CN113528675A - Molecular marker for identifying duck slaughter traits based on myostatin gene MSTN, and identification method and application thereof - Google Patents
Molecular marker for identifying duck slaughter traits based on myostatin gene MSTN, and identification method and application thereof Download PDFInfo
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Abstract
The invention discloses a molecular marker for identifying duck slaughter traits based on myostatin gene MSTN, and an identification method and application thereof, wherein the molecular marker is T or C, the molecular marker is located at 458 th position of the myostatin gene MSTN, and the myostatin gene MSTN has a nucleotide sequence shown as SEQ ID No. 1. According to the invention, the polymorphism of the 458 th base on the CDS sequence exon1 of the MSTN gene is utilized to select the duck slaughtering traits according to the genotype. The method for selecting the slaughter traits of the ducks has the advantages of early selection, time saving, simplicity in operation, cost saving, high accuracy and the like.
Description
Technical Field
The invention relates to the technical field of molecular markers, in particular to a molecular marker for identifying duck slaughter traits based on myostatin gene MSTN, and an identification method and application thereof.
Background
The duck meat is an important high-quality protein source for Chinese consumers, has unique flavor, is rich in unsaturated fatty acid beneficial to human health, accounts for about 25% of the consumption of poultry, and is popular with consumers. The meat ducks mainly comprise white feather meat ducks, meat ducks with numb feather, muscovy ducks and the like, wherein the slaughtering amount of the white feather meat ducks accounts for 80% of the slaughtering amount of the whole meat ducks. At present, the main varieties of the white feather meat ducks fed by the feed comprise cherry valley meat ducks, Z-type Beijing ducks and the like. The Q strain white feather meat duck is a newly bred strain, breeding of 6 generations is continuously carried out, and the growth and development rules and the like of the white feather meat duck are lack of research. The MSTN gene is used as a candidate gene influencing slaughter traits, SNPs sites of the meat ducks are screened, the influence of the SNPs sites on the slaughter traits is analyzed, and reference is provided for continuous breeding, molecular marker assisted selection and feeding management of Q-line white feather meat ducks.
The MSTN gene was found in skeletal muscle of mice by researchers of McPhellton et al in 1997 and has a significant inhibitory effect on both hyperplasia and hypertrophy of muscle, so it was named myostatin (Bogdaovich et al, 2010; McPherron et al, 1997). The MSTN gene is mainly expressed in the secretory protein of muscle tissue, and its main function is to regulate the growth and differentiation of muscle cells, playing a key role in the growth and development of mammals and aquatic organisms. After birth, the MSTN gene negatively regulates skeletal muscle growth, primarily by inhibiting skeletal muscle growth (Mcpherron et al, 1997). The deletion or activity reduction of the MSTN gene brings good news to the improvement of the meat performance of livestock and poultry and the improvement of the economic benefit of cultivation. Kambadur found that the gene was mutated in a double-muscle cow, which provided 1.3-fold more meat product than regular cattle under the same feeding conditions (Kambadur et al, 1997). The MSTN gene mutation is also found in poultry, and Xu et al (2013) find that 3 single nucleotide mutant SNPs exist in the gene through research on Beijing ducks, wherein T129C is obviously related to the breast muscle thickness, T952C is obviously related to the keel length, the relation between the MSTN gene polymorphism and the Beijing duck breast muscle character is disclosed (Xu et al.,2013), and the gene polymorphism is disclosed to be related to poultry carcass performance.
Based on the above, in order to accurately select a genotype more suitable for growth and development and further provide data for early breeding, and simultaneously to save production cost and accelerate genetic progress, a molecular marker for identifying duck slaughter traits based on myostatin gene MSTN, and an identification method and application thereof are provided.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, provides a molecular marker which can identify duck slaughter traits based on myostatin gene MSTN after overexpression, and an identification method and application thereof, and aims at the SNP (single nucleotide polymorphism) molecular marker of candidate genes related to the duck slaughter traits so as to solve the problems of slow progress of conventional phenotypic breeding and early identification of the slaughter traits.
The invention realizes the purpose through the following technical scheme:
the invention provides a molecular marker for identifying duck slaughter traits based on a myostatin gene MSTN, wherein the molecular marker is T or C and is positioned at 458 th position of the myostatin gene MSTN.
The further improvement is that the MSTN has a nucleotide sequence shown as SEQ ID NO. 1.
The invention also provides application of the molecular marker for identifying the duck slaughter traits based on the myostatin gene MSTN in identifying the meat duck slaughter traits.
The invention also provides an identification method for identifying duck slaughter traits by using the molecular marker, which comprises the following steps:
(1) extracting the total DNA of vein blood of the wings of the meat ducks;
(2) designing a specific amplification primer by taking the site where the molecular marker is located and a sequence consisting of upstream and downstream bases as a target sequence, and carrying out PCR amplification by using the specific amplification primer by taking the total DNA as a template to obtain an amplification product;
(3) carrying out genotyping detection and sequencing on the amplification product to obtain the molecular marker type of the meat duck to be detected;
(4) and judging the slaughtering traits of the meat ducks according to the type of the molecular marker.
The further improvement is that the target sequence is shown as SEQ ID NO. 2.
In a further improvement, the specific amplification primer sequence is:
SEQ ID NO.3：F1：TAAGGTCCGCCTTGACCTGT
SEQ ID NO.4：R1：CGGGACATTTTCATCCAGTGC。
the further improvement is that the genotyping detection method comprises the steps of carrying out non-denaturing polyacrylamide gel electrophoresis and silver staining on a PCR amplification product to obtain an image, and carrying out genotyping according to the image:
(1) only contains 1 band, and is CC type;
(2) contains 2 thin strips, and is TT type;
(3) containing 1 coarse band and 1 fine band, is TC type.
The further improvement is that the specific steps for judging the slaughtering traits of the meat ducks according to the molecular marker types in the step (4) are as follows:
(1) if the molecular marker type of the meat duck to be detected is TT type, the slaughtering character of the meat duck is the best;
(2) if the molecular marker type of the meat duck to be detected is CC type, the slaughtering character of the meat duck is the worst;
(3) if the molecular marker type of the meat duck to be detected is TC type, the slaughter traits of the meat duck are moderate.
The invention has the beneficial effects that: the invention provides a molecular marker which can identify duck slaughter traits based on myostatin gene MSTN after overexpression, and an identification method and application thereof, wherein the duck slaughter traits are selected according to genotypes by utilizing polymorphism of 458 th base on CDS sequence exon1 of the MSTN gene. The method for selecting the slaughter traits of the ducks has the advantages of early selection, time saving, simplicity in operation, cost saving, high accuracy and the like.
Drawings
FIG. 1 shows the result of electrophoresis detection of DNA extracted from duck genome by using the kit method in the embodiment of the present invention.
FIG. 2 is an electrophoretogram of PCR-specific amplification products of a portion of samples in an example of the present invention; where M is a 1kb DNA Marker, and lanes 1-12 are bands of specific amplification products.
FIG. 3 is a non-denaturing polyacrylamide gel electrophoresis of a portion of the PCR-specific amplification products from a sample of an example of the invention.
FIG. 4 shows the result of the locus 458-site genotype-based sequencing verification in the exon of the MSTN gene.
Detailed Description
The present application will now be described in further detail with reference to the drawings, it should be noted that the following detailed description is given for illustrative purposes only and is not to be construed as limiting the scope of the present application, as those skilled in the art will be able to make numerous insubstantial modifications and adaptations to the present application based on the above disclosure.
1. Material
The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used therein are commercially available products unless otherwise specified.
2. Method of producing a composite material
2.1 obtaining duck MSTN gene polymorphic site
2.1.1 genomic DNA extraction and detection
400Q-series white feather meat ducks are selected as test materials, Blood sampling is carried out on the duck wing veins at the age of 6 weeks, and Blood genome DNA extraction Kit (TLANamp Blood DNA Kit) is used for extracting Blood DNA, and the specific reference to the Kit is carried out according to the use instruction.
The DNA concentration and OD value were measured using a NanoDrop 2000. The DNA was detected by 1.5% agarose gel electrophoresis, and the results are shown in FIG. 1, the extracted genomic DNA was of good quality, single and clear main band.
2.1.2 primer design
Finding a DNA sequence corresponding to the myostatin gene MSTN shown by SEQ ID NO.1 from a duck genome database, using a partial nucleotide sequence of the myostatin gene MSTN shown by SEQ ID NO.1 as a template, designing and checking a specific amplification Primer by using Primer Premier 6.0 and Oligo7 software, and paying attention to the fact that an SNP site is arranged at a middle position as much as possible in the process of Primer design to avoid the occurrence of conditions such as hairpin structures, Primer dimers, mismatches and the like, so that the Primer sequence is optimized, wherein the Primer sequence is shown as follows:
F1：TAAGGTCCGCCTTGACCTGT(SEQ ID NO.3)
R1：CGGGACATTTTCATCCAGTGC(SEQ ID NO.4)
the amplifiable region of the primer has a 339bp sequence, and the primer comprises a 458 th molecular marker.
2.1.3 PCR amplification
Carrying out PCR amplification reaction on a target fragment of the MSTN gene by using the synthesized sequencing specific primer, wherein the PCR amplification system is as follows:
the PCR reaction conditions are as follows: pre-denaturation at 95 ℃ for 5 min; first, denaturation at 95 ℃ for 45 s; a second step of annealing at 62 ℃ for 45s (the annealing temperature depends on the primer); a third step of extension at 72 ℃ for 30s, wherein the second step to the third step are cycled for 31 times, and 32 cycles are total; extension at 72 ℃ for 10 min.
2.1.4 detection of PCR amplification products
Preparing 1.5% agarose gel, performing electrophoresis detection at 100V for 40min, and obtaining a band with a length slightly larger than 300bp as shown in FIG. 2, which is consistent with the predicted length, indicating that the target fragment is obtained. And (3) recovering a PCR amplification product by using a DNA recovery kit of Dalibao biology, Inc., and sending the obtained PCR product to Shanghai to carry out sequencing, wherein the sequence is shown as SEQ ID NO 2 and is consistent with a prediction result.
2.1.5 genotyping assay
The PCR product was subjected to non-denaturing polyacrylamide gel electrophoresis and silver staining to obtain images, and the results are shown in FIG. 3. Screening out different genes according to the result of the photographed image:
(1) only contains 1 band, and is CC type;
(2) contains 2 thin strips, and is TT type;
(3) containing 1 coarse band and 1 fine band, is TC type.
2.1.6 DNA proof sequencing
According to the typing result, typing and sorting different results of different individual glue running pictures, selecting individuals with different forms for repeated PCR amplification, sending the individuals to Shanghai Bioengineering technology service Limited for bidirectional sequencing verification, analyzing the sequencing result by using ChromasPro software, verifying SNP sites, and obtaining the result shown in figure 4, which is the molecular marker related to the invention.
2.2 correlation analysis of mutation site of MSTN gene C458T and slaughter trait of Q-series white feather meat duck
2.1.1 genotyping
In order to determine the relevance of the C/T polymorphism of 458 th base (C458T) of exon1 of Q-series white feather meat duck MSTN gene and important phenotypic characters of the meat ducks, 400Q-series white feather meat ducks in 2.1.1 are used as test materials, and meat ducks are slaughtered at the age of 6 weeks to determine important economic characters of live weight, carcass weight, breast muscle weight, leg muscle weight, abdominal fat weight, semi-bore weight, full-bore weight and slaughter rate, and semi-bore rate, full-bore rate, breast muscle rate, leg muscle rate, lean meat rate and abdominal fat rate.
400Q-line white-feather meat ducks were genotyped by the genotyping method of 2.1.5, and the results are shown in Table 1.
TABLE 1 results of genotype measurements for individuals with different phenotypes
The chi-square test result shows that the genotype distribution of the test duck group is in Hardy-Weinberg balance.
2.1.2 statistical analysis
The association between the three genotypes and the slaughter performance was analyzed by one-way ANOVA in SPSS20.0, and the results of the association analysis between the different genotypes and the various traits are shown in table 2:
TABLE 2 correlation analysis of MSTN genotype and slaughtering performance of white feather meat ducks
Note: the upper right hand box with different lower case letters indicates significant difference (P < 0.05).
As can be seen from Table 2, in the meat ducks of the three genotypes of CC, CT and TT, the TT type is obviously higher than the CC type (P is less than 0.05) meat ducks in live weight, carcass weight, half-bore weight and full-bore weight, and the difference of other indexes among the genotypes is not obvious (P is more than 0.05). In conclusion, it can be concluded that the carcass traits are better in TT type individuals.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Sequence listing
<110> agriculture university of Anhui
Huangshan Qiangying Duck industry Co Ltd
<120> molecular marker for identifying duck slaughter traits based on myostatin gene MSTN, and identification method and application thereof
<141> 2021-07-22
<160> 4
<170> SIPOSequenceListing 1.0
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<211> 5640
<212> DNA
<213> Duck (DUCK)
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atataaggta caccagtgtg gcaagctgtc tctcagattg catttgcttt cacggatcta 60
tttagtacta aaagaaaaag ggaaagggga agggaaaaaa aaaaagggaa aaaagctgca 120
ctgaatgtga gatcatgcaa aagctagcaa tctatgttta tatttacctg ttcatgctga 180
tttcagttga tccggtggct cttgatgacg gcagtcagcc cacagagaac gctgaaaaag 240
atggactgtg caatgcttgt acatggagac agaatacaaa atcttccaga atagaagcca 300
taaaaattca aatcctcagc aaactgcgcc tggaacaagc tcctaacatt agcagggatg 360
ttattaagca acttttaccc aaagctcctc cactacagga actgattgat caatatgacg 420
tccagagaga cgacagtagc gatggctctt tggaagatga tgactatcat gccacaactg 480
aaacgattat cacaatgcct acagagtgta agtaaccctg ctgctttcgt ctcgcaccgc 540
tcctccgaga gagttgtctc cgtgctgtag agccacacaa ctcctgctca ggctctccca 600
acaggctgct ggctggagtc tgccggaggg agggaggaga gtgtatttct aaaggatttg 660
agtcaggttc aaatgacctt gttgcgagcc tttgttttac tgttcattta ttcggctcct 720
tataacgtgt gtgccctgta gcacttacga tttgccaatt gccttagaaa gcaaggagag 780
atctgcagtt cagttggctc cgttatttgc agcattcagg gcatttccta ttgcaaacag 840
aaagtgcaag tgcaggtaac aagatagcta tatttttctg cgtgagcttt caccagccag 900
tgctttaaag agcaacagaa atgagcagaa atacagctta gagaacgaga cccacaagtg 960
caagtataga gttaaattct tgcttctttg ctgatcccat aagctagcac cacccacctc 1020
cgagttaaaa ctatatattt aaagcaaata tgtgtaaacc gatttaaatg ttaaaatgtc 1080
cacgttatag caatcagctc cagtagtcgt gccaaggaag aatgaaggca tttactgaat 1140
gagcagattc taagttatct tctgagtgct aaacatctgc tacggctcct atcaagtatc 1200
ttttgcacag ctaatactat agtatcttat gttcttcatc agctaaagta tttggctatg 1260
gcagctgttt tttaaaataa cacttatgaa tatgatgtca aaaaggaaaa agatgacagg 1320
acatattagg catgttagca gttacagata tcattacaat acacatacta gaataaaatc 1380
agctgctgta gccagctaaa atgcacacat gggtgaaaaa ttaactttat ctgtaacatt 1440
ttttaacttt aataatatgt gtatgtaata gcaacatatt agtgcagcct taatgcaaaa 1500
ttgaggtctg ccagcagttt tattctagcc tgtatatatt tgggaactat tttaattaaa 1560
ccactacgta attacaatga ctgaagaaaa gtcattatgc ttcactgatc tgagttaatt 1620
tcctactttt tataaaagcc tttgaactat ttaaaatcca ctaaagatac aggtgtctta 1680
agtgatgaga tttatgagtg tcaaaaccct gcaccattta aaattcattt tacccatttt 1740
atgctggcat ggcaagacac tgtcatgact aggtagaaca gctgcatcgt aggctacagg 1800
aaggaggaat gctgcactgc atgtgacttt cctataatat tgttcaaatg gaaacaaatc 1860
tttacattca tactttaaat tattattgtt tccactcagc aaaagaaaag gaagctttca 1920
ggtagtttgt gcacagctac ttctgtttgt atgatgcaca gcacactaag gttatctagg 1980
aaattaaaaa tacctaaagg tgacgtaagt gttgactcag ctgatgcaaa gttgctattg 2040
tgcatgtttt atggagctcc tcaggagatg aacagcagaa atgagctggt agcagtccct 2100
gtgaagtggc ataggctgtg ccctatcccc tgctcacttt tatctgggaa aggagctttt 2160
ctgcagctgt aggggtgagc ccacggtgct ggtacaactg gtcagatcct agagataacg 2220
gaggaccaca gagtttctct acagcgccct tcttctcaca gttgcccatc actcctgaac 2280
tgcagcctgt gcacatcctt cactctgctt agtctacaga gtgcaaggga ccagctagag 2340
ttacacataa aagggttagc caaatctcct tttagcctat ttacaaaaca gtcggtgttt 2400
ccttgtcacn nnnnnnnnng ggaactattt taattaaacc actacgtaat tacaatgact 2460
gaagaaaagt cattatgctt cactgatctg agttaatttc ctacttttta taaaagcctt 2520
tgaactattt aaaatccact aaagatacag gtgtcttaag tgatgagatt tatgagtgtc 2580
aaaaccctgc accatttaaa attcatttta cccattttat gctggcatgg caagacactg 2640
tcatgactag gtagaacagc tgcatcgtag gctacaggaa ggaggaatgc tgcactgcat 2700
gtgactttcc tataatattg ttcaaatgga aacaaatctt tacattcata ctttaaatta 2760
ttattgtttc cactcagcaa aagaaaagga agctttcagg tagtttgtgc acagctactt 2820
ctgtttgtat gatgcacagc acagcacact aaggttatct aggaaattaa aaatacctaa 2880
aggtgacgta agtgttgact cagctgatgc aaagttgcta ttgtgcatgt tttatggagc 2940
tcctcaggag atgaacagca gaaatgagct ggtagcagtc cctgtgaagt ggcataggct 3000
gtgccctatc ccctgctcac ttttatctgg gaaaggagct tttctgcagc tgtaggggtg 3060
agcccacggt gctggtacaa ctggtcagat cctagagata acggaggacc acagagtttc 3120
tctacagcgc ccttcttctc acagttgccc atcactcctg aactgcagcc tgtgcacatc 3180
cttcactctg cttagtctac agagtgcaag ggaccagcta gagttacaca taaaagggtt 3240
agccaaatct ccttttagcc tatttacaaa acagtcggtg tttccttgtc acatgatgct 3300
acttttaggt gacagcaaaa tgattaaatt tgccgtagag cgtaaagcac agaaaatgaa 3360
tgatgttaca tccacttgtt agtgatgctg tttgtagaat attgtaagac atcctacatg 3420
gtctggaaaa aaaattgggt ttatatatgc atatttcttt ttgttccctg ttcagtaatc 3480
tattctttcc attcatttat agctgatttt cttgtacaaa tggagggaaa accaaaatgt 3540
tgcttcttta agtttagctc taaaatacaa tataacaaag tagtaaaggc acaattgtgg 3600
atatacttga ggcaagtcca aaaacctaca acagtgtttg tgcagatcct gagacttatt 3660
aagcccatga aagacggtac aagatatact ggaattcgat ctttgaaact tgacatgaac 3720
ccaggcactg gtatttggca gagtattgat gtgaagacag tgttgcaaaa ttggctcaaa 3780
cagcctgaat ccaatttagg catcgaaata aaagcttttg atgagaatgg acgagatctt 3840
gctgtaactt tcccaggacc aggtgaagat ggattggtaa gtttatttag aaaaatcccg 3900
tttaaatatc ttgcatttta ttggaaagca tttaacttgt gttttaagga taaaatggaa 3960
ccgttgttta gggaatggaa gaaaaaggct ctgtatgccc aattctttcg ctctttgctc 4020
tctgatcatg gcaaaaatca cgtagcactc ctgtgcctcc atttccctat ttgaaaaatt 4080
gaggtgaatt gcattacctc agggagatgt tgagaggcat caactggaaa atgttttggt 4140
acgtaccaat ggatggtact atcatagtaa cactgtgttc aaatttgaga gcaaacactg 4200
cttaatgaga agtgactttg cataaaggta aaagagtcat ttgattattt cattgatggg 4260
aattttgcct cagatactgc tgaaaagatt ttgcattcaa accctgtttc tccctattct 4320
gtatgcatgt attttctttt aatgataaga tgaattatct gtatttatga aggtgacaac 4380
agatgtcata attttaaatg ttgtacatat gatagaatgc gtcattcctc ttattagaaa 4440
tgtttgaata gataaattct acttgataca ataaatgggt cacaatgatg ggaaagagca 4500
ttaaataact atatagtttc tctctgtaag tatggatgaa tgaattaaat ctgaagcgta 4560
ccagctaaac tacagcattt tccttgctgt ttccccaaat cactcatcat ctacaacctg 4620
acaaaattcc tgaacctatt gcaatagcta aaacaatcac aaccaaaacg tttctgcttt 4680
tgagtaaagt accaggagca ttattttgct ttcactgcta cagagaagat cagtctgttt 4740
aggtagctat gtaatgttta acttcatagc agtgcaatgc agttaacaga acctagagta 4800
aaaacaatgt ggtatcccaa tcacagattc attacagatg tggctctcat ctgcttgctg 4860
gatatcatga taaagaatgt ctccacccat tcaaatatgt aacaaggtgt tttcacacgt 4920
gtggataacc cttggttttc tcagggttat catcagctgt aaaaacgaac tgctgtaaaa 4980
gcaacctggg gtcagatgca ctgcaggtaa tgggagagga aacttgtctt ctctttcctt 5040
cacctcctct ctggcagctc tctgtactgg gatgctgctc tccaccaagc ccctcctcat 5100
atcatttacc tttgggagga gcttctccag cagctggaca acactgtgct gaaatcaacg 5160
tggagcaggg gaactatttc aaactcgtga ttgcaaagct tgcaatttct ttctattctc 5220
agttaaggac tgcgcaaaga caccttcagt aggaagcacg tggctcggag atctgccaaa 5280
agtacattcc tggccacaag gctctttcag gagtcaaaat attagctgcc cctccaaatt 5340
caggactcaa atccagccct ctggctgagt aaaagctgtg aagtagaact aacctgtact 5400
agcaggtgaa acattcattt ctaccaccac ttcaatcatt ccagcaagac agcaattatg 5460
gagacatctc ctgtcctcct tggtctggag gaggaggctt tgttgatatc cagaaaacct 5520
gaactgaata ccatttgaat ggagagaatt tcagaaggat tcacaaaaag gagaccaata 5580
ctgcttggat tctgccctgt aaatattatt tattttcctt ttgggaattc ttctatagaa 5640
<210> 2
<211> 339
<212> DNA
<213> Duck (DUCK)
<400> 2
atataaggta caccagtgtg gcaagctgtc tctcagattg catttgcttt cacggatcta 60
tttagtacta aaagaaaaag ggaaagggga agggaaaaaa aaaaagggaa aaaagctgca 120
ctgaatgtga gatcatgcaa aagctagcaa tctatgttta tatttacctg ttcatgctga 180
tttcagttga tccggtggct cttgatgacg gcagtcagcc cacagagaac gctgaaaaag 240
atggactgtg caatgcttgt acatggagac agaatacaaa atcttccaga atagaagcca 300
taaaaattca aatcctcagc aaactgcgcc tggaacaag 339
<210> 3
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 3
taaggtccgc cttgacctgt 20
<210> 4
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 4
cgggacattt tcatccagtg c 21
Claims (8)
1. A molecular marker for identifying duck slaughter traits based on a myostatin gene MSTN is characterized in that the molecular marker is T or C and is positioned at 458 th position of the myostatin gene MSTN.
2. The molecular marker for identifying the duck slaughter trait based on the myostatin gene MSTN according to claim 1, wherein the MSTN has a nucleotide sequence shown as SEQ ID No. 1.
3. Use of a molecular marker for identifying duck slaughter traits based on the myostatin gene MSTN as claimed in any of claims 1-2 for identifying meat duck slaughter traits.
4. An identification method for identifying a duck slaughter trait using a molecular marker according to any one of claims 1-2, comprising the steps of:
(1) extracting the total DNA of vein blood of the wings of the meat ducks;
(2) designing a specific amplification primer by taking the site where the molecular marker is located and a sequence consisting of upstream and downstream bases as a target sequence, and carrying out PCR amplification by using the specific amplification primer by taking the total DNA as a template to obtain an amplification product;
(3) carrying out genotyping detection and sequencing on the amplification product to obtain the molecular marker type of the meat duck to be detected;
(4) and judging the slaughtering traits of the meat ducks according to the type of the molecular marker.
5. The method for identifying the duck slaughter trait by using the molecular marker as claimed in claim 4, wherein the target sequence is shown as SEQ ID No. 2.
6. The method for identifying the slaughter duck traits by using the molecular marker as claimed in claim 5, wherein the specific amplification primer sequence is as follows:
SEQ ID NO.3：F1：TAAGGTCCGCCTTGACCTGT
SEQ ID NO.4：R1：CGGGACATTTTCATCCAGTGC。
7. the method for identifying the slaughter duck traits by using the molecular marker as claimed in claim 4, wherein the genotyping detection method comprises subjecting the PCR amplification product to non-denaturing polyacrylamide gel electrophoresis and silver staining to obtain an image, and performing genotyping according to the image:
(1) only contains 1 band, and is CC type;
(2) contains 2 thin strips, and is TT type;
(3) containing 1 coarse band and 1 fine band, is TC type.
8. The identification method for identifying the duck slaughter trait by using the molecular marker as claimed in claim 4, wherein the specific steps for judging the duck slaughter trait according to the type of the molecular marker in the step (4) are as follows:
(1) if the molecular marker type of the meat duck to be detected is TT type, the slaughtering character of the meat duck is the best;
(2) if the molecular marker type of the meat duck to be detected is CC type, the slaughtering character of the meat duck is the worst;
(3) if the molecular marker type of the meat duck to be detected is TC type, the slaughter traits of the meat duck are moderate.
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