GB1263765A - A method for the production of protease by cultivating bacteria - Google Patents
A method for the production of protease by cultivating bacteriaInfo
- Publication number
- GB1263765A GB1263765A GB4542269A GB4542269A GB1263765A GB 1263765 A GB1263765 A GB 1263765A GB 4542269 A GB4542269 A GB 4542269A GB 4542269 A GB4542269 A GB 4542269A GB 1263765 A GB1263765 A GB 1263765A
- Authority
- GB
- United Kingdom
- Prior art keywords
- protease
- production
- culture broth
- alkaline protease
- cultivating bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
1,263,765. Alkaline protease. GODO SHUSEI K.K. 18 Nov., 1969, No. 45422/69. Heading C3H. A process for the production of an alkaline protease having activity over an optimum pH range from 10 to 10À5 comprises cultivating strains of Bacillus licheniformis in a culture broth under aerobic conditions and isolating the protease from the culture broth. The enzyme may be extracted from the filtered or centrifuged medium by salting out, dialysis, ionexchange, column chromatography, gel filtration and finally acetone precipitation.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB4542269A GB1263765A (en) | 1969-11-18 | 1969-11-18 | A method for the production of protease by cultivating bacteria |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB4542269A GB1263765A (en) | 1969-11-18 | 1969-11-18 | A method for the production of protease by cultivating bacteria |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1263765A true GB1263765A (en) | 1972-02-16 |
Family
ID=10437155
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB4542269A Expired GB1263765A (en) | 1969-11-18 | 1969-11-18 | A method for the production of protease by cultivating bacteria |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB1263765A (en) |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5195181A (en) * | 1975-02-13 | 1976-08-20 | PUROTEAA ZENOSEIZOHO | |
EP0006638A2 (en) * | 1978-07-04 | 1980-01-09 | Novo Nordisk A/S | Microbial protease preparation suitable for admixture to washing compositions and process for preparing it |
GB2116561A (en) * | 1982-02-08 | 1983-09-28 | Showa Denko Kk | Novel alkaline protease and preparation method thereof |
US5904736A (en) * | 1995-04-28 | 1999-05-18 | Henkel Kommanditgesellschaft Auf Aktien | Cellulase-containing washing agents |
US6350728B1 (en) | 1996-12-11 | 2002-02-26 | Henkel Kommanditgesellschaft Auf Aktien (Kgaa) | Coated enzyme preparation with an improved solubility |
US6380140B1 (en) | 1996-04-20 | 2002-04-30 | Henkel Komm.Nditgesellschaft Auf Aktien | Enzyme granules containing phosphated starch |
US6979669B2 (en) | 2001-08-30 | 2005-12-27 | Henkel Kommanditgesellschaft Auf Aktien | Encapsulated active ingredient preparation for use in particulate detergents and cleaning agents |
US7300782B2 (en) | 2001-12-21 | 2007-11-27 | B.R.A.I.N. Biotechnology Research And Information Network Ag | Glycosyl hydrolases |
US7456143B2 (en) | 2002-12-20 | 2008-11-25 | Henkel Kommanditgesellschaft Auf Aktien (Henkel Kgaa) | Bleach-containing washing or cleaning agents containing a sulfate/silicate coated percarbonate |
US7569226B2 (en) | 2001-12-22 | 2009-08-04 | Henkel Kommanditgesellschaft Auf Aktien (Henkel Kgaa) | Alkaline protease from Bacillus sp. (DSM 14392) and washing and cleaning products comprising said alkaline protease |
US7803604B2 (en) | 2000-07-28 | 2010-09-28 | Henkel Ag & Co. Kgaa | Amylolytic enzyme extracted from Bacillus sp. A 7-7 (DSM 12368) and washing and cleaning agents containing this novel amylolytic enzyme |
US7888104B2 (en) | 2000-11-28 | 2011-02-15 | Henkel Ag & Co. Kgaa | Cyclodextrin glucanotransferase (CGTase), obtained from<I>Bacillus agaradherens<λ>(DSM 9948) and detergents and cleaning agents containing said novel cyclodextrin glucanotransferase |
CN117417922A (en) * | 2023-12-13 | 2024-01-19 | 北京科为博生物科技有限公司 | High-temperature-resistant alkaline protease and gene and application thereof |
-
1969
- 1969-11-18 GB GB4542269A patent/GB1263765A/en not_active Expired
Cited By (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5313708B2 (en) * | 1975-02-13 | 1978-05-12 | ||
JPS5195181A (en) * | 1975-02-13 | 1976-08-20 | PUROTEAA ZENOSEIZOHO | |
EP0006638A2 (en) * | 1978-07-04 | 1980-01-09 | Novo Nordisk A/S | Microbial protease preparation suitable for admixture to washing compositions and process for preparing it |
EP0006638A3 (en) * | 1978-07-04 | 1980-02-06 | Novo Industri A/S | Microbial protease preparation suitable for admixture to washing compositions and process for preparing it |
US4266031A (en) | 1978-07-04 | 1981-05-05 | Novo Industri A/S | Protease product of reduced allergenicity |
GB2116561A (en) * | 1982-02-08 | 1983-09-28 | Showa Denko Kk | Novel alkaline protease and preparation method thereof |
US4480037A (en) * | 1982-02-08 | 1984-10-30 | Showa Denko Kabushiki Kaisha | Alkaline protease and preparation method thereof |
US5904736A (en) * | 1995-04-28 | 1999-05-18 | Henkel Kommanditgesellschaft Auf Aktien | Cellulase-containing washing agents |
US6380140B1 (en) | 1996-04-20 | 2002-04-30 | Henkel Komm.Nditgesellschaft Auf Aktien | Enzyme granules containing phosphated starch |
US6350728B1 (en) | 1996-12-11 | 2002-02-26 | Henkel Kommanditgesellschaft Auf Aktien (Kgaa) | Coated enzyme preparation with an improved solubility |
US7803604B2 (en) | 2000-07-28 | 2010-09-28 | Henkel Ag & Co. Kgaa | Amylolytic enzyme extracted from Bacillus sp. A 7-7 (DSM 12368) and washing and cleaning agents containing this novel amylolytic enzyme |
US7888104B2 (en) | 2000-11-28 | 2011-02-15 | Henkel Ag & Co. Kgaa | Cyclodextrin glucanotransferase (CGTase), obtained from<I>Bacillus agaradherens<λ>(DSM 9948) and detergents and cleaning agents containing said novel cyclodextrin glucanotransferase |
US6979669B2 (en) | 2001-08-30 | 2005-12-27 | Henkel Kommanditgesellschaft Auf Aktien | Encapsulated active ingredient preparation for use in particulate detergents and cleaning agents |
US7300782B2 (en) | 2001-12-21 | 2007-11-27 | B.R.A.I.N. Biotechnology Research And Information Network Ag | Glycosyl hydrolases |
US7569226B2 (en) | 2001-12-22 | 2009-08-04 | Henkel Kommanditgesellschaft Auf Aktien (Henkel Kgaa) | Alkaline protease from Bacillus sp. (DSM 14392) and washing and cleaning products comprising said alkaline protease |
US7456143B2 (en) | 2002-12-20 | 2008-11-25 | Henkel Kommanditgesellschaft Auf Aktien (Henkel Kgaa) | Bleach-containing washing or cleaning agents containing a sulfate/silicate coated percarbonate |
CN117417922A (en) * | 2023-12-13 | 2024-01-19 | 北京科为博生物科技有限公司 | High-temperature-resistant alkaline protease and gene and application thereof |
CN117417922B (en) * | 2023-12-13 | 2024-03-15 | 北京科为博生物科技有限公司 | High-temperature-resistant alkaline protease and gene and application thereof |
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