GB1151608A - A Method for Determining Vitamin B12 - Google Patents
A Method for Determining Vitamin B12Info
- Publication number
- GB1151608A GB1151608A GB47604/67A GB4760467A GB1151608A GB 1151608 A GB1151608 A GB 1151608A GB 47604/67 A GB47604/67 A GB 47604/67A GB 4760467 A GB4760467 A GB 4760467A GB 1151608 A GB1151608 A GB 1151608A
- Authority
- GB
- United Kingdom
- Prior art keywords
- vitamin
- particles
- sample
- radioisotope
- oct
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/804—Radioisotope, e.g. radioimmunoassay
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
1,151,608. Estimation of vitamin B 12 . PHARMACIA AB. 19 Oct., 1967 [21 Oct., 1966], No. 47604/67. Heading B1X. [Also in Division C3] Vitamin B 12 in an aqueous sample is estimated by contacting particles of a water insoluble epichlorhydrin crosslinked dextran derivative which has covalently bound to it the substance "intrinsic factor" (a muco protein from the ventriculus mucous membrane, and which is capable of binding Vitamin B 12 ; see Division C3), with the sample and with a predetermined amount of vitamin B 12 labelled with a radioisotope, and subsequently separating the particles from the sample liquid and measuring the radioactivity o,f the particles and/or of the liquid. Suitably the radioisotope is <SP>58</SP>Co. In the example a 0.05M tris-buffer at pH 7.4 containing NaCl, bovine serum albumin, and sodium azide is used as aqueous medium. The dextran reagent may be supplied in ampoules together with ampoules containing the labelled vitamin B 12 , to make a test pack.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE14397/66A SE326845B (en) | 1966-10-21 | 1966-10-21 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1151608A true GB1151608A (en) | 1969-05-14 |
Family
ID=20299036
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB47604/67A Expired GB1151608A (en) | 1966-10-21 | 1967-10-19 | A Method for Determining Vitamin B12 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US3505019A (en) |
| AT (1) | AT286243B (en) |
| BE (1) | BE705412A (en) |
| CH (1) | CH476493A (en) |
| DE (1) | DE1673015B1 (en) |
| DK (1) | DK120509B (en) |
| ES (1) | ES346273A1 (en) |
| FI (1) | FI48509C (en) |
| GB (1) | GB1151608A (en) |
| NL (1) | NL159191B (en) |
| NO (1) | NO122457B (en) |
| SE (1) | SE326845B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3853987A (en) * | 1971-09-01 | 1974-12-10 | W Dreyer | Immunological reagent and radioimmuno assay |
| GB1458978A (en) * | 1973-12-11 | 1976-12-22 | Radiochemical Centre Ltd | Saturation analysis |
| US3936440A (en) * | 1974-05-22 | 1976-02-03 | Drexel University | Method of labeling complex metal chelates with radioactive metal isotopes |
| US4225784A (en) * | 1976-06-17 | 1980-09-30 | Smith Kline Instruments, Inc. | Covalently bound biological substances to plastic materials and use in radioassay |
| US5227311A (en) * | 1988-10-11 | 1993-07-13 | Abbott Laboratories | Intrinsic factor to determine B12 |
| DE3900650A1 (en) * | 1989-01-11 | 1990-07-12 | Boehringer Mannheim Gmbh | VITAMIN B12 DETERMINATION |
| US6942977B1 (en) | 1991-04-09 | 2005-09-13 | Bio-Rad Laboratories, Inc. | Immunoassays for determining vitamin b12, and reagents and kits therefor |
-
1966
- 1966-10-21 SE SE14397/66A patent/SE326845B/xx unknown
-
1967
- 1967-10-14 NO NO170132A patent/NO122457B/no unknown
- 1967-10-16 US US675323A patent/US3505019A/en not_active Expired - Lifetime
- 1967-10-18 DK DK517167AA patent/DK120509B/en unknown
- 1967-10-19 GB GB47604/67A patent/GB1151608A/en not_active Expired
- 1967-10-20 CH CH1470767A patent/CH476493A/en not_active IP Right Cessation
- 1967-10-20 NL NL6714282.A patent/NL159191B/en not_active IP Right Cessation
- 1967-10-20 FI FI672828A patent/FI48509C/en active
- 1967-10-20 DE DE19671673015 patent/DE1673015B1/en active Pending
- 1967-10-20 BE BE705412D patent/BE705412A/xx unknown
- 1967-10-20 AT AT950567A patent/AT286243B/en not_active IP Right Cessation
- 1967-10-20 ES ES346273A patent/ES346273A1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| NL6714282A (en) | 1968-04-22 |
| DE1673015B1 (en) | 1972-05-04 |
| SE326845B (en) | 1970-08-03 |
| DK120509B (en) | 1971-06-07 |
| AT286243B (en) | 1970-11-25 |
| FI48509B (en) | 1974-07-01 |
| NL159191B (en) | 1979-01-15 |
| BE705412A (en) | 1968-03-01 |
| FI48509C (en) | 1974-10-10 |
| CH476493A (en) | 1969-08-15 |
| NO122457B (en) | 1971-06-28 |
| ES346273A1 (en) | 1969-01-01 |
| US3505019A (en) | 1970-04-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PCNP | Patent ceased through non-payment of renewal fee |