xyloglucan protein complex containing 3.5% protein obtained from the polysaccharide according to claim (1) and 79% sugar in which the ratio of glucose to xylose in the sugar composition is 100:0. An antitumor agent containing polysaccharide as an active ingredient.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1270483A JPH03134000A (en) | 1989-10-19 | 1989-10-19 | Method for extracting polysaccharide from fruit body of polyporus confluens and antitumor agent containing same polysaccharidea as active ingredient |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1270483A JPH03134000A (en) | 1989-10-19 | 1989-10-19 | Method for extracting polysaccharide from fruit body of polyporus confluens and antitumor agent containing same polysaccharidea as active ingredient |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03134000A true JPH03134000A (en) | 1991-06-07 |
Family
ID=17486924
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1270483A Pending JPH03134000A (en) | 1989-10-19 | 1989-10-19 | Method for extracting polysaccharide from fruit body of polyporus confluens and antitumor agent containing same polysaccharidea as active ingredient |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03134000A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5261214A (en) * | 1975-11-14 | 1977-05-20 | Kureha Chem Ind Co Ltd | Preparation of anti-tumor substance |
-
1989
- 1989-10-19 JP JP1270483A patent/JPH03134000A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5261214A (en) * | 1975-11-14 | 1977-05-20 | Kureha Chem Ind Co Ltd | Preparation of anti-tumor substance |
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