FR2872041A1 - Agent for promoting cutaneous penetration of topically applied cosmetic or pharmaceutical active agents, comprising extract of cells of Ranunculaceae plant, especially lesser celandine - Google Patents

Abstract

The use of an extract (I) of the cells of plant(s) of the Ranunculaceae family is claimed in (or for the preparation of) a composition containing active agent(s) (A), as agent for: (i) promoting cutaneous penetration of (A), (ii) reducing the fixation of (A) to skin tissue proteins or (iii) saturating the metabolic pathway of (A) at the skin. An independent claim is included for cosmetic compositions comprising effective amounts of (A) and (I) in a suitable medium.

Description

DERMAL COMPOSITION

COMPRISING A FACTORY EXTRACT

  The present invention relates to the formulation of preparations for topical use, in particular to the use of penetration promoters of the active principles in creams for cosmetic use.

  It relates to the use of a cell extract derived from at least one plant belonging to the family Ranunculaceae as cutaneous penetration agent of an active ingredient. Another object of the present invention is a cosmetic composition comprising such a cell extract in combination with an active agent in a physiologically acceptable medium to promote the penetration of the active agent during its topical application.

  Percutaneous absorption is defined as the penetration of matter through the skin, without incision of it. The skin is composed of three large layers of layers: the epidermis, the dermis and the hypodermis, forming a protective envelope for the body, in particular by limiting the exchange of matter between the inside and the outside. Crossing of the cutaneous barrier by substances coming from the outside is done more or less easily according to a certain number of parameters, either by passing through the various cell layers of the skin, or by passing through the intercellular spaces.

  The formulation of preparations including an active ingredient for cosmetic or therapeutic purposes must therefore take into account the physicochemical characteristics of the skin to enable it to cross or not the skin barrier, which will confer a more or less rapid action, lasting and intense, in depth or surface. In the case where the active ingredient must produce its effect in the subcutaneous cell layers, penetration without metabolic transformation in the skin is sought.

  Many studies have been conducted to develop compositions penetrating more effectively through the skin. It is known that the nature of the skin, linked for example to age, as well as the method of application of the preparations can influence skin penetration. Of course, the nature of the ingredients also plays a role, whether it is the active ingredient, the excipient or any additive intended to promote the absorption of the active ingredient. However, if the active ingredient can participate in the skin penetration by its own physicochemical properties (viscosity, liposolubility, water solubility, concentration, molecular weight, ...), it is a mandatory element for a given use, that there can be no question of changing.

  It is known that the excipient also plays an essential role. It influences the cutaneous penetration by its more or less marked affinity properties with the constituent cells of the skin. It has no pharmaceutical effect, but it contains inactive substances that are intended to convey the active ingredient to its place of action.

  Ingredients known as absorption promoters may also be used. They are absorption accelerators whose function is to increase either the solubility or the diffusion of the active ingredients through the skin. The substances used to date belong to three main classes. A first class consists of anionic substances of the disodium laureth sulphosuccinate type, whose effectiveness is interesting but whose use is limited in cosmetics because they are unfortunately irritating. Another class includes glycol ethers, now banned in cosmetics, except ethoxydiglycol. This product, known under the trade name TranscutolTM (Gattefossé, France), is not devoid of toxicity and is still suspected by consumers. The third class consists of DMSO (Dimethylsulfoxide), banned for cosmetic use.

  So far, the substances or components used in the preparation of cosmetic products have not been entirely satisfactory vis-à-vis the transcutaneous penetration of said products.

  The problem posed is therefore to promote the cutaneous penetration of an active ingredient in order to increase and / or prolong its action, while minimizing the risks of occurrence of undesirable effects, in particular skin irritation or reactions. allergic. It has been found, quite unexpectedly, that the use of an extract of cells from at least one plant of the family Runcunculaceae had interesting properties to stimulate the penetration through the skin of active ingredients present in preparations for topical application.

  It is known that, at present, there is an increasing use of products of plant origin as active ingredients, whether in the cosmetic or pharmaceutical field. These products are indeed considered to be less allergenic and more generally to better respect the natural biological balances. However, to date, a plant extract has not been used as a skin penetration agent in a cosmetic composition.

  The present invention relates to the use in a composition or for the preparation of a composition comprising at least one active principle; an effective amount of a cell extract of at least one plant of the family Rennunculaceae as dermal penetration agent of said active principle. Penetration agent is an agent that promotes the penetration of an active ingredient through the skin barrier, so as to accelerate, increase or prolong the action of said active ingredient. In addition to the advantages described above, their use makes it possible to reduce the doses of administered assets, which also has an impact on the good tolerance of the products. The resulting reduction in production costs is an aspect to be taken into account as well.

  These cell extracts may preferably be used as agents making it possible to reduce the attachment of said active ingredient to the proteins of the skin tissues. More preferably, these cell extracts can be used as cutaneous penetration agents of an active agent, making it possible to saturate the metabolic pathway of said active ingredient in the skin.

  It has been demonstrated for the first time that the varieties of this plant family contain in their juices components which may act on the permeability of the skin. Their roots are known to contain biologically active triterpene saponosides. Extracts prepared from the roots are the active ingredient of drugs prescribed today for their action on the blood circulation. The genus Ranunculus ficaria also called Ficaire in particular, has been known for centuries for its anti-hemorrhoidal properties. It has now been discovered that a renunculaceae cell extract can be used in combination with an active agent in a physiologically acceptable medium to promote the penetration of the active agent during its topical application.

  The saponosides of Ficaire, heterosides of hederagenin and oleanolic acid, are particularly interesting because among the least irritating of the plant kingdom. Therefore, the cell extract according to the invention is preferably an extract of tubular cells of Ranunculus ficaria.

  The renunculaceae cellular extract acting on the cutaneous permeability is combined in a composition with an active agent intended to act on the skin by cutaneous application. Advantageously in the use according to the invention, said at least one active principle is chosen from slimming agents, soothing agents and healing agents.

  More precisely, the said at least one active ingredient is preferably chosen from among the purine bases, xanthine bases, flavones, isoflavones, caffeine, theophylline, theobromine. The use as a penetrating agent of a renunculaceae cell extract is indeed particularly effective with respect to these compounds. It has been hypothesized that the saponosides present in the renunculaceae cell extract saturate the protein sites involved in the metabolism of these compounds at the level of the skin. Indeed, said active compounds, such as the saponosides of the cell extract, are xanthic bases or related structures, the metabolism of which at the level of the skin is under the control of the microsomal oxidative system linked to the cytochromes responsible for the N-demethylations. being saturable.

  The subject of the present invention is also cosmetic compositions containing, in a physiologically acceptable medium, an effective amount of at least one active principle and an effective amount of a cell extract of at least one plant of the family Ranunculaceae. Preferably, the composition according to the invention contains a cellular extract of Ranunculus ficaria tubers.

  According to another advantageous characteristic of the claimed composition, said at least one active principle is chosen from slimming agents, soothing agents and healing agents. Preferably, said at least one active ingredient is selected from purine bases, xanthine bases, flavones, isoflavones, caffeine, theophylline, and theobromine. It can be provided in the composition according to the invention in pure form or by means of complex substances. For example, a chamomile extract, a vegetable source of flavones and flavonoids, can be used as a soothing agent; terpenes of Centella asiatica can be used as healing and antipruritic agents; caffeine is used as a lipolytic agent.

  The cell extract can be prepared according to one of the many methods known to those skilled in the art for extracting compounds from plant tissues. However, the vegetable extracts obtained by the conventional methods of alcoholic or hydro-alcoholic extraction, by maceration or lixivation, have the disadvantage of having only a fraction of the substances contained in the plant, these being either not extracted , or degraded during the extraction and concentration steps. These processes can in particular cause the dissociation of heat-sensitive molecular units and thus reduce their effectiveness.

  Particularly advantageously, the extract used in a composition according to the invention or for its preparation, is a total cellular juice obtained by cryoextraction, containing the cold-soluble constituents belonging to the juice of said plant, in particular 2872041 of sugars, salts minerals, proteins, etc ... According to this method, the plant material is crushed and screened in the frozen state so that the cellulosic membranes are broken. Then one or more pressing steps of the fractions obtained are carried out at a temperature of a few degrees above zero. Clarification and filtration are carried out after stabilization by addition of propylene glycol. This juice can be used as it is in the composition according to the invention. It can be stored for several months at a refrigerated temperature, protected from light. The cellular juice in question here is therefore a solution obtained by cold pressing the plant material called "total juice". This juice has the advantage of having all the active ingredients of the plant at a high concentration. It is free of cellulosic compounds and protoplasts removed by filtration and it contains most of the water-soluble substances of the plant's juice, in the active form in which they are found naturally in the plant.

  In particular, said total cellular juice contains saponosides stably bound to at least one sugar, this associated form naturally present in the renunculaceae cell juice, having revealed an increased activity compared to the dissociated form obtained by the extraction methods. conventional. On the contrary, in the extracts used to date for the preparation of cosmetic or dermal compositions, the base and its sugar are dissociated, mainly because of the thermal conditions of preparation of the extracts, while this association is preserved in the cell juice. Ficaire contains mainly hederagenin, and secondarily oleanolic acid. Figure 1 shows the formula of these glycosides in combination with sugars, which may be hederagenine (with R = CH2OH), or oleanolic acid (with R = CH3) (after Jean Bruneton, 1993, "Pharmacognosy , phytochomy, medicinal plants ", Ed. Lavoisier).

  According to a preferred embodiment, the composition according to the invention contains said cell extract in a proportion of 0.1% to 20%, preferably 1% to 10% by weight relative to the total weight of the composition.

  In an alternative embodiment, the composition according to the invention may contain, in addition to the ingredients described above, one or more other components whose action, complementary to that of the active ingredient, is chosen according to the application sought for the cosmetic composition considered. These additional components may be chosen in particular from draining agents, firming agents, anti-irritants, sunscreens, anti-inflammatory agents. The composition may for example incorporate a green tea extract for its draining properties, an extract of climbing ivy which facilitates the microcirculation and decreases the sensitivity to pinching of cellulite, a firming agent such as an oily extract of Kigelia Africana, an ant an irritant such as glycerrhetinic stearate, UV filters such as octyl-methoxycinnamic acid, octylsalicylic acid or butyl-methoxydibenzoylmethane, and also an anti-inflammatory such as a-bisabolol. These components are known to those skilled in the art who know how to use them at the dosage suitable for the intended use.

  To form the composition according to the invention, the active component (s) and the penetrating agent are introduced into a physiologically acceptable medium. This may consist of water and / or at least one organic solvent in proportions determined according to the rules of the art. Similarly, the composition contains one or more additives selected from the group consisting of gelling agents, thickeners, preservatives, emulsifiers, perfumes, moisturizing agents, pigmenting agents, emollients, surfactants.

  Among the gelling agents, mention may be made of xanthan gum, carboxyvinyl polymers such as Ultrez 1OTM (BF Goodrich USA) or their triethanolamine salts such as Carbomer-TEATM (BF Goodrich USA). Examples of thickeners are cetyl alcohol, cetyl palmitate or cetostearyl alcohols, such as Stenol C7 (Cognis, Germany) or Sipol C16, C18, C7 (Cognis, Germany). A preservative may be phenoxyethanol, methyl paraben, propyl paraben, trisodium EDTA, sodium benzoate. An emulsifier is chosen for example from glycerol stearate, sucrose sesquistearate or the many commercial preparations available, for example Polysorbate 60 (Quimasso, USA), Arlacel 60 (Quimasso, USA), Arlacel 165 (Quimasso, USA), Tween 60 (Quimasso, USA), Span 60 (Quimasso, USA), PEG Methylglucose sesquistearate (Amerchol, USA), PEG 20 Oleyl Ether (Croda, UK). Also, an emollient may be included, such as capric and caprylic triglycerides, methyl polysiloxane, octyl dodecanol, isononyl isononanoate. Finally, if desired, a pigment, an antioxidant, a moisturizing agent, a surfactant or a solvent for the sunscreens, for example a C12-C15 alkyl benzoate, may be added, according to the known rules of the art.

  The composition may also comprise other ingredients commonly used for the formulation of cosmetic products, for example a vehicle such as liquid petrolatum, or oleic sunflower oil, a humectant such as glycerine or butylene glycol, a neutralizer such as triethanolamine, a complexing agent such as disodium and trisodium EDTA or a consistency agent such as glycol stearate.

  The amounts of these various additives and adjuvants are those conventionally used. Examples will illustrate this aspect of the practice of the invention utilizing established practices in the technical field of formulating products for topical application.

  The compositions thus obtained have the appearance of a white or colored cream, an ointment, a milk, a lotion or a paste, depending on the formulation chosen for the intended use.

  Another object of the present invention is the use of a cell extract derived from a plant of the family Rennunculaceae in a composition or for the preparation of a composition as described above. According to a preferred embodiment, the use of a total cellular juice derived from Ranunculus ficaria in a composition or for the preparation of a composition according to the invention is claimed.

  The examples which follow are intended to illustrate the invention, without presenting any limiting character.

  EXAMPLE 1 - Characteristics of the plant extract A cellular juice prepared from fresh roots of Ficaire is preserved in a propylene glycol / water mixture without preservative. This product, prepared according to the teachings of the patent application FR 89 14186, and marketed by Greentech (France), has the following characteristics: E Organoleptic characteristics Clear appearance Color coppery yellow-brown Characteristic odor E Physico-chemical characteristics Solubility: 1% in soluble water Solubility: 1% in 95 soluble alcohol Dry matter (1 g at 100-105 C for 16-20 hours): 0.5% 0.02% Density at 25 C: 1.02 pH (direct ): 6.5 0.5 Refractive index at 25 C: 1.375 - 1.395 2872041 8 E Microbiological characteristics Total aerobic germs <100 cfu / ml Yeasts and molds <100 cfu / ml Enterobacteria <1 cfu / ml EXAMPLE 2 - Implementation evidence of the effect on the skin penetration 1) - Principle To prove the role of penetrating agent of a cellular extract of Ficaire, the capacity of this one to convey through the skin an asset used as slimming was studied . To do this, the kinetics of the transcutaneous passage of caffeine contained in a preparation in the presence or absence of a Ficaire extract were compared. The skin permeability tests were carried out ex-vivo on human skin by the technique described by T. J. FRANZ (J. Invest Dermatol., 1975, 64: 190-195).

  2) - Composition of Creams Test and Control All contents are expressed in grams per 100 grams of composition. The cell extract of Ficaire used is as described in Example 1.

  COMPONENTS Test (% by weight) // Control (% by weight) Ficaire Medulat (Greentech, France) 5,000 // demineralized water 5,000 Caffeine 1,000 1,000 Glycerin 5,000 5,000 2-phenoxyethanol 0,700 0,700 Ultrez 10 0,300 0,300 Polysorbate 60 1,900 1, 900 Arlacel 60 1,100 1,100 Capriliq. Capriq. Triglyceride 5,000 5,000 Stenol C7 / Sipol Cl6C18C7 1,500 1,500 Vaseline oil A 50 / Mark 3,000 3,000 Triethanolamine 0,300 0,300 Filtered demineralised water 75,200 75, 200 2872041 9 The preparation of the control cream is carried out as follows: In a suitable container, the caffeine, glycerine and 2-phenoxyethanol are mixed in water and brought to 70 C. Then the Ultrez 10 is dispersed until a homogeneous paste. In parallel, polysorbate 60, Arlacel 60, caprylic caprylic triglyceride, Stenol C7 / Sipol Cl 6, C18, C7 and petrolatum oil are mixed at 72 ° C. The contents of the first vessel are incorporated into this mixture and stirred for 10 minutes before neutralizing the pulp with triethanolamine. When the dough is cooled to 25 ° C., 100% is completed with the remaining filtered water.

  The cream Test is prepared according to the same protocol, but when the paste reaches the temperature of 35 C, the cell extract of Ficaire is added, then complete with 100% of filtered water.

  3) - Experimental protocol A skin fragment (explant of human skin) freshly collected is placed in a cell of Franz. The transfer surface is about 1 cm2. The receiving medium with a volume of about 1 ml consists of a solution of culture medium. The experimental device is shown in Figure 2.

  The preparation is maintained at 37 ° C. in a 5% CO2 incubator. After application of the cream to the surface of the skin, samples of the receiving medium are made at time intervals T + 1 hour, T + 2 hours, T + 4 hours, T + 8 hours, and T + 24 hours. Caffeine is assayed by HPLC in the receiving medium samples taken. This procedure is carried out under the same conditions, with the caffeine cream containing the extract of Ficaire (Cream Test) and that without extract of Ficaire (Control cream).

  4) - Determination of Caffeine in the Receiving Medium The caffeine assay in the receiving medium was carried out by HPLC (High Performance Liquid Chromatography), with detection by UV absorption at 280 nm. The analysis conditions are as follows: É C18 grafted reverse phase column; É Direct injection of the sample after filtration (0.22 m); Flow: 1 ml / min; Solvent A: water / trifluoroacetic acid (TFA) 1% (97.5 / 2.5, v / v) Solvent B: acetonitrile (ACN) / solvent A (80/20; v / v); É Elution gradient: see table 1 30 35 2872041 10

TABLE 1

  time (min)% B time (min)% B 0.0 18 33.0 80 11.5 18 36.0 100 18.5 23 42.0 100 22.5 24.5 43.0 18 28.5 31 , 48.0 18 31.5 40 5) - Results The obtained assay values are read with respect to a standard curve, established from pure caffeine (Sigma) and relating the injected quantity of caffeine with the absorbance at 280 nm. The standard curve is the equation y = 1464.396 x + 13.756, with r2 = 1.000.

  The results obtained for the cream with extract of Ficaire (Test) and without extract of Ficaire (Témoin) are the following (Table 2). They are represented by the curve Figure 3.

TABLE 2

  Caffeine concentration (g / 100 l) Time Cream Control Cream Test% Test / Control 1h 0.53 0 2 h 0.40 0.34 4 h 0.48 0.95 8 h 2.48 2.97 24 h 9 Conclusions In the experimental conditions described, the results show a progressive increase in the transcutaneous passage of caffeine as a function of time. This transcutaneous passage is potentiated in the Test formulation compared to the Control formulation after 24 hours of contact.

  EXAMPLE 3 - Formulation of an anti-irritant cream An anti-irritant cream comprising 5% of Ficaire cell extract as described in Example 1 and a soothing agent (Chamomile extract containing flavones and flavonoids), as well as a anti-inflammatory (a-bisabolol) is prepared using the following ingredients: COMPONENTS QUANTITY (% wt.) Ficaire cell extract 5,000 Chamomile extract 1,000 abisabolol 0,500 Butylene glycol 1-3 5,000 Phenoxyethanol 0,500 Methyl paraben 0,150 Disodium EDTA 0,100 Triglyceride Caprylic caprylic 8,000 Cetyl alcohol 1,000 Glycerol stearate 3,500 Tween 60 3,500 Span 60 2,500 Methyl polysiloxane V 100 1,000 Perfume 0,500 Demineralized water QSP 100 Procedure: In a suitable container, butylene glycol 1-3, phenoxyethanol, methyl paraben and disodium EDTA, at a temperature of 75 ° C. In a second container, capric triglyceride is introduced with approximately 95% of the water. caprylic, chamomile extract, a-bisabolol, cetyl alcohol, glycerol stearate, Tween 60, Span 60 and methyl polysiloxane. When the mixture is homogeneous and the temperature reaches 77 ° C., the contents of the two containers are combined and stirred for 10 minutes and then allowed to cool. When the paste is at 30 ° C., the Ficaire cellular extract and the perfume are added, and the mixture is mixed for 5 minutes, then adjusted with the remaining water and allowed to cool to 25 ° C. before conditioning.

  EXAMPLE 4 Formulation of a Cream for the Oval of the Face The cell extract of Ficaire used is that described in Example 1.

  COMPONENTS QUANTITY (w / w%) Cellular extract of Ficaire 3,000 Caffeine 5,000 Green tea extract 2,000 Octyl dodecanol 1,500 Isononyl isononanoate 2,000 Cetyl palmitate 1,500 Glycol stearate 2,500 Methylglucose sesquistearate 2,500 PEG20 methylglucose sesquistearate 1,500 Glycerinetic stearate 0.100 Propyl paraben 0.150 Phenoxyethanol 0.500 Trisodium EDTA 0.100 Butylene glycol 5,000 Fragrance 0.200 Demineralized water QSP 100 Method of preparation: The cream is prepared in two phases. Phase A comprising octyl dodecanol, isononyl isononanoate, cetyl palmitate, glycol stearate, methylglucose sesquistearate, PEG methylglucose sesquistearate, glycerhetinic stearate, propyl paraben, is heated to 60 C. The aqueous phase B comprising 95% of water, preservatives and caffeine, is heated to 65 C and then poured into phase A. The whole is stirred for 30 minutes. Then allowed to cool gradually, and added at 40 C, the green tea extract; at 2872041 13 35 C, the cell extract of Ficaire and at 30 C, the perfume. It is completed with the remaining water and after homogenization, it is transferred to the desired packaging.

  EXAMPLE 5 Formulation of a Slimming Milk The cell extract of Ficaire used is as described in Example 1.

  COMPONENTS QUANTITY (% weight) Ficaire cell extract 5,000 Caffeine 2,000 Climbing ivy extract 2,000 Arlacel 165 8,000 PEG 20 oleyl ether 2,000 Cetyl alcohol 1,200 Oleic sunflower oil 4,000 Kigelia Africana oily extract 5,000 Isononyl isononanoate 2,000 Propyl paraben 0,100 Xanthan gum 0,400 Methyl paraben 0.200 Phenoxyethanol 0.500 Sodium benzoate 0.500 Trisodium EDTA 0.100 Butylene glycol 5.000 Perfume 0.500 Demineralized water QSP 100 The preparation of the slimming agent is carried out as follows.

  A phase A comprising Arlacel 165, PEG oleyl ether, cetyl alcohol, oleic sunflower oil, isononyl isononanoate and propyl paraben is heated to 78 ° C. Kigelia extract is added to last moment. A phase B comprising approximately 95% of the water, methyl paraben, phenoxyethanol, sodium benzoate, trisodium EDTA, butylene glycol and caffeine, is brought to 80 C. At the last moment, the xanthan gum is dispersed in this paste. Phase B is then poured into phase A and the mixture is stirred for 15 minutes. It is allowed to cool slowly and the ivy extract is added at 40 ° C., the Ficaire cell extract at 35 ° C. and finally the perfume at 30 ° C. The milk thus obtained is left to stand for 48 hours before conditioning.

  EXAMPLE 6 Formulation of an anti-irritant sunscreen The cellulose extract used is as described in Example 1.

  COMPONENTS QUANTITY (% wt.) Ficaire cell extract 5,000 Octyl methoxycinnamate 7,000 Octyl salicylate 3,500 Butyl methoxydibenzoylmethane 4,000 Centella asiatica terpenes 0.100 Green tea extract 2,000 a-bisabolol 0.200 Arlacel 165 5,000 Tween 60 2,500 Span 60 3,000 Isononyl isononanoate 5,000 Alkyl benzoate C12-C15 10,000 Cetyl alcohol 1,500 Methyl paraben 0,100 Propyl paraben 0,050 Phenoxyethanol 0,500 Trisodium EDTA 0,100 Butylene glycol 5,000 Carbomer - TEA 0,200 Xanthan gum 0,400 Perfume 0,300 Demineralized water QSP 100 The method of preparation is as follows In a suitable container, the Arlacel 165, Tween 60, Span 60, isononyl 2872041 isononanoate, C12-C15 alkyl benzoate and cetyl alcohol and heated to 78 C. Then one half of methyl paraben, propyl paraben and a-bisabolol. It is mixed until complete dissolution and octyl methoxycinnamate, octyl salicylate and butyl methoxydibenzoylmethane are added at the last moment. In another container, 95% of the water is introduced and the remainder of the methyl paraben, phenoxyethanol, trisodium EDTA and butylene glycol, which is heated to 80 C. The carbomer-TEA is then added, the mixture is mixed until a gel is formed and the xanthan gum is added. Stirring is continued until complete homogenization. Then, the contents of the first container is poured on the gel. The whole is mixed and stirred for 15 minutes. During cooling, the terpenes of Centella asiatica are incorporated at 50 ° C.; at 35 ° C. the cell extract of Ficaire, and at 30 ° C. the perfume. Complete with the remaining filtered water and allow to stand for 48 hours before conditioning.

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Claims (15)

16 CLAIMS   1- Use in a composition or for the preparation of a composition comprising at least one active principle, an effective amount of a cell extract of at least one plant of the family Ranunculaceae, as skin penetration agent of said principle active.   2- Use in a composition or for the preparation of a composition comprising at least one active principle of an effective amount of a cell extract of at least one plant of the family Rennunculaceae, as agent for reducing the fixation of said active ingredient to the proteins of the skin tissues.   3- Use in a composition or for the preparation of a composition comprising at least one active principle, of an effective amount of a cell extract of at least one plant of the family Ranunculaceae, to saturate the metabolic pathway of said principle active in the skin.   4- Use according to one of claims 1 to 3, characterized in that said cell extract is an extract of tubercle cells of Ranunculus ficaria.   5- Use according to one of claims 1 to 4, characterized in that said at least one active ingredient is selected from slimming agents, soothing agents, healing agents.   6- Use according to one of claims 1 to 5, characterized in that said at least one active ingredient is selected from purine bases, xanthine bases, flavones, isoflavones, caffeine, theophylline, theobromine.   7- Cosmetic composition characterized in that it contains, in a physiologically acceptable medium, at least - an effective amount of at least one active ingredient; and an effective amount of a cell extract derived from at least one plant of the family Rennunculaceae.   8. Composition according to claim 7, characterized in that the cell extract is an extract of tubers of Ranunculus ficaria.   9- Composition according to one of claims 7 or 8, characterized in that said at least one active ingredient is selected from slimming agents, soothing agents, and healing agents.   10- Composition according to one of claims 7 to 9, characterized in that said at least one active principle is selected from purine bases, xanthine bases, flavones, isoflavones, caffeine, theophylline, and theobromine.   11. Composition according to one of claims 7 to 10, characterized in that the cell extract is the total cellular juice obtained by cryoextraction, containing the cold-soluble constituents belonging to the juice of said plant.   12. Composition according to the preceding claim, characterized in that said total cellular juice contains saponosides stably bound to at least one sugar.   13- Composition according to one of claims 7 to 12, characterized in that it contains said cell extract in a proportion of 0.1% to 20%, preferably 1% to 10%, by weight relative to the total weight of the composition.   14 - Composition according to one of claims 7 to 13, characterized in that it further contains one or more other components selected from draining agents, firming agents, anti-irritants, sunscreens, anti-inflammatories.   15- Composition according to one of claims 7 to 14 characterized in that the physiologically acceptable medium consists of water and / or at least one organic solvent.   16- Composition according to one of claims 7 to 15 characterized in that it further contains at least one additive selected from the group consisting of gelling agents, thickeners, preservatives, emulsifiers, perfumes, pigmenting agents, emollients, surfactants.   17- Composition according to one of claims 7 to 16 characterized in that it has the appearance of a white or colored cream, an ointment, a milk, a lotion or a paste.   18- Use of a cell extract derived from a plant of the family Rennunculaceae in a composition or for the preparation of a composition according to any one of claims 7 to 17.   19- Use of a total cellular juice derived from Ranunculus ficaria in a composition or for the preparation of a composition according to any one of claims 10 to 17. 15 20 25 30