JP2000327550A - Skin preparation for external use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin preparation for external use capable of preventing the production of lipid peroxides and inflammation, blackening and aging of skin and having excellent scavenging effects on active oxygen by including an extract of CAMU CAMU (botanical name: Myrciaria dubia) and an active oxygen scavenger. SOLUTION: This skin preparation for external use is obtained by including (A) an extract of CAMU CAMU in an amount of preferably 0.0001-5 wt.%, more preferably 0.001-3 wt.% expressed in terms of a dry solid and (B) an active oxygen scavenger in an amount of preferably 0.0001-5 wt.%, more preferably 0.001-3 wt.% expressed in terms of a dry solid. The ingredient A is prepared by carrying out extraction thereof from a fruit of the CAMU CAMU which is a plant belonging to the family Myrtaceae with an extracting solvent (e.g. ethyl alcohol) under condition of a low temperature or heating. Vitamin C (derivative), superoxide dismutase, mannitol, an extract of Scutellaria Radix, an extract of a mellisa, an extract of a rosemary and the like are preferably used.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to an external preparation for skin, and more particularly, to the production of lipid peroxide and the inflammation, darkening and aging of skin by containing a camcam extract and an active oxygen remover. The present invention relates to a skin external preparation having an excellent active oxygen removing effect which can be prevented.

[0002]

2. Description of the Related Art Conventionally, skin external preparations such as emulsions, creams, lotions, packs, cleansers, foundations, ointments, and dispersions have been used to prevent the formation of lipid peroxide and prevent skin irritation, blackening, and aging. Various medicinal ingredients have been added to these for the purpose of doing so. In recent years, as one of the causes of skin aging, the adverse effect of active oxygen on the living body has been cited. Active oxygen includes superoxide, singlet oxygen,
Hydroxy radicals, hydrogen peroxide, etc. are known to produce lipid peroxides and damage nuclei, proteins, and enzymes. There is. In addition, in terms of quantity, at least the accumulation of damage caused by active oxygen lowers the biological function, which is a cause of the so-called aging phenomenon. For this reason, various active oxygen removers have been conventionally developed.

[0003]

Further, there has been a demand for the development of an active oxygen scavenger having a higher effect. However, in consideration of application to a biological system, not only the effect but also the viewpoint of safety is considered. Must be fully satisfactory. In other words, it has an excellent active oxygen removal effect,
In addition, there has been a demand for the development of a suitable active oxygen remover for application to biological systems.

[0004]

Means for Solving the Problems The present inventors have studied the effect of an active oxygen scavenger formulated in a skin external preparation for the purpose of preventing the formation of lipid peroxide and the inflammation, darkening and aging of the skin. As a result of intensive studies to improve the present invention, the present inventors have found that when the cam cam extract and the active oxygen remover are combined, the action originally possessed by the active oxygen remover is sufficiently exhibited, and the present invention has been completed.

That is, the present invention provides an external preparation for skin containing the following components (A) and (B): (A) a camcam extract; and (B) an active oxygen remover.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The cam cam extract, which is the component (A) of the present invention, is obtained from the fruit of a cam cam (scientific name Myrcilia Dubia), a plant belonging to the family Myrtaceae.
Extract using an extraction solvent. The preparation method is not particularly limited, but, for example, extraction is performed at low temperature or at room temperature to heating using various suitable solvents.

As the extraction solvent, for example, one or more of water; lower monohydric alcohols such as methyl alcohol and ethyl alcohol; and liquid polyhydric alcohols such as glycerin, propylene glycol and 1,3-butylene glycol are used. be able to. As an example of a preferred extraction method, extraction is performed at a low temperature for 2 to 3 days using ethyl alcohol having a water content of 20 to 90% (v / v), followed by filtration, and the obtained filtrate is left for about 5 days. And aged,
There is a method of performing filtration again.

The content of the cam cam extract, which is the component (A) of the present invention, is preferably 0.0001 as a dry solid content.
To 5% by weight (hereinafter simply referred to as "%"), more preferably 0.001 to 3%. When the content of the camu cam extract is in this range, the effects of the present invention are better exhibited.

[0009] As the active oxygen removing agent which is the component (B) of the present invention, superoxide dismutase (SO
D), carotenoids such as mannitol, beta-carotene, astaxanthin, lutein, hydroquinone and its derivatives, bilirubin, cholesterol, tryptophan, histidine, quercetin, quercitrin, catechin and its derivatives, rutin and its derivatives, taurine, thiotaurine, soybean and Egg-derived phospholipids, glabridine, glabrene, liquiritin, isoliquiritin, retinol and its derivatives (retinol palmitate, retinol acetate, etc.), retinal and its derivatives,
Vitamin A such as dehydroretinal and derivatives thereof, thiamines (thiamine hydrochloride, thiamine sulfate),
Riboflavins (riboflavin, riboflavin acetate, etc.), pyridoxines (pyridoxine hydrochloride, pyridoxine dioctanoate, etc.), flavin adenine dinucleotide, cyanocobalamin, folic acids, nicotinic acids (nicotinamide, benzyl nicotinate, etc.), cholines, etc. Vitamin B and their derivatives, ascorbic acid and its derivatives (L-ascorbic acid, L-ascorbyl palmitate, L-ascorbyl dipalmitate, L-ascorbyl isopalmitate, L-diisopalmitate)
Ascorbyl, L-ascorbyl tetraisopalmitate, L-ascorbyl stearate, L-distearic acid
-Ascorbyl, L-ascorbyl isostearate,
L-ascorbyl diisostearate, myristic acid L
-Ascorbyl, L-ascorbyl dimyristate, L-ascorbyl isomyristate, L-ascorbyl diisomyristate, L-ascorbyl oleate, L-ascorbyl dioleate, L-2-ethylhexanoate
Ascorbyl, sodium L-ascorbate, potassium L-ascorbate,
L-ascorbic acid phosphate magnesium, L-
Ascorbic acid phosphate calcium, L-ascorbic acid phosphate aluminum, L-ascorbic acid sodium sulfate, L-ascorbic acid potassium sulfate, L-ascorbic acid magnesium sulfate, L-ascorbic acid calcium sulfate, L- Aluminum ascorbic acid sulfate, L
Vitamin Cs and their derivatives, such as sodium ascorbate, potassium L-ascorbate, magnesium L-ascorbate, calcium L-ascorbate, and aluminum L-ascorbate, ergocalciferol, cholecalciferol, dihydroxysta Vitamins such as nal and their derivatives, tocopherol and its derivatives (dl-α (β, γ) -tocopherol, dl-α-tocopherol acetate, nicotinic acid-dl-α-tocopherol, linoleic acid-dl-α-
Vitamin Es such as tocopherols, dl-α-tocopherol succinate), ubiquinones and their derivatives, glutathione and its derivatives, dibutylhydroxytoluene (BHT), butylhydroxyanisole (B
HA), gallic acid and derivatives thereof are preferred. These "derivatives" include the salts that can be formed.

Among these active oxygen scavengers, particularly preferred are vitamin C and its derivatives, superoxide dismutase (SOD), mannitol, astaxanthin, beta-carotene, dl-α-tocopherol, dibutylhydroxytoluene (BH
T) and butylhydroxyanisole (BHA).

Further, as the other component (B) of the present invention, preferred are the following active oxygen removers derived from animals, plants and microorganisms. Eggshell membrane extract, placenta extract of pigs, cows, etc., stevia extract, licorice extract, yeast extract, pentagon extract, seaweed extract, tolmentilla extract, arhat extract, ginkgo extract, reishi extract , Grape extract, calyx extract, green tea, black tea, tea extract such as oolong tea, bean tea extract, peony extract, gentian extract, hawthorn extract, rosemary extract, mocca extract, saxifrage extract, yashajitsu Extract, geno ginger extract, buttonpi extract, melissa extract,
Examples include parsley extract, dickopi extract, lettuce extract, cucumber extract, and metabolites of bifidobacteria.

Among these active oxygen scavengers, particularly preferred ones are an extract of pentagon, an extract of melissa,
Licorice extract, Saxifrage extract, Gentian extract, Rosemary extract.

The content of the active oxygen remover in the skin external preparation of the present invention is preferably 0.0001 to 5%, more preferably 0.001 to 3%. When an extract derived from animals, plants and microorganisms is used as the extract, the dry solid content may be within this range. When the active oxygen scavenger is in this range, when combined with the camcam extract, it does not affect the camcam extract in the preparation, and has better temporal stability, an effect of preventing skin aging, and improving skin roughness. The effect is obtained. That is, the generation of lipid peroxide due to the generation of active oxygen in the skin, the inflammation of the skin, the darkening,
It is extremely effective in preventing aging. These active oxygen removing agents can be used alone or in combination of two or more.

The external preparation for skin of the present invention can be prepared according to a conventional method, in which the essential components (A) and (B) are in various forms known as ordinary external preparations for skin.

Examples of the form of the external preparation for skin are not particularly limited, and include, for example, skin care cosmetics such as milky lotions, creams, lotions, packs, cleaning agents, makeup cosmetics such as lipsticks and foundations, and scalp cosmetics. , Ointments, dispersions, etc., can be used as external medicines, and there is no particular limitation on the form of the preparation, solid form, paste, mousse, gel, powder, solution, solubilization, emulsification, etc. Systems, powder dispersions, and multilayers.

In addition to the components (A) and (B), the external preparation for skin of the present invention usually contains cosmetics, quasi-drugs, and external medicines as long as the effects of the present invention are not impaired. Ingredients used in the formulation, i.e., water, oils, surfactants, metal soaps, gelling agents, powders, alcohols, water-soluble polymers, film-forming agents, resins, UV protection agents, clathrate compounds, antibacterial Agents, fragrances, deodorants, salts, pH regulators, cooling agents, animal / microbial extracts, plant extracts, blood circulation enhancers, astringents, antiseborrheic, whitening, anti-inflammatory, humectants, Anti-inflammatory agents, keratolytic agents, enzymes, hormones, vitamins and the like can be added.

[0017]

EXAMPLES Next, the present invention will be described in more detail with reference to Reference Examples, Test Examples and Examples, but the present invention is not limited thereto.

Reference Example 1 Production of cam cam extract Cam cam (Myrcilia, a fruit tree belonging to the family Myrtaceae)
To 30 g of dried fruit of Dubia), 100 ml of 80% (v / v) water-containing ethyl alcohol was added, extracted for 3 days at room temperature, and then filtered to obtain a cam cam extract (dry solids: 3). 0.0%).

REFERENCE EXAMPLE 2 Production of Orgon Extract A 10 g of Orgon (Scarabae root) is finely chopped, and 100 ml of absolute ethanol is added thereto.
Extracted for days and filtered to give a pentagon extract (dry solids 1.0%).

Test Example 1 Test for measuring superoxide removal activity The camcam extract and the active oxygen remover listed in Table 1 were used alone or in combination as a sample. After dilution with water, the superoxide removal activity was measured by the following measurement method. It was measured.

(Measurement method) A substrate solution [3.0 m] was added to 2.4 ml of 0.05 M sodium carbonate buffer (pH 10.2).
M xanthine (dissolved in 0.05 M sodium carbonate buffer)] 0.1 ml, 3.0 mM EDTA 0.1 ml,
0.15% (w / v) bovine serum albumin 0.1m
1, 0.75 mM nitro blue tetrazolium 0.1 m
and 0.1 ml of each test sample were mixed and left at 25 ° C. for 10 minutes. Next, 0.1 ml of an enzyme solution [xanthine oxidase solution (diluted to about 0.04 units / ml with purified water)] was added to start the reaction.
After incubation for 6 minutes, 6 mM CaCl ₂ 0.1
The reaction is stopped by adding ml. Next, the absorbance (A) at 560 nm is measured.

For the control, the absorbance (B) of a sample to which purified water was added instead of the test sample, and for each sample blank, 6
After adding 0.1 ml of mM CaCl ₂ and stopping the reaction, the absorbance (C) of the sample to which 0.1 ml of xanthine oxidase was added was measured, and the superoxide removal rate was calculated from the following equation. Table 1 shows the results.

[0023]

(Equation 1)

[0024]

[Table 1]

As is evident from the results in Table 1, the camcam extract had SOD-like activity even when used alone.
D. It was shown that when used in combination with the extract of Ogon, a synergistic effect was exhibited, and it was extremely effective in removing active oxygen.

Example 1 Cream A cream having the composition shown in Table 2 and the following production method (Product 1 of the present invention,
2 and Comparative products 1 to 4) were prepared, and their beautiful skin effect and skin aging prevention effect were examined. The results are also shown in Table 2.

[0027]

[Table 2]

(Production method) A. Components (1)-(7) and (10)-(12) are mixed and heated to 70 ° C. B. Ingredient (13) is mixed and heated to 70 ° C. C. B was added to A, mixed, cooled, and the components (8) and (9) were added and uniformly mixed to obtain a cream.

(Test method) 25 per test cream
A panel consisting of 15 women aged 55 to 55
For 12 weeks, after washing the face, an appropriate amount of the test cream was applied to the face. The beautiful skin effect and the skin aging prevention effect of the application were evaluated according to the following criteria.

(Evaluation Criteria) Beautifying Skin Effect <Evaluation> <Contents> Effective: The dullness of the skin became inconspicuous. Slightly effective: Skin dullness became less noticeable. Ineffective: No change from before use.

Skin Aging Prevention Effect <Evaluation> <Contents> Effective: Skin abrasion and gloss were improved. Slightly effective: Skin adhesion and gloss are slightly improved. Ineffective: No change from before use.

As shown in Table 2, the products of the present invention 1 and 2
By applying to the skin a cream that contains a camcam extract and an active oxygen remover represented by the above, the occurrence of `` dullness '' on the skin is prevented, the skin becomes beautiful, and the skin glue and gloss are improved. It was found to prevent skin aging.

Example 2 Lotion (Formulation) (%) (1) Glycerin 6.0 (2) 1,3-butylene glycol 5.5 (3) Polyoxyethylene (20EO) 1.5 Sorbitan mono Laurate (4) Ethyl alcohol 9.0 (5) Camcam extract * 1 10.0 (6) Mannitol * 2 0.5 (7) Preservative appropriate amount (8) Perfume appropriate amount (9) Purified water remaining amount * 1 Manufactured in Reference Example 1 * 2 Sigma

(Production method) Ingredients (3), (4), (7)
And (8) are mixed and dissolved. B. Components (1), (2),
(5), (6) and (9) are mixed and dissolved. C. A and B
Was mixed to obtain a lotion.

Example 3 Emulsion (Formulation) (%) (1) Polyoxyethylene (10EO) 1.0 Sorbitan monostearate (2) Polyoxyethylene (60EO) 0.5 Sorbitan tetraole Eate (3) Glyceryl monostearate 1.0 (4) Stearic acid 0.5 (5) Behenyl alcohol 0.5 (6) Squalane 8.0 (7) dl-α-tocopherol * 3 1.0 (8) Cam cam Extract * 1 3.0 (9) Astaxanthin * 2 0.02 (10) Preservative 0.1 (11) Carboxyvinyl polymer 0.1 (12) Sodium hydroxide 0.05 (13) Ethyl alcohol 5.0 (14) Remaining amount of purified water (15) Suitable amount of fragrance * 1 Produced in Reference Example 1 * 2 Sigma * 3 Eisai

(Production method) A. The components (11) to (14) are mixed by heating and kept at 70 ° C. B. Components (1) to (7), (9) and (10) are mixed by heating and maintained at 70 ° C. C. Add A to B, mix and emulsify uniformly. D. After cooling C, components (8) and (15) were added and mixed uniformly to obtain an emulsion.

Example 4 Ointment (Formulation) (%) (1) Stearic acid 18.0 (2) Cetanol 4.0 (3) Triethanolamine 2.0 (4) Glycerin 5.0 (5) Camcam extract * 1 2.0 (6) Licorice extract * 2 0.5 (7) Remaining purified water * 1 Produced in Reference Example 1 * 2 Maruzen Pharmaceutical Co., Ltd.

(Production method) A part of components (3), (4) and (7) are mixed by heating and kept at 75 ° C. B. Heat mix components (1) and (2) and maintain at 75 ° C. C. Add A slowly to B. D. While cooling C, the components (5) and (6) dissolved in the rest of the component (7) were added to obtain an ointment.

The lotion of Example 2, the emulsion of Example 3, and the ointment of Example 4 are all excellent in stability over time, and when applied to the skin, the skin will be improved in terms of radiance and luster, and the dullness of the skin will be improved. It prevented skin aging.

Example 5 Detergent (Formulation) (%) (1) Stearic acid 10.0 (2) Palmitic acid 8.0 (3) Myristic acid 12.0 (4) Lauric acid 4.0 (5) Oleyl Alcohol 1.5 (6) Refined lanolin 1.0 (7) Perfume appropriate amount (8) Preservative 0.1 (9) Potassium hydroxide 6.0 (10) Camu cam extract * 1 0.5 (11) Extract of ogre Product * 2 0.5 (12) Remaining purified water * 1 Manufactured in Reference Example 1 * 2 Manufactured in Reference Example 2

(Production method) Heat and mix components (9) and (12) and maintain at 70 ° C. B. Components (1) to (6) and (8) were mixed by heating.
Keep at ° C. C. A was added to B and maintained at 70 ° C. for a while. After the saponification reaction was completed, the mixture was cooled to 50 ° C., and components (7), (10) and (11) were added, and the mixture was cooled to obtain a cleaning agent.

Example 5 was a cleaning agent which was excellent in stability over time and applied to the skin to give a moisturizing and moisturizing effect when applied to the skin, and to provide a smooth and smooth skin.

Example 6 Pack (Formulation) (%) (1) Polyvinyl alcohol 20.0 (2) Ethyl alcohol 20.0 (3) Glycerin 5.0 (4) Kaolin 6.0 (5) Camcam extract * 1 1.0 (6) Rosemary extract * 3 1.0 (7) Saxifraga extract * 2 0.2 (8) Preservative appropriate amount (9) Perfume 0.05 (10) Purified water Remaining amount * 1 Reference Manufactured in Example 1 * 2 Ichimaru Falcos * 3 Maruzen Pharmaceutical

(Production Method) A. Mix components (1), (3), (4) and (10), heat to 70 ° C. and stir. B. Mix components (2), (8) and (9). C. B is added to A, mixed, cooled, and the components (5) to
(7) was uniformly dispersed to obtain a pack.

Example 6 was a pack which was excellent in stability over time and applied to the skin to improve the firmness and gloss of the skin and to prevent dullness of the skin and aging of the skin.

Example 7 Lip Cream (Prescription) (%) (1) Microcrystalline Wax 9.0 (2) Solid Paraffin 8.0 (3) Vaseline 15.0 (4) Cetyl Ethylhexanoate 15.0 (5 ) Liquid paraffin 8.0 (6) Castor oil balance (7) Camcam extract * 1 0.02 (8) Beta-carotene * 2 0.01 (9) BHT * 3 0.01 (10) Perfume appropriate amount * 1 Manufactured in Reference Example 1 * 2 Wako Pure Chemical Industries * 3 Sigma

(Production method) The components (1) to (6) are heated and dissolved at 100 to 110 ° C. B. At 90 ° C., the components (7) to (10) are added to A and mixed by stirring. C. B was cooled and molded to obtain a lip balm.

Example 7 was a lip cream which was excellent in stability over time, feeling in use and finish, kept lips smooth and prevented roughening.

Example 8 Powder Foundation (Formulation) (%) (1) Mica 50.0 (2) Titanium Oxide 15.0 (3) Nylon Powder 5.0 (4) Remaining Kaolin (5) Color Pigment 0 (6) paramethoxycinnamic acid 2.0 2-ethylhexyl (7) dimethylpolysiloxane 2.0 (8) squalane 3.0 (9) glyceryl trioctanoate 2.0 (10) petrolatum 1.0 (11) Camcam extract * 1 0.02 (12) Gentian extract * 2 0.5 (13) BHA * 3 0.01 (14) Perfume appropriate amount * 1 Manufactured in Reference Example 1 * 2 Manufactured by Technoble * 3 Sigma

(Production method) Mix components (1) to (5). B. Components obtained by heating and dissolving the components (6) to (14) are added, mixed and pulverized. C. B was filled in a metal plate and press-molded to obtain a powder foundation.

The powder foundation of Example 8 is
It was excellent in stability over time, feeling in use, and finish, and was applied to skin to suppress dullness and roughness of the skin and prevent skin aging.

[0052]

As described above, by combining the cam cam extract with the active oxygen remover, the original effects of the active oxygen remover can be fully exhibited. That is, it has an extremely high improvement and prevention effect against lipid peroxide generation, inflammation, blackening, aging and the like caused by active oxygen generation in the skin. Therefore, since the skin external preparation of the present invention can sufficiently exhibit the inherent performance of the active oxygen removing agent, it is extremely useful in beauty and medicine.

Continuation of the front page F-term (reference) 4C083 AA031 AA071 AA082 AA111 AA112 AB032 AB242 AB432 AB442 AC012 AC022 AC072 AC102 AC122 AC131 AC132 AC242 AC342 AC352 AC392 AC402 AC472 AC491 AC542 AC581 AC791 AC841 AC881 AD092 AD112 AD621 AD1 AD651 AD661 AD662 CC02 CC04 CC05 CC07 CC12 CC13 CC23 EE06 EE12 EE16

Claims (6)

[Claims] 1. An external preparation for skin, comprising the following components (A) and (B): (A) a camcam extract; and (B) an active oxygen remover. 2. An active oxygen scavenger comprising superoxide dismutase, mannitol, carotenoids, hydroquinone and its derivatives, bilirubin, cholesterol, tryptophan, histidine, quercetin, quercitrin, catechin and its derivatives, rutin and its derivatives, taurine, Thiotaurine, phospholipids, glabridine, glabrene, liquiritin, isoliquiritin,
Vitamin A and their derivatives, Vitamin B and their derivatives, Vitamin C and their derivatives, Vitamin D and their derivatives, Vitamin E and their derivatives, glutathione and its derivatives, dibutylhydroxytoluene, butyl The external preparation for skin according to claim 1, which is selected from hydroxyanisole, gallic acid and derivatives thereof. 3. The content of the active oxygen remover is 0.0001.
The external preparation for skin according to claim 2, wherein the amount is from 5 to 5% by weight. 4. The active oxygen scavenger is an eggshell membrane extract, a placenta extract, a stevia extract, a licorice extract, a yeast extract, a gougon extract, a seaweed extract, a tolmentilla extract, an arhat extract, and a ginkgo extract. Material, ganoderma extract, grape extract, calyx extract, tea extract, bean tea extract, peony extract, gentian extract, hawthorn extract, rosemary extract, mocca extract, saxifrage extract, yashajitsu extract 2. The external preparation for skin according to claim 1, wherein the preparation is selected from a substance, a ginger extract, a buttonpi extract, a melissa extract, a parsley extract, a zikkopi extract, a lettuce extract, a cucumber extract, and a metabolite of Bifidobacterium. 5. The external preparation for skin according to claim 4, wherein the content of the active oxygen remover is 0.0001 to 5% by weight as a dry solid content. 6. The skin external preparation according to any one of claims 1 to 5, wherein the content of the camcam extract is 0.0001 to 5% by weight as a dry solid content.