CN114813665A - Multi-channel fluorescent quantitative detection device and molecular diagnosis platform - Google Patents
Multi-channel fluorescent quantitative detection device and molecular diagnosis platform Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 108
- 238000003745 diagnosis Methods 0.000 title abstract description 13
- 239000013307 optical fiber Substances 0.000 claims abstract description 111
- 230000003287 optical effect Effects 0.000 claims abstract description 87
- 238000001914 filtration Methods 0.000 claims abstract description 83
- 238000003384 imaging method Methods 0.000 claims abstract description 28
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 6
- 230000000903 blocking effect Effects 0.000 claims description 22
- 239000000741 silica gel Substances 0.000 claims description 8
- 229910002027 silica gel Inorganic materials 0.000 claims description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 6
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 2
- 229910052782 aluminium Inorganic materials 0.000 claims description 2
- 238000009413 insulation Methods 0.000 claims description 2
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- 238000004321 preservation Methods 0.000 description 9
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- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000001678 irradiating effect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
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- 230000003760 hair shine Effects 0.000 description 1
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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Abstract
The application relates to the technical field of fluorescence detection, in particular to a multi-channel fluorescence quantitative detection device and a molecular diagnosis platform. The fluorescence quantitative detection device comprises a light source component, a first light filtering component, a second light filtering component, an imaging component, an optical fiber bundle and a light detection groove. The optical detection groove is used for fixing a plurality of capillaries; the light source assembly can provide a white light source, and the white light source emitted by the light source assembly is irradiated by a plurality of capillaries corresponding to incident light fibers of a plurality of optical fiber bundles after incident light with a preset wave band is formed by filtering the incident light by the first filtering assembly so as to excite fluorescence. Fluorescence generated by the capillaries is filtered by the emergent optical fibers of the optical fiber bundles and the second filtering component to form fluorescence with corresponding wave bands and irradiates the imaging component, so that fluorescence imaging photos of the capillaries are conveniently and quickly obtained through the imaging component, the concentration of samples in the capillaries is analyzed, and the fluorescence quantitative detection efficiency of the samples is improved.
Description
Technical Field
The application relates to the technical field of fluorescence detection, in particular to a multi-channel fluorescence quantitative detection device and a molecular diagnosis platform.
Background
The fluorescence quantitative detection is a detection part in the last step of molecular diagnosis, in the detection process, excitation light with a preset wavelength is used for irradiating fluorescent substances in a capillary to excite fluorescence, and then the fluorescence is collected through a fluorescence collection device to realize the fluorescence quantitative detection of reactants.
Disclosure of Invention
The invention aims to provide a multi-channel fluorescence quantitative detection device and a molecular diagnosis platform, so as to improve the fluorescence detection efficiency to a certain extent.
The invention provides a multi-channel fluorescent quantitative detection device, which comprises a rack, a light source component, a first light filtering component, a second light filtering component, an imaging component, an optical fiber bundle and an optical detection groove, wherein the light source component is arranged on the rack;
the optical detection groove is arranged on the rack and used for fixing the capillary tube filled with the sample; the light source assembly and the first filtering assembly are arranged on the frame oppositely, the light source assembly is used for providing a white light source, and the white light source can penetrate through the first filtering assembly to form incident light with a preset waveband; the optical fiber bundle comprises three ports, a first port of the optical fiber bundle is connected with the first optical filtering component, a second port of the optical fiber bundle is connected with the optical detection groove, and the incident light can irradiate the capillary through an incident optical fiber between the first port and the second port so as to excite fluorescence; the second optical filter component and the imaging component are oppositely arranged on the frame, and a third port of the optical fiber bundle is connected with the second optical filter component, so that the fluorescence is irradiated to the second optical filter component through an emergent optical fiber between the second port and the third port; the second filtering component is used for filtering the fluorescence so as to enable the fluorescence with a preset wave band to irradiate the imaging component, and the imaging component is used for collecting the fluorescence and imaging;
the number of the capillaries is multiple, and the capillaries are arranged side by side at intervals; the number of the optical fiber bundles is multiple, the optical fiber bundles correspond to the capillaries one by one, and the second ports of the optical fiber bundles face the corresponding capillaries respectively.
Further, the first filtering component and the second filtering component respectively comprise a filtering wheel, a filter and a driving device; the driving device is fixedly arranged on the rack, and a driving end of the driving device is connected with the filter wheel so as to drive the filter wheel to rotate around the axis of the filter wheel; the number of the optical filters is multiple, and the optical filters are arranged at intervals along the circumferential direction of the optical filter wheel; the plurality of optical filters of the first optical filtering component are used for filtering the white light source to obtain incident light with different wave bands; the multiple filters of the second filter assembly are used for filtering the fluorescence so that the fluorescence with a preset wave band is irradiated into the imaging assembly.
Further, the first filtering component and the second filtering component respectively comprise light shields; the light blocking cover is covered on the light filtering wheel, the side walls of two sides of the light blocking cover are respectively provided with a light inlet and a light outlet, and the light inlet and the light outlet are positioned on the same axis; the filter wheel rotates to enable the plurality of optical filters to rotate between the light inlet and the light outlet in sequence, so that a light path channel is formed between the light inlet and the light outlet.
Further, the light source assembly comprises an LED lamp, a light source sleeve and a collimating lens; the light source sleeve is fixedly arranged on the rack and is coaxially arranged with the light inlet of the first light filtering component; one end of the light source sleeve extends into the light blocking cover through the light inlet of the first filtering component, the LED lamp is connected with the other end of the light source sleeve, and the collimating lens is installed in the light source sleeve.
Further, the light source assembly further comprises a heat sink; the radiating fin is arranged on the rack, one end of the light source sleeve, which is used for installing the LED lamp, is connected with the radiating fin, so that the LED lamp is clamped between the light source sleeve and the radiating fin, and the aluminum substrate of the LED lamp is attached to the radiating fin.
Furthermore, the multi-channel fluorescence quantitative detection device also comprises a first optical fiber lens component; the first optical fiber lens component comprises a first sleeve, a first focusing lens and a first optical fiber fixing piece; the first sleeve is fixedly arranged on the rack, and the first sleeve and the light outlet of the first light filtering component are coaxially arranged; one end of the first sleeve extends into a light blocking cover of the first light filtering component through a light outlet of the first light filtering component, and the other end of the first sleeve is connected with the first optical fiber fixing piece; the first port of the optical fiber bundle is inserted into the first sleeve through the first optical fiber fixing piece, the first focusing lens is installed in the first sleeve, and the first port of the optical fiber bundle is located on the focal plane of the first focusing lens.
Furthermore, the multi-channel fluorescence quantitative detection device also comprises a second optical fiber lens component; the second optical fiber lens component comprises a second sleeve, a second lens and a second optical fiber fixing piece; the second sleeve is fixedly arranged on the rack, and the second sleeve and a light inlet of the second light filtering component are coaxially arranged; one end of the second sleeve extends into the light blocking cover of the second light filtering component through the light inlet of the second light filtering component, and the other end of the second sleeve is connected with the second optical fiber fixing piece; the third port of the optical fiber bundle is inserted into the second sleeve through the second optical fiber fixing piece, and the second lens is arranged in the second sleeve; fluorescence emitted from the third port of the optical fiber bundle can be irradiated onto the optical filter of the second optical filter component located at the working position through the second lens.
Further, the imaging assembly includes a camera, a first lens, and a camera lens sleeve; the first lens is arranged in the camera lens sleeve, the camera lens sleeve is fixedly arranged on the rack and is coaxial with the light outlet of the second light filtering component, and one end of the camera lens sleeve extends into the light blocking cover of the second light filtering component through the light outlet of the second light filtering component; the camera is fixedly arranged on the frame, and the other end of the camera lens sleeve is connected with the camera so that the fluorescence passing through the second light filtering component irradiates on a CMOS chip of the camera.
Furthermore, the optical detection groove is formed with a plurality of detection chambers arranged side by side at intervals, and the plurality of detection chambers correspond to the plurality of capillaries one to one; a silica gel cover plate is arranged at the open end of the optical detection groove, and capillary tube inserting holes are formed in the positions, corresponding to the detection cavities, of the silica gel cover plate, so that the capillary tubes are inserted into the corresponding detection cavities; a plurality of light through holes are formed in the side wall of the light detection groove, and the light through holes are in one-to-one correspondence with and communicated with the detection chambers; an optical fiber fixing plate is arranged on the side wall of the optical detection groove, a plurality of optical fiber fixing holes are formed in the optical fiber fixing plate corresponding to the plurality of light through holes, and each optical fiber fixing hole and the corresponding light through hole are located on the same axis; a second focusing lens is respectively clamped between each light through hole and each corresponding light through hole; and the second ports of the optical fiber bundles are respectively inserted into the optical fiber fixing holes.
Further, a heating sheet is arranged on the bottom wall of the optical detection groove and used for heating the optical detection groove.
Furthermore, a first heat preservation chamber and a second heat preservation chamber are formed in the optical detection groove, and the detection chambers are located between the first heat preservation chamber and the second heat preservation chamber; and a heat insulation layer is arranged outside the optical detection groove to insulate the optical detection groove.
The invention also provides a molecular diagnosis platform which comprises the multichannel fluorescence quantitative detection device.
Compared with the prior art, the invention has the beneficial effects that:
the fluorescence quantitative detection device provided by the invention comprises a frame, a light source component, a first light filtering component, a second light filtering component, an imaging component, an optical fiber bundle and an optical detection groove. The optical detection groove is arranged on the rack and used for fixing the plurality of capillary tubes filled with the samples to be detected. The light source subassembly can provide white light source, and the light source subassembly sets up in the frame with first light filtering component relatively, and the white light source that the light source subassembly sent can shine on first light filtering component, then filters white light source through first light filtering component for the monochromatic light of predetermined wave band passes first light filtering component, forms the incident light that has predetermined wave band that is used for shining the capillary. The number of the optical fiber bundles is multiple, the optical fiber bundles correspond to the capillaries one by one, the first ports of the optical fiber bundles are converged into one strand to be connected into the first optical filter component, and incident light formed by the first optical filter component can uniformly enter the incident optical fibers through the first ports. The second ports of the optical fiber bundles are respectively connected into the optical detection grooves, and the second ports respectively face to the corresponding capillaries; so that the incident light is irradiated on the corresponding capillary tube via the second port, thereby exciting the fluorescent substance in the capillary tube to emit fluorescent light of a corresponding wavelength band. Second filtering component and formation of image subassembly set up in the frame relatively, and the third port of a plurality of fiber bundles assembles into one strand and inserts first filtering component for the fluorescence of the corresponding wave band that a plurality of capillaries sent can shine on second filtering component, and shines at the formation of image subassembly after the stray light is filtered to the second filtering component, thereby conveniently, swiftly obtains the fluorescence imaging photo of a plurality of capillaries through the formation of image subassembly, with the concentration of analysis sample, improves the fluorescence quantitative determination's of sample efficiency.
The invention also provides a molecular diagnosis platform which comprises the multi-channel fluorescence quantitative detection device, so that the molecular diagnosis platform also has the beneficial effect of the multi-channel fluorescence quantitative detection device.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic structural diagram of a multi-channel fluorescence quantitative detection apparatus provided in an embodiment of the present invention at a first viewing angle;
FIG. 2 is a schematic structural diagram of the multi-channel fluorescence quantitative detection apparatus provided in the embodiment of the present invention at a second viewing angle;
FIG. 3 is a cross-sectional view taken at A-A in FIG. 2;
FIG. 4 is a cross-sectional view taken at B-B of FIG. 2;
fig. 5 is a schematic structural diagram of an optical inspection groove according to an embodiment of the present invention.
Reference numerals:
1-a frame;
2-light source component, 21-LED lamp, 22-light source sleeve, 23-collimating lens, 24-radiating fin;
3-a first filter component, 31-a filter wheel, 32-a filter, 33-a driving device, 34-a light blocking cover, 35-a sensing piece and 36-a photoelectric sensor;
4-a second filter assembly;
5-imaging component, 51-camera, 52-camera lens sleeve, 53-first lens;
6-a first fiber lens assembly, 61-a first sleeve, 62-a first focusing lens, 63-a first fiber mount;
7-a second fiber lens assembly, 71-a second sleeve, 72-a second lens, 73-a second fiber mount;
8-optical detection groove, 81-light through hole, 82-optical fiber fixing plate, 83-optical fiber fixing hole and 84-silica gel cover plate.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention.
The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention.
All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The following describes a multi-channel fluorescence quantitative detection device and a molecular diagnosis platform according to some embodiments of the present application with reference to fig. 1 to 5.
The application provides a fluorescent quantitative detection device, which is used for carrying out fluorescent quantitative detection on a sample in a capillary; as shown in FIG. 1, the fluorescence quantitative detection device includes a frame 1, a light source assembly 2, a first filter assembly 3, a second filter assembly 4, an imaging assembly 5, a fiber bundle and a light detection tank 8.
Wherein the optical detection groove 8 is arranged on the frame 1, and the optical detection groove 8 is used for fixing the capillary tube filled with the sample to be detected. The number of the capillaries is plural, and the capillaries are arranged in the optical detection tank 8 side by side at intervals.
Light source subassembly 2 can provide the white light source, and light source subassembly 2 sets up on frame 1 with first light filter subassembly 3 relatively, and the white light source that light source subassembly 2 sent can shine on first light filter subassembly 3, then filters the white light source through first light filter subassembly 3 for the monochromatic light of predetermined wave band passes first light filter subassembly 3, forms the incident light that has predetermined wave band that is used for shining the capillary.
The optical fiber bundle is a three-port optical fiber bundle and comprises a first port, a second port and a third port, an incident optical fiber is formed between the first port and the second port, and an emergent optical fiber is formed between the second port and the third port. The number of the optical fiber bundles is multiple, the optical fiber bundles correspond to the capillaries one by one, the first ports of the optical fiber bundles are converged into one strand to be connected into the first optical filter component 3, and incident light formed by the first optical filter component 3 can uniformly enter the incident optical fibers through the first ports.
The second ports of the plurality of optical fiber bundles are respectively connected to the optical detection tank 8, and the plurality of second ports are respectively directed to the corresponding capillaries. The incident light is transmitted to the corresponding second ports through the plurality of incident optical fibers, and then the incident light of the predetermined wavelength band is irradiated on the corresponding capillaries through the second ports, so that the fluorescent substances in the capillaries are excited to emit fluorescent light of the corresponding wavelength band.
The second filtering component 4 is arranged on the frame 1, and the third ports of the plurality of optical fiber bundles are converged into one strand to be connected to the second filtering component 4, so that the fluorescent light with the corresponding wave band emitted by the plurality of capillaries can irradiate on the second filtering component 4; the imaging component 5 and the second filtering component 4 are arranged oppositely, fluorescence can irradiate the imaging component 5 through the second filtering component 4, stray light is removed through the second filtering component 4, and therefore fluorescence imaging photos of a plurality of capillaries can be conveniently and quickly obtained through the imaging component 5, the concentration of a sample can be analyzed, and the efficiency of fluorescence quantitative detection of the sample is improved.
In one embodiment of the present application, preferably, as shown in fig. 1 to 3, the first filter assembly 3 is capable of filtering the white light source emitted from the light source assembly 2 to form a plurality of monochromatic lights having different wavelength bands.
Preferably, as shown in fig. 3, the first filter assembly 3 includes a filter wheel 31, a filter 32, a driving device 33, and a light blocking cover 34; the driving device 33 is fixedly disposed on the frame 1, and the driving end of the driving device 33 is connected to the filter wheel 31 to drive the filter wheel 31 to rotate around the axis of the filter wheel 31 through the driving device 33. The number of the optical filters 32 is multiple, the optical filters 32 are distributed at intervals along the circumferential direction of the filter wheel 31, and the optical filters 32 can respectively pass through light rays with different wave bands. The light blocking cover 34 covers the filter wheel 31, a light inlet is formed on a side wall of the light blocking cover 34 opposite to the filter wheel 31, a light outlet is formed on a side wall of the light blocking cover 34 at the other side, and the light inlet and the light outlet are located on the same axis. The driving device 33 can drive the filter wheel 31 to rotate, so that the plurality of filters 32 sequentially rotate to between the light inlet and the light outlet and are located on the same axis with the light inlet and the light outlet, thereby forming a light path channel between the light inlet and the light outlet.
The white light source emitted from the light source assembly 2 can irradiate on the optical filter 32 between the light inlet and the light outlet through the light inlet, and then the white light source is filtered by the optical filter 32, so that the monochromatic light of the corresponding waveband passes through the optical filter 32 and is emitted through the light outlet to form the incident light with the predetermined waveband for irradiating the capillary.
In this embodiment, preferably, the structure of the second filter assembly 4 is the same as that of the first filter assembly 3, and is not described herein again; the number of the filters on the second filter assembly 4 is the same as that of the filters 32 on the first filter assembly 3, and the filters correspond to one another.
Preferably, the number of the filters 32 on the first filter assembly 3 is four, and the four filters 32 are a red filter, a yellow filter, a green filter and a blue filter respectively; the red filter is used for enabling red light at 607-644nm to pass through, the yellow filter is used for enabling yellow light at 552-594nm to pass through, the green filter is used for enabling green light at 509-545nm to pass through, and the blue filter is used for enabling blue light at 420-490nm to pass through.
When the incident light of the four wave bands irradiates on the capillary, the fluorescent substance in the capillary can excite the fluorescent light of the corresponding wave bands, namely red fluorescent light, yellow fluorescent light, green fluorescent light and blue fluorescent light. Correspondingly, the number of the optical filters on the second optical filter assembly 4 is also four, and the four optical filters are a red fluorescent optical filter, a yellow fluorescent optical filter, a green fluorescent optical filter and a blue fluorescent optical filter respectively; the red fluorescence filter is used for enabling 644-686nm red fluorescence to pass, the yellow fluorescence filter is used for enabling 594-634nm yellow fluorescence to pass, the green fluorescence filter 32 is used for enabling 545-583nm green fluorescence to pass, and the blue fluorescence filter is used for enabling 490-532nm blue fluorescence to pass.
In a specific using process, taking red incident light as an example, when the capillary tube needs to be irradiated by red incident light, the red filter and the red fluorescent filter are respectively rotated to the corresponding light path channels, so that the white light source emitted by the light source is filtered by the first filter component 3 to form 607-644nm incident light, and is covered on the capillary tube, and then the fluorescent substance in the capillary tube is excited to emit red fluorescent light; after the red fluorescence is filtered by the second filter assembly 4, 644-686nm red fluorescence is irradiated into the imaging assembly 5, and a fluorescence imaging photo formed by a plurality of capillaries under the excitation of 607-644nm incident light is obtained through the imaging assembly 5.
In one embodiment of the present application, it is preferable that the driving devices 33 of the first filter assembly 3 and the second filter assembly 4 are both stepping motors, so that the rotation angle of the corresponding filter wheel 31 can be precisely controlled by the stepping motors to rotate the desired filter 32 to the optical path channel.
Preferably, as shown in fig. 1, the first filter assembly 3 and the second filter assembly 4 are further provided with a photo sensor 36 at the stepping motor; one end of the output shaft of the stepping motor is connected with the filter wheel 31, and the other end of the output shaft is provided with the induction sheet 35, so that the induction sheet 35 can rotate synchronously at the same angle with the filter wheel 31.
The edge of the sensing piece 35 can extend into the photoelectric sensor 36, the edge of the sensing piece 35 is provided with a notch, and when the sensing piece 35 rotates to the position that the notch is positioned in the sensing area of the photoelectric sensor 36, the filter wheel 31 is at an initial position; then, with the initial position as a reference, the stepping motor drives the filter wheel to rotate by different angles, so that the four filters 32 can be sequentially rotated to the optical path channel.
In one embodiment of the present application, preferably, as shown in fig. 3, the light source assembly 2 includes an LED lamp 21, a light source sleeve 22, a collimating lens 23, and a heat sink 24. The light source sleeve 22 is fixedly arranged on the frame 1, and the light source sleeve 22 is positioned on one side of the first light filtering component 3, which is provided with a light inlet; the light source sleeve 22 is coaxially arranged with the light inlet of the first light filtering component 3, one end of the light source sleeve 22, which is far away from the first light filtering component 3, is connected with the LED lamp 21, and the other end of the light source sleeve 22 extends into the light blocking cover 34 through the light inlet of the first light filtering component 3 and extends to a position close to the light filtering wheel 31. The collimating lens 23 is arranged in the light source sleeve 22, the LED lamp 21 can emit white light, and the white light emitted by the LED is corrected by the collimating lens 23 to form a bundle of parallel light rays; the parallel light is irradiated onto the optical filter 32 located on the optical path channel, and then incident light of a predetermined wavelength band is formed by filtering of the optical filter 32 for exciting the fluorescent substance in the capillary tube.
In an embodiment of the present application, preferably, as shown in fig. 3, a first optical fiber lens assembly 6 is further disposed on one side of the first optical filter assembly 3 where the light outlet is formed; the first fiber optic lens assembly 6 includes a first sleeve 61, a first focusing lens 62 and a first fiber optic mount 63.
The first sleeve 61 is fixedly arranged on the frame 1, and the first sleeve 61 and the light outlet of the first light filtering component 3 are coaxially arranged; one end of the first sleeve 61 extends into the light blocking cover 34 of the first filtering component 3 through the light outlet of the first filtering component 3 and extends to the position of the filtering wheel 31, and the other end of the first sleeve 61 is connected with the first optical fiber fixing member 63; the first ports of the optical fiber bundles are converged into one strand and then are inserted into the first sleeve 61 through the first optical fiber fixing piece 63, the first focusing lens 62 is installed in the first sleeve 61, and the first ports of the optical fiber bundles are located on the focal plane of the first focusing lens 62; the incident light of the predetermined wavelength band obtained after being filtered by the first filter assembly 3 can be focused by the first focusing lens 62 and then uniformly dispersed into the first ports of the plurality of optical fiber bundles, and then transmitted to the corresponding second ports through the incident optical fibers of the plurality of optical fiber bundles, and then the incident light of the predetermined wavelength band is irradiated on the corresponding capillaries through the second ports, so that the fluorescent substances in the plurality of capillaries are excited to generate the fluorescence of the corresponding wavelength band.
In an embodiment of the present application, as shown in fig. 4, preferably, a second optical fiber lens assembly 7 is disposed on one side of the second optical filter assembly 4, where the light inlet is opened, and the second optical fiber lens assembly 7 includes a second sleeve 71, a second lens 72, and a second optical fiber fixing member 73. The second sleeve 71 is fixedly arranged on the frame 1, and the second sleeve 71 is coaxially arranged with the light inlet of the second filter assembly 4; one end of the second sleeve 71 extends into the light blocking cover of the second filter assembly 4 through the light inlet of the second filter assembly 4 and extends to the filter wheel of the second filter assembly 4, and the other end of the second sleeve 71 is connected to the second fiber fixing member 73. Third ports of the plurality of optical fiber bundles are converged into one strand and then are inserted into the second sleeve 71 through a second optical fiber fixing piece 73, and a second lens 72 is arranged in the second sleeve 71; the fluorescence emitted by the plurality of capillaries can enter the emergent optical fibers of the plurality of optical fiber bundles through the second ports of the corresponding optical fiber bundles, then is transmitted to the third ports of the plurality of optical fiber bundles through the plurality of emergent optical fibers, and irradiates the fluorescence emitted by the capillaries on the second lens 72 through the plurality of third ports; the second lens 72 is used for correcting the fluorescent light to obtain a parallel fluorescent light beam, and the parallel fluorescent light beam is irradiated on the filter of the second filter assembly 4 to filter the fluorescent light beam through the filter of the second filter assembly 4, so that the fluorescent light beam with a predetermined wavelength band is irradiated into the imaging assembly 5 to obtain a fluorescence imaging photo.
In an embodiment of the present application, preferably, as shown in fig. 1 and 4, the imaging assembly 5 is disposed on one side of the second filter assembly 4, where the light outlet is opened, the imaging assembly 5 includes a camera 51, a first lens 53 and a camera lens sleeve 52, the first lens 53 is mounted in the camera lens sleeve 52, and the first lens 53 and the camera lens sleeve 52 are disposed coaxially; the camera lens sleeve 52 is fixedly disposed on the frame 1, the camera lens sleeve 52 is disposed coaxially with the light exit of the second filter assembly 4, and one end of the camera lens sleeve 52 extends into the light blocking cover of the second filter assembly 4 through the light exit of the second filter assembly 4 and extends to the filter wheel. Preferably, the first lens 53 and the second lens 72 on both sides of the second filter assembly 4 are both achromatic double cemented lenses; the camera 51 is fixedly arranged on the frame 1, and the other end of the camera lens sleeve 52 is connected with the camera 51, so that the fluorescence emitted from the third port of the optical fiber bundle sequentially irradiates into the CMOS chip of the camera 51 after passing through the second lens 72, the second filter component 4 and the first lens 53, and thus, the fluorescence signal can be collected by the camera 51 without a camera lens and a fluorescence imaging photo can be obtained.
In one embodiment of the present application, preferably, as shown in fig. 5, the optical detection tank 8 is formed with a plurality of independent detection chambers, the plurality of detection chambers are arranged side by side at intervals, and the plurality of detection chambers and the plurality of capillaries correspond one to one. The upper end of the optical detection groove 8 is an open end, and the open end is covered with a silica gel cover plate 84 to seal a plurality of detection chambers through the silica gel cover plate 84. The silica gel cover plate 84 is provided with a capillary plugging hole corresponding to each detection chamber, so that a plurality of capillaries can be inserted into the corresponding detection chambers through the capillary plugging holes.
The side wall of one side of the optical detection groove 8 is provided with a light through hole 81 corresponding to each detection chamber, and the light through hole 81 is opposite to the pipe section at the end of the capillary tube, which is provided with the sample. The lateral wall of the side of the optical detection groove 8, which is provided with the light through hole 81, is provided with an optical fiber fixing plate 82, the optical fiber fixing plate 82 is provided with a plurality of optical fiber fixing holes 83, the optical fiber fixing holes 83 are in one-to-one correspondence with the light through holes 81, and the corresponding light through holes 81 and the optical fiber fixing holes 83 are located on the same axis. The second ports of the plurality of optical fiber bundles are respectively inserted into the plurality of optical fiber fixing holes 83, and a second focusing lens is arranged between each group of light passing holes 81 and the optical fiber fixing holes 83, so that incident light emitted from the second ports of the optical fiber bundles is focused by the corresponding second focusing lens and then irradiates the corresponding capillary tube, and fluorescent substances in the capillary tube are excited to emit fluorescence.
In this embodiment, it is preferable that a heating sheet is disposed on the bottom wall of the optical detection tank 8, and the optical detection tank 8 can be heated by the heating sheet, so that the detection chamber inside the optical detection tank 8 is at a suitable temperature, thereby ensuring the accuracy of the fluorescent quantitative detection.
Preferably, in order to further ensure that the plurality of detection chambers are at the same temperature, a first heat preservation chamber and a second heat preservation chamber are further formed in the optical inspection groove 8, the first heat preservation chamber is located at one end of the plurality of detection chambers, and the second heat preservation chamber is located at the other end of the plurality of detection chambers, so that the temperature of two detection chambers located at two ends of the plurality of detection chambers is prevented from being too low.
Preferably, the outside of light detection groove 8 still is provided with the heat preservation to further keep warm to a plurality of detection cavity, guarantee that the temperature in light detection groove 8 is in stable within range, provide fluorescence quantitative determination's accuracy.
The application also provides a molecular diagnosis platform, which comprises the multi-channel fluorescence quantitative detection device of any embodiment.
In this embodiment, the molecular diagnosis platform includes a multi-channel fluorescence quantitative detection device, so the molecular diagnosis platform has all the beneficial effects of the multi-channel fluorescence quantitative detection device, and is not described in detail herein.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and these modifications or substitutions do not depart from the spirit of the corresponding technical solutions of the embodiments of the present invention.
Claims (12)
1. A multi-channel fluorescence quantitative detection device is characterized by comprising a frame, a light source component, a first light filtering component, a second light filtering component, an imaging component, an optical fiber bundle and an optical detection groove;
the optical detection groove is arranged on the rack and used for fixing the capillary tube filled with the sample;
the light source assembly and the first filtering assembly are arranged on the frame oppositely, the light source assembly is used for providing a white light source, and the white light source can penetrate through the first filtering assembly to form incident light with a preset waveband;
the optical fiber bundle comprises three ports, a first port of the optical fiber bundle is connected with the first optical filtering component, a second port of the optical fiber bundle is connected with the optical detection groove, and the incident light can irradiate to the capillary through an incident optical fiber between the first port and the second port so as to excite fluorescence;
the second optical filter component and the imaging component are oppositely arranged on the frame, and a third port of the optical fiber bundle is connected with the second optical filter component, so that the fluorescence is irradiated to the second optical filter component through an emergent optical fiber between the second port and the third port;
the second filtering component is used for filtering the fluorescence so as to enable the fluorescence with a preset wave band to irradiate the imaging component, and the imaging component is used for collecting the fluorescence and imaging;
the number of the capillaries is multiple, and the capillaries are arranged side by side at intervals; the number of the optical fiber bundles is multiple, the optical fiber bundles correspond to the capillaries one by one, and the second ports of the optical fiber bundles face the corresponding capillaries respectively.
2. The multi-channel fluorescence quantitative detection device of claim 1, wherein the first filter assembly and the second filter assembly each comprise a filter wheel, a filter and a driving device;
the driving device is fixedly arranged on the rack, and a driving end of the driving device is connected with the filter wheel so as to drive the filter wheel to rotate around the axis of the filter wheel;
the number of the optical filters is multiple, and the optical filters are arranged at intervals along the circumferential direction of the optical filter wheel;
the plurality of optical filters of the first optical filtering component are used for filtering the white light source to obtain incident light with different wave bands;
the multiple filters of the second filter assembly are used for filtering the fluorescence so that the fluorescence with a preset wave band is irradiated into the imaging assembly.
3. The multi-channel fluorescence quantitative detection device of claim 2, wherein the first filter assembly and the second filter assembly further comprise a light blocking cover, respectively;
the light blocking cover is covered on the light filtering wheel, the side walls of two sides of the light blocking cover are respectively provided with a light inlet and a light outlet, and the light inlet and the light outlet are positioned on the same axis;
the filter wheel rotates to enable the plurality of optical filters to rotate between the light inlet and the light outlet in sequence, so that a light path channel is formed between the light inlet and the light outlet.
4. The multi-channel fluorescence quantitative detection device of claim 3, wherein the light source assembly comprises an LED lamp, a light source sleeve and a collimating lens;
the light source sleeve is fixedly arranged on the rack and is coaxially arranged with the light inlet of the first light filtering component;
one end of the light source sleeve extends into the light blocking cover through the light inlet of the first filtering component, the LED lamp is connected with the other end of the light source sleeve, and the collimating lens is installed in the light source sleeve.
5. The multi-channel fluorescence quantitative detection device according to claim 4, wherein the light source assembly further comprises a heat sink;
the radiating fin is arranged on the rack, one end of the light source sleeve, which is used for installing the LED lamp, is connected with the radiating fin, so that the LED lamp is clamped between the light source sleeve and the radiating fin, and the aluminum substrate of the LED lamp is attached to the radiating fin.
6. The multi-channel fluorescence quantitative detection device of claim 3, further comprising a first optical fiber lens assembly;
the first optical fiber lens component comprises a first sleeve, a first focusing lens and a first optical fiber fixing piece;
the first sleeve is fixedly arranged on the rack, and the first sleeve and the light outlet of the first light filtering component are coaxially arranged; one end of the first sleeve extends into the light blocking cover of the first light filtering component through the light outlet of the first light filtering component, and the other end of the first sleeve is connected with the first optical fiber fixing piece;
the first port of the optical fiber bundle is inserted into the first sleeve through the first optical fiber fixing piece, the first focusing lens is installed in the first sleeve, and the first port of the optical fiber bundle is located on the focal plane of the first focusing lens.
7. The multi-channel fluorescence quantitative detection device of claim 3, further comprising a second optical fiber lens assembly;
the second optical fiber lens component comprises a second sleeve, a second lens and a second optical fiber fixing piece;
the second sleeve is fixedly arranged on the rack, and the second sleeve and a light inlet of the second light filtering component are coaxially arranged; one end of the second sleeve extends into the light blocking cover of the second light filtering component through the light inlet of the second light filtering component, and the other end of the second sleeve is connected with the second optical fiber fixing piece;
the third port of the optical fiber bundle is inserted into the second sleeve through the second optical fiber fixing piece, and the second lens is arranged in the second sleeve;
fluorescence emitted from the third port of the optical fiber bundle can be irradiated onto the optical filter of the second optical filter component located at the working position through the second lens.
8. The multi-channel quantitative fluorescence detection device of claim 3, wherein the imaging assembly comprises a camera, a first lens and a camera lens sleeve;
the first lens is arranged in the camera lens sleeve, the camera lens sleeve is fixedly arranged on the rack and is coaxial with the light outlet of the second light filtering component, and one end of the camera lens sleeve extends into the light blocking cover of the second light filtering component through the light outlet of the second light filtering component;
the camera is fixedly arranged on the frame, and the other end of the camera lens sleeve is connected with the camera so that the fluorescence passing through the second light filtering component irradiates on a CMOS chip of the camera.
9. The multi-channel quantitative fluorescence detection device of claim 1, wherein the optical detection groove is formed with a plurality of detection chambers arranged side by side at intervals, and the plurality of detection chambers correspond to the plurality of capillaries one to one;
a silica gel cover plate is arranged at the open end of the optical detection groove, and capillary tube inserting holes are formed in the positions, corresponding to the detection cavities, of the silica gel cover plate, so that the capillary tubes are inserted into the corresponding detection cavities;
a plurality of light through holes are formed in the side wall of the light detection groove, and the light through holes are in one-to-one correspondence with and communicated with the detection chambers;
an optical fiber fixing plate is arranged on the side wall of the optical detection groove, a plurality of optical fiber fixing holes are formed in the optical fiber fixing plate corresponding to the plurality of light through holes, and each optical fiber fixing hole and the corresponding light through hole are located on the same axis;
a second focusing lens is respectively clamped between each light through hole and each corresponding light through hole; and the second ports of the optical fiber bundles are respectively inserted into the optical fiber fixing holes.
10. The multi-channel fluorescent quantitative detection device of claim 9, wherein a heating plate is disposed on the bottom wall of the optical detection tank for heating the optical detection tank.
11. The multi-channel quantitative fluorescence detection device of claim 9, wherein the optical detection tank further forms a first incubation chamber and a second incubation chamber, and the plurality of detection chambers are located between the first incubation chamber and the second incubation chamber;
and a heat insulation layer is arranged outside the optical detection groove to insulate the optical detection groove.
12. A molecular diagnostic platform comprising the multi-channel fluorescence quantitative detection device of any one of claims 1 to 9.
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| CN202110129513.2A CN114813665A (en) | 2021-01-29 | 2021-01-29 | Multi-channel fluorescent quantitative detection device and molecular diagnosis platform |
| PCT/CN2021/117496 WO2022160726A1 (en) | 2021-01-29 | 2021-09-09 | Multi-channel fluorescence quantitative detection device and molecular diagnosis platform |
| PCT/CN2021/138615 WO2022160998A1 (en) | 2021-01-29 | 2021-12-16 | Molecular diagnostic platform |
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| CN104614351A (en) * | 2015-01-21 | 2015-05-13 | 南京中科神光科技有限公司 | Rapid and multi-channel real-time fluorescence quantification detection device |
| CN108351303A (en) * | 2015-11-23 | 2018-07-31 | 珀金埃尔默健康科学公司 | Hollow polymer fiber optic system for single analyte and multiplexed analyte detection |
| WO2019244358A1 (en) * | 2018-06-22 | 2019-12-26 | 株式会社日立ハイテクノロジーズ | Electrophoresis apparatus |
| CN215066164U (en) * | 2021-01-29 | 2021-12-07 | 广东润鹏生物技术有限公司 | Multi-channel fluorescent quantitative detection device and molecular diagnosis platform |
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| KR100647317B1 (en) * | 2005-02-03 | 2006-11-23 | 삼성전자주식회사 | Optical system for multi-channel fluorescence measurement of microfluidic chip and multi-channel fluorescence sample analyzer |
| CN206594050U (en) * | 2017-03-09 | 2017-10-27 | 无锡中德伯尔生物技术有限公司 | A kind of food safety detection module |
| CN209065925U (en) * | 2018-08-31 | 2019-07-05 | 北京乐普医疗科技有限责任公司 | A kind of multichannel fluorescence quantitative PCR instrument |
| CN111830002A (en) * | 2020-08-10 | 2020-10-27 | 杭州天微基因科技有限公司 | Multi-channel detection system for real-time nucleic acid fluorescence detection |
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- 2021-09-09 WO PCT/CN2021/117496 patent/WO2022160726A1/en active Application Filing
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104614351A (en) * | 2015-01-21 | 2015-05-13 | 南京中科神光科技有限公司 | Rapid and multi-channel real-time fluorescence quantification detection device |
| CN108351303A (en) * | 2015-11-23 | 2018-07-31 | 珀金埃尔默健康科学公司 | Hollow polymer fiber optic system for single analyte and multiplexed analyte detection |
| WO2019244358A1 (en) * | 2018-06-22 | 2019-12-26 | 株式会社日立ハイテクノロジーズ | Electrophoresis apparatus |
| CN215066164U (en) * | 2021-01-29 | 2021-12-07 | 广东润鹏生物技术有限公司 | Multi-channel fluorescent quantitative detection device and molecular diagnosis platform |
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