FI20096288A0 - Formulations and Methods for Culturing Stem Cells - Google Patents

Formulations and Methods for Culturing Stem Cells

Info

Publication number
FI20096288A0
FI20096288A0 FI20096288A FI20096288A FI20096288A0 FI 20096288 A0 FI20096288 A0 FI 20096288A0 FI 20096288 A FI20096288 A FI 20096288A FI 20096288 A FI20096288 A FI 20096288A FI 20096288 A0 FI20096288 A0 FI 20096288A0
Authority
FI
Finland
Prior art keywords
formulations
methods
stem cells
culturing stem
culturing
Prior art date
Application number
FI20096288A
Other languages
Finnish (fi)
Swedish (sv)
Inventor
Kristiina Rajala
Marjo-Riitta Suuronen
Outi Hovatta
Heli Skottman
Original Assignee
Kristiina Rajala
Marjo Riitta Suuronen
Outi Hovatta
Heli Skottman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kristiina Rajala, Marjo Riitta Suuronen, Outi Hovatta, Heli Skottman filed Critical Kristiina Rajala
Priority to FI20096288A priority Critical patent/FI20096288A0/en
Publication of FI20096288A0 publication Critical patent/FI20096288A0/en
Priority to KR1020127017428A priority patent/KR20130009943A/en
Priority to PCT/FI2010/050980 priority patent/WO2011067465A1/en
Priority to AU2010326512A priority patent/AU2010326512A1/en
Priority to EP10800970A priority patent/EP2507360A1/en
Priority to JP2012541546A priority patent/JP2013512667A/en
Priority to CN2010800545573A priority patent/CN102906247A/en
Priority to CA2782296A priority patent/CA2782296A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FI20096288A 2009-12-04 2009-12-04 Formulations and Methods for Culturing Stem Cells FI20096288A0 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
FI20096288A FI20096288A0 (en) 2009-12-04 2009-12-04 Formulations and Methods for Culturing Stem Cells
KR1020127017428A KR20130009943A (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
PCT/FI2010/050980 WO2011067465A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
AU2010326512A AU2010326512A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
EP10800970A EP2507360A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
JP2012541546A JP2013512667A (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
CN2010800545573A CN102906247A (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
CA2782296A CA2782296A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI20096288A FI20096288A0 (en) 2009-12-04 2009-12-04 Formulations and Methods for Culturing Stem Cells

Publications (1)

Publication Number Publication Date
FI20096288A0 true FI20096288A0 (en) 2009-12-04

Family

ID=41462754

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20096288A FI20096288A0 (en) 2009-12-04 2009-12-04 Formulations and Methods for Culturing Stem Cells

Country Status (8)

Country Link
EP (1) EP2507360A1 (en)
JP (1) JP2013512667A (en)
KR (1) KR20130009943A (en)
CN (1) CN102906247A (en)
AU (1) AU2010326512A1 (en)
CA (1) CA2782296A1 (en)
FI (1) FI20096288A0 (en)
WO (1) WO2011067465A1 (en)

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US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
WO2009018453A1 (en) 2007-07-31 2009-02-05 Lifescan, Inc. Differentiation of human embryonic stem cells
EP2229434B1 (en) 2007-11-27 2011-09-07 Lifescan, Inc. Differentiation of human embryonic stem cells
KR102026622B1 (en) 2008-02-21 2019-09-30 얀센 바이오테크 인코포레이티드 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
US20100015711A1 (en) 2008-06-30 2010-01-21 Janet Davis Differentiation of Pluripotent Stem Cells
CA2742268C (en) 2008-10-31 2020-02-18 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
CN102333862B (en) 2008-10-31 2018-04-27 詹森生物科技公司 Differentiation of the human embryo stem cell to pancreatic endocrine pedigree
KR101774546B1 (en) 2008-11-20 2017-09-04 얀센 바이오테크 인코포레이티드 Pluripotent stem cell culture on micro-carriers
WO2010059778A1 (en) 2008-11-20 2010-05-27 Centocor Ortho Biotech Inc. Methods and compositions for cell attachment and cultivation on planar substrates
US10076544B2 (en) 2009-07-20 2018-09-18 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
JP6392496B2 (en) 2009-12-23 2018-09-19 ヤンセン バイオテツク，インコーポレーテツド Differentiation of human embryonic stem cells
AU2011223900A1 (en) 2010-03-01 2012-09-13 Janssen Biotech, Inc. Methods for purifying cells derived from pluripotent stem cells
JP6050225B2 (en) 2010-05-12 2016-12-21 ヤンセン バイオテツク，インコーポレーテツド Differentiation of human embryonic stem cells
PL2853589T3 (en) 2010-08-31 2018-05-30 Janssen Biotech, Inc Differentiation of human embryonic stem cells
MX355340B (en) 2010-08-31 2018-04-16 Janssen Biotech Inc Differentiation of human embryonic stem cells.
JP6148429B2 (en) * 2011-01-31 2017-06-14 協和発酵バイオ株式会社 Method for culturing human pluripotent stem cells
CA2860107C (en) 2011-12-22 2021-06-01 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
EP2823037A4 (en) 2012-03-07 2015-09-16 Janssen Biotech Inc Defined media for expansion and maintenance of pluripotent stem cells
ES2897649T3 (en) 2012-06-08 2022-03-02 Janssen Biotech Inc Differentiation of human embryonic stem cells into pancreatic endocrine cells
KR20150103203A (en) 2012-12-31 2015-09-09 얀센 바이오테크 인코포레이티드 Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
EP2938724B1 (en) 2012-12-31 2020-10-28 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
JP6557147B2 (en) 2012-12-31 2019-08-07 ヤンセン バイオテツク，インコーポレーテツド Differentiation of human embryonic stem cells into pancreatic endocrine cells using HB9 modulators
CA2913990C (en) 2013-05-30 2021-10-12 Ajinomoto Co., Inc. Medium for culturing stem cells
CN103432108A (en) * 2013-07-16 2013-12-11 江苏大学 Uses of N-3 unsaturated fatty acid in preparation of hematopoietic stem cell mobilization promoters
JP2017500893A (en) * 2013-12-20 2017-01-12 エッセンシャル ファーマシューティカル エルエルシー Medium for cell culture
EP3143127B1 (en) 2014-05-16 2021-07-14 Janssen Biotech, Inc. Use of small molecules to enhance mafa expression in pancreatic endocrine cells
WO2016088243A1 (en) * 2014-12-05 2016-06-09 株式会社ニコン Determination device, observation system, observation method, program for same, method for manufacturing cell, and cell
US20190167725A1 (en) * 2016-02-23 2019-06-06 University-Industry Cooperation Group Of Kyung Hee University Composition and method for improving efficacy of stem cells
MA45479A (en) 2016-04-14 2019-02-20 Janssen Biotech Inc DIFFERENTIATION OF PLURIPOTENT STEM CELLS IN ENDODERMAL CELLS OF MIDDLE INTESTINE
KR101896803B1 (en) * 2016-12-12 2018-09-07 서울대학교병원 Method for increasing the rate of inducing differentiation of human pluripotent stem cells to mesenchymal stem cells, and mesenchymal stem cells produced by thereof
CN110462025B (en) * 2017-01-23 2023-11-21 加拿大干细胞技术公司 Media and methods for enhancing stem cell survival and proliferation
CN110799639A (en) * 2017-06-27 2020-02-14 味之素株式会社 Culture medium containing riboflavin derivatives
SG11202003354VA (en) * 2017-10-13 2020-05-28 Boehringer Ingelheim Int Perfusion medium
JP2020162536A (en) * 2019-03-29 2020-10-08 興人ライフサイエンス株式会社 Medium composition for recombinant protein production increase
JP2023505988A (en) * 2019-12-09 2023-02-14 デウン ファーマシューティカル カンパニー リミテッド Method for producing mesenchymal stem cells from human pluripotent stem cells and mesenchymal stem cells produced thereby
WO2023286772A1 (en) * 2021-07-12 2023-01-19 三菱ケミカル株式会社 Cardiomyocyte production method
CN113832097B (en) * 2021-09-07 2024-02-20 生物岛实验室 Composition, serum-free feeder layer-free stem cell culture medium containing composition and application of serum-free feeder layer-free stem cell culture medium
CN115025122A (en) * 2022-06-25 2022-09-09 广东旺合生物科技有限公司 Application of autologous adipose-derived stem cells in preparation of anti-aging and anti-immunocompromised medicines or products
CN115896009A (en) * 2022-10-20 2023-04-04 王玥 Efficient low-cost culture medium for in-vitro differentiation of adipose-derived mesenchymal stem cells

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FI20075417A0 (en) * 2007-06-05 2007-06-05 Marjo-Riitta Suuronen Formulations and methods for culturing embryonic stem cells

Also Published As

Publication number Publication date
CN102906247A (en) 2013-01-30
AU2010326512A1 (en) 2012-06-28
EP2507360A1 (en) 2012-10-10
KR20130009943A (en) 2013-01-24
CA2782296A1 (en) 2011-06-09
WO2011067465A1 (en) 2011-06-09
JP2013512667A (en) 2013-04-18

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