JP2000139334A - Stable and rapidly soluble protecting powder for egg of type dissolving at time of use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antimicrobial film-forming agent powder for eggs of the type dissolving at the time of use remarkably improved in fluidity and solubility of the powder and to provide a method for producing the powder. SOLUTION: This protecting agent powder for eggs is produced by (1) adding a mixture of an antimicrobial agent with a dispersing agent to an aqueous solution of polysaccharides, carrying out an emulsifying treatment and granulating the resultant liquid while spraying the liquid on a polymer having film-forming functions, (2) adding the mixture of the antimicrobial agent with the dispersing agent to the aqueous solution of the polysaccharides, conducting the emulsifying treatment, adding the polymer having the film-forming functions to the resultant liquid and spray-drying the prepared emulsion, (3) adding the mixture of the antimicrobial agent with the dispersing agent to the aqueous solution of the polysaccharides, performing the emulsifying treatment, adding the polymer having the film-forming functions to the resultant liquid and freeze-drying the prepared emulsion or (4) adding the mixture of the antimicrobial agent with the dispersing agent to the aqueous solution of the polysaccharides, carrying out the emulsifying treatment, spray-drying the obtained liquid and mixing the resultant powder with the polymer having the film-forming functions.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a stable and fast-acting, ready-to-use egg protective agent having improved fluidity and solubility of powder and a method for producing the same. More specifically, (1) a method in which a mixture of an antibacterial agent and a dispersant is added to an aqueous solution of a polysaccharide and emulsified, and a solution is sprayed onto a polymer compound having a film-forming function to granulate the solution; (3) a method of adding a mixture of an antibacterial agent and a dispersant to an aqueous solution of a polysaccharide and emulsifying the solution, and then adding a polymer compound having a film-forming function to the emulsion, and spray-drying the emulsion. A method in which a mixture of an antibacterial agent and a dispersant is added to an aqueous solution of a saccharide and emulsified, and a polymer compound having a film-forming function is added to the resulting emulsion to freeze-dry the emulsion; and (4) polysaccharides The aqueous solution of the fluidity obtained by adding a mixture of an antibacterial agent and a dispersing agent, and mixing the emulsified solution with a powder obtained by spray drying and a polymer compound having a film forming function, and the like, Stable, fast-acting, ready-to-use egg protectant powder with improved solubility Forefather process for the preparation of.

[0002]

2. Description of the Related Art Food poisoning and sometimes death by raw eggs contaminated with Salmonella have caused social problems, and recently the expiration date of chicken eggs has become obligatory under the direction of the Ministry of Health and Welfare. Most of the food poisoning due to Salmonella bacteria is derived from Salmonella enteritidis (SE bacteria), which attaches to chicken eggs via chicks, vectors, feeds, etc., and invades them. This Salmonella has a strong resistance in the natural environment and is very difficult to clean once the facility is contaminated. Therefore, as a second best measure, it has been desired to develop a safe and long-lasting antibacterial agent capable of completely controlling or sterilizing bacteria attached to eggs after laying eggs. In addition, there are many damages on the egg surface during the rainy season and during the rainy season, bacteria in the air adhere to the fine cracks on the egg surface, and harmful mold is generated on the egg surface. There was a cause. These mold eggs cannot be used not only for table eggs but also for commercial liquid eggs, and all of them are discarded, causing serious damage to producers.Therefore, the development of effective antifungal agents is also desired. Was. Furthermore, in recent years, the product liability system has been legislated in Japan, and in the field of antibacterial and antifungal agents, not only excellent antibacterial and antifungal properties, but also safety has been strictly required.

[0003] Conventionally, various antibacterial and / or antifungal agents have been proposed for the purpose of preventing disasters caused by the bacteria and mold. For example, (1) an inorganic antibacterial agent using silver or zinc, which is an antibacterial metal, (2) an organic antifungal agent such as TBZ, OPP, or diphenyl, or (3) an alcohol-based preparation for sterilizing the surface of an object. (4) Sustained combined eradication preparations for the purpose of continuously controlling germs by adding durable antibacterial components to alcohol preparations (5) Inexpensive hypochlorous acid showing strong bactericidal activity Chlorinated germicides represented by soda and the like are known.

[0004] However, most of the above-mentioned antibacterial and antifungal agents which have been used in the past contain almost no harmful components for foods, and they may be used improperly or used for a long time. Then, there is a high possibility of causing a serious secondary disaster. For example, the method using the inorganic antibacterial agent of the above (1) cannot be used in a form directly contacting food in terms of safety and hygiene. In addition, the silver agent easily discolors and alters components such as proteins in foods due to the mechanism of sterilization and antibacterial activity. (2) Organic antibacterial and antifungal agents are actually used by directly immobilizing them on the fruit surface using wax as a medium, but these drugs themselves have problems in terms of safety. , And cannot be easily removed by washing with water, etc.
There is a concern that the remaining drug will be taken into the body orally, and it has been raised as a post-harvest problem. (3) In alcohol-based preparations, the alcohol itself is volatile, and it is not possible to continuously control various germs. In addition, alcohol easily evaporates, and if used in large quantities, vaporized alcohol may enter the workplace, which may impair the health of workers engaged in the work. Further, depending on the material and properties of the target food, the food is deteriorated by the alcohol component, and therefore cannot be directly applied to the food. (4) Since the continuous complex disinfecting preparation does not contain a spreading agent, for example, in the case of a food having a smooth surface, effective spreading cannot be expected, and the active ingredient is peeled off by contact.

(5) Chlorine bactericides are inexpensive and have strong bactericidal activity, but are hardly safe compounds. In addition, their bactericidal action is transient, persistence is not guaranteed, and it is not possible to suppress the growth of bacteria partially remaining at the time of sterilization or bacteria attached during transport of eggs. In addition, since it is chlorine-based, there is a problem that economical and safety problems due to corrosion of used equipment and facilities cannot be avoided. In order to solve this problem, the present inventors have previously described a water-soluble polymer selected from the group consisting of polysaccharides, polyvinyl alcohol, polyvinyl pyrrolidone, polyvinyl methyl ether, carboxyvinyl polymer and sodium polyacrylate. An antibacterial and antifungal composition in which a relatively safe inorganic metal compound such as MgO having antibacterial and antifungal activity is uniformly dispersed in the form of fine powder in a viscous aqueous solution of a substance has been found and disclosed (International Publication WO98 / 28983). However, this was not a problem at the laboratory level, but not only proved to be poor in storage stability in practical use, but also made it difficult for the coating to become uniform and to make contact between the coatings during transportation. As a result, a part of the film is peeled off, and a part of the film is lost, resulting in a crack. Alternatively, a defect such as partial growth of bacteria or mold over time was found. Therefore, the present inventors have made further improvements, and as a result, comprise polysaccharides, polyvinyl alcohol, polyvinyl pyrrolidone, polyvinyl methyl ether, carboxyvinyl polymer, sodium polyacrylate, sodium oleate, vinyl acetate and morpholine fatty acid salts. Sustained antibacterial / antifungal coating agent in which an antibacterial agent having antibacterial / antifungal activity is suspended in an aqueous solution of a polymer compound having a film forming function selected from the group in the presence or absence of a surfactant. , And the growth and / or growth of fungi and molds attached to the surface of eggshell and the like containing the persistent antibacterial / antifungal coating agent as an active ingredient.
Alternatively, the present invention has succeeded in providing an inexpensive and useful persistent antibacterial / antifungal coating agent which can be controlled, and has already filed a patent application (Japanese Patent Application No. 10-197861). However, this persistent antibacterial and antifungal coating is a liquid,
Due to the weight, there are problems in safety, preservation, transportation and storage, and high costs due to manufacturing costs, packaging costs, transportation costs, etc. derived from the storage containers. In order to solve this problem, the present inventors have applied the above problems and social conditions to eggs, and are safe and have problems in storage, transportation, storage, and production derived from storage containers. Costs, packaging costs, transportation costs, etc. are not high costs, as a result of intensive studies on the development of a pre-melting egg protective agent, a powdered antibacterial agent containing a film-forming base, a dispersant, and an antibacterial agent as active ingredients. An egg protective agent having an antifungal, deodorizing effect or an egg shell enhancing effect has been found, and a patent application has already been filed (Japanese Patent Application No. 10-272196). However, the powder disclosed in Japanese Patent Application No. 10-272196 is obtained by kneading and mixing eutectic paraben in which lecithin is blended with sodium alginate powder, and has overcome the disadvantages of the liquid preparation such as stability. Oil quality (paraben) for all things
The powder was not very fluid and was not suitable for, for example, machine packaging. Also, when dissolving in a medium such as water at the time of use, the fluidity and dispersibility are insufficient, and occasionally, although slightly, it may become lump (insoluble lump), and it takes time to dissolve, There was still a problem where solubility had to be improved.

[0006]

SUMMARY OF THE INVENTION An object of the present invention is to provide a stable and quick-to-dissolve type egg-in-time protective agent having improved fluidity and solubility of powder, and a method for producing the same. I do. More specifically, (1) a method in which a mixture of an antibacterial agent and a dispersant is added to an aqueous solution of a polysaccharide and emulsified, and a liquid is sprayed onto a polymer compound having a film-forming function to granulate the solution.
(2) a method in which a mixture of an antibacterial agent and a dispersant is added to an aqueous solution of a polysaccharide, and an emulsion prepared by adding a polymer compound having a film-forming function to a liquid obtained by emulsification treatment is spray-dried;
(3) a method in which a mixture of an antibacterial agent and a dispersant is added to an aqueous solution of a polysaccharide and emulsified, and a polymer compound having a film-forming function is added to the emulsified liquid, and the resulting emulsion is freeze-dried; 4) A solution obtained by adding a mixture of an antibacterial agent and a dispersant to an aqueous solution of a polysaccharide and spray-drying the emulsified liquid is mixed with a polymer compound having a film-forming function. It is an object of the present invention to provide a stable and quick-to-use-type egg protective agent powder with improved fluidity and solubility that is improved when used and a method for producing the same.

[0007]

[Means for Solving the Problems]

The present inventors have conducted intensive studies in order to solve the above-mentioned problems, and as a result, have prepared a solution obtained by adding a mixture of an antibacterial agent and a dispersant to an aqueous solution of the polysaccharide of the present invention and emulsifying the solution. The present inventors have found that a powder-time-dissolving-type egg protective agent obtained by granulating while spraying a polymer compound having a membrane function can solve the above-mentioned problems. The present invention is based on such findings.

In other words, the present invention provides a stable and quick-to-use egg-type protective agent for use in the form of a time-soluble egg powder which is obtained by adding (1) a mixture of an antibacterial agent and a dispersant to an aqueous solution of a polysaccharide and emulsifying the mixture. A method of granulating while spraying a polymer compound having a film forming function,
(2) a method in which a mixture of an antibacterial agent and a dispersant is added to an aqueous solution of a polysaccharide, and an emulsion prepared by adding a polymer compound having a film-forming function to a liquid obtained by emulsification treatment is spray-dried;
(3) a method in which a mixture of an antibacterial agent and a dispersant is added to an aqueous solution of a polysaccharide and emulsified, and a polymer compound having a film-forming function is added to the emulsified liquid, and an emulsion prepared is freeze-dried;
(4) A method of adding a mixture of an antibacterial agent and a dispersant to an aqueous solution of a polysaccharide and spray-drying the emulsified liquid, and mixing the obtained powder with a polymer compound having a film-forming function. It is a stable and fast-acting, ready-to-use egg protective agent powder.

[0010] The pre-dissolved egg protective powder of the present invention can be stably stored for a long period of time, and can be rapidly and uniformly dissolved in a medium when used. The dissolution rate of the powder obtained by the present invention and the dissolution rate of the powder obtained by the production method of Japanese Patent Application No. 10-272196 are, for example, prepared in a 0.6% solution, in hot water under stirring. When compared under the condition of complete dissolution, the product of the present invention was completely dissolved in 5 minutes or less. In contrast, the conventional product required about 15 minutes.

The pre-dissolved egg-type protective agent of the present invention can be used as it is or by dissolving or suspending in an appropriate medium.
It can be effectively used as an antibacterial and antifungal agent for eggs having a shell such as chicken eggs.

The stable and fast-acting, ready-to-use type egg protective agent powder of the present invention can be produced by the method described below. (1) a method in which a mixture of an antibacterial agent and a dispersant is added to an aqueous solution of a polysaccharide and emulsified, and a liquid is emulsified and sprayed onto a polymer compound having a film forming function; A method of spray-drying an emulsion prepared by adding a polymer compound having a film-forming function to a liquid emulsified by adding a mixture of an antibacterial agent and a dispersant, (3) an aqueous solution of a polysaccharide, A method of freeze-drying an emulsion prepared by adding a polymer compound having a film forming function to a liquid emulsified by adding a mixture of an antibacterial agent and a dispersant, (4) an aqueous solution of a polysaccharide,
This is a method of mixing a powder obtained by adding a mixture of an antibacterial agent and a dispersant and emulsifying and spray-drying and granulating with a polymer compound having a film-forming function.

The above-mentioned fluidized-bed granulation, spray-drying, freeze-drying, mixing and the like are not particularly limited, and can be performed according to a conventional method.

As the polysaccharide used in the present invention, food-compatible thickening polysaccharides such as gum arabic, starch, dextrin and sodium CMC can be suitably used.
In the present invention, one or more of these may be used.

The film-forming base used in the present invention is a polymer compound having a film-forming function selected from the group consisting of polysaccharides, sodium polyacrylate, sodium oleate, vinyl acetate and morpholine fatty acid salts. It is. More specifically, for example, gum arabic, arabinogalactan, alginates such as sodium alginate, alginates such as propylene glycol alginate, casein, gati gum, carrageenan, karaya gum, carbanauba wax, sodium carboxymethylcellulose, candelilla wax, agar , Xanthan gum, chitosan, guar gum, gutta percha, whale wax, starch, glycol ester, crystalline cellulose, rice bran, sandalack, shellac, gellan gum, gelatin, tamarind seed polysaccharide, dammar gum, chicle gum, tragacanth gum, furcellan, pullulan, powdered cellulose, Thickening polysaccharides such as pectin, Peruvian balsam, beeswax, locust bean gum, cyclodextrin, sodium polyacrylate Unsalted, a polymer compound having a film-forming function of at least one of sodium oleate is selected from the group consisting of vinyl acetate and morpholine fatty acid salts. Preferred is sodium alginate. Sodium alginate, which is a water-soluble high molecular weight polysaccharide, can be said to be the most preferable substrate. In other words, they can form an extremely thin film on an object in a state in which the egg protecting agent of the present invention is dissolved or suspended, can continuously suppress the growth of harmful bacteria, and have a fluidity and a structure. It is a preferred substrate because it has a membrane function and is compatible with highly safe antibacterial and antifungal agents.

The dispersant includes an emulsifier used as a food additive. Among them, the neutral surfactant is preferable because it can be used without considering the influence and compatibility with the thickening polysaccharide used. More preferred are lecithin, sorbitan fatty acid esters, glycerin fatty acid esters, sucrose fatty acid esters, propylene glycol fatty acid esters, and the like, which are harmless to the human body. More preferably, it is lecithin. The amount of the dispersant used is not particularly limited, but preferably, when the powdered egg protective agent of the present invention is dissolved or suspended in water, the amount of the liquid coating agent is 0.0
It is an amount corresponding to 01% to 5% by weight.

As antibacterial agents, ethyl paraoxybenzoate (hereinafter referred to as ethyl paraben), propyl paraoxybenzoate (hereinafter referred to as propyl paraben), butyl paraoxybenzoate (hereinafter referred to as butyl paraben), and isopropyl paraoxybenzoate (hereinafter referred to as butyl paraben) Benzoic acid esters (hereinafter referred to as parabens) such as isobutyl paraben), isobutyl paraoxybenzoate (hereinafter referred to as isobutyl paraben), ε-polylysine, pectin decomposition product,
An antibacterial agent comprising at least one selected from the group consisting of dehydroacetic acid, protamine, chitosan, and fatty acids. These antibacterial agents are used as additives for preserving foods, and among them, parabens which can obtain a high effect are preferable. More preferred is a combination comprising butyl parahydroxybenzoate, isopropyl paraoxybenzoate, and isobutyl paraoxybenzoate.

The parabens used as the antibacterial agent in the present invention are substantially or almost insoluble in water, and it is usually difficult to prepare a high-concentration aqueous solution or emulsion, but at least 0.1% by weight, preferably 1% by weight. It is necessary that the content is not less than% by weight and the solubility is increased to be dissolved in the aqueous medium, or a state equivalent thereto (highly dispersed state emulsified with fine oil droplets). Some parabens are mixed in a specific mixing ratio, for example, butyl paraben, isobutyl paraben, and isopropyl paraben in a ratio of 3: 3: 4, and are carefully ground in the presence of a small amount of water to form a eutectic emulsion. Is reported to improve the solubility of the compound. However, this alone does not provide a stable and uniform emulsified state, and does not exhibit a sufficient antibacterial effect continuously due to poor adhesion. Unless a film agent is used, a high-concentration liquid and persistent antibacterial / antifungal coating agent cannot be obtained. However, in a preferred case of the present invention, when lecithin is added to the eutectic liquid, a high concentration (about 20% to about 40%) is obtained.
%) And a stable emulsion can be efficiently prepared.

In the powdery egg protective agent of the present invention, the amount of the parabens and the polysaccharide having a film-forming function is determined by the type, composition and amount of the parabens, or the type and usage of the polysaccharides used. Although it depends on the amount and the like and is not particularly limited, preferably, the amount is such that the composition containing oily parabens can be powdered, is obtained in a stable emulsified state at the time of preparation, and is formed when a film is formed. The range in which the alginate can sufficiently spread is suitable. For example, in the case of sodium alginate, 0.1 to 200 parts by weight, preferably 0.5 part by weight of sodium alginate per 1 part by weight of the parabens.
-20 parts by weight, more preferably 1-7 parts by weight.

[0020] The powdery ready-to-use type egg protective agent of the present invention can be added to water at the time of use and stirred almost within 5 minutes by a very simple method of adding to water. It can be a liquid-like solution.

The powdered egg protective agent of the present invention usually comprises 1
It can be dissolved or suspended in water of 1 to 10,000 times to prepare a persistent antibacterial / antifungal coating. Alternatively, a stock solution having a high concentration can be prepared, and this stock solution can be diluted and used according to the intended use. The dilution varies depending on the concentration of the stock solution of the antibacterial and antifungal coating agent of the present invention, the type of the film-forming base used, the type of the antibacterial agent, the combination of the film-forming base and the antibacterial agent, the preparation concentration, and the like, and is particularly limited. Not something.

The powdered egg protective agent of the present invention is stable for a long period of time. For example, it is stable even when stored at room temperature for several months without any problem in dissolution.

The method for dissolving or suspending the powder of the present invention on the surface of the object is not particularly limited, and the eggs may be immersed in this stock solution or diluent according to a conventional method, or The method of spraying the object can be appropriately selected.

Further, the liquid material adhering to the surface of the object is
According to an ordinary method, for example, it can be easily dried under natural drying or blowing. The film formed on the surface of the object was stable for a long time without decomposition. In addition, it did not peel off due to contact during transportation. By treating the powder of the present invention as described above, the powder of the present invention is not only an antibacterial and antifungal action, but also an enhancer of eggshell strength,
It can also be used as an eggshell deodorant.

The solubility of the ready-to-use type egg protective agent of the present invention,
Stability, antibacterial activity and antifungal activity are disclosed in Japanese Patent Application No. 10-27.
It can be confirmed by testing according to the method described in No. 2196. The present invention will be more specifically described with reference to the following Reference Examples, Examples, and Comparative Examples.

Reference Example 1 Production example of eutectic mixture: butyl parahydroxybenzoate (hereinafter butylparaben)
0.3 g, 0.3 g of parahydroxybenzoic acid isobutyl ester (hereinafter isobutylparaben) and 0.4 g of isopropylparahydroxybenzoate isopropyl ester or less (isopropylparaben) are placed in a glass container and shaken in a boiling water bath for 15 minutes. The mixture was heated with stirring to obtain a eutectic mixture as a transparent syrupy liquid.

Reference Example 2 To the eutectic mixture obtained in Reference Example 1 above, 0.05 g of lecithin was added, and the mixture was sufficiently stirred and mixed using a stirring rod to obtain a turbid yellow syrupy liquid. While adding 5 g of sodium alginate little by little to the mixture of parabens and lecithin, the mixture was stirred and mixed with a stirring rod to obtain a pale yellow uniform powder.

[0028]

EXAMPLE 1 1.0 g of sodium CMC, 20 g of gum arabic and 124 g of dextrin were dissolved in 150 g of water with stirring, and 100 g of the paraben eutectic solution obtained by the production method of Reference Example 1 was dissolved when completely dissolved. A mixture obtained by uniformly mixing 5.0 g of lecithin in advance was added and emulsified (primary emulsification) for 10 minutes with a homomixer to obtain a highly viscous milky liquid. Further add 100 g of water and then emulsify with a homomixer (2
Then, 500 g of an emulsion having a viscosity at room temperature of 300 cp (hereinafter referred to as emulsion A) was obtained. Next, 150 g of sodium alginate was put into a container of a fluidized-granulator (flow coater MINI, manufactured by Okawara Seisakusho), and then convection was started. After stabilizing at 80 ° C. heat and 40 ° C. waste heat, the above emulsion was added. 4g / min, average spray pressure 1.5kg
/ Cm ² (convection spraying for 20 seconds / 10 seconds off, back filter off pulse 1 second)
Fluid bed granulation was performed. Finally, 204 g of the above emulsion was sprayed on 150 g of sodium alginate to obtain 242 g of granules (96% recovery).

Example 2 Solubility test: 6.0 g of the powder obtained in Example 1 was added to 1000 m of hot water at 45 ° C.
l, and dissolve by stirring. Emulsion (hereinafter referred to as test solution A)
I got The dissolution time was within 5 minutes. On the other hand, the time required for treating the powder described in Reference Example 2 in the same manner as described above to obtain a completely dissolved milky substance (hereinafter referred to as test liquid B) was about 15 minutes.

Using the test solution A and the test solution B obtained by the method of the above-mentioned Example 2, the following tests of Examples 3 to 12 were performed. As a result, for the antibacterial activity test shown below,
The test test solution A in which the powder of the present invention was dissolved showed almost the same effect as the conventional test solution B.

Example 3 Formation of a film: A slide glass (2) having a surface polished with 0.1 ml of each of the test solution A and the test solution B obtained by the method of Example 2 was prepared.
(6 mm x 76 mm), spread uniformly, and air-dried. An extremely thin and uniform film could be formed on the treated surface after drying.

Example 4 Test of Antibacterial Activity against General Bacteria: Ten coated glass slides obtained by the method shown in Example 3 were prepared as test samples. On the other hand, as a control sample, 10 polished slide glasses were prepared per group. A diluted bouillon medium is applied to the surfaces of the test sample and the control sample, and stored in a high-humidity thermostat for 24 hours. Then, general bacteria are detected from the surface of the coated slide glass, and the number of bacteria is measured. Was done. As a result, 10 ⁴ to 10 ⁷ general bacteria were detected per slide from the slide glass of the control group. However, the number of bacteria per slide detected from the coated slide glass group was 10 ² or less. Some did not detect general bacteria. From this, it was confirmed that the film obtained by applying the solution A obtained by the method of Example 2 and drying had excellent antibacterial properties. More detailed test conditions are as shown below. Diluted broth medium concentration: 1/100 concentration. Coating amount: 0.
1 ml. Storage conditions: Stored in a high-temperature bath at a temperature of 30 ° C. and a relative humidity of 90% or more for 24 hours. General bacteria detection method: The slide glass surface coated with the diluted broth medium was wiped with absorbent cotton containing sterilized physiological saline, bacteria were washed out of the slide glass, and 48 hours by a dilution plate culture method using a standard agar medium. After the culture, the number of bacteria was measured. The detection limit is 10 bacteria per sheet.

Example 5 Antibacterial activity test against Escherichia coli: Using the coated slide glass obtained by the method shown in Example 3 as a test sample,
A test for confirming the antibacterial activity was carried out in the same manner as in Example 4 using the suspension in a broth medium diluted to 100 as an inoculum. It showed sufficient antibacterial activity.

Example 6 Antibacterial activity test against Salmonella A test for confirming the antibacterial activity was carried out in the same manner as in Example 5 using Salmonella as a test strain. As a result, sufficient antibacterial activity was shown.

Example 7 Test of antibacterial activity against bacteria adhering to eggs: A commercially available egg was added to the test solution A obtained in Example 2 for about 10 seconds.
After immersion treatment, the liquid was applied to the surface, and then naturally dried for one day. 50 chicken eggs thus treated were prepared. As a control group, 50 chicken eggs were prepared by immersing the washed egg instead of test solution A in water only. After the treatment, put 10 dried eggs in each egg pack,
The samples were stored in a high-temperature incubator at 30 ° C. keeping the humidity of 0% or more. These storage conditions are for creating conditions suitable for the propagation of bacteria on the egg surface. 3,6,10,15,20 from high temperature incubator storage
Ten days later, each of the treated eggs and the control washed eggs was taken out, and the number of general bacteria existing on the surface of the test eggs was measured.

The method for measuring general bacteria is as follows.
First, a sterile zippered plastic pack (85 × 12
(0 mm), add 10 ml of sterilized physiological saline thereto, shake carefully and sufficiently so as not to damage the test eggs, and wash out the bacteria on the eggshell surface. After 1 ml of physiological saline from which the bacteria were washed out was cultured at 35 ° C. for 48 hours by a pour plate method using a standard agar medium, the number of detected general bacteria was counted.

Example 8 Detection of Escherichia coli Group Using Dezoxycholate Agar Medium: In the test of Example 7, detection of Escherichia coli group using a desoxycholate agar medium was performed at the same time. For 20 days after the treatment, no bacteria belonging to the coliform group were detected.

Example 9 A similar test was carried out according to Example 7, except that the eggs were not washed (ie, the eggs were not washed at all) directly from an egg vendor.
Went against. As a result of the change in the number of bacteria per egg (no-washed egg) and the measurement of the number of general bacteria observed on the surface of the test eggshell during long-term storage at 30 ° C., the treatment with the above composition solution adhered to the surface of the unwashed egg. Reduce bacteria count and 30 after treatment
It was confirmed that bacterial regrowth was significantly prevented for 20 days under the storage conditions of ° C. Normally, unwashed eggs are known to be heavily stained by organic substances or various germs on the egg surface compared to commercially available chicken eggs, and are usually less effective by fungicides or antibacterial agents. It can be seen that the liquid is sufficiently effective even in such a case.

Example 10 Antifungal test: Eggs that are liable to develop mold, that is, 1) eggs whose eggshell surface is contaminated with chicken dung (hereinafter referred to as eggs with dung), 2) contamination by the contents of broken eggs Twenty pieces of the prepared eggs (hereinafter referred to as yolk stained eggs) and 3) chicken eggs (hereinafter referred to as cracked eggs) having cracks and exuding watery egg white were prepared. According to the procedure shown in Example 2, an aqueous solution of the antibacterial coating agent was prepared from the as-needed-dissolving antibacterial coating agent obtained in the same procedure as in Example 1, and each of an egg with feces, a yolk soiled egg, and a cracked egg was prepared. Ten of the twenty were treated with the antimicrobial coating agent solution of the present invention in the same manner as in Example 2. On the other hand, each of the remaining 10 eggs was washed only with water and prepared as a control test egg which was not treated with the solution of the antibacterial coating agent according to the present invention. Each of the test eggs prepared by this procedure was used in the same manner as in Example 7 for one egg.
0 pieces were put in a chicken egg pack and stored at 26 ° C. under a high humidity condition of 90% or more. Every day after storage, test chicken eggs were observed to check for the occurrence of mold.

In the untreated section of the egg with feces, small molds having white hyphae were observed around the feces attached after 4 days of storage, and as the number of days passed, the number of chicken eggs that produced similar molds increased. Around 7 days after storage, a slight black mold began to appear on some or all of the eggs. On the 15th day, mold development was confirmed on all 10 eggs.

In the untreated section of the yolk soiled egg, on the third day of storage, 1
Black or dark green mold was observed on the yolk marks (parts to which the yolk was attached) in individual chicken eggs, and the range of occurrence was increased according to the number of days elapsed, and the number of mold-producing chicken eggs increased. Mold development was confirmed in all ten eyes.

In the untreated section of the cracked eggs, the occurrence of black mold along the cracks was observed in two chicken eggs from 3 days after storage, and on the 7th day after storage, all the 10 eggs showed cracks in the eggs. Black mold developed on the surface. In addition, in the case of egg yolk and cracked eggs, as in the case of eggs with feces, the occurrence of black mold on the whole or part of the egg surface other than the contaminated part was observed around 7 days after storage. Was done.

On the other hand, in the treated plot, over the test period of 20 days, the occurrence of white mold from feces was observed in one chicken egg, and the appearance of white mold from feces was observed along two chicken eggs. Only the development of black mold occurred, and no mold development was observed in the yolk stained eggs throughout the test period. In addition, the occurrence of black mold which was partly or entirely observed on the eggs other than the contaminated part on the egg surface was not observed at all in the eggs in the untreated group.

Example 11 Eggshell Strength Enhancement Test: From the commercially available chicken eggs, 30 chicken eggs having blinding cracks were selected and used as test chicken eggs. Ten test liquids A
After immersion in the test solution, 10 were immersed in a solution prepared to have a concentration 10 times higher than that of the test solution A, and then naturally dried, and 10 were used as control groups without doing anything. These were carefully packed in metal round baskets and each was vibrated on a rotary sieve vibrator (1
00 rpm, 15 minutes).

Example 12 Deodorizing Effect Test: The chicken eggs treated with the test solution A had almost no off-flavor on the 7th day and a slight off-flavor on the 14th day. On the other hand, from the container storing untreated eggs at 30 ° C. and high humidity storage, an unusual smell peculiar to eggs was felt after about 7 days.

Examples 13 to 31 Eutectic mixed solutions of butylparaben, isobutylparaben and isopropylparaben having the compounding ratio described in Example 1 as antibacterial agents, xanthan gum and gum arabic instead of lecithin and sodium alginate as dispersants , Sodium carboxymethyl cellulose, hydroxypropyl cellulose, sodium alginate-xanthan gum,
Sodium alginate-sodium carboxymethyl cellulose, sodium alginate-hydroxypropyl cellulose, xanthan gum-gum arabic, xanthan gum-carboxymethyl cellulose sodium, xanthan gum-hydroxypropyl cellulose, arabic gum-sodium carboxymethyl cellulose, sodium carboxymethyl cellulose hydroxypropyl cellulose, etc. By combining the ratios, the in-use dissolved egg protective agents shown in Tables 1 and 2 below were prepared according to the method of Example 1 below.

[0047]

[Table 1]

[0048]

[Table 2]

Example 32 200 g of the emulsion A obtained by the production method described in Example 1
% G / w of a sodium alginate aqueous bath solution (700 g) was added, and 900 g of the emulsified solution was prepared using a mobile minor type spray drier (manufactured by Niro Japan Co., Ltd.) at an inlet temperature of about 170 ° C. and an outlet temperature of about 90 ° C. By spray drying, 140 g of powder was obtained (recovery rate: 58.3%).

Example 33 10% of the emulsion A obtained by the process described in Example 1 was added to 50 g of the emulsion A.
350 g of an aqueous solution of sodium alginate having a W / W concentration was added and mixed by stirring to obtain a high-viscosity milky liquid (hereinafter referred to as milky liquid B). 300 ml of this emulsion B is freeze-dried (manufactured by EYELA,
FD-80) to obtain 44.2 g of a light powder (recovery rate: 98.2%).

Example 34 300 g of the emulsion A obtained by the production method described in Example 1 was spray-dried using the above mobile minor type spray dryer at an inlet temperature of about 170 ° C. and an outlet temperature of about 90 ° C.
8.3 g were obtained. This powder and sodium alginate 12
3.6 g was sufficiently mixed to obtain 212 g of powder.

From the above results, the ready-to-use type egg protective agent of the present invention can be easily prepared in a solution state at the time of use. The antibacterial and antifungal effects are disclosed in Japanese Patent Application No. Hei 10-1978.
No. 61 and Japanese Patent Application No. 10-272.
The same effect as that obtained by dissolving the powder described in No. 196 in water was confirmed, and it was confirmed that the powder was useful as an antibacterial and antifungal agent.

The pre-dissolved egg protective agent of the present invention can be used as a coating material for eggshells by incorporating a natural or synthetic coloring agent and / or coloring agent which can be added to foods. It also includes a coloring agent which can confirm the uniformity of the coating at a glance as an indicator of coating adhesion as well as a coloring agent, and / or an in-use dissolving type protective agent for eggs which contains a coloring agent. In addition, by incorporating a natural or synthetic fragrance that can be added to foods in general, it also contains a fragrance that can be used for fragrance and prevention of off-flavor (fresh odor).

[0054]

According to the present invention, a method in which a mixture of an antibacterial agent and a dispersant is added to an aqueous solution of a polysaccharide and emulsified, is sprayed onto a polymer compound having a film forming function, and granulation is performed. The present invention has provided a stable and fast-acting egg-in-time protective agent for eggs to be dissolved and a production method thereof. INDUSTRIAL APPLICABILITY According to the present invention, it can be stored in a powder form, can be stably stored for a long period of time, can be rapidly and uniformly dissolved in a medium at the time of use, and can have fungi and mold attached to the surface of eggs, fruits, fresh foods, meats, etc. Inexpensive that can continuously suppress the growth of insects, can be used as a persistent antibacterial and antifungal coating agent that can be coated with a thin film, and is lightweight, has problems in storage, transportation, storage, and comes from storage containers Thus, a low-cost, ready-to-use-type egg protective agent that can save and reduce manufacturing costs, packaging costs, transportation costs, and the like can be provided. It is also useful as a ready-to-use eggshell strength enhancer and deodorant. The powder of the present invention can be stored stably for a long period of time, and can be rapidly and uniformly dissolved in a medium when used,
Egg shells such as chicken eggs can be easily coated, the growth of fungi and mold attached to the surface of the eggs can be suppressed continuously, and the strength of the eggshell can be reinforced, cracks can be prevented, and the deodorant effect can be expected. It is a highly soluble, ready-to-use antibacterial coating powder for eggs.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) C08J 3/12 CEP C08J 3/12 CEPZ 101 101 C08K 5/00 C08K 5/00 C08L 3/00 C08L 3 / 00 5/00 5/00 F-term (reference) 4B021 LA41 LW10 MC01 MK02 MK16 MK28 MP02 4B042 AC07 AD40 AE01 AG07 AH09 AK02 AK09 AP07 4F070 AA01 AA02 AA03 AC40 AC43 AC72 AC93 AC94 AE10 AE14 DA34 DA37 DC11 4J002 AB04 AB03 AB05 AB03 AB05 AB05 EH007 EH126 EL086 EW007 FD183 FD186 FD207 GE00

Claims (14)

[Claims] 1. A stable and rapid solution obtained by adding a mixture of an antibacterial agent and a dispersant to an aqueous solution of a polysaccharide and emulsifying the solution and spraying the solution onto a polymer compound having a film-forming function while granulating the mixture. Easily soluble egg protector powder for use. 2. An emulsion prepared by adding a mixture of an antibacterial agent and a dispersant to an aqueous solution of a polysaccharide and emulsifying the mixture, and then adding a polymer compound having a film-forming function to the emulsion, followed by spray drying. The resulting stable and fast-acting, ready-to-use egg type protective agent powder. 3. An emulsion prepared by adding a mixture of an antibacterial agent and a dispersant to an aqueous solution of a polysaccharide and emulsifying the mixture, and then adding a polymer having a film-forming function to the emulsion, and freeze-drying the emulsion. The resulting stable and fast-acting, ready-to-use egg type protective agent powder. 4. A method in which a mixture of an antibacterial agent and a dispersant is added to an aqueous solution of a polysaccharide, and a liquid obtained by spray-drying a solution emulsified is mixed with a polymer compound having a film-forming function. Stable and quick-acting egg-in-time protective agent powder obtained by the above method. 5. The dispersant according to claim 1, wherein the dispersant is at least one selected from the group consisting of lecithin, sorbitan fatty acid esters, glycerin fatty acid ester, sucrose fatty acid ester, and propylene glycol fatty acid ester. 2. The ready-to-use type egg protective agent powder according to the above. 6. The pre-dissolved egg protective agent powder according to claim 1, wherein the dispersant is lecithin. 7. The antibacterial agent is ethyl paraoxybenzoate,
Benzoic acid esters such as propyl paraoxybenzoate, butyl paraoxybenzoate, isopropyl paraoxybenzoate, isobutyl paraoxybenzoate, ε-polylysine, pectin degradation product, dehydroacetic acid, protamine, chitosan, and fatty acids are selected from the group consisting of: At least one
5. The pre-dissolved egg protective agent powder according to any one of claims 1 to 4, which is an antibacterial agent comprising at least one species. 8. The antibacterial agent is butyl paraoxybenzoate,
The in-use dissolving type egg protective agent powder according to any one of claims 1 to 4, which is a eutectic solution comprising isopropyl paraoxybenzoate and isobutyl paraoxybenzoate. 9. The pre-dissolved egg protective agent powder according to claim 1, wherein the polymer compound having a film-forming function is sodium alginate. 10. A method for producing the ready-to-use dissolved type egg protective agent powder according to any one of claims 1 to 9. 11. A ready-to-use egg shell strength reinforcing agent comprising the ready-to-use egg protective agent powder according to any one of claims 1 to 9. 12. A ready-to-use deodorant for eggshell, comprising the ready-to-use type egg protective agent powder according to any one of claims 1 to 9. 13. The pre-dissolved egg protective agent powder according to claim 1, which comprises a coloring agent and / or a coloring agent. 14. The pre-dissolved egg protective agent powder according to claim 1, which comprises a flavor.