CN112592852A - Culture medium and application thereof in culture of marine photosynthetic bacteria - Google Patents
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Abstract
The invention relates to the technical field of microorganisms, in particular to a culture medium and application thereof in culture of marine photosynthetic bacteria. The culture medium provided by the invention comprises water and: sodium acetate, yeast extract, peptone, sodium chloride, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ferric citrate, compound vitamins, compound trace elements and plant extract. The carbon source, the nitrogen source, the trace elements, the vitamins and the mixed bacteria inhibitor in the culture medium are reasonably selected, the nutrition is comprehensive, and the mixture ratio is proper. Can meet the requirement of continuous and rapid growth of photosynthetic bacteria in the photobioreactor, shortens the culture period and has the concentration far higher than that of the traditional culture mode. Moreover, the invention discovers that after the mango leaves and the honeysuckle extracts are added into the culture medium, the Chinese herbal medicines not only have the function of inhibiting the growth of mixed bacteria in the photosynthetic bacteria, but also can provide nutrients for the photosynthetic bacteria and effectively promote the rapid growth of the photosynthetic bacteria.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a culture medium and application thereof in culture of marine photosynthetic bacteria.
Background
Photosynthetic bacteria are a generic name for prokaryotic microorganisms that use light as an energy source and can perform photosynthesis under anaerobic illumination or aerobic dark conditions, and are distributed in soil, mire and marsh, seawater, fresh water, sludge, plant roots and the like. It plays an important role in water purification, water quality monitoring, feed additives, bacterial manure and other aspects.
Based on the application advantages of photosynthetic bacteria, the photosynthetic bacteria are required to be cultured on a large scale, the production cost can be saved by utilizing solar energy to carry out outdoor culture, but the outdoor environment is easy to pollute, the passage times are limited, and the photosynthetic bacteria are limited by natural weather, and the culture failure can be easily caused in a long-term cloudy day. The application of the photobioreactor can solve the problems, particularly the large-scale culture of the strains is suitable for being carried out in an indoor strict sterilization environment so as to reduce the pollution of mixed bacteria and increase the success rate of the expanded culture and the passage times of the strains; meanwhile, the photobioreactor can be continuously supplemented with light in cloudy days and at night, so that the culture speed and the final thallus concentration are greatly improved.
However, the research on the culture medium of photosynthetic bacteria is still insufficient, and the photosynthetic bacteria cannot be provided with sufficient nutrition. Therefore, further research on the culture medium is required to achieve rapid propagation of photosynthetic bacteria at high purity and high concentration.
Disclosure of Invention
In view of the above, the technical problem to be solved by the present invention is to provide a culture medium and its application in marine photosynthetic bacteria culture, in order to realize rapid propagation of photosynthetic bacteria with high purity and high concentration.
The culture medium provided by the invention comprises water and: 1.0-5.0 g/L of sodium acetate, 0.5-1.2 g/L of yeast extract, 0.1-0.5 g/L of peptone, 3.0-8.0 g/L of sodium chloride, 0.01-0.05 g/L of magnesium sulfate, 0.5-1.5 g/L of monopotassium phosphate, 0.1-0.5 g/L of dipotassium phosphate, 0.05-0.1 g/L of ferric citrate, 0.1-0.5 vol% of vitamin complex, 0.05-0.3 vol% of compound trace elements and 0.1-0.5 vol% of plant extract.
In the present invention, the multivitamins include water and vitamin B1Vitamin B12Biotin, nicotinic acid and folic acid. In some embodiments, the multivitamins include water and vitamin B1150mg/L, vitamin B120.5mg/L, biotin 2mg/L, nicotinic acid 0.1mg/L, folic acid 10 mg/L.
In the invention, the composite trace elements comprise water and Fe2+、Mn2+、Co2+、Cu2+、Zn2+And BO3 3-. In the examples of the present invention, the Fe2+From FeCl2Or FeCl2·4H2O; the Mn is2+From MnCl2Or MnCl2·4H2O, said Co2+From CoCl2Or CoCl2·6H2O, the Cu2+From CuSO4Or CuSO4·5H2O, said Zn2+From ZnSO4Or ZnSO4·7H2O, said BO3 3-From H3BO3. In some embodiments, the complex trace elements include water and FeCl2·4H2O 1500mg/L，MnCl2·4H2O 50mg/L，CoCl2·6H2O 200mg/L，CuSO4·5H2O 5mg/L，ZnSO4·7H2O 50mg/L，H3BO3 100mg/L。
In the invention, the plant extract is at least one selected from mango leaf extract and honeysuckle extract. In some embodiments, the plant extract is an ethanol extract of mango leaves and honeysuckle. In some embodiments, the plant extract is prepared by a method comprising: crushing mango leaves and honeysuckle, adding 10-20L of 75-80% ethanol solution per g of powder, centrifuging for 20-30 min at the rotating speed of 5000-8000 r/min, taking supernatant, and concentrating under reduced pressure until the volume is reduced by 70-80% to obtain the plant extract. The mass ratio of the mango leaves to the honeysuckle is 1: 1.
in some embodiments, the medium is composed of water and: 1.0g/L of sodium acetate, 3.0g/L of sodium chloride, 0.5g/L of yeast extract, 0.5g/L of peptone, 0.01g/L of magnesium sulfate, 0.5g/L of potassium dihydrogen phosphate, 0.1g/L of dipotassium hydrogen phosphate, 0.05g/L of ferric citrate, 5ml/L of compound vitamin, 3ml/L of compound trace element and 5ml/L of plant extract.
In some embodiments, the medium is composed of water and: 2.0g/L of sodium acetate, 4.0g/L of sodium chloride, 0.7g/L of yeast extract, 0.4g/L of peptone, 0.02g/L of magnesium sulfate, 1.0g/L of potassium dihydrogen phosphate, 0.3g/L of dipotassium hydrogen phosphate, 0.07g/L of ferric citrate, 4ml/L of compound vitamin, 2ml/L of compound trace element and 4ml/L of plant extract.
In some embodiments, the medium is composed of water and: 3.0g/L of sodium acetate, 5.0g/L of sodium chloride, 0.9g/L of yeast extract, 0.3g/L of peptone, 0.03g/L of magnesium sulfate, 1.2g/L of potassium dihydrogen phosphate, 0.4g/L of dipotassium hydrogen phosphate, 0.08g/L of ferric citrate, 3ml/L of compound vitamin, 1ml/L of compound trace element and 3ml/L of plant extract.
In some embodiments, the medium is composed of water and: 4.0g/L of sodium acetate, 7.0g/L of sodium chloride, 1.0g/L of yeast extract, 0.2g/L of peptone, 0.04g/L of magnesium sulfate, 1.4g/L of potassium dihydrogen phosphate, 0.4g/L of dipotassium hydrogen phosphate, 0.09g/L of ferric citrate, 2ml/L of compound vitamin, 1ml/L of compound trace element and 2ml/L of plant extract.
In some embodiments, the medium is composed of water and: 5.0g/L of sodium acetate, 8.0g/L of sodium chloride, 1.2g/L of yeast extract, 0.1g/L of peptone, 0.05g/L of magnesium sulfate, 1.5g/L of potassium dihydrogen phosphate, 0.5g/L of dipotassium hydrogen phosphate, 0.1g/L of ferric citrate, 1ml/L of compound vitamin, 0.5ml/L of compound trace element and 1ml/L of plant extract.
The preparation method of the culture medium comprises the following steps:
sterilizing water, and mixing with sodium acetate, yeast extract, peptone, sodium chloride, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ferric citrate, compound vitamins, compound trace elements and plant extract to obtain culture medium;
or mixing sodium acetate, yeast extract, peptone, sodium chloride, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ferric citrate, compound vitamins, compound microelements, plant extract and water, and sterilizing to obtain the culture medium.
The culture medium is applied to culturing photosynthetic bacteria.
The photosynthetic bacteria are selected from at least one of rhodopseudomonas palustris, rhodopseudomonas capsulata and rhodospirillum rubrum.
The invention also provides a culture method of photosynthetic bacteria, which is to inoculate strains in the culture medium for culture.
The culture method is suitable for culturing at least one photosynthetic bacterium selected from rhodopseudomonas palustris, rhodopseudomonas capsulata and rhodospirillum rubrum.
In the culture method, the inoculation amount of the inoculation is 25-40 vol%; in some embodiments, the inoculation amount for the inoculation is 35%. The inoculation refers to a step of adding the activated photosynthetic bacteria strain to a fresh medium. The culturing is carried out in a photobioreactor.
The culture medium provided by the invention comprises water and: sodium acetate, yeast extract, peptone, sodium chloride, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ferric citrate, compound vitamins, compound trace elements and plant extract. The carbon source, the nitrogen source, the trace elements, the vitamins and the mixed bacteria inhibitor in the culture medium are reasonably selected, the nutrition is comprehensive, and the mixture ratio is proper. Can meet the requirement of continuous and rapid growth of photosynthetic bacteria in the photobioreactor, shortens the culture period and has the concentration far higher than that of the traditional culture mode. Moreover, the invention discovers that after the mango leaves and the honeysuckle extracts are added into the culture medium, the Chinese herbal medicines not only have the function of inhibiting the growth of mixed bacteria in the photosynthetic bacteria, but also can provide nutrients for the photosynthetic bacteria and effectively promote the rapid growth of the photosynthetic bacteria.
Detailed Description
The invention provides a culture medium and application thereof in culture of marine photosynthetic bacteria, and a person skilled in the art can use the content for reference and appropriately improve process parameters to realize the culture. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
The test materials adopted by the invention are all common commercial products and can be purchased in the market.
The invention is further illustrated by the following examples:
example 1
1. Preparing a mixed bacteria inhibiting solution: mixing mango leaves and honeysuckle in a mass ratio of 1:1, crushing, sieving with a 800-100-mesh sieve, taking powder, adding 75-80% ethanol water solution according to a feed-liquid ratio (1: 10-20) g/L, centrifuging at a rotating speed of 5000-8000 r/min for 20-30 min, taking supernatant, and concentrating under reduced pressure until the volume is reduced by 70-80%, thereby obtaining a mixed extract of the mango leaves and the honeysuckle, which is used as a mixed bacteria inhibiting solution.
2. Water sterilization: boiling tap water, and cooling to below 35 deg.C;
or filtering tap water through two-stage micropores of 1 micron and 0.22 micron;
or preparing distilled water and ultrapure water.
3. Preparing a compound vitamin solution: vitamin B1150mg of vitamin B120.5mg, biotin 2mg, nicotinic acid 0.1mg, folic acid 10mg, constant volume 1L.
4. Preparing a composite trace element solution: FeCl2·4H2O 1500mg，MnCl2·4H2O 50mg，CoCl2·6H2O 200mg，CuSO4·5H2O 5mg，ZnSO4·7H2O 50mg，H3BO3100mg, constant volume 1L.
5. Preparing a culture medium: taking 1.0g of sodium acetate, 3.0g of sodium chloride, 0.5g of yeast extract, 0.5g of peptone, 0.01g of magnesium sulfate, 0.5g of monopotassium phosphate, 0.1g of dipotassium phosphate, 0.05g of ferric citrate, 5ml of compound vitamin, 3ml of compound trace element and 5ml of mixed bacteria inhibiting solution, and supplementing the water sterilized in the step 2 to 1L.
Example 2
1. Preparing mixed bacteria inhibiting solution, compound vitamin solution and compound trace element solution: the same as in example 1.
2. Preparing a culture medium: 2.0g of sodium acetate, 4.0g of sodium chloride, 0.7g of yeast extract, 0.4g of peptone, 0.02g of magnesium sulfate, 1.0g of monopotassium phosphate, 0.3g of dipotassium hydrogen phosphate, 0.07g of ferric citrate, 4ml of compound vitamin, 2ml of compound trace element and 4ml of mixed bacteria inhibiting solution, supplementing water (can be sterilized or tap water) to 1L and then carrying out autoclaving.
Example 3
1. Preparing mixed bacteria inhibiting solution, compound vitamin solution and compound trace element solution: the same as in example 1.
2. Water sterilization: the same as in example 1.
3. Preparing a culture medium: 3.0g of sodium acetate, 5.0g of sodium chloride, 0.9g of yeast extract, 0.3g of peptone, 0.03g of magnesium sulfate, 1.2g of monopotassium phosphate, 0.4g of dipotassium phosphate, 0.08g of ferric citrate, 3ml of compound vitamin, 1ml of compound trace element and 3ml of mixed bacteria inhibiting solution, and supplementing the water sterilized in the step 2 to 1L.
Example 4
1. Preparing mixed bacteria inhibiting solution, compound vitamin solution and compound trace element solution: the same as in example 1.
2. Preparing a culture medium: 4.0g of sodium acetate, 7.0g of sodium chloride, 1.0g of yeast extract, 0.2g of peptone, 0.04g of magnesium sulfate, 1.4g of monopotassium phosphate, 0.4g of dipotassium hydrogen phosphate, 0.09g of ferric citrate, 2ml of compound vitamin, 1ml of compound trace element and 2ml of mixed bacteria inhibiting solution, supplementing water (can be sterilized or tap water) to 1L and then carrying out autoclaving.
Example 5
1. Preparing mixed bacteria inhibiting solution, compound vitamin solution and compound trace element solution: the same as in example 1.
2. Water sterilization: the same as in example 1.
3. Preparing a culture medium: 5.0g of sodium acetate, 8.0g of sodium chloride, 1.2g of yeast extract, 0.1g of peptone, 0.05g of magnesium sulfate, 1.5g of monopotassium phosphate, 0.5g of dipotassium phosphate, 0.1g of ferric citrate, 1ml of compound vitamin, 0.5ml of compound trace element and 1ml of mixed bacteria inhibiting solution, and supplementing the water sterilized in the step 2 to 1L.
Comparative example 1
1. Preparing mixed bacteria inhibiting solution, compound vitamin solution and compound trace element solution: the same as in example 1.
2. Water sterilization: the same as in example 1.
3. Preparing a culture medium: 5.0g of sodium acetate, 8.0g of sodium chloride, 1.2g of yeast extract, 0.1g of peptone, 0.05g of magnesium sulfate, 1.5g of monopotassium phosphate, 0.5g of dipotassium phosphate, 0.1g of ferric citrate, 0.5ml of composite trace elements and 1ml of mixed bacteria inhibiting solution, and supplementing the water sterilized in the step 2 to 1L.
Comparative example 2
1. Preparing mixed bacteria inhibiting solution, compound vitamin solution and compound trace element solution:
2. water sterilization: the same as in example 1.
3. Preparing a culture medium: 5.0g of sodium acetate, 8.0g of sodium chloride, 1.2g of yeast extract, 0.1g of peptone, 0.05g of magnesium sulfate, 1.5g of monopotassium phosphate, 0.5g of dipotassium phosphate, 0.1g of ferric citrate, 1ml of compound vitamin and 0.5ml of compound trace element, and supplementing the water sterilized in the step 1 to 1L.
Comparative example 3
NH40.5g of Cl, 5.0g of NaCl, 1.0g of sodium acetate, 1.0g of yeast extract and CaCl2 0.2g、MgSO4·7H2O 0.2g、KH2PO4 0.2g、K2HPO40.2g, adding cold boiled water to 1L, and adjusting the pH to 7.4.
Comparative example 4
NH4Cl 0.5g, sodium acetate 1.0g, MgSO4·7H2O 0.2g、KH2PO4 0.3g、K2HPO40.3g, 0.5g of yeast extract, 0.5g of peptone, 1ml of composite vitamin stock solution, 20ml of trace element stock solution, adding cold boiled water to 1L, and adjusting the pH value to 7.4.
Compound vitamin stock solution: vitamin B10.1g, 0.1g nicotinic acid, 1.5mg biotin, and adding cold boiled water to 100 ml.
Microelement stock solution: 0.004g of copper nitrate heptahydrate, 0.024g of zinc sulfate heptahydrate, 0.075g of sodium molybdate dihydrate and 100ml of cold boiled water.
Test example 1:
the culture media of the above examples and comparative examples were compared for the effect of culturing photosynthetic bacteria.
A100L photobioreactor was used for the culture experiment. The culture media are prepared according to the proportion, the culture media are added into a photo-biological reaction device, 35% of activated photosynthetic bacteria strains are respectively inoculated, the culture volume is 100L, and each bacterial culture medium is tested for three times. The photosynthetic bacteria strain used in the test is Rhodopseudomonas palustris (Rhodopseudomonas palustris) which is obtained by screening from a Penaeus Hainanensis culture pond by the inventor.
Culturing in room with energy-saving lamp turned on and uninterrupted flow, sampling immediately after inoculation and the first, second, third and fourth days of culture, and measuring light absorption value (OD) at 660nm with spectrophotometer660) And is used for reflecting the biomass of the thallus. The results are shown in Table 1.
TABLE 1 Effect of different media on the growth rate of photosynthetic bacteria (Rhodopseudomonas palustris)
The results were similar after replacing Rhodopseudomonas palustris with Rhodopseudomonas capsulata (Rhodopseudomonas capsulata).
Rhodospirillum rubrum (Rhodospirillum rubrum) was selected for testing, and the test method was the same as above. The results are shown in Table 2.
TABLE 2 Effect of different media on the growth rate of photosynthetic bacteria (Rhodospirillum rubrum)
| Days of culture | Initial OD660 | Day one OD660 | The next day D660 | OD on day three660 | OD day four660 |
| Example 1 | 0.746±0.030 | 1.040±0.040ab | 1.358±0.042a | 1.607±0.075a | 1.801±0.059a |
| Example 2 | 0.742±0.019 | 1.056±0.018a | 1.377±0.014a | 1.624±0.022a | 1.823±0.016a |
| Example 3 | 0.749±0.047 | 1.053±0.048ab | 1.407±0.027a | 1.662±0.043a | 1.851±0.042a |
| Example 4 | 0.736±0.028 | 1.030±0.028ab | 1.388±0.017a | 1.670±0.017a | 1.874±0.018a |
| Example 5 | 0.755±0.037 | 1.061±0.039a | 1.398±0.023a | 1.679±0.025 | 1.878±0.031a |
| Comparative example 1 | 0.738±0.031 | 0.935±0.037c | 1.226±0.033b | 1.386±0.022b | 1.440±0.013b |
| Comparative example 2 | 0.757±0.018 | 0.963±0.020bc | 1.183±0.034b | 1.245±0.028c | 1.304±0.033c |
| Comparative example 3 | 0.740±0.028 | 0.899±0.021c | 1.089±0.036c | 1.152±0.043c | 1.246±0.032c |
| Comparative example 4 | 0.759±0.024 | 0.911±0.022c | 1.224±0.022b | 1.378±0.025b | 1.459±0.033b |
The results in tables 1 and 2 show that the growth rate of the photosynthetic bacteria of examples 1 to 5 is significantly higher than that of comparative examples 1 to 4, and the growth rate of the photosynthetic bacteria of comparative examples 1 to 4 is slow by the fourth day after the stationary phase is entered at the third day; the high growth continued from the days 1-5 to the fourth. The culture medium is designed according to the physiological characteristics and nutritional requirements of the photosynthetic bacteria under continuous artificial illumination culture, can meet the nutritional requirements of massive continuous proliferation of the photosynthetic bacteria, and enables the concentration of the bacterial liquid to reach a higher level after the stabilization period is prolonged.
The photosynthetic bacteria cultured in the culture media of examples 1-5 were tested for the rate of undesired bacteria, which were all less than 0.5%. As the mixed extract of honeysuckle and mango leaves is added into the culture medium of the embodiment 1-5, the growth speed of the photosynthetic bacteria is obviously better than that of the comparative example 2, and a large amount of photosynthetic bacteria can be cultured in a short period; the mixed extract of honeysuckle and mango leaves not only inhibits the negative influence brought by the growth of mixed bacteria, but also provides nutrient substances for the growth of photosynthetic bacteria.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that it is obvious to those skilled in the art that various modifications and improvements can be made without departing from the principle of the present invention, and these modifications and improvements should also be considered as the protection scope of the present invention.
Claims (10)
1. A culture medium comprising water and: 1.0-5.0 g/L of sodium acetate, 0.5-1.2 g/L of yeast extract, 0.1-0.5 g/L of peptone, 3.0-8.0 g/L of sodium chloride, 0.01-0.05 g/L of magnesium sulfate, 0.5-1.5 g/L of monopotassium phosphate, 0.1-0.5 g/L of dipotassium phosphate, 0.05-0.1 g/L of ferric citrate, 0.1-0.5 vol% of compound vitamin, 0.05-0.3 vol% of compound trace element and 0.1-0.5% of plant extract;
the vitamin complex comprises water and vitamin B1Vitamin B12Biotin, nicotinic acid and folic acid;
the compound trace elements comprise water and Fe2+、Mn2+、Co2+、Cu2+、Zn2+And BO3 3-；
The plant extract is at least one of mango leaf extract and honeysuckle extract.
2. The culture medium of claim 1, wherein the complex vitamins include water and vitamin B1150mg/L, vitamin B120.5mg/L, biotin 2mg/L, nicotinic acid 0.1mg/L, folic acid 10 mg/L.
3. The culture medium of claim 1, wherein the complex trace elements comprise water and FeCl2·4H2O 1500mg/L，MnCl2·4H2O 50mg/L，CoCl2·6H2O 200mg/L，CuSO4·5H2O 5mg/L，ZnSO4·7H2O 50mg/L，H3BO3 100mg/L。
4. The culture medium of claim 1, wherein the plant extract is an ethanol extract of mango leaves and honeysuckle flowers.
5. The culture medium of claim 4, wherein the plant extract is prepared by a method comprising: crushing mango leaves and honeysuckle, adding 10-20L of 75-80% ethanol solution per g of powder, centrifuging for 20-30 min at the rotating speed of 5000-8000 r/min, taking supernatant, and concentrating under reduced pressure until the volume is reduced by 70-80% to obtain a plant extract; the mass ratio of the mango leaves to the honeysuckle is 1: 1.
6. a method for preparing the medium according to any one of claims 1 to 5, comprising:
sterilizing water, and mixing with sodium acetate, yeast extract, peptone, sodium chloride, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ferric citrate, compound vitamins, compound trace elements and plant extract to obtain culture medium;
or mixing sodium acetate, yeast extract, peptone, sodium chloride, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ferric citrate, compound vitamins, compound microelements, plant extract and water, and sterilizing to obtain the culture medium.
7. Use of the culture medium according to any one of claims 1 to 5 for culturing photosynthetic bacteria.
8. Use according to claim 7, wherein the photosynthetic bacteria are selected from at least one of Rhodopseudomonas palustris, Rhodopseudomonas capsulata or Rhodospirillum rubrum.
9. A method for culturing photosynthetic bacteria, characterized in that a seed culture is inoculated into the culture medium according to any one of claims 1 to 5 and cultured.
10. The cultivation method according to claim 9, wherein the photosynthetic bacteria are selected from at least one of rhodopseudomonas palustris, rhodopseudomonas capsulata, or rhodospirillum rubrum; the inoculation amount of the inoculation is 25-40 vol%; the culturing is carried out in a photobioreactor.
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| CN113817646A (en) * | 2021-10-12 | 2021-12-21 | 中国水产科学研究院珠江水产研究所 | Simple photosynthetic bacteria culture medium and application thereof |
| CN114107126A (en) * | 2021-12-14 | 2022-03-01 | 上海荣吉明微生物工程有限公司 | Composite microbial inoculum for degrading pesticide residues and preparation method and application thereof |
| WO2022127229A1 (en) * | 2020-12-16 | 2022-06-23 | 中国热带农业科学院热带生物技术研究所 | Culture medium and applications thereof in culturing marine photosynthetic bacteria |
| CN115537357A (en) * | 2022-10-20 | 2022-12-30 | 刘正君 | Photosynthetic bacteria culture medium formula |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102326673A (en) * | 2011-06-18 | 2012-01-25 | 山西维民生科技有限公司 | Method for producing aerobic fermentation photosynthetic bacteria livestock culturing feed additive |
| CN103898022A (en) * | 2014-04-01 | 2014-07-02 | 湛江恒兴养殖技术服务有限公司 | Novel formula of photosynthetic bacteria culture medium and preparation method |
| CN110468087A (en) * | 2019-09-24 | 2019-11-19 | 中国热带农业科学院热带生物技术研究所 | A kind of photosynthetic bacteria culture medium and preparation method thereof simply easily made |
| CN111235071A (en) * | 2019-12-10 | 2020-06-05 | 黄河三角洲京博化工研究院有限公司 | Rhodopseudomonas palustris culture medium and application thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR0141660B1 (en) * | 1994-07-26 | 1998-06-15 | 한일성 | Method of producing pisciculture feed with photosythetic bacteria |
| CN1312273C (en) * | 2002-09-30 | 2007-04-25 | 中国科学院沈阳应用生态研究所 | Additive for photosyntheric bacterial culture medium |
| CN100575480C (en) * | 2007-03-21 | 2009-12-30 | 珠海市农业科学研究中心 | One plant height is imitated Rhodopseudomonas palustris and the application thereof that utilizes nitrite nitrogen |
| CN112592852A (en) * | 2020-12-16 | 2021-04-02 | 中国热带农业科学院热带生物技术研究所 | Culture medium and application thereof in culture of marine photosynthetic bacteria |
-
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102326673A (en) * | 2011-06-18 | 2012-01-25 | 山西维民生科技有限公司 | Method for producing aerobic fermentation photosynthetic bacteria livestock culturing feed additive |
| CN103898022A (en) * | 2014-04-01 | 2014-07-02 | 湛江恒兴养殖技术服务有限公司 | Novel formula of photosynthetic bacteria culture medium and preparation method |
| CN110468087A (en) * | 2019-09-24 | 2019-11-19 | 中国热带农业科学院热带生物技术研究所 | A kind of photosynthetic bacteria culture medium and preparation method thereof simply easily made |
| CN111235071A (en) * | 2019-12-10 | 2020-06-05 | 黄河三角洲京博化工研究院有限公司 | Rhodopseudomonas palustris culture medium and application thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022127229A1 (en) * | 2020-12-16 | 2022-06-23 | 中国热带农业科学院热带生物技术研究所 | Culture medium and applications thereof in culturing marine photosynthetic bacteria |
| CN113817646A (en) * | 2021-10-12 | 2021-12-21 | 中国水产科学研究院珠江水产研究所 | Simple photosynthetic bacteria culture medium and application thereof |
| CN114107126A (en) * | 2021-12-14 | 2022-03-01 | 上海荣吉明微生物工程有限公司 | Composite microbial inoculum for degrading pesticide residues and preparation method and application thereof |
| CN115537357A (en) * | 2022-10-20 | 2022-12-30 | 刘正君 | Photosynthetic bacteria culture medium formula |
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