CN108345914B - Sequencing consumable tracking method and sequencing system - Google Patents

Sequencing consumable tracking method and sequencing system Download PDF

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Publication number
CN108345914B
CN108345914B CN201710050224.7A CN201710050224A CN108345914B CN 108345914 B CN108345914 B CN 108345914B CN 201710050224 A CN201710050224 A CN 201710050224A CN 108345914 B CN108345914 B CN 108345914B
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consumable
value
information
standard
item
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CN108345914A (en
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徐剑峰
孙瑞涛
周志良
姜泽飞
颜钦
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Genemind Biosciences Co Ltd
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Genemind Biosciences Co Ltd
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Priority to CN201710050224.7A priority Critical patent/CN108345914B/en
Priority to PCT/CN2018/077386 priority patent/WO2018133884A1/en
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    • GPHYSICS
    • G06COMPUTING; CALCULATING OR COUNTING
    • G06KGRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
    • G06K17/00Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations
    • G06K17/0022Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations arrangements or provisions for transferring data to distant stations, e.g. from a sensing device
    • G06K17/0029Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations arrangements or provisions for transferring data to distant stations, e.g. from a sensing device the arrangement being specially adapted for wireless interrogation of grouped or bundled articles tagged with wireless record carriers
    • GPHYSICS
    • G06COMPUTING; CALCULATING OR COUNTING
    • G06KGRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
    • G06K17/00Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • Computer Networks & Wireless Communication (AREA)
  • General Engineering & Computer Science (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a tracking method and a sequencing system of sequencing consumables, wherein the tracking method comprises the following steps: before the consumable is placed on a sequencing system and sequencing is carried out, reading an information item and a value of the information item of the consumable by using an information reading and writing device to obtain an information tag of the consumable, wherein the information item comprises at least one of a type, a detection item and an identification position, the value of the type comprises a reaction device and a first reagent, the value of the detection item comprises prenatal detection and tumor screening, and the value of the identification position comprises used and unused; comparing the information tag of the consumable with the standard information tag of the consumable to obtain a comparison result; judging whether the consumables are normal or not according to the comparison result: when the information tag of the consumable is consistent with the standard information tag of the consumable, judging that the consumable is normal and prompting that the consumable is normal by using a prompting device; when the information label of consumptive material is inconsistent with the standard information label of consumptive material, judge that the consumptive material is unusual and utilize suggestion device suggestion consumptive material unusual. The tracking method can improve the accuracy of sequencing.

Description

Sequencing consumable tracking method and sequencing system
Technical Field
The invention relates to the technical field of gene sequencing, in particular to a tracking method and a sequencing system of sequencing consumables.
Background
In the related art, the materials used in the sequencing system include disposable consumables, such as reagent tubes containing reagents satisfying a specific data volume requirement/sequencing volume for a test item, and thus errors in consumable material classification negligence, errors in human memory resolution, or errors in placement operations are likely to occur during the use process, which may result in sequencing failure, relatively large amounts of invalid or low-quality sequencing data, data not available for subsequent analysis, and the like.
Disclosure of Invention
The embodiment of the invention aims to solve at least one technical problem in the prior art. For this reason, the embodiments of the present invention need to provide a method for tracking a sequencing consumable and a sequencing system.
The embodiment of the invention provides a tracking method of sequencing consumables, wherein the consumables are placed on a sequencing system during sequencing, the sequencing system comprises an information reading and writing device and a prompting device, and the tracking method comprises the following steps:
before the consumable is placed on the sequencing system and sequencing is carried out, reading an information item of the consumable and a value of the information item by using the information reading and writing device to obtain an information tag of the consumable, wherein the information tag of the consumable comprises the information item of the consumable and the value of the information item, the information item comprises at least one of a category, a detection item and an identification bit, the value of the category comprises a reaction device and/or a first reagent matched with the sequencing system, the value of the detection item comprises at least one of a disease genetic detection item, and the value of the identification bit comprises used and unused;
comparing the information tag of the consumable with a standard information tag of the consumable to obtain a comparison result, wherein the standard information tag of the consumable comprises an information item of the consumable and a standard value of the information item, and the information item in the standard information tag of the consumable covers the information item in the information tag of the consumable;
according to the comparison result, judging whether the consumable is normal:
when the information label of the consumable is consistent with the standard information label of the consumable, judging that the consumable is normal and prompting that the consumable is normal by using the prompting device; when the values of all corresponding information items in the information tag of the consumable part and the standard information tag of the consumable part are completely the same, the information tag of the consumable part and the standard information tag of the consumable part are regarded as consistent;
when the information tag of the consumable is inconsistent with the standard information tag of the consumable, judging that the consumable is abnormal and prompting the abnormality of the consumable by using the prompting device; and when the value of any corresponding information item in the information tag of the consumable part and the value of any corresponding information item in the standard information tag of the consumable part are different, the information tag of the consumable part and the standard information tag of the consumable part are regarded as inconsistent.
According to the tracking method of the sequencing consumables, the information reading and writing device is used for reading the information tags of the consumables, and the prompting device is used for prompting the state of the consumables, so that the sequencing consumables are clear and traceable, sequencing result errors caused by artificial distinguishing or loading errors are avoided, the requirement for attributing the sequencing consumables in different types and/or different states of the same type is lowered, and the sequencing consumables can be simply and effectively realized.
The sequencing system comprises a control device, an information reading and writing device and a prompting device, wherein the control device is connected with the information reading and writing device and the prompting device, before the consumable is placed on the sequencing system and sequencing is performed, the control device is used for reading an information item and a value of the information item of the consumable by using the information reading and writing device to obtain an information tag of the consumable, the information tag of the consumable comprises the information item and the value of the information item of the consumable, the information item comprises at least one of a type, a detection item and an identification bit, the value of the type comprises a reaction device and/or a first reagent matched with the sequencing system, and the value of the detection item comprises at least one of a disease genetic detection item, the value of the identification bit comprises used and unused;
the control device is used for comparing the information tag of the consumable with the standard information tag of the consumable to obtain a comparison result, the standard information tag of the consumable comprises the information item of the consumable and the standard value of the information item, and the information item in the standard information tag of the consumable covers the information item in the information tag of the consumable;
according to the comparison result, judging whether the consumable is normal:
when the information label of the consumable is consistent with the standard information label of the consumable, judging that the consumable is normal and prompting that the consumable is normal by using the prompting device; when the values of all corresponding information items in the information tag of the consumable part and the standard information tag of the consumable part are completely the same, the information tag of the consumable part and the standard information tag of the consumable part are regarded as consistent;
when the information tag of the consumable is inconsistent with the standard information tag of the consumable, judging that the consumable is abnormal and prompting the abnormality of the consumable by using the prompting device; and when the value of any corresponding information item in the information tag of the consumable part and the value of any corresponding information item in the standard information tag of the consumable part are different, the information tag of the consumable part and the standard information tag of the consumable part are regarded as inconsistent.
Among the above-mentioned sequencing consumptive material, utilize information read write device to read the information label of consumptive material to utilize suggestion device suggestion consumptive material's state, make the sequencing consumptive material clear and traceable, avoided the sequencing result mistake that leads to for distinguishing or loading the mistake artificially, reduced the requirement of putting in order to the sequencing consumptive material of different types and/or the different states of same type, be favorable to simply realizing the sequencing effectively.
The embodiment of the invention provides a control device for tracking sequencing consumables on a sequencing system, wherein the sequencing system comprises an information reading and writing device and a prompting device, and the control device comprises:
a storage unit for storing data, the data comprising a computer executable program;
a processor configured to execute the computer-executable program, wherein executing the computer-executable program comprises performing the method of any of the above embodiments.
A computer-readable storage medium of an embodiment of the present invention stores a program for execution by a computer, and executing the program includes performing the method of any of the above embodiments. The computer-readable storage medium may include: read-only memory, random access memory, magnetic or optical disk, and the like.
Additional aspects and advantages of embodiments of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of embodiments of the invention.
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The above and/or additional aspects and advantages of embodiments of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a schematic flow diagram of a method for tracking sequencing consumables according to an embodiment of the present invention;
FIG. 2 is a block diagram of a sequencing system according to an embodiment of the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "first", "second" and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, features defined as "first", "second", may explicitly or implicitly include one or more of the described features. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, "connected" is to be understood in a broad sense, e.g., fixedly, detachably or integrally connected; may be mechanically connected, may be electrically connected or may be in communication with each other; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The following disclosure provides many different embodiments or examples for implementing different features of the invention. To simplify the disclosure of the present invention, the components and settings of a specific example are described below. Furthermore, the present invention may repeat reference numerals and/or reference letters in the various examples, which have been repeated for purposes of simplicity and clarity and do not in themselves dictate a relationship between the various embodiments and/or configurations discussed.
The term "sequencing" as used in the embodiments of the present invention is also referred to as "sequencing" and "sequencing" as well as nucleic acid sequencing, including DNA sequencing and/or RNA sequencing, including long-fragment sequencing and/or short-fragment sequencing. The so-called "sequencing reaction" is the same as the sequencing reaction.
Referring to fig. 1, an embodiment of the invention provides a method for tracking sequencing consumables. During sequencing, the consumable is placed on a sequencing system, the sequencing system comprises an information reading and writing device and a prompting device, and the tracking method comprises the following steps:
s11, before the consumable is placed on the sequencing system and sequencing is carried out, reading information items and values of the information items of the consumable by using the information reading and writing device to obtain an information label of the consumable, wherein the information label of the consumable comprises the information items and the values of the information items of the consumable, the information items comprise at least one of types, detection items and identification bits, the values of the types comprise reaction devices and/or first reagents matched with the sequencing system, the values of the detection items comprise at least one of disease genetic detection items, and the values of the identification bits comprise used and unused;
s12, comparing the information label of the consumable with the standard information label of the consumable to obtain a comparison result, wherein the standard information label of the consumable comprises the information item of the consumable and the standard value of the information item, and the information item in the standard information label of the consumable covers the information item in the information label of the consumable;
s13, judging whether the consumable is normal according to the comparison result:
when the information label of the consumable is consistent with the standard information label of the consumable, S14, judging that the consumable is normal and prompting that the consumable is normal by using a prompting device; when the values of all corresponding information items in the information tag of the consumable part and the standard information tag of the consumable part are completely the same, the two information tags are considered to be consistent;
when the information label of the consumable is inconsistent with the standard information label of the consumable, S15, judging the consumable is abnormal and prompting the abnormality of the consumable by using a prompting device; and when the value of any corresponding information item in the information tag of the consumable part is different from that in the standard information tag of the consumable part, the information tag of the consumable part and the standard information tag of the consumable part are regarded as inconsistent.
According to the tracking method of the sequencing consumables, the information reading and writing device is used for reading the information tags of the consumables, and the prompting device is used for prompting the state of the consumables, so that the sequencing consumables are clear and traceable, sequencing result errors caused by artificial distinguishing or loading errors are avoided, the requirement for attributing the sequencing consumables in different types and/or different states of the same type is lowered, and the sequencing consumables can be simply and effectively realized.
Specifically, the sequencing system includes a support for holding the consumable, for example, when the consumable is a reaction device, the support includes a platform with a groove for holding the reaction device. The platform can be designed into a movable platform and is used for driving the reaction device to move in the sequencing process, so that the sequencing system can acquire/photograph information at different positions of the reaction device. The reaction device comprises a chip. When the consumable is a first reagent, the first reagent is generally contained in the first reagent cartridge, the support comprises a rack, the first reagent cartridge containing the first reagent is placed on the rack, and the sequencing system can draw the first reagent into the reaction device for a biochemical reaction during the testing process, in some examples, the biochemical reaction comprises an extension reaction, and/or a radical excision reaction. The extension reaction may be a ligation reaction and/or a synthesis reaction.
The information item includes at least one of a category, a detection item and an identification bit, and specifically, the information item includes the category, or the information item includes the category and the detection item, or the information item includes the category and the identification bit, or the information item includes the category, the detection item and the identification bit, or the information item includes the detection item and the identification bit.
In some embodiments, different information items in the information tags may be arranged in a set order, and information items in the standard information tags may also be arranged in the set order to facilitate comparison of the information items. For example, when the information item includes a category, a detection item, and an identification bit, the information item may be arranged in the information tag and the standard information tag in the category-detection item-identification bit order. In other embodiments, the information tags and the standard information tags may have different arrangement orders of the information items, and the same identifier may be added to the same information item, and when comparing, the values of the corresponding information item and the information item may be compared by determining whether the identifier is the same.
In some embodiments, it is preferable that the number of information items of the information tag is the same as the number of information items of the standard information tag.
In some embodiments, the values of the information items may be represented by different numbers, for example, the values of the categories may be represented by different two-digit numbers, the values of the detection items may be represented by different three-digit numbers, and the values of the identification bits may be represented by different one-digit numbers. In one example, an information tag is defined as: the type (00) detection item (001) is the mark bit (1), wherein, the value 00 of the type (00) represents a reaction device, the value 001 of the detection item (001) represents prenatal detection, and the value 1 of the mark bit (1) represents unused. In another example, an information tag is defined as: the type (01) detection item (010) indicates the bit (0), the value 01 of the type (01) indicates the first reagent, the value 010 of the detection item (010) indicates the tumor screening, and the value 0 of the identification bit (0) indicates that the reagent has been used.
The value of the test item is taken from the detection of at least one genetic disease, including but not limited to prenatal testing and tumor screening, and thus the tracking method of the embodiments of the present invention can be used for different genetic testing applications.
In embodiments of the present invention, different numbers of information items may be selected for comparison according to different sequencing requirements. For example, the information item of the consumable includes a category, a detection item and an identification bit and a corresponding value, and the standard information item of the consumable includes a category, a detection item and an identification bit and a corresponding standard value. In the first sequencing, only the value of the type of the consumable material needs to be compared with the standard value of the type of the consumable material to judge whether the consumable material is normal, and the sequencing result is not influenced without comparing the values of other two information items with the standard value. Obtaining a comparison result after the comparison, and judging whether the consumable is normal according to the comparison result, wherein the comparison result only contains the comparison between the type value of the consumable and the standard value of the type, so that when the type value of the consumable is completely the same as the standard value of the type, the information tag of the consumable is considered to be consistent with the standard information tag of the consumable; when the value of the type of the consumable is different from the standard value of the type, the information tag of the consumable is not consistent with the standard information tag of the consumable.
In the second sequencing, the value of the type of the consumable and the standard value of the type of the consumable need to be compared, and the value of the detection item of the consumable and the standard value of the detection item of the consumable need to judge whether the consumable is normal, and the sequencing result is not affected even if the value of the remaining information item and the standard value need not to be compared. Obtaining a comparison result after the comparison, and judging whether the consumable item is normal according to the comparison result, wherein the comparison result comprises the comparison of the value of the type of the consumable item and the standard value of the type of the consumable item, and the comparison of the value of the detection item of the consumable item and the standard value of the detection item, so that when the value of the type of the consumable item and the standard value of the type of the consumable item are completely the same, and when the value of the detection item of the consumable item and the standard value of the detection item are completely the same, the information tag of the consumable item is considered to be consistent with the standard information tag of the consumable item; and when the value of the type of the consumable is different from the standard value of the type and/or the value of the detection item of the consumable is different from the standard value of the detection item, the information tag of the consumable is not consistent with the standard information tag of the consumable.
Other situations can be understood with reference to the above. In summary, the comparison result can be obtained by determining the comparison quantity between the value of the information item of the consumable and the standard value of the information item according to actual needs. When the comparison result shows that the values of the compared information items of the consumables are completely the same as the standard values of all the corresponding information items, the information labels of the consumables are considered to be consistent with the standard information labels of the consumables; and when the comparison result shows that the value of the compared information item of the consumable is different from the standard value of any corresponding information item, the information label of the consumable is not consistent with the standard information label of the consumable.
The value and standard value of the information item are one of attributes, signs, parameters, symbols, and the like that distinguish the comparison object from the information item having comparability. For example, 00 and 01 in the case of the type (00) and the type (01) are parameters for distinguishing the reaction apparatus from the first reagent in the case of a comparable type. For another example, A, B under test items (a) and (B) is a symbol for distinguishing prenatal testing from tumor screening under comparable test items. The attributes, logos, parameters or symbols of the comparator can be customized to the particular comparator represented.
In some embodiments, the information item of the consumable and the value of the information item may be written in at the time of shipment of the consumable, forming an information tag of the consumable. The specific information item of the consumable and the value of the information item can be customized, so that the consumable is convenient for practical application.
The standard information tag of the consumable, which includes information items to be compared before sequencing, may be preset and stored before sequencing.
In some embodiments, the information item further includes a lot number of the consumable, an intra-lot water number, and a specification, the lot number of the consumable including a value of the lot number of the consumable, the intra-lot water number including a value of the intra-lot water number, and the specification including a value of the specification. In some embodiments, the value of the lot number of the consumable, the value of the lot flow number, and the value of the specification may all be represented by different numbers.
In some embodiments, the information reading and writing device comprises a Near Field Communication (NFC) card reader, and the consumable is attached with a tag card capable of reading near field communication, and the NFC card reader is close to the tag card so that the information reading and writing device can obtain the information tag of the consumable.
Therefore, the cost of the information reading and writing device is low, and the accuracy rate of information reading is high.
Specifically, the NFC card reader may be disposed near a support such as a platform or a rack on which the consumable is placed, so that when the consumable is placed on the support, the NFC card reader may read an information tag of the consumable on the tag card. Generally, the distance between the NFC card reader and the tag card is about 20mm, and the short distance can avoid reading other tag cards. For example, when the consumable is a first reagent, the first reagent is contained in a first reagent cartridge, and a label card is attached to the outer surface of the first reagent cartridge. Before sequencing, the plurality of first reagent kits are placed on the same support, and the NFC card reader is close to the label card, so that the condition that the NFC card reader reads information of the label card adjacent to the label card to be read can be avoided.
In some embodiments, the information tag of the consumable part includes a type and a type value, and the comparison result includes that the type value is not consistent with a standard value of the type in the standard information tag of the consumable part or the type value is consistent with a standard value of the type in the standard information tag of the consumable part;
when the value of the type is consistent with the standard value in the type of the standard information label of the consumable, judging that the consumable is normal;
and judging that the consumable is abnormal when the value of the category is not consistent with the standard value in the category of the standard information label of the consumable.
Therefore, consumables inconsistent with the demand types are prevented from being used in sequencing, and sequencing errors are further avoided.
Specifically, in the embodiment of the present invention, it is only necessary to compare the value of the type of the consumable material with the standard value of the type of the consumable material to determine whether the consumable material is normal. Before sequencing, can be used for placing the position setting information read write device of consumptive material at sequencing system in advance, and set for the standard information label of consumptive material, the value of the standard value of the kind of the type when making the standard information label of consumptive material in with correctly placing the consumptive material in the position is unanimous. When the consumable is placed on the sequencing system, the information reading and writing device obtains the value of the type of the consumable, and then compares the obtained value of the type with the standard value of the type in the standard information tag of the consumable to obtain a comparison result.
For example, the sequencing system comprises a platform for placing the reaction device and a bracket for placing a first kit, wherein the first kit contains a first reagent, a first information reading and writing device is arranged on the edge of the platform, and a second information reading and writing device is arranged on the edge of the bracket. In the standard information tag of the consumable part, the standard value of the type of the consumable part corresponding to the first information reading/writing device is 00, which indicates a reaction device, and the standard value of the type of the consumable part corresponding to the second information reading/writing device is 01, which indicates the first reagent.
In some embodiments, the information tag of the consumable part comprises the detection item and the value of the detection item, and the comparison result comprises that the value of the detection item is not consistent with the standard value of the detection item in the standard information tag of the consumable part or the value of the detection item is consistent with the standard value of the detection item in the standard information tag of the consumable part;
when the value of the detection item is consistent with the standard value of the detection item in the standard information tag of the consumable, judging that the consumable is normal;
and judging that the consumable is abnormal when the value of the detection item is inconsistent with the standard value of the detection item in the standard information label of the consumable.
Therefore, consumables inconsistent with the requirement detection project are prevented from being used in sequencing, and sequencing errors are further avoided.
Specifically, in the embodiment of the present invention, it is only necessary to compare the value of the detection item of the consumable material with the standard value of the detection item of the consumable material to determine whether the consumable material is normal. The standard information tag of the consumable can be set according to the sequencing purpose, for example, the detection item in the next sequencing is prenatal detection, then, before sequencing, the standard value of the detection item in the standard information tag of the consumable is set to 001, which represents prenatal detection, when the consumable is placed on the sequencing system, the information reading and writing device is utilized to obtain the value of the detection item of the information tag of the consumable, and then the value of the detection item of the consumable and the standard value 001 of the detection item in the standard information tag of the consumable are compared, so that the comparison result is obtained.
In some embodiments, the information tag of the consumable comprises the identification bits and the values of the identification bits, and the comparison result comprises that the values of the identification bits are not consistent with the standard values of the identification bits in the standard information tag of the consumable or the values of the identification bits are consistent with the standard values of the identification bits in the standard information tag of the consumable;
when the value of the identification position is consistent with the standard value of the identification position in the standard information tag of the consumable, judging that the consumable is normal;
and when the value of the identification bit is inconsistent with the standard value of the identification bit in the standard information tag of the consumable, judging that the consumable is abnormal.
Thus, the used consumables are prevented from being used again, and sequencing errors are further avoided.
Specifically, in the embodiment of the present invention, it is only necessary to compare the value of the identification bit of the consumable with the standard value of the identification bit of the consumable to determine whether the consumable is normal. The standard value of the marker bit in the standard information tag of the consumable may be set to unused prior to sequencing. Before sequencing, if the value of the identification position of the consumable arranged on the sequencing system is used, after the information tag of the consumable is obtained by using the information reading and writing device, when the information tag of the consumable is compared with the standard information tag of the consumable, the value of the identification position of the consumable is inconsistent with the standard value of the identification position in the standard information tag of the consumable, and the consumable is judged to be abnormal; if the value of the identification bit of the consumable on the sequencing system is unused, then, after the information tag of the consumable is obtained by utilizing the information reading and writing device, when the information tag of the consumable is compared with the standard information tag of the consumable, the value of the identification bit of the consumable is consistent with the standard value of the identification bit in the standard information tag of the consumable, and the consumable is judged to be normal.
For example, the standard value of the flag in the standard information tag of the consumable material is 1 (indicating unused), and before sequencing, if the value of the flag of the consumable material placed on the sequencing system is 0 (indicating used), the consumable material is determined to be abnormal; if the value of the identification bit of the consumable placed on the sequencing system is 1 (indicating that the consumable is not used), the consumable is judged to be normal.
In certain embodiments, the value of the species comprises a second reagent and/or a third reagent that is matched to the sequencing system.
Therefore, the application range of the tracking method of the sequencing consumables can be expanded.
Specifically, in one example, the first reagent is a sequencing reagent, the second reagent is a group excision reagent, and the third reagent is an imaging reagent, that is, in different sequencing stages, when different reagents are extracted, whether different reagents are suitable for the current sequencing stage can be judged, and sequencing errors are avoided.
In some embodiments, the information tag of the consumable comprises an identification bit and a value of the identification bit, and after the sequencing is finished, the value of the identification bit is written into the information reading and writing device to be used to cover the original value of the identification bit.
In this manner, the value of the identification bits of the consumable may be altered such that the consumable that has been used will not be used again.
For example, before sequencing, the value of the flag bit of the consumable part placed on the sequencing system is 1 (indicating unused), and after sequencing is finished, the information reading/writing device writes 0 to cover 1, so that the value of the flag bit of the consumable part is changed from 1 (unused) to 0 (used).
In some embodiments, the information item includes an expiration date and a value of the expiration date of the consumable, the method including: obtaining a value of a current date and comparing the value of the current date with a value of an effective date before the consumable is placed on a sequencing system and sequenced;
the comparison result comprises a value that the value of the current date does not exceed the effective date or a value that the value of the current date exceeds the effective date;
when the value of the current date does not exceed the value of the effective date, judging that the consumable is normal;
and when the value of the current date exceeds the value of the effective date, judging that the consumable is abnormal.
Therefore, overdue consumables are prevented from being used for sequencing, and sequencing errors are further avoided.
In particular, the value of the current date may be determined from the value of the system date of the sequencing system. In some embodiments, the value of the date may be expressed as YYMMDD, where YY represents year, MM represents month, and DD represents day. In one example, the current date value is 171231, which indicates No. 12/31 in 2017, and if the expiration date value of the consumable part is 171130, which indicates No. 11/30 in 2017, the current date value exceeds the expiration date value, and it is determined that the consumable part is abnormal; if the effective date value of the consumable supplies is 180331 and represents No. 03/31 in 2018, the current date value does not exceed the effective date value, and the consumable supplies are judged to be normal.
In some embodiments, the information items include a delivery date, an expiration date, a delivery date value, and an expiration date value of the consumable, and the method includes: comparing the value of the effective date with the value of the factory date;
the comparison result comprises a value that the value of the factory date does not exceed the effective date or a value that the value of the factory date exceeds the effective date;
when the value of the delivery date does not exceed the value of the effective date, judging that the consumable is normal;
and when the value of the delivery date exceeds the value of the effective date, judging that the consumable is abnormal.
Therefore, the probability increase of sequencing errors caused by date entry errors of the early consumables can be avoided.
Specifically, normally speaking, the value of the delivery date of the consumable will not exceed the value of the expiration date of the consumable, but if in the production process of the consumable, the date entry of the consumable itself has errors, so that the value of the delivery date of the consumable exceeds the value of the expiration date of the consumable, and then under the condition that the date entry of the consumable itself has errors, the probability that other information of the consumable has errors is greatly improved, and further the probability of sequencing errors is increased. Therefore, before sequencing, the consumables with wrong date entry are screened out, and the probability of sequencing errors can be reduced.
In some embodiments, the information item comprises reserved bits and values of the reserved bits, the method comprising:
and receiving a new information item to be written and the value of the new information item, and respectively writing the values of the new information item and the new information item into the values of the reserved bits and the reserved bits by using an information reading and writing device.
Therefore, information expansion can be conveniently carried out on the information tag of the consumable.
Specifically, in some embodiments, the new information item and the value of the new information item may be input by a user in a human-computer interaction interface of the sequencing system, and the new information item and the value of the new information item are written to the values of the reserved bits and the reserved bits, respectively, by using the information reading and writing device.
In some embodiments, the prompting device issues a visual and/or audible prompt when prompted by the prompting device.
Therefore, the visual prompt message and/or the auditory prompt message can be easily received by the user, and the prompt success rate is improved.
In particular, the prompting device may include a display screen and/or a speaker, and the display screen of the prompting device may be controlled to display visual prompting information when prompting is performed, e.g., the display screen displays "abnormal", "! The prompt device comprises a loudspeaker and a display device, wherein the loudspeaker is used for displaying text information of abnormal consumables, or displaying text information of normal consumables, OK, normal consumables, OK and the like, and/or controlling the loudspeaker of the prompt device to send auditory prompt information, for example, the loudspeaker sends sound information of abnormal consumables, or sends sound information of normal consumables, OK and the like. In other embodiments, the audible prompting message also includes sound generated by vibration caused by the prompting device, for example, the prompting device includes a vibration motor, and when the consumable is normal, the vibration motor is controlled to generate a plurality of vibrations at short intervals; when the consumables are abnormal, the vibration motor is controlled to vibrate for multiple times at longer intervals.
In some embodiments, the value of the information item is an encrypted value, the method comprising: the read value of the information item is decrypted to compare the information tag of the consumable with the standard information tag of the consumable. Thus, the value of the information item can be prevented from being easily changed by human interference.
Specifically, the encryption and decryption algorithms may employ commonly used encryption and decryption algorithms.
Referring to FIG. 2, the present invention provides a sequencing system 100 for tracking sequencing consumables. During sequencing, the consumable is placed on the sequencing system 100, the sequencing system 100 comprises a control device 102, an information reading and writing device 104 and a prompting device 106, the control device 102 is connected with the information reading and writing device 104 and the prompting device 106, before the consumable is placed on the sequencing system 100 and sequencing is performed, the control device 102 is used for reading information items and values of the information items of the consumable by using the information reading and writing device 104 to obtain an information tag of the consumable, the information tag of the consumable comprises the information items and the values of the information items of the consumable, the information items comprise at least one of types, detection items and identification bits, the values of the types comprise reaction devices and/or first reagents matched with the sequencing system, the values of the detection items comprise at least one of disease genetic detection items, and the values of the identification bits comprise used and unused;
the control device 102 is configured to compare the information tag of the consumable with a standard information tag of the consumable to obtain a comparison result, where the standard information tag of the consumable includes an information item of the consumable and a standard value of the information item, and the information item in the standard information tag of the consumable covers the information item in the information tag of the consumable;
judging whether the consumables are normal or not according to the comparison result:
when the information tag of the consumable is consistent with the standard information tag of the consumable, judging that the consumable is normal and prompting that the consumable is normal by utilizing the prompting device 106; when the values of all corresponding information items in the information tag of the consumable part and the standard information tag of the consumable part are completely the same, the two information tags are considered to be consistent;
when the information tag of the consumable is inconsistent with the standard information tag of the consumable, judging that the consumable is abnormal and prompting the abnormality of the consumable by utilizing the prompting device 106; and when the value of any corresponding information item in the information tag of the consumable part is different from that in the standard information tag of the consumable part, the information tag of the consumable part and the standard information tag of the consumable part are regarded as inconsistent.
It should be noted that the explanation and description of the technical features and advantages of the tracking method for sequencing consumables in any of the above embodiments and examples are also applicable to the sequencing system 100 of the present embodiment, and are not detailed herein to avoid redundancy.
In addition, the control device 102 may include a personal computer, the personal computer is connected to the information reading/writing device 104 and the prompting device 106, and the prompting device 106 may be integrated into the personal computer. The personal computer may be installed with software that controls the sequencing process.
In some embodiments, the information reading/writing device 104 includes a near field communication card reader, and a tag card readable by near field communication is attached to the consumable, and the near field communication card reader is close to the tag card so that the information reading/writing device can obtain the information tag of the consumable.
In some embodiments, the information tag of the consumable part includes a type and a type value, and the comparison result includes that the type value is not consistent with a standard value of the type in the standard information tag of the consumable part or the type value is consistent with a standard value of the type in the standard information tag of the consumable part;
the control device 102 is used for judging that the consumable is normal when the value of the type is consistent with the standard value of the type in the standard information label of the consumable;
the control device 102 is used for judging the consumable material is abnormal when the value of the type is not consistent with the standard value of the type in the standard information label of the consumable material.
In some embodiments, the information tag of the consumable part comprises the detection item and the value of the detection item, and the comparison result comprises that the value of the detection item is not consistent with the standard value of the detection item in the standard information tag of the consumable part or the value of the detection item is consistent with the standard value of the detection item in the standard information tag of the consumable part;
the control device 102 is used for judging that the consumable is normal when the value of the detection item is consistent with the standard value of the detection item in the standard information label of the consumable;
the control device 102 is used for judging the consumable material is abnormal when the value of the detection item is not consistent with the standard value of the detection item in the standard information label of the consumable material.
In some embodiments, the information tag of the consumable comprises the identification bits and the values of the identification bits, and the comparison result comprises that the values of the identification bits are not consistent with the standard values of the identification bits in the standard information tag of the consumable or the values of the identification bits are consistent with the standard values of the identification bits in the standard information tag of the consumable;
the control device 102 is used for judging that the consumable is normal when the value of the identification bit is consistent with the standard value of the identification bit in the standard information tag of the consumable;
the control device 102 is used for judging the consumable material is abnormal when the value of the identification bit is not consistent with the standard value of the identification bit in the standard information label of the consumable material.
In certain embodiments, the value of the species comprises a second reagent and/or a third reagent that is matched to the sequencing system.
In some embodiments, the information tag of the consumable comprises an identification bit and a value of the identification bit, and the control device 102 is configured to write the value of the identification bit to a value used to overwrite the original value of the identification bit by using the information reading/writing device 104 after the sequencing is completed.
In some embodiments, the information items comprise an expiration date and a value of the expiration date of the consumable, the control device being configured to obtain the value of the current date and compare the value of the current date with the value of the expiration date before the consumable has been placed on the sequencing system 100 and sequenced;
the comparison result comprises a value that the value of the current date does not exceed the effective date or a value that the value of the current date exceeds the effective date;
the control device 102 is used for judging whether the consumable is normal when the value of the current date does not exceed the value of the effective date;
the control device 102 is used for judging the consumable material is abnormal when the value of the current date exceeds the value of the effective date.
In some embodiments, the information item comprises a release date, an expiration date, a value of the release date, and a value of the expiration date of the consumable, the control device being configured to compare the value of the expiration date with the value of the release date;
the comparison result comprises a value that the value of the factory date does not exceed the effective date or a value that the value of the factory date exceeds the effective date;
the control device 102 is used for judging that the consumables are normal when the value of the factory date does not exceed the value of the effective date;
the control device 102 is used for judging that the consumable material is abnormal when the value of the factory date exceeds the value of the effective date.
In some embodiments, the information item includes reserved bits and values of the reserved bits, and the control device 102 is configured to receive a new information item to be written and values of the new information item and write the new information item and the values of the new information item to the values of the reserved bits and the reserved bits, respectively, by using the information reading and writing device 104.
In some embodiments, the control device 102 is configured to control the prompting device 106 to issue visual and/or audible prompting information when prompted by the prompting device 106.
In some embodiments, the value of the information item is an encrypted value, and the control device 102 is configured to decrypt the read value of the information item to compare the information tag of the consumable with the standard information tag of the consumable.
Referring to fig. 2, an embodiment of the present invention provides a control device 102 for tracking sequencing consumables in a sequencing system 100, where the sequencing system 100 includes an information reading/writing device 104 and a prompting device 106, and the control device 102 includes:
a storage unit 202 for storing data, the data comprising computer executable programs;
a processor 204 for executing a computer-executable program, the executing of the computer-executable program comprising performing the method of any of the above embodiments.
A computer-readable storage medium of an embodiment of the present invention stores a program for execution by a computer, and executing the program includes performing the method of any of the above embodiments. Computer-readable storage media may include, but are not limited to: read-only memory, random access memory, magnetic or optical disk, and the like.
In the description herein, references to the description of the terms "one embodiment," "certain embodiments," "an illustrative embodiment," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The logic and/or steps represented in the flowcharts or otherwise described herein, such as an ordered listing of executable instructions that can be considered to implement logical functions, can be embodied in any computer-readable storage medium for use by or in connection with an instruction execution system, apparatus, or device, such as a computer-based system, processor-containing system, or other system that can fetch the instructions from the instruction execution system, apparatus, or device and execute the instructions.
In addition, each functional unit in the embodiments of the present invention may be integrated into one processing module, or each unit may exist alone physically, or two or more units are integrated into one module. The integrated module can be realized in a hardware mode, and can also be realized in a software functional module mode. The integrated module, if implemented in the form of a software functional module and sold or used as a stand-alone product, may also be stored in a computer readable storage medium.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and not to be construed as limiting the present invention, and those skilled in the art can make changes, modifications, substitutions and alterations to the above embodiments within the scope of the present invention.

Claims (25)

1. A tracking method of sequencing consumables is provided, during sequencing, the consumables are placed on a sequencing system, the sequencing system comprises an information reading and writing device and a prompting device, and the tracking method is characterized by comprising the following steps:
before the consumable is placed on the sequencing system and sequencing is carried out, reading an information item of the consumable and a value of the information item by using the information reading and writing device to obtain an information tag of the consumable, wherein the information tag of the consumable comprises the information item of the consumable and the value of the information item, the information item comprises at least one of a category, a detection item and an identification bit, the value of the category comprises a reaction device and/or a first reagent matched with the sequencing system, the value of the detection item comprises at least one of a disease genetic detection item, and the value of the identification bit comprises used and unused;
comparing the information tag of the consumable with a standard information tag of the consumable to obtain a comparison result, wherein the standard information tag of the consumable comprises an information item of the consumable and a standard value of the information item, and the information item in the standard information tag of the consumable covers the information item in the information tag of the consumable;
according to the comparison result, judging whether the consumable is normal:
when the information label of the consumable is consistent with the standard information label of the consumable, judging that the consumable is normal and prompting that the consumable is normal by using the prompting device; when the values of all corresponding information items in the information tag of the consumable part and the standard information tag of the consumable part are completely the same, the information tag of the consumable part and the standard information tag of the consumable part are regarded as consistent;
when the information tag of the consumable is inconsistent with the standard information tag of the consumable, judging that the consumable is abnormal and prompting the abnormality of the consumable by using the prompting device; and when the value of any corresponding information item in the information tag of the consumable part and the value of any corresponding information item in the standard information tag of the consumable part are different, the information tag of the consumable part and the standard information tag of the consumable part are regarded as inconsistent.
2. The method of claim 1, wherein the information reading and writing device comprises a near field communication card reader, and a tag card readable by near field communication is attached to the consumable, and the near field communication card reader is close to the tag card so that the information reading and writing device can obtain the information tag of the consumable.
3. The method according to claim 1, wherein the information tag of the consumable includes the category and a value of the category, and the comparison result includes that the value of the category does not coincide with a standard value of the category in the standard information tag of the consumable or that the value of the category coincides with a standard value of the category in the standard information tag of the consumable;
when the value of the category is consistent with the standard value of the category in the standard information tag of the consumable, judging that the consumable is normal;
and judging that the consumable item is abnormal when the value of the category is not consistent with the standard value of the category in the standard information label of the consumable item.
4. The method of claim 1, wherein the information tag of the consumable includes the item under test and a value of the item under test, and the comparison result includes that the value of the item under test does not coincide with a standard value of the item under test in the standard information tag of the consumable or the value of the item under test coincides with a standard value of the item under test in the standard information tag of the consumable;
when the value of the detection item is consistent with the standard value of the detection item in the standard information label of the consumable, judging that the consumable is normal;
and when the value of the detection item is not consistent with the standard value of the detection item in the standard information label of the consumable, judging that the consumable is abnormal.
5. The method according to any one of claims 1 to 4, wherein the information tag of the consumable comprises the identification bits and the values of the identification bits, and the comparison result comprises that the values of the identification bits do not coincide with the standard values of the identification bits in the standard information tag of the consumable or that the values of the identification bits coincide with the standard values of the identification bits in the standard information tag of the consumable;
when the value of the identification position is consistent with the standard value of the identification position in the standard information tag of the consumable, judging that the consumable is normal;
and when the value of the identification bit is not consistent with the standard value of the identification bit in the standard information tag of the consumable, judging that the consumable is abnormal.
6. The method of claim 1, wherein the value of the species comprises a second reagent and/or a third reagent that matches the sequencing system.
7. The method according to claim 1, wherein the information tag of the consumable comprises the identification bit and the value of the identification bit, and after the sequencing is finished, the value of the identification bit is written to the value used to cover the original value of the identification bit by using the information reading and writing device.
8. The method of claim 1, wherein the information item includes an expiration date of the consumable and a value of the expiration date, the method comprising: obtaining a current date value and comparing the current date value to the expiration date value before the consumable has been placed on the sequencing system and the sequencing is performed;
the comparison result comprises the value of the current date not exceeding the value of the effective date or the value of the current date exceeding the value of the effective date;
when the value of the current date does not exceed the value of the effective date, judging that the consumable is normal;
and when the value of the current date exceeds the value of the effective date, judging that the consumable is abnormal.
9. The method of claim 1, wherein the information items include a factory date, an expiration date, a value of the factory date, and a value of the expiration date of the consumable, the method comprising: comparing the value of the effective date with the value of the factory date;
the comparison result comprises a value that the value of the factory date does not exceed the effective date or a value that the value of the factory date exceeds the effective date;
when the value of the delivery date does not exceed the value of the effective date, judging that the consumable is normal;
and when the value of the delivery date exceeds the value of the effective date, judging that the consumable is abnormal.
10. The method of claim 1, wherein the information item includes reserved bits and values of the reserved bits, the method comprising:
and receiving a new information item to be written and the value of the new information item, and respectively writing the values of the new information item and the new information item into the reserved bit and the value of the reserved bit by using the information reading and writing device.
11. The method of claim 1, wherein the prompting device issues a visual prompt and/or an audible prompt when prompted by the prompting device.
12. The method of claim 1, wherein the value of the information item is an encrypted value, the method comprising: decrypting the read value of the information item to compare the information tag of the consumable with the standard information tag of the consumable.
13. A sequencing system is used for tracking sequencing consumables, the consumables are placed on the sequencing system during sequencing, the sequencing system is characterized by comprising a control device, an information reading and writing device and a prompting device, the control device is connected with the information reading and writing device and the prompting device, before the consumables are placed on the sequencing system and sequencing is carried out, the control device is used for reading information items of the consumables and values of the information items by using the information reading and writing device to obtain information labels of the consumables, the information labels of the consumables comprise the information items of the consumables and the values of the information items, the information items comprise at least one of types, detection items and identification bits, the values of the types comprise a reaction device and/or a first reagent matched with the sequencing system, and the values of the detection items comprise at least one of disease genetic detection items, the value of the identification bit comprises used and unused;
the control device is used for comparing the information tag of the consumable with the standard information tag of the consumable to obtain a comparison result, the standard information tag of the consumable comprises the information item of the consumable and the standard value of the information item, and the information item in the standard information tag of the consumable covers the information item in the information tag of the consumable;
according to the comparison result, judging whether the consumable is normal:
when the information label of the consumable is consistent with the standard information label of the consumable, judging that the consumable is normal and prompting that the consumable is normal by using the prompting device; when the values of all corresponding information items in the information tag of the consumable part and the standard information tag of the consumable part are completely the same, the information tag of the consumable part and the standard information tag of the consumable part are regarded as consistent;
when the information tag of the consumable is inconsistent with the standard information tag of the consumable, judging that the consumable is abnormal and prompting the abnormality of the consumable by using the prompting device; and when the value of any corresponding information item in the information tag of the consumable part and the value of any corresponding information item in the standard information tag of the consumable part are different, the information tag of the consumable part and the standard information tag of the consumable part are regarded as inconsistent.
14. The system of claim 13, wherein the information reading and writing device comprises a near field communication card reader, and a tag card readable by near field communication is attached to the consumable, and the near field communication card reader is close to the tag card so that the information reading and writing device can obtain the information tag of the consumable.
15. The system according to claim 13, wherein the information tag of the consumable includes the category and a value of the category, and the comparison result includes that the value of the category does not coincide with a standard value of the category in the standard information tag of the consumable or that the value of the category coincides with a standard value of the category in the standard information tag of the consumable;
the control device is used for judging that the consumable is normal when the value of the type is consistent with the standard value of the type in the standard information tag of the consumable;
and the control device is used for judging that the consumable is abnormal when the value of the type is not consistent with the standard value of the type in the standard information label of the consumable.
16. The system of claim 13, wherein the information tag of the consumable includes the item under test and a value of the item under test, and the comparison result includes that the value of the item under test does not coincide with a standard value of the item under test in the standard information tag of the consumable or the value of the item under test coincides with a standard value of the item under test in the standard information tag of the consumable;
the control device is used for judging that the consumable is normal when the value of the detection item is consistent with the standard value of the detection item in the standard information label of the consumable;
and the control device is used for judging that the consumable is abnormal when the value of the detection item is not consistent with the standard value of the detection item in the standard information label of the consumable.
17. The system according to any one of claims 13 to 16, wherein the information tag of the consumable comprises the identification bits and the values of the identification bits, and the comparison result comprises that the values of the identification bits do not coincide with the standard values of the identification bits in the standard information tag of the consumable or that the values of the identification bits coincide with the standard values of the identification bits in the standard information tag of the consumable;
the control device is used for judging that the consumable is normal when the value of the identification bit is consistent with the standard value of the identification bit in the standard information tag of the consumable;
and the control device is used for judging that the consumable is abnormal when the value of the identification bit is not consistent with the standard value of the identification bit in the standard information tag of the consumable.
18. The system of claim 13, wherein the value of the species comprises a second reagent and/or a third reagent that matches the sequencing system.
19. The system of claim 13, wherein the information tag of the consumable comprises the identification bit and a value of the identification bit, and the control device is configured to write the value of the identification bit to a value that is used to overwrite the original value of the identification bit by using the information reading and writing device after the sequencing is completed.
20. The system of claim 13, wherein said items of information include an expiration date of said consumable and a value of said expiration date, said control means being adapted to, before said consumable has been placed on said sequencing system and said sequencing is performed, obtain a value of a current date and compare said value of said current date with said value of said expiration date;
the comparison result comprises the value of the current date not exceeding the value of the effective date or the value of the current date exceeding the value of the effective date;
the control device is used for judging that the consumable is normal when the value of the current date does not exceed the value of the effective date;
and the control device is used for judging that the consumable is abnormal when the value of the current date exceeds the value of the effective date.
21. The system according to claim 13, wherein the information items include a delivery date, an expiration date, a value of the delivery date, and a value of the expiration date of the consumable, the control means being configured to compare the value of the expiration date with the value of the delivery date;
the comparison result comprises a value that the value of the factory date does not exceed the effective date or a value that the value of the factory date exceeds the effective date;
the control device is used for judging that the consumable is normal when the value of the delivery date does not exceed the value of the effective date;
and the control device is used for judging that the consumable is abnormal when the value of the factory date exceeds the value of the effective date.
22. The system of claim 13, wherein the information items include reserved bits and values of the reserved bits, and the control means is configured to receive a new information item to be written and values of the new information item and to write the new information item and the values of the new information item to the values of the reserved bits and the reserved bits, respectively, using the information reading and writing means.
23. The system of claim 13, wherein the control device is configured to control the prompting device to issue a visual prompt and/or an audible prompt when prompted by the prompting device.
24. The system according to claim 13, wherein the value of the item of information is an encrypted value, the control means being adapted to decrypt the read value of the item of information to compare the information tag of the consumable with a standard information tag of the consumable.
25. A control device for tracking sequencing consumables on a sequencing system, wherein the sequencing system comprises an information reading and writing device and a prompting device, and the control device comprises:
a storage unit for storing data, the data comprising a computer executable program;
a processor for executing the computer-executable program, execution of the computer-executable program comprising performing the method of any of claims 1-12.
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