CN107049868B

CN107049868B - Disinfecting detergent for pets and preparation method thereof

Info

Publication number: CN107049868B
Application number: CN201710304558.2A
Authority: CN
Inventors: 梁剑平; 刘宇; 郝宝成; 王学红; 陶蕾; 郭文柱; 尚若锋; 郭志廷; 赵凤舞; 杨珍; 陈虹; 贾忠; 于鹏; 张新刚
Original assignee: Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
Current assignee: HENAN HUA MU BIOLOGICAL TECHNOLOGY Co.,Ltd.
Priority date: 2017-05-03
Filing date: 2017-05-03
Publication date: 2020-06-05
Anticipated expiration: 2037-05-03
Also published as: CN107049868A

Abstract

The invention discloses a pet disinfectant detergent which is prepared from the following raw materials, by weight, 15 parts of fatty alcohol-polyoxyethylene ether sodium sulfate, 5-9 parts of alkyl glycoside, 1-5 parts of α -sodium alkenyl sulfonate, 0.8 part of sodium hydroxide, 15 parts of an antibacterial component and 59.2 parts of water, and provides a preparation method of the detergent.

Description

Disinfecting detergent for pets and preparation method thereof

Technical Field

The invention belongs to the technical field of veterinary medicines, and particularly relates to a disinfectant detergent for pets and a preparation method thereof.

Background

With the improvement of urban living standard, the housing condition is continuously improved, the composition of family population is continuously changed, more and more families are provided for raising pet such as cat and dog, the life of pet and human is close, and the health condition is increasingly paid attention by pet raising owners. The pet breeding owner is troubled by pet diseases such as dermatitis, pain and itch, pathological depilation and the like. The fur of the pet is infected with various pathogenic bacteria to carry, and the pathogenic bacteria threaten the health of the pet keeper more directly.

Detergents and disinfectants are indispensable commodities in pet breeding and bear important duties of keeping the environment clean and sanitary and being healthy and safe for human and animals. Along with the gradual improvement of the health consciousness of people, new requirements on detergents and disinfectants of pets are also put forward. At present, the detergent and the disinfectant specially used for pets are less, and the following defects mainly exist: 1. the product has single function and can not meet the dual requirements of washing and disinfection; 2. the synthetic bactericides and the plant-source bactericides cannot be clearly positioned and are unreasonable to use. Aiming at the defects, the invention designs and researches an antibacterial detergent according to the market demand and the characteristics of the use environment.

Disclosure of Invention

The first object of the present invention is to solve the above problems and to provide a disinfectant detergent for pets, wherein the antibacterial components in the disinfectant detergent are selected from natural plants, and the disinfectant detergent is compounded with a detergent, and has the effects of sterilizing, disinfecting and caring animal fur.

The second purpose of the invention is to provide a preparation method of the detergent.

The disinfectant detergent for pets is prepared from the following raw materials in parts by weight:

15 parts of sodium fatty alcohol-polyoxyethylene ether sulfate (AES), 5-9 parts of alkyl glycoside (APG), 1-5 parts of α -sodium alkenyl sulfonate (AOS), 0.8 part of sodium hydroxide, 15 parts of antibacterial component and 59.2 parts of water.

Preferably, the detergent is prepared from the following raw materials in parts by weight:

15 parts of sodium fatty alcohol-polyoxyethylene ether sulfate (AES), 8 parts of alkyl glycoside (APG), 2 parts of α -sodium alkenyl sulfonate (AOS), 0.8 part of sodium hydroxide, 15 parts of an antibacterial component and 59.2 parts of water.

Further, the antibacterial component is prepared from the following raw materials in parts by weight:

1-10 parts of dodder volatile oil, 1-10 parts of salvia extract, 1-10 parts of aloperine, 1-10 parts of ormosine and 5 parts of 90% ethanol.

Preferably, the antibacterial component is prepared from the following raw materials in parts by weight:

2 parts of dodder volatile oil, 3 parts of salvia extract, 3 parts of aloperine, 2 parts of ormosine and 5 parts of 90% ethanol.

The preparation method of the disinfectant detergent for pets comprises the following steps:

(1) extracting the aerial parts of the aired pelargonium odoratissimum by adopting a water vapor extraction method, collecting distillate, and extracting and drying the distillate to obtain the volatile oil of the pelargonium odoratissimum;

(2) adding the essential oil of the Bumper with the Salvia miltiorrhiza Bunge, the aloperine and the ormosine obtained in the step (1) into 90% ethanol, heating and stirring to dissolve the essential oil, and cooling to obtain an antibacterial component;

(3) and (3) adding sodium fatty alcohol polyoxyethylene ether sulfate (AES) into water with the temperature of 45 ℃, stirring at constant temperature until the sodium fatty alcohol polyoxyethylene ether sulfate (AES) is dissolved, then adding alkyl glycoside (APG), α -sodium alkenyl sulfonate (AOS), sodium hydroxide and the antibacterial component obtained in the step (2), and stirring until the components are completely dissolved to obtain the disinfectant detergent for pets.

Further, in the step (1), the water vapor extraction time is 4 hours, the extraction solvent is petroleum ether, and anhydrous sodium sulfate is selected as a drying agent for drying treatment.

Further, in the step (2), the heating temperature is 35-37 ℃.

The disinfectant detergent for pets is applied to pet cleaning.

The special disinfectant detergent for pets has strict requirements on the safety of human and animal bodies, and the bactericide is suitable to be selected from natural plants. The botanical fungicide applied to the pet special disinfectant detergent needs to meet the following conditions: firstly, the solubility in water and a surfactant is good, so that precipitation or layering cannot be caused; secondly, the odor is mild, and after washing, pungent odor cannot be left on the surface of the fur, so that the germicides of the allicin and the cinnamaldehyde are not applicable; then, the acid-base stability is good, and the bacteriostatic requirement is met within a proper addition amount. In view of the above conditions, the antibacterial component in the disinfectant detergent for pets is prepared by compounding the essential oil of the dodder, the extract of the salvia miltiorrhiza bunge, the aloperine and the ormosidine.

The Bumper-top incense is a plant of Geranium of Geraniaceae, has the advantages of heat resistance, cold resistance and sunshine preference in Europe and southwest Asia areas, can emit comfortable fragrance after being touched, has the beauty of the Bumper-top incense, has the smell like mint, slightly takes apple flavor, and has the effects of refreshing, clearing away heat, relieving summer heat, repelling mosquitoes, diminishing inflammation, diminishing swelling and caring skin. The essential component of the dodder volatile oil is a monoterpene compound, the relative percentage content of the dodder volatile oil accounts for 92 percent of the total volatile oil, the dodder volatile oil has strong antibacterial and bactericidal effects, and the higher the concentration of the volatile oil is, the stronger the antibacterial effect is.

The main antibacterial components in Saviae Miltiorrhizae radix extract are cryptotanshinone and dihydrotanshinone, and have inhibitory effect on in vitro Staphylococcus, Escherichia coli and Bacillus proteus.

The aloperine mainly exists in aloperine plants and has broad-spectrum antibacterial property.

The abrine is mainly present in herba Ormosiae Hosiei, and has antibacterial effect.

The special detergent for the pets mainly aims at cleaning the animals, and needs to have good affinity, nonirritant and the like according to the characteristics of fur of the animals.

The sodium fatty alcohol polyoxyethylene ether sulfate (AES) is an anionic surfactant, and has excellent decontamination, emulsification and foaming performances and more importantly has hard water resistance in the aspect of washing efficacy. In addition, the detergent can be compounded with other various surfactants for use, so that the detergency is improved. From the aspect of safety and environmental protection, the AES has mild property, does not damage the skin, has 99 percent of biodegradation degree and is a green and environment-friendly surfactant.

The alkyl glycoside (APG) is a nonionic surfactant and has good emulsifying and foaming properties. On one hand, the cleaning agent has good compounding property, generates a synergistic effect after being compounded with AES, and can obviously improve the decontamination effect. On the other hand, the detergent has good compatibility with plastic products, and does not cause cracking of the plastic products after washing. In addition, APG is low in skin irritation and non-toxic.

α -sodium alkenyl sulfonate (AOS) belongs to an anionic surfactant, has very good mildness which is close to a nonionic surfactant, has excellent foamability and foam stability, has good compatibility with other surfactants, especially has a synergistic effect with AES in a certain proportion, and has good decontamination effect.

The invention has the beneficial effects that:

the pet disinfectant detergent provided by the invention is prepared from natural antibacterial components and detergent components, has the functions of disinfecting and cleaning pet fur, and is prepared by compounding natural plants and extracts thereof. The detergent has mild smell and small irritation to pets and people, can greatly reduce the bacteria carrying amount in pet fur, ensure the health of pets, prevent diseases, has therapeutic effect on dermatitis, phlegm itch, pathological depilation and other diseases caused by infection, and overcomes the defect that the existing shampoo bath products only have cleaning effect.

Detailed Description

The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and description, and is in no way intended to limit the invention.

Example 1

Method for preparing antibacterial component

(1) Air drying aerial parts of fresh Bumper-top-bark incense, pulverizing, weighing 100g of coarse powder, extracting volatile oil by steam distillation for 4h, extracting distillate with petroleum ether to obtain light yellow liquid, drying with anhydrous sodium sulfate to obtain Bumper-top-bark incense volatile oil, and storing in a refrigerator for later use.

(2) Accurately weighing the following substances in parts by weight: 1-10 parts of dodder volatile oil, 1-10 parts of salvia miltiorrhiza, 1-10 parts of aloperine and 1-10 parts of ormosine.

(3) Adding the above materials into 5 parts of 90% ethanol, heating to 35-37 deg.C, stirring to dissolve completely, and cooling to obtain antibacterial component.

Example 2

Preparation method of disinfectant detergent for pets

Adding 15 parts by weight of fatty alcohol-polyoxyethylene ether sodium sulfate (AES) into 59.2 parts by weight of deionized water at 45 ℃, stirring at a constant temperature until the mixture is dissolved, then adding 5-9 parts by weight of alkyl glycoside (APG), 1-5 parts by weight of α -alkenyl sodium sulfonate (AOS), 0.8 part by weight of sodium hydroxide and 15 parts by weight of the antibacterial component in the embodiment 1, and stirring until the mixture is completely dissolved to obtain the disinfectant detergent for pets.

Next, the screening of antibacterial components, the decontamination test and the disinfection effect test of the pet disinfectant detergent were performed.

First, screening of antibacterial formula

The single pelargonium odoratissimum volatile oil has strong inhibition resistance to escherichia coli and staphylococcus aureus. The extracts of plants such as salvia miltiorrhiza, sophora alopecuroides, ormosia and the like have weak bacteriostatic ability, so that the extracts need to be compounded in use. The proper mass ratio of the salvia miltiorrhiza extract to the aloperine has the synergistic bacteriostatic action on escherichia coli and staphylococcus aureus; similarly, the appropriate mass ratio of the salvia miltiorrhiza extract to the ormosine has a synergistic bacteriostatic action on escherichia coli and staphylococcus aureus; the proper mass ratio of the ormosine to the aloperine has the synergistic bacteriostatic action on escherichia coli and staphylococcus aureus.

1.1 test materials

The test plants: the Bumper incense is purchased from the flower market in Lanzhou city of Gansu province;

salvia miltiorrhiza extract (cryptotanshinone 3%): duckweed plant extract, Inc.;

aloperine (total alkali 65%): ningxia salt pond is produced by biochemical engineering Co., Ltd;

ormosidine (total alkali 60%): hubeixinkang pharmaceutical chemical Co., Ltd;

test strains: escherichia coli ATCC25922 and Staphylococcus aureus ATCC25923, which are purchased from Chinese veterinary drug inspection centers.

1.2 preparation of the Colophonium oil

Air drying aerial parts of fresh Bumper-top-bark incense, pulverizing, weighing 100g of coarse powder, extracting volatile oil by steam distillation for 4h, extracting distillate with petroleum ether to obtain light yellow liquid, drying with anhydrous sodium sulfate to obtain Bumper-top-bark incense volatile oil, and storing in a refrigerator for later use.

1.3 preparation of the bacterial suspension

Activating test strain with LB slant culture medium, inoculating LB liquid culture medium, shaking at 37 deg.C for 24 hr to obtain test strain solution, and preparing 10 with sterile normal saline⁶-10⁷cfu.ml^-1A bacterial suspension of individual bacteria.

1.4 determination of the bacteriostatic Effect

Diluting the Bumper-gum volatile oil with sterile water containing 5% Tween-80 to 100, 50, 10, 5, 2.5 and 1.25mg.ml respectively^-1Then respectively adding 1ml of diluted pelargonium odoratissimum volatile oil and 9ml of LB solid culture medium with 55 ℃ into each culture dish, and uniformly mixing to prepare 10, 5, 1, 0.5, 0.25 and 0.125mg.ml^-1Taking 10 mul of flat culture medium containing the pelargonium odoratissimum volatile oil after the flat culture medium is solidified⁶-10⁷cfu.ml^-1Bacterial suspensions of individual bacteria were spread on the surface of the plating medium, repeated 3 times per concentration. Taking LB solid culture medium without volatile oil of the inoculated bacterial suspension as a positive control, and taking LB solid culture medium with volatile matter in the sterile suspension as a negative control. And (5) culturing at the constant temperature of 37 ℃ for 24h, observing and counting, and evaluating the bacteriostatic effect.

The method is the same as the method for carrying out bacteriostasis experiments on 2 test bacteria by using the essential oil of the Bulbophyllum odoratissimum, the extract of the salvia miltiorrhiza, the aloperine, the ormosine and the complex thereof with the same bacteriostasis component concentration. The concentration of the bacteriostatic component in the culture medium and the bacteriostatic effect are shown in table 1.

TABLE 1 concentration of bacteriostatic component in culture medium and bacteriostatic effect

As can be seen from Table 1, the Bumper-top-gum volatile oil and aloperine have good bacteriostatic effects on Staphylococcus aureus and Escherichia coli. After the composition, the effect of the No. 6 antibacterial agent is greatly improved, which indicates that the composition has better coordination effect, and the mixture ratio of the No. 6 sample is determined as the optimal mixture ratio of the antibacterial components.

Second, stability and detergency test of disinfectant detergent for pets

2.1 preparation of disinfectant detergent for pets

The antimicrobial component was prepared according to the method of example 1, and the ratio was according to the ratio of the antimicrobial component No. 6. The disinfectant detergent for pet was prepared according to the method of example 2, and the weight ratio of each raw material is shown in table 2.

TABLE 2 raw materials ratio of disinfectant detergent for pets

2.2 appearance and stability testing of disinfectant detergents for pets

In Table 2, the products No. 1-5 are all clear, transparent and light yellow solutions, and are tasteless. Placing in an environment with the temperature of-5 ℃ for 24h, taking out, and placing at room temperature to slowly recover to the room temperature without crystallization and precipitation; placing in a constant temperature box at (40 +/-1) ℃ for 24h, taking out and immediately observing, wherein no layering and turbidity are generated, no odor is changed, and the stability is qualified.

2.3 detergency test of disinfectant detergent for pets

The test method comprises the following steps: weighing a group of 6 glass slides, weighing the mass M0 before smearing, then uniformly smearing oil stains on one side of the glass slide, drying for 4 hours, weighing M1, and controlling the smear amount of each group to be 0.65g +/-0.05 g. The mass M2 was measured after washing tests with the detergents sample No. 1-5, washing and drying.

And calculating the oil removal rate, repeating the experiment for 3 times for each sample, taking an average value, and controlling the relative average deviation to be less than or equal to 5%. The oil removal rate is calculated according to a formula.

Oil removal rate = (M1-M2)/(M1-M0)%

The results were averaged over three replicates for each sample according to the detergency test performed on samples 1-5, and the oil removal was calculated as the mean relative deviation, and the results are shown in table 3.

TABLE 3 detergency test data for each group

As shown in Table 3, the relative average deviation of each group of the detergency test data is less than 5%, which indicates that the detergency test data is reliable, wherein the detergency effect of No. 2 is the best, and the oil removal rate is 97.8% under the same conditions.

Sterilization effect observation of No. 2 and No. 3 pet disinfectant detergent

3.1 test materials

Test strains: representative strains of the sterilization test, staphylococcus aureus ATCC6538 strain, escherichia coli ATCC8099 strain, and candida albicans ATCC10231 strain, were used.

A disinfectant: the No. 2 disinfectant detergent prepared above.

Neutralizing agent: the compound neutralizer prepared from 3.0% of tween-80 and 0.3% of lecithin is adopted, and neutralization tests prove that the neutralizing effect is achieved before use.

3.2 test methods

3.2.1 neutralization assay

A chemical neutralization method is adopted, a neutralization solution is a compound neutralizing agent containing 3.0% of Tween-80 and 0.3% of lecithin, a test strain is staphylococcus aureus TCC6538, neutralization tests are divided into 8 groups according to the specification of disinfection and sterilization experimental specifications, ① disinfectant + test bacterial suspension → culture, ② (disinfectant + bacterial suspension) + neutralizing agent → culture, ③ neutralizing agent + bacterial suspension → culture, ④ (disinfectant + neutralizing agent) + bacterial suspension → culture, ⑤ PBS + bacterial suspension → culture, ⑥ pairs of tests are cultured in the same PBS → culture, ⑦ tests are cultured in the same neutralizing agent → culture, and ⑧ tests are cultured in the same culture medium → culture.

3.2.2 laboratory quantitative Sterilization test

A suspension quantitative sterilization test is adopted. Preparation of a microorganism content of 5 × 10⁵-5×10⁶cfu/ml bacterial suspension was used for the experiments. Placing 5.0ml of disinfectant in a test tube, placing in a water bath at 20 ℃ for 5 minutes, adding 0.1ml of bacterial suspension, uniformly mixing, respectively acting for 0.5, 1, 2, 5 and 10 minutes, sucking 0.5ml of the mixed solution, adding into a test tube of 1.5ml of neutralizer, uniformly mixing, respectively acting for 10 minutes, respectively taking 0.5ml, and counting viable bacteria by using a pouring method. The test samples were all incubated in an incubator at 37 ℃ for 48 hours, and the final results were observed.

3.2.3 organic matter Effect test

The bacterial suspension of the staphylococcus aureus added with 1% of the proteoliposa is prepared into the bacterial suspension of the staphylococcus aureus containing 50% and 25% of calf serum, a quantitative sterilization test of the suspension is carried out, and the method is compared with the method for comparing the bacterial suspension without the calf serum.

3.2.4 field test

30 New Zealand white rabbits are taken, 5 x 5cm squares are scribed at the adjacent parts of the dorsal ridge parts of the New Zealand white rabbits, and respectively taken as a test group and a control group, rabbit hairs of the control group are cut off during the test, the test group is placed in a test tube containing 10ml of neutralizer, 3ml of disinfectant is sprayed on the test group, the test group is combed by a wooden comb, the rabbit hairs are evenly distributed on the surface, the rabbit hairs are cut off after 10 minutes, the test tube containing 10ml of neutralizer is placed for neutralization for 10 minutes, the rabbit hairs are evenly shaken for analysis, and the results are observed after 48 hours.

3.2.5 stability test

The disinfectant was placed in a water bath at 54 ℃ for 14 days by an accelerated test method, and its killing effect on Candida albicans ATCC10231 strain was determined before and after the placement.

3.3 test results

3.3.1 neutralization test results

The results of the neutralization test are shown in Table 4.

Table 4 disinfectant neutralization test results

Staphylococcus aureus ATCC6538 strain was used as an indicator. As can be seen from Table 4, the neutralizing agent used was able to completely neutralize the bactericidal effect of disinfectant No. 2, and the neutralized product had no significant effect on the test microorganisms, indicating that the disinfection test could be carried out with this neutralizing agent.

3.3.2 laboratory quantitative Sterilization test results

The results of the sterilization tests are shown in Table 5.

TABLE 5 disinfectant Sterilization test results

As can be seen from Table 5, the disinfectant No. 2 can kill Candida albicans ATCC10231 strain by 100% in 2 minutes; the staphylococcus aureus ATCC6538 strain and the escherichia coli ATCC8099 strain can be killed by 1 minute and 2 minutes respectively to reach 99.99 percent.

3.3.3 organic influences test results

The staphylococcus aureus bacterial suspension containing 25 percent and 50 percent of calf serum and no calf serum acts for 5 minutes, and the killing rate reaches more than 99.90 percent. The disinfectant has no obvious influence on the killing effect of staphylococcus aureus protected by 25 percent and 50 percent of calf serum.

3.2.4 field test results

3ml of disinfectant is sprayed on the back test group area of the rabbit, and the rabbit is combed evenly by a wooden comb, so that natural bacteria carried in the rabbit hair can be killed by 90.50 percent in 10 minutes on average.

3.2.5 results of stability test

The killing effect of the disinfectant on candida albicans ATCC10231 strain before and after the standing is shown in table 6.

TABLE 6 disinfectant effect on Candida albicans before and after placing

As can be seen from Table 6, the killing rate of the disinfectant before and after the disinfectant is placed is not obviously reduced, and the time that the killing rate of the disinfectant before and after the disinfectant is placed on Candida albicans ATCC10231 strain reaches 99.9% is 5 minutes. The shelf life of the disinfectant may be one year.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The pet disinfectant detergent is characterized by being prepared from the following raw materials in parts by weight:

15 parts of fatty alcohol-polyoxyethylene ether sodium sulfate, 5-9 parts of alkyl glycoside, 1-5 parts of α -alkenyl sodium sulfonate, 0.8 part of sodium hydroxide, 15 parts of an antibacterial component and 59.2 parts of water;

the antibacterial component is prepared from the following raw materials in parts by weight:

2. The disinfectant detergent for pets according to claim 1, wherein the detergent is prepared from the following raw materials in parts by weight:

15 parts of fatty alcohol-polyoxyethylene ether sodium sulfate, 8 parts of alkyl glycoside, 2 parts of α -alkenyl sodium sulfonate, 0.8 part of sodium hydroxide, 15 parts of antibacterial component and 59.2 parts of water.

3. The disinfectant detergent as claimed in claim 1, wherein said antibacterial component is prepared from the following raw materials in parts by weight:

4. A process for producing a disinfectant detergent for pet animals according to any one of claims 1 to 3, comprising the steps of:

(3) and (3) adding the sodium fatty alcohol-polyoxyethylene ether sulfate into water with the temperature of 45 ℃, stirring at constant temperature until the sodium fatty alcohol-polyoxyethylene ether sulfate is dissolved, then adding the alkyl glycoside, α -sodium alkenyl sulfonate, sodium hydroxide and the antibacterial component obtained in the step (2), and stirring until the components are completely dissolved to obtain the disinfectant detergent for pets.

5. The method for preparing a disinfectant detergent for pets according to claim 4, wherein in the step (1), the water vapor extraction time is 4 hours, the extraction solvent is petroleum ether, and the drying treatment is performed by using anhydrous sodium sulfate as a drying agent.

6. The method for preparing a disinfectant detergent for pet animals according to claim 4, wherein in said step (2), the heating temperature is 35-37 ℃.

7. Use of the disinfectant detergent for pets according to any of claims 1 to 3 for pet cleaning.