ES2108322T3 - Procedimiento para la determinacion colorimetrica de un analito mediante (alcohol bencilico)-deshidrogenasa y un indicador de redox cromogeno. - Google Patents

Procedimiento para la determinacion colorimetrica de un analito mediante (alcohol bencilico)-deshidrogenasa y un indicador de redox cromogeno.

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Publication number
ES2108322T3
ES2108322T3 ES94105140T ES94105140T ES2108322T3 ES 2108322 T3 ES2108322 T3 ES 2108322T3 ES 94105140 T ES94105140 T ES 94105140T ES 94105140 T ES94105140 T ES 94105140T ES 2108322 T3 ES2108322 T3 ES 2108322T3
Authority
ES
Spain
Prior art keywords
colorimetric determination
analyte
deshidrogenase
procedure
benzyl alcohol
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94105140T
Other languages
English (en)
Inventor
Joachim Hoenes
Volker Unkrig
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2108322T3 publication Critical patent/ES2108322T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C255/00—Carboxylic acid nitriles
    • C07C255/01—Carboxylic acid nitriles having cyano groups bound to acyclic carbon atoms
    • C07C255/24—Carboxylic acid nitriles having cyano groups bound to acyclic carbon atoms containing cyano groups and singly-bound nitrogen atoms, not being further bound to other hetero atoms, bound to the same saturated acyclic carbon skeleton
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D231/00—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings
    • C07D231/02—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings
    • C07D231/10—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D231/14—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D231/44—Oxygen and nitrogen or sulfur and nitrogen atoms
    • C07D231/46—Oxygen atom in position 3 or 5 and nitrogen atom in position 4
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D513/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00
    • C07D513/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains two hetero rings
    • C07D513/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6561—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y10T436/173845—Amine and quaternary ammonium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y10T436/173845—Amine and quaternary ammonium
    • Y10T436/174614—Tertiary amine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y10T436/173845—Amine and quaternary ammonium
    • Y10T436/175383—Ammonia

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

LA INVENCION SE REFIERE A UN PROCEDIMIENTO PARA LA DETERMINACION COLORIMETRICA DEL NADH POR MEDIO DE OXIDACION ENZIMATICA CON BENCILALCOHOL DE HIDROGENASA BAJO REDUCCION DE UN COMPUESTO NITROSO AROMATICO DE LA FORMULA I A UNA QUINONDIIMINA COLOREADA EN DONDE R1 ES HIDROGENO, HALOGENO, HIDROXI, ALCOXI, ARILOXI, ALQUILTIO, ARILTIO, ARIL O ALQUILENO EVENTUALMENTE SUSTITUIDO POR CARBOXI, PO3H2, DIALQUILFOSFINIL O SO3H, AMINO EVENTUALMENTE SUSTITUIDO UNA O DOS VECES POR ALQUIL, QUE EVENTUALMENTE PUEDE SER SUSTITUIDO DE NUEVO POR HIDROXI, PO3H2, SO3H O CARBOXI Y R SIGNIFICA UN RESTO GENERADOR DE COLOR POR MEDIO DE CONJUGACION DE ELECTRONES CON UN SISTEMA QUINONDIIMINA. LA INVENCION SE REFIERE ADEMAS A UN MEDIO CORRESPONDIENTE ASI COMO A UN COMPUESTO NITROSO CROMOGENO DE LA FORMULA I ASI COMO SU UTILIZACION PARA LA DETERMINACION COLORIMETRICA ENZIMATICA DE UNA MUESTRA.
ES94105140T 1993-04-08 1994-03-31 Procedimiento para la determinacion colorimetrica de un analito mediante (alcohol bencilico)-deshidrogenasa y un indicador de redox cromogeno. Expired - Lifetime ES2108322T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4311460A DE4311460A1 (de) 1993-04-08 1993-04-08 Verfahren zur kolorimetrischen Bestimmung eines Analyten mittels Benzylalkoholdehydrogenase und einem chromogenen Redoxindikator

Publications (1)

Publication Number Publication Date
ES2108322T3 true ES2108322T3 (es) 1997-12-16

Family

ID=6484981

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94105140T Expired - Lifetime ES2108322T3 (es) 1993-04-08 1994-03-31 Procedimiento para la determinacion colorimetrica de un analito mediante (alcohol bencilico)-deshidrogenasa y un indicador de redox cromogeno.

Country Status (7)

Country Link
US (1) US5445943A (es)
EP (1) EP0619374B1 (es)
JP (1) JP3001368B2 (es)
AT (1) ATE156807T1 (es)
DE (2) DE4311460A1 (es)
DK (1) DK0619374T3 (es)
ES (1) ES2108322T3 (es)

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US7399850B2 (en) * 1999-06-18 2008-07-15 Ceres, Inc. Sequence-determined DNA fragments encoding AP2 domain proteins
US7420049B2 (en) * 1999-06-18 2008-09-02 Ceres, Inc. Sequence-determined DNA fragments encoding AP2 domain proteins
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US20060194958A1 (en) * 1999-11-10 2006-08-31 Nickolai Alexandrov Sequence-determined DNA fragments encoding AN1-like zinc finger proteins
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US7420046B2 (en) * 1999-11-10 2008-09-02 Ceres, Inc. Sequence-determined DNA fragments encoding RNA polymerase proteins
US20060235218A1 (en) * 1999-12-08 2006-10-19 Nickolai Alexandrov Sequence-determined DNA fragments encoding thioredoxin proteins
US7355026B2 (en) * 2000-01-27 2008-04-08 Ceres, Inc. Sequence-determined DNA fragments encoding SRF-type transcription factor proteins
US20060252920A1 (en) 2001-08-10 2006-11-09 Nickolai Alexandrov Sequence-determined DNA fragments encoding cyclopropyl isomerase proteins
US7691991B2 (en) * 2000-04-17 2010-04-06 Ceres, Inc. Sequence-determined DNA fragments encoding cytochrome P450 proteins
US10106586B2 (en) 2000-08-07 2018-10-23 Ceres, Inc. Sequence-determined DNA fragments encoding peptide transport proteins
US7390893B2 (en) * 2000-08-07 2008-06-24 Ceres, Inc. Sequence-determined DNA fragments encoding peptide transport proteins
US9085771B2 (en) 2001-01-03 2015-07-21 Ceres, Inc. Sequence-determined DNA fragments with regulatory functions
US9024004B2 (en) 2000-08-31 2015-05-05 Ceres, Inc. Sequence-determined DNA fragments encoding acetohydroxyacid synthase proteins
US7608441B2 (en) 2000-08-31 2009-10-27 Ceres, Inc. Sequence-determined DNA fragments encoding sterol desaturase proteins
US7604971B2 (en) * 2000-10-19 2009-10-20 Ceres, Inc. Sequence-determined DNA fragments encoding UBIE/COQ5 methyltransferase family proteins
US20060270842A1 (en) * 2000-10-19 2006-11-30 Nickolai Alexandrov Sequence-determined DNA fragments encoding sterol desaturase proteins
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US7385046B2 (en) * 2001-01-03 2008-06-10 Ceres, Inc. Sequence-determined DNA fragments encoding ethylene responsive element binding proteins
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US20060217540A1 (en) * 2001-04-02 2006-09-28 Nickolai Alexandrov Sequence-determined DNA fragments encoding AP2 domain proteins
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US9068173B2 (en) 2002-06-17 2015-06-30 Ceres, Inc. Sequence-determined DNA fragments encoding trehalose-6P phosphatase proteins
US20060194959A1 (en) * 2002-07-15 2006-08-31 Nickolai Alexandrov Sequence-determined DNA fragments encoding SRF-type transcription factors
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Also Published As

Publication number Publication date
JPH0710814A (ja) 1995-01-13
EP0619374B1 (de) 1997-08-13
ATE156807T1 (de) 1997-08-15
US5445943A (en) 1995-08-29
EP0619374A2 (de) 1994-10-12
DE4311460A1 (de) 1994-10-13
DE59403694D1 (de) 1997-09-18
DK0619374T3 (da) 1998-03-30
JP3001368B2 (ja) 2000-01-24
EP0619374A3 (de) 1995-09-06

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