CN105794702B - A kind of jellyfish acetabulum culture apparatus and its application method - Google Patents
A kind of jellyfish acetabulum culture apparatus and its application method Download PDFInfo
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- CN105794702B CN105794702B CN201610282101.1A CN201610282101A CN105794702B CN 105794702 B CN105794702 B CN 105794702B CN 201610282101 A CN201610282101 A CN 201610282101A CN 105794702 B CN105794702 B CN 105794702B
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- acetabulum
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Marine Sciences & Fisheries (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention discloses a kind of jellyfish acetabulum culture apparatus and its application method, its structure includes culture bottle and electrodynamic type roller bottle machine, the culture bottle includes the cylindrical shape body of both ends open, and the side cover that can be detachably connected with the body both ends, and there is hole in the centre of the side cover, hemispherical depression is evenly arranged on the body, during specific experiment, acetabulum can do movement of swimming with the effective flow of low speed inside culture bottle, cylindrical body is without dead angle and gas tube, effectively prevent a bulk damage, water body does not generate bubble simultaneously, it can be effectively prevented in acetabulum gastral cavity and enter bubble floating, reduce the death rate.Conscientiously the acetabular survival rate of jellyfish is improved, and ensure that the accuracy of desk research experiment.Be conducive to disastrous and economy jellyfish further investigation, all have been widely used in the Related Research Domains such as fishery resources utilization and restoration of the ecosystem.
Description
Technical field
The invention belongs to aquatic livestock breeding device technical fields.More particularly to the jellyfish disk like used in a kind of laboratory
Body culture apparatus and its application method.
Background technology
Since at the end of the 20th century, there are a large amount of of jellyfish and break out in many areas in the world, to ecological environment, fishery resources,
Sea-farming, littoral industry and tourist industry bring tremendous influence and loss.The whole world has more than 100 people to die of jellyfish to bite every year
Wound, 90% catching net damage due to jellyfish, and annual economic loss is up to 265,000,000 dollars.In the jellyfish that China coastal seas occurs
There are mainly four types of, including jellyfish, husky bruising, Cyanea nozakii and aurelia.Wherein, only jellyfish is fishery economic kind, remaining 3
Kind is disastrous jellyfish.In recent years, Qinhuangdao, Dalian, the more ground summer bathing beaches in Qingdao have tourist to be bitten or white by sand
The report that rosy clouds jellyfish sting is even died, Jiaozhou Bay aurelia break out invasion power plant and also result in local industrial resident's use
The once interruption of electricity.
Harm caused by a large amount of outbursts of disastrous jellyfish causes the highest attention of the whole society, for disastrous jellyfish
Research it is also more and more deep, history of life of several disaster jellyfishes has the alternation of generations, that is, swims the medusoma in stage and bottom is dwelt
The Xi shape body two eposides in stage.Xi shapes body generates acetabulum by strobilation, grows into young jellyfish.So acetabulum is to water
The Population Size of parent phase has great influence, and to jellyfish, acetabular research can preferably disclose the outburst of disaster jellyfish
Mechanism.However during the experiment, the acetabular indoor culture of related jellyfish is always difficult point.Adult jellyfish diameter is up to 2-3
Rice, tentacle filament length is up to 1 meter, but its newborn acetabulum diameter, less than 1 centimetre, Burden-Swimming Ability of KM is weak, can only be by surrounding water
Flow constriction umbrella diameter move about, ingest.At present, most of traditional training method adds glass tube air-charging incubation using beaker,
By causing seawater surge to seawater inflation in beaker, so as to drive acetabulum travelling, ingest.Disadvantage is that glass
Often there is flow dead angle there are inhomogeneities in beaker in flow caused by pipe inflation, once acetabulum enters without water
Stream, which drives, can not move about and ingest, and the individual in acetabulum culture is caused notable difference occur.And in incubation, with
Acetabular growth, the gas being filled with can often enter in umbrella portion gastral cavity, and acetabulum is caused to float on atrophy death in umbrella portion on the water surface,
Cause data human error.Therefore, there is an urgent need to develop a kind of novel acetabulum cultivation apparatus, to improve disk like
The accuracy of survival rate and the desk research experiment of body.
Invention content
The present invention provides the jellyfish acetabulum culture apparatus and its application method used in a kind of laboratory, existing to make up
There is the above-mentioned deficiency of technology.
Specifically, jellyfish acetabulum culture apparatus, including culture bottle and electrodynamic type roller bottle machine, the culture bottle includes both ends
The cylindrical shape body of opening and the side cover that can be detachably connected with the body both ends, and there is hole in the centre of the side cover, institute
It states and hemispherical depression is evenly arranged on body.And the culture bottle is transparent material, convenient for observation jellyfish growth.
Electrodynamic type roller bottle machine described above refers to be capable of providing the equipment that culture bottle rolls and sets rolling speed.
In techniques described above scheme, the setting of the hemispherical depression of cylindrical shape body, it is therefore an objective to make culture bottle body
Inland sea water flowing be more nearly nature seawater movement, while in culture bottle water movement at the uniform velocity, homogeneous, so as to drive acetabulum umbrella
Diameter contractile motion, be effectively reduced in incubation because of individual movement, ingest between caused by otherness and it is abiotic from
It is dead caused by body.
For jellyfish acetabulum culture apparatus described above, in preferred technical solution, the side cover is dish shape piece, tool
Body, the dish shape piece flare, both ends open, respectively coaxial wide opening and slot；Wide mouth end is provided with can be with body
The sealed connecting component being used cooperatively.More preferably in technical solution, the dish shape piece slot end port protrude in bottleneck shape and
Helicitic texture is provided with, wide mouth end port is provided with the helicitic texture that can be used cooperatively with body both ends.
For jellyfish acetabulum culture apparatus described above, in preferred technical solution, ring type seal is further included；Institute
The both ends for stating ring type seal are respectively provided with the sealed connecting component that can be used cooperatively with dish shape piece slot end.By setting multiple annulars
Multiple culture bottles can be connected, while cultivate multiple research objects by sealing ring, and drive series connection by external mechanical devices
Culture bottle common rotation, while rotate and identical environment is provided to experimental subjects, reach indifference between the individual of acetabulum culture,
Data is more accurate.More preferably in technical solution, the ring type seal is ring screw circle, and both ends have can be with
The helicitic texture that dish shape piece slot end bottleneck is used cooperatively.
For jellyfish acetabulum culture apparatus described above, in preferred technical solution, sieve, the sieve are further included
For 100-150 mesh, it is detachably arranged between ring type seal and dish shape piece slot end.Under normal conditions, laboratory is normal
Bolting silk, nylon wire or tiffany may be selected in sieve.The purpose for placing sieve is artificially by acetabulum when preventing from taking culture bottle
It pours out or is mixed into other culture bottles.
For jellyfish acetabulum culture apparatus described above, in preferred technical solution, the sealed connecting component is spiral shell
Line connector, patch all structures that can realize sealed connection such as sealing element or clamp connection sealing element can.
For jellyfish acetabulum culture apparatus described above, in preferred technical solution, flat sealing cover, institute are further included
State flat sealing cover removably can closely connect with the slot end of dish shape piece.The use of flat sealing cover, in typically testing,
It is used during jellyfish growth conditions for observing.
For jellyfish acetabulum culture apparatus described above, in preferred technical solution, body sealing cover, institute are further included
State body sealing cover removably can closely connect with the both ends of body.
Another aspect of the present invention also resides in the application method for protecting device described above, including following operating procedure：
1. culture apparatus assembles：It is one group by n body, 2n dish shape piece, n-1 ring type seal series connection, is put
In on electrodynamic type roller bottle machine；
2. adding in filtering sea into body respectively, the peak level of seawater is less than dish shape piece slot end in the body,
Appropriate electrodynamic type roller bottle machine speed is adjusted, in constant temperature illumination box, cultivates the aurelia ephyra just released.
For the application method of device described above, in preferred scheme, step is 1. in the assembling process of middle culture apparatus,
Sieve can also be installed in place of dish shape piece and ring type seal series connection.Prevent from taking culture bottle when artificially acetabulum poured out or
Person is mixed into other culture bottles.
Jellyfish acetabulum cultivation apparatus of the present invention, stable structure are readily produced, and material is easy to get, production cost
It is low, it assembles and simple using operating method, conducive to acetabulum culture；Its specific effect is verified in testing indoors.
During specific experiment, acetabulum can do movement of swimming with the effective flow of low speed inside culture bottle, and cylindrical body is without dead angle
And gas tube, a bulk damage is effectively prevented, while water body does not generate bubble, can be effectively prevented in acetabulum gastral cavity and enter
Bubble floating reduces the death rate.Conscientiously the acetabular survival rate of jellyfish is improved, and ensure that the accurate of desk research experiment
Property.Be conducive to disastrous and economy jellyfish further investigation, utilize in fishery resources and led with correlative studys such as restorations of the ecosystem
Domain all has been widely used.
Description of the drawings
Each block diagram of Fig. 1 devices of the present invention；
The multiple culture bottles of Fig. 2 are used in series schematic diagram；Culture bottle
Fig. 3 jellyfish acetabulum Laboratory culture device use state diagrams；
Wherein：1. body, 2. dish shape pieces, 3. body sealing covers, 4. flat sealing covers, 5. hemispherical depressions, 6. annular spiral shells
Line circle, 7. electrodynamic type roller bottle machines.
Specific embodiment
Following non-limiting examples can make those of ordinary skill in the art be more fully understood the present invention, but not with
Any mode limits the present invention.
1 acetabulum culture bottle of embodiment is used alone
Below in conjunction with attached drawing 1, the structure of jellyfish acetabulum culture bottle of the present invention is described, including body 1,
Dish shape piece 2, body sealing cover 3, flat sealing cover 4 and electrodynamic type roller bottle machine 7；The body 1 is cylindrical, and surrounding is uniformly set
Hemispherical depression 5 is equipped with, the both ends port of cylindrical shape body 1 is both provided with external screw thread；2 flare of dish shape piece, wide opening
End port is provided with the internal thread that can be used in combination with 1 both ends external screw thread of body, and slot end port is protruded in bottleneck shape, and is set
It is equipped with external screw thread；The body sealing cover 3 is matched with internal thread, and with the external screw thread of the both ends port of body 1；The annular
The fillet of screw 6 is matched with internal thread, and with the external screw thread of the both ends port of body 1；The flat sealing cover 4 with internal thread, and
It is matched with the external screw thread of the slot end bottleneck of dish shape piece 2.
In addition, further include ring screw circle 6；The both ends of the ring screw circle 6 are respectively provided with can be with 2 slot end of dish shape piece bottle
The internal thread that mouth is used cooperatively.It is additionally included in the dismountable mesh number set between 2 slot end bottleneck of ring screw circle 6 and dish shape piece
Bolting silk for 100-150 ranges.
The structure of the cylindrical shape body 1, dish shape piece 2 and body sealing cover 3, is connected by helicitic texture, is used cooperatively
In the case of, it disclosure satisfy that common jellyfish acetabulum culture.
The series connection of more than 2 a culture bottle of embodiment is one group of use
On the basis of 1 technical solution of embodiment, for the ease of to acetabular research, usually experiment can set control group,
The sample sets and parallel group of the different factors, identical culture environment are more advantageous to acetabular research, therefore present invention design
Ring screw circle 6 can be used as connector that will be connected by multiple culture bottles be one group, such as Fig. 2, i.e., ring screw circle 6 is interior
Screw thread both ends can be connected with 2 slot end of dish shape piece simultaneously；So as to achieve the purpose that be used in series multiple culture bottles.
Take in order to prevent series connection culture bottle when, artificially the acetabulum in different culture bottles is poured out or is mixed into other
In culture bottle, the bolting silk structure placed between 2 slot end bottleneck of ring screw circle 6 and dish shape piece has also been devised in the present invention.
The mode being connected through a screw thread, it is one group that n body 1,2n dish shape piece 2, n-1 ring screw circle 6, which are connected,
Such as Fig. 2, place it on electrodynamic type roller bottle machine 7, specific application method is as follows：
By taking n=3 as an example, two dish shape pieces 2 are first screwed in 1 both ends of body by when daily culture, will with two ring screw circles 6
Three culture bottle series connection, two dish shape pieces, the 2 slot end bottleneck of most end place bolting silk and screw on ring screw circle 6；Three cultures
Bottle connects into a culture bottle, and culture needs to ventilate, therefore culture bottle both ends are not sealings, and the peak level for cultivating water body is low
In 2 slot end of dish shape piece.Culture bottle is positioned on electrodynamic type electrodynamic type roller bottle machine 7, such as Fig. 3, utilizes 7 band of electrodynamic type roller bottle machine
Seawater in dynamic culture bottle flows, and the hemispherical depression 5 on body 1 makes acetabulum rolling be conducive to its movement and ingest.
In experimentation, when needing to observe and measure, multiple culture bottles can be divided by way of removing ring screw circle 6
It opens, is twisted with flat sealing cover 4 on dish shape piece 2, the dish shape piece 2 of the culture bottle other end is removed that change body into close by overturning culture bottle
Capping 3, overturns again, removes to be positioned under stereomicroscope after opposite side dish shape piece 2 and observes and measures.
As embodiment, culture bottle main body of the invention uses transparent material, 1 high 70mm of body, and 1 overall diameter of body is
100mm, 2 wide mouth end screw socket interior diameter 100mm of dish shape piece, 2 slot end screw socket overall diameter 20mm of dish shape piece, in body sealing cover 3
Diameter 100mm, ring screw circle 6 and 4 interior diameter of flat sealing cover are 20mm.
3 jellyfish acetabulum Laboratory culture method of embodiment
Using the laboratory cultures method of the jellyfish acetabulum culture apparatus described in embodiment 2, it is as follows：By reality
The mode for applying example 2 forms culture bottle, the filtering sea 200ml of 20 DEG C of temperature, salinity 30 will be added in culture bottle, takes and just release
Aurelia ephyra, umbrella diameter 4mm or so is put into 10 ephyras, with two ring screw circles in each culture bottle
6, bolting silk is placed at both ends respectively, then above three culture bottle is connected, and as an experimental group, culture bottle is put in electrodynamic type rolling
On bottle machine 7, speed is set as 2 revs/min, starts to cultivate in constant temperature illumination box, feeds artemia 3 times a day without section children
Body.First electrodynamic type roller bottle machine 7 is suspended when feeding, ring screw circle 6 is backed out and removes bolting silk and added in artemia with suction pipe, places sieve
Thin,tough silk screws on ring screw circle 6, starts electrodynamic type roller bottle machine 7.Water is changed after ingesting 1 hour every time.Next culture bottle is taken when changing water
Unit topples over seawater from the side dish shape piece 2 for having bolting silk, and opposite side dish shape piece 2 screws on flat sealing cover 4, and culture bottle is turned over
Turn, 4 one end of flat sealing cover is downward, and stick at bolting silk if any acetabulum takes fresh filtering sea to rinse with suction pipe, adds in quantitative new
Fresh filtering sea holds up culture bottle dish shape piece 2 and is located at bottle both sides, removes flat sealing cover 4 and puts bolting silk and screws on ring screw
Circle 6.Microscopic observation measures acetabulum umbrella diameter and individual state every three days for culture.
Observation selection backs out ring screw circle 6 and removes the side of bolting silk dish shape piece 2 and screw on flat sealing cover 4 after water is changed,
4 one end of culture bottle overturning flat sealing cover are downward, and the dish shape piece 2 for backing out upper end screws on body sealing cover 3, overturns culture bottle bottle
3 one end of body sealing cover is downward, backs out the dish shape piece 2 of upper end, by this culture bottle as being observed and measured under Stereo microscope, records umbrella
Diameter observes individual state and feeding state.Culture bottle schematic diagram when Fig. 3 is observation；Extra large moon acetabulum in experiment can be at 10 days
After grow into 20mm or so, no dead individuals, individual growing homogenous.It is identical simultaneously using traditional experiment room beaker air-charging incubation
Envirment factor and the bait frequency, data comparison are as follows.Conventional pneumatic culture is found in from experimental result to compare, and is set using the present invention
The culture apparatus of meter rolls the acetabulum of culture, and average daily growth rate is high, and individual survival rate is high, and individual growth difference is small.
Claims (9)
1. a kind of jellyfish acetabulum culture apparatus, which is characterized in that including culture bottle and electrodynamic type roller bottle machine, the culture bottle packet
The side cover that includes the cylindrical shape body of both ends open and can be detachably connected with the body both ends, and the centre of the side cover
There is hole, hemispherical depression is evenly arranged on the body.
2. jellyfish acetabulum culture apparatus according to claim 1, which is characterized in that the side cover is dish shape piece, described
Dish shape piece flare, both ends open, respectively coaxial wide opening and slot；Wide mouth end is provided with what can be used cooperatively with body
Sealed connecting component.
3. jellyfish acetabulum culture apparatus according to claim 2, which is characterized in that further include ring type seal；It is described
The both ends of ring type seal are the sealed connecting component that can be used cooperatively with dish shape piece slot end.
4. jellyfish acetabulum culture apparatus according to claim 3, which is characterized in that further include sieve, the sieve is
100-150 mesh is detachably arranged between ring type seal and dish shape piece slot end.
5. jellyfish acetabulum culture apparatus according to claim 4, which is characterized in that the sealed connecting component connects for screw thread
Fitting patches sealing element or clamp connection sealing element.
6. jellyfish acetabulum culture apparatus according to claim 3, which is characterized in that flat sealing cover is further included, it is described
Flat sealing cover removably can closely be connect with the slot end of dish shape piece.
7. jellyfish acetabulum culture apparatus according to claim 1, which is characterized in that body sealing cover is further included, it is described
Body sealing cover removably can closely be connect with the both ends of body.
8. a kind of application method of claim 3 described device, which is characterized in that it includes following operating procedure：
1. culture apparatus assembles：It is one group by n body, 2n dish shape piece, n-1 ring type seal series connection, places it in electricity
On dynamic formula roller bottle machine；
2. adding in filtering sea into body respectively, for the peak level of the seawater less than dish shape piece slot end, it is appropriate to adjust
Electrodynamic type roller bottle machine speed in constant temperature illumination box, cultivates the aurelia ephyra just released.
9. application method according to claim 8, which is characterized in that step is 1. in the assembling process of middle culture apparatus,
Dish shape piece and ring type seal series connection part installation sieve.
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| CN201610282101.1A CN105794702B (en) | 2016-04-29 | 2016-04-29 | A kind of jellyfish acetabulum culture apparatus and its application method |
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| CN201610282101.1A CN105794702B (en) | 2016-04-29 | 2016-04-29 | A kind of jellyfish acetabulum culture apparatus and its application method |
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| CN105794702B true CN105794702B (en) | 2018-07-10 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107705868B (en) * | 2017-09-14 | 2024-01-05 | 华北电力大学 | Radionuclide collection device in ocean |
| CN107568113B (en) * | 2017-09-20 | 2019-08-09 | 辽宁省海洋水产科学研究院 | A kind of control technique of more batches of strobilations of jellyfish Xi shape body |
| CN107455317B (en) * | 2017-09-22 | 2022-10-14 | 辽宁省海洋水产科学研究院 | Automatic water changing system for jellyfish breeding and application thereof |
| CN115104555B (en) * | 2022-08-29 | 2023-01-24 | 太平洋海底世界博览馆有限公司 | Pacific ocean acantholepsis breeding method |
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| CN202222282U (en) * | 2011-05-23 | 2012-05-23 | 上海晶海实业有限公司 | Small jellyfish tank for ornamental jellyfish culture |
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| JP2009044979A (en) * | 2007-08-17 | 2009-03-05 | Shizuoka Prefecture | Device for rearing aquatic organism |
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| CN202730112U (en) * | 2012-08-21 | 2013-02-13 | 孙立群 | Culture tank |
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