CN214097163U - PCR all-in-one machine and optical detection device thereof - Google Patents
PCR all-in-one machine and optical detection device thereof Download PDFInfo
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- CN214097163U CN214097163U CN202023249098.4U CN202023249098U CN214097163U CN 214097163 U CN214097163 U CN 214097163U CN 202023249098 U CN202023249098 U CN 202023249098U CN 214097163 U CN214097163 U CN 214097163U
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Abstract
The utility model discloses a PCR all-in-one and optical detection device thereof. An optical detection device of a PCR all-in-one machine comprises an optical system, wherein the optical system comprises: a light source; a first lens group for processing light emitted from the light source into a first light beam; a dichroic mirror for reflecting the first light beam and allowing transmission of a second light beam from a sample; a second lens group for processing the first light beam reflected by the dichroic mirror to be irradiated onto a sample, and processing fluorescence from the sample into the second light beam; a fluorescence receiver for receiving the second light beam; and a third lens group for processing the second light beam transmitted by the dichroic mirror and providing the processed second light beam to the fluorescence receiver; the optical system further includes a filter disposed in the first lens group and/or the third lens group. The utility model provides high testing result's accuracy.
Description
Technical Field
The utility model belongs to the biological detection field relates to a PCR all-in-one and optical detection device thereof.
Background
The nucleic acid detection technology is a technology for directly detecting genetic materials of a living body, such as DNA and RNA, and has extremely high specificity and sensitivity, short window period and multiple detection capability. However, the process of nucleic acid detection is complicated, the number of steps is large, and the requirements on detection environment, laboratory conditions and technical level of personnel are high, so the development trend of nucleic acid detection is full-automatic integration, high integration, instant detection and random detection anywhere. In the field of in vitro diagnosis, such small, portable, rapid, simple, and Point-in-time detection means is called Point-of-care Test (POCT), and is also translated into Point-of-care Test (edge-of-bed Test), field Test (spot), and the like.
The PCR integrated machine is a detection instrument integrating nucleic acid extraction, amplification and detection aiming at nucleic acid detection. The optical detection device is an important component of the PCR integrated machine, and is mainly used for irradiating a nucleic acid amplification product, collecting a fluorescence signal generated by the nucleic acid amplification product and obtaining a qualitative or quantitative detection result according to fluorescence color or intensity and the like. The optical detection device has direct influence on the accuracy of the detection result of the PCR all-in-one machine.
SUMMERY OF THE UTILITY MODEL
To the technical problem, the utility model provides a PCR all-in-one and optical detection device thereof, it has improved the accuracy of testing result.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
an optical detection device of a PCR all-in-one machine comprises an optical system, wherein the optical system comprises:
a light source;
a first lens group for processing light emitted from the light source into a first light beam;
a dichroic mirror for reflecting the first light beam and allowing transmission of a second light beam from a sample;
a second lens group for processing the first light beam reflected by the dichroic mirror to be irradiated onto a sample, and processing fluorescence from the sample into the second light beam;
a fluorescence receiver for receiving the second light beam; and
a third lens group for processing the second light beam transmitted by the dichroic mirror and providing the processed second light beam to the fluorescence receiver;
the optical system further includes a filter disposed in the first lens group and/or the third lens group.
Preferably, the optical detection device further includes a housing, the optical system is disposed in the housing, and the housing is provided with a light inlet and a light outlet corresponding to the third lens group, a first connection hole corresponding to the light source, and a second connection hole corresponding to the fluorescence receiver.
More preferably, a first channel, a second channel and a third channel which are communicated with each other are formed in the housing, the light source and the first lens group are disposed in the first channel, the first connection hole is communicated with the second channel, the second lens group is disposed in the second channel, the light inlet/outlet port is communicated with the second channel, the fluorescence receiver and the third lens group are disposed in the third channel, the second connection hole is communicated with the third channel, and the dichroic mirror is disposed at a communication position of the three channels.
Furthermore, the center lines of the second channel and the third channel are coincident with each other, and the center line of the first channel is perpendicular to the center lines of the second channel and the third channel.
Preferably, the filter includes a first filter disposed in the first lens group and a second filter disposed in the third lens group.
More preferably, the first lens group includes at least two first lenses, one of the first lenses is used for collimating the light beam and the other of the first lenses is used for focusing the light beam, and the first filter is disposed between the two first lenses.
More preferably, the third lens group includes at least two third lenses, one of the third lenses is used for collimating the light beam and the other of the third lenses is used for focusing the light beam, and the second filter is disposed between the two third lenses.
Preferably, the optical system further includes an antireflection film provided in the second lens group.
More preferably, the second lens group includes at least two second lenses, one of the second lenses is used for collimating the light beam and the other of the second lenses is used for focusing the light beam, and the antireflection film is disposed between the two second lenses.
Preferably, the light source is an LED lamp.
The utility model discloses still adopt following technical scheme:
a PCR all-in-one machine comprises the optical detection device.
The utility model adopts the above scheme, compare prior art and have following advantage:
the utility model discloses a PCR all-in-one and optical detection device thereof, after handling the light that the light source sent, shine to the sample that awaits measuring again to fluorescent beam has been handled, has improved the accuracy of testing result greatly; the optical detection device has simple structure and is convenient to disassemble and assemble on the PCR all-in-one machine.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained without inventive work, wherein:
fig. 1 is a schematic perspective view of an optical detection apparatus according to an embodiment of the present invention.
FIG. 2 is a side view of the optical inspection device of FIG. 1;
fig. 3 is a sectional view taken along the line a-a in fig. 2.
Wherein:
1. a housing; 11. an optical inlet and outlet; 12. a first connection hole; 13. a second connection hole;
21. a light source; 22. a first lens; 23. a first optical filter; 24. a dichroic mirror; 25. a second lens; 26. an anti-reflection film; 27. a fluorescence receiver; 28. a third lens; 29. a second optical filter;
201. a first channel; 203. a second channel; 203. a third channel.
Detailed Description
The following detailed description of the preferred embodiments of the invention, taken in conjunction with the accompanying drawings, enables the advantages and features of the invention to be more readily understood by those skilled in the art. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. Furthermore, the technical features mentioned in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The terms "upper" and "lower" are defined herein based on the drawings and the orientation of the unitary apparatus in actual use.
The embodiment provides a PCR all-in-one machine and an optical detection device thereof. Referring to fig. 1 to 3, the optical detection device includes a housing 1 and an optical system disposed in the housing 1, and the optical detection device has a modular structure, can be used by being inserted into a designated position in a PCR integrated machine, and is very convenient to assemble.
As shown in fig. 2, the optical system includes a light source 21, a first lens group, a dichroic mirror 24, a second lens group, a fluorescence receiver (PD signal receiver) 27, and a third lens group. The light source 21 is for emitting detection light, and specifically, the light source 21 is an LED lamp. The first lens group is used for processing the light emitted by the light source 21 into a first light beam. Dichroic mirror 24 is used to reflect the first light beam and allow the second light beam from the sample to transmit. The second lens group is for processing the first light beam reflected by dichroic mirror 24 to be irradiated onto the sample, and processing the fluorescence from the sample into a second light beam. The fluorescence receiver 27 is for receiving the second light beam. The third lens group is used for processing the second light beam transmitted by dichroic mirror 24 and providing the processed second light beam to fluorescence receiver 27.
The optical system further includes a filter disposed in the first lens group and/or the third lens group. Specifically, the first lens group includes at least two first lenses 22, one of the first lenses 22 is used for collimating the light beam and the other of the first lenses 22 is used for focusing the light beam, and a first filter 23 is disposed between the two first lenses 22. The third lens group comprises at least two third lenses 28, wherein one third lens 28 is used for collimating the light beams, the other third lens 28 is used for focusing the light beams, and a second filter 29 is arranged between the two third lenses 28.
The second lens group comprises at least two second lenses 25, wherein one second lens 25 is used for collimating the light beam, the other second lens 25 is used for focusing the light beam, and an antireflection film 26 is arranged between the two second lenses 25.
In the optical system, an LED lamp is used as a point emission light source 21, the point emission light source is changed into parallel light through a first lens 22, the parallel light is converged through a second first lens 22 after the parallel light passes through a first optical filter 23, the light with a certain wavelength is reflected to the first and second lenses 25 through the dichroic mirror 24 (which is characterized in that the light with a certain wavelength almost completely penetrates through the dichroic mirror and the light with other wavelengths almost completely reflects), the light is dispersed to pass through the anti-reflection film 26, the light passes through the second and second lenses 25, the light with a required waveband is projected out and enters a reagent of the PCR all-in-one machine for fluorescence detection, the reflected light after detection similarly passes through the second and second lenses 25, the anti-reflection film 26 and the first and second lenses 25, passes through the dichroic mirror 24, and the light which passes through the dichroic mirror 24 passes through the two third lenses 28 and the second optical filter 29 and then enters the fluorescence receiver 27, and the signal of the light is processed, so that the detection result of the reagent is judged.
The housing 1 is formed with an optical inlet and outlet 11 corresponding to the third lens set, a first connecting hole 12 corresponding to the light source 21, and a second connecting hole 13 corresponding to the fluorescence receiver 27. The light inlet and outlet 11 faces the sample to be detected, so as to emit light beams to the sample to be detected and provide the fluorescence generated by the sample to enter the optical detection device. The first connection hole 12 is used for electrically connecting the light source 21 and a power supply of the PCR integrated machine to transmit power. The second connecting hole 13 is used for electrically connecting the fluorescent receiver 27 and a power supply and control device of the PCR all-in-one machine so as to transmit power and data.
Specifically, a first channel 201, a second channel 202 and a third channel 203 which are communicated with each other are formed in the housing 1, the light source 21 and the first lens group are arranged in the first channel 201, the first connection hole 12 is communicated with the second channel 202, the second lens group is arranged in the second channel 202, the light inlet/outlet 11 is communicated with the second channel 202, the fluorescence receiver 27 and the third lens group are arranged in the third channel 203, the second connection hole 13 is communicated with the third channel 203, and the dichroic mirror 24 is arranged at the communication position of the three channels. Further, the center lines of the second channel 202 and the third channel 203 coincide with each other, and the center line of the first channel 201 and the center lines of the second channel 202 and the third channel 203 are perpendicular to each other. That is, the second channel 202 and the third channel 203 are connected to form a straight channel, and the first channel 201 extends perpendicularly from the middle of the straight channel.
The optical detection device of the embodiment has the following advantages:
1. after being processed by a good light source and a dichroic mirror, a fluorescence receiver is arranged above the dichroic mirror and the fluorescence light beam is processed, so that the accuracy of a detection result is greatly improved;
2. the structure is used as a whole module, has simple structure and is convenient to disassemble and assemble on the PCR all-in-one machine.
The above embodiments are merely illustrative of the technical concepts and features of the present embodiments, and are a preferred embodiment, which is intended to enable a person skilled in the art to understand the contents of the present embodiments and implement the present embodiments, and not to limit the scope of the present embodiments. All equivalent changes and modifications made according to the present invention should be covered by the protection scope of the present embodiment.
Claims (10)
1. An optical detection device of a PCR all-in-one machine comprises an optical system, and is characterized in that the optical system comprises:
a light source;
a first lens group for processing light emitted from the light source into a first light beam;
a dichroic mirror for reflecting the first light beam and allowing transmission of a second light beam from a sample;
a second lens group for processing the first light beam reflected by the dichroic mirror to be irradiated onto a sample, and processing fluorescence from the sample into the second light beam;
a fluorescence receiver for receiving the second light beam; and
a third lens group for processing the second light beam transmitted by the dichroic mirror and providing the processed second light beam to the fluorescence receiver;
the optical system further includes a filter disposed in the first lens group and/or the third lens group.
2. The optical inspection device of claim 1, wherein: the optical detection device further comprises a shell, the optical system is arranged in the shell, and the shell is provided with a light inlet and a light outlet corresponding to the third lens group, a first connecting hole corresponding to the light source and a second connecting hole corresponding to the fluorescence receiver.
3. The optical detection device according to claim 2, characterized in that: the casing is provided with a first channel, a second channel and a third channel which are mutually communicated, the light source and the first lens set are arranged in the first channel, the first connecting hole is communicated with the second channel, the second lens set is arranged in the second channel, the light inlet and the light outlet are communicated with the second channel, the fluorescence receiver and the third lens set are arranged in the third channel, the second connecting hole is communicated with the third channel, and the dichroic mirror is arranged at the communication position of the three channels.
4. An optical inspection device according to claim 3, wherein: the center lines of the second channel and the third channel are overlapped, and the center line of the first channel is perpendicular to the center lines of the second channel and the third channel.
5. The optical inspection device of claim 1, wherein: the optical filter includes a first optical filter disposed in the first lens group and a second optical filter disposed in the third lens group.
6. The optical inspection device of claim 5, wherein: the first lens group comprises at least two first lenses, one first lens is used for collimating the light beam, the other first lens is used for focusing the light beam, and the first filter is arranged between the two first lenses.
7. The optical inspection device of claim 5, wherein: the third lens group comprises at least two third lenses, one third lens is used for collimating the light beams, the other third lens is used for focusing the light beams, and the second filter is arranged between the two third lenses.
8. The optical inspection device of claim 1, wherein: the optical system further includes an antireflection film disposed in the second lens group.
9. The optical inspection device of claim 8, wherein: the second lens group comprises at least two second lenses, one second lens is used for collimating the light beam, the other second lens is used for focusing the light beam, and the antireflection film is arranged between the two second lenses.
10. The utility model provides a PCR all-in-one which characterized in that: comprising an optical detection device according to any of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023249098.4U CN214097163U (en) | 2020-12-29 | 2020-12-29 | PCR all-in-one machine and optical detection device thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023249098.4U CN214097163U (en) | 2020-12-29 | 2020-12-29 | PCR all-in-one machine and optical detection device thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214097163U true CN214097163U (en) | 2021-08-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202023249098.4U Active CN214097163U (en) | 2020-12-29 | 2020-12-29 | PCR all-in-one machine and optical detection device thereof |
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| CN (1) | CN214097163U (en) |
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- 2020-12-29 CN CN202023249098.4U patent/CN214097163U/en active Active
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