CN109350576B

CN109350576B - Composition with moisturizing effect and application thereof, toning lotion and preparation method thereof

Info

Publication number: CN109350576B
Application number: CN201811524348.5A
Authority: CN
Inventors: 凌远强
Original assignee: Guangzhou Uzise Biotechnology Co ltd
Current assignee: Guangzhou Uzise Biotechnology Co ltd
Priority date: 2018-12-13
Filing date: 2018-12-13
Publication date: 2021-07-30
Anticipated expiration: 2038-12-13
Also published as: CN109350576A

Abstract

The invention relates to a composition with moisturizing effects, application of the composition, toning lotion and a preparation method of the toning lotion. The composition comprises: 40-60 parts of yeast extract; 30-50 parts of water; 1-5 parts of butanediol; 1-5 parts of propylene glycol; 0.01-0.1 part of citric acid; 0.01-0.1 part of lactic acid; 0.1-1.1 parts of glycerol; 0.01-0.1 part of sodium citrate; 0.1-1 part of panthenol; 0.1-0.8 part of sodium hyaluronate; 0.01-0.05 part of glycerol phosphoinositide choline salt; 1-5 parts of a filtrate of a fermentation product of the leuconostoc/radish root; 0.5-2.5 parts of purslane extract; 0.5-2.5 parts of twisted cactus stem extract; 0.1-0.4 parts of pomelo fruit extract; 0.2-2.2 parts of lotus extract; 0.1-1 part of plum fruit extract. The composition is safe and non-irritant, and has strong effects of moisturizing.

Description

Composition with moisturizing effect and application thereof, toning lotion and preparation method thereof

Technical Field

The invention relates to the technical field of cosmetics, in particular to a composition with moisturizing effects and an application thereof, a toning lotion and a preparation method thereof.

Background

With the continuous improvement of living standard of people, the market demand of beauty cosmetics continuously rises, and especially women pay high attention to the beauty cosmetics, which lays a solid foundation for the rapid development of beauty cosmetics industry.

The stratum corneum, which is the top layer of human skin, has water-absorbing and barrier functions, and amino acids, lactate, saccharides, and the like contained in the stratum corneum maintain a certain water content in the stratum corneum to maintain skin moisturization. Furthermore, the appearance of the skin is related to the moisture content of the stratum corneum, which normally has 10% to 30% moisture to maintain the softness and elasticity of the skin. Therefore, cosmetics with moisturizing effects are increasingly regarded by people. For a long time, the cosmetic water is a kind of cosmetic which is highly popular among consumers, can sufficiently replenish the moisture in the horny layer of the skin, and can be used for cleaning the skin and keeping the skin healthy. Meanwhile, the toning lotion also has the effect of adjusting the pH value and can adjust the pH value of the skin. However, the existing commercially available lotion has a stimulating effect on the skin and has no obvious moisturizing effect.

Disclosure of Invention

Based on the above, it is necessary to provide a safe and non-irritant composition with moisturizing effect and application thereof, a lotion and a preparation method thereof aiming at the problems that the lotion can stimulate the skin and the moisturizing effect is not obvious

The composition with the effects of moisturizing comprises the following components in parts by weight:

the composition with the effects of moisturizing is not added with irritant components, such as: alcohol, salicylic acid and other raw materials, and has the advantages of safety and no irritation. In addition, the composition contains yeast extract components in a reasonable proportion, and the yeast extract and other components are cooperated to endow the composition with strong water replenishing and moisturizing effects, so that the defects of dull and rough complexion and lusterless skin can be effectively improved, the skin becomes white, red and elastic, the skin aging is slowed down, the skin wrinkles and color spots are reduced, and the effects of whitening, brightening the skin, removing the wrinkles and resisting aging are achieved.

In one embodiment, the composition with the moisturizing effect comprises the following components in parts by mass:

an application of the composition with the effects of moisturizing and nourishing water in preparation of cosmetics.

A toning lotion with moisturizing effect is prepared from the composition with moisturizing effect.

The toning lotion with the moisturizing effect is not added with irritant components, such as: alcohol, salicylic acid and other raw materials, and has the advantages of safety and no irritation. In addition, the toning lotion contains yeast extract components with reasonable proportion, and the yeast extract and other components are cooperated to endow the toning lotion with strong water replenishing and moisture keeping effects, so that the defects of dull, rough and lusterless complexion can be effectively improved, the skin becomes white, ruddy and elastic, the skin aging is slowed down, the skin wrinkles and color spots are reduced, and the toning lotion has the effects of whitening, brightening, removing wrinkles and resisting aging.

The preparation method of the toning lotion with the effects of moisturizing comprises the following steps:

adding water into a container, stirring and sequentially adding butanediol, propylene glycol, glycerol and sodium hyaluronate, heating to 80-85 ℃, and completely dissolving to obtain A;

cooling the A to 45-50 ℃, then sequentially adding yeast extract, the filtrate of the fermentation product of the leuconostoc/radish root, purslane extract, the stem extract of the twisted prickly pear, pomelo fruit extract, lotus flower extract, plum fruit extract and panthenol, and uniformly mixing to obtain B; and

and cooling the B to 35-40 ℃, adding citric acid, lactic acid, sodium citrate and glycerophosphoinositide choline salt, uniformly mixing, filtering and retaining the filtrate to obtain the toning lotion with the effects of moisturizing.

A cosmetic is prepared from the composition with the effects of moisturizing.

The cosmetic has strong effects of moisturizing, effectively improving the defects of dull, rough and lusterless complexion, whitening and moistening skin, having elasticity, slowing down skin aging, reducing skin wrinkles and color spots, whitening and brightening skin, removing wrinkles and resisting aging.

Drawings

Fig. 1 is a flowchart illustrating a method for preparing a lotion having moisturizing effects according to an embodiment of the present invention.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

wherein yeast extract, purslane extract, prickly pear stem extract, pomelo fruit extract, lotus flower extract, plum fruit extract, citric acid, lactic acid, glycerin and glycerophosphoinositide choline salt are used as skin conditioners. Butanediol, propylene glycol, panthenol and sodium hyaluronate have moisturizing effect. Water is used as the solvent. The filtrate of the fermentation product of the leuconostoc/radish root is used as a natural preservative. Sodium citrate is used as pH regulator.

Wherein, the yeast extract has natural, safe and powerful moisturizing function, whitening function and anti-aging effect.

Firstly, yeast cells are rich in Natural Moisturizing Factors (NMF), such as amino acids, peptides, sodium, potassium, magnesium and other mineral elements. Wherein, the amino acid accounts for 40% of the Natural Moisturizing Factor (NMF), and the mineral elements such as sodium, potassium, magnesium and the like account for 12% of the Natural Moisturizing Factor (NMF). The Natural Moisturizing Factor (NMF) has the advantages of good skin affinity, high safety and the like, can rapidly penetrate through the skin to provide nutrients required by the skin, and deeply nourishes skin cells. And the major components of yeast cell wall include yeast beta- (1,3)/(1,6) -glucan and mannan M60. The yeast beta- (1,3)/(1,6) -glucan contains a large number of hydrophilic groups and can absorb water or lock water in the skin stratum corneum. The specific molecular structure of the yeast mannose M60 can lock water molecules to achieve long-acting moisturizing effect, and in addition, the mannan protein can provide moisture required by skin. Therefore, the yeast extract has natural, safe and strong moisturizing function.

Secondly, the color of the skin is mainly determined by the amount of melanin content in the epidermis. Melanin is formed in melanocytes at the basal part of the epidermis, then transferred to basal cells, carried to the epidermal layer as epidermal cells migrate, and finally lost as keratinized cells are exfoliated. The mechanism of melanin formation is that tyrosine in melanocytes is oxidized into dopa, dopaquinone, dopachrome, 5, 6-dihydroxyindole, indole-5, 6-quinone and the like under the influence of various factors in the environment under the action of tyrosinase, and finally converted into pigment particles. In addition to tyrosinase, 3 enzymes play a non-negligible role in melanin synthesis, namely dopachrome tautomerase, 5, 6-dihydroxyindole-2-carboxylate oxidase and peroxidase. The whitening agent can reduce the generation of melanin by inhibiting the activity of tyrosinase, another 3 enzymes or melanin oxidation pathway. Yeast extract is an active substance extracted from natural yeast, and is mainly obtained by degrading proteins in yeast cells into amino acids and peptides, degrading nucleic acids into nucleotides, and extracting with other active substances in yeast cells. At present, the whitening effect of the pure natural yeast extract is verified through in vitro experiments and human body experiments respectively. The results of the ex vivo tissue section experiments showed that the melanin inhibition rates of the yeast extract aqueous solutions with a mass fraction of 0.5% and 1.0% were 55.8% (p < 0.05) and 81.2% (p < 0.01), respectively, and the melanin inhibition rate of the kojic acid aqueous solution with a mass fraction of 1.0% (positive control) was 25.9% (p < 0.05), as compared with the placebo. In a double-blind human experiment, the skin brightness of the cream whitener using a yeast extract with a mass fraction of 3% showed a trend of increase over the whole test period, compared to the skin on the side using placebo, and still maintained an increase of more than 30% (p < 0.05) by test 42 d; the Melanin Index (MI) value of the skin tended to decrease throughout the test period, with a 38% decrease remaining by 42d (p < 0.05). The whitening effect of the yeast extract is obviously higher than that of a control group (p is less than 0.05).

Thirdly, among yeast extracts, the most prominent anti-aging effect is β - (1,3) -glucan produced by yeast. This is a very potent stimulator of the immune system, as it has the effect of activating macrophages, neutrophils and the unique immune cells in the skin, langerhans cells, and inducing local or systemic immune and repair functions. The mechanism is due to the presence of receptors on the surface of langerhans cells that specifically respond to beta-glucans, thereby stimulating langerhans cells to activate an immune response. Other glucans, such as mannans, galactans and alpha- (1,4) or beta- (1,4) linked glucans do not have this activity. Among them, the decrease of Langerhans cells is closely related to skin aging, and thus it is known that yeast β - (1,3) -glucan has anti-aging effects in addition to immune and repair. However, glucan isolated from the cell wall of baker's yeast is a water-insoluble particulate polymer and is not suitable for use in cosmetics. Thus, techniques have been developed to modify pure β - (1,3) -glucan to carboxymethylated glucan (CM-glucan). Carboxymethylation is carried out under specific conditions, and the degree of substitution can reach 0.75. It is this configuration which is sufficient to render the substance water soluble without destroying its original helical structure, so that it has a particular activity. CM-dextran is soluble in the concentration range of 4% and meets cosmetic requirements. It can be compatible with most of cosmetic components, and most of production process conditions are not affected.

Preferably, the composition with the moisturizing effect comprises the following components in parts by mass:

The composition with the moisturizing effect is applied to the preparation of cosmetics.

The toning lotion with the moisturizing effect is prepared from the composition with the moisturizing effect.

Referring to fig. 1, a preparation method of the lotion with moisturizing effect according to an embodiment of the present invention includes the following steps:

s10, adding water into a container, stirring and sequentially adding butanediol, propylene glycol, glycerol and sodium hyaluronate, heating to 80-85 ℃, and completely dissolving to obtain A.

S20, cooling the A to 45-50 ℃, sequentially adding yeast extract, the filtrate of the enterococcus casseliflavus/radish root fermentation product, purslane extract, the stem extract of the prickly pear, pomelo fruit extract, lotus flower extract, plum fruit extract and panthenol, and uniformly mixing to obtain the B.

S30, cooling the B to 35-40 ℃, adding citric acid, lactic acid, sodium citrate and glycerol phosphoinositide choline salt, uniformly mixing, filtering and retaining the filtrate to obtain the toning lotion with the effects of moisturizing.

Wherein the filtering operation is as follows: filtering with 400 mesh filter screen, and retaining the filtrate.

And after the steps are finished, storing the prepared toning lotion, then reporting to inspect, and after the toning lotion is inspected to be qualified, filling, packaging and warehousing.

The preparation method of the toning lotion with the moisturizing effect is simple in preparation process, and the prepared toning lotion has uniformly dispersed components and stable properties.

The cosmetic provided by the embodiment of the invention is prepared from the composition with the moisturizing effect.

The following are specific embodiments:

the starting materials used in the following examples are all commercially available.

The names of the ingredients used in the following examples are those specified by the international nomenclature for cosmetic materials (INCI): s. Minuisorba/radish root fermentation product filtrate.

Examples 1 to 3

The example 3 cosmetic lotion with moisturizing effect is prepared from the components shown in table 1:

TABLE 1 Components of lotions of examples 1 to 3

Components	Example 1	Example 2	Example 3
				Yeast extract	50g	40g	60g
Water (W)	36g	30g	42g
				Butanediol	3g	2g	4g
Propylene glycol	2g	3g	2g
				Citric acid	0.06g	0.03g	0.07g
Lactic acid	0.06g	0.05g	0.03g
				Glycerol	0.6g	0.8g	0.6g
Citric acid sodium salt	0.05g	0.03g	0.04g
				Panthenol	0.5g	0.6g	0.6g
Hyaluronic acid sodium salt	0.3g	0.5g	0.3g
				Glycerol phosphoinositide choline salt	0.03g	0.01g	0.02g
S. Ming-Shaw/radish root fermentation product filtrate	2.5g	2g	4g
				Purslane extract	1.5g	2g	1.6g
Opuntia Dillenii stem extract	1.5g	1.8g	1.3g
				Pomelo fruit extract	0.2g	0.2g	0.3g
Lotus flower extract	1.2g	1.4g	2.1g
				Plum extract	0.5g	0.8g	0.3g

The preparation method of the toning lotion of the above examples 1 to 3 is as follows:

adding water into a container, stirring and sequentially adding butanediol, propylene glycol, glycerol and sodium hyaluronate, heating to 85 ℃, and completely dissolving to obtain A.

Cooling A to 45 deg.C, sequentially adding yeast extract, Pleurotus Citrinopileatus Sing/radix Raphani fermentation product filtrate, herba Portulacae extract, caulis Opuntiae Dillenii stem extract, fructus Citri Grandis extract, flos Nelumbinis extract, mume fructus extract and panthenol, and mixing to obtain B.

Cooling B to 40 deg.C, adding citric acid, lactic acid, sodium citrate and glyceryl phosphoinositide choline salt, mixing, and filtering with 400 mesh filter screen.

Comparative example 1

A cosmetic lotion of comparative example 1, whose composition and preparation method are the same as those of example 1, is different in that: the yeast extract content is 10 g.

Comparative example 2

A cosmetic lotion of comparative example 2, whose composition and preparation method are the same as those of example 1, is different in that: the yeast extract content is 20 g.

Comparative example 3

A cosmetic lotion of comparative example 3, whose composition and preparation method are the same as those of example 1, is different in that: the yeast extract content is 70 g.

Comparative example 4

A cosmetic lotion of comparative example 4, whose composition and preparation method are the same as those of example 1, is different in that: sodium hyaluronate was not added.

Comparative example 5

A cosmetic lotion of comparative example 5, whose composition and preparation method are the same as those of example 1, is different in that: butanediol was not added.

And (3) performance testing:

1. skin seal patch test

The test substance was the lotions of examples 1 to 3 of the present invention.

The subjects were 30 volunteers with healthy skin, divided into 3 groups of 10.

The test method comprises the following steps: putting 0.1g-0.3g of the test object into a spot tester, then applying the spot tester with the test object on the back or the curved side of the forearm of the subject by using a non-irritating adhesive tape, and lightly pressing with the palm to uniformly apply the spot tester on the skin for 24 hours. The test substance is removed after 24h, the skin reaction is observed at 0.5h, 12h, 24h and 48h after the patch is removed, the results are recorded according to the skin reaction grading standard in the cosmetic hygiene standard (2007 edition), the identification standard is shown in table 2, and the test result is shown in table 3.

TABLE 2 identification standards

Grade 0	Has no irritation and erythema
		Grade 1	Weak erythema, dry skin, wrinkling
Grade 2	Erythema, edema, pimple, wheal, desquamation, fissure
		Grade 3	Erythema and blister
Grade 4	Severe erythema, edema, bulla, erosion, pigmentation or hypopigmentation

TABLE 3 Patch test results

As can be seen from Table 3, the lotions of examples 1 to 3 of the present invention did not cause any allergy or discomfort to the human body. Thus, the toning lotion is safe and non-irritant to skin.

2. Moisture retention efficacy test

Experimental samples: the lotions of examples 1 to 3 of the present invention and those of comparative examples 1 to 5.

The test method comprises the following steps: 80 subjects aged 20 to 40 years were selected and randomly divided into 8 groups of 10, each group having no significant difference in age and skin properties and the like and being comparable, and 8 groups of subjects used the lotions of examples 1 to 3 of the present invention and the lotions of comparative examples 1 to 5, respectively.

The specific method comprises the following steps: after cleaning the face, the subject was allowed to sit still in a constant environment for 30 minutes, and the measurement of the blank value of the test site was performed using a skin moisture content tester, and 5 points were fixedly measured for each area in a certain order to obtain an average value. The test specimens were then evenly smeared with latex gloves and the timer was started. The change in MMV values was measured at 0.5h, 1h, 3h and 6h, respectively. Subtracting the blank value from the average value of each detection to obtain the change of the MMV value in the time period, removing the blank value to obtain the increase rate of the MMV value, and averaging the calculation results of 10 subjects in each group. The moisture growth rate results are shown in Table 4.

The skin moisture content increase rate calculation formula is as follows:

skin moisture content increase rate = (MMVt-MMV0)/MMV0 × 100%;

MMV 0: MMV on the skin before smearing; MMVt: MMV of the skin t time after application.

TABLE 4 moisture growth rate

Item	0h	0.5h	1h	3h	6h
						Example 1	0	74.06	61.88	36.45	27.86
Example 2	0	70.25	60.51	34.15	23.72
						Example 3	0	71.12	62.09	35.24	26.37
Comparative example 1	0	53.42	38.26	19.78	8.53
						Comparative example 2	0	63.26	39.31	20.51	9.67
Comparative example 3	0	70.32	54.29	25.26	11.42
						Comparative example 4	0	67.29	58.79	32.14	20.49
Comparative example 5	0	68.09	57.68	31.89	20.51

As can be seen from the experimental results of Table 4, the moisturizing effects of the lotions of examples 1 to 3 of the present invention are much better than those of the lotions of comparative examples 1 to 5. The moisture growth rate can be kept high within 1h, and when the moisture growth rate lasts for 6h, the moisture growth rate is relatively high, and the moisture can be kept continuously. In particular, the cosmetic water of example 1 was most effective in replenishing water and moisturizing.

As is clear from comparison of example 1 with comparative examples 1 to 2, when the yeast extract was added to the cosmetic water in an amount of 10g and 20g, the water content increase rate decreased rapidly for 1 hour, and decreased even for 6 hours, and the moisturizing effect was poor.

As is clear from comparison between example 1 and comparative example 3, when 70g of yeast extract was added to the makeup water, the moisture growth rate was high when the duration was 0.5h, but the moisture growth rate was low when the duration was 3h, and the moisture growth rate was low when the duration was 6h, and the moisturizing effect was poor.

As can be seen from comparison of example 1 with comparative examples 4 to 5, when sodium hyaluronate or butanediol was not added to the lotion, the rate of water increase was relatively low when the lotion was maintained for 1 hour, and the effect of moisturizing was not as good as that of example 1 when the lotion was maintained for 6 hours.

Therefore, the toning lotion provided by the invention adopts the yeast extract with a reasonable proportion and the synergistic effect of the yeast extract and each component, so that the moisturizing effect is good.

3. Test of use effect

The experimental method comprises the following steps: 160 subjects aged 25-40 were selected, randomized into 8 groups of 20 subjects each, and subjects were not aware of the composition of the product, excluding the influence of psychological factors on the overall evaluation of the product.

The results of the use of the products were evaluated after 8 weeks of continuous use in the morning and evening with each of the lotions of examples 1 to 3 and comparative examples 1 to 5 for 8 subjects, and the results of the use evaluation were averaged for 20 subjects per group. The evaluation indexes of the using effect are moisture retention, absorption and using skin feel, and the evaluation of the using effect is three grades which are respectively as follows: the effect is not obvious (0-30 min), the effect is general (30-60 min), the effect is obvious (60-100 min), and the evaluation result of the use effect is shown in table 5.

Table 5 evaluation results of usage effects

Item	Degree of absorption	Moisture retention	Feeling of use
				Example 1	90.3	91.1	88.5
Example 2	85.6	83.7	87.3
				Example 3	80.4	89.1	85.4
Comparative example 1	88.2	29.1	54.1
				Comparative example 2	85.3	30.0	55.2
Comparative example 3	26.8	55.2	55.8
				Comparative example 4	80.2	78.5	85.2
Comparative example 5	78.6	76.5	81.6

As can be seen from the experimental results of Table 5, the lotions of examples 1 to 3 of the present invention had good moisturizing effect, were easy to absorb, and had good skin feel, and particularly the lotion of example 1 had the most significant effect in use.

As can be seen from comparative examples 1-2, when the yeast extract was added to the lotions in an amount of 10g to 20g, the resulting lotions were easily absorbed, but the skin feel was general and the moisture retention was poor.

As can be seen from comparative example 3, when the yeast extract was added to the cosmetic water in an amount of 70g, the resulting cosmetic water was not easily absorbed, and the moisturizing effect and the feeling of the skin upon application were general.

As can be seen from comparison of example 1 with comparative examples 4 to 5, when sodium hyaluronate or butanediol was not added to the lotion, the effect of the lotion of example 1 was not as good as the evaluation result, although the moisturizing property, the absorbency, and the feeling of the skin in use were good.

Therefore, the toning lotion disclosed by the invention adopts the yeast extract with a reasonable ratio and the synergistic effect of the yeast extract and each component, so that the toning lotion is easy to absorb by the skin, good in moisturizing effect and good in skin feel.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. The composition with the effects of moisturizing is characterized by comprising the following components in parts by weight:

2. the composition with the effects of moisturizing and preserving moisture as claimed in claim 1, is characterized by comprising the following components in parts by mass:

3. the composition with the effects of moisturizing and preserving moisture as claimed in claim 1, is characterized by comprising the following components in parts by mass:

4. use of the composition having moisturizing effect according to any one of claims 1 to 3 for preparing cosmetics.

5. A toning lotion with moisturizing effect, which is characterized by being prepared from the composition with moisturizing effect of any one of claims 1 to 3.

6. A preparation method of the toning lotion with the effects of moisturizing and preserving moisture as claimed in claim 5, which is characterized by comprising the following steps:

7. A cosmetic characterized by being prepared from the composition having moisturizing effect according to any one of claims 1 to 3.