JP7177697B2 - Non-toxic botanical pharmaceutical compositions and methods and uses thereof - Google Patents

Description

This application claims the priority benefit and filing date of U.S. Provisional Patent Application No. 62/208,662, filed Aug. 22, 2015, the contents of which are hereby incorporated by reference in their entirety. incorporated into the specification.

Agriculture is the most important thing for the world. Agriculture is not only essential to feeding the world, it is also of vital economic importance to the economies of most, if not all, countries. Three factors that can affect crop yields are plant diseases, unfavorable growing conditions and inefficient cultivation.

Losses due to infectious plant diseases can have a devastating humanitarian impact, where crop losses lead to starvation, famine and starvation. In addition, plant disease losses can have significant economic impact, reducing incomes for crop growers and sellers and increasing prices to consumers. Annual loss rates of 30% to 50% of staple crops are common in situations where there are no or few methods of controlling infectious plant diseases. Agricultural productivity has been improved by conventional botanical drug technology based on pesticides. However, the use of pesticides is plagued by negative effects, such as an increased burden on consumers and reduced income for growers and marketers of crops. Furthermore, there is growing public concern about the adverse effects of pesticides on the environment. Therefore, prevention of plant diseases in agriculturally important crops is extremely important for improving crop yields.

In addition, crop plants that are integrated into various ecosystems around the world are exposed to unfavorable growing conditions that adversely affect plant health and vitality. Such less than ideal conditions usually include soil or climatic conditions or extreme temperatures, adverse relationships between moisture and oxygen, toxic substances in the soil or air and excesses or deficiencies of essential minerals. caused by various stresses. Even under favorable growing conditions, such factors can reduce crop productivity to varying degrees. Therefore, improving the growing conditions of agriculturally important crops is extremely important for improving crop yields.

Finally, the depletion of agricultural land as the world's population grows increases pressure to not only optimize crop productivity, but also increase cultivation efficiency. Moreover, as global population growth continues to accelerate and agricultural land continues to shrink, the need to increase crop yields will only increase. Therefore, increasing the productivity of agriculturally important crops is essential for improving crop yields.

Therefore, whether the plants are stressed by plant diseases, poor growing conditions, or even if the plants are healthy and/or growing under favorable conditions, there is still a need to improve cultivation efficiency and productivity. There is a great need for environmentally benign treatment methods that enhance plant health and vitality, regardless of whether they are used. Such treatment methods should also reduce, if not eliminate, pesticides in order to protect human welfare and the environment.

Aspects herein disclose botanical pharmaceutical compositions. The botanical pharmaceutical compositions of the present disclosure comprise treated fermented microbial supernatants and one or more nonionic surfactants. The botanical pharmaceutical compositions of this disclosure may further comprise one or more anionic surfactants. The botanical pharmaceutical compositions of the present disclosure are biodegradable and nontoxic to humans, mammals, plants and the environment.

Aspects herein disclose a botanical drug kit. A botanical drug kit of the present disclosure includes a botanical drug composition disclosed herein and instructions on how to use the same to improve or optimize plant health and/or vigor.

Aspects herein disclose methods of controlling plant diseases. The methods of the present disclosure comprise applying an effective amount of a botanical composition disclosed herein to one or more plants and/or to one or more loci where control of plant diseases is desired. .

Aspects herein disclose methods of increasing plant growth and/or fruit production. The methods of the present disclosure include applying an effective amount of a botanical composition disclosed herein to one or more plants and/or one or more loci where increased plant growth and/or fruit production is desired. Including applying things.

Aspects herein disclose the use of botanical compositions for controlling plant diseases. Uses of the present disclosure include applying the botanical compositions disclosed herein to one or more plants and/or to one or more loci where control of plant diseases is desired.

Aspects herein disclose the use of botanical compositions to increase plant growth and/or fruit production. The use of the present disclosure involves applying an effective amount of a botanical pharmaceutical composition disclosed herein to one or more plants and/or one or more loci where increased plant growth and/or fruit production is desired. Including applying things.

(detailed explanation)
A plant becomes sick when it is constantly disturbed by some pathogen that causes abnormal physiological processes that interfere with its normal structure, growth, function or other activity. Such interference with one or more of the plant's essential physiological or biochemical systems induces characteristic pathologies or symptoms. Plant diseases are caused by pathogenic organisms such as fungi, bacteria, mycoplasma, viruses, viroids, nematodes and the like. An infectious agent is contagious, reproduces within its host, and is capable of spreading from one susceptible host to another. Plant diseases can be broadly classified according to the nature of their primary pathogens. Such primary pathogens include viruses as well as microorganisms such as fungi and bacteria and animals such as nematodes. However, one of the complications in treating plant diseases caused by such primary pathogens is that they are usually protected from the environment by some structure. Such defense structures are not only essential to maintaining the health of the pathogen, but also serve to shield it from compounds designed to destroy it.

Complete virus particles, known as virions, consist of nucleic acid surrounded by a protective protein coat called a capsid. The capsid surrounds the viral genetic material and is composed of proteins and multiple oligomeric structural subunits called protomers. Some viruses are enveloped, ie, the capsid is covered with a lipid membrane known as the viral envelope. This coat is what the capsid secures from the intracellular membrane of the viral host, examples of such intracellular membranes being the inner nuclear membrane, the Golgi membrane and the outer membrane of the cell.

Microorganisms such as bacteria, mycoplasma (bacteria without a bacterial wall) and certain fungi generally secrete polymeric masses of biomacromolecules consisting of extracellular nucleic acids, proteins and polysaccharides, which are called extracellular polymeric substances (EPS). form a matrix. Cells are embedded in this EPS matrix and adhere to each other, as well as to any viable (biological) or non-viable (non-living) surface to form a sessile microbial population called a biofilm or mucus layer. to form Biofilm colonies may form on solid substrates that are immersed or exposed to aqueous solutions, or may be mats that float on the liquid surface.

There are five stages of biofilm development: initial attachment, irreversible attachment, maturation I, maturation II and dispersal. Biofilm formation initially begins with the adherence of planktonic planktonic microorganisms to surfaces. This initial fixation initially adheres to the surface via weak reversible adhesion due to van der Waals forces. If the colonies do not detach from the surface within a short period of time, they are permanently anchored (irreversible attachment) by the secretion of the EPS matrix and the formation of cell adhesion structures such as pili. Once colonization begins, biofilms grow (maturation I and II) through a combination of cell division and new recruitment of the embedded microbes. In addition to extracellular biopolymers secreted by microorganisms, biofilms can also uptake materials from the surrounding environment, including, but not limited to, minerals, soil particles, and biological constituents. Maturation I and II are stages in which the biofilm is established and can change only in shape and size. The final stage of biofilm formation is known as dispersal, during which microorganisms are released from the biofilm and enter a planktonic growth phase where they disperse and settle on new surfaces.

Microorganisms living in biofilms are physiologically different from planktonic planktonic microorganisms of the same species, and have very different characteristics. One reason for such differences is that biofilms protect microorganisms from the environment and allow them to cooperate and interact in various ways. For example, biofilms have been shown to increase the resistance of microorganisms to detergents and antibiotics. Furthermore, horizontal gene transfer is greatly facilitated within the biofilm, increasing the stability of the biofilm structure. Additionally, microorganisms within biofilms can communicate with each other via quorum sensing (QS) using products such as N-acyl homoserine lactones (AHL). Biofilms therefore play an essential and vital role in protecting microorganisms by shielding them from potentially dangerous interactions with the environment.

Organisms larger than microorganisms are also protected from the environment by some structure. Nematodes have a cuticle, which is a polymeric, proteinaceous extracellular matrix. The cuticle of the nematode consists of a mostly multinucleate epithelial cell layer called the hypodermis that secretes various proteins from its apical membrane, which are then extensively cross-linked by peroxidases on the outer surface of the hypodermis to form the cuticle. formed when The main components of this flexible cuticle are members of the collagen superfamily and cuticulin, a highly cross-linked, insoluble protein. Enveloping the cuticle is a three-layered epicuticle rich in lipids, which itself is covered by a negatively charged coat (or glycocalyx) rich in loosely bound glycoproteins. This multifunctional extracellular structure serves as a highly impermeable barrier that protects the nematode from desiccation and pathogenic infection, as well as a structural framework that maintains the nematode's body morphology and integrity and protects the environment. Prevents mechanical damage from invasion from the body and allows locomotion through attachment to body wall muscles. Thus, the nematode cuticle plays an essential and vital role in maintaining the nematode's integrity and interaction with the environment.

Thus, defense structures found in primary pathogens of plant diseases, such as viruses, bacteria, fungi and nematodes, are not only essential for the survival of these pathogens, but also protect them from the environment. . Therefore, a treatment method that interferes with or destroys the defense structures of primary pathogens of plant diseases would be highly beneficial.

Plants, or chlorophytes, are multicellular eukaryotic organisms belonging to the kingdom Plantae and forming the Phylum Chlorophyta. Green plants include flowering plants, conifers and other gymnosperms, pteridophytes, lycopods, hornworts, bryophytes, mosses and green algae, but also red algae, brown algae, fungi, archaea, bacteria and Animals are not included. Plants are characterized by obtaining most of their energy from sunlight through photosynthesis using chloroplasts. Chloroplasts contain chlorophyll a and chlorophyll b, giving them a green color. Plants are also characterized by thick cell walls of cellulose, central vacuoles for storage, plastids for storing pigments, sexual reproduction, modular and infinite growth and alternation of generations, although asexual reproduction is also common.

A typical plant is structurally composed of two major compartments, the root system and the shoot system. The root system is usually underground and includes the taproot and lateral roots, as well as tubers and rhizomes formed by structural deformation of the stem. This system functions to anchor plants to the soil, absorb water and nutrients from the soil, transport water and nutrients throughout the plant, store food, and secrete certain hormones. The shoot system is usually above ground and includes stems, leaves and reproductive organs. This system functions to lift plants above the soil, carry out photosynthesis, reproduce, transport water and nutrients throughout the plant, store food, and secrete certain hormones.

Plants with vascular tissue that distributes resources throughout the plant are called vascular plants. Vascular plants, also known as vascular plants, consist of lignified vascular tissue (xylem) that conducts water and minerals throughout the plant and specialized non-lignified vascular tissue (sytem) that conducts photosynthetic products. are defined as terrestrial plants with Vascular plants include lycophytes, horsetails, ferns, gymnosperms (including conifers) and angiosperms (flowering plants). Scientific names for this group include Tracheophyta and Tracheobionta.

Xylem is a vascular tissue composed of dead cells at maturity. Xylem unidirectionally transports xylem sap from the roots throughout the plant. Xylem sap contains water, soluble mineral nutrients and inorganic ions, and may also contain several other organic chemicals. Movement of xylem sap within the xylem is passive and relies on capillary action to create forces that establish an equilibrium configuration that opposes gravity. This capillary action occurs primarily through two mechanisms: transpirational suction and root pressure. Transpirational suction is the force generated by the evaporation of water from the cell surfaces of the leaves, which creates surface tension and negative pressure within the xylem, sufficient to draw up xylem sap from the roots and soil. is due to the generation of Root pressure is due to positive pressure generated by osmotic action caused by higher solute concentration and negative water potential in root cells than in soil. Climb the wood toward

The phloem contains 1) conducting cells that form tubes and are called phloem elements; 2) parenchymal cells, including both specialized companion cells or protein cells and non-specialized cells; and 3) responsible for mechanical support. It contains living vascular tissue, which consists of supporting cells such as fibers and pallidum cells. The phloem element has no nucleus and few organelles, so it relies on companion or protein cells for most of its metabolic needs. The phloem stores the photosynthetic products (or sap) made in the photosynthetic area of the plant (primarily the leaves) and any other plant parts that require it, especially the non-photosynthetic parts of the plant such as roots or tubers or bulbs. multi-directional transport to storage structures such as Photosynthetic products are aqueous solutions rich in sugars and other soluble organic nutrients produced during photosynthesis. Movement of photosynthates within the phloem is driven by positive hydrostatic pressure. This process is called translocation and is carried out by a process called phloem unloading. Cells in the sugar source "load" the sieve elements by actively transporting solute molecules into the sieve elements. This causes water to move into the sieve element by osmotic action, creating pressure that pushes down the sap within the tube. At the glucose acceptor, the cell actively transports solutes from the sieve element to produce the opposite effect.

The root system is the plant organ, usually below the surface of the soil. Structurally, the root consists of epidermis, cortex, endothelium, inner sheath and vascular system. The epidermis is the outer cell layer. The cortex is the major structural tissue of the root, bordered on the outside by the epidermis and on the inside by the endothelium. The endothelium is the tissue that separates the cortex and the inner sheath, from which the lateral roots (or forks) arise. The core of the root is a vascular tissue consisting of xylem and phloem. The root system includes the taproot, lateral roots and root hairs and is divided into three growing regions. The maturation zone is the portion of the root system and includes the mature portion of the taproot, lateral roots and root hairs that absorb water and nutrients from the soil and transport them through the xylem into the shoot system. Elongation zones are where newly dividing cells grow. The meristematic zone, consisting of the root meristem and root cap, is the area where cell division and new cell proliferation occur.

Root hairs are single absorptive cells that are elongated root epidermal cells. This tiny ciliary structure serves as the primary site for water and mineral uptake. There are beneficial microorganisms that associate with root hairs and form beneficial symbiotic relationships with plants. Mycorrhizae are soil fungi and are thought to increase the contact area between the roots and the soil surface to increase water and nutrient uptake. Rhizobium is a type of soil bacterium that normally forms nodules in plant roots, thereby making atmospheric nitrogen available to plants.

Proper transport of both xylem sap and photosynthetic products is essential for plant survival. Acceleration of this transport process would therefore benefit plant health. For example, improved uptake by root hairs increases the amount of water, minerals and other nutrients required for plant growth. In a similar vein, improved xylem sap and photosynthate flow within the vascular tissue results in effective and efficient synthesis of the compounds and energy required to maintain and continue plant growth.

In contrast, obstacles that impede or disrupt the movement of xylem sap and photosynthates affect plant health. For example, when transpirational suction is impeded by high temperature, high humidity, darkness or drought, the negative water pressure in the xylem is greatly reduced, impeding the flow of xylem sap. Similarly, when unfavorable environmental conditions impede the uptake of water and nutrients by the root hairs and impede root pressure, the positive water pressure in the xylem is greatly reduced, impeding the flow of xylem sap. can come As another example, disruption of photosynthetic product flow in the phloem impairs nutrient distribution. In any of the above cases, such flow disruption can lead to wilting, wilting, stunted growth and reduced fecundity, as well as increased susceptibility to plant disease and adverse environmental conditions. When it comes to agriculture, such disruption of flow ultimately leads to reduced crop yields. Therefore, a treatment method that promotes, maintains or enhances xylem sap and photosynthetic product flow in the xylem and phloem, respectively, would be highly beneficial.

Irrigation is the artificial application of water to land or soil. Irrigation is used in arid regions during periods of inadequate rainfall to promote crop cultivation, landscape maintenance and revegetation of degraded soils. There are also several other uses in crop production, such as protecting plants from frost, controlling weed growth and preventing soil compaction. In contrast, agriculture that relies only on direct rainfall is called rain-fed or dry land agriculture.

The purpose of irrigation is to distribute water evenly throughout a field so that each plant receives just the amount of water it needs, neither too much nor too little. Overhead irrigation, or sprinkler irrigation, is the practice of distributing water under high pressure through a network of pipes to one or more centers within a field and distributing it by means of overhead sprinklers or spray guns. In addition, sprinklers can be mounted on platforms that can be moved manually or automatically to different areas of the field. Types of overhead irrigation include center pivot irrigation, mobile sprinkler irrigation, lateral displacement irrigation and wheelline irrigation. Localized irrigation is the practice of distributing water under low pressure through a network of pipes in a predetermined pattern and applying it to individual plants or adjacent plants in small releases. Types of local irrigation methods include drip irrigation, spray or micro-sprinkler irrigation and bubbler irrigation. Local irrigation is the most efficient use of water, as it supplies only the required amount of water and minimizes evaporation and runoff.

Most commercial residential irrigation systems are "underground" systems, meaning that everything is buried in the ground. Concealed pipes, sprinklers, emitters (drippers) and irrigation valves eliminate watering hoses or other implements that must be moved by hand, making the landscape cleaner and more presentable. However, this creates some disadvantages in managing fully populated systems.

Some problems can arise in irrigation. For example, the plumbing networks of overhead and local irrigation systems can become clogged by the growth of algae and other microorganisms forming biofilms, causing water distribution malfunctions. Such poor water distribution can create unfavorable growing conditions and adversely affect plant health and vigor. For example, inconsistent water distribution can lead to under- or over-irrigation of some fields due to imbalances in the uniform distribution, and under-irrigation increases soil salinity and is detrimental to the soil surface. Salt deposits lead to crop failures due to under- or over-irrigation, and plant diseases are prevalent. Accordingly, a treatment method to promote, maintain or enhance water flow in local and overhead irrigation systems would be highly beneficial.

Without wishing to be bound by theory, the botanical pharmaceutical compositions of the present disclosure dissolve one or more components of defense structures present in pathogens of plant diseases such as viruses, bacteria, fungi and nematodes. Killing of the pathogen is effected by dispersing, dispersing, or otherwise destroying, thereby disrupting one or more essential physiological processes. This mechanism of action is that the botanical pharmaceutical compositions disclosed herein are capable of rupturing, such as breaking, viral capsids, microbial biofilms and nematode cuticle lipid-based epicuticular layers. has something to do with The method of applying the disclosed botanical pharmaceutical compositions is such that one or more components of the defense structures present in the pathogen are sufficiently disrupted that the pathogen is then killed by disruption of essential physiological processes. By direct application to the pathogen, indirectly by treating the locus where the pathogen is exposed to the disclosed botanical composition, or any other method of exposing the pathogen to the disclosed botanical composition. by external exposure.

Additionally, without wishing to be bound by theory, the botanical pharmaceutical compositions of the present disclosure improve absorption by root hairs, improve xylem sap flow through the xylem, and improve photosynthetic production in the phloem. There is improved transport of water and nutrients that improves logistics and thereby maintains and/or enhances plant health and vitality. This mechanism of action is that the botanical pharmaceutical compositions disclosed herein increase the absorption of water, minerals and other nutrients from the soil, increase capillary action and/or hydrostatic pressure in the xylem, and/or being able to increase synthesis of compounds and energy, thereby maintaining and continuing plant growth, and/or enhancing plant health and vitality. The methods of applying the disclosed botanical pharmaceutical compositions provide improved absorption of water, minerals and other nutrients, improved transport of these materials throughout the plant, and/or improved maintenance and continuation of plant growth. By direct application to the plant, indirectly by treating the locus where the plant is exposed to the disclosed botanical pharmaceutical compositions, or by treating the plant with the disclosed botanical pharmaceutical compositions so that synthesis of the required compounds and energy is improved. External exposure is carried out by any other method of exposing to the botanical drug composition of .

Similarly, without wishing to be bound by theory, the botanical pharmaceutical compositions of the present disclosure inhibit xylem sap flow at the xylem and/or photosynthetic product flow at the phloem. Multiple components are dissolved, dispersed, or otherwise removed, resulting in improved water and nutrient transport that maintains and/or enhances plant health and vitality. This mechanism of action is due to the fact that the botanical pharmaceutical compositions disclosed herein dissolve or otherwise remove one or more components that block the passage of the xylem and phloem. is related to the possibility of The method of applying the disclosed botanical pharmaceutical compositions is such that the one or more components that inhibit xylem sap flow and/or photosynthetic product flow are sufficiently destroyed and then water and nutrient transport throughout the plant is permitted. ameliorated by direct application to the plant, indirectly by treating the locus where the plant is exposed to the disclosed botanical composition, or by exposing the plant to the disclosed botanical composition. external exposure by any other method. In one embodiment, the one or more components that inhibit xylem sap flow and/or photosynthetic product flow include biofilms.

Additionally, without wishing to be bound by theory, the botanical compositions of the present disclosure dissolve or disperse one or more components that inhibit water flow within the pipeline network of the perfusion system. or otherwise removed, resulting in improved water distribution that maintains and/or enhances plant health and vitality. This mechanism of action is that the botanical drug compositions disclosed herein are capable of dissolving or otherwise removing one or more components that inhibit the pipeline network. has something to do with A method of applying the disclosed botanical drug compositions is to improve the irrigation system so that one or more components that inhibit the pipeline network are sufficiently removed and then water transport throughout the pipeline network is improved. External exposure is carried out by direct application to the pipeline network or by any other method of exposing the pipeline network to the botanical drug composition of this disclosure. In one embodiment, the one or more components that impede the pipeline network include biofilms.

Regardless of theory of operation, the botanical pharmaceutical compositions and methods and uses of the present disclosure provide an alternative that does not rely on chemicals that are toxic to humans or the environment. Rather, the botanical pharmaceutical compositions and methods and uses disclosed herein utilize innate processes to improve absorption and transport of raw materials as well as release compounds and energy to sustain growth. Acts to improve synthesis. Similarly, the botanical pharmaceutical compositions and methods and uses disclosed herein exploit the vulnerability of the pathogen itself to reduce its environment, the plant's xylem sap flow and/or photosynthetic product flow. It acts on one or more components that impede or impede the water flow of an irrigation system. Additionally, the components of the botanical compositions of the present disclosure have been found to be substantially non-toxic to humans and domestic animals and to have minimal adverse effects on wildlife and the environment.

Aspects of the present specification disclose, in part, botanical pharmaceutical compositions. The botanical pharmaceutical compositions disclosed herein comprise treated fermented microbial supernatants and one or more nonionic surfactants. No live microorganisms such as yeast or bacteria are present in the treated fermented microbial supernatant, nor are there any active enzymes or activatable proenzymes, and no enzymatic activity. . Furthermore, the botanical composition itself does not contain any live microorganisms such as yeast or bacteria, nor does it contain any active enzymes or activatable proenzymes, nor any enzymatic activity. do not have.

In one aspect of this embodiment, the botanical composition disclosed herein comprises, for example, from about 75% to about 99% of the treated fermented microbial supernatant and from about 1% to 25% of one or and a plurality of nonionic surfactants. In another aspect of this embodiment, the botanical pharmaceutical composition disclosed herein comprises, for example, from about 80% to about 97% of the treated fermented microbial supernatant and from about 3% to 20% of one or multiple nonionic surfactants. In yet another aspect of this embodiment, the botanical compositions disclosed herein comprise, for example, from about 85% to about 95% treated fermented microbial supernatant and from about 5% to 15% 1 and one or more nonionic surfactants. In yet another aspect of this embodiment, the botanical pharmaceutical composition disclosed herein comprises, for example, from about 87% to about 93% of the treated fermented microbial supernatant and from about 7% to 13% of 1 and one or more nonionic surfactants. In another aspect of this embodiment, the botanical composition disclosed herein comprises, for example, from about 88% to about 92% of the treated fermented microbial supernatant and from about 8% to 12% of one or multiple nonionic surfactants. In another aspect of this embodiment, the botanical pharmaceutical composition disclosed herein comprises, for example, from about 89% to about 91% of the treated fermented microbial supernatant and from about 9% to 11% of one or multiple nonionic surfactants.

Aspects herein disclose, in part, a fermented microbial supernatant. The fermented microbial supernatant disclosed herein is prepared by culturing a yeast strain, a bacterial strain or a combination of both yeast and bacterial strains in a fermentation medium comprising a sugar source, malt and magnesium salts. can be done. In one aspect of this embodiment, only a single yeast strain is used in the fermentation medium. In another aspect of this embodiment, two or more different yeast strains are used in the fermentation medium. In yet another aspect of this embodiment, only a single bacterial strain is used in the fermentation medium. In yet another aspect of this embodiment, two or more different bacterial strains are used in the fermentation medium. In another aspect of this embodiment, one or more different yeast strains are used with one or more different bacteria in the fermentation medium. In yet another aspect of this embodiment, 2, 3, 4, 5 or more different yeast strains are combined with 2, 3, 4, 5 or more different bacteria in the fermentation medium. use.

Sugar sources include, but are not limited to, sucrose from molasses, cane raw sugar, soybeans or mixtures thereof. Molasses generally contains, in addition to reducing sugars such as glucose, up to about 50% sucrose, as well as maltase and ash, organic non-sugars and small amounts of water. The presence of sugars of the type present in molasses is important in promoting the activity of the enzyme and the yeast that produces it. Untreated cane molasses is preferred as the natural source of raw materials required for enzymatic fermentation, but other molasses such as sugar beet molasses and barrel molasses may also be used. The amount of molasses useful in preparing the fermentation media disclosed herein is from about 40% to about 80%, preferably from about 55% to about 75% by weight. It will be appreciated that the specific amount of molasses used may be varied to obtain the desired optimum composition.

Cane raw sugar is a sugar product that is unrefined and contains residual molasses as well as other natural impurities. Although not explicitly understood, the presence of raw sugars in the fermentation reaction contains residual chemicals used in decolorization and final purification and refining that can have some adverse effect on yeast and malt enzymes. It has been found that various properties are greatly improved compared to the case of using sugar. It has been found that optimal biological and enzymatic properties of the fermentation media of the present disclosure are enhanced when raw sugars are included as part of the fermentation feedstock present in the mixture. The amount of cane raw sugar useful in preparing the fermentation media disclosed herein can be from about 10% to about 40%, preferably from about 10% to about 30% by weight. It will be appreciated that the specific amount of cane sugar used may be varied to obtain the desired optimum composition.

Essential enzymes that beneficially contribute to fermentation reactions are obtained by malt and yeast and/or bacteria. The particular malt used is preferably a saccharifying malt containing enzymes including diastase, maltase and amylase. Malt is also believed to enhance yeast and/or bacterial activity, in addition to contributing to the potency and activity of the overall enzyme composition in the final product mixture. The amount of malt useful in preparing the fermentation media disclosed herein can be from about 3% to about 15%, preferably from about 7% to about 12% by weight. It will be appreciated that the specific amount of malt used may be varied to obtain the desired optimum composition.

Fermentation is a metabolic process that breaks down carbohydrates and other complex organic substances into simple substances such as sugars, acids, gases or alcohols. Fermentation occurs in yeasts, bacteria and molds. Fermentation includes ethanol fermentation and lactic acid fermentation. Lactic acid fermentation includes homolactic fermentation and heterolactic fermentation.

Yeast refers to any fermenting fungus that is capable of producing the enzymes necessary for fermentation reactions that convert, for example, carbohydrates into carbon dioxide and alcohol. There are several enzymes produced by active yeast during the fermentation reaction, including both hydrolases and oxidases such as invertase, catalase, lactase, maltase, carboxylase, and the like. Yeasts include fermentation in food processing such as soybean fermentation, dough fermentation, grain fermentation, vegetable fermentation, fruit fermentation, honey fermentation, dairy fermentation, fish fermentation, meat fermentation and tea fermentation. There are useful yeast strains such as A non-exhaustive list of specific yeast genera useful in the fermentation reactions disclosed herein include, but are not limited to, Brettanomyces, Candida, Cyberlindnera, Cystophyllobacillus Cystofilobasidium, Debaryomyces, Dekkera, Fusarium, Geotrichum, Issatchenkia, Kazachstania, Kloekera, Kloekera Genus Kluyveromyces, Lecanicillium, Mucor, Neurospora, Pediococcus, Penicillium, Pichia, Rhizopus, Rhodosporidium, Rhodotorula, Saccharomyces, Schizosaccharomyces, Thrichosporon, Torulaspora, Torulopsis, Torulopsis Included are the genera Verticillium, Yarrowia, Zygosaccharomyces and Zygotorulaspora. A non-exhaustive list of specific yeast species useful in the fermentation reactions disclosed herein includes, but is not limited to, B. B. anomalus, B. B. bruxellensis, B. B. claussenii, B. B. custersianus, B. B. naardenensis, B. B. nanus, C. C. colliculosa, C. C. exiguous, C. C. humicola, C. Kefir (C. kefyr), C. C. krusei, C. C. milleri, C. C. mycoderma, C. C. pelliculosa, C. C. rugose, C. C. stellate, C. C. tropicalis, C. C. utilis, C. C. valida, C. C. vini, C. C. zeylanoides, Cb. mrakii, Cs. Cs. infirmominiatum, D. D. hansenii, D. D. kloeckeri, Dk. anomala, Dk. Dk. bruxellensis, F. F. domesticum, G. G. candidum, I. Orientalis (I. orientalis), K. K. exigua, K. K. unispora, Kl. Kl. africana, Kl. Kl. apis, Kl. Javanica (Kl. javanica), Ku. Ku. lactis, Ku. Ku. marxianus, Ku. Ku. marxianus, L. L. lecanii, M. M. hiemalis, M. M. plumbeus, M. racemosus, M. M. racemosus, N. Intel media (N. intermedia), P. P. cerevisiae, Pn. album (Pn. album), Pn. Pn. camemberti, Pn. caseifulvum, Pn. Chrysogenum (Pn. chrysogenum), Pn. Commune (Pn. commune), Pn. nargiovense, Pn. Pn. roqueforti, Pn. solitum (Pn. solitum), Pi. Pi.fermentans, R.I. R. microspores, Rs. infirmominiatum, Rt. glutinis, Rt. Minuta, Rt. Rt. rubra, S.M. S. bayanus, S. S. boulardii, S. S. carlsbergensis, S. S. cerevisiae, S. cerevisiae; S. eubayanus, S. Paradoxus, S. paradoxus. S. pastorianus, S. S. rouzii, S. S. uvarum, Sc. Pombe (Sc. pombe), Th. Beigelii, T. T. delbrueckii, T. T. franciscae, T. T. pretoriensis, T. T. microellipsoides, T. T. globosa, T. Indica (T. indica), T. indica T. maleeae, T. T. quercuum, To. To. versatilis, V. V. lecanii, Y. Y. lipolytica, Z. lipolytica; Z. bailii, Z. Z. bisporus, Z. bisporus; Z. cidri, Z. Z. fermentati, Z. Z. florentinus, Z. kombuchaensis, Z. kombuchaensis, Z. kombuchaensis; Z. lentus, Z. lentus. Z. mellis, Z. Z. microellipsoides, Z. Z. mrakii, Z. Z. pseudorouxii and Z. pseudorouxii. Z. rouxii and Zt. florentina (Zt. florentina). A preferred yeast is Saccharomyces cerevisiae, commonly available as baker's yeast.

A bacterium refers to any fermenting bacterium capable of producing the enzymes necessary for a fermentation reaction in which an alcohol such as ethanol or an acid such as acetic acid, lactic acid and/or succinic acid is produced. A non-exhaustive list of specific bacterial genera useful in the fermentation reactions disclosed herein include, but are not limited to, Acetobacter, Arthrobacter, Aerococcus , Bacillus, Bifidobacterium, Brachybacterium, Brevibacterium, Barnobacterium, Carnobacterium, Coryne Bacterium (Corynebacterium), Enterococcus, Escherichia, Gluconacetobacter, Gluconobacter, Hafnia, Halomonas, Cochlea ( Kocuria), Lactobacillus, Lactococcus, Leuconostoc, Macrococcus, Microbacterium, Micrococcus, Neisseria ( Neisseria, Oenococcus, Pediococcus, Propionibacterium, Proteus, Pseudomonas, Psychobacter, Salmonella , Sporolactobacillus, Staphylococcus, Streptococcus, Streptomyces, Tetragenococcus, Vagococcus (Vagococcus), Weissels and Zymomonas. A non-exhaustive list of specific bacterial species useful in the fermentation reactions disclosed herein includes, but is not limited to, A. A. aceti, A. A. fabarum, A. A. lovaniensis, A. A. malorum, A. A. orientalis, A. A. pasteurianus, A. A. pasteurianus, A. A. pomorum, A. pomorum. A. syzygii, A. A. tropicalis, Ar. Ar. arilaitensis, Ar. Ar. bergerei, Ar. globiformis, Ar. Ar. nicotianae, Ar. Ar. variabilis, B. B. cereus, B. cereus B. coagulans, B. licheniformis (B. licheniformis), B. B. pumilus, B. pumilus. B. sphaericus, B. B. stearothermophilus, B. B. subtilis, B. B. adolescentis, B. B. animalis, B. B. bifidum, B. B. breve, B. B. infantis, B. B. lactis, B. lactis; B. longum, B. B. pseudolongum, B. B. thermophilum, Br. Br.alimentarium, Br. Br.alimentarium, Br. Br. tyrofermentans, Br. Br. tyrofermentans, Bv. Bv. aurantiacum, Bv. Bv. casei, Bv. Bv. linens, C.I. C. divergens, C. C. maltaromaticum, C. C. piscicola, C. C. ammoniagenes, Co. Co. casei, Co. Co. flavescens, Co. Co. mooreparkense, Co.; Co. variabile, E.M. E. faecalis, E. faecalis. E. faecium, G. Azotocaptans, G. azotocaptans; G. diazotrophicus, G. G. entanii, G. G. europaeus, G. G. hansenii, G. G. johannae, G.J. G. oboediens, G. G. xylinus, Gl. Gl. oxydans, H. H. alvei, Hl. Hl. elongate, K.; Rhizophila, K. rhizophila. Rhizophila, K. rhizophila. K. variants, K. K. variants, L. L. acetotolerans, L. L. acidifarinae, L. L. acidipiscis, L. L. alimentarius, L. L. brevis, L. brevis. L. bucheri, L. L. cacaonum, L. L. casei, L. L. cellobiosus, L. L. collinoides, L. L. composti, L. L. coryniformis, L. L. crispatus, L. L. curvatus, L. L. delbrueckii, L. L. dextrinicus, L. L. diolivorans, L. diolivorans. L. fabifermentans, L. L. farciminis, L. L. fermentum, L. L. gasseri, L. L. ganensis, L. Hammesii (L.hammesii), L. L. harbinensis, L. L. helveticus, L. L. hilgardii, L. L. homohiochii, L. L. jensenii, L. L. johnsonii, L. L. kefiranofaciens, L. kefiranofaciens. L. kefiri, L. kefiri; Kimchi (L. kimchi), L. kimchi L. kisonensis, L. L. kunkeei, L. kunkeei L. mali, L. L. manihotivorans, L. L. mindensis, L. L. mucosae, L. mucosae; L. nagelii, L. L. namuresis, L. namuresis. L. nantesis, L. L. nodensis, L. L. oeni, L. L. otakiensis, L. L. panis, L. L. parabrevis, L. L. parabuchneri, L. L. paracasei, L. paracasei. L. parakefiri, L. L. paralimentarius, L. L. paraplantarum, L. L. pentosus, L. L. perolens, L. L. plantarum, L. L. pobuzihii, L. pobuzihii. L. pontis, L. L. rapi, L. L. reuteri, L. rhamnosus, L. rhamnosus. L. rossiae, L. L. sakei, L. L. salivarius, L. salivarius. L. sanfranciscensis, L. L. satsumensis, L. L. secaliphilus, L. L. senmaizukii, L. L. siliginis, L. siliginis; L. similis, L. similis. L. spicheri, L. L. suevicus, L. sunkii, L. L. tucciti, L. L. vaccinostercus, L. L. versmoldesis, L. L. yamanashiensis, Lc. Lc.lactis, Lc. Raffinolactis (Lc. raffinolactis), Le. Le. carnosum, Le. Le. citreum, Le. Le. fallax, Le. Le. holzapfelii, Le. Le. inhae, Le. Kimchi (Le. kimchi), Le. Le. lactis, Le. mesenteroides, Le. Le. palmae, Le. Pseudomesenteroides, M. M. caseolyticus, Mb. foliorum, Mb. Mb. gubbeenense, Mc. luteus, Mc. Mc.lylae, P.L. acidilactici, P. acidilactici; P. pentosaceus, P. acidipropionici, P. acidipropionici; freudenreichii, P.; P. jensenii, P. P. thoenii, Pr. vulgaris, Ps. fluorescens, Py. Py. celer, S.; S. carnosus, S. S. condiment, S. S. equiorum, S. S. fleurettii, S. S. piscifermentans, S. S. saphrophyticus, S. S. sciuri, S. S. simulans, S. S. succinus, S. S. vitulinus, S. S. warneri, S.; S. xylosus, St. Cremoris, St. St. gallolyticus, St. St. salivarius, St. thermophiles, St. thermophiles, St. St. griseus, T. halophilus, T. halophilus T. koreensis, W. W. beninensis, W. W. cibaria, W. W. fabaria, W. W. ghanesis, W. W. koreensis, W. W. paramesenteroides, W. W. thailandensis and Z. thailandensis. including Z. mobilis.

Mold refers to any fermenting mold that is capable of producing the enzymes necessary for fermentation reactions in which alcohols such as ethanol or acids such as acetic acid, lactic acid and/or succinic acid are produced. A non-exhaustive list of specific fungal genera useful in the fermentation reactions disclosed herein include, but are not limited to, Aspergillus. A non-exhaustive list of specific fungal species useful in the fermentation reactions disclosed herein includes, but is not limited to, A. A. acidus, A. A. fumigatus, A. fumigatus. A. niger, A. A. oryzae and A. Includes A. sojae.

It will be appreciated that the actual amounts of the various types of enzymes produced will depend on several factors, including the types of molasses and sugars used to prepare the fermentation mixture. Again, however, molasses and raw sugars are believed to provide optimum enzyme yield and activity. In one embodiment, the amount of yeast useful in preparing the fermentation media disclosed herein can be from about 0.2% to about 5%, preferably from about 1% to about 3% by weight. . It will be appreciated that the specific amount of yeast used may be varied to obtain the desired optimum composition.

The presence of small amounts of inorganic catalysts, such as magnesium salts, enhances the activity of enzymes to attack and degrade organic wastes during the fermentation reaction as well as subsequently in the product composition. A preferred magnesium salt is magnesium sulfate. The amount of magnesium salt useful in preparing the fermentation media disclosed herein can be from about 0.1% to about 5%, preferably from about 1% to about 3% by weight. It will be appreciated that the specific amount of magnesium salt used may be varied to obtain the desired optimum composition.

For the preparation of fermented microbial supernatant, molasses, sucrose and magnesium salt are added to an appropriate amount of warm water. The specific amount of water used is not critical, but a suitable amount of water is generally about 2 to about 20 times the total weight of the other ingredients of the fermentation medium used in the fermentation reaction. This amount of water is sufficient to facilitate mixing, activate yeast, bacteria and/or mold, and dissolve other ingredients. Furthermore, the water temperature should not be too high, as heat inactivates the malt and yeast enzymes necessary for fermentation. Thus, for example, water temperatures above about 65°C should be avoided, with preferred temperatures between about 25°C and about 45°C. The use of cold water can unduly slow the fermentation reaction rate and should be avoided if fast reaction rates are desired. After the molasses, sugars and magnesium salts have been effectively mixed and dissolved, the malt and yeast are added and the mixture is agitated and allowed to rest until fermentation is substantially complete. The reaction time can be from about 2 days to about 5 days at a temperature of about 20°C to about 45°C. Completion can be readily verified by checking that the reaction mixture has substantially ceased to foam. At the end of the fermentation reaction, the fermenting microbial culture is centrifuged to remove the "sludge" formed during the fermentation process. The resulting fermentation supernatant (usually about 90% to about 98% by weight) is collected and subjected to further processing.

Fermented microbial supernatants contain bionutrients, minerals and amino acids. Bionutrients are typically present in an amount of about 0.01% to about 1% of the total weight of the fermented microbial supernatant. Individual bionutrients are typically present in an amount from about 0.00001% to about 0.01% of the total weight of the fermented microbial supernatant. Examples of bionutrients include, but are not limited to, biotin, folic acid, glucans such as α-glucan and β-glucan, niacin, insotil, pantothenic acid, pyridoxine, riboflavin and thiamine. In aspects of this embodiment, the fermented microbial supernatant disclosed herein contains, for example, from about 0.00001% to about 0.0011% biotin, from about 0.0006% to about 0.016% folic acid. , about 0.005% to about 15% niacin, about 0.01% to about 1% insotil, about 0.00017% to about 0.017% pantothenic acid, about 0.0006% to about 0.016% pyrodoxine, about 0.002% to about 0.023% riboflavin and about 0.001% to about 0.02% thiamine. In other aspects of this embodiment, the fermented microbial supernatant disclosed herein contains, for example, from about 0.00006% to about 0.0006% biotin, from about 0.001% to about 0.011% folic acid, about 0.01% to about 0.1% niacin, about 0.08% to about 0.18% insotil, about 0.002% to about 0.012% pantothenic acid, about 0 0.001% to about 0.011% pyrodoxine, about 0.007% to about 0.017% riboflavin, and about 0.003% to about 0.013% thiamine. In yet another aspect of this embodiment, the fermented microbial supernatant disclosed herein contains, for example, from about 0.00012% to about 0.0006% biotin, from about 0.001% to about 0.011% folic acid, about 0.01% to about 0.1% niacin, about 0.08% to about 0.18% insotil, about 0.003% to about 0.013% pantothenic acid, about 0.001% to about 0.011% pyrodoxine, about 0.008% to about 0.017% riboflavin, and about 0.003% to about 0.013% thiamine. In yet another aspect of this embodiment, the fermented microbial supernatant disclosed herein contains, for example, from about 0.00009% to about 0.0003% biotin, from about 0.004% to about 0.008% folic acid, about 0.03% to about 0.07% niacin, about 0.11% to about 0.15% insotil, about 0.006% to about 0.01% pantothenic acid, about 0.004% to about 0.008% pyrodoxine, about 0.01% to about 0.014% riboflavin, and about 0.006% to about 0.010% thiamine.

Minerals are typically present in an amount from about 0.1% to about 20% of the total weight of the fermented microbial supernatant. Individual minerals are typically present in an amount from about 0.0001% to about 5% of the total weight of the fermented microbial supernatant. Examples of minerals include, but are not limited to calcium, chromium, copper, iron, magnesium, phosphate, potassium, sodium and zinc. In aspects of this embodiment, the fermentation microbial supernatant disclosed herein contains, for example, from about 0.02% to about 0.3% calcium, from about 0.000002% to about 0.0016% chromium. , about 0.000009% to about 0.0014% copper, about 0.00005% to about 0.02% iron, about 0.001% to about 1.3% magnesium, about 0.2% to about 14% phosphate, about 0.4% to about 16% potassium, about 0.2% to about 15% sodium and about 0.08% to about 13% zinc. In other aspects of this embodiment, the fermented microbial supernatant disclosed herein contains, for example, from about 0.07% to about 0.21% calcium, from about 0.000007% to about 0.0011% Chromium, about 0.00004% to about 0.0009% copper, about 0.0001% to about 0.015% iron, about 0.005% to about 0.9% magnesium, about 0.7% to Contains about 9% phosphate, about 0.9% to about 11% potassium, about 0.7% to about 10% sodium and about 0.3% to about 8% zinc. In yet another aspect of this embodiment, the fermented microbial supernatant disclosed herein contains, for example, from about 0.05% to about 1% calcium, from about 0.0001% to about 0.0009% chromium , about 0.00006% to about 0.0007% copper, about 0.0001% to about 0.013% iron, about 0.005% to about 1% magnesium, about 0.1% to about 7% phosphate, about 0.5% to about 9% potassium, about 0.5% to about 8% sodium and about 0.5% to about 6% zinc. In yet another aspect of this embodiment, the fermented microbial supernatant disclosed herein contains, for example, from about 0.12% to about 0.16% calcium, from about 0.0002% to about 0.0006% chromium, about 0.00009% to about 0.0004% copper, about 0.0006% to about 0.01% iron, about 0.01% to about 0.4% magnesium, about 1% to about 4% phosphate, about 2% to about 6% potassium, about 1% to about 5% sodium and about 0.8% to about 3% zinc.

Amino acids are typically present in an amount from about 20% to about 60% of the total weight of the fermented microbial supernatant. Individual amino acids are typically present in an amount from about 0.1% to about 15% of the total weight of the fermented microbial supernatant. Examples of minerals include, but are not limited to, alanine, arginine, aspartic acid, cysteine, glutamic acid, glycine, lysine, methionine, phenylalanine, proline, serine and threonine. In aspects of this embodiment, the fermented microbial supernatant disclosed herein contains, for example, from about 0.2% to about 16% alanine, from about 0.09% to about 15% arginine, from about 0.09% to about 15% arginine. 4% to about 18% aspartic acid, about 0.003% to about 5% cysteine, about 0.5% to about 20% glutamic acid, about 0.09% to about 15% glycine, about 0.09 % to about 15% lysine, from about 0.002% to about 5% methionine, from about 0.09% to about 15% phenylalanine, from about 0.09% to about 15% proline, from about 0.09% Contains about 15% serine and about 0.09% to about 15% threonine. In other aspects of this embodiment, the fermented microbial supernatant disclosed herein contains, for example, about 0.7% to about 11% alanine, about 0.5% to about 10% arginine, about 0 9% to about 13% aspartic acid, about 0.008% to about 1.2% cysteine, about 1% to about 15% glutamic acid, about 0.5% to about 10% glycine, about 0.9% to about 13% aspartic acid, about 0.008% to about 1.2% cysteine, about 1% to about 15% glutamic acid, 8% to about 12% lysine; about 0.2% to about 1.6% methionine; about 0.5% to about 10% phenylalanine; about 0.5% to about 10% proline; 5% to about 10% serine and about 0.5% to about 10% threonine. In yet another aspect of this embodiment, the fermented microbial supernatant disclosed herein contains, for example, about 0.5% to about 9% alanine, about 0.5% to about 8% arginine, about 1% to about 11% aspartic acid, about 0.01% to about 2% cysteine, about 3% to about 13% glutamic acid, about 0.5% to about 8% glycine, about 1% to about 10% % lysine, about 0.3% to about 3% methionine, about 0.5% to about 7% phenylalanine, about 0.5% to about 7% proline, about 0.5% to about 7% Contains serine and about 0.5% to about 7% threonine. In yet another aspect of this embodiment, the fermented microbial supernatant disclosed herein contains, for example, from about 2% to about 6% alanine, from about 1% to about 5% arginine, from about 4% to about 8% aspartic acid, about 0.03% to about 0.7% cysteine, about 6% to about 10% glutamic acid, about 1% to about 5% glycine, about 3% to about 7% lysine, about 0.7% to about 1.1% methionine, about 1% to about 5% phenylalanine, about 1% to about 5% proline, about 1% to about 5% serine and about 1% to about 5% % threonine.

Aspects herein disclose, in part, a treated fermented microbial supernatant. A treated fermented microbial supernatant is defined as any remaining viable yeast, yeast and malt and any other microbially active enzymes present in the fermented microbial supernatant disclosed herein or enzymes from another source. Fermentation microbial supernatant that has been treated to denature, kill, or otherwise destroy. Non-limiting examples of useful treatment methods include a boiling process using high temperature, an autoclaving process using high temperature and high pressure, an irradiation process by exposing the supernatant to ionizing radiation, or the fermenting microorganisms disclosed herein. denature, kill or otherwise destroy any remaining viable yeast, yeast and malt and any other microbial active enzymes or enzymes from another source present in the supernatant; any sterilization step. Additionally, the above process steps may be used alone or in combination with each other or may include pasteurization steps, chemical sterilization steps, as well as proteins such as enzymes and yeast present in the fermentation supernatants disclosed herein. It can also be used in combination with a sterile filtration process that denatures, kills or otherwise destroys microorganisms such as bacteria and/or molds. All of the above methods are routinely used in the field of food preparation and/or sterilization and are processes known to those skilled in the art.

The treated fermented microbial supernatant can then be stored in liquid form for later use. Alternatively, the treated fermented microbial supernatant can be spray dried by methods known in the art to produce a dry powder. A dry powder form can also be stored and then used.

The amount of treated fermented microbial supernatant disclosed herein can be any amount for the disclosed plants, so long as it is useful for practicing the methods and uses disclosed herein. It can be used in pharmaceutical compositions. Factors used to determine the appropriate amount include, for example, whether the treated fermentative microbial supernatant is in liquid or powder form, the specific commercial source of the treated fermentative microbial supernatant, The specific method used to prepare the treated fermented microbial supernatant, whether the botanical drug composition is manufactured as a concentrate or as a ready-to-use product, and whether the botanical drug composition is manufactured as a concentrate. Dilution times desired when preparing from. Generally, there is a greater need for the treated fermented microbial supernatant in liquid form than in dry powder form.

In aspects of this embodiment, the amount of treated fermented microbial supernatant used is, for example, about 0.5 wt%, about 1.0 wt%, about 1.5 wt%, about 2.0 wt%, about 2.5 wt%, about 3.0 wt%, about 3.5 wt%, about 4.0 wt%, about 4.5 wt%, about 5.0 wt%, about 6.0 wt%, about 7.0 wt%, about 7.5 wt%, about 8.0 wt%, about 9.0 wt%, or about 10.0 wt%. In other aspects of this embodiment, the amount of treated fermented microbial supernatant used is, for example, at least 0.5 wt%, at least 1.0 wt%, at least 1.5 wt%, at least 2.0 wt% , at least 2.5 wt%, at least 3.0 wt%, at least 3.5 wt%, at least 4.0 wt%, at least 4.5 wt%, at least 5.0 wt%, at least 6.0 wt%, at least 7.0 wt%, at least 7.5 wt%, at least 8.0 wt%, at least 9.0 wt%, or at least 10.0 wt%. In yet another aspect of this embodiment, the amount of treated fermented microbial supernatant used is e.g. %, max 2.5 wt%, max 3.0 wt%, max 3.5 wt%, max 4.0 wt%, max 4.5 wt%, max 5.0 wt%, max 6.0 wt% , up to 7.0% by weight, up to 7.5% by weight, up to 8.0% by weight, up to 9.0% by weight or up to 10.0% by weight. In yet another aspect of this embodiment, the amount of treated fermented microbial supernatant used is, for example, from about 0.1% to about 2.5%, from about 0.1% to about 3.0% by weight. %, about 0.1 wt% to about 3.5 wt%, about 0.1 wt% to about 4.0 wt%, about 0.1 wt% to about 5.0 wt%, about 0.5 wt% to about 2.5 wt%, from about 0.5 wt% to about 3.0 wt%, from about 0.5 wt% to about 3.5 wt%, from about 0.5 wt% to about 4.0 wt%, about 0.5 wt% to about 5.0 wt%, about 1 wt% to about 2.5 wt%, about 1 wt% to about 3.0 wt%, about 1 wt% to about 3.5 wt%, about 1 wt% to about 4.0 wt%, about 1 wt% to about 5.0 wt%, about 1 wt% to about 6.0 wt%, about 1 wt% to about 7.0 wt%, about 1 % to about 8.0%, from about 1% to about 9.0%, or from about 1% to about 10.0% by weight.

In other aspects of this embodiment, the amount of treated fermented microbial supernatant used is, for example, about 15.0 wt%, about 20.0 wt%, about 25.0 wt%, about 30.0 wt% , about 35.0 wt%, about 40.0 wt%, about 45.0 wt%, about 50.0 wt%, about 55.0 wt%, about 60.0 wt%, about 65.0 wt%, about 70.0 wt%, about 75.0 wt%, about 80.0 wt%, about 85.0 wt%, or about 90.0 wt%. In yet another aspect of this embodiment, the amount of treated fermented microbial supernatant used is, for example, at least 15.0 wt%, at least 20.0 wt%, at least 25.0 wt%, at least 30.0 wt% %, at least 35.0 wt%, at least 40.0 wt%, at least 45.0 wt%, at least 50.0 wt%, at least 55.0 wt%, at least 60.0 wt%, at least 65.0 wt% , at least 70.0% by weight, at least 75.0% by weight, at least 80.0% by weight, at least 85.0% by weight, or at least 90.0% by weight. In yet another aspect of this embodiment, the amount of treated fermented microbial supernatant used is, for example, up to 15.0% by weight, up to 20.0% by weight, up to 25.0% by weight, up to 30.0% by weight %, max 35.0 wt%, max 40.0 wt%, max 45.0 wt%, max 50.0 wt%, max 55.0 wt%, max 60.0 wt%, max 65.0 wt% , up to 70.0% by weight, up to 75.0% by weight, up to 80.0% by weight, up to 85.0% by weight, or up to 90.0% by weight.

In other aspects of this embodiment, the amount of treated fermented microbial supernatant used is, for example, from about 5% to about 7.5%, from about 5% to about 10%, from about 5% to about 15 wt%, about 5 wt% to about 20 wt%, about 5 wt% to about 25 wt%, about 5 wt% to about 30 wt%, about 5 wt% to about 35 wt%, about 5 wt% to about 40 wt%, about 5 wt% to about 45 wt%, about 5 wt% to about 50 wt%, about 5 wt% to about 55 wt%, about 5 wt% to about 60 wt%, about 5 wt% to about 65 wt%, about 5 wt% to about 70 wt%, about 5 wt% to about 75 wt%, about 5 wt% to about 80 wt%, about 5 wt% to about 85 wt%, about 5 wt% to about 90 wt%, about 5 wt% to about 95 wt%, about 10 wt% to about 15 wt%, about 10 wt% to about 20 wt%, about 10 wt% to about 25 wt%, about 10 wt% to about 30 wt%, about 10 wt% to about 35 wt%, about 10 wt% to about 40 wt%, about 10 wt% to about 45 wt%, about 10 wt% to about 50 wt%, about 10 wt% to about 55 wt%, about 10 wt% to about 60 wt%, about 10 wt% to about 65 wt%, about 10 wt% to about 70 wt%, about 10 wt% to about 75 wt%, about 10 wt% to about 80 wt%, about 10 wt% to about 85 wt%, about 10 wt% to about 90 wt%, about 10 wt% to about 95 wt%, about 15 wt% to about 20 wt%, about 15 wt% to about 25 wt%, about 15 wt% to about 30 wt%, about 15 wt% to about 35 wt%, about 15 wt% to about 40 wt%, about 15 wt% to about 45 wt%, about 15 wt% to about 50 wt%, about 15 wt% to about 55 wt%, about 15 wt% to about 60 wt%, about 15 wt% to about 65 wt%, about 15 wt% to about 70 wt%, about 15 wt% to about 75 wt%, about 15 wt% to about 80 wt%, about 15 wt% to about 85 wt%, about 15 wt% to about 90 wt%, about 15 wt% to about 95 wt%, about 25 wt% to about 25 wt%, about 25 wt% to about 30 wt%, about 25 wt% to about 35 wt%, about 25 wt% to about 40 wt%, about 25 wt% to about 45 wt%, about 25 wt% to about 50 wt%, about 25 wt% to about 55 wt%, about 25 wt% to about 60 wt%, about 25 wt% to about 65 wt%, about 25 wt% to about 70 wt%, about 25 wt% to about 75 wt%, about 25 wt% to about 80 wt%, about 25 wt% to about 85 wt%, about 25 wt% to about 90 wt%, about 25 wt% to about 95 wt%, about 25 wt% to about 30% by weight, about 25% to about 35% by weight, about 25% to about 40% by weight, about 25% to about 45%, about 25% to about 50%, about 25% to about 55%, about 25% to about 60%, about 25% to about 65%, about 25% to about 70%, about 25% to about 75%, about 25% to about 80%, about 25% to about 85%, about 25% to about 90%, about 25% to about 95%, about 30% to about 35%, about 30% to about 40%, about 30% to about 45%, about 30% to about 50%, about 30% to about 55%, about 30% to about 60%, about 30% to about 65%, about 30% to about 70%, about 30% to about 75%, about 30% to about 80%, about 30% to about 85%, about 30% to about 90%, about 30% to about 95%, about 35% to about 40%, about 35% to about 45%, about 35% to about 50%, about 35% to about 55%, about 35% to about 60%, about 35% to about 65%, about 35% to about 70%, about 35% to about 75%, about 35% to about 80%, about 35% to about 85%, about 35% to about 90%, about 35% to about 95%, about 40% to about 45%, about 40% to about 50%, about 40% to about 55%, about 40% to about 60%, about 40% to about 65%, about 40% to about 70%, about 40% to about 75%, about 40% to about 80%, about 40% to about 85%, about 40% to about 90%, about 40% to about 95%, about 45% to about 50%, about 45% to about 55%, about 45% to about 60%, about 45% to about 65%, about 45% to about 70%, about 45% to about 75%, about 45% to about 80%, about 45% to about 85%, about 45% to about 90%, about 45% to about 95%, about 50% to about 55%, about 50% to about 60%, about 50% to about 65%, about 50% to about 70%, about 50% to about 75%, about 50% to about 80%, about 50% to about 85%, about 50% to about 90%, about 50% to about 95%, about 55% to about 60%, about 55% to about 65%, about 55% to about 70%, about 55% to about 75%, about 55 wt% to about 80 wt%, about 55 wt% to about 85 wt%, about 55 wt% to about 90 wt%, about 55 wt% to about 95 wt%, about 60 wt% to about 65 wt%, about 60 wt% to about 70 wt%, about 60 wt% to about 75 wt%, about 60 wt% to about 80 wt%, about 60 wt% to about 85 wt%, about 60 wt% to about 90 wt%, about 60 wt% to about 95 wt%, about 65 wt% to about 70 wt%, about 65 wt% to about 75 wt%, about 65 wt% to about 80 wt%, about 65 wt% to about 85 wt%, about 65 wt% to about 90 wt%, about 65 wt% to about 95 wt%, about 70 wt% to about 75 wt%, about 70 wt% to about 80 wt%, about 70 wt% to about 85 wt%, about 70 wt% to about 90 wt%, about 70 wt% to about 95 wt%, about 75 wt% to about 80 wt%, about 75 wt% to about 85 wt%, about 75 wt% to about 90 wt%, about 75 wt% to about 95 wt%, about 80 wt% to about 85 wt%, about 80 wt% to about 90 wt%, about 80 wt% to about 95 wt%, about 85 wt% to about 90 wt%, about 85 wt% to about 95 wt%, or about 90 wt% to about 95% by weight.

Aspects herein disclose, in part, surfactants. A surfactant is a compound that lowers the surface tension of a liquid to facilitate diffusion and lowers the interfacial tension between two liquids or between a liquid and a solid. A single surfactant may be mixed with the buffer solutions disclosed herein, or multiple surfactants may be mixed with the buffer solutions disclosed herein. Useful surfactants include, but are not limited to, ionic surfactants, zwitterionic (amphoteric) surfactants, nonionic surfactants or any combination thereof. Detergents used in the methods disclosed herein can be varied as needed by one skilled in the art and generally depend in part on the particular buffer used, the protein to be eluted and the conductivity value used. dependent on the

Ionic surfactants include anionic surfactants. Anionic surfactants include those based on durable functional groups attached to the head, such as sulfate, sulfonate, phosphate, carboxylate groups, etc., or pH-dependent anionic surfactants. Anionic surfactants include, but are not limited to, alkyl sulfates such as ammonium lauryl sulfate and sodium lauryl sulfate (SDS); alkyl ether sulfates such as sodium laureth sulfate and sodium myreth sulfate; docusates such as dioctyl sodium sulfosuccinate; sulfonate fluorosurfactants such as sulfonates (PFOS) and perfluorobutanesulfonate; DOWFAX™ 2A1 (disodium lauryl phenyl ether disulfonate), DOWFAX™ 3B2 (disodium decylphenyl ether disulfonate), Alkyl diphenyl oxide disulfonates such as DOWFAX™ C10L (disodium decylphenyl ether disulfonate), DOWFAX™ 2EP and DOWFAX™ 8390 (disodium cetylphenyl ether disulfonate); TRITON™ H- potassium phosphate polyether esters such as 55 and TRITON™ H-66; alkyl benzene sulfonates; alkyl aryl ether phosphates; alkyl ether phosphates; fatty acid salts and alkyl carboxylates such as sodium stearate; carboxylic acid fluorosurfactants such as perfluorononanoate and perfluorooctanoate; and sodium hexyl diphenyl ether sulfonate (DOWFAX™ C6L).

Ionic surfactants also include cationic surfactants. Cationic surfactants include those based on permanent or pH dependent cationic surfactants such as primary, secondary or tertiary amines. Cationic surfactants include, but are not limited to, alkyltrimethylammonium salts such as cetyltrimethylammonium bromide (CTAB) and cetyltrimethylammonium chloride (CTAC); cetylpyridinium chloride (CPC); polyethoxylated tallowamine (POEA); benzethonium chloride (BZT); 5-bromo-5-nitro-1,3-dioxane; dimethyldioctadecylammonium chloride; and dioctadecyldimethylammonium bromide (DODAB), as well as octenidine pH-dependent primary, secondary or tertiary amines such as surfactants that positively charge the primary amine above pH 10 or charge the secondary amine below pH 4, such as dihydrochloride are mentioned. Other useful anionic surfactants include, but are not limited to, bio-anionic surfactants, such as STEPONOL® AM30-KE of ammonium lauryl sulfate and STEPONOL® EHS of sodium 2-ethylhexyl sulfate. is included in this. Such biosurfactants are not synthetic molecules, but anionic surfactants derived from organic matter such as plants.

Zwitterionic surfactants are based on primary, secondary or tertiary amines or quaternary ammonium cations with sulphonates, carboxylates or phosphates. Zwitterionic surfactants include, but are not limited to, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS); sultaines such as cocamidopropyl hydroxysultaine; cocamide; Betaines such as propyl betaine; or lecithin.

Nonionic detergents are less denaturing than others and are useful for solubilizing membrane proteins and lipids while preserving protein-protein interactions. Nonionic surfactants include polyether nonionic surfactants, polyhydroxyl nonionic surfactants and biosurfactants. Nonionic surfactants include alcohol ethoxylates, alkylphenol ethoxylates, phenol ethoxylates, amide ethoxylates, glyceride ethoxylates, fatty acid ethoxylates and fatty amine ethoxylates. ^The nonionic surfactants disclosed herein have the general _formula H _{( OCH2CH2} ) _xOC6H4R1 , H ⁽ _{OCH2CH2 ) xOR2} or H _{( OCH2CH2 ) x} OC(O) ^R2 , where x represents the number of moles of ethylene oxide attached to the alkylphenol and/or fatty alcohol or fatty acid, ^R1 represents a long-chain alkyl group, and ^R2 is represents a long-chain aliphatic group. In aspects of this embodiment, R ¹ is a C ₇ -C ₁₀ alkyl group and/or R ² is a C ₁₂ -C ₂₀ aliphatic group. Other useful nonionic surfactants include, but are not limited to, bio-nonionic surfactants including, but not limited to, STEPOSOL® MET-10U, a nonionic surfactant that is an unsaturated short-chain amide obtained by metathesis. agents. Such biosurfactants are non-ionic biosurfactants derived from organic matter such as plants, rather than synthetic molecules.

Non-limiting examples of surfactants include polysorbate 20 sorbitan monooleate (TWEEN® 20), polysorbate 40 sorbitan monooleate (TWEEN® 40), polysorbate 60 sorbitan monooleate (TWEEN® 40). ® 60), polysorbate 61 sorbitan monooleate (TWEEN® 61), polysorbate 65 sorbitan monooleate (TWEEN® 65), polysorbate 80 sorbitan monooleate (TWEEN® 80) ), polyoxyethylene glycol sorbitan alkyl esters (or ethoxylated sorbital esters) such as polysorbate 81 sorbitan monooleate (TWEEN® 81) and polysorbate 85 sorbitan monooleate (TWEEN® 85); sorbitan Sorbital esters such as monooleate, sorbitan monolaurate, sorbitan monopalmitate, mosorbitan monostearate and sorbitan tristearate; glycerol monooleate, glycerol monolaurate, glycerol monopalmitate, glycerol monostearate, glycerol Polyglycerol esters such as trioleate, glycerol ricinoleate, glycerol tristearate, monodiglycerides and glycerol triacetate; ethoxylated polyglycerol esters; arachidyl glucoside, _C12-20 alkyl glucoside, caprylyl/capryl glucoside, cetearyl glucoside , coco-glucoside, ethyl glucoside and lauryl glucoside, decyl glucoside; ethoxylated alkyl glucosides; ethoxylated sucrose esters; amine oxides; ethoxylated alcohols; ethoxylated fatty alcohols; alkylamines; ethoxylated alkylamines; ethoxylated alkylphenols such as octylphenol; alkyl polysaccharides; ethoxylated alkyl ethoxylated fatty acids such as ethoxylated castor oil; ethoxylated fatty alcohols such as ethoxylated ceto-oleyl alcohol, ethoxylated ceto-stearyl alcohol, ethoxylated decyl alcohol, ethoxylated dodecyl alcohol and ethoxylated tridecyl alcohol; Ethoxylated Fatty Amines; Poloxamer 124 (Pluronic L44), Poloxamer 181 (Pluronic L61), Poloxamer 182 (Pluronic L62), Poloxamer 184 (Pluronic L64), Poloxamer Poloxamer (polyethylene-polypropylene copolymer ); TERGITOL™ 15-S-5, TERGITOL™ 15-S-7, TERGITOL™ 15-S-9, TERGITOL™ 15-S-12, TERGITOL™ 15-S- 15, linear secondary alcohol ethoxylates such as TERGITOL™ 15-S-20, TERGITOL™ 15-S-30 and TERGITOL™ 15-S-40; alkylphenol polyglycol ethers; polyethylene glycol alkyls aryl ethers; polyoxyethylene glycol alkyl ethers such as octaethylene glycol monododecyl ether, pentaethylene glycol monododecyl ether, BRIJ® 30 and BRIJ® 35; 2-dodecoxyethanol (LUBROL® )-PX); polyoxyethylenes such as polyoxyethylene (4-5)pt-octylphenol (TRITON® X-45) and polyoxyethylene octylphenyl ether (TRITON® X-100); glycol octylphenol ethers; polyoxyethylene glycol alkylphenol ethers such as nonoxynol-9; ethano glucoside alkyl ethers such as octylglucopyranoside; maltoside alkyl ethers such as dodecyl maltopyranoside; thioglucoside alkyl ethers such as heptylthioglucopyranoside; digitonin; glycerol alkyl esters such as glyceryl laurate; sulfonic acid alcohols; sorbitan alkyl esters; cocamide ethanolamines such as cocamide monoethanolamine and cocamide diethanolamine; sucrose monolaurate; dodecyldimethylamine oxide and sodium cholate. Other examples of surfactants useful in the methods disclosed herein are found, for example, in Winslow et al., Methods and Compositions for Simultaneously Isolating Hemoglobin from Red Blood Cells and Inactivating Viruses, U.S. Patent Application Publication No. 2008/0138790; Ｐｈａｒｍａｃｅｕｔｉｃａｌ Ｄｏｓａｇｅ Ｆｏｒｍｓ ａｎｄ Ｄｒｕｇ Ｄｒｕｇ Ｄｅｌｉｖｅｒｙ Ｓｙｓｔｅｍｓ（Ｈｏｗａｒｄ Ｃ．Ａｎｓｅｌら編，Ｌｉｐｐｉｎｃｏｔｔ Ｗｉｌｌｉａｍｓ ＆ Ｗｉｌｋｉｎｓ Ｐｕｂｌｉｓｈｅｒｓ，第７版，１９９９）；Ｒｅｍｉｎｇｔｏｎ：Ｔｈｅ Ｓｃｉｅｎｃｅ ａｎｄ Ｐｒａｃｔｉｃｅ ｏｆ Ｐｈａｒｍａｃｙ（Ａｌｆｏｎｓｏ Ｒ．Ｇｅｎｎａｒｏら，Ｌｉｐｐｉｎｃｏｔｔ，Ｗｉｌｌｉａｍｓ ＆ Ｗｉｌｋｉｎｓ，第２０版，２０００）；Ｇｏｏｄｍａｎ ＆ Ｇｉｌｍａｎ'ｓ Ｔｈｅ Ｐｈａｒｍａｃｅｕｔｉｃａｌ Ｂａｓｉｓ ｏｆ Ｔｈｅｒａｐｅｕｔｉｃｓ（Ｊｏｅｌ Ｇ．Ｈａｒｄｍａｎら編，ＭｃＧｒａｗ－Ｈｉｌｌ Ｐｒｏｆｅｓｓｉｏｎａｌ，第１０版，２００１）；およびＨａｎｄｂｏｏｋ ｏｆ Ｐｈａｒｍａｃｅｕｔｉｃａｌ Ｅｘｃｅｐｉｅｎｔｓ（Ｒａｙｍｏｎｄ Ｃ．Ｒｏｗｅら，ＡＰｈＡ Publications, 4th Edition, 2003), each of which is incorporated herein by reference in its entirety.

Nonionic surfactants act synergistically to enhance the action of the fermented microbial supernatant. Additionally, the nonionic surfactants used in the botanical pharmaceutical compositions disclosed herein have been found to be compatible with promoting chemical reactions. Accordingly, in one embodiment, the botanical pharmaceutical compositions disclosed herein contain only one or more nonionic surfactants. In another embodiment, the botanical pharmaceutical compositions disclosed herein contain only one or more nonionic surfactants and one or more anionic surfactants. In another embodiment, the botanical pharmaceutical compositions disclosed herein do not contain any cationic surfactants. In another embodiment, the botanical pharmaceutical compositions disclosed herein do not contain cationic or zwitterionic surfactants. In another embodiment, the botanical pharmaceutical compositions disclosed herein do not contain any ionic surfactants. In another embodiment, the botanical pharmaceutical compositions disclosed herein do not contain any ionic or zwitterionic surfactants.

Any amount of surfactant disclosed herein can be used as long as it is useful for carrying out the methods disclosed herein. In aspects of this embodiment, the amount of surfactant used is, for example, about 0.01 wt.%, about 0.05 wt.%, about 0.075 wt.%, about 0.1 wt.%, about 0.05 wt. 2 wt%, about 0.3 wt%, about 0.4 wt%, about 0.5 wt%, about 0.6 wt%, about 0.7 wt%, about 0.8 wt%, about 0.9 wt% % by weight, about 1.0% by weight, about 1.5% by weight, about 2.0% by weight, about 2.5% by weight, about 3.0% by weight, about 4.0% by weight, about 5.0% by weight %, about 6.0 wt%, about 7.0 wt%, about 7.5 wt%, about 8.0 wt%, about 9.0 wt%, or about 10.0 wt%. In other aspects of this embodiment, the amount of surfactant used is, for example, at least 0.01 wt%, at least 0.05 wt%, at least 0.075 wt%, at least 0.1 wt%, at least 0 .25% by weight, at least 0.5% by weight, at least 0.75% by weight, at least 1.0% by weight, at least 1.5% by weight, at least 2.0% by weight, at least 2.5% by weight, at least 3.0% by weight; 0 wt%, at least 4.0 wt%, at least 5.0 wt%, at least 6.0 wt%, at least 7.0 wt%, at least 7.5 wt%, at least 8.0 wt%, at least 9.0 wt% % by weight or at least 10.0% by weight. In yet another aspect of this embodiment, the amount of surfactant used is, for example, up to 0.01 wt%, up to 0.05 wt%, up to 0.075 wt%, up to 0.1 wt%, up to 0.25 wt%, max 0.5 wt%, max 0.75 wt%, max 1.0 wt%, max 1.5 wt%, max 2.0 wt%, max 2.5 wt%, max 3 .0% by weight, up to 4.0% by weight, up to 5.0% by weight, up to 6.0% by weight, up to 7.5% by weight, up to 8.0% by weight, up to 9.0% by weight or up to 10.0% by weight. 0% by weight.

In yet another aspect of this embodiment, the amount of surfactant used is, for example, from about 0.1% to about 0.5%, from about 0.1% to about 0.75%, from about 0.1 wt% to about 1.0 wt%, about 0.1 wt% to about 1.5 wt%, about 0.1 wt% to about 2.0 wt%, about 0.1 wt% to about 2 .5% by weight, from about 0.2% to about 0.5%, from about 0.2% to about 0.75%, from about 0.2% to about 1.0%, from about 0.5% by weight; 2 wt% to about 1.5 wt%, about 0.2 wt% to about 2.0 wt%, about 0.2 wt% to about 2.5 wt%, about 0.5 wt% to about 1.0 wt% % by weight, about 0.5% to about 1.5%, about 0.5% to about 2.0%, about 0.5% to about 2.5%, about 0.5% by weight % to about 3.0 wt%, about 0.5 wt% to about 4.0 wt%, about 0.5 wt% to about 5.0 wt%, about 1.0 wt% to about 2.5 wt% , about 1.0% to about 3.0%, about 1.0% to about 4.0%, about 1.0% to about 5.0%, about 1.0% to about 6.0 wt%, about 1.0 wt% to about 7.0 wt%, about 1.0 wt% to about 7.5 wt%, about 1.0 wt% to about 8.0 wt%, about 1.0 wt% to about 9.0 wt%, about 1.0 wt% to about 10.0 wt%, about 2.0 wt% to about 2.5 wt%, about 2.0 wt% to about 3 0 wt%, about 2.0 wt% to about 4.0 wt%, about 2.0 wt% to about 5.0 wt%, about 2.0 wt% to about 6.0 wt%, about 2.0 wt% 0 wt% to about 7.0 wt%, about 2.0 wt% to about 7.5 wt%, about 2.0 wt% to about 8.0 wt%, about 2.0 wt% to about 9.0 wt% % by weight, about 2.0% to about 10.0%, about 5.0% to about 6.0%, about 5.0% to about 7.0%, about 5.0% by weight % to about 7.5 wt%, about 5.0 wt% to about 8.0 wt%, about 5.0 wt% to about 9.0 wt%, about 5.0 wt% to about 10.0 wt% , about 5.0% to about 11.0%, about 5.0% to about 12.0%, about 5.0% to about 13.0%, about 5.0% to about 14.0 wt%, or about 5.0 wt% to about 15.0 wt%.

Aspects herein disclose, in part, the pH of the botanical pharmaceutical compositions disclosed herein. The final pH of a botanical drug composition is usually acidic as it contributes to prolonging the shelf life of the composition. In aspects of this embodiment, the pH of the botanical compositions disclosed herein is, for example, about 2, about 2.5, about 3, about 3.5, about 4, about 4.5, about 5, about 5.5 or about 6. In other aspects of this embodiment, the pH of the botanical compositions disclosed herein is, for example, at least 2, at least 2.5, at least 3, at least 3.5, at least 4, at least 4.5 , at least 5, at least 5.5 or at least 6. In yet another aspect of this embodiment, the pH of the botanical compositions disclosed herein is, for example, up to 2, up to 2.5, up to 3, up to 3.5, up to 4, up to 4.5. 5, up to 5, up to 5.5 or up to 6. In yet another aspect of this embodiment, the pH of the botanical pharmaceutical compositions disclosed herein is, for example, about 2 to about 3, about 2 to about 3.5, about 2 to about 4, about 2 to about 4.5, about 2 to about 5, about 2 to about 5.5, about 2 to about 6, about 2.5 to about 3, about 2.5 to about 3.5, about 2.5 to about 4, about 2.5 to about 4.5, about 2.5 to about 5, about 2.5 to about 5.5, about 2.5 to about 6, about 3 to about 3.5, about 3 to about 4, about 3 to about 4.2, about 3 to about 4.5, about 3 to about 4.7, about 3 to about 5, about 3 to about 5.2, about 3 to about 5.5, about 3 to about 6, about 3.5 to about 4, about 3.5 to about 4.2, about 3.5 to about 4.5, about 3.5 to about 4.7, about 3.5 to about 5, about 3.5 to about 5.2, about 3.5 to about 5.5, about 3.5 to about 6, about 3.7 to about 4.0, about 3.7 to about 4.2, about 3 .7 to about 4.5, about 3.7 to about 5.2, about 3.7 to about 5.5 or about 3.7 to about 6.0.

The botanical pharmaceutical compositions disclosed herein have minimal adverse effects on humans, mammals including livestock, plants and the environment. In one aspect of this embodiment, the botanical compositions disclosed herein are substantially non-toxic to humans, mammals, plants and the environment. In another aspect of this embodiment, the botanical pharmaceutical compositions disclosed herein are essentially non-toxic to humans, mammals, plants and the environment.

Aspects herein disclose partially biodegradable botanical pharmaceutical compositions. The biodegradable botanical compositions disclosed herein are those that tend to undergo a significant degree of degradation, decay, absorption, putrefaction or destruction after being used in accordance with the methods and uses disclosed herein. Composition. In aspects of this embodiment, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99% of the botanical compositions disclosed herein are, for example, about 1 days, about 2 days, about 3 days, about 4 days, about 5 days, about 6 days or about 7 days. In other aspects of this embodiment, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99% of the botanical compositions disclosed herein are, for example, about 1 to about 2 days, about 1 to about 3 days, about 1 to about 4 days, about 1 to about 5 days, about 1 to about 6 days, about 1 to about 7 days, about 2 to about 3 days, about 2 ~ about 4 days, about 2 to about 5 days, about 2 to about 6 days, about 2 to about 7 days, about 3 to about 4 days, about 3 to about 5 days, about 3 to about 6 days, about 3 ~ Biodegrades in about 7 days, about 4 to about 5 days, about 4 to about 6 days, about 4 to about 7 days, about 5 to about 6 days, about 5 to about 7 days, or about 6 to about 7 days.

In aspects of this embodiment, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99% of the botanical compositions disclosed herein are, for example, about 7 days, about 8 days, about 9 days, about 10 days, about 11 days, about 12 days, about 13 days or about 14 days. In other aspects of this embodiment, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99% of the botanical compositions disclosed herein are, for example, about 7 to about 8 days, about 7 to about 9 days, about 7 to about 10 days, about 7 to about 11 days, about 7 to about 12 days, about 7 to about 13 days, about 7 to about 14 days, about 8 ~ about 9 days, about 8 to about 10 days, about 8 to about 11 days, about 8 to about 12 days, about 8 to about 13 days, about 8 to about 14 days, about 9 to about 10 days, about 9 ~ about 11 days, about 9 to about 12 days, about 9 to about 13 days, about 9 to about 14 days, about 9 to about 11 days, about 9 to about 12 days, about 9 to about 13 days, about 9 to about 14 days, about 10 to about 11 days, about 10 to about 12 days, about 10 to about 13 days, about 10 to about 14 days, about 11 to about 12 days, about 11 to about 13 days, about 11 to about 14 days days, about 12 to about 13 days, about 12 to about 14 days, or about 13 to about 14 days.

In aspects of this embodiment, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99% of the botanical compositions disclosed herein are, for example, about 15% days, about 16 days, about 17 days, about 18 days, about 19 days, about 20 days or about 21 days. In other aspects of this embodiment, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99% of the botanical compositions disclosed herein are, for example, about 15 to about 16 days, about 15 to about 17 days, about 15 to about 18 days, about 15 to about 19 days, about 15 to about 20 days, about 15 to about 21 days, about 16 to about 17 days, about 16 ~ about 18 days, about 16 to about 19 days, about 16 to about 20 days, about 16 to about 21 days, about 17 to about 18 days, about 17 to about 19 days, about 17 to about 20 days, about 17 to about Biodegrades in about 21 days, about 18 to about 19 days, about 18 to about 20 days, about 18 to about 21 days, about 19 to about 20 days, about 19 to about 21 days, or about 20 to about 21 days.

Aspects herein disclose, in part, kits that include one or more components useful for practicing the methods or uses disclosed herein. Kits provide components useful for practicing the methods or uses disclosed herein conveniently packaged to facilitate or facilitate their commercial sale. For example, a kit may include a botanical pharmaceutical composition disclosed herein and one or more other reagents, such as one or more, useful for practicing the methods or uses disclosed herein. diluent and/or one or more carriers and the like.

Kits typically provide one or more components useful for practicing the methods or uses disclosed herein in a suitable container, e.g., a box or other sealed shipping container. do. In addition, kits disclosed herein typically include one or more of the components in separate containers, such as bottles, vials, flasks or other sealed carrier containers. For example, a kit includes a container for a botanical drug composition disclosed herein and separate containers for one or more other reagents. Kits may be portable, eg, capable of being transported and used in remote locations such as commercial facilities or agricultural fields. Alternatively, the kit may be for use in residential buildings.

Kits disclosed herein may include a label or an insert. Labels or inserts include "printed material" that may be provided as a separate material, packaging material (eg, box), or may be attached or affixed to a container containing kit components. The label or insert may be a computer readable medium such as a disc (e.g. hard disk, flash memory), optical disc such as CD-ROM/RAM or DVD-ROM/RAM, DVD, MP3, magnetic tape or RAM and ROM. or hybrids thereof, such as magnetic/optical storage media, flash media or memory type cards. The label or insert may include identification of one or more of the components therein, dosage, frequency or timing of dosage, information regarding individual components. The label or insert may contain information indicating manufacturer information, lot number, manufacturer location and date. The label or insert may contain information regarding the conditions or circumstances under which the kit components may be used. A label or insert can include instructions for using one or more of the kit components in a method or use disclosed herein. The instructions include the amount, frequency or duration of input and instructions for carrying out any of the methods, uses or treatment protocols described herein, as well as any potential hazards or components of the kit. Warnings regarding unsuitable conditions for use may be included.

Aspects herein disclose, in part, methods of controlling pathogens of plant diseases. The disclosed method of controlling a plant disease pathogen comprises the steps of applying an effective amount of a botanical pharmaceutical composition disclosed herein to one or more plants infested with the pathogen and/or Applying an effective amount of a botanical drug composition disclosed herein to one or more loci so as to be exposed to the pharmaceutical composition. Application of the botanical pharmaceutical compositions disclosed herein, for example, results in an adverse effect on the pathogen of the plant disease to be controlled.

Aspects herein disclose, in part, the use of the botanical compositions disclosed herein for controlling pathogens of plant diseases. The use of the disclosed botanical compositions comprises applying an effective amount of the botanical compositions disclosed herein to one or more plants infested with the pathogen and/or This includes applying an effective amount of a botanical drug composition disclosed herein to one or more locations exposed to the composition. Application of the botanical pharmaceutical compositions disclosed herein, for example, results in a detrimental effect on the pathogen of the plant disease to be controlled.

Aspects herein disclose, in part, methods of increasing plant growth and/or crop production. The disclosed methods of increasing plant growth and/or crop production comprise applying to one or more plants an effective amount of a botanical composition disclosed herein and/or applying an effective amount of a botanical drug composition disclosed herein to one or more loci so as to expose one or more plants to the composition. Application of the botanical pharmaceutical compositions disclosed herein results in, for example, improved uptake by root hairs, improved xylem sap flow through the xylem and improved photosynthate flow at the phloem, water from the soil, Increased absorption of minerals and other nutrients, increased capillary action and/or hydrostatic pressure in xylem and/or increased synthesis of compounds and energy and/or inhibition of xylem sap flow and/or photosynthetic product flow1 Destruction of one or more components is provided. In one embodiment, the one or more components that inhibit xylem sap flow and/or photosynthetic product flow include biofilms.

Aspects herein disclose, in part, the use of the botanical compositions disclosed herein for enhancing plant growth and/or crop production. Use of the disclosed botanical compositions involves applying to one or more plants an effective amount of the botanical compositions disclosed herein and/or applying one or more This includes applying an effective amount of a botanical pharmaceutical composition disclosed herein to one or more locations exposing a plurality of plants. The botanical pharmaceutical compositions disclosed herein provide, for example, improved absorption by root hairs, improved xylem sap flow through the xylem and improved photosynthetic product flow at the phloem, water, minerals and increasing absorption of other nutrients, increasing capillary action and/or hydrostatic pressure in xylem and/or increasing synthesis of compounds and energy and/or inhibiting xylem sap flow and/or photosynthetic product flow, or Destruction of multiple components is provided. In one embodiment, the one or more components that inhibit xylem sap flow and/or photosynthetic product flow include biofilms.

Aspects herein disclose, in part, methods of maintaining or improving the efficiency of an irrigation system. A disclosed method of maintaining or improving efficiency of an irrigation system comprises applying an effective amount of a botanical drug composition disclosed herein to one or more pipes in a pipeline network of the irrigation system. include. Application of the botanical pharmaceutical compositions disclosed herein, for example, substantially removes one or more components that interfere with one or more pipeline networks of an irrigation system. In one embodiment, the one or more components that impede the one or more pipeline networks include biofilms.

Aspects herein disclose, in part, the use of the botanical compositions disclosed herein to maintain or improve the efficiency of an irrigation system. Uses of the disclosed botanical compositions include applying an effective amount of the botanical compositions disclosed herein to one or more pipes in the pipeline network of an irrigation system. Application of the botanical pharmaceutical compositions disclosed herein substantially removes, for example, one or more components that interfere with one or more pipeline networks of an irrigation system. In one embodiment, the one or more components that impede the one or more pipeline networks include biofilms.

The combination of nonionic surfactants and treated fermented microbial supernatants contained in the botanical pharmaceutical compositions disclosed herein results in the following: 2) one or more components that inhibit xylem sap flow and/or photosynthetic product flow in plants; or 3) one or more components that inhibit water flow in irrigation systems. In situ chemistry of existing molecular structures, especially chemical bonds, is facilitated. These in situ chemical reactions 1) dissolve, disperse, or otherwise disrupt one or more components of the pathogen's defense structure and disrupt one or more essential physiological processes; 2) dissolve, disperse, or otherwise inhibit one or more components that inhibit xylem sap flow at the xylem and/or phloem photosynthate flow; or 3) dissolve one or more components that inhibit water flow within the pipeline network of the perfusion system; , disperse, or otherwise destroy, improving water distribution that maintains and/or enhances plant health and vitality.

Without wishing to be bound by any theory, application of the botanical drug composition in an aqueous environment results in the spontaneous formation of highly reactive, uniquely structured ultrafine microbubbles. The "functionalized" microbubbles consist of an outer "highly reactive" shell and an air-laden inner core composed of one or more nonionic surfactants and components derived from the treated fermented microbial supernatant. including. A "highly reactive" shell results in greatly enhanced mass transfer of oxygen in an aqueous environment and enhanced biocatalysis of the molecular structure of the compound, both of which combine to provide synergistic functionality. In terms of oxygen mass transfer, this functionality increases the oxygen transfer rate, raising dissolved oxygen levels in aqueous environments well beyond the solubility limits predicted by Henry's law, and mechanical aeration systems. reach levels that are not easily achieved. Components from the treated fermented microbial supernatant appear to interfere with the ability of nonionic surfactants to produce well-organized micellar shells. As a result, the molecular packing of these fermentation components and surfactants that "functionalized" the shell to increase gas permeability relaxes, thereby creating more favorable conditions for gas mass movement. This oxygen transfer function therefore increases the availability of oxygen in an aqueous environment. In terms of promoting biocatalysis, this functionality provides a reaction platform that increases the local concentration of reactants, thereby lowering the energy transitions required for catalysis to occur and allowing electron donation. , promote chemical reactions at electron-deficient sites. This biocatalytic function thus mediates the cleavage of chemical bonds, including glycosidic and ester bonds present in the compound. Thus, the 'functionalized' shell of microbubbles has catalytic activity like conventional enzymatic systems, but does not require any enzymes. Thus, application of the botanical pharmaceutical compositions disclosed herein results in “functionalized” microbubbles that facilitate oxygen diffusion, thereby increasing dissolved oxygen levels and intermolecular interactions. is promoted and catalytic decomposition of the compound occurs.

"functionalized" microbubbles, upon contact with a pathogen defense structure, chemically interact with one or more components of that defense structure such that electron donation or reaction occurs at electron deficient sites; Mediates cleavage of chemical bonds, including glycosidic and ester bonds, present in one or more of its components. Similarly, when "functionalized" microbubbles come into contact with plant xylem sap and/or one or more components that inhibit photosynthetic product flow, electron donation or reaction occurs at electron-deficient sites. chemically interacts with one or more moieties to mediate the cleavage of chemical bonds, including glycosidic and ester bonds, present in the one or more moieties. Similarly, when the "functionalized" microbubbles come into contact with one or more components that impede water flow in an irrigation system, one or more components may be added such that electron donation or reaction occurs at electron deficient sites. and mediate the cleavage of chemical bonds, including glycosidic and ester bonds, present in one or more of its components. The above interaction appears to be a form of hydrolysis using beta-oxidation that, in addition to relying on a 'highly reactive' shell, also utilizes the oxygen present in the core of the microbubbles. Thus, the properties found in "functionalized" microbubbles act synergistically with the oxygen transfer capacity of the nucleus to: 2) one or more components that inhibit plant xylem sap and/or photosynthetic product flow; and/or 3) glycosidic bonds and esters present in one or more components that inhibit water flow in irrigation systems. Facilitates in situ dissolution of chemical bonds, including bonds.

Furthermore, when the 'functionalized' microbubbles come into contact with the root hairs, they increase the permeability of the root hair membrane to water transport, providing a better microbial environment for the commensal organisms to enhance root hair function, thereby enhancing root hair function. It increases absorption, increases nitrogen fixation, increases gas exchange, increases capillary action and hydrostatic pressure in vascular tissue. Such interactions lead to improved uptake by root hairs, improved xylem sap flow through the xylem, and improved photosynthetic product flow at the phloem, contributing to the maintenance and/or enhancement of plant health and vitality. Improved transport of raw materials, growth components and energy used is provided.

Application of the botanical pharmaceutical compositions disclosed herein results in sufficient destruction of one or more components present in the defense structures of plant disease pathogens, followed by one or more essential physiological processes. can be by any method that exposes one or more components of the defensive structure to the botanical pharmaceutical composition of the present disclosure such that the cells collapse and death occurs. For example, exposure can be by direct application to the pathogen or indirect application to the locus where the pathogen is exposed to the botanical drug composition.

Similarly, application of the botanical pharmaceutical compositions disclosed herein may result in increased absorption of water, minerals and other nutrients from the soil, increased capillary action and/or hydrostatic pressure in the xylem and/or Root hairs are exposed to the botanical pharmaceutical compositions of the present disclosure to result in increased synthesis of compounds and energy followed by root hair uptake, xylem sap flow through the xylem and photosynthetic product flow at the phloem. by any method. For example, exposure can be by direct application to one or more plants or indirect application to a locus where one or more plants are exposed to the botanical pharmaceutical composition.

Similarly, application of the botanical pharmaceutical compositions disclosed herein will result in sufficient destruction of one or more components of the defensive structures and then the application of water and nutrients to maintain and/or enhance plant health and vitality. exposing one or more components that inhibit xylem sap flow in the xylem and/or phloem photosynthetic product flow to the botanical pharmaceutical composition of the present disclosure, such that transport of method. For example, exposure can be by direct application to one or more plants or indirect application to a locus where one or more plants are exposed to the botanical pharmaceutical composition.

Further, application of the botanical pharmaceutical compositions disclosed herein is sufficient to destroy one or more components of the defensive structures and then distribute the irrigation system to maintain and/or enhance plant health and vitality. can be by any method that exposes one or more components that inhibit water flow within the pipeline network of the perfusion system to the botanical pharmaceutical compositions of the present disclosure such that the water flow is improved. For example, exposure may be by direct application to one or more pipeline networks of an irrigation system or indirect application where one or more pipeline networks of an irrigation system are exposed to the botanical composition. can be

The methods and uses disclosed herein may employ the botanical compositions disclosed herein in undiluted form. Alternatively, it may be desirable to dilute the botanical compositions disclosed herein, and those skilled in the art will appreciate that dilutions of such compositions may be used. The botanical pharmaceutical compositions disclosed herein are typically carried out with water, but other suitable dilutions may be used as long as they are compatible with the formation of the microbubbles disclosed herein. agent may be used. In aspects of this embodiment, the botanical composition is for example 1:10, 1:25, 1:50, 1:75, 1:100, 1:200, 1:300, 1:400, 1 : 500, 1:600, 1:700, 1:800, 1:900, 1:1000, 1:2000, 1:3000, 1:4000, 1:5000, 1:6000, 1:7000, 1:8000 , 1:9000, 1:10000, 1:20000, 1:30000, 1:40000, 1:50000, 1:60000, 1:70000, 1:80000, 1:90000 or 1:100000. In other aspects of this embodiment, the botanical composition is for example at least 1:10, at least 1:25, at least 1:50, at least 1:75, at least 1:100, at least 1:200, at least 1 :300, at least 1:400, at least 1:500, at least 1:600, at least 1:700, at least 1:800, at least 1:900, at least 1:1000, at least 1:2000, at least 1:3000, at least 1 :4000, at least 1:5000, at least 1:6000, at least 1:7000, at least 1:8000, at least 1:9000, at least 1:10000, at least 1:20000, at least 1:30000, at least 1:40000, at least 1 :50000, at least 1:60000, at least 1:70000, at least 1:80000, at least 1:90000 or at least 1:100000. In yet another aspect of this embodiment, the botanical composition is for example up to 1:10, up to 1:25, up to 1:50, up to 1:75, up to 1:100, up to 1:200, up to 1:300, max 1:400, max 1:500, max 1:600, max 1:700, max 1:800, max 1:900, max 1:1000, max 1:2000, max 1:3000, max 1:4000, max 1:5000, max 1:6000, max 1:7000, max 1:8000, max 1:9000, max 1:10000, max 1:20000, max 1:30000, max 1:40000, max Dilute to a ratio of 1:50000, up to 1:60000, up to 1:70000, up to 1:80000, up to 1:90000 or up to 1:100000.

In yet another aspect of this embodiment, the botanical composition is, for example, about 1:1 to about 1:10, about 1:1 to about 1:25, about 1:1 to about 1:50, about 1:1 to about 1:75, about 1:1 to about 1:100, about 1:2 to about 1:10, about 1:2 to about 1:25, about 1:2 to about 1:50, about 1:2 to about 1:75, about 1:2 to about 1:100, about 1:10 to about 1:25, about 1:10 to about 1:50, about 1:10 to about 1:75, about 1:10 to about 1:100, about 1:10 to about 1:125, about 1:10 to about 1:150, about 1:10 to about 1:175, about 1:10 to about 1:200, about 1:10 to about 1:225, about 1:10 to about 1:250, about 1:50 to about 1:100, about 1:50 to about 1:200, about 1:50 to about 1:300, about 1:50 to about 1:400, about 1:50 to about 1:500, about 1:50 to about 1:600, about 1:50 to about 1:700, about 1:50 to about 1:800, about 1:50 to about 1:900, about 1:50 to about 1:1000, about 1:100 to about 1:200, about 1:100 to about 1:300, about 1:100 to about 1:400, about 1:100 to about 1:500, about 1:100 to about 1:600, about 1:100 to about 1:700, about 1:100 to about 1:800, about 1:100 to about 1:900, about 1:100 to about 1:1000, about 1:500 to about 1:1000, about 1:500 to about 1:2000, about 1:500 to about 1:3000, about 1:500 to about 1:4000, about 1:500 to about 1:5000, about 1:500 to about 1:6000, about 1:500 to about 1:7000, about 1:500 to about 1:8000, about 1:500 to about 1:9000, about 1:500 to about 1:10000, about 1:1000 to about 1:2000, about 1:1000 to about 1:3000, about 1:1000 to about 1:4000, about 1:1000 to about 1:5000, about 1:1000 to about 1:6000, about 1:1000 to about 1:7000, about 1:1000 to about 1:8000, about 1:1000 to about 1:9000, about 1:1000 to about 1:10000, about About About 1:10 000 to about 1:20000, about 1:10000 to about 1:30000, about 1:10000 to about 1:40000, about 1:10000 to about 1:50000, about 1:10000 to about 1:60000, about 1: Dilute to a ratio of 10000 to about 1:70000, about 1:10000 to about 1:80000, about 1:10000 to about 1:90000, about 1:10000 to about 1:100000.

Application of the botanical pharmaceutical compositions disclosed herein is to be carried out in an effective amount. Effective amounts of the disclosed botanical compositions are: 1) an amount sufficient to cause a detrimental effect on the population of pathogens of the plant disease to be controlled; 3) increasing the absorption of water, minerals and other nutrients from the soil, causing capillary action in the xylem and/or 4) an amount sufficient to cause an increase in hydrostatic pressure and/or increase synthesis of compounds and energy; and/or 5) an amount sufficient to cause sufficient removal of one or more components interfering with one or more pipeline networks of the irrigation system. The actual effective amount of a disclosed botanical drug composition is determined by routine screening methods used to evaluate the control activity and effectiveness of the botanical drug compositions disclosed herein. Such screening methods are well known to those of skill in the art. The botanical pharmaceutical compositions disclosed herein can be used in lower amounts and concentrations for higher activity levels, and in the amounts required for lower activity levels, to achieve the same control effect. Or it is expected that the concentration could be greater.

Application of the botanical pharmaceutical compositions disclosed herein is to be carried out in an effective amount. An effective amount of a disclosed botanical pharmaceutical composition can be an amount sufficient to produce the desired effect. The actual effective amount of a disclosed botanical drug composition is determined by routine screening methods used to evaluate the control activity and effectiveness of the botanical drug compositions disclosed herein. Such screening methods are well known to those of skill in the art. The botanical pharmaceutical compositions disclosed herein can be used in lower amounts and concentrations for higher activity levels, and in the amounts required for lower activity levels, to achieve the same control effect. Or it is expected that the concentration could be greater.

An effective amount of a disclosed botanical pharmaceutical composition can be an amount sufficient to cause a detrimental effect on the pathogen to be controlled. In aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition is, for example, about 10%, about 15%, about 20%, about 25%, about 30% of the pathogens of the plant-infecting population. , about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about It is sufficient to cause adverse effects in 95%. In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 15%, at least 20%, at least 25%, at least 30% %, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% or The amount is sufficient to cause adverse effects in at least 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition is a population pathogen that infects a plant, e.g. 30%, max 35%, max 40%, max 45%, max 50%, max 55%, max 60%, max 65%, max 70%, max 75%, max 80%, max 85%, max 90% or sufficient to cause adverse effects in up to 95%. In yet another aspect of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70% , about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about sufficient to cause adverse effects in 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% amount.

In aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition is, for example, about 10%, about 15%, about 20%, about 25%, about 30% of the pathogens of the plant-infecting population. , about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about Enough to kill 95%. In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 15%, at least 20%, at least 25%, at least 30% %, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% or Sufficient to kill at least 95%. In yet another aspect of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, up to 10%, up to 15%, up to 20%, up to 25%, up to 30%, max 35%, max 40%, max 45%, max 50%, max 55%, max 60%, max 65%, max 70%, max 75%, max 80%, max 85%, max 90% Or enough to kill up to 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition is, for example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70% , about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about an amount sufficient to kill 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% is.

An effective amount of the disclosed botanical pharmaceutical compositions can be an amount sufficient to reduce the size of the pathogen population to be controlled. In aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces the population size of the pathogen to be controlled by, for example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90 % or about 95% reduction. In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition reduces the population size of the pathogen to be controlled by, for example, at least 10%, at least 15%, at least 20%, at least 25%. , at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least Sufficient amount for 90% or at least 95% reduction. In yet another aspect of this embodiment, an effective amount of the disclosed botanical composition reduces the size of the pathogen population to be controlled, for example, up to 10%, up to 15%, up to 20%, up to 25%. %, up to 30%, up to 35%, up to 40%, up to 45%, up to 50%, up to 55%, up to 60%, up to 65%, up to 70%, up to 75%, up to 80%, up to 85%, Sufficient amount for up to 90% or up to 95% reduction. In yet another aspect of this embodiment, an effective amount of the disclosed botanical compositions controls the pathogen to be controlled, e.g., about 10% to about 20%, about 10% to about 30%, to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80% , about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80% , about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95%.

An effective amount of a disclosed botanical pharmaceutical composition can be an amount sufficient to deter the invasion or infestation of one or more loci by the pathogen population to be controlled. In aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition is, for example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35% of the pathogen population. , about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about 95% is 1 Sufficient to deter infestation or infestation in one or more locations. In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 15%, at least 20%, at least 35%, at least 35% %, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% or at least 95% Sufficient amount to deter infestation or infestation in one or more locations. In yet another aspect of this embodiment, the effective amount of the disclosed botanical compositions is, for example, up to 10%, up to 15%, up to 20%, up to 25%, up to 30%, up to 35%, up to 40%, up to 45%, up to 50%, up to 55%, up to 60%, up to 65%, up to 70%, up to 75%, up to 80%, up to 85%, up to 90% or up to 95% is sufficient to prevent from invading or infesting one or more locations. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition is, for example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95% , about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90% , about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% infest or infest one or more locations is sufficient to prevent

An effective amount of the disclosed botanical pharmaceutical compositions can be an amount sufficient to improve the absorption of water, minerals and other nutrients from the soil by root hairs. In aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces the absorption of water, minerals and other nutrients from the soil by root hairs by, for example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85 %, about 90% or about 95% improvement. In other aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces the absorption of water, minerals and other nutrients from the soil by root hairs by, for example, at least 10%, at least 15%, at least 20%. , at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least An amount sufficient to provide an 85%, at least 90% or at least 95% improvement. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces the absorption of water, minerals and other nutrients from the soil by root hairs by, for example, up to 10%, up to 15%, up to 20%, %, max 25%, max 30%, max 35%, max 40%, max 45%, max 50%, max 55%, max 60%, max 65%, max 70%, max 75%, max 80%, An amount sufficient to improve up to 85%, up to 90% or up to 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces the absorption of water, minerals and other nutrients from the soil by root hairs by, for example, about 10% to about 20%, about 10%. % to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70% %, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40 % to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95 %, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% improvement enough to

An effective amount of the disclosed botanical pharmaceutical composition can be an amount sufficient to improve xylem sap flow through the xylem. In aspects of this embodiment, an effective amount of the disclosed botanical compositions reduces xylem sap flow through the xylem by, for example, about 10%, about 15%, about 20%, about 25%, about 30%. %, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or A sufficient amount for about a 95% improvement. In other aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces xylem sap flow through the xylem, e.g., at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% Or an amount sufficient to provide at least a 95% improvement. In yet another aspect of this embodiment, an effective amount of the disclosed botanical composition reduces the xylem sap flow through the xylem, for example, up to 10%, up to 15%, up to 20%, up to 25%, 30% max, 35% max, 40% max, 45% max, 50% max, 55% max, 60% max, 65% max, 70% max, 75% max, 80% max, 85% max, 90% max % or up to 95% improvement. In yet another aspect of this embodiment, an effective amount of the disclosed botanical composition reduces xylem sap flow through the xylem, e.g., from about 10% to about 20%, from about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10 % to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70% %, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50 % to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to an amount sufficient to improve about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% is.

An effective amount of the disclosed botanical pharmaceutical compositions can be an amount sufficient to improve photosynthetic product flux in the phloem. In aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical compositions reduces phloem photosynthetic product flux by, for example, about 10%, about 15%, about 20%, about 25%, about 30%. , about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about A sufficient amount for a 95% improvement. In other aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces phloem photosynthetic product flux by, for example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30% %, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% or An amount sufficient to provide at least a 95% improvement. In yet another aspect of this embodiment, an effective amount of the disclosed botanical compositions reduces phloem photosynthetic product flux, e.g., up to 10%, up to 15%, up to 20%, up to 25%, 30%, max 35%, max 40%, max 45%, max 50%, max 55%, max 60%, max 65%, max 70%, max 75%, max 80%, max 85%, max 90% Or an amount sufficient for up to 95% improvement. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces phloem photosynthetic product flux by, for example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70% , about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about in an amount sufficient to improve 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% be.

An effective amount of the disclosed botanical pharmaceutical compositions can be an amount sufficient to increase the absorption of water, minerals and other nutrients from the soil. In aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces absorption of water, minerals and other nutrients from soil by, for example, about 10%, about 15%, about 20%, about 25%. %, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, An amount sufficient to provide an increase of about 90% or about 95%. In other aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces the absorption of water, minerals and other nutrients from the soil by, for example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85% , an amount sufficient to increase at least 90% or at least 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical composition reduces absorption of water, minerals and other nutrients from soil by, for example, up to 10%, up to 15%, up to 20%, 25% max, 30% max, 35% max, 40% max, 45% max, 50% max, 55% max, 60% max, 65% max, 70% max, 75% max, 80% max, 85% max %, up to 90% or up to 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical drug composition reduces the absorption of water, minerals and other nutrients from the soil by, for example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90% %, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30 % to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80% %, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% is sufficient for

An effective amount of the disclosed botanical pharmaceutical compositions can be an amount sufficient to increase capillary action and/or hydrostatic pressure in xylem. In aspects of this embodiment, an effective amount of the disclosed botanical composition reduces capillary action and/or hydrostatic pressure in xylem by, for example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90 % or about 95%. In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition reduces capillary action and/or hydrostatic pressure in the xylem, for example, at least 10%, at least 15%, at least 20%, at least 25% %, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, An amount sufficient to provide an increase of at least 90% or at least 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical drug composition reduces capillary action and/or hydrostatic pressure in xylem, e.g., up to 10%, up to 15%, up to 20%, up to 25%, 30% max, 35% max, 40% max, 45% max, 50% max, 55% max, 60% max, 65% max, 70% max, 75% max, 80% max, 85% max , an amount sufficient to increase up to 90% or up to 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical composition reduces capillary action and/or hydrostatic pressure in the xylem, for example, from about 10% to about 20%, from about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90% , about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80% , about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about to about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% Enough quantity.

An effective amount of a disclosed botanical pharmaceutical composition can be an amount sufficient to improve transport of the material through the plant. In aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces transport of the material through the plant by, for example, about 10%, about 15%, about 20%, about 25%, about 30%. , about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about A sufficient amount for a 95% improvement. In other aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces transport of the material through the plant by, for example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, %, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% or A sufficient amount for at least a 95% improvement. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces transport of the material through the plant by, for example, up to 10%, up to 15%, up to 20%, up to 25%, up to 30%, max 35%, max 40%, max 45%, max 50%, max 55%, max 60%, max 65%, max 70%, max 75%, max 80%, max 85%, max 90% Or an amount sufficient for up to 95% improvement. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces transport of the material through the plant by, for example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70% , about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about in an amount sufficient to improve 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% be.

An effective amount of a disclosed botanical pharmaceutical composition can be an amount sufficient to increase synthesis of compounds and energy within a plant. In aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces compound and energy synthesis in the plant by, for example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% Or an amount sufficient to increase about 95%. In other aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces synthesis of the compound and energy within the plant by, for example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% % or at least 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces synthesis of the compound and energy in the plant by, for example, up to 10%, up to 15%, up to 20%, up to 25%. , up to 30%, up to 35%, up to 40%, up to 45%, up to 50%, up to 55%, up to 60%, up to 65%, up to 70%, up to 75%, up to 80%, up to 85%, up to Sufficient amount for 90% or up to 95% increase. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces the synthesis of the compound and energy within the plant by, for example, about 10% to about 20%, about 10% to about 30%. , about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70% , about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% sufficient to increase by to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% quantity.

An effective amount of the disclosed botanical pharmaceutical compositions can be an amount sufficient to improve the synthesis of compounds and energy necessary for the maintenance and continuation of plant growth. In aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces the synthesis of compounds and energy necessary for the maintenance and continuation of plant growth, e.g., about 10%, about 15%, about 20%. , about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about An amount sufficient to provide an 85%, about 90% or about 95% improvement. In other aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces the synthesis of compounds and energy necessary for the maintenance and continuation of plant growth, e.g., at least 10%, at least 15%, at least 20% %, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, An amount sufficient to provide an improvement of at least 85%, at least 90%, or at least 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces the synthesis of compounds and energy necessary for the maintenance and continuation of plant growth, e.g., up to 10%, up to 15%, up to 20%, max 25%, max 30%, max 35%, max 40%, max 45%, max 50%, max 55%, max 60%, max 65%, max 70%, max 75%, max 80% , an amount sufficient to improve up to 85%, up to 90% or up to 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces the synthesis of compounds and energy necessary for the maintenance and continuation of plant growth, e.g., about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60% , about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95% Enough amount to improve.

An effective amount of the disclosed botanical pharmaceutical compositions is sufficient to dissolve, disperse, or otherwise remove one or more components that inhibit xylem sap flow in the xylem. can be an amount. In aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition contains one or more components that inhibit xylem sap flow in the xylem, e.g., about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80% , about 85%, about 90% or about 95% dissolved, dispersed or otherwise removed. In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition comprises one or more components that inhibit xylem sap flow in the xylem, e.g., at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80 %, at least 85%, at least 90% or at least 95% dissolved, dispersed or otherwise removed. In yet another aspect of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition comprises one or more components that inhibit xylem sap flow in the xylem, e.g., up to 10%, up to 15% , max 20%, max 25%, max 30%, max 35%, max 40%, max 45%, max 50%, max 55%, max 60%, max 65%, max 70%, max 75%, max The amount is sufficient to dissolve, disperse or otherwise remove 80%, up to 85%, up to 90% or up to 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition comprises one or more components that inhibit xylem sap flow in the xylem, for example from about 10% to about 20%. , about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60% , about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about Sufficient to dissolve, disperse or otherwise remove 95%.

An effective amount of a disclosed botanical pharmaceutical composition is an amount sufficient to dissolve, disperse, or otherwise remove one or more components that inhibit photosynthetic product flow in the phloem. can be In aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition comprises one or more components that inhibit phloem photosynthetic product flow, e.g., about 10%, about 15%, about 20% %, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, The amount is sufficient to dissolve, disperse or otherwise remove about 85%, about 90% or about 95%. In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition comprises one or more components that inhibit phloem photosynthetic product flow, e.g., at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80% , at least 85%, at least 90% or at least 95% dissolved, dispersed or otherwise removed. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition comprises one or more components that inhibit photosynthetic product flow in the phloem, e.g., up to 10%, up to 15%, 20% max, 25% max, 30% max, 35% max, 40% max, 45% max, 50% max, 55% max, 60% max, 65% max, 70% max, 75% max, 80% max %, up to 85%, up to 90% or up to 95% dissolved, dispersed or otherwise removed. In yet another aspect of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition comprises one or more components that inhibit phloem photosynthetic product flow, e.g., about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10 % to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60% %, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50 % to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% % dissolved, dispersed or otherwise removed.

An effective amount of the disclosed botanical pharmaceutical compositions is sufficient to dissolve, disperse, or otherwise remove one or more components that inhibit water flow within the pipeline network of the perfusion system. can be a large amount. In aspects of this embodiment, an effective amount of the disclosed botanical composition comprises one or more components that inhibit water flow within the pipeline network of the perfusion system, e.g., about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80 %, about 85%, about 90% or about 95% dissolved, dispersed or otherwise removed. In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition comprises one or more components that inhibit water flow within the pipeline network of the perfusion system, e.g., at least 10%, at least 15% , at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least The amount is sufficient to dissolve, disperse or otherwise remove 80%, at least 85%, at least 90% or at least 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical drug composition contains one or more components that inhibit water flow within the pipeline network of the perfusion system, e.g. %, max 20%, max 25%, max 30%, max 35%, max 40%, max 45%, max 50%, max 55%, max 60%, max 65%, max 70%, max 75%, The amount is sufficient to dissolve, disperse or otherwise remove up to 80%, up to 85%, up to 90% or up to 95%. In yet another aspect of this embodiment, an effective amount of the disclosed botanical drug composition contains one or more components that inhibit water flow within the pipeline network of the perfusion system, for example from about 10% to about 20%. %, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20 % to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60% %, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60 % to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to An amount sufficient to dissolve, disperse or otherwise remove about 95%.

An effective amount of the disclosed botanical pharmaceutical compositions can be an amount sufficient to improve water transport throughout a pipeline network. In aspects of this embodiment, an effective amount of the disclosed botanical composition reduces water transport throughout the pipeline network by, for example, about 10%, about 15%, about 20%, about 25%, about 30%. , about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about A sufficient amount for a 95% improvement. In other aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition reduces water transport throughout the pipeline network by, for example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, %, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% or A sufficient amount for at least a 95% improvement. In yet another aspect of this embodiment, an effective amount of the disclosed botanical composition reduces water transport throughout the pipeline network by, for example, up to 10%, up to 15%, up to 20%, up to 25%, up to 30%, max 35%, max 40%, max 45%, max 50%, max 55%, max 60%, max 65%, max 70%, max 75%, max 80%, max 85%, max 90% Or an amount sufficient for up to 95% improvement. In yet another aspect of this embodiment, an effective amount of the disclosed botanical composition reduces water transport throughout the pipeline network by, for example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70% , about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about in an amount sufficient to improve 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% be.

An effective amount of the disclosed botanical pharmaceutical compositions can be an amount sufficient to maintain and/or enhance plant health and vitality. In aspects of this embodiment, an effective amount of the disclosed botanical compositions maintains plant health and vigor and/or maintains plant health and vigor, for example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% Or an amount sufficient for about a 95% increase. In other aspects of this embodiment, an effective amount of the disclosed botanical pharmaceutical composition maintains plant health and vigor and/or at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% % or at least a 95% increase. In yet another aspect of this embodiment, an effective amount of the disclosed botanical compositions maintains plant health and vigor and/or maintains plant health and vigor, e.g. , up to 30%, up to 35%, up to 40%, up to 45%, up to 50%, up to 55%, up to 60%, up to 65%, up to 70%, up to 75%, up to 80%, up to 85%, up to Sufficient for 90% or up to 95% enhancement. In yet another aspect of this embodiment, an effective amount of the disclosed botanical compositions maintains plant health and vigor and/or , about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70% , about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% sufficient to increase to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95%, or about 90% to about 95% quantity.

An effective amount of a disclosed botanical composition can be a dilution ratio of the botanical composition disclosed herein. In aspects of this embodiment, effective amounts of the disclosed botanical pharmaceutical compositions are, for example, about 1:50, about 1:75, about 1:100, about 1:125, about 1:150, about 1:150, : 175, about 1:200, about 1:225, about 1:250, about 1:275, about 1:300, about 1:325, about 1:350, about 1:375, about 1:400, about 1 is a ratio of botanical composition to diluent of . In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, at least 1:50, at least 1:75, at least 1:100, at least 1:125, at least 1:150, at least 1:175, at least 1:200, at least 1:225, at least 1:250, at least 1:275, at least 1:300, at least 1:325, at least 1:350, at least 1:375, at least 1:400, at least at a ratio of botanical composition to diluent of 1:425, at least 1:450, at least 1:475, at least 1:500, at least 1:525, at least 1:550, at least 1:575 or at least 1:600 be. In yet another aspect of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, up to 1:50, up to 1:75, up to 1:100, up to 1:125, up to 1:150, Max 1:175, Max 1:200, Max 1:225, Max 1:250, Max 1:275, Max 1:300, Max 1:325, Max 1:350, Max 1:375, Max 1:400, ratio of botanical composition to diluent up to 1:425, up to 1:450, up to 1:475, up to 1:500, up to 1:525, up to 1:550, up to 1:575 or up to 1:600 is. In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical compositions is, for example, from about 1:50 to about 1:100, from about 1:50 to about 1:200, from about 1:50 to about 1:300, about 1:50 to about 1:400, about 1:50 to about 1:500, about 1:50 to about 1:600, about 1:100 to about 1:200, about 1:100 to about 1:300, about 1:100 to about 1:400, about 1:100 to about 1:500, about 1:100 to about 1:600, about 1:200 to about 1:300, about 1:200 to about 1:400, about 1:200 to about 1:500, about 1:200 to about 1:600, about 1:300 to about 1:400, about 1:300 to about 1:500, about 1:300 to about The ratio of botanical composition to diluent is 1:600, from about 1:400 to about 1:500, from about 1:400 to about 1:600, or from about 1:500 to about 1:600. The ratio of the botanical composition to the diluent described above is generally suitable for the methods and uses of controlling pathogens of plant diseases of the disclosure, methods and uses of increasing plant growth and/or crop production of the disclosure and plant diseases. is effective for use in controlling pathogens of the present disclosure, as well as in methods and uses for maintaining or improving the efficiency of irrigation systems of the present disclosure.

In aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, about 1:500, about 1:750, about 1:1000, about 1:1250, about 1:1500, about 1 : 1750, about 1:2000, about 1:2250, about 1:2500, about 1:2750, about 1:3000, about 1:3250, about 1:3500, about 1:3750, about 1:4000, about 1 : 4250, about 1:4500, about 1:4750, about 1:5000, about 1:5250, about 1:5500, about 1:5750, about 1:6000, about 1:7000, about 1:8000, about 1 :9000 or about 1:10000 botanical composition to diluent ratio. In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, at least 1:500, at least 1:750, at least 1:1000, at least 1:1250, at least 1:1500, at least 1:1750, at least 1:2000, at least 1:2250, at least 1:2500, at least 1:2750, at least 1:3000, at least 1:3250, at least 1:3500, at least 1:3750, at least 1:4000, at least 1:4250, at least 1:4500, at least 1:4750, at least 1:5000, at least 1:5250, at least 1:5500, at least 1:5750, at least 1:6000, at least 1:7000, at least 1:8000, at least A ratio of botanical composition to diluent of 1:9000 or at least 1:10000. In yet another aspect of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, up to 1:500, up to 1:750, up to 1:1000, up to 1:1250, up to 1:1500, Max 1:1750, Max 1:2000, Max 1:2250, Max 1:2500, Max 1:2750, Max 1:3000, Max 1:3250, Max 1:3500, Max 1:3750, Max 1:4000, Max 1:4250, Max 1:4500, Max 1:4750, Max 1:5000, Max 1:5250, Max 1:5500, Max 1:5750, Max 1:6000, Max 1:7000, Max 1:8000, A ratio of botanical composition to diluent up to 1:9000 or up to 1:10000. In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, from about 1:500 to about 1:1000, from about 1:500 to about 1:2000, from about 1:500 to about 1:3000, about 1:500 to about 1:4000, about 1:500 to about 1:5000, about 1:500 to about 1:6000, about 1:500 to about 1:7000, about 1:500 to about 1:8000, about 1:500 to about 1:9000, about 1:500 to about 1:10000, about 1:1000 to about 1:2000, about 1:1000 to about 1:3000, about 1:1000 to about 1:4000, about 1:1000 to about 1:5000, about 1:1000 to about 1:6000, about 1:1000 to about 1:7000, about 1:1000 to about 1:8000, about 1:1000 to about 1:9000, about 1:1000 to about 1:10000, about 1:2000 to about 1:3000, about 1:2000 to about 1:4000, about 1:2000 to about 1:5000, about 1:2000 to about 1:6000, about 1:2000 to about 1:7000, about 1:2000 to about 1:8000, about 1:2000 to about 1:9000, about 1:2000 to about 1:10000, about 1:3000 to about 1:4000, about 1:3000 to about 1:5000, about 1:3000 to about 1:6000, about 1:3000 to about 1:7000, about 1:3000 to about 1:8000, about 1:3000 to about 1:9000, about 1:3000 to about 1:10000, about 1:4000 to about 1:5000, about 1:4000 to about 1:6000, about 1:4000 to about 1:7000, about 1:4000 to about 1:8000, about 1:4000 to about 1:9000, about 1:4000 to about 1:10000, about 1:5000 to about 1:6000, about 1:5000 to about 1:7000, about 1:5000 to about 1:8000, about 1:5000 to about 1:9000, about 1:5000 to about 1:10000, about 1:6000 to about 1:7000, about 1:6000 to about 1:8000, about 1:6000 to about 1:9000, about 1:6000 to about 1:10000, about 1:7000 to about 1:8000, about 1:7000 to about 1:9000, about 1:7000 to about 1:10000, about 1:8000 to about The ratio of botanical composition to diluent is 1:9000, from about 1:8000 to about 1:10000 or from about 1:9000 to about 1:10000. The ratio of the botanical composition to the diluent described above is generally suitable for the methods and uses of controlling pathogens of plant diseases of the disclosure, methods and uses of increasing plant growth and/or crop production of the disclosure and plant diseases. is effective for use in controlling pathogens of the present disclosure, as well as in methods and uses for maintaining or improving the efficiency of irrigation systems of the present disclosure.

In aspects of this embodiment, effective amounts of the disclosed botanical pharmaceutical compositions are, for example, about 0.0001%, about 0.0002%, about 0.0003%, about 0.0004%, about 0.0004%, 0005%, about 0.0006%, about 0.0007%, about 0.0008%, about 0.0009%, about 0.001%, about 0.002%, about 0.003%, about 0.004% , about 0.005%, about 0.006%, about 0.007%, about 0.008%, about 0.009%, about 0.01%, about 0.02%, about 0.03%, about 0.04%, about 0.05%, about 0.06%, about 0.07%, about 0.08%, about 0.09%, about 0.1%, about 0.2%, about 0.2% 3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1%, about 2%, about 3% , about 4%, about 5%, about 6%, about 7%, about 8%, about 9% or about 10% final concentration. In other aspects of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, at least 0.0001%, at least 0.0002%, at least 0.0003%, at least 0.0004%, at least 0.0004%, at least 0.0004%, at least 0.0004%, .0005%, at least 0.0006%, at least 0.0007%, at least 0.0008%, at least 0.0009%, at least 0.001%, at least 0.002%, at least 0.003%, at least 0.004 %, at least 0.005%, at least 0.006%, at least 0.007%, at least 0.008%, at least 0.009%, at least 0.01%, at least 0.02%, at least 0.03%, at least 0.04%, at least 0.05%, at least 0.06%, at least 0.07%, at least 0.08%, at least 0.09%, at least 0.1%, at least 0.2%, at least 0 .3%, at least 0.4%, at least 0.5%, at least 0.6%, at least 0.7%, at least 0.8%, at least 0.9%, at least 1%, at least 2%, at least 3% %, at least 4%, at least 5%, at least 6%, at least 7%, at least 8%, at least 9% or at least 10% final concentration. In yet another aspect of this embodiment, the effective amount of the disclosed botanical composition is, for example, up to 0.0001%, up to 0.0002%, up to 0.0003%, up to 0.0004%, up to 0.0005%, 0.0006% max, 0.0007% max, 0.0008% max, 0.0009% max, 0.001% max, 0.002% max, 0.003% max, 0.003% max 0.005%, 0.006%, 0.007%, 0.008%, 0.009%, 0.01%, 0.02%, 0.03% , max 0.04%, max 0.05%, max 0.06%, max 0.07%, max 0.08%, max 0.09%, max 0.1%, max 0.2%, max 0.3%, max 0.4%, max 0.5%, max 0.6%, max 0.7%, max 0.8%, max 0.9%, max 1%, max 2%, max Final concentrations of 3%, 4% max, 5% max, 6% max, 7% max, 8% max, 9% max or 10% max. In yet another aspect of this embodiment, the effective amount of the disclosed botanical pharmaceutical composition is, for example, about 0.0001% to about 0.0005%, about 0.0001% to about 0.001%, about 0.0001% to about 0.005%; about 0.0001% to about 0.01%; about 0.0001% to about 0.05%; about 0.0001% to about 0.1%; 0001% to about 0.5%, about 0.0001% to about 1%, about 0.0001% to about 5%, about 0.0001% to about 10%, about 0.0005% to about 0.001% , about 0.0005% to about 0.005%, about 0.0005% to about 0.01%, about 0.0005% to about 0.05%, about 0.0005% to about 0.1%, about 0.0005% to about 0.5%, about 0.0005% to about 1%, about 0.0005% to about 5%, about 0.0005% to about 10%, about 0.001% to about 0.5% 005%, about 0.001% to about 0.01%, 0.001% to about 0.05%, about 0.001% to about 0.1%, 0.001% to about 0.5%, 0 .001% to about 1%, 0.001% to about 5%, about 0.001% to about 10%, about 0.005% to about 0.01%, about 0.005% to about 0.05% , about 0.005% to about 0.1%, about 0.005% to about 0.5%, about 0.005% to about 1%, about 0.005% to about 5%, about 0.005% to about 10%, about 0.01% to about 0.05%, about 0.01% to about 0.1%, about 0.01% to about 0.5%, about 0.01% to about 1% , about 0.01% to about 5%, about 0.01% to about 10%, about 0.05% to about 0.1%, about 0.05% to about 0.5%, about 0.05% to about 1%, from about 0.05% to about 5%, from about 0.05% to about 10%, from about 0.1% to about 0.5%, from about 0.1% to about 1%, from about 0.5% 1% to about 5%, about 0.1% to about 10%, about 0.5% to about 1%, about 0.5% to about 5%, about 0.5% to about 10%, about 1% to about 5%, about 1% to about 10% or about 5% to about 10% final concentration.

The effects of the botanical pharmaceutical compositions disclosed herein may include, but are not limited to, adverse effects, mortality, reduction of pathogen populations, reduction of infestation or infestation at one or more sites, or reduction of pathogen growth. Any other measure of damage to a pathogen population, including inhibition, arrest or delay, inhibition, arrest or delay of pathogen reproduction or inhibition, arrest or delay of pathogen development. , any of the items listed above are encompassed by the term "control". In addition, phytotoxicity to plants infested with pathogen populations, tissue damage to host plants infected with pathogen populations, as well as infested plants or plants exposed to the botanical pharmaceutical compositions disclosed herein. Efficacy is monitored by any adverse effects that may be experienced by humans applying the botanical pharmaceutical compositions disclosed in . Accordingly, the amount of a botanical composition disclosed herein for use in a method or use of the disclosure is such that it meets the criteria for an effective amount described above, and is preferably for ornamental and agricultural plants (such as phytotoxicity). ), exhibit minimal or no adverse effects on wildlife and humans that may come into contact with such compositions.

Application of the botanical pharmaceutical compositions disclosed herein may be carried out by any process that efficiently generates the microbubbles disclosed herein and effectively exposes the pathogens to be controlled. can be done. For example, any method that allows the introduction of high concentrations of gas into the botanical composition upon application is suitable as the introduction of such gas naturally forms microbubbles. Suitable application processes include, but are not limited to, spraying, smoking, atomizing, vaporizing, spraying, watering, jetting, watering, and the like. One preferred method of application is manual or mechanical application by irrigation, spraying, smoking, spraying or vaporization. Such applications form a fine mist that is sufficiently aerated in the process to generate the microbubbles disclosed herein. Gas-exposed microbubbles dispersed in a liquid exhibit colloidal properties and are called colloidal gas aphrons (CGA). CGA differs from normal foam in that it has a unique shell layer containing a low concentration of surfactant.

The microbubbles formed by the botanical pharmaceutical compositions disclosed herein are believed to enhance the mass transfer of oxygen in liquids. Without wishing to be bound by any scientific theory, there are several possible reasons for this difference. First, the surfactants formulated in the botanical pharmaceutical compositions disclosed herein include nonionic surfactants and/or biosurfactants, which significantly alter the properties of foam behavior. . Second, the botanical pharmaceutical compositions disclosed herein require much less surfactant concentration for microbubble formation. It has been suggested that the surfactant concentration should be approximately equal to the critical micelle concentration (CMS) of the surfactant system. The botanical pharmaceutical compositions disclosed herein form microbubbles below the estimated CMC of the surfactant used. This suggests that the microbubbles are the result of agglomeration of surfactant molecules with loose molecular packing that is more favorable for gas mass transfer properties. Fewer surfactant molecules on the surface are believed to be more gas permeable than gas-containing, well-organized micelles. Regardless of mechanism, the tendency of the botanical pharmaceutical compositions disclosed herein to come together to form clusters, aggregates or gas-filled bubbles can increase the local concentration of reactants, catalyze reactions, Reducing the energy transitions required to occur, or by some other mechanism yet to be described, provides a platform for the reaction to occur.

In aspects of this embodiment, the microbubbles disclosed herein have an average diameter of, for example, about 5 μm, about 10 μm, about 15 μm, about 20 μm, about 25 μm, about 30 μm, about 40 μm, about 50 μm, about 75 μm, about 100 μm, about 150 μm, about 200 μm, about 250 μm, about 300 μm, about 350 μm, about 400 μm, about 450 μm, about 500 μm, about 550 μm, about 600 μm, about 650 μm, about 700 μm, about 750 μm, about 800 μm, about 850 μm, about 900 μm, about 950 μm or about 1000 μm. In other aspects of this embodiment, the microbubbles disclosed herein have an average diameter, e.g. at least 100 μm, at least 150 μm, at least 200 μm, at least 250 μm, at least 300 μm, at least 350 μm, at least 400 μm, at least 450 μm, at least 500 μm, at least 550 μm, at least 600 μm, at least 650 μm, at least 700 μm, at least 750 μm, at least 800 μm, at least 850 μm, at least 900 μm , at least 950 μm or at least 1000 μm. In other aspects of this embodiment, the microbubbles disclosed herein have an average diameter, e.g. 100 μm max, 150 μm max, 200 μm max, 250 μm max, 300 μm max, 350 μm max, 400 μm max, 450 μm max, 500 μm max, 550 μm max, 600 μm max, 650 μm max, 700 μm max, 750 μm max, 800 μm max, 850 μm max, 900 μm max , up to 950 μm or up to 1000 μm.

In aspects of this embodiment, the microbubbles disclosed herein have an average diameter, e.g. 5 μm to about 30 μm, about 5 μm to about 40 μm, about 5 μm to about 50 μm, about 5 μm to about 75 μm, about 5 μm to about 100 μm, about 10 μm to about 15 μm, about 10 μm to about 20 μm, about 10 μm to about 25 μm, about 10 μm or more about 30 μm, about 10 μm to about 40 μm, about 10 μm to about 50 μm, about 10 μm to about 75 μm, about 10 μm to about 100 μm, about 15 μm to about 20 μm, about 15 μm to about 25 μm, about 15 μm to about 30 μm, about 15 μm to about 40 μm About 20 μm to about 100 μm, about 25 μm to about 30 μm, about 25 μm to about 40 μm, about 25 μm to about 50 μm, about 25 μm to about 75 μm, about 25 μm to about 100 μm, about 30 μm to about 40 μm, about 30 μm to about 50 μm, about 30 μm to about 75 μm, about 30 μm to about 100 μm, about 40 μm to about 50 μm, about 40 μm to about 75 μm, about 40 μm to about 100 μm, about 50 μm to about 75 μm, about 50 μm to about 100 μm, about 50 μm to about 150 μm, about 50 μm to about 200 μm About 50 μm to about 650 μm, about 50 μm to about 700 μm, about 50 μm to about 750 μm, about 50 μm to about 800 μm, about 50 μm to about 850 μm, about 50 μm to about 900 μm, about 50 μm to about 950 μm, about 50 μm to about 1000 μm, about 100 μm to about 150 μm, about 100 μm to about 200 μm, about 100 μm to about 250 μm, about 100 μm to about 300 μm, about 100 μm to about 350 μm, about 100 μm to about 400 μm, about 100 μm to about 450 μm, about 100 μm to about 500 μm, about 100 μm to about 550 μm , about 100 μm to about 600 μm, about 100 μm to about 650 μm, about 100 μm to about 700 μm, about 100 μm to about 750 μm, about 100 μm to about 800 μm, about 100 μm to about 850 μm, about 100 μm to about 900 μm, about 100 μm to about 950 μm, about 100 μm to about 1000 μm, about 150 μm to about 200 μm, about 150 μm to about 250 μm, about 150 μm to about 300 μm, about 150 μm to about 350 μm, about 150 μm to about 400 μm, about 150 μm to about 450 μm, about 150 μm to about 500 μm, about 150 μm to about 550 μm, about 150 μm to about 600 μm, about 150 μm to about 650 μm, about 150 μm to about 700 μm, about 150 μm to about 750 μm, about 150 μm to about 800 μm, about 150 μm to about 850 μm, about 150 μm to about 900 μm, about 150 μm to about 950 μm, about 150 μm to about 1000 μm, about 200 μm to about 250 μm, about 200 μm to about 300 μm, about 200 μm to about 350 μm, about 200 μm to about 400 μm, about 200 μm to about 450 μm, about 200 μm to about 500 μm, about 200 μm to about 550 μm, about 200 μm to about 600 μm, about 200 μm to about 650 μm, about 200 μm to about 700 μm, about 200 μm to about 750 μm, about 200 μm to about 800 μm, about 200 μm to about 850 μm, about 200 μm to about 900 μm, about 200 μm to about 950 μm, about 200 μm to about 1000 μm, about 250 μm to about 300 μm, about 250 μm to about 350 μm, about 250 μm to about 400 μm, about 250 μm to about 450 μm, about 250 μm to about 500 μm, about 250 μm to about 550 μm, about 250 μm to about 600 μm, about 250 μm to about 650 μm, about 250 μm to about 700 μm, about 250 μm to about 750 μm, about 250 μm to about 800 μm, about 250 μm to about 850 μm, about 250 μm to about 900 μm, about 250 μm to about 950 μm, about 250 μm to about 1000 μm, about 300 μm to about 350 μm, about 300 μm to about 400 μm, about 300 μm to about 450 μm, about 300 μm to about 500 μm, about 300 μm to about 550 μm, about 300 μm to about 600 μm, about 300 μm to about 650 μm, about 300 μm to about 700 μm, about 300 μm to about 750 μm, about 300 μm to about 800 μm, about 300 μm to about 850 μm, about 300 μm to about 900 μm, about 300 μm to about 950 μm, about 300 μm to about 1000 μm, about 350 μm to about 400 μm, about 350 μm to about 450 μm, about 350 μm to about 500 μm, about 350 μm to about 550 μm, about 350 μm to about 600 μm, about 350 μm to about 650 μm, about 350 μm to about 700 μm, about 350 μm to about 750 μm, about 350 μm to about 800 μm, about 350 μm to about 850 μm, about 350 μm to about 900 μm, about 350 μm to about 950 μm, about 350 μm to about 1000 μm, about 400 μm about 450 μm, about 400 μm to about 500 μm, about 400 μm to about 550 μm, about 400 μm to about 600 μm, about 400 μm to about 650 μm, about 400 μm to about 700 μm, about 400 μm to about 750 μm, about 400 μm to about 800 μm, about 400 μm to about 850 μm About 450 μm to about 750 μm, about 450 μm to about 800 μm, about 450 μm to about 850 μm, about 450 μm to about 900 μm, about 450 μm to about 950 μm, about 450 μm to about 1000 μm, about 500 μm to about 550 μm, about 500 μm to about 600 μm, about 500 μm about 650 μm, about 500 μm to about 700 μm, about 500 μm to about 750 μm, about 500 μm to about 800 μm, about 500 μm to about 850 μm, about 500 μm to about 900 μm, about 500 μm to about 950 μm, about 500 μm to about 1000 μm, about 550 μm to about 600 μm About 600 μm to about 650 μm, about 600 μm to about 700 μm, about 600 μm to about 750 μm, about 600 μm to about 800 μm, about 600 μm to about 850 μm, about 600 μm to about 900 μm, about 600 μm to about 950 μm, about 600 μm to about 1000 μm, about 650 μm about 700 μm, about 650 μm to about 750 μm, about 650 μm to about 800 μm, about 650 μm to about 850 μm, about 650 μm to about 900 μm, about 650 μm to about 950 μm, about 650 μm to about 1000 μm, about 700 μm to about 750 μm, about 700 μm to about 800 μm , about 700 μm to about 850 μm, about 700 μm to about 900 μm, about 700 μm to about 950 μm, about 700 μm to about 1000 μm About 800 μm to about 1000 μm, about 850 μm to about 900 μm, about 850 μm to about 950 μm, about 850 μm to about 1000 μm, about 900 μm to about 950 μm, about 900 μm to about 1000 μm, or about 950 μm to about 1000 μm.

Aspects of the present specification disclose, in part, plants. Plants include, by way of example, plants, plant populations or parts of plants, as well as specific land areas such as lawns, gardens or farmlands. As used herein, the term "plant" refers to any organism belonging to the kingdom Plantae and forming the phylum Chlorophyta. Non-limiting examples include flowering plants, conifers and other gymnosperms, pteridophytes, lycopods, hornworts, bryophytes, mosses and green algae, but also red algae, brown algae, fungi, archaea, Bacteria and animals are not included in this. Vascular plants include lycophytes, horsetails, ferns, gymnosperms (including conifers) and angiosperms (flowering plants). Scientific names for this group include Tracheophyta and Tracheobionta. As used herein, the term "flower" is synonymous with "bloom" or "blossom" and refers to reproductive structures found in angiosperms. The term "crop plant" as used herein refers to a plant from which a crop is obtained. Non-limiting examples include plants from which fruits, seeds, nuts, grains, oils, wood and fibers are obtained. As used herein, the term "crop" refers to an economically valuable plant product. Non-limiting examples include fruits, seeds, nuts, grains, oils, wood and fibers.

Aspects of the present specification disclose, in part, locations. Thus, the botanical pharmaceutical compositions disclosed herein can be used in, but not limited to, home, lawn and garden, agriculture, organic farming, greenhouse and nursery, storage produce, professional planters, foliage, underwater or underwater applications. , soil mixing, nursery box treatment, intrastem injection and plant treatment applications.

Plant diseases that can be treated by the botanical pharmaceutical compositions, methods and/or uses disclosed herein include, but are not limited to, anthracnose, blight, canker, clubroot, damping off. , gall, flat blast, spot, powdery mildew, mold, mosaic virus, rot, rust, scab, smut and wilt.

Anthracnose or bird's eye disease is caused by a variety of fungi belonging to the genera Colletotrichum, Gloeosporium, Glomerella and Elsinoe, and affects a variety of plants in warm and humid regions. refers to a group of plant diseases that The anthracnose fungus usually infects growing shoots and leaves, producing spores in sporangia known as finely-drilled conidia. Symptoms include small dimpled necrotic patches or lesions with raised borders of various colors on leaves, stems, fruits or flowers and, in some infections, ulceration of twigs and branches. Anthrax causes wilting, withering and necrosis of tissue, but the severity of the infection depends on both the pathogen and the plant species infecting and can range from mere unsightliness to necrosis. Shade trees such as sycamore, ash, oak and maple are particularly susceptible, and the disease is found in several plants, including grasses and annuals. Examples of anthracnose include, but are not limited to, grape anthracnose (Elsinoe ampelina), oyster anthracnose (Gloeosporium kaki), strawberry anthracnose (Glomerella cingulata) 、ウリ炭疽病（コレトトリクム・ラゲナリウム（Ｃｏｌｌｅｔｏｔｒｉｃｈｕｍ ｌａｇｅｎａｒｉｕｍ））、インゲンマメ炭疽病（コレトトリクム・リンデムチアヌム（Ｃｏｌｌｅｔｏｔｒｉｃｈｕｍ ｌｉｎｄｅｍｔｈｉａｎｕｍ））、チャ炭疽病（コレトトリクム・テアエシネンシス（Ｃｏｌｌｅｔｏｔｒｉｃｈｕｍ ｔｈｅａｅｓｉｎｅｎｓｉｓ））およびタバコ炭疽病（コレトトリクム・タバクム（Ｃｏｌｌｅｔｏｔｒｉｃｈｕｍ tabacum)).

Blight is caused by a wide variety of fungi and bacteria and is characterized by symptoms such as rapid and severe bleaching, yellowing, browning, mottling, wilt and subsequent necrosis of plant tissues such as leaves, branches, twigs or flower vases. refers to a group of plant diseases that Head blight, both fungal and bacterial, is most common under cold, damp conditions and usually affects shoots and other young, fast-growing tissues of plants. Most economically important plants including tomatoes, potatoes and apples and many ornamental plant species are susceptible to one or more blight. Blight blight is often named after its causative pathogen. It is named after the fungus Phytophthora parasitica. Examples of the blight disease include, but are not limited to, Alternaria blight (summer blight) (Alternaria solani), Fusarium blight (Fusarium gaminearum), F. avenaceum ( F. avenaceum), F. culmorum, Microdochium nivale), Phytophthora blight (Leaf blight) (Phytophthora infestans), Rhizoctonia seedling blight (Rhizoctonia solani (Rhizoctonia solani); Rhizoctonia leaf blight (Rhizoctonia solani), snow rot brown sclerotia (Typhula sp.), apple monilia (Monilinia mali) , rice bacterial leaf blight (Xanthomonas oryzae), chestnut blight (Cryphonectria parasitica), pome burn (Erwinia amylovora), ring spot disease (Pestalothiopsis spp.), leaf blight (Septoria chrysanthemi-indici), Thanatephorus cucumeris), net blister (Exobasidium reticulatum) (Exobasidium reticulatum)), sheath blight (Rhizoctonia solani), peanut white silk (Sclerotium rolfsii), corn leaf blight (Cochliobolus heterostrophus) , Bipolaris maydis), soybean black spot (Diaporthe phaseolorum var. sojae) and vine blister (Mycosphaerella melonis) is mentioned.

Canker diseases refer to a common and widespread group of plant diseases caused by a wide variety of fungi and bacteria and found primarily in a variety of woody plants. Symptoms include round to irregular, sunken, swollen, flattened, or cracked, discolored, withered, and necrotic areas on stems (cane), twigs, limbs, or trunks. Canker can cause swollen twigs or branches to encircle and wither foliage beyond. Canker disease can structurally weaken plants to the point of breaking in wind and frosty weather. Canker disease also slows normal healing of wounds and introduces fungi and other microorganisms that cause wood rot or wilting. They are most common in plants weakened by cold or drought stress, insect damage, nutritional imbalance, nematodes or root rot. Canker diseases are classified into annual (Fusarium canker), perennial (Nectria canker, Eutipella canker) and generalized canker (Botriosperia canker, Phytophthora canker, Chitospora canker). be able to. Examples of canker diseases include, but are not limited to, apple canker (Valsa ceratosperma), Botryosphaeria canker, Chitospora canker, Eutipera (maple) canker, Nectria canker, Phytophthora blight , fungal ulcer diseases such as Urnula (oak) ulcer disease and bacterial ulcer diseases such as Erwinian ulcer disease, Pseudomonas syringae and Xanthomonas ulcer disease.

Knot disease is caused by the soil fungus Plasmodiophora brassicae and refers to a group of plant diseases affecting members of the cabbage family, causing distorted (clubbed) deformation of roots and often , cracking and rotting. As a result, it becomes difficult for plants to properly absorb water and nutrients. In addition to slowing plant growth, plants may wilt during the warmer days as they recover during cooler nights. Eventually the outer leaves turn yellow or brown. Clubroot reduces yield and can even destroy the entire plant.

Damping off affects seeds and new seedlings and is caused by the genera Fusarium, Phytophthora, Pythium and R. A group of plant diseases caused by several fungi, including species belonging to the genus Rhizoctonia, including R. solani, characterized by symptoms such as decay of stem and root tissues below the soil surface. and In most cases, the infected plants germinate and begin to sprout, but within a few days they become waterlogged, disfigured, collapse at the base and die.

Galle refers to a group of plant diseases caused by fungi, bacteria, viruses and nematodes and certain insects and characterized by symptoms such as abnormal, localized growth or enlargement of plant tissues. Galle infects and kills vines, ornamental flowers, fruit trees, brambles, shade trees and vegetables. In addition, gall occurs as a secondary consequence of infection with plant diseases including cedar-apple rust; clubroot and corn smut. Examples of gall include, but are not limited to, melanoma (Dibotryon morbosum) and crown gall (Agrobacterium tumefaciens).

Plain blast, also called leaf curl, refers to a group of plant diseases that affect many woody and fern plants worldwide, caused by fungi of the genus Taphrina, characterized by curling and curling leaves. and If the weather is cool and humid at the time of germination, the leaves are swollen, twisted, warped, and yellow, red, purple, brown, white, or gray rashes appear on the upper surface of the leaf, and gray pits appear on the lower surface of the leaf. . Such leaves usually die and fall prematurely, weakening the plant. Leaf defoliation from plain blast can greatly reduce fruit production and even kill trees. Plain blast affects peaches, nectarines, plums, almonds, apricots, apricots, birches, cherries, bakutin, California horse chestnut, alder, oak and poplar. Examples of filamentous fungi include, but are not limited to, leaf curl (Taphrina deformans).

Leaf spot refers to a group of plant diseases caused by a large number of fungi and bacteria and characterized by symptoms such as spotting of plant leaves. Infected plants show brown or black, usually uniformly sized, water-soaked spots on the leaves, which may be accompanied by yellow halos. Spots grow and blend under wet conditions. Under dry conditions, the spots have the appearance of small blotches. As the number of spots increases, the entire leaf turns yellow and may wither and fall off. Fungal spot diseases include Alternaria, Asterina, Asterinella, Cercospora, Cercosporella, Cochliobolus, Corynespora , Diplocarpon, Diplotheca, Gloeocercospora, Glomerella, Gnomonia, Phomopsis, Placosphaeria, Pirenophora ( Pyrenophora, Schizothyrium, Sclerotinia, Septoria and Stigmea species. In addition to affecting lettuce, fungal spot can also be found on cruciferous and other vegetables, including cabbage, cauliflower, Chinese cabbage, broccoli, Brussels sprouts, kohlrabi, kale, turnips and rutabaga. Fungal leaf spot also infects strawberries and aspen and poplar. Spot disease also causes several other problems. Fungal spot diseases include Pseudomonas species. Bacterial spot disease particularly affects members of the Prunus genus (drupes including cherries, plums, almonds, apricots and peaches), which are particularly susceptible to bacterial spot disease. Fruit may be speckled or have brown depressions. Bacterial leaf spot also infects tomato and pepper crops and some annual and perennial flowering plants, including roses, geraniums, zinnias, purple roses and pinworts. Examples of wilt spot disease include, but are not limited to, Alternaria spot disease (Alternaria alternata, Alternaria brassicicola, Alternaria japonica), Black spot (Alternaria alternata, Diplocarpon rosae), brown spot (Alternaria longipes), Cochliobolus miyabeanus, Fomopsis vexans ( Phomopsis vexans, Septoria glycines), cercospora spot (Cercospora beticola), dollar spot (Sclerotinia homeocarpa), early spot (Cercospora persona) personata)), soybean spot (Cercospora sojina), gray spot (Cercospora zeae-maydis), black stain (Cercospora arachidicola), spot ( Cercospora kaki, Mycosphaerella nawae, yellow spot (Pyrenophora tritici-repentis), white spot (Cercosporella brassicae, brown spot (Cercosporella brassicae) Corynespora cassiicola) and leaf blight (Gloeocercospora sorghi).

Powdery mildew refers to a group of plant diseases caused by numerous fungi and characterized by symptoms such as growth of a white, gray, bluish or purple powder, usually on the upper or lower leaf surfaces. Small black spots appear and produce spores that are blown away by the wind and infect new plants. When powdery mildew is widespread, leaves turn brown, often wither and die prematurely, and fruits ripen prematurely and are less crunchy and less flavorful. Seedlings may wilt and fall. Hundreds of species of trees, shrubs, vines, flowers, vegetables, fruits, grasses, crops, horticultural plants, shrub fruits and weeds can be affected by powdery mildew. Examples of powdery mildew include, but are not limited to, downy mildew (Basidiophora spp., Bremia lactucae and other Bremia spp., Erysiphe graminis, Plasmopara・ビティコラ（Ｐｌａｓｍｏｐａｒａ ｖｉｔｉｃｏｌａ）、ポドスフェラ属（Ｐｏｄｏｓｐｈａｅｒａ）、ペロノスポラ・デストラクター（Ｐｅｒｏｎｏｓｐｏｒａ ｄｅｓｔｒｕｃｔｏｒ）、ペロノスポラ・パラシチカ（Ｐｅｒｏｎｏｓｐｏｒａ ｐａｒａｓｉｔｉｃａ）、ペロノスポラ・スパルサ（Ｐｅｒｏｎｏｓｐｏｒａ ｓｐａｒｓａ）、ペロノスポラ・タバシナ（Ｐｅｒｏｎｏｓｐｏｒａ ｔａｂａｃｉｎａ）および他のペロノスポラ（ Peronospora spp., Phytophthora spp., Plasmopara spp., Pseudoperonospora cubensis and other Pseudoperonospora spp. and Sclerospora spp. and Sclerospora spp. Powdery mildew (Erysiphe cichoracearum, Erysiphe graminis, Erysiphe pisi and other Erysiphe species, Microsphaera, Filactinia, Phyllactinia)・リューコトリカ（Ｐｏｄｏｓｐｈａｅｒａ ｌｅｕｃｏｔｒｉｃｈａ）および他のポドスフェラ（Ｐｏｄｏｓｐｈａｅｒａ）菌種、スフェロセーカ・フリギネア（Ｓｐｈａｅｒｏｔｈｅｃａ ｆｕｌｉｇｉｎｅａ）、スフェロセーカ・フムリ（Ｓｐｈａｅｒｏｔｈｅｃａ ｈｕｍｕｌｉ）、スフェロセーカ・パンノサ（Ｓｐｈａｅｒｏｔｈｅｃａ ｐａｎｎｏｓａ）および他のスフェロセーカ（Ｓｐｈａｅｒｏｔｈｅｃａ）菌種およびウンキヌラ• Uncinula necator and other Uncinula species).

Mold diseases refer to a group of plant diseases caused by several fungi and characterized by symptoms such as a powdery or woolly appearance on the surface of infected plant parts. Mold diseases affect a variety of plants, including cereals, forage grasses and turfgrass, especially during periods of wet, cool to warm weather. Examples of mold diseases include, but are not limited to, Botrytis cinerea, leaf mold (Cladosporium fulvum), red snow rot (Fusarium nivale) and snow rot (Fusarium nivale). rot (Typhula itoana).

Mosaic virus disease refers to a group of plant diseases caused by plant viruses and characterized by symptoms such as the appearance of several nutrient deficiencies. Yellow streaks or spots on foliage, wrinkled and rounded leaves, stunted growth, and reduced yield. Infected fruits have a patchy appearance, with raised "warty" areas appearing. Mosaic viruses affect a wide variety of plants including roses, beans, tobacco, tomatoes, potatoes and peppers. Examples of mosaic virus species include, but are not limited to, beet mosaic virus, plumpox virus (potyvirus species), tobacco mosaic virus (tobamovirus species), cassava mosaic virus (begomovirus species), cowpea mosaic virus, cucumber mosaic virus, Alfalfa mosaic virus, millet mosaic satellite virus, pumpkin mosaic virus, tulip mosaic virus and zucchini yellow mosaic virus.

Rot, also called rot, refers to a group of plant diseases caused by any of hundreds of types of soil fungi and bacteria and characterized by symptoms of plant decomposition and decay. Decomposition and rot are due to decay of roots, stems, trees, flowers and/or fruits. Decay can be hard and dry, soft and mushy, watery, disfigured, or slimy, and can affect any plant part. Many rots are very active on stored fruit, roots, bulbs or tubers. Some scabs cause leaf rot, but the symptoms tend to be speck and blight. Examples of rot include, but are not limited to, Aphanomyces root rot (Aphanomyces cochlioides), Phomopsis rot (Phomopsis spp.), late blight (Phytophtora cactorum, Phytophthora cactorum). Phytophthora sojae, other Phytophthora species), Sclerotinia (Sclerotinia sclerotiorum), Bitter rot (Colletotrichum acutatum), Black rot (Guignardia bidwellii), brown rot (Monilinia fructicola), root rot (Phytophtora cactorum), fruit rot (Penicillium digitatum, Pigitatum) P. italicum), mushroom rot, scarlet rot (Phytophthora erythroseptica), late rot (Glomerella cingulata), root rot (Aphanomyces spp. Armillaria mellea, Clitcybe tabescens, Fusarium spp., Pythium spp., Phytophthora spp., Thanatephorus cucumeris, stem rot and stem rot wood rot disease.

Caused by more than 5,000 species of fungi, rust is a yellow, orange, red, reddish-brown, brown or black wart that covers the leaves, young shoots and fruits of thousands of economically important plants. It refers to a group of plant diseases characterized by symptoms such as the appearance of ridges. Plant growth and fertility usually decline, and some plants gradually die, leaving only the roots. Rust fungi parasitize one species of plant (homoparasitic rust or monoecious rust) or two different species (heteroparasitic rust) in their life cycle. Alloparasitic rusts include those that affect asparagus, beans, chrysanthemums, coffee, hollyhocks, snapdragons and sugarcane. Xenoparasitic rusts include those that affect cereals, grasses, juniper, fir, poplar, apple, bok choy, hawthorn, rose, currant and gooseberry. Examples of rust include, but are not limited to, asparagus rust, barley rust (Puccinia striiformis, P. graminis, P. hordei) , black rust (Puccinia graminis), cedar-apple rust, rust (Phragmidium spp.), grape rust (Phakopsora ampelopsidis), Leaf rust (Puccinia triticina triticia), pear rust (Gymnosporangium haraeanum), southern rust (Puccinia polvsora), soybean rust (Phakopsora Phakopsora pachyrhizi), yellow rust (Puccinia striiformis tritici), onion rust (Puccinia allii), wheat rust (Puccinia striiformis ( Puccinia striiformis, P. graminis, P. recondita), white rust (Albugo sp., Puccinia horiana) and strobophyte rust (Clonartium ribicola) (Cronartium ribicola)).

Scab is caused by several fungi and bacteria and is characterized by hardening, overgrowth and sometimes fissures (crustaceous lesions) of fruit, tuber, leaf or stem tissue. Refers to the disease group. The leaves of diseased plants may die prematurely and fall off. Scab affects trees or plants such as apples, crabapples, cereals, cucumbers, peaches, pecans, photinia, potatoes and pyracantha. Fruit scab can be a major problem for apples and peaches, and potatoes are particularly susceptible to scab. Examples of scab include, but are not limited to, apple scab (Venturia inaequalis), citris scab (Elsinoe fawcetti), scab (Streptomyces scabies). scabies)), peach scab (Cladosporium carpophilum), scab (Venturia nashicola, V. pirina), potato scab (Spongospora subterranean differentiation type subterranea (Spongospora subterranean f.sp. subterranea) and white star disease (Elsinoe leucospira).

Smut is caused by a fungus and is characterized by the accumulation of fungal spores in soot-like masses called sporangia formed in rashes on seeds, leaves, stems, inflorescences and bulbs. Refers to the disease group. The sporangia usually crumble into a black powder that is easily dispersed by the wind. Many smut fungi invade plant embryos or seedlings, spread throughout the body, and emerge externally only when the plant approaches maturity. There is also smut, which is localized and actively infects growing tissue. Smut is most common in grasses, cereals and corn (maize), sugar cane as well as sorghum. Examples of smut include, but are not limited to, barley smut (Ustilago nuda), corn smut (Ustilago maydis, U. zeae), bare smut disease (Ustilago tritici) and forage smut (Tilletia caries).

Wilts are a group of plant diseases caused by numerous fungi and bacteria, characterized by permanent stunting, wilting and wilting, often followed by death of whole or parts of the plant. Point. Wilt-causing fungi include Fusarium and Verticillium species, and wilt-causing bacteria include Corynebacterium, Erwinia ( Erwinia), Pseudomonas and Xanthomonas species. Wilt affects over 500 species of trees, shrubs, vines, flowers, ornamental plants, vegetables, fruits, crops and weeds. Examples of wilt diseases include, but are not limited to, Fusarium wilt (Fusarium oxysporum), oak wilt (Ceratocystis fagacearum), Stewart wilt and Verticillium wilt ( Verticillium alboatrum, V. dahliae).

Pathogens whose populations may be controlled by the botanical pharmaceutical compositions, methods and/or uses disclosed herein include, but are not limited to, viruses, bacteria, fungi and nematodes. In addition, any stage of development can be controlled by the botanical compositions, methods and/or uses disclosed herein, including but not limited to eggs, larvae, Larvae, juveniles, pupae and living organisms are included.

The botanical pharmaceutical compositions, methods and/or uses disclosed herein can control populations of pathogens belonging to the kingdoms Monera, Fungi and Nematoda. In one embodiment, the botanical pharmaceutical compositions, methods and/or uses disclosed herein can control populations of pathogens belonging to the Eubacterial domain and Cyanobacteria (referred to as bacterial pathogens). In aspects of this embodiment, the botanical pharmaceutical compositions, methods and/or uses disclosed herein provide for Acidobacterium, Actinomycota, Aquifex, Armatimonas, Bacteroidetes, Caldisericum. Phylum, Chlamydia, Green Sulfur Bacteria, Green Non-Sulfur Bacteria, Chrysiogenes, Cyanobacteria, Deferibacter, Deinococcus-Thermus (Thermus), Dictyoglomus, Ercimicrobia, Fibrobacter , Firmicutes, Fusobacterium, Gematimonas, Lentisphaera, Nitrospira, Planktomyces, Proteobacteria, Spirochete, Synergistes, Tenerictes, Thermodesulfobacteria, Thermomicrobia, Thermotoga Or it can control a population of pathogens belonging to the Vercomicrobia phylum. A non-exhaustive list of specific genera of bacterial pathogens includes, but is not limited to, Agrobacterium, Corynebacterium, Cryphonectria, Erwinia, The genera are included in the genera Penicillium, Pseudomonas, Streptomyces and Xanthomonas. A non-exhaustive list of specific species of bacterial pathogens includes, but is not limited to, Agrobacterium tumefaciens, Cryphonectria parasitica, Erwinia amylovora, Penicillium Penicillium digitatum, P. Included are P. italicum, Pseudomonas syringae, Streptomyces scabies and Xanthomonas oryzae.

In another embodiment, the botanical compositions, methods and/or uses disclosed herein can control a population of pathogens belonging to the order Viridae. A non-exhaustive list of specific genera referred to as viral pathogens includes, but is not limited to, the genera Begomovirus, Potyvirus and Tobamovirus.

In another embodiment, the botanical pharmaceutical compositions, methods and/or uses disclosed herein control a population of pathogens belonging to the phyla Ascomycota, Basidiomycota, Deuteromycota or Zygomycota. be able to. A non-exhaustive list of specific genera referred to as fungal pathogens includes, but is not limited to, Albugo, Alternaria, Aphanomyces, Armillaria, Asterina , Asterinella, Basidiophora, Bipolaris, Botryosphaeria, Botrytis, Bremia, Ceratocystis, Cercospora ( Cercospora, Cercosporella, Cladosporium, Clitcybe, Cochliobolus, Colletotrichum, Corynespora, Cronarthium Cytospora, Diaporthe, Dibotryon, Diplocarpon, Diplotheca, Elsinoe, Erysiphe, Eutypella, Exobasi Exobasidium, Fusarium, Gloeocercospora, Gloeosporium, Glomerella, Gnomonia, Guignardia, Gymnosporangium Gymnosporangium, Microdochium, Microsphaera, Monilinia, Mycosphaerella, Nectria, Peronospora, Pestalothiopsis, Phakopsora, Phomopsis, Phragmidium, Filactinia Genus Phyllactinia, Phytophthora, Placosphaeria, Plasmodiophora, Plasmopara, Podosphaera, Pseudospora, Pseudospora Genus Puccinia, Pyrenophora, Pythium, Rhizoctonia, Schizothyrium, Sclerospora, Sclerotinia, Septoria, Spherocacea Sphaerotheca, Spongospora, Stigmea, Taphrina, Thanatephorus, Tilletia, Typhula, Uncinula, Urnula ( Urnula, Ustilago, Valsa, Venturia and Verticillium. A non-exhaustive list of specific species of fungal pathogens includes, but is not limited to, Alternaria alternata, Alternaria brassicicola, Alternaria japonica, Alternaria japonica, Alternaria Lunaria longipes, Alternaria solani, Aphanomyces cochlioides, Armillaria mellea, Bipolaris maydis, Botrytis quinellae (Botrytis quinellae) - Bremia lactucae, Ceratocystis fagacearum, Cercospora arachidicola, C. C. beticola, C. Oyster (C.kaki), C. C. personata, C. C. sojina, C. C. zeae-maydis, Cercosporella brassicae, Cladosporium carpophilum, C. C. fulvum, Clitcybe tabescens, Cochliobolus heterostrophus, C. fulvum. C. miyabeanus, Colletotrichum acutatum, C. C. capsici, C. Lagenarium (C. lagenarium), C. C. lindemthianum, C. lindemthianum. C. theaesinensis, C. C. tabacum, Corynespora cassiicola, Cronartium ribicola, Diaporthe phaseolorum, Dibotryon morbosum, Dibotryon morbosum (Diplocar) Elsinoe ampelina, E. E. fawcetti, E. E. leucospira, Erysiphe cichoracearum, E. E. graminis, E. E. pisi, Exobasidium reticulatum, Fusarium avenaceum, F. F. culmorum, F. F. gaminearum, F. gaminearum; F. oxysporum, F. oxysporum;ニバレ（Ｆ．ｎｉｖａｌｅ）、グレオセルコスポラ・ソルギー（Ｇｌｏｅｏｃｅｒｃｏｓｐｏｒａ ｓｏｒｇｈｉ）、グレオスポリウム・カキ（Ｇｌｏｅｏｓｐｏｒｉｕｍ ｋａｋｉ）、グロメレラ・シングラータ（Ｇｌｏｍｅｒｅｌｌａ ｃｉｎｇｕｌａｔａ）、ギグナルディア・ビドウェリィ（Ｇｕｉｇｎａｒｄｉａ ｂｉｄｗｅｌｌｉｉ）、ギムノスポランギウム・ハラエアヌム（Ｇｙｍｎｏｓｐｏｒａｎｇｉｕｍ ｈａｒａｅａｎｕｍ ), Microdochium nivale, Monilinia laxa, M. M. fructigena, M. M. fructicola, M. M. mali, Mycosphaerella melonis, M. M. nawae, Peronospora destructor, P. P. parasitica, P. P. sparsa, P. P. tabacina, Phakopsora ampelopsidis, P. P. pachyrhizi, Pomopsis juniperovora, P. P. vexans, Phytophtora cactorum, P. vexans. P. erythroseptica, P. P. infestans, P. P. parasitica, P. P. sojae, Plasmodiophora brassicae, Plasmopara viticola, Podosphaera leucotricha, Pyrenophora tritiki-repentis, Pyrenophora pedicola - Pseudoperonospora cubensis, Puccinia allii, P. P. graminis, P. P. hordei, P. P. horiana, P. P. polvsora, P. P. recondita, P. recondita; P. striiformis, P. P. triticina triticia, Rhizoctonia solani, Sclerotinia homeocarpa, S. S. rolfsii, S. S. sclerotiorum, Septoria chrysanthemi-indici, S. S. glycines, S. S. lycopersici, Sphaerotheca fuliginea, S. S. humuli, S. Pannosa (S. pannosa), Spongospora subterranean, Taphrina deformans, Thanatephorus cucumeris, Tilletia unnuulanes (Tiphura ithuanes) Uncinula necator, Ustilago maydis, U.S. U. nuda, U. U. tritici, U. U. zeae, Valsa ceratosperma, Venturia inaequalis, V. V. nashicola, V. V. pirina, Verticillium alboatrum and V. pirina. Includes V. dahlia.

In another embodiment, the botanical pharmaceutical compositions, methods and/or uses disclosed herein can control a population of pathogens belonging to the phylum Nematoda (Arctophyta). A non-exhaustive list of specific genera referred to as nematode pathogens includes, but is not limited to, Aphelenchoides, Belonolaimus, Criconemella, Dirofilaria, Ditilencus genera Ditylenchus, Heterodera, Hirschmanniella, Hoplolaimus, Meloidogyne, Onchocerca, Pratylenchus, Radophol and Radophol Included are the genus Rotylenchulus. Nematode pathogens A non-exhaustive list of specific species includes, but is not limited to, Dirofilaria immitis, Heterodera zeae, Meloidogyne incognita, Meloidogyne javanica, Meliophyllum Included are the chew (Onchocerca volvulus), the banana nematode (Radopholus similis) and the black nematode (Rotylenchulus reniformis).

The botanical compositions, methods and uses described herein are non-phytotoxic, have little potential to harm mammals or the environment, and are safe for use on plants or crops of high economic value. can be applied. Additionally, the botanical drug compositions, methods and uses described herein can be used indoors and outdoors and may soften, dissolve or otherwise adversely affect the surface to be treated. never. Finally, pathogens are not resistant to the botanical pharmaceutical compositions, methods and uses described herein.

Other aspects of the specification can be described as follows:
1. 1. A method of controlling a pathogen of a plant disease comprising applying an effective amount of a botanical composition to one or more plants infested with the pathogen and/or causing the pathogen to be exposed to the botanical composition. comprising, consisting essentially of, or consisting of applying an effective amount of a botanical drug composition to one or more loci on a plant to be controlled by application of the botanical drug composition the pathogen of the disease is adversely affected, the composition comprising, consisting essentially of, or consisting of the treated fermented microbial supernatant and one or more nonionic surfactants; and A method comprising no active enzymes or viable bacteria and having a pH of up to 5.0.
2. 1. A method of increasing plant growth and/or crop production comprising applying to one or more plants an effective amount of a botanical composition and/or applying one or more comprising, consisting essentially of, or consisting of applying an effective amount of a botanical drug composition to one or more locations where the plant is exposed; improved absorption, improved xylem sap flow through the xylem and improved photosynthetic product flow at the phloem, increased absorption of water, minerals and other nutrients from the soil, capillary action and/or static at the xylem. increased water pressure and/or increased synthesis of compounds and energy and/or destruction of one or more components that inhibit xylem sap flow and/or photosynthetic product flow, wherein the composition comprises a treated fermenting microorganism comprising, consisting essentially of, or consisting of a supernatant and one or more nonionic surfactants, and containing no active enzymes or viable bacteria, and having a pH of up to 5.0 ,Method.
3. 1. A method of maintaining or improving the efficiency of an irrigation system comprising, or substantially less than, applying an effective amount of a botanical composition to one or more pipes in a pipeline network of the irrigation system. or consisting of, application of the botanical composition to substantially remove one or more components that inhibit one or more pipeline networks of the irrigation system, and the composition to contain the treated fermenting microorganisms; comprising, consisting essentially of, or consisting of a supernatant and one or more nonionic surfactants, and containing no active enzymes or viable bacteria, and having a pH of up to 5.0 ,Method.
4. Use of an effective amount of a botanical composition for controlling plant diseases, the composition comprising or consisting essentially of a treated fermented microbial supernatant and one or more nonionic surfactants. A use which consists of or consists of and does not contain any active enzymes or viable bacteria and has a pH of at most 5.0.
5. Use of an effective amount of a botanical composition for increasing plant growth and/or crop production, the composition comprising a treated fermented microbial supernatant and one or more nonionic surfactants. A use which comprises, consists essentially of, or consists of and is free of any active enzymes or viable bacteria and has a pH of at most 5.0.
6. Use of an effective amount of a botanical composition for maintaining or improving the efficiency of an irrigation system, the composition comprising a treated fermented microbial supernatant and one or more nonionic surfactants; A use which consists essentially of or consists of and does not contain any active enzymes or viable bacteria and has a pH of at most 5.0.
7. The method of embodiments 1-3 or embodiments 4-6, wherein the treated fermented microbial supernatant is derived from fermented yeast supernatant, fermented bacterial supernatant, fermented mold supernatant, or any combination thereof. Use as indicated.
8. Fermented yeast supernatant containing Brettanomyces, Candida, Cyberlindnera, Cystofilobasidium, Debaryomyces, Dekkera, Fusarium ), Geotrichum, Issatchenkia, Kazachstania, Kloeckera, Kluyveromyces, Lecanicillium, Mucor, Neurospora , Pediococcus, Penicillium, Pichia, Rhizopus, Rhodosporidium, Rhodotorula, Saccharomyces, Schizophrenia Saccharomyces, Thrichosporon, Torulaspora, Torulopsis, Verticillium, Yarrowia, Zygosaccharomyces or Zygosaccharomyces 7. A method or use according to embodiment 6, which is obtained from a yeast species belonging to A.).
9. 7. The method or use of embodiment 6, wherein the fermented yeast supernatant is made from the yeast Saccharomyces cerevisiae.
10. Fermented bacterial supernatant containing Acetobacter, Arthrobacter, Aerococcus, Bacillus, Bifidobacterium, Brachybacterium , Brevibacterium, Barnobacterium, Carnobacterium, Corynebacterium, Enterococcus, Escherichia, Gluconacetobacter Gluconacetobacter, Gluconobacter, Hafnia, Halomonas, Kocuria, Lactobacillus, Lactococcus, Leuconostoc ), Macrococcus, Microbacterium, Micrococcus, Neisseria, Oenococcus, Pediococcus, Propionibacterium ( Propionibacterium, Proteus, Pseudomonas, Psychrobacter, Salmonella, Sporolactobacillus, Staphylococcus, Streptococcus , Streptomyces, Tetragenococcus, Vagococcus, Weissells or Zymomonas. method or use.
11. 7. A method or use according to embodiment 6, wherein the fermentation bacterial supernatant is obtained from a fungal species belonging to the genus Aspergillus.
12. The method of embodiments 1-3 or 7-11 or the use of embodiments 4-11, wherein the botanical drug composition comprises at least 35% by weight of treated fermented microbial supernatant.
13. The method of embodiments 1-3 or 7-12 or the use of embodiments 4-12, wherein the botanical composition comprises up to 95% by weight of treated fermented microbial supernatant.
14. The nonionic surfactant comprises, consists essentially of, or consists of a polyether nonionic surfactant, a polyhydroxyl nonionic surfactant and/or a nonionic biosurfactant , a method according to embodiments 1-3 or 7-13 or a use according to embodiments 4-13.
15. The polyhydroxyl nonionic surfactant comprises or consists essentially of sucrose esters, ethoxylated sucrose esters, sorbital esters, ethoxylated sorbital esters, alkylglucosides, ethoxylated alkylglucosides, polyglycerol esters or ethoxylated polyglycerol esters. 15. A method or use according to embodiment 14, consisting essentially of or consisting of.
16. Nonionic surfactants include amine oxides, ethoxylated alcohols, ethoxylated fatty alcohols, alkylamines, ethoxylated alkylamines, ethoxylated alkylphenols, alkylpolysaccharides, ethoxylated alkylpolysaccharides, ethoxylated fatty acids, ethoxylated fatty alcohols. or ethoxylated fatty amines or having the general ^formula H _{( OCH2CH2} ) _xOC6H4R1 , _{( OCH2CH2 ) xOR2} or H _{( OCH2CH2 )} ^{xOC (} O) _R2 , where x represents the number of moles of ethylene oxide attached to the alkylphenol and/or fatty alcohol or fatty acid, R ¹ represents a long-chain alkyl group and R ² represents a long-chain aliphatic group. A method according to embodiments 1-3 or 7-15 or a use according to embodiments 4-15 comprising, consisting essentially of or consisting of an agent.
17. 17. A method or use according to embodiment 16, wherein R ¹ is a C ₇ -C ₁₀ normal alkyl group and/or R ² is a C ₁₂ -C ₂₀ aliphatic group.
18. the nonionic surfactant is ethoxylated nonylphenol, ethoxylated octylphenol, ethoxylated ceto-oleyl alcohol, ethoxylated ceto-stearyl alcohol, ethoxylated decyl alcohol, ethoxylated dodecyl alcohol, ethoxylated tridecyl alcohol or ethoxylated castor oil; A method according to embodiments 1-3 or 7-17 or a use according to embodiments 4-17.
19. The method of embodiments 1-3 or 7-18 or the methods of embodiments 4-18, wherein the botanical drug composition comprises from about 1% to about 15% by weight of one or more nonionic surfactants. Use of.
20. 20. The method of embodiment 19, wherein the botanical drug composition comprises from about 5% to about 13% by weight of the one or more nonionic surfactants.
21. 21. The method of embodiment 20, wherein the botanical drug composition comprises from about 7% to about 11% by weight of the one or more nonionic surfactants.
22. The method of embodiments 1-3 or 7-21 or embodiment 4, wherein the botanical drug composition further comprises, consists essentially of, or consists of one or more anionic surfactants. -21.
23. 23. The method or use of embodiment 22, wherein the botanical drug composition comprises from about 0.5% to about 10% by weight of the one or more anionic surfactants.
24. 24. The method or use of embodiment 23, wherein the botanical drug composition comprises from about 1% to about 8% by weight of the one or more anionic surfactants.
25. 25. The method or use of embodiment 24, wherein the botanical drug composition comprises from about 2% to about 6% by weight of the one or more anionic surfactants.
26. The method according to embodiments 1-3 or 7-25 or the use according to embodiments 4-25, wherein the pH is up to 4.5.
27. 27. The method or use of embodiment 26, wherein the pH is from about 3.7 to about 4.2.
28. The method of embodiments 1-3 or 7-27 or the use of embodiments 4-27, wherein the botanical pharmaceutical composition is substantially non-toxic to humans, mammals, plants and the environment.
29. The method of embodiments 1-3 or 7-28 or the use of embodiments 4-28, wherein the botanical drug composition is biodegradable.
30. The method of embodiment 1 or 7-29 or the use of embodiment 4 or 7-29, wherein the effective amount of the botanical composition produces a detrimental effect on the pathogen to be controlled.
31. An effective amount of the botanical composition is about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90% of the pathogen to be controlled. %, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75%, at least 80% , at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90%, up to 91% %, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70% to about 85% , about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 31. The method of embodiment 30, wherein 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97%, or about 95% to about 99% are adversely affected. Or use.
32. The method of embodiment 1 or 7-31 or the use of embodiment 4 or 7-31, wherein the effective amount of the botanical composition kills the pathogen to be controlled.
33. An effective amount of the botanical composition is about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90% of the pathogen to be controlled. %, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75%, at least 80% , at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90%, up to 91% %, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70% to about 85% , about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 33. The method or use of embodiment 32, which kills 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97%, or about 95% to about 99% .
34. The method of embodiment 1 or 7-33 or the use of embodiment 4 or 7-33, wherein the effective amount of the botanical composition reduces the population size of the pathogen to be controlled. .
35. An effective amount of the botanical composition reduces the population size of the pathogen to be controlled by about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75% %, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90%, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70 % to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95% %, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, 35. According to embodiment 34, which is reduced by about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97%, or about 95% to about 99%. method or use of;
36. 36. The method of embodiment 1 or 7-35 or embodiment 4, wherein the effective amount of the botanical composition prevents the pathogen to be controlled from entering or infesting the locus or loci. Or the use according to 7-35.
37. The effective amount of the botanical composition is about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90% of the pathogen population, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, or about 99%; or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97% or at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90%, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95% , from about 93% to about 97%, from about 93% to about 99%, from about 95% to about 97%, or from about 95% to about 99%, from infesting or infesting one or more locations. A method or use according to embodiment 36.
38. The method of embodiment 2 or 7-29 or embodiments 5 or 7-29, wherein the effective amount of the botanical drug composition improves absorption of water, minerals and other nutrients from the soil by root hairs. Use as described.
39. An effective amount of the botanical composition reduces the absorption of water, minerals and other nutrients from the soil by root hairs by about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70 %, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90%, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80 %, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% % to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97%, or about 95% to about 99% improvement, A method or use according to aspect 38.
40. The method of embodiment 2, 7-29 or 38-39 or embodiment 5, 7-29 or 38, wherein the effective amount of the botanical drug composition improves xylem sap flow through the xylem. -39.
41. An effective amount of the botanical composition reduces xylem sap flow through the xylem by about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%. , about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96% %, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90% , up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or from about 70% to about 80%, from about 70% about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95% %, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93 % to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97%, or about 95% to about 99%. Or use.
42. The method of embodiment 2, 7-29 or 38-41 or embodiments 5, 7-29 or 38-41, wherein the effective amount of the botanical pharmaceutical composition improves phloem photosynthetic product flux. 41. Use according to 41.
43. an effective amount of the botanical composition reduces phloem photosynthetic product flux by about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%; about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96% , at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90%, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95% , about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% The method of embodiment 42, or use.
44. The method of embodiment 2, 7-29 or 38-43 or embodiment 5, 7, wherein the effective amount of the botanical drug composition increases absorption of water, minerals and other nutrients from the soil. -29 or 38-43.
45. An effective amount of the botanical composition reduces absorption of water, minerals and other nutrients from the soil by about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%. , about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95% %, at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89% , up to 90%, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75 % to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99% %, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97%, or about 95% to about 99%, embodiment 44 any method or use described in .
46. The method of embodiment 2, 7-29 or 38-45 or embodiments 5, 7-45, wherein the effective amount of the botanical drug composition increases capillary action and/or hydrostatic pressure in the xylem. 29 or 38-45.
47. An effective amount of the botanical composition reduces capillary action and/or hydrostatic pressure in the xylem by about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95% , at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90%, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99% , from about 93% to about 95%, from about 93% to about 97%, from about 93% to about 99%, from about 95% to about 97%, or from about 95% to about 99%. Any method or use described.
48. The method of embodiment 2, 7-29 or 38-47 or embodiments 5, 7-29 or 38-47, wherein the effective amount of the botanical drug composition improves transport of the material through the plant. 47. Use according to 47.
49. An effective amount of the botanical drug composition reduces transport of the material through the plant by about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96% , at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90%, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95% , about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% The method of embodiment 48, or use.
50. The method of embodiment 2, 7-29 or 38-49 or embodiments 5, 7-29 or wherein the effective amount of the botanical pharmaceutical composition increases synthesis of the compound and energy in the plant. Uses according to 38-49.
51. An effective amount of the botanical composition reduces the synthesis of compounds and energy in plants by about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%. %, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75% , at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90% %, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95% , about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 51. The method of embodiment 50, which increases by 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97%, or about 95% to about 99%. method or use.
52. The method of embodiments 2, 7-29, or 38-51 or embodiments, wherein the effective amount of the botanical pharmaceutical composition improves synthesis of compounds and energy required to maintain and continue plant growth. 5, 7-29 or 38-51.
53. An effective amount of the botanical pharmaceutical composition reduces the synthesis of compounds and energy necessary for the maintenance and continuation of plant growth by about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94% , at least 95%, at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90%, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90% , about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% - about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99% improvement; 53. A method or use according to embodiment 52.
54. Embodiment 2, wherein the effective amount of the botanical composition dissolves, disperses, or otherwise removes one or more components that inhibit xylem sap flow in the xylem. , 7-29 or 38-53 or the use according to embodiment 5, 7-29 or 38-53.
55. An effective amount of the botanical composition comprises about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, one or more components that inhibit xylem sap flow in the xylem. %, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88 %, up to 89%, up to 90%, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93% , about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99% dissolved; 55. A method or use according to embodiment 54 which disperses or otherwise removes.
56. Embodiment 2, wherein the effective amount of the botanical composition dissolves, disperses, or otherwise removes one or more components that inhibit photosynthetic product flow in the phloem, 7-29 or 38-55 or the use according to embodiments 5, 7-29 or 38-55.
57. An effective amount of the botanical composition comprises about 70%, about 75%, about 80%, about 85%, about 86%, about 87% one or more components that inhibit phloem photosynthetic product flow. , about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94% %, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88% , up to 89%, up to 90%, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90 %, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90 % to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99% dissolved or dispersed 57. A method or use according to embodiment 56, causing or otherwise removing.
58. Embodiments wherein the effective amount of the botanical composition dissolves, disperses, or otherwise removes one or more components that inhibit water flow within the pipeline network of the perfusion system. 3 or 7-29 or the use according to embodiments 6-29.
59. An effective amount of the botanical composition comprises about 70%, about 75%, about 80%, about 85%, about 86%, about 86%, about one or more components that inhibit water flow within the pipeline network of the perfusion system. 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99% or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90%, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70 % to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93% %, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97%, or about 95% to about 99% dissolved , dispersing or otherwise removing. 59. A method or use according to embodiment 58.
60. The method of embodiment 3, 7-29 or 58-59 or embodiments 6-29 or 58, wherein the effective amount of the botanical drug composition improves water transport throughout the pipeline network of the irrigation system. The use according to -59.
61. An effective amount of the botanical composition reduces water transport throughout the pipeline network of an irrigation system by about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75% %, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90%, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70 % to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95% %, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, 61, which improves from about 93% to about 95%, from about 93% to about 97%, from about 93% to about 99%, from about 95% to about 97%, or from about 95% to about 99%. method or use of;
62. The method of embodiments 1-3 or 7-61 or the use of embodiments 4-61, wherein the effective amount of the botanical pharmaceutical composition maintains and/or enhances the health and vigor of the plant.
63. An effective amount of the botanical composition maintains plant health and vigor and/or is about 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%. %, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%; or at least 70%, at least 75% , at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%; or up to 70%, up to 75%, up to 80%, up to 85%, up to 86%, up to 87%, up to 88%, up to 89%, up to 90% %, up to 91%, up to 92%, up to 93%, up to 94%, up to 95%, up to 96%, up to 97%, up to 98% or up to 99%; or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95% , about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 63. The method of embodiment 62, wherein the enhancement is 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97%, or about 95% to about 99%. method or use.
64. The effective amount of the botanical composition is about 1:50, about 1:75, about 1:100, about 1:125, about 1:150, about 1:175, about 1:200, about 1:225, about 1:250, about 1:275, about 1:300, about 1:325, about 1:350, about 1:375, about 1:400, about 1:425, about 1:450, about 1:475, about 1:500, about 1:525, about 1:550, about 1:575 or about 1:600; or at least 1:50, at least 1:75, at least 1:100, at least 1:125, at least 1:150 , at least 1:175, at least 1:200, at least 1:225, at least 1:250, at least 1:275, at least 1:300, at least 1:325, at least 1:350, at least 1:375, at least 1:400 , at least 1:425, at least 1:450, at least 1:475, at least 1:500, at least 1:525, at least 1:550, at least 1:575 or at least 1:600; or up to 1:50, up to 1: 75, max 1:100, max 1:125, max 1:150, max 1:175, max 1:200, max 1:225, max 1:250, max 1:275, max 1:300, max 1: 325, max 1:350, max 1:375, max 1:400, max 1:425, max 1:450, max 1:475, max 1:500, max 1:525, max 1:550, max 1: 575 or up to 1:600; or about 1:50 to about 1:100, about 1:50 to about 1:200, about 1:50 to about 1:300, about 1:50 to about 1:400, about 1 : 50 to about 1:500, about 1:50 to about 1:600, about 1:100 to about 1:200, about 1:100 to about 1:300, about 1:100 to about 1:400, about 1 : 100 to about 1:500, about 1:100 to about 1:600, about 1:200 to about 1:300, about 1:200 to about 1:400, about 1:200 to about 1:500, about 1 : 200 to about 1:600, about 1:300 to about 1:400, about 1:300 to about 1:500, about 1:300 to about 1:600, about 1:400 to about 1:500, about 1 :400 to about 1:600 or from about 1:500 to about 1:600 botanical composition to diluent ratio. Use as described.
65. The effective amount of the botanical composition is about 1:500, about 1:750, about 1:1000, about 1:1250, about 1:1500, about 1:1750, about 1:2000, about 1:2250, about 1:2500, about 1:2750, about 1:3000, about 1:3250, about 1:3500, about 1:3750, about 1:4000, about 1:4250, about 1:4500, about 1:4750, about 1:5000, about 1:5250, about 1:5500, about 1:5750, about 1:6000, about 1:7000, about 1:8000, about 1:9000 or about 1:10000; or at least 1:500 , at least 1:750, at least 1:1000, at least 1:1250, at least 1:1500, at least 1:1750, at least 1:2000, at least 1:2250, at least 1:2500, at least 1:2750, at least 1:3000 , at least 1:3250, at least 1:3500, at least 1:3750, at least 1:4000, at least 1:4250, at least 1:4500, at least 1:4750, at least 1:5000, at least 1:5250, at least 1:5500 , at least 1:5750, at least 1:6000, at least 1:7000, at least 1:8000, at least 1:9000 or at least 1:10000; or up to 1:500, up to 1:750, up to 1:1000, up to 1: 1250, max 1:1500, max 1:1750, max 1:2000, max 1:2250, max 1:2500, max 1:2750, max 1:3000, max 1:3250, max 1:3500, max 1: 3750, max 1:4000, max 1:4250, max 1:4500, max 1:4750, max 1:5000, max 1:5250, max 1:5500, max 1:5750, max 1:6000, max 1: 7000, up to 1:8000, up to 1:9000 or up to 1:10000; or about 1:500 to about 1:1000, about 1:500 to about 1:2000, about 1:500 to about 1:3000, about 1 : 500 to about 1:4000, about 1:500 to about 1:5000, about 1:500 to about 1:6000, about 1:500 to about 1:7000, about 1:500 to about 1:8000, about 1 : 500 to about 1:9000, about 1:500 to about 1:10000, about 1:1000 to about 1:2000, about 1:1000 to about 1:3000, about 1:10 00 to about 1:4000, about 1:1000 to about 1:5000, about 1:1000 to about 1:6000, about 1:1000 to about 1:7000, about 1:1000 to about 1:8000, about 1: 1000 to about 1:9000, about 1:1000 to about 1:10000, about 1:2000 to about 1:3000, about 1:2000 to about 1:4000, about 1:2000 to about 1:5000, about 1: 2000 to about 1:6000, about 1:2000 to about 1:7000, about 1:2000 to about 1:8000, about 1:2000 to about 1:9000, about 1:2000 to about 1:10000, about 1: 3000 to about 1:4000, about 1:3000 to about 1:5000, about 1:3000 to about 1:6000, about 1:3000 to about 1:7000, about 1:3000 to about 1:8000, about 1: 3000 to about 1:9000, about 1:3000 to about 1:10000, about 1:4000 to about 1:5000, about 1:4000 to about 1:6000, about 1:4000 to about 1:7000, about 1: 4000 to about 1:8000, about 1:4000 to about 1:9000, about 1:4000 to about 1:10000, about 1:5000 to about 1:6000, about 1:5000 to about 1:7000, about 1: 5000 to about 1:8000, about 1:5000 to about 1:9000, about 1:5000 to about 1:10000, about 1:6000 to about 1:7000, about 1:6000 to about 1:8000, about 1: 6000 to about 1:9000, about 1:6000 to about 1:10000, about 1:7000 to about 1:8000, about 1:7000 to about 1:9000, about 1:7000 to about 1:10000, about 1: in embodiments 1-3 or 7-63, wherein the ratio of botanical composition to diluent is from about 8000 to about 1:9000, from about 1:8000 to about 1:10000 or from about 1:9000 to about 1:10000. A method as described or a use as described in embodiments 4-63.
66. An effective amount of the botanical composition is about 0.0001%, about 0.0002%, about 0.0003%, about 0.0004%, about 0.0005%, about 0.0006%, about 0.0007% %, about 0.0008%, about 0.0009%, about 0.001%, about 0.002%, about 0.003%, about 0.004%, about 0.005%, about 0.006%, about 0.007%, about 0.008%, about 0.009%, about 0.01%, about 0.02%, about 0.03%, about 0.04%, about 0.05%, about 0 0.06%, about 0.07%, about 0.08%, about 0.09%, about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5 %, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9% or about 10%; or at least 0.0001%, at least 0.0002%, at least 0.0003%, at least 0.0004%, at least 0.0005%, at least 0. 0006%, at least 0.0007%, at least 0.0008%, at least 0.0009%, at least 0.001%, at least 0.002%, at least 0.003%, at least 0.004%, at least 0.005% , at least 0.006%, at least 0.007%, at least 0.008%, at least 0.009%, at least 0.01%, at least 0.02%, at least 0.03%, at least 0.04%, at least 0.05%, at least 0.06%, at least 0.07%, at least 0.08%, at least 0.09%, at least 0.1%, at least 0.2%, at least 0.3%, at least 0.3% 4%, at least 0.5%, at least 0.6%, at least 0.7%, at least 0.8%, at least 0.9%, at least 1%, at least 2%, at least 3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8%, at least 9% or at least 10%; or up to 0.0001%, up to 0.0002%, up to 0.0003%, up to 0.0004%, up to 0 .0005%, max 0.0006%, max 0.0007%, max 0.0008%, max 0.0009%, max 0.001%, max 0.002%, max 0.003%, max 0.004 %, max 0.005%, max 0.006%, max 0.007%, max 0.008%, max 0.00 9%, max 0.01%, max 0.02%, max 0.03%, max 0.04%, max 0.05%, max 0.06%, max 0.07%, max 0.08% , max 0.09%, max 0.1%, max 0.2%, max 0.3%, max 0.4%, max 0.5%, max 0.6%, max 0.7%, max 0.8%, up to 0.9%, up to 1%, up to 2%, up to 3%, up to 4%, up to 5%, up to 6%, up to 7%, up to 8%, up to 9% or up to 10% or about 0.0001% to about 0.0005%, about 0.0001% to about 0.001%, about 0.0001% to about 0.005%, about 0.0001% to about 0.01%, about 0.0001% to about 0.05%, about 0.0001% to about 0.1%, about 0.0001% to about 0.5%, about 0.0001% to about 1%, about 0.0001 % to about 5%, about 0.0001% to about 10%, about 0.0005% to about 0.001%, about 0.0005% to about 0.005%, about 0.0005% to about 0.01 %, about 0.0005% to about 0.05%, about 0.0005% to about 0.1%, about 0.0005% to about 0.5%, about 0.0005% to about 1%, about 0 .0005% to about 5%, about 0.0005% to about 10%, about 0.001% to about 0.005%, about 0.001% to about 0.01%, 0.001% to about 0.001% 0.001% to about 0.1%, 0.001% to about 0.5%, 0.001% to about 1%, 0.001% to about 5%, about 0.001% to about 10%, about 0.005% to about 0.01%, about 0.005% to about 0.05%, about 0.005% to about 0.1%, about 0.005% to about 0.5 %, from about 0.005% to about 1%, from about 0.005% to about 5%, from about 0.005% to about 10%, from about 0.01% to about 0.05%, from about 0.01% about 0.1%, about 0.01% to about 0.5%, about 0.01% to about 1%, about 0.01% to about 5%, about 0.01% to about 10%, about 0 0.05% to about 0.1%, about 0.05% to about 0.5%, about 0.05% to about 1%, about 0.05% to about 5%, about 0.05% to about 10% %, from about 0.1% to about 0.5%, from about 0.1% to about 1%, from about 0.1% to about 5%, from about 0.1% to about 10%, from about 0.5% Final concentration of about 1%, about 0.5% to about 5%, about 0.5% to about 10%, about 1% to about 5%, about 1% to about 10% or about 5% to about 10% A method according to embodiments 1-3 or 7-65 or a use according to embodiments 4-65 which is
67. comprising, consisting essentially of, or consisting of a treated fermented microbial supernatant and one or more nonionic surfactants, containing no active enzymes or viable bacteria, and having a pH of 5 A botanical pharmaceutical composition that is less than .0.
68. 68. The botanical composition according to embodiment 67, wherein the treated fermented microbial supernatant is derived from fermented yeast supernatant, fermented bacterial supernatant, fermented fungal supernatant, or any combination thereof.
69. Fermented yeast supernatant containing Brettanomyces, Candida, Cyberlindnera, Cystofilobasidium, Debaryomyces, Dekkera, Fusarium ), Geotrichum, Issatchenkia, Kazachstania, Kloeckera, Kluyveromyces, Lecanicillium, Mucor, Neurospora , Pediococcus, Penicillium, Pichia, Rhizopus, Rhodosporidium, Rhodotorula, Saccharomyces, Schizophrenia Saccharomyces, Thrichosporon, Torulaspora, Torulopsis, Verticillium, Yarrowia, Zygosaccharomyces or Zygosaccharomyces 69. The botanical composition according to embodiment 68, which is obtained from a yeast species belonging to A.).
70. 70. A botanical composition according to embodiment 69, wherein the fermented yeast supernatant is obtained from the yeast Saccharomyces cerevisiae.
71. Fermented bacterial supernatant containing Acetobacter, Arthrobacter, Aerococcus, Bacillus, Bifidobacterium, Brachybacterium , Brevibacterium, Barnobacterium, Carnobacterium, Corynebacterium, Enterococcus, Escherichia, Gluconacetobacter Gluconacetobacter, Gluconobacter, Hafnia, Halomonas, Kocuria, Lactobacillus, Lactococcus, Leuconostoc ), Macrococcus, Microbacterium, Micrococcus, Neisseria, Oenococcus, Pediococcus, Propionibacterium ( Propionibacterium, Proteus, Pseudomonas, Psychrobacter, Salmonella, Sporolactobacillus, Staphylococcus, Streptococcus , Streptomyces, Tetragenococcus, Vagococcus, Weissells or Zymomonas. A botanical pharmaceutical composition.
72. 72. A botanical composition according to embodiment 71, wherein the fermented bacterial supernatant is obtained from a bacterial species belonging to the genus Aspergillus.
73. 73. The botanical composition according to any one of embodiments 67-72, comprising at least 35% by weight of the treated fermented microbial supernatant.
74. 74. The botanical composition according to any one of embodiments 67-73, comprising up to 50% by weight of treated fermented microbial supernatant.
75. the nonionic surfactant comprises, consists essentially of, or consists of a polyether nonionic surfactant, a polyhydroxyl nonionic surfactant and/or a biosurfactant; A botanical pharmaceutical composition according to any one of embodiments 67-74.
76. The polyhydroxyl nonionic surfactant comprises or consists essentially of sucrose esters, ethoxylated sucrose esters, sorbital esters, ethoxylated sorbital esters, alkylglucosides, ethoxylated alkylglucosides, polyglycerol esters or ethoxylated polyglycerol esters. 76. A botanical drug composition according to embodiment 75, which essentially consists of or consists of.
77. Nonionic surfactants include amine oxides, ethoxylated alcohols, ethoxylated fatty alcohols, alkylamines, ethoxylated alkylamines, ethoxylated alkylphenols, alkylpolysaccharides, ethoxylated alkylpolysaccharides, ethoxylated fatty acids, ethoxylated fatty alcohols. or ethoxylated fatty amines or having the general ^formula H _{( OCH2CH2} ) _xOC6H4R1 , _{( OCH2CH2 ) xOR2} or H _{( OCH2CH2 )} ^{xOC (} O) _R2 , where x represents the number of moles of ethylene oxide attached to the alkylphenol and/or fatty alcohol or fatty acid, R ¹ represents a long-chain alkyl group and R ² represents a long-chain aliphatic group. 77. A botanical pharmaceutical composition according to any one of embodiments 67-76, comprising, consisting essentially of, or consisting of an agent.
78. 78. The botanical composition according to embodiment 77, wherein R ¹ is a C ₇ -C ₁₀ normal alkyl group and/or R ² is a C ₁₂ -C ₂₀ aliphatic group.
79. the nonionic surfactant is ethoxylated nonylphenol, ethoxylated octylphenol, ethoxylated ceto-oleyl alcohol, ethoxylated ceto-stearyl alcohol, ethoxylated decyl alcohol, ethoxylated dodecyl alcohol, ethoxylated tridecyl alcohol or ethoxylated castor oil; A botanical pharmaceutical composition according to any one of embodiments 67-78.
80. The botanical composition according to any one of embodiments 67-79, comprising from about 1% to about 15% by weight of the one or more nonionic surfactants.
82. The botanical composition according to embodiment 80, comprising from about 5% to about 13% by weight of one or more nonionic surfactants.
83. The botanical composition according to embodiment 81, comprising from about 7% to about 11% by weight of one or more nonionic surfactants.
84. The botanical composition according to any one of embodiments 67-83, further comprising, consisting essentially of, or consisting of one or more anionic surfactants. .
85. The botanical composition according to embodiment 84, comprising from about 0.5% to about 10% by weight of the one or more anionic surfactants.
86. The botanical composition according to embodiment 85, comprising from about 1% to about 8% by weight of one or more anionic surfactants.
87. The botanical composition according to embodiment 86, comprising from about 2% to about 6% by weight of one or more anionic surfactants.
88. 88. The botanical composition according to any one of embodiments 67-87, wherein the pH is up to 4.5.
89. 89. The botanical composition according to embodiment 88, wherein the pH is from about 3.7 to about 4.2.
90. 90. The botanical composition according to any one of embodiments 67-89, which is substantially non-toxic to humans, mammals, plants and the environment.
91. The botanical composition according to any one of embodiments 67-90, which is biodegradable.
92. A method of controlling a pathogen of a plant disease comprising applying an effective amount of a botanical composition as defined in any one of embodiments 67-91 to one or more plants infested with the pathogen and/or or comprising, consisting essentially of, or consisting of applying an effective amount of a botanical composition to one or more locations such that the pathogen is exposed to the botanical composition; A method, wherein application of a pharmaceutical composition for plant disease results in a detrimental effect on the pathogen of the plant disease to be controlled.
93. A method of increasing plant growth and/or crop production comprising applying an effective amount of a botanical drug composition as defined in any one of embodiments 67-91 and/or a botanical drug composition applying an effective amount of a botanical drug composition as defined in any one of embodiments 67-91 to one or more locations exposing one or more plants to the The application of a botanical pharmaceutical composition consists of or consists of improving uptake by root hairs, improving xylem sap flow through the xylem and improving photosynthate flow at the phloem, removing water, minerals from the soil. and other nutrient absorption, increased capillary action and/or hydrostatic pressure in xylem and/or increased synthesis of compounds and energy and/or inhibition of xylem sap flow and/or photosynthetic product flow or wherein destruction of multiple components is effected.
94. 92. A method of maintaining or improving the efficiency of an irrigation system comprising an effective amount of a botanical composition as defined in any one of embodiments 67-91 in one or more pipes in a pipeline network of the irrigation system. wherein application of the botanical composition comprises, consists essentially of, or consists of applying the one or more components that inhibit one or more pipeline networks of the irrigation system; fully removed, method.
95. Use of a botanical composition as defined in any one of embodiments 67-91 in an effective amount for controlling plant diseases.
96. Use of a botanical composition as defined in any one of embodiments 67-91 in an effective amount for increasing plant growth and/or crop production.
97. Use of a botanical composition as defined in any one of embodiments 67-91 in an effective amount to maintain or improve efficiency of an irrigation system.
98. Plant diseases include anthracnose, blight, canker, clubroot, damping-off, gall, flat blister, spot, powdery mildew, mold, mosaic virus, rot, rust, scab, The method of embodiment 1, 7-36 or 62-66 or the use of embodiment 4, 7-36 or 62-66 which is smut, wilt, or any combination thereof.
99. The method of embodiment 1, 7-36, 62-66 or 98 or embodiments 4, 7-36, 62-, wherein the pathogen is a viral pathogen, a bacterial pathogen, a fungal pathogen, a nematode pathogen, or any combination thereof. 66 or 98.

(Example)
The following non-limiting examples are provided for illustrative purposes only so as to provide a more complete understanding of the presently contemplated representative embodiments. These examples should not be construed as limiting any of the embodiments described herein, including those relating to botanical pharmaceutical compositions or methods or uses disclosed herein. be.

Example 1
Preparation of treated fermented yeast supernatant 1
To prepare the treated fermented yeast supernatant, a large jacketed mixing kettle is charged with about 1,000 L of hot water at a temperature of about 29°C to about 38°C for the fermentation reaction. To this water was added about 84.9 kg of black untreated cane molasses, about 25.2 kg of cane raw sugar and about 1.2 kg of magnesium sulfate. After thoroughly mixing the mixture, about 11.4 kg of saccharified malt and about 1.2 kg of baker's yeast were added and stirred a little. After incubating the mixture at about 26° C. to about 42° C. for about 3 days, there is a disappearance of the foaming reaction indicating substantially complete fermentation. At the end of fermentation, the yeast fermented composition is centrifuged to remove the "sludge" formed during the fermentation process. The resulting fermentation supernatant (approximately 98.59% by weight) was collected and sterilized by autoclaving. The treated fermented yeast supernatant can then be stored in liquid form for later use. Alternatively, the treated fermented yeast supernatant can be spray dried by methods known in the art to produce a dry powder. A dry powder form can also be stored and then used.

Example 2
Preparation of treated yeast supernatant 2
To prepare the treated fermented yeast supernatant, a large jacketed mixing kettle is charged with about 1,000 L of hot water at a temperature of about 29°C to about 38°C for the fermentation reaction. To this water was added about 42.5 kg of black untreated cane molasses, about 12.6 kg of cane raw sugar and about 1.2 kg of magnesium sulfate. After the mixture was thoroughly mixed, about 10.3 kg of saccharifying malt and about 1.2 kg of baker's yeast were added and stirred a little. After incubating the mixture at about 26° C. to about 42° C. for about 3 days, there is a disappearance of the foaming reaction indicating substantially complete fermentation. At the end of fermentation, the yeast fermentation culture is centrifuged to remove the "sludge" formed during the fermentation process. The resulting fermentation supernatant (approximately 98.59% by weight) was collected and autoclaved. The treated fermented yeast supernatant can then be stored in liquid form for later use. Alternatively, the treated fermented yeast supernatant can be spray dried by methods known in the art to produce a dry powder. A dry powder form can also be stored and then used.

Example 3
Preparation of treated yeast supernatant 3
To prepare the treated fermented yeast supernatant, a large jacketed mixing kettle is charged with about 1,000 L of hot water at a temperature of about 29°C to about 38°C for the fermentation reaction. To this water was added about 21.3 kg of black untreated cane molasses, about 6.3 kg of cane raw sugar and about 1.2 kg of magnesium sulfate. After the mixture was thoroughly mixed, about 9.3 kg of saccharified malt and about 1.2 kg of baker's yeast were added and stirred a little. After incubating the mixture at about 26° C. to about 42° C. for about 3 days, there is a disappearance of the foaming reaction indicating substantially complete fermentation. At the end of fermentation, the yeast fermentation culture is centrifuged to remove the "sludge" formed during the fermentation process. The resulting fermentation supernatant (approximately 98.59% by weight) was collected and autoclaved. The treated fermented yeast supernatant can then be stored in liquid form for later use. Alternatively, the treated fermented yeast supernatant can be spray dried by methods known in the art to produce a dry powder. A dry powder form can also be stored and then used.

Example 4
Preparation of a botanical composition To prepare a botanical composition, 1,000 L of treated fermented yeast supernatant in a large jacketed mixing kettle and 1 hot sterile water (about 60° C. to about 65° C.). ,000 L was added. To this mixture about 168.8 kg of linear secondary alcohol ethoxylate TERGITOL™ 15-S-7, about 168.8 kg of linear secondary alcohol ethoxylate TERGITOL™ 15-S-5, alkyl About 67.5 kg of DOWFAX™ 2A1 of diphenyl oxide disulfate and about 67.5 kg of TRITON™ H-66 of phosphoric acid polyether ester were added. This mixture was mixed well until it became a solution. Water was then added to bring the volume to about 4,500 L and stirred until completely mixed. The pH of the resulting botanical composition was adjusted with phosphoric acid to about 3.7 to about 4.2. The pH adjusted botanical composition was then filter sterilized to remove any microbial contamination.

The composition was found to be non-irritating and non-toxic to skin tissue and could be stored cold for several months without any appreciable loss or deterioration of efficacy.

DOWFAX™ 2A1 can be replaced by anionic biosurfactants such as STEPONOL® AM30-KE of ammonium lauryl sulfate, STEPONOL® EHS of sodium 2-ethylhexyl sulfate, or combinations thereof. .

Optionally, the resulting botanical composition is then added with about 1% by weight sodium benzoate, about 0.01% by weight imidazolidinyl urea, about 0.15% by weight diazolidinyl urea, about 0.25% by weight It may be mixed with preservatives or stabilizers such as calcium chloride. Add sodium benzoate, imidazolidinyl urea, diazolidinyl urea and calcium chloride with continued stirring. The temperature of the mixture is then gradually raised to 40° C. and the mixture is continuously stirred. The temperature is maintained at about 40° C. for about 1 hour to ensure that all components of the mixture are dissolved. The mixture is then cooled to about 20°C to about 25°C. The pH of the resulting botanical composition was adjusted with phosphoric acid to about 3.7 to about 4.2. The pH adjusted botanical composition was then filter sterilized to remove any microbial contamination.

Example 5
Preparation of Botanical Composition To prepare a botanical composition, a large jacketed mixing kettle was charged with 850 L of hot, sterile water (about 60° C. to about 65° C.). To this water is added approximately 7.62 g of dry powder of treated fermented yeast supernatant, approximately 37.5 kg of TERGITOL™ 15-S-7 linear secondary alcohol ethoxylate, and TERGITOL linear secondary alcohol ethoxylate. 15-S-5, about 15.0 kg of DOWFAX™ 2A1 of alkyldiphenyl oxide disulfate and about 25.0 kg of TRITON™ H-66 of phosphoric acid polyetherester were added. . This mixture was mixed well until it became a solution. Water was then added to bring the volume to about 1,000 L and stirred until completely mixed. The pH of the resulting botanical composition was adjusted with phosphoric acid to about 3.7 to about 4.2. The pH adjusted botanical composition was then filter sterilized to remove any microbial contamination.

Optionally, the resulting botanical composition is then added with about 1% by weight sodium benzoate, about 0.01% by weight imidazolidinyl urea, about 0.15% by weight diazolidinyl urea, about 0.25% by weight It may be mixed with preservatives or stabilizers such as calcium chloride. Add sodium benzoate, imidazolidinyl urea, diazolidinyl urea and calcium chloride with continued stirring. The temperature of the mixture is then gradually raised to 40° C. and the mixture is continuously stirred. The temperature is maintained at about 40° C. for about 1 hour to ensure that all components of the mixture are dissolved. The mixture is then cooled to about 20°C to about 25°C. The pH of the resulting botanical composition was adjusted with phosphoric acid to about 3.7 to about 4.2. The pH adjusted botanical composition was then filter sterilized to remove any microbial contamination.

The composition was found to be non-irritating and non-toxic to skin tissue and could be stored cold for several months without any appreciable loss or deterioration of efficacy.

DOWFAX™ 2A1 can be replaced by anionic biosurfactants such as STEPONOL® AM30-KE of ammonium lauryl sulfate, STEPONOL® EHS of sodium 2-ethylhexyl sulfate, or combinations thereof. .

In place of the dry powder of treated fermented yeast supernatant disclosed in Examples 1-3, commercially available treated fermenters including, for example, TASTONE® 154, TASTONE® 210 or TASTONE® 900 A dry powder of yeast supernatant can be used.

Example 6
Studies with Rice This example demonstrates the effect of the botanical compositions disclosed herein on plant growth and grain production in rice.

One study evaluated the effects of the botanical compositions disclosed herein on rice seed germination, seedling height and root length. Seeds of rice cultivar Oryzica 1 were divided into three groups of 100 seeds, bagged, and the bags were filled with the following solutions: 1) distilled water (control); 2) a botanical composition as disclosed herein. 2% solution of (92% treated fermentation supernatant and 8% surfactant); and 3) a botanical drug composition disclosed herein (90% treated fermentation supernatant and 10% surfactant). Each group was pretreated for 2 minutes by immersion in one of the 2% solutions of Treated seeds were sown in sterile sand and then placed in a soil grower for 7-10 days to germinate. The germination effect was evaluated by determining the germination rate, seedling height and root length. This test was performed four times. The results showed that the treatment of seeds of rice seed cultivar Oryzica 1 did not affect the measured variables, namely germination rate, seedling height and root length.

Another study evaluated the effect of the botanical compositions disclosed herein on the growth of rice seedlings. Seedlings of rice cultivar Oryzica 1 were divided into groups of 20 seedlings and transplanted into planters containing pre-sterilized and appropriately fertilized soil to meet the nutritional requirements for good rice growth. Seedlings were divided into 3 groups of 5 planters. Seedlings were treated with 2 mL of one of the three solutions daily for 20 days. Group 1 was treated with distilled water (control); Group 2 was treated with a 2% solution of the botanical composition disclosed herein (92% treated fermentation supernatant and 8% surfactant); Group 3 was treated with a 2% solution of the botanical composition disclosed herein (90% treated fermentation supernatant and 10% surfactant). At the end of the 20-day period, data were obtained on seedling height, stem production and grain yield.

The results showed that treatment groups 1 and 2 had significant improvement compared to control group 1. For example, one rating I, the ratio of stem growth to panicle (cluster of loosely branched flowers) growth. Control group 1 was found to have a higher stem/panel ratio (15.4/12.2), more long stems with very short panicles, and lower grain production. Treatments 1 and 2 have more favorable stem/panel ratios (10.4/7.4 and 12.2/10.2, respectively), resulting in higher grain yields. It was found that treatment group 2 had a better stem/panel ratio than treatment group 1, resulting in long stems with sufficient panicles. Furthermore, among the resulting panicles, control group 1 contained 13.5% empty grains (not filled with rice grains), indicating sterility. Treatment group 2 had 6.4% empty kernels and treatment group 3 had 3.9% empty kernels. Thus, treatment with the botanical composition disclosed herein reduced plant sterility. Furthermore, in terms of grains per plant, treatment group 2 produced 14% more than control group 1, and treatment group 3 produced 64% more than control group 1. In addition, root growth was increased by 55% in treatment group 2 compared to control group 1, and by 104% in treatment group 3 compared to control group 1. In summary, significant benefits were observed when rice plants were treated with a 2% solution of the botanical composition disclosed herein.

Example 7
Tomato Studies This example demonstrates the effect of the botanical compositions disclosed herein on tomato plant growth and fruit production.

Tomato seedlings were grown in a greenhouse on soil consisting of 40% sand and 60% organic compost. No chemical fertilizers or urea were used, but cow manure was applied. Tomato seedlings were divided into two groups. The control group included seedlings that were drip irrigated daily with distilled water (control) for 6 months. The treatment group included seedlings drip-irrigated with a 1:400 dilution of the botanical composition disclosed herein (90% treated fermentation supernatant and 10% surfactant). Plants were evaluated monthly for 6 months. Plant growth and fruit production were evaluated.

The results showed that the treatment group had significant improvement compared to the control group. For example, the plants in the treatment group were stronger and tougher than the control group throughout the 6 month period. Additionally, the control group had a mortality rate of 30% in this study, while the treatment group showed a 100% survival rate. Furthermore, the plants in the treatment group still flowered and set fruit after 5 months, while the control group stopped flowering and seeding after 3 months. Finally, the final crop yield of the treatment group was more than double that of the control group. For example, the control group yielded 720 Kg of tomatoes, while the treatment group yielded 1,715 Kg of tomatoes. In summary, significant benefits were observed when tomato plants were treated with a 2% solution of the botanical composition disclosed herein.

A similar test was performed with parsley. The results showed that 5 times more parsley could be harvested from the treatment group treated with a 2% solution of the botanical drug composition disclosed herein than the control group treated with distilled water.

A similar test was conducted with eggplant. The results showed that 28% more eggplants could be harvested from the treatment group treated with a 2% solution of the botanical drug composition disclosed herein than the control group treated with distilled water.

Example 8
Tests with Olive Trees This example demonstrates the effect of the botanical compositions disclosed herein on olive tree growth and fruit production.

Olive trees grown in an orchard were divided into two groups. Control trees were drip irrigated with distilled water (control) for two seasons. The treatment groups were drip irrigated with a 1:400 dilution of the botanical composition disclosed herein (90% treated fermentation supernatant and 10% surfactant) for two seasons. Plants were evaluated monthly during the study period. Plant growth and fruit production were evaluated.

The results showed that the treatment group had significant improvement compared to the control group. For example, the treated group yielded about 35% to about 40% more olives than the control group. Even more surprisingly, the olive trees in the treated group set fruit every year, whereas the control group set fruit every other year. Finally, the flavor of the oil, especially the phenolics, was enhanced and thus the treated group had an improved olive tree oil flavor or quality compared to the control group.

A further test with a 1:1000 dilution of the botanical composition disclosed herein gave similar results.

Nearly identical results were observed with walnut and almond trees.

Example 9
Testing with Tobacco This example demonstrates the effect of the botanical compositions disclosed herein on tobacco growth and fruit production.

Tobacco seedlings were grown in the field. Tobacco seedlings were divided into two groups. Control groups included seedlings drip-irrigated daily with distilled water (control) for 6 months. The treatment group included seedlings drip-irrigated with a 1:400 dilution of the botanical composition disclosed herein (90% treated fermentation supernatant and 10% surfactant). After transplanting into the field, treatment groups included seedlings that were drip irrigated with a 1:800 dilution of a botanical composition disclosed herein. Plants were evaluated monthly for 6 months. Plant growth and fruit production were evaluated.

The results showed that the treatment group had significant improvement compared to the control group. For example, tobacco crops in the treatment group grew about 40% to about 50% more than the control group. In addition, the tobacco crops in the treatment group had higher root growth and higher seedling survival rate.

Almost the same results were observed with cannabis, although the tests were carried out in greenhouses.

Example 10
Tests for Plant Diseases This example demonstrates the efficacy of the botanical pharmaceutical compositions disclosed herein in treating plant diseases caused by the pathogens disclosed herein.

Using a spray bottle, roses with leaf spot caused by fungal infestation were treated with a 1:200 dilution of the botanical composition disclosed herein. After 1-2 weeks, no fungal infestation was observed.

Using a drip irrigation system, olive trees with blight caused by fungal infestation were treated with a 1:1000 dilution of the botanical composition disclosed herein. After 1-2 weeks, no fungal infestation was observed.

Using a drip irrigation system, olive trees with blight caused by bacterial infestation were treated with a 1:1000 dilution of the botanical composition disclosed herein. After 1-2 weeks, no bacterial infestation was observed.

Finally, although aspects of the present specification have been elucidated by reference to specific embodiments, it will be appreciated by those skilled in the art that these disclosed embodiments may convey the principles of the presently disclosed subject matter. It should be understood that this is to be understood as an example only. Accordingly, it should be understood that the subject matter of this disclosure is in no way limited to the particular compounds, compositions, articles, devices, methodologies, protocols and/or reagents, etc., described herein unless otherwise specified. should. Further, it will be appreciated by those skilled in the art that certain changes, modifications, permutations, alterations, additions, subtractions and subcombinations thereof may be made in accordance with the teachings herein without departing from the spirit of the specification. . Accordingly, the following appended "claims" and the claims inserted hereafter are to be construed to include all such alterations, modifications, permutations, alterations, additions, subtractions and subcombinations to their true spirit and shall be interpreted as being inclusive.

Certain embodiments of this invention are described herein, including the best mode known to the inventors for carrying out the invention. Of course, variations to these described embodiments will become apparent to those of ordinary skill in the art upon reading the above description. The inventor expects skilled artisans to employ such variations as appropriate, and intends for the invention to be practiced otherwise than as specifically described herein. . Accordingly, this invention includes all modifications and equivalents permitted by applicable law of the subject matter recited in the claims appended hereto. Moreover, any and all possible combinations of the above embodiments are encompassed by the present invention, unless otherwise stated herein or otherwise clearly contradicted by context.

Alternative embodiments, groupings of elements or steps of the invention are not to be construed as limiting. Each group member may be referred to or claimed individually or in any combination with other group members disclosed herein. It is anticipated that one or more members of a group may be included in or deleted from a group for reasons of convenience and/or patentability. In the event of any such inclusion or deletion, the specification is deemed to include the group as modified, thus satisfying any Markush group description used in the appended claims. .

Unless otherwise indicated, all numerical values used in the specification and claims expressing features, items, amounts, parameters, characteristics, periods of time, etc., are understood to be modified in all instances by the term "about." It should be. As used herein, the term “about” means that the feature, item, amount, parameter, property or period so modified is less than or equal to the numerical value of the feature, item, amount, parameter, property or period described. It is meant to include a range of up to 10 percent above and below. Accordingly, unless indicated to the contrary, the numerical parameters set forth in the specification and attached claims are approximations that may vary. For example, in mass spectrometry instruments, "about" as used in connection with the mass of an ion or the mass-to-charge ratio of an ion can be very small in determining the mass of a given analyte. The term refers to +/-0.50 atomic mass units. At least, and without attempting to limit the application of the doctrine of equivalents to the scope of the claims, each numerical value is at least calculated by taking into account the number of significant digits reported and applying conventional rounding methods. should be interpreted.

The term “may” or “can” is used in connection with embodiments or aspects of embodiments If so, another meaning of "may not" or "cannot" accompanies it. Thus, where this specification discloses that an embodiment or aspect of an embodiment may be included as part of the inventive subject matter, it also expressly implies any negative limitations or exclusive conditions and It is meant that aspects of the form or embodiment may not be included as part of the subject matter of the present invention. Similarly, when the term "optionally" is used in reference to an embodiment or aspect of an embodiment, such embodiment or aspect of the embodiment may be included as part of the inventive subject matter. or may not be included as part of the subject matter of this invention. The applicability of such negative limitations or exclusions is based on whether the claimed subject matter states such negative limitations or exclusions.

While the numerical ranges and numbers setting forth the broad scope of the invention are approximations, the numerical ranges and numbers set forth in the specific examples are reported as precisely as possible. Any numerical range or numerical value, however, inherently contains certain errors necessarily resulting from the standard deviation found in their respective testing measurements. When numerical ranges are recited herein, it merely serves as a way of omitting individual references to individual numerical values falling within the range. Unless otherwise stated herein, each individual numerical value in a numerical range is incorporated herein as if it were written individually herein.

The terms "a," "an," and "the" and similar designators used in connection with the description of the present invention (especially in connection with the claims that follow) are referred to herein as It should be construed to include both the singular and the plural unless explicitly stated or clearly contradicted by context. Further, terms that indicate the order of the elements described, such as "first," "second," "third," etc., are used to distinguish between elements unless otherwise stated; It does not express or imply that the number of elements is required or limited, nor does it imply any particular location or order of such elements. Any of the methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any examples or exemplary language (e.g., "such as") set forth herein is merely intended to facilitate understanding of the invention and is intended to assist in understanding the otherwise claimed invention. It is not intended to limit the scope. No language used in the specification should be construed as indicating any non-claimed element essential to the practice of the invention.

Whether the open-ended transitional term "comprising" (and equivalent open-ended transitional phrases such as including, containing, and having) was filed When used in a claim, whether added by amendment or not, it includes every element, limitation, step and/or feature specified alone or in combination with unclaimed subject matter. and its named elements, limitations and/or features are essential; however, other unspecified elements, limitations and/or features, in addition, still form constructs within the scope of the claims. It is possible. Certain embodiments disclosed herein may be used in a claim to use the closed-ended transitional phrase "consisting of" or "consisting essentially of" instead of or as an amendment to "comprising." can be further qualified by using When the closed-ended transitional phrase "consisting of" is used in a claim, whether as filed or added by amendment, it refers to an element, limitation, or step not specified in the claim. or exclude any feature. A closed-ended transitional phrase "consisting essentially of" defines the scope of a claim to the specified elements, limitations, steps and/or features, as well as any novel feature(s) underlying the claimed subject matter. ) to any other element, limitation, step and/or feature that does not materially affect the Thus, the meaning of the open-ended transitional phrase “comprising” includes the specifically recited elements, limitations, steps and/or features, as well as any optional additional elements, limitations, steps and/or features not specified. defined as including all The meaning of the closed-ended transitional phrase "consisting of" is defined as including only the elements, limitations, steps and/or features specifically recited in the claim, whereas the closed-ended transitional phrase "consisting of" is defined as including only the elements, limitations, steps and/or features specifically recited in the claim. "essentially of" means substantially affecting the elements, limitations, steps and/or features specifically recited in a claim and the underlying novel feature(s) of the claimed subject matter. are defined to include only those elements, limitations, steps and/or features that do not affect the Thus, the meaning of the open-ended transitional phrase "comprising" (and equivalent open-ended transitional phrases) is defined by the closed-ended transitional phrase "consisting of" or "consisting essentially of" as a limitation. Claimed subject matter is included. Thus, embodiments described or so claimed herein using the phrase "comprising" are expressly or inherently disambiguated with respect to the phrases "consisting essentially of" and "consisting of." is set forth in, enforceable and supported by

All patents, patent publications and other publications referenced and identified herein describe and disclose, for example, compositions and methodologies described in such publications that can be used in connection with the present invention. For this purpose, it is individually and expressly incorporated herein by reference in its entirety. These publications are provided solely for their disclosure prior to the filing date of the present application. In no respect should this be construed as an admission that the inventors are not entitled to antedate such disclosure by virtue of prior invention or otherwise. Any statement as to the date or representation as to the contents of these documents is based on the information available to the applicants and does not constitute an admission as to the correctness of the dates or contents of these documents.

Finally, the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the invention, which is defined solely by the claims. Accordingly, the invention is not limited to precisely that shown and described.

Claims (24)

A method for controlling pathogens of plant diseases, comprising:
The method comprises applying an effective amount of a botanical composition to one or more plants infested with a pathogen, and/or one or more plants such that the pathogen is exposed to the botanical composition. applying an effective amount of the botanical drug composition to the locus of
an effective amount of the botanical composition is an amount such that the concentration of the botanical composition in the plant and/or locus is from 0.001% to 10%;
the pathogen is an infectious pathogen,
application of the botanical composition results in an adverse effect on the pathogen to be controlled;
Said botanical drug composition comprises a treated fermented yeast supernatant obtained from the yeast Saccharomyces cerevisiae , one or more nonionic surfactants, no active enzymes, less than 5.0 has a pH of
Said treated fermented yeast supernatant may denature, kill, or otherwise denature, kill, or otherwise denature, kill, or otherwise remove active enzymes from remaining viable yeast, yeast and malt, and any other microorganisms present in the fermented yeast supernatant, or enzymes from another source. A method that is processed through an otherwise destructive processing step. 2. The method of claim 1, wherein the effective amount of the botanical composition is an amount such that the concentration of the botanical composition in the plant and/or the locus is between 0.001% and 1%. 2. The method of claim 1, wherein the effective amount of the botanical composition is an amount such that the concentration of the botanical composition in the plant and/or the locus is between 0.01% and 10%. Said treatment steps include boiling, autoclaving, ionizing radiation, or removing active enzymes from remaining viable yeast, yeast and malt and any other microorganisms present in the fermented yeast supernatant or enzymes from another source. , denaturing, killing or otherwise destroying any other sterilization process. A method according to any one of claims 1 to 4 , wherein the botanical drug composition comprises from 5% to 95% by weight of treated fermented yeast supernatant. A method according to any preceding claim, wherein the botanical drug composition comprises at least 35% by weight of the treated fermented yeast supernatant. A method according to any one of claims 1 to 6 , wherein the botanical composition comprises up to 90% by weight of the treated fermented yeast supernatant. The method of any one of claims 1-7 , wherein the nonionic surfactant comprises a polyether nonionic surfactant, a polyhydroxyl nonionic surfactant and/or a biosurfactant. wherein said polyhydroxyl nonionic surfactant comprises sucrose esters, ethoxylated sucrose esters, sorbital esters, ethoxylated sorbital esters, alkyl glucosides, ethoxylated alkyl glucosides, polyglycerol esters or ethoxylated polyglycerol esters. Item 9. The method according to item 8 . The nonionic surfactant is an amine oxide, an ethoxylated alcohol, an ethoxylated fatty alcohol, an alkylamine, an ethoxylated alkylamine, an ethoxylated alkylphenol, an alkylpolysaccharide, an ethoxylated alkylpolysaccharide, an ethoxylated fatty acid, an ethoxylated fat. alcohols or ethoxylated fatty amines or general formulas H _{( OCH2CH2} ) _xOC6H4R1 , H _{( OCH2CH2 ) xOR2} or H _{( OCH2CH2 )} ^{xOC ( O} ) _R2 wherein x represents the number of moles of ethylene oxide attached to the alkylphenol and/or fatty alcohol or fatty acid, ^R1 represents a long-chain alkyl group, and ^R2 represents a long-chain A method according to any one of claims 1 to 9 , comprising a non-ionic surfactant presenting an aliphatic chain group. 11. The method of claim 10 , wherein R ¹ is a C ₇ -C ₁₀ normal alkyl group and/or R ² is a C ₁₂ -C ₂₀ aliphatic group. The nonionic surfactant is ethoxylated nonylphenol, ethoxylated octylphenol, ethoxylated ceto-oleyl alcohol, ethoxylated ceto-stearyl alcohol, ethoxylated decyl alcohol, ethoxylated dodecyl alcohol, ethoxylated tridecyl alcohol or ethoxylated castor oil. The method according to any one of claims 1 to 11 , wherein A method according to any preceding claim, wherein the botanical composition comprises 1% to 15 % by weight of the one or more nonionic surfactants. 14. The method of claim 13 , wherein said botanical composition comprises 5% to 13% by weight of said one or more nonionic surfactants. 15. The method of claim 14 , wherein said botanical composition comprises 7% to 11% by weight of said one or more nonionic surfactants. A method according to any preceding claim, wherein the botanical composition further comprises one or more anionic surfactants. 17. The method of claim 16 , wherein said botanical composition comprises 0.5% to 10% by weight of said one or more anionic surfactants. 18. The method of claim 17 , wherein said botanical composition comprises from 1% to 8% by weight of said one or more anionic surfactants. 19. The method of claim 18 , wherein said botanical composition comprises 2% to 6% by weight of said one or more anionic surfactants. 20. The method of any one of claims 1-19 , wherein the pH is at most 4.5. 21. The method of claim 20 , wherein said pH is between 3.7 and 4.2. The method of any one of claims 1-21 , wherein the botanical composition further comprises an antimicrobial agent. The method according to any one of claims 1 to 22 , wherein said botanical pharmaceutical composition consists only of ingredients that are non-toxic to humans, mammals, plants and the environment. A method according to any one of claims 1 to 23 , wherein the botanical drug composition consists only of ingredients that are biodegradable.