CN217304797U - Dual-wavelength multi-channel bioaerosol analyzer - Google Patents
Dual-wavelength multi-channel bioaerosol analyzer Download PDFInfo
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- CN217304797U CN217304797U CN202123418727.6U CN202123418727U CN217304797U CN 217304797 U CN217304797 U CN 217304797U CN 202123418727 U CN202123418727 U CN 202123418727U CN 217304797 U CN217304797 U CN 217304797U
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Abstract
The invention discloses a dual-wavelength multi-channel bioaerosol analyzer, which comprises three waveband light sources, namely 635nm, 260nm-290nm and 340nm-380 nm; two fluorescence detection channels are 310-400 nm and 420-650 nm; a particle shape detection channel. According to the invention, through the design of double ultraviolet light excitation, double fluorescence detection channels and particle shape detection, a multi-parameter discrimination basis is provided for bioaerosol detection, the accuracy of real-time early warning of organisms is greatly improved, and the type of biological particles can be discriminated by combining multi-parameter modeling.
Description
Technical Field
The invention relates to the technical field of air bioaerosol detection, in particular to a dual-wavelength multi-channel bioaerosol analyzer.
Background
Releasing bioaerosols is the primary mode of bioterrorism attack. How to realize the distinguishing and real-time monitoring of the bioaerosol has great significance in the field of bioterrorism prevention, and is one of the hot spots of research in various countries in recent years.
For the detection of microorganisms in the air, the traditional analysis method is a biological culture method, namely, an aerosol sample in the air is collected into a culture medium to be cultured for several hours to several days to form a colony unit, so as to obtain reliable biological information in the aerosol. Then, a laser-induced fluorescence technology based on biological body fluorescence characteristics appears, and the detection and identification of the biological aerosol are completed by inducing the biological substances to generate specific fluorescence spectra through laser.
The bio-aerosol can generate fluorescence because it contains bioactive fluorescent materials, such as pollen, bacteria, etc., which contain active substances capable of generating fluorescence. The fluorescence effect is the excited transition of molecules and outer electrons of atoms to radiate photons, and has a direct relation with the energy level distribution and the space structure of the molecules. It is considered that the fluorescence spectrum of the biological particle is formed by overlapping the fluorescence spectra of the fluorescent molecules therein. The main characteristic peak of its fluorescence is therefore believed to be derived from the internal representative fluorescent molecule. Typical bioluminescent molecules include various amino acids, vitamins, coenzymes, etc., and these molecules of light are widely present in atmospheric aerosols of various bacteria, fungi, etc. Wherein the 280nm laser has high effect on the fluorescence excitation of tryptophan, and the 365nm laser has high effect on the fluorescence excitation of NADH (nicotinamide adenine dinucleotide). The fluorescence detection of the biological particles is usually excited by these two lasers.
At present, some aerosol detection devices based on ultraviolet induced fluorescence are designed, but basically, a single-waveband 405nm laser diode is used as exciting light, single excitation and single detection are carried out, the exciting efficiency is low, detection parameters are few, false alarm is easy to generate, and the accuracy of biological early warning is low.
Disclosure of Invention
In view of the above, the present invention provides a dual-wavelength multi-channel bioaerosol analyzer, which uses dual-band ultraviolet light to excite bioluminescence, thereby improving fluorescence excitation efficiency, and designs dual fluorescence detection channels, thereby increasing fluorescence information of particles, using a multi-quadrant detector to detect particle shapes, and using multi-parameter information to model biological particles, thereby improving the accuracy of the device for biological pre-warning.
A dual-wavelength multi-channel bioaerosol analyzer comprises a sealed shell, a light source module, a fluorescence detection module, a gas circuit module and a control module;
a detection cavity is formed in the sealed shell;
the light source module is fixed on the sealed shell and comprises a visible laser module, a first ultraviolet light source module and a second ultraviolet light source module which are sequentially arranged at intervals along the vertical direction; the optical axis of the visible laser module, the first optical axis of the first ultraviolet light source module and the second optical axis of the second ultraviolet light source module are all arranged along the horizontal direction and are all perpendicular to the air inlet flow column of the air path module; in the projection of a horizontal plane, the first optical axis and the second optical axis are symmetrically arranged by taking the optical axis as a symmetry axis; the center wavelength of the visible laser module is 635 nm; the light-emitting wavelength of the first ultraviolet light source module is 260-290 nm; the light-emitting wavelength of the second ultraviolet light source module is 340nm-380 nm;
the fluorescence detection module is fixed on the sealed shell and comprises a concave reflector, a diaphragm g, a detection cavity, a dichroic mirror, a fluorescence detection channel I and a fluorescence detection channel II; the concave reflector is positioned in the detection cavity and is arranged opposite to the detection cavity, and the central optical axis of the concave reflector, the airflow column of the air inlet of the air path module and the optical axis of the visible laser module are all vertical to each other; the diaphragm g is positioned at the entrance of the detection cavity, has the same optical axis with the concave reflector and is vertical to the optical axis of the visible laser module; the dichroic mirror is positioned in the detection cavity, and the mirror surface of the dichroic mirror is intersected with the central optical axis and arranged at an angle of 45 degrees; the fluorescence detection channel is positioned in the reflection area of the dichroic mirror; the two fluorescence channels are positioned in the transmission area of the dichroic mirror;
the control module comprises a power module, a time sequence module and a signal processing module; the power supply module is fixed on the surface of the bottom plate of the sealed shell and is electrically connected with the light source module and the fluorescence detection module; the time sequence module is in signal connection with the first ultraviolet light source module and the second ultraviolet light source module and is used for controlling the flashing time sequences of the first ultraviolet light source module and the second ultraviolet light source module; the signal processing module is in signal connection with the fluorescence detection module and is used for processing and converting voltage signals detected by the fluorescence detection channel I and the fluorescence detection channel II.
Further, the particle shape detection device also comprises a particle shape detection module packaged in the black lens cone f;
the particle shape detection module comprises a diaphragm f, an optical filter f, a focusing mirror f and a four-quadrant detector which are sequentially arranged; the diaphragm f, the optical filter f and the focusing mirror f are coaxially arranged with the visible laser diode; the diaphragm f and the laser diaphragm a are symmetrically arranged by taking the central axis of the air inlet as a symmetric axis; the focusing mirror f converges the scattered light of the visible laser diode to the four-quadrant detector; the four-quadrant detector is positioned on the back focus of the focusing mirror f;
the signal processing module is in signal connection with the four-quadrant detector, processes and converts voltage signals detected by the four-quadrant detector, and realizes information storage of particle size distinguishing, counting, particle shape and fluorescence excitation interval of each particle.
Further, the surface of the optical filter f is plated with an antireflection film with a wavelength of 635 nm.
Furthermore, the included angle theta between the first optical axis and the optical axis is 10-20 degrees.
Further, the visible laser module comprises a visible laser diode, a focusing mirror a, an optical filter a and a diaphragm a which are packaged in the black lens barrel a; the focusing mirror a is made of an ultraviolet light-transmitting material and is plated with an antireflection film, wherein the focusing mirror a, the optical filter a and the diaphragm a are sequentially arranged and share the same optical axis with laser emitted by the visible laser diode;
the first ultraviolet light source module comprises an ultraviolet xenon lamp light source b, a focusing mirror b, an optical filter b and a diaphragm b, wherein the ultraviolet xenon lamp light source b, the focusing mirror b, the optical filter b and the diaphragm b are packaged in a black lens barrel b; the ultraviolet xenon lamp light source b is arranged at the bottom of the lens cone b; the focusing mirror b, the optical filter b and the diaphragm b are sequentially arranged and have the same optical axis with the laser emitted by the ultraviolet xenon lamp light source b;
the second ultraviolet light source module comprises an ultraviolet xenon lamp light source c, a focusing lens c, an optical filter c and a diaphragm c, which are packaged in a black lens barrel c; the ultraviolet xenon lamp light source c is arranged at the bottom of the lens barrel c; the focusing lens c, the optical filter c and the diaphragm c are sequentially arranged and have the same optical axis with the laser emitted by the ultraviolet xenon lamp light source c;
and the time sequence module is in signal connection with the visible laser diode, the ultraviolet xenon lamp light source b and the ultraviolet xenon lamp light source c.
Further, the focusing mirror b and the focusing mirror c are both made of ultraviolet light-transmitting materials and are coated with antireflection films.
Further, the first fluorescence detection channel comprises an optical filter d, a focusing mirror d and a detector d; the optical filter d is positioned in the reflection area of the dichroic mirror; the focusing lens d is made of an ultraviolet light-transmitting material and is plated with an antireflection film, and the detector d is positioned on the back focus of the focusing lens d; the optical filter d, the focusing lens d and the detector d are sequentially arranged and coaxial;
the fluorescence detection channel II comprises an optical filter e, a focusing mirror e and a detector e, wherein the optical filter e, the focusing mirror e and the detector e are sequentially arranged and coaxial; the optical filter e is positioned in the transmission area of the dichroic mirror, and the surface of the optical filter e is plated with an anti-reflection film; the detector e is positioned on the back focus of the focusing lens e;
and the signal processing module processes and converts the voltage signals detected by the detector e and the detector d.
Further, the detector e is a photomultiplier tube.
Further, the gas circuit module comprises a sample introduction device, a filter, a gas pump, a first flow control valve, a second flow control valve and a needle valve;
the sample feeding device comprises a particle cutter, a sheath flow gas path, a sample flow gas tube, a sheath flow nozzle and a gas inlet; the particle cutter is mounted to the sheath flow nozzle; the sheath flow nozzle is hermetically arranged at the air inlet, and the outside of the sheath flow nozzle is made of stainless steel materials and is funnel-shaped; the sample gas flow pipe is inserted into the center of the sheath flow nozzle and coaxially arranged, the diameter of the sample gas flow pipe is 0.7mm, the periphery of the sample gas flow pipe is wrapped by sheath flow of a sheath flow gas circuit, and the diameter of the sheath flow gas circuit is 1 mm;
the filter is connected between the air outlet of the detection cavity and the air pump; the air outlet of the air pump is divided into two paths: one path is connected with an exhaust port of the sealed shell through the first flow control valve, and the other path is connected with the sheath flow nozzle through the second flow control valve; the needle valve is connected between the air pump sheath flow air outlet and the detection cavity through a thin air pipe.
Further, the filter is characterized in that the aperture of the filter pores of the filter element of the filter is 0.01 μm; the flow rate of the air pump is 3L/min.
Has the advantages that:
according to the invention, through the design of double ultraviolet light excitation, double fluorescence detection channels and particle shape detection, biological fluorescence is excited, the fluorescence excitation efficiency is improved, the biological aerosol in the air can be monitored and early warned in real time, the particle size, the shape and the concentration are included, a multi-parameter discrimination basis is provided for biological aerosol detection, the type of the modeled biological particles is warned through multi-parameter analysis and judgment, and the accuracy of real-time early warning of the organisms is greatly improved.
Drawings
Fig. 1 is a schematic structural diagram of a dual-wavelength multi-channel bioaerosol analyzer according to the present invention.
Fig. 2 is a top view of the dual wavelength multi-channel bioaerosol analyzer of fig. 1.
Fig. 3 is a schematic diagram of the gas path structure of the dual-wavelength multi-channel bioaerosol analyzer.
Fig. 4 is a schematic diagram of the light source position of a dual wavelength multi-channel bioaerosol analyzer.
Detailed Description
The invention is described in detail below by way of example with reference to the accompanying drawings.
The embodiment of the invention provides a dual-wavelength multichannel bioaerosol analyzer, which comprises: the device comprises a sealed shell 53, a light source module, a fluorescence detection module, a particle shape detection module, an air channel module and a hardware control module.
The entire sealed housing 53 is made of a light-tight 304 stainless steel material, and all inner surfaces thereof are blackened by oxidation to prevent the material from reflecting stray light and to electrostatically adsorb particles. The sealing housing 53 is used to seal and fix each module, so that the aerosol to be detected is introduced, a closed environment is formed, and the influence of external environment light and particles on the detection result is prevented.
The light source module comprises a visible laser module 31 and two ultraviolet light source modules 2 and 28; the optical axes of the three light sources in the horizontal direction are all kept horizontal and are vertical to the air flow 60 at the air inlet 59 of the air path module; the optical axis of the visible laser is vertical to the outer shell, and the two ultraviolet light sources are symmetrically arranged at two sides of the visible laser at an included angle theta; visible laser is located the position that the top is close to air inlet 59 on the vertical direction, and the interval of ultraviolet light source 2 and ultraviolet light source two 28 is located the visible laser below with L, calculates the delay time sequence through the interval L of accurate flow and optical axis, and the lighting of accurate control ultraviolet light source. The spacing L of the visible laser module 31, the first uv light source module 2 and the second uv light source module 28 is calculated from the actual detection capability performance parameters to ensure that the dead time is controlled to a minimum.
A control module 43 including a power module 44, a timing module 45, and a signal processing module 46; the power supply module 44 is used for converting the input 220V voltage into a DC power supply voltage required by each module in the instrument, and is fixed on the surface of the bottom plate of the outer shell of the detection cavity 10; the time sequence module 45 is used for controlling the flashing time sequences of the two xenon lamps, when particles enter the detection chamber 10, the detector e21 triggers the time sequence controller to work after detecting a side scattering signal of the laser diode 54, time delay is calculated according to the sample flow and the distance L between ultraviolet light beams, and then the ultraviolet xenon light source b1 and the ultraviolet xenon light source c29 are respectively controlled to flash once when the particles arrive according to the time delay, so that the fluorescent information excited by each ultraviolet wave band of each particle is ensured to be detected; the signal processing module 46 is configured to process and convert voltage signals detected by the detector d24, the detector e21, and the four-quadrant detector 13, wherein the number of pulses is recorded by the detector e21 to count the number of pulses, the four-quadrant detector 13 determines the particle size by a voltage value, and calibrates the voltage corresponding to the particle size by a PSL (PolyStyrene Latex) pellet with a standard particle size, so as to store information such as the particle size distinction, the count, the particle shape, and the fluorescence excitation interval of each particle, and transmits a signal processing result to the upper computer 48 to display the signal processing result by the signal line 47, that is, the RS 232-USB serial port line, and the database is established by multiple parameters of the sample to perform algorithm identification and determination, so that the species of biological particles can be preliminarily distinguished, and the accuracy of real-time biological early warning is greatly improved.
The visible laser module 31 comprises a visible laser diode 54, a focusing mirror a32, a filter a30, a diaphragm a 7; the whole module is packaged in a black lens barrel a55, and has good air tightness so as to ensure that the detection cavity 10 is airtight; the diode is a laser diode 54 with 635nm as a central wavelength, and is used for generating scattered light through particles, detecting the side scattered light to judge the particle count and the particle size, and triggering an ultraviolet light source to light, wherein the Mie scattering intensity of the diode is higher than that of the near infrared light, the scattered light in the waveband can be detected by a detector e21 of a fluorescence channel II 17, and a special detector is not needed, so that the complexity of the structure is reduced; the focusing lens a32 is made of ultraviolet transparent material and is coated with an antireflection film, the passing rate reaches 95%, and the focusing lens a32 is positioned in front of the visible laser diode 54 and has the same optical axis with the laser and is used for collimating the visible laser; the optical filter a30 is positioned in front of the focusing mirror and is coaxial with the focusing mirror, and is used for improving the monochromaticity of the 635nm laser; the diaphragm a7 is positioned in front of the focusing lens and coaxial with the optical filter and is used for filtering stray light.
The ultraviolet light source module I2 comprises an ultraviolet xenon lamp light source b1, a focusing mirror b3, an optical filter b5 and a diaphragm b 6; the whole module is packaged in a black lens barrel b56, and has good air tightness so as to ensure that the detection cavity 10 is airtight; the ultraviolet xenon lamp light source b1 is arranged at the bottom of the lens barrel b 56; the focusing mirror b3 is made of an ultraviolet light-transmitting material and is coated with an anti-reflection film, the passing rate reaches 95%, and the focusing mirror b3 is positioned in front of the ultraviolet xenon lamp light source b1 and has the same optical axis with the emitted laser and is used for collimating the laser emitted by the ultraviolet xenon lamp light source b 1; the optical filter b5 is positioned in front of the focusing mirror and coaxial with the focusing mirror and is used for filtering out wave bands except for the xenon lamp light source 1 so as to ensure the accuracy and monochromaticity of a 260-290 nm excitation light source, the 260-290 nm light source has high fluorescence excitation efficiency on glutamic acid, and the detection sensitivity of bioaerosol can be improved; the diaphragm b6 is positioned in front of the focusing mirror and coaxial with the optical filter and is used for filtering stray light.
The second ultraviolet light source module 28 comprises an ultraviolet xenon lamp light source c29, a focusing mirror c27, an optical filter c26 and a diaphragm c 25; the whole module is packaged in a black lens barrel c57, and has good air tightness so as to ensure that the detection cavity 10 is airtight; the ultraviolet xenon lamp light source c29 is arranged at the bottom of the lens barrel c 57; the focusing lens c27 is made of ultraviolet light-transmitting material and is plated with an anti-reflection film, the passing rate reaches 95%, the focusing lens is positioned in front of the ultraviolet xenon lamp light source c29 and is coaxial with the emitted laser, and the focusing lens is used for collimating the laser emitted by the ultraviolet xenon lamp light source c 29; the optical filter c26 is positioned in front of the focusing mirror and coaxial with the focusing mirror, and is used for filtering the wave bands of the xenon lamp light source c29 except for the wave bands of 340-380 nm so as to ensure the accuracy and monochromaticity of the output light source of 340-380 nm, and the 340-380 nm light source has high fluorescence excitation efficiency on NADH, so that the detection sensitivity of bioaerosol can be improved; and a diaphragm c25 is positioned in front of the focusing lens and is coaxial with the optical filter and used for filtering stray light. The light-emitting wavelength of the ultraviolet xenon lamp light source b1 is 260nm-290 nm; the light emitting wavelength of the ultraviolet xenon lamp light source c29 is 340nm-380 nm.
The fluorescence detection module comprises a concave reflector 9, a diaphragm g16, a detection cavity 17, a dichroic mirror 18, a first fluorescence detection channel 49 and a second fluorescence detection channel 50; the surface of the concave reflector 9 is plated with an ultraviolet enhanced aluminum film to ensure that the reflectivity to 310 nm-650 nm is more than 85%, the concave reflector 9 is positioned behind the detection cavity 10, and the central optical axis 58 of the concave reflector 9 is perpendicular to the optical axes of the airflow column 60 of the air inlet 59 of the air channel module and the visible laser 31, and is used for collecting side scattered light and reflecting the light into the detection cavity 17; the diaphragm g16 is positioned at the entrance of the detection cavity 17, has the same optical axis with the concave reflector 9, is vertical to the optical axis of the visible laser 31, and is used for filtering out stray light; the dichroic mirror 18 is positioned behind the diaphragm, the mirror surface of the dichroic mirror intersects with the central optical axis 58 of the concave reflecting mirror and is arranged at an angle of 45 degrees, the dichroic mirror is used for dividing scattered light into two different waveband intervals through transmission and reflection, the dichroic mirror has high reflectivity for a waveband of 310-400 nm and has high transmissivity for a waveband of 420-650 nm; the first fluorescence detection channel 49 is located in the reflection area of the dichroic mirror 18 and used for receiving a fluorescence signal of 310-400 nm generated by excitation; the second fluorescence channel 50 is located in the transmission region of the dichroic mirror 18 and used for counting and particle size recording by detecting scattered light generated by the visible 280nm laser diode 54, triggering the ultraviolet xenon light source b1 and the ultraviolet xenon light source c29 to flash sequentially by combining the signal with time delay control, and receiving fluorescence signals of 420-650 nm generated by excitation.
The first fluorescence detection channel 49 comprises a filter d22, a focusing mirror d23 and a detector d 24; the optical filter d22 is positioned in the reflection area of the dichroic mirror 18, is placed at an angle of 45 degrees with the dichroic mirror, and is coated with an antireflection film with a wavelength band of 310-400 nm on the surface for further filtering, so that only light with a wavelength of 310-400 nm passes through; the focusing lens d23 is positioned behind the optical filter d22, is coaxial with the optical filter d22, is made of ultraviolet light-transmitting materials and is coated with an antireflection film, the passing rate reaches 95%, and the focusing lens d23 is used for converging light on the detector d 24; the detector d24 is located at the back focus of the focusing lens d23, is coaxial with the focusing lens d23, adopts a photomultiplier tube with high magnification, improves the detection sensitivity, and is used for detecting fluorescent signals of 310-400 nm.
The second fluorescence detection channel 50 comprises a filter e19, a focusing mirror e20 and a detector e 21; the optical filter e19 is positioned in the transmission area of the dichroic mirror 18, is placed at an angle of 45 degrees with the dichroic mirror, and is coated with an antireflection film with a waveband of 420-650 nm on the surface for further filtering, so that only light with a wavelength of 420-650 nm passes through; the focusing mirror e20 is positioned behind the optical filter e19, is coaxial with the optical filter e19, is made of an ultraviolet light-transmitting material and is coated with an anti-reflection film, the passing rate reaches 95%, and the focusing mirror e20 is used for converging light on the detector e 21; the detector e21 is located at the back focus of the focusing lens e20, is coaxial with the focusing lens e20, adopts a photomultiplier tube with high magnification, improves the detection sensitivity, and is used for detecting fluorescent signals of 420-650 nm.
According to the invention, through the design of double ultraviolet light excitation and double fluorescence detection channels, the biological fluorescence is excited, the fluorescence excitation efficiency is improved, the monitoring and early warning of the bioaerosol in the air can be realized in real time, the particle size, the shape and the concentration are included, and a multi-parameter discrimination basis is provided for bioaerosol detection.
The particle shape detection module comprises a diaphragm f15, an optical filter f14, a focusing mirror f12 and a four-quadrant detector 13; the whole module is packaged in a black lens cone 11, and has good air tightness so as to ensure that the detection cavity 10 is airtight; the diaphragm f15 is coaxial with the diaphragm a7 of the laser diode 54, and is symmetrically arranged with the laser diaphragm a7 by taking the airflow column 60 of the air inlet 59 of the air path module as a central optical axis, so as to filter out stray light; the optical filter f14 is positioned behind the diaphragm f15, is coaxial with the diaphragm f15, is coated with an antireflection film with a 635nm waveband on the surface, and is used for allowing only forward scattered light of the 635nm laser diode 54 to pass through; the focusing mirror f12 is positioned behind the filter f14 and coaxial with the filter f14 and is used for converging the scattered light of the laser diode 54 on the four-quadrant detector 13; the four-quadrant detector 13 is located at the back focal point of the focusing mirror f12 and is used for detecting the scattered light of the laser diode 54 after passing through the particles, asymmetry of the shape of the particles can be caused, the root mean square variation of the average value is calculated through the intensity distribution of four quadrants of the detector, an aspheric factor AF is obtained, the AF corresponding to an absolute sphere is 0, and the thin rod-shaped AF value approaches 100, so that the particle size shapes are distinguished. Through the detection to particle shape, judge biological granule kind, improved the degree of accuracy to the real-time early warning of biology greatly.
The gas circuit module comprises a sample injection device 8, a filter 41, an air pump 39, a flow control valve 38, a flow control valve 42 and a needle valve 52; the sample feeding device 8 consists of a particle cutter 33, a sheath flow air path 35, a sample flow air pipe 36, a sheath flow nozzle 51 and an air inlet 59;
the particle cutter 33 is installed on the sheath flow nozzle 51, and only 1-10 μm particles pass through the particle cutter by the design of two-stage impact type filtration;
the sheath flow nozzle 51 is made of stainless steel material and is funnel-shaped, and is arranged on the air inlet 59 in a sealing way; the aerosol particle size cutting device is used for compressing aerosol after particle size cutting to generate stable fine airflow, so that particles can pass through a laser detection area individually, and the detection precision is improved;
sample gas flow pipe 36 inserts the installation from the sheath flow nozzle 51 centre, and the diameter is 0.7mm, is flowed the parcel by the sheath of sheath gas circuit 35 all around, and the diameter is 1mm, and two kinds of air currents are joined in the air inlet nozzle export, reduce the particulate matter and overlap through the detection zone, improve and detect the precision.
The filter 41 is positioned at the air outlet 40 below the detection cavity 10 and is hermetically connected with the air pipe, and the filter element of the filter is only particles with the particle size of 0.01 mu m and is used for filtering all particulate matters, avoiding polluting the air pump and ensuring the cleanliness of subsequent air flow; the air pump 39 is positioned behind the filter, a 3L/min micro-diaphragm air pump is selected, an air inlet of the air pump is hermetically connected with the filter through an air pipe, an air outlet of the air pump is divided into two paths through an air path joint, one path is used for exhausting air, the other path is used for providing sheath flow, and the air pump is used for providing power for an air path system and sucking the environmental aerosol into the detection cavity 10; the air outlet of the air pump 39 is connected with the instrument air outlet 37 through the flow control valve 38 and is used for monitoring and controlling the exhaust flow in real time to keep the stability of the flow of the whole air path system; one end of the flow control valve 42 is connected with the air outlet of the air pump 39 by an air pipe, the other end is connected with the sheath inflow port of the sheath flow nozzle 51 by the air pipe, the flow control valve is used for monitoring and controlling the sheath flow in real time to keep the stability of the sheath flow, and the flow control valve 38 is matched for controlling to ensure that the sheath flow is 2.2L/min and the sample flow is 0.3L/min, so that the sample flow and the sheath flow are laminar;
the needle valve 52 is connected between the sheath flow outlet of the air pump and the detection cavity 10 of the instrument by a thin air pipe, and is used for blowing the detection cavity 10 in real time by using tiny clean air flow, so that the cleanliness of the cavity is kept, and particles are prevented from being deposited on the surface of the cavity to cause pollution.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A dual-wavelength multi-channel bioaerosol analyzer is characterized by comprising a sealed shell (53), a light source module, a fluorescence detection module, an air channel module and a control module (43);
a detection cavity (10) is formed in the sealed shell (53);
the light source module is fixed on the sealed shell and comprises a visible laser module (31), a first ultraviolet light source module (2) and a second ultraviolet light source module (28) which are sequentially arranged at intervals along the vertical direction; an optical axis (61) of the visible laser module (31), a first optical axis (62) of the first ultraviolet light source module (2) and a second optical axis (63) of the second ultraviolet light source module (28) are all arranged along the horizontal direction and are all perpendicular to an air inlet flow column (60) of the air path module; in the projection of a horizontal plane, a first optical axis (62) and a second optical axis (63) are symmetrically arranged with an optical axis (61) as a symmetry axis; the center wavelength of the visible laser module (31) is 635 nm; the light-emitting wavelength of the first ultraviolet light source module (2) is 260nm-290 nm; the light-emitting wavelength of the second ultraviolet light source module (28) is 340nm-380 nm;
the fluorescence detection module is fixed on the sealed shell and comprises a concave reflector (9), a diaphragm g (16), a detection cavity (17), a dichroic mirror (18), a fluorescence detection channel I (49) and a fluorescence detection channel II (50); the concave reflector (9) is positioned in the detection cavity (10) and is arranged opposite to the detection cavity (17), and the central optical axis (58) of the concave reflector (9), the airflow column (60) of the air inlet (59) of the air path module and the optical axis of the visible laser module (31) are vertical to each other; the diaphragm g (16) is positioned at the entrance of the detection cavity (17), has the same optical axis with the concave reflector (9), and is vertical to the optical axis of the visible laser module (31); the dichroic mirror (18) is positioned in the detection cavity (17), and the mirror surface of the dichroic mirror (18) is intersected with the central optical axis (58) and arranged at an angle of 45 degrees; the first fluorescence detection channel (49) is positioned in the reflection area of the dichroic mirror (18); the second fluorescence detection channel (50) is positioned in the transmission area of the dichroic mirror (18);
the control module (43) comprises a power supply module (44), a timing module (45) and a signal processing module (46); the power supply module (44) is fixed on the surface of the bottom plate of the sealed shell and is electrically connected with the light source module and the fluorescence detection module; the time sequence module (45) is in signal connection with the first ultraviolet light source module (2) and the second ultraviolet light source module (28) and is used for controlling the flashing time sequence of the first ultraviolet light source module (2) and the second ultraviolet light source module (28); the signal processing module is in signal connection with the fluorescence detection module and is used for processing and converting voltage signals detected by the fluorescence detection channel I and the fluorescence detection channel II.
2. The dual wavelength multi-channel bioaerosol analyzer of claim 1, further comprising a particle shape detection module enclosed in a black column f (11);
the particle shape detection module comprises a diaphragm f (15), an optical filter f (14), a focusing mirror f (12) and a four-quadrant detector (13) which are sequentially arranged; the diaphragm f (15), the optical filter f (14) and the focusing mirror f (12) are all arranged coaxially with the visible laser diode (54); the diaphragm f (15) and the laser diaphragm a (7) are symmetrically arranged by taking the central axis of the air inlet (59) as a symmetrical axis; the focusing mirror f (12) converges scattered light of the visible laser diode (54) to the four-quadrant detector (13); the four-quadrant detector (13) is positioned on the back focus of the focusing mirror f (12);
the signal processing module is in signal connection with the four-quadrant detector, processes and converts voltage signals detected by the four-quadrant detector, and realizes information storage of particle size distinguishing, counting, particle shape and fluorescence excitation interval of each particle.
3. The dual-wavelength multi-channel bioaerosol analyzer of claim 2, wherein the filter f is coated with an anti-reflection film with a wavelength of 635 nm.
4. A dual wavelength multi-channel bioaerosol analyzer as claimed in claim 1, wherein the angle θ between the first optical axis (62) and the optical axis (61) is between 10 ° and 20 °.
5. The dual-wavelength multichannel bioaerosol analyzer as claimed in claim 1, wherein the visible laser module (31) comprises a visible laser diode (54), a focusing mirror a (32), a filter a (30), a diaphragm a (7) packaged in a black lens barrel a (55); the focusing lens a (32) is made of ultraviolet light-transmitting materials and is coated with an antireflection film, wherein the focusing lens a (32), the optical filter a (30) and the diaphragm a (7) are sequentially arranged and have the same optical axis with laser emitted by the visible laser diode (54);
the first ultraviolet light source module (2) comprises an ultraviolet xenon lamp light source b (1), a focusing mirror b (3), an optical filter b (5) and a diaphragm b (6), wherein the ultraviolet xenon lamp light source b (1), the focusing mirror b (3), the optical filter b (5) and the diaphragm b (6) are packaged in a black lens barrel b (56); the ultraviolet xenon lamp light source b (1) is arranged at the bottom of the lens barrel b (56); the focusing mirror b (3), the optical filter b (5) and the diaphragm b (6) are sequentially arranged and have the same optical axis with the laser emitted by the ultraviolet xenon lamp light source b (1);
the second ultraviolet light source module (28) comprises an ultraviolet xenon lamp light source c (29), a focusing mirror c (27), an optical filter c (26) and a diaphragm c (25), wherein the ultraviolet xenon lamp light source c (29), the focusing mirror c (27), the optical filter c (26) and the diaphragm c (25) are packaged in a black lens barrel c (57); the ultraviolet xenon lamp light source c (29) is arranged at the bottom of the lens barrel c (57); the focusing mirror c (27), the optical filter c (26) and the diaphragm c (25) are sequentially arranged and have the same optical axis with the laser emitted by the ultraviolet xenon lamp light source c (29);
and the time sequence module is in signal connection with the visible laser diode, the ultraviolet xenon lamp light source b and the ultraviolet xenon lamp light source c.
6. The dual-wavelength multi-channel bioaerosol analyzer of claim 5, wherein the focusing mirror b (3) and the focusing mirror c (27) are made of ultraviolet transparent materials and coated with antireflection films.
7. The dual wavelength multi-channel bioaerosol analyzer of claim 1, wherein the first fluorescence detection channel (49) comprises a filter d (22), a focusing mirror d (23), a detector d (24); the optical filter d (22) is positioned in a reflection area of the dichroic mirror (18); the focusing lens d (23) is made of ultraviolet light-transmitting materials and is plated with an antireflection film, and the detector d (24) is positioned on the back focus of the focusing lens d (23); the optical filter d (22), the focusing mirror d (23) and the detector d (24) are sequentially arranged and coaxial;
the second fluorescence detection channel (50) comprises an optical filter e (19), a focusing mirror e (20) and a detector e (21), wherein the optical filter e (19), the focusing mirror e (20) and the detector e (21) are sequentially arranged and coaxial; the optical filter e (19) is positioned in the transmission area of the dichroic mirror (18), and the surface of the optical filter e is plated with an antireflection film; the detector e (21) is positioned on the back focal point of the focusing lens e (20);
the signal processing module processes and converts the voltage signals detected by the detector e (21) and the detector d (24).
8. The dual wavelength multi-channel bioaerosol analyzer of claim 7, wherein the detector e is a photomultiplier tube.
9. A dual wavelength multi-channel bioaerosol analyzer as defined in claim 1, wherein the gas circuit module comprises a sample introduction device (8), a filter (41), a gas pump (39), a first flow control valve (38), a second flow control valve (42), and a needle valve (52);
the sample feeding device (8) comprises a particle cutter (33), a sheath flow gas path (35), a sample flow gas pipe (36), a sheath flow nozzle (51) and a gas inlet (59); the particle cutter (33) is mounted to the sheath flow nozzle (51); the sheath flow nozzle (51) is hermetically arranged at the air inlet (59), and the outside of the sheath flow nozzle is made of stainless steel materials and is funnel-shaped; the sample gas flow pipe (36) is inserted into the center of the sheath flow nozzle (51) and coaxially arranged, the diameter of the sample gas flow pipe (36) is 0.7mm, the periphery of the sample gas flow pipe (36) is wrapped by sheath flow of a sheath flow gas path (35), and the diameter of the sheath flow gas path (35) is 1 mm;
the filter (41) is connected between the air outlet (40) of the detection cavity (10) and the air pump (39); the air outlet of the air pump (39) is divided into two paths: one path is connected with an exhaust port (37) of a sealed shell (53) through the first flow control valve (38), and the other path is connected with the sheath flow nozzle (51) through the second flow control valve (42); the needle valve (52) is connected between the sheath flow air outlet of the air pump and the detection cavity (10) through a thin air pipe.
10. A dual wavelength multi-channel bioaerosol analyzer as claimed in claim 9, wherein the filter element of the filter (41) has a filter pore size of 0.01 μm;
the flow rate of the air pump (39) is 3L/min.
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