FR2811671A1 - BIODEGRATION-RESISTANT POLYMER (S) HYDROGEL, PREPARATION AND USE AS TISSUE REGENERATION SUPPORT - Google Patents

Abstract

The invention concerns a hydrogel of at least a polymer selected among proteins, polysaccharides and derivatives thereof. The invention is characterised in that the sterile hydrogel contains said crosslinked polymer as well as an efficient amount, for its subsequent use, in regions of the human or animal body rich in free radicals, of at least an antiseptic, amount efficient for protecting it against the free radicals. The invention also concerns the preparation of said hydrogel and its use as tissue regeneration support.

Description

The subject of the present invention is: - a hydrogel of at least one polymer

  chosen from proteins, polysaccharides and their derivatives, particularly resistant to biodegradation, once implanted in the human or animal body; - a process for preparing said hydrogel; said hydrogel, for its use as a tissue regeneration support; - a tissue regeneration support based on said hydrogel; - The use of said hydrogel for the preparation of such a support

tissue regeneration.

  According to the invention, a new product is proposed which is particularly effective in its use as a tissue regeneration support, and more

  particularly as a filling gel for periodontal pockets.

  In the publication MIN. STOM., Vol. 17, 1968, pages 140-156, entitled "Acido ialurinico e periodontopatie", it is described injections of sodium hyaluronate, not crosslinked, not containing antiseptic, to treat

  three types of conditions linked to periodontitis. The anti-role is highlighted

  inflammatory of said hyaluronate per se and its ability to accelerate fibrillo-plastic differentiation. This document neither describes nor suggests the role of tissue regeneration support developed by sodium hyaluronate, conditioned by

original way according to the invention.

  Patent application EP-A-444 492 describes compositions for topical use which contain, as active principle, sodium hyaluronate, of high molecular weight. Said hyaluronate occurs, uncrosslinked, in an excipient, at low contents, generally between 0.005 and 10% by weight. Said compositions are intended for the therapy and prophylaxis of inflammatory affections of the oral cavity, for the hygiene of said oral cavity and for

cosmetic treatments.

  Patent application JP-A-1 5744 describes aqueous solutions, for external use, which contain, as active principle, hyaluronic acid or one of its salts (non-crosslinked), generally at the rate of 0.001 at

2% by weight.

  Said non-sterile aqueous solutions contain an effective amount of at least one antiseptic chosen from - benzetonium chloride, - chlorhexidine hydrochloride, - chlorhexidine gluconate, and - alkyldiaminoethylene glycine hydrochloride; said antiseptic playing the role of preservative within said solutions, protecting said solutions from bacteria capable of developing in their

  breast, during their storage, their conservation ...

  The four antiseptics listed above were selected in the

  since they do not generate a deposit within said solutions.

  The effective amount of preservative within the meaning of said document of the prior art (effective amount for preserving the product before use) has nothing to do with the effective amount of antiseptic within the meaning of the invention (effective amount of antiseptic for preserve said product (sterile before use) on

  its site of use: see below).

  In such a context, the Applicant wished to develop a tissue regeneration support, capable of intervening, in a persistent manner, in cavities of the human or animal body, such as periodontal pockets or on open superficial wounds, such as gingivitis and bedsores. . To this end, it has developed an original hydrogel. It actually offers original packaging

  for a type of hydrogel known per se.

  Thus, the subject of the present invention is firstly a hydrogel of at least one polymer chosen from proteins, polysaccharides and their derivatives. This type of hydrogel is obviously known per se. According to the invention, said hydrogel is original - in that it is sterile; and in that it contains said crosslinked polymer, as well as an effective amount, with reference to its subsequent use in areas of the human or animal body rich in free radicals, of at least one antiseptic, effective amount

  to protect said hydrogel from said free radicals.

  * The polymer in question (the polymers in question) is (are) chosen from proteins, polysaccharides and their derivatives (and mixtures). It (s) is (are) especially chosen (s) from: -the following proteins: collagen, albumin, elastin; collagen being very particularly preferred; and - the following polysaccharides and polysaccharide derivatives: hyaluronic acid and its salts, chondroitin sulfates, keratane sulfates, heparin, alginic acid, starch, carboxymethylcellulose, hydroxypropylmethylcellulose, chitosan; hyaluronic acid and its salts being very particularly preferred;

(- their mixtures).

  * Said polymer intervenes in the sterile hydrogel state. Said hydrogel is unlikely to be obtained sterile at the end of its preparation process (this hypothesis, however, not being entirely excluded). It generally undergoes, at the end of said preparation process, a sterilization treatment, advantageously in accordance with standard EN 556. Such a sterilization treatment generally consists of a heat treatment of the autoclave cycle type. After such sterilization treatment, said hydrogel was

  cleared of bacteria and other microbes it was likely to contain.

  The antiseptic for which it is responsible therefore does not intervene, in any way, with reference to said bacteria and other microbes; in no way, as a preservative of said hydrogel ... but with reference only to the future use of said hydrogel, to its intervention, as long as possible in areas of the

human or animal body.

  - The polymer of the type specified above intervenes in the crosslinked hydrogel. It is thus better able to control the viscosity of said hydrogel, with particular reference to its possible injection. But above all, due to its crosslinking, said polymer is made more resistant to heat (this point is not negligible insofar as it has been seen that the hydrogel is generally sterilized in an autoclave) and to biodegradation . It is more particularly thus made more resistant to enzymes capable of degrading it, after its implantation in the human or animal body. Its depolymerization speed is slowed down. Its duration of

  life within the human or animal body is thus prolonged.

  The crosslinking to be used on said polymer is within the reach of those skilled in the art. It is in any event adapted to the nature of said polymer and advantageously carried out at an optimized rate. The crosslinking rate must be sufficient with reference to the expected result, in particular of resistance to heat and to biodegradation by enzymes; it must remain reasonable with reference to the mode of use of said hydrogel. Thus, said hydrogel must, in certain uses, be capable of adapting to the shape of a cavity to be filled, be capable of being injected ... In other uses, it

  only intervenes on the surface, it may appear to be crosslinked at higher rates.

  * The hydrogel of the invention, sterile, based on a crosslinked polymer as specified above, for single use, also contains an effective amount

at least one antiseptic.

  This effective amount is defined, as already indicated, with reference to the subsequent use of the hydrogel, in areas of the human or animal body which are inflamed and therefore rich in free radicals. This effective amount is primarily intended to stop, limit the development of bacteria responsible for inflammation within the hydrogel and therefore limit the amount of free radicals. This effective amount is above all intended to protect said hydrogel, to prolong its lifespan (its existence), during its intervention in areas of the human or animal body as specified above: inflamed, rich in

free radicals.

  The hydrogel of the invention, as described above, is therefore, with reference to its use in the human or animal body, in particular as a tissue regeneration support, doubly protected from biodegradation. It is protected by the crosslinking of its constituent polymer and by the presence, in

  adequate amount of at least one antiseptic within it.

  Such a hydrogel is therefore likely to intervene persistently, to develop a beneficial action in the long term. This beneficial action is that of the intervening polymer: an action of tissue regeneration, of cell restructuring. According to the invention, it is therefore proposed an original packaging, very interesting, to the polymer in question; a

  conditioning which allows it to develop its beneficial action over the long term.

  The antiseptic (s) involved (s) is (are) advantageously a

  (of) compound (s) soluble in water.

  In the context of a particularly advantageous variant, it is the

chlorhexidine digluconate.

  The effective amount, referenced above, for the preferred antiseptic

  identified above, is generally 0.08 to 0.25% by mass (of the hydrogel).

  Advantageously, it is between 0.10 and 0.15% by mass. On reading these figures, it is confirmed that, according to the invention, said effective quantity is defined with reference to the use of the hydrogel and not with reference to its "simple" conservation. Said effective amount, within the meaning of the invention, is to be determined for each of the antiseptics in question. Its determination is within the reach of those skilled in the art. The hydrogel of the invention loaded with antiseptic (s) is moreover capable of containing other substances, other substances whose intervention

  is advantageous in the areas of the human or animal body concerned.

  Thus, said hydrogel advantageously contains deoxyribonucleic acid (DNA). This product is known to decrease the reaction

  inflammatory and promote tissue regeneration.

  The hydrogel of the invention is advantageously based on a polymer chosen from hyaluronic acid (Ha), its salts and their mixtures. Said polymer

  preferably consists of sodium hyaluronate (NaHa).

  Said hyaluronic acid (or one of its salts) can be obtained by extraction from animal tissues, rooster crests and umbilical cords, in particular ... It is advantageously obtained by bacterial route, by cellular route (therefore free any virus or prion contaminant). It is recommended, in fact, very particularly, for the preparation of a hydrogel of the invention, the intervention of sodium hyaluronate fibers, obtained by the bacterial route. This polysaccharide is particularly preferred for the preparation of a hydrogel of the invention suitable as a filling gel, persistent, periodontal pockets. The alveolo-dental ligament, rich in glycosaminoglycans and proteoglycans, is in fact particularly "greedy" for sodium hyaluronate. This compound, integrating perfectly into the structure, staying there

  long, constitutes an ideal cell reconstruction matrix.

  By way of illustration, with reference to said hyaluronic acid and its salts, it is possible to specify suitable levels of crosslinking, within the meaning of the invention. The polymer chosen from hyaluronic acid, its salts and mixtures of salts is thus advantageously crosslinked, via its hydroxy functions, by means of a crosslinking agent, at a crosslinking rate defined by the ratio: R = Total number of reactive functions of said crosslinking agent Total number of disaccharide units of hyaluronic acid molecules

between 0.15 and 0.45.

  At higher values of R, one obtains almost a solid, which makes

  an integral part of the present invention, but the use of which is more limited.

  We have seen that such a solid is no longer injectable, no longer suitable for filling a cavity ... As a crosslinking agent, any agent known to crosslink hyaluronic acid can be used via its hydroxy functions - cross-linking agent at least bifunctional - and in particular a polyepoxide or its derivatives. As such crosslinking agent, it is possible in particular to involve

  epichlorohydrin, divinylsulfone, 1,4-bis (2,3-epoxypropoxy) butane (or 1,4-

  bis (glycidyloxy) butane or 1,4-butanedioldiglycidylether = BDDE), 1,2-

  bis (2,3-epoxypropoxy) ethylene, 1- (2,3-epoxypropyl) -2,3époxycyclohexane ...

  It is not excluded from the scope of the invention to involve several

  crosslinking agent ... It is particularly recommended to use 1,4-

butanedioldiglycidylether (BDDE).

  The person skilled in the art knows, in any event, to control crosslinking

hyaluronic acid.

  In the context of the very particularly preferred variant, according to which the hydrogel of the invention is a hydrogel based on sodium hyaluronate, said sodium hyaluronate intervenes advantageously at a concentration of between 10 to 30 mg / g, particularly advantageous to a

  concentration between 18 and 22 mg / g.

  Those skilled in the art will in any event be able to play on the two parameters: polymer concentration / crosslinking rate of said polymer, in order to obtain a hydrogel of the invention at its convenience in terms of flexibility, injectability ... details have been given above, in no way limiting, with reference to hyaluronic acid. Those skilled in the art can easily understand that the hydrogels of the invention are available in the same way based on other polysaccharides, proteins or their mixtures. In general, said hydrogels can be obtained by the process described below which constitutes

  the second object of the present invention.

  Said method typically comprises the following successive steps: - the crosslinking of a polymer or a mixture of polymers, chosen from proteins, polysaccharides and their derivatives, - the purification of said (said) ) crosslinked polymer (s), - the addition to the latter (these) of an adequate amount (effective within the meaning of the invention) of at least one antiseptic, - sterilization, if necessary of said crosslinked polymer (s)

  loaded with said antiseptic (s).

  The crosslinking, purification and sterilization steps are steps per se known to those skilled in the art. Said sterilization step is obviously necessary if the upstream steps have not been implemented in

sterile conditions.

  However, it is emphasized that they have never been used, according to the prior art, with the intervention of at least one antiseptic intended for

  protect the hydrogel during its use, single use.

  According to its last aspect, the invention relates to the use of said hydrogel as a tissue regeneration support, the use of said hydrogel for the preparation of a tissue regeneration support, a tissue regeneration support based on said hydrogel, a method regeneration

  tissue using said hydrogel.

  The hydrogel of the invention, as described above, as obtained by the method described above, is perfectly suitable as a tissue regeneration support. It thus intervenes advantageously in the healing processes of open superficial wounds (bedsores, gingivitis ...), in the processes of

  filling of cavities (deep wounds, periodontal pockets ...).

  The main function of the hydrogel of the invention is not to release an active principle (the intervening polymer does not intervene, in any event, as active principle) but, by its persistent presence , durable, it is capable of permanently filling empty spaces in the human body, promoting the synthesis and proliferation of reconstruction cells (fibroblasts)

  in said empty spaces as well as on the surface.

  In the context of said empty spaces, such as periodontal pockets, cell synthesis and proliferation are not observed if said spaces remain vacuolated. On the other hand, if the filling of these spaces is long enough, cell restructuring can take place. In this spirit, the product of the invention is sufficiently protected from biodegradation to constitute an agent for

persistent filling.

  Who can do more, can do less. Thus, the hydrogel of the invention, which is particularly effective as a gel for persistent filling of cavities, such as periodontal pockets, is also effective in wound contexts.

  superficial openings such as bedsores, gingivitis ...

  The invention therefore also relates to: - a tissue regeneration support, in particular intended to intervene in open cavities or surface wounds of the human or animal body (see above), based on the hydrogel of the invention described more high; - the use of a hydrogel of the invention for the preparation of a tissue regeneration support, in particular intended to intervene (in a lasting, persistent way) in cavities (to be filled) and on open superficial wounds (to heal ); the use of said hydrogel for the preparation of a filling gel, in particular of periodontal pockets and the use of said hydrogel for the preparation of a covering gel, in particular of gingivites and bedsores; - methods of treatment of the human or animal body, during which tissue regeneration is aimed at. The hydrogel of the invention intervenes, in a lasting manner, in critical places, within cavities or on the surface (see more

  top), as a filling agent or as a collection agent.

  Said hydrogel intervenes effectively with reference to said tissue regeneration insofar as it has been doubly protected (by its

  crosslinking and by the presence of the antiseptic agent within it).

  The invention is now illustrated by the example below.

  Preparation and conditioning of the hydrogel * Sodium hyaluronate fibers (NaHa, of molecular mass Mw 2.106 Da), of bacterial origin, are used as raw materials,

  of polymer within the meaning of the invention.

  They are swelled in an aqueous 0.9% sodium hydroxide solution

  en masse. A gel at 14-15% by mass is then obtained.

  * 0.22 g of 1,4-butanedioldiglycidylether (BDDE, crosslinker) are dispersed in this gel, homogeneously. The mixture is then placed in the oven,

for 2 h 30, at 48 + 2 C.

  The resulting gel is swollen in phosphate buffers (pH 7, then pH 7.3); this in order to stabilize its pH and obtain a final concentration

22 to 24 mg NaHa / g (gel).

  * This gel is then purified by immersion in successive phosphate buffer baths in which it is freed of both the agent

  cross-linking agent (BDDE) and unreacted polymer (NaHa).

  The purified crosslinked gel obtained is characterized by a ratio R: R = Total number of reactive functions of the intervening crosslinking agent Total number of disaccharide units of the polymer molecules present

= 0.32.

  * 0.78 g of aqueous solution (20% by weight) of digluconate

  chlorhexidine are added to said crosslinked gel.

  The mixture is mechanically homogenized.

  Said homogenized mixture is then packaged in syringes which

are autoclaved.

  The hydrogel obtained, thus conditioned, is injectable with or without

appropriate cannula.

  Said hydrogel is a hydrogel within the meaning of the invention, sterile, which contains sodium hyaluronate, crosslinked, as well as an effective amount

  (0.12% by mass) of chlorhexidine digluconate.

  Use of said hydrogel The periodontal pocket of a patient is previously curetted, descaled and disinfected by a dentist. In the pocket thus prepared, said dentist injects the hydrogel as obtained above. When this hydrogel is biodegraded, a new injection is made ... and so on. In fact, the injected hydrogel takes longer and longer and we observe in the ex-parondale pocket a

tissue reorganization.

Claims (11)

  1. Hydrogel of at least one polymer chosen from proteins, polysaccharides and their derivatives, characterized in that said sterile hydrogel contains said crosslinked polymer, as well as an effective amount, with reference to its subsequent use in areas of the human or animal body rich in radicals   free, of at least one antiseptic, amount effective to ensure its protection against with respect to said free radicals.   2. Hydrogel according to claim 1, characterized in that said   i0 antiseptic consists of chlorhexidine digluconate.   3. Hydrogel according to claim 2, characterized in that said effective amount of said antiseptic is between 0.08 and 0.25% by mass,   advantageously between 0.10 and 0.15% by mass.   4. Hydrogel according to any one of claims 1 to 3,   characterized in that it also contains deoxyribonucleic acid (DNA).   5. Hydrogel according to any one of claims 1 to 4,   characterized in that said polymer is chosen from hyaluronic acid, its salts and their mixtures; in that said polymer advantageously consists of sodium hyaluronate.   6. Hydrogel according to claim 5, characterized in that it has been crosslinked, via its hydroxy functions, by means of a crosslinking agent, at a crosslinking rate defined by the ratio: R = Total number of reactive functions of said agent crosslinker Total number of disaccharide units of hyaluronic acid molecules between 0.15 and 0.45.   7. Process for the preparation of a hydrogel according to any one   of the preceding claims, characterized in that it successively comprises:   - crosslinking of said polymer (s); - Purification of said cross-linked polymer (s); - Adding thereto (these) an adequate amount of at least one antiseptic; - sterilization, if necessary, of said cross-linked polymer (s)   loaded with said antiseptic (s).   8. Hydrogel according to any one of claims 1 to 6 and / or   obtained according to claim 7, for its use as a support for tissue regeneration, in particular intended to intervene in cavities or on open superficial wounds.   9. Hydrogel according to any one of claims 1 to 6 and / or   obtained according to claim 7, for its use as a filling gel, in particular of periodontal pockets.   10. Hydrogel according to any one of claims 1 to 6 and / or   obtained according to claim 7, for its use as a covering gel,   including gingivitis and bedsores.   11. Tissue regeneration support, in particular intended to intervene 1o in cavities or on open superficial wounds, characterized in that it   comprises a hydrogel according to any one of claims 1 to 6 and / or obtained according to claim 7.