CN106995804A - A kind of engineering bacteriophage quick detection microorganism of acetylcholinesterase mark - Google Patents
A kind of engineering bacteriophage quick detection microorganism of acetylcholinesterase mark Download PDFInfo
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- CN106995804A CN106995804A CN201710167108.3A CN201710167108A CN106995804A CN 106995804 A CN106995804 A CN 106995804A CN 201710167108 A CN201710167108 A CN 201710167108A CN 106995804 A CN106995804 A CN 106995804A
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Abstract
This work is intended have developed microorganism in the engineering bacteriophage kit Direct Analysis environmental and biological materialses that an acetylcholinesterase is marked, and the engineering bacteriophage of this acetylcholinesterase mark can be with specific recognition pathogenic microorganism.After bacteriophage identification combination microorganism is engineered, the DNA that its genetic engineering is transformed is injected in microbial body, the substantial amounts of bacteriophage with acetylcholine ester zymoprotein newly of recombination expression amplification in microbial body.Project main contents are：Key design is engineered bacteriophage and microorganism responsive materials recognition mechanism and kinetics, investigates these and recognizes the functional module responded action rule in microbial rapid detection and the instant expression analysis of microorganism.This work innovation is embodied in：Reference and reference are provided to solve to be related in microorganism detection method " online ", the electromechanical engineering of " portable " and " full-automatic " and biology Complex Problem.
Description
Technical field
A kind of engineering bacteriophage quick detection microorganism of acetylcholinesterase mark of present invention design.
Background technology
In marine environment, microbiological contamination, body eutrophication, microbiologic(al) corrosion, microorganism, which are stained, etc. is all
The apparent form of microbial threats human being's production life, is also the objective condition of rapid microbial detection technical need.Existing number
According to showing, the loss of microbiological contamination and microbial identification species speed are closely related, and the identification determination time is longer, loss
Also it is bigger.Because, one side microorganism growth and the speed propagated are fast, aggravate environmental pollution and human diseases；Separately
On the one hand microbe species can not be specified to lead to not implement specific aim protectiving scheme, and then cause some drugses or antiseptic
Abuse.In ISO4883-2003 standards, the microbial identification time is identified as the important of microorganism detection industrial grade division
Parameter.Therefore, taking effective method quick detection microorganism microorganism disease and is stained effective in reduction marine environment
Means.
Microorganism detection and the theory of identification are extensively dependent on the response of microorganism feature product（Such as ATP responses luciferase,
Acetylcholinesterase response chloro- 3- indoles-β-D galactosides of the bromo- 4- of 5- and H2S responses Fe2+ of E. coli secretion etc.）, it is micro-
Bloom biological cell surface antigentic specificity recognizes (such as antibody-microbial cell, agglutinin-antimicrobial surface glycogen, antibiotic-micro-
Biological surface specific site and aptamers-antimicrobial surface recognition site etc.) and microbial gene analysis（DNA and 16s
RNA）.For feature product response detection microorganism, it determines the microbe quantity in water sample, and its specificity is very weak.Pass through
Basionic is added in water sample, ATP in microbial cell is discharged, luciferase, this enzyme energy is added
Enough and ATP effects cause photochemical reaction, produce quantitative light intensity.To antimicrobial surface antigentic specificity identification for, itself in order to avoid
Microorganism is recognized and detects based on epidemic disease reaction, the data that this method is obtained are accurate, but can not be used as a kind of online live body
Detection instrument, is easily influenceed by extraneous factor.Our seminars have carried out a series of work in terms of microorganism detection：Explore
No signal mark based on enzyme linked immunoassay is quickly examined with the electro-chemistry immunity biology sensor of nano material signal mark
Survey marine microorganism.The expansion and comparative study of the two aspect work, new approaches are provided for the development of microorganism detection technology.It is right
For microbial gene analytical technology, it is widely used in microbial identification, and target spot primer used is generally according to micro- life
The 16S rRNA gene expression characteristicses sequences of thing and design, its test limit can reach 1 cfu mL-1, and weak point is to need spy
Different sample treatment and purifying, complex operation step have higher technical requirements.This project is from mentioned microorganism detection technique
Weak point is set out, and researches and develops portable microorganism care diagnostic instrument.
Acetylcholinesterase abbreviation AchE, the activity with carboxypeptidase and aminopeptidase.Acetylcholinesterase participates in cell
Development and maturation, can promote neuronal development and nerve regneration.Chubbe etc. research has shown that AchE has carboxypeptidase and ammonia peptide
The activity of enzyme.In vitro, AchE can hydrolyze enkephalins（Enk）And Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2（SP）, but growth hormone release inhibiting hormone can not be hydrolyzed（Som）And blood
Pipe pitressin（VSP）Deng.Further research has shown that, AchE the active site of its range of hydrolysed peptides and is used as esterase as peptase
Active site is different.It is worth noting that, many non-cholinergics of nervous system, the neuron containing a large amount of AchE also contains simultaneously
Various neuropeptide materials.SP energy cells such as Adorsal root ganglia are AchE strong positives.Nearest studies have shown that is highly purified
The AchE from electric eel electric organ or cow's serum there is the activity of protease sample or excision enzyme.For serum proteins, AchE
The scavenging action of C-terminal residue can be played.In addition, AchE protease sample effect also obtains the support of molecular biology evidence, ammonia
Base acid analysis shows that AchE protein molecules are similar to the amino acid sequence of protease sample restriction endonuclease and serum carboxypeptidase.
In the range of 36 residues of their C-terminal, there is 40% amino acid sequence identical with the active fragment of protease.
Microorganism detection technology and imperfections, in addition it is also necessary to sustainable development and innovation.Microorganism detection field innovative point may
Based in terms of following four：One is to utilize the application of micro-nano Novel electronic devices and photoelectric device in microorganism detection.Two are
Multifunction device integrated system is separated to microorganism, screened and tested and analyzed.Three be the analysis system combination of Multi-example
Multimode detecting system is analyzed microorganism or synchronized to multichannel data acquisition system.Four be based on smart mobile phone just
Formula real-time test (point-of-care testing, POCT) technology is taken, makes the portable device and mobile Internet of personalization
With reference to.These are all the directions of innovation and development microorganism detection research.This work is reflected using biomolecule functionalization micron openings
Fixed and analysis microorganism, its signal detected, which mostlys come from biomolecule and target spot microorganism detection and can extend microorganism, to be passed through
The time of micron openings, and then obtain related microbial standard curve.
The content of the invention
To achieve the above object, the present invention use technical scheme for：
A kind of engineering bacteriophage quick detection microorganism of acetylcholinesterase mark, it is characterised in that：Including specific
Bacteriophage, the acetylcholinesterasegene gene of functionalization.
Such as the bacteriophage of specificity microorganism in right 1, specificity microorganism includes bacteriophage, the gold for being directed to Escherichia coli
The bacteriophage of staphylococcus aureus, the bacteriophage of salmonella, the bacteriophage of vibrios, the bacteriophage of Enterobacter sakazakii, small intestine knot
The bacteriophage of enteritis Yersinia ruckeri, the bacteriophage of C.perfringens, the bacteriophage of clostridium botulinum, the bacteriophage of anaerobic bacteria,
The bacteriophage of bacillus cereus.
The enzyme functionalized bacteriophage such as acetylcholine ester in right 2：Its feature includes：T4 bacteriophages, T7 bacteriophages, P2 bite
Thalline, P22 bacteriophages, bacteriophage lambda, the bacteriophages of φ 29.
The enzyme functionalized bacteriophage such as acetylcholine ester in right 3, its feature is included in gp10B GFP terminal fusions
Acetylcholinesterasegene gene.
Brief description of the drawings
Fig. 1:The enzyme functionalized bacteriophage of acetylcholine ester
（1）It is engineered bacteriophage；（2）Head；（3）It is coated gp-10B albumen；（4）Interior nucleoprotein；（5）Gp16 albumen；（6）gp15
Albumen；（7）Gp14 albumen；（8）Connector gp8；（9）Gp10B- acetylcholine ester enzyme fusion proteins；（10）Gp6 and gp7 albumen；
（11）Gp11 and gp12 albumen；（12）Tail protein line gp17；（13）Afterbody；（14）For specified microorganisms host's associated proteins
（Escherichia coli, staphylococcus aureus, salmonella etc.）.
Fig. 2：Microbiological analysis system based on the enzyme functionalized bacteriophage of acetylcholine ester.
Fig. 3：Sulfate with different reducing bacteria, Escherichia coli, staphylococcus aureus, the detection number of salmonella detection
Value.
Embodiment
Below by embodiment, the present invention will be further described.
Embodiment 1：
The detection of sulfate reducing bacteria：
The microorganism used in experiment utilizes bacteriolyze meat soup（Peptone 1%, sodium chloride 1%, yeast extract 0.5%, the mL of water 100）Suspend
Culture, single bacterium colony is centrifuged ten minutes in 4500 revs/min after incubated overnight under the conditions of 30 DEG C, 200 turns of shaking tables, and is buffered with PBS
Solution is diluted to various concentrations.
By 100 μ L concentration（10 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（102 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（103 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（104 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（105 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（106 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（107 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（108 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
The data of summary, draw the standard curve of microorganism under various concentrations.
Embodiment 2：
E. coli detection
The microorganism used in experiment utilizes bacteriolyze meat soup（Peptone 1%, sodium chloride 1%, yeast extract 0.5%, the mL of water 100）Suspend
Culture, single bacterium colony is centrifuged ten minutes in 4500 revs/min after incubated overnight under the conditions of 30 DEG C, 200 turns of shaking tables, and is buffered with PBS
Solution is diluted to various concentrations.
By 100 μ L concentration（10 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposable training
Base is supported, and is reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nano Au colloid of acetylcholinesterase response
Body, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, it is dense that measurement obtains this
Microbial signals under degree.
By 100 μ L concentration（102 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（103 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（104 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（105 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（106 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（107 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（108 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
The data of summary, draw the standard curve of microorganism under various concentrations.
Embodiment 3：
Staphylococcus aureus detects
The microorganism used in experiment utilizes bacteriolyze meat soup（Peptone 1%, sodium chloride 1%, yeast extract 0.5%, the mL of water 100）Suspend
Culture, single bacterium colony is centrifuged ten minutes in 4500 revs/min after incubated overnight under the conditions of 30 DEG C, 200 turns of shaking tables, and is buffered with PBS
Solution is diluted to various concentrations.
By 100 μ L concentration（10 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（102 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（103 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（104 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（105 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（106 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（107 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（108 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
The data of summary, draw the standard curve of microorganism under various concentrations.
Embodiment 4：
Salmonella detection detection
The microorganism used in experiment utilizes bacteriolyze meat soup（Peptone 1%, sodium chloride 1%, yeast extract 0.5%, the mL of water 100）Suspend
Culture, single bacterium colony is centrifuged ten minutes in 4500 revs/min after incubated overnight under the conditions of 30 DEG C, 200 turns of shaking tables, and is buffered with PBS
Solution is diluted to various concentrations.
By 100 μ L concentration（10 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（102 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（103 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（104 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（105 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（106 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（107 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
By 100 μ L concentration（108 cfu ml-1）The bacteriophage of bacterium solution and acetylcholinesterase mark is added to disposably
Culture medium, and reacted 8 hours in 37 °C.Add the iodo acetylthiocholine and nanogold of acetylcholinesterase response
Colloid, is cultivated 30 minutes, with the excitation source actuated sensor platform of microbe diagnosis instrument, produces optical signal, measurement is somebody's turn to do
Microbial signals under concentration.
The data of summary, draw the standard curve of microorganism under various concentrations.
Claims (4)
1. a kind of engineering bacteriophage quick detection microorganism of acetylcholinesterase mark, it is characterised in that：Including specificity
Bacteriophage, the acetylcholinesterasegene gene of functionalization.
2. such as the bacteriophage of specificity microorganism in right 1, specificity microorganism includes bacteriophage for Escherichia coli, golden yellow
The staphylococcic bacteriophage of color, the bacteriophage of salmonella, the bacteriophage of vibrios, the bacteriophage of Enterobacter sakazakii, small intestine colon
The bacteriophage of scorching Yersinia ruckeri, the bacteriophage of C.perfringens, the bacteriophage of clostridium botulinum, the bacteriophage of anaerobic bacteria, wax
The bacteriophage of sample bacillus.
3. the enzyme functionalized bacteriophage such as acetylcholine ester in right 2：Its feature includes：T4 bacteriophages, T7 bacteriophages, P2 phagocytosis
Body, P22 bacteriophages, bacteriophage lambda, the bacteriophages of φ 29.
4. the enzyme functionalized bacteriophage such as acetylcholine ester in right 3, its feature is included in gp10B GFP terminal fusion second
Acetylcholinesterase gene.
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