CN105685061A - Application of benziothiazolinone in preventing and treating potato seed-borne diseases and promoting rooting and germination - Google Patents
Application of benziothiazolinone in preventing and treating potato seed-borne diseases and promoting rooting and germination Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
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Abstract
The invention discloses application of benziothiazolinone, 3% benziothiazolinone wettable powder and water dispersible granules in potato crops. Potato seed-borne diseases can be prevented and treated, and rooting and germination can be promoted.
Description
Technical field
The present invention relates to benziothiazolinone and process seed preventing and treating (seed soaking) disease and the use application promoting seed to take root
Technical background
Benziothiazolinone (Benziothiazolinone) belongs to different thiazoles compound, it is a kind of efficient, low toxicity, broad-spectrum germicide, its mechanism of action is: with antibacterial, the anion binding of the surface of cell membrane of mycete or react with sulfydryl, destroy the synthesis system of protein and cell membrane, thus suppressing antibacterial, mycete breeding, interference pathogen cell metabolism so that it is physiologic derangement, causes that pathogen is dead。Antibacterial, fungus-caused corps diseases are had good prevention effect by this compound, and crops are fool proof。
Rhizoma Solani tuber osi staple food grain, Rhizoma Solani tuber osi seed-borne disease is the Major Diseases that harm Rhizoma Solani tuber osi produces, and such as potato scab, balck shank affect the yield and quality of China Rhizoma Solani tuber osi for a long time。Current China preventing and treating Rhizoma Solani tuber osi disease mainly or uses the single medicament such as antibiotics, traditional copper preparation, and effect is very undesirable, makes the continuous and effective preventing and treating of Rhizoma Solani tuber osi disease face a severe challenge。
Summary of the invention
It is an object of the invention to provide the application on preventing and treating Rhizoma Solani tuber osi seed-borne disease and short root are germinateed of a kind of benziothiazolinone。Described disease is fungus, bacterial disease。Benziothiazolinone can prevent and treat these diseases by seed treatment, such as potato scab, balck shank, and promotes that root germinates and improves yield, compared with traditional medicament, improves prevention effect, delays seed-borne disease to occur, reduces dosage。
It can adopt wettable powder, water dispersible granules or other preparation, and wherein the mass percent of benziothiazolinone can be 3% or other percentage ratio。
Benziothiazolinone is possible not only to adopt the form of single dose, it is possible to adopting composite form, the active component composite with benziothiazolinone can be other antibacterial, and such as Fluoxastrobin, thifluzamide etc. have synergistic function。Active component is allocated the insecticides adjuvant of corresponding dosage form into and be can be made into wettable powder, water dispersible granules, kind clothing suspending agent etc.。
The present invention prominent effect on preventing and treating Rhizoma Solani tuber osi seed-borne disease and short root are germinateed。And, the present invention can promote potato plant to emerge in advance by benziothiazolinone, promote growth, shorten contacting of pathogenic bacteria and plant tender tissue, decrease the probability of infection process, and the disease resistance of potato plant is improve when infection process, decrease the generation of disease, thus further increasing prevention effect, it is possible to effectively prevent and treat the such as Rhizoma Solani tuber osi such as potato scab, balck shank seed-borne disease。
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described, and in embodiment, the percentage ratio of each component is all weight percentage, but the present invention is not limited to the proportion in following exemplary case。
Test of pesticide effectiveness example 1
With the indoor Security test to potato sprouting of mass percent 3% benziothiazolinone wettable powder
Test carries out with reference to " pesticide is to crop safety interpretational criteria (NY/T1965.1-2010) "。
Biological statistical method: test data is carried out statistical analysis by the new multipole of Deng Kenshi poor (DMRT) method。
EXPERIMENTAL DESIGN and application method: test sets No. 3 whole potatos of Gansu Province potato, potato No. 3 strippings and slicings in Gansu Province (cut kind with sterilization cutter, every piece of whole potato is cut to two pieces) and the whole potato of micro potato respectively with 3% benziothiazolinone wettable powder 200 times, 500 multiple doses process, amount to 6 respectively to process with clear water process for blank, in triplicate。
Test and require potato block is carried out spraying process according to chemicals treatment February 14 in 2015, after potato block surface is dried, carry out indoor germination test。Indoor germination test often processes 90 potato seeds, often repeats 30。Greenhouse test sowing time of emerging is on February 15th, 2015, each processes 20 plantation 1 row。
Control time and number of times: investigation on February 24th, 2015 indoor germination test each process Rhizoma Solani tuber osi shows money or valuables one carries unintentionally rate, investigation on March 2 germination percentage, investigation on March 17 emergence rate, April 9 investigation greenhouse root growing state, investigation on April 16 plant height。
Table 1, the 3% benziothiazolinone wettable powder safety testing result to potato sprouting
Table 2, the safety testing result that Rhizoma Solani tuber osi is emerged by 3% benziothiazolinone wettable powder
Interpretation of result: 3% benziothiazolinone wettable powder is to the safety results of potato sprouting such as shown in table 1, table 2。
Greenhouse result of the test of emerging is as shown in table 3
Indoor germination test investigation on February 24th, 2015 shows, each potato block that processes all shows money or valuables one carries unintentionally；Investigation on March 2nd, 2015 shows that reason potato block all germinates everywhere。It is normal that each dosage of test medicine 3% benziothiazolinone wettable powder processes (200 times, 500 times) potato sprouting, chemicals treatment blank germination percentage difference is not notable, and the bad growth conditions such as variable color, necrosis, wilting all do not occur, illustrate reagent agent 3% benziothiazolinone wettable powder on potato sprouting without impact。
The table 33% benziothiazolinone wettable powder safety testing result to Rhizoma Solani tuber osi
Greenhouse result of the test of emerging shows, the each dosage of test medicine 3% benziothiazolinone wettable powder processes (200 times, 500 times) Rhizoma Solani tuber osi and emerges and grow normal, chemicals treatment blank difference is not notable, and all not bad growth conditions, illustrate that Rhizoma Solani tuber osi is emerged by reagent agent 3% benziothiazolinone wettable powder and the growth of root and plant is without impact。
Finding in investigation, indoor germination test stripping and slicing processes phenomenon of losing water, and there is the metachromatism caused because humidity is relatively low on all process bud tops。Test survey of emerging shows that the growth conditions of each process growth germination, bud and plant is good, zero difference compared with the control, and bud, young root and plant relative growth gesture that stripping and slicing processes are better。
Safety evaluatio: result of the test illustrate, 3% benziothiazolinone wettable powder to potato sprouting and emerge safety, without poisoning。
Test of pesticide effectiveness example 2
Experiment purpose:
1) .3% benziothiazolinone wettable powder 10 times, 15 times, 20 times liquid process micro potato, explore whether press down bud。
2) .3% benziothiazolinone wettable powder 10 times, whether shot hole is played inhibitory action after 15 times, 20 times immersion kinds。
Experimentation and result:
1). end on May 31st, 2015 and complete the plantation work of three batches of micro potatos, under fruit frame, seedlings nursing plate, accelerating germination environment, complete experiment respectively。
2) .3% benziothiazolinone wettable powder 10 times, 15 times, 20 times process complete first seed in spite of illness, soil in spite of illness experiment, complete the growth in later stage at fruit small basket；3% benziothiazolinone wettable powder 10 times process completes the experiment of the anosis micro potato of second batch, completes growth at seedlings nursing plate；Clear water has processed the experiment of the 3rd batch of anosis micro potato, and accelerating germination completed growth at seedlings nursing plate after 15 days。
3). find from experimental result, after 3% benziothiazolinone wettable powder 10-20 times is soaked seed, it does not have substantially press down bud situation, 3% benziothiazolinone wettable powder 20 times process group, best at fruit small basket growing state。
Test of pesticide effectiveness example 3
Experiment purpose:
1). explore 3% benziothiazolinone wettable powder and soak seed on the impact sprouted；
2). explore 3% benziothiazolinone wettable powder to whether Rhizoma Solani tuber osi root growth has facilitation。
Experimentation and result:
1). choose healthy Xia Bodi micro potato anosis, of uniform size 30, be divided into 5 groups；
2). take 5 dixie cups numbering 1,2,3,4,5 respectively, by ready napkin moistening, be placed in dixie cup as bottom；
3). the micro potato being divided into group is respectively put in dixie cup；
4). by the Tissue Cover of moistening on micro potato, and with sack, 5 groups are sealed。
Test result indicate that: 3% benziothiazolinone wettable powder 10 times immersion kind 30, having 25 germinations, it does not have press down bud phenomenon, 25 seedling sprout growing ways are highly uniform, emerge neat, root system is good。All the other 5 cut-offs were not unearthed June 30, agnogenio。
Experiments show that: the root system of Rhizoma Solani tuber osi be after tuber rudiment as the long 3cm-4cm of bud, occur out from the base portion of bud, constitute and mainly absorb root system, claim primary root or eye root。Later along with the growth of bud, on the leaf segment of bud with there is stolon while, there is 3-5 bar root, long about 20cm, round stolon, claim to crawl root。Primary root elder generation horizontal growth about arrives 30cm, and then vertically downward as deep as 60cm-70cm, root of crawling is mainly horizontal growth。
After being processed by 3% benziothiazolinone wettable powder presoaking and germinating, Rhizoma Solani tuber osi, when bud is not to 3 4cm, begins to the base portion from bud and starts to grow primary root, allows the growth of Rhizoma Solani tuber osi maintain by root system in advance。So female potato is had the effect of the short root growth of certain accelerating germination by 3% benziothiazolinone wettable powder。
Test of pesticide effectiveness example 4: by indoor virulence test, this example shows that potato scab bacterium is had good inhibiting effect by 3% benziothiazolinone wettable powder。
Test method
Adopt growth rate method, cultivate the bacteriostatic activity measuring reagent agent with toxic medium。On the basis of pilot study, if 5 concentration: effective ingredient 5,6,7.5,10,15 μ g/mL (preparation diluent 6000 times, 5000 times, 4000 times, 3000 times, 2000 times), aseptically, medicament is mixed with oat-agar cultures base, make the toxic culture medium of reagent agent, with the oat-agar cultures base flat board of not adding medicine for blank, shake up, pour into while hot and 90mm culture dish is made flat board, access, after flat board solidification, the bacterium cake (Φ=5.0mm) that growth is consistent。Often process and repeat 3 times, cultivate at 32 DEG C。
Cover with after culture dish until blank, measure each process colony diameter by decussation method, obtain each concentration suppression ratio to pathogenic bacteria by following formula formula。Converting the colony growth suppression ratio of each concentration to probit value, concentration converts logarithm value to。Then linear regression equation, EC are obtained with log concentration-inhibition percentage probit value50Value。
Result and analysis
The Toxicity Determination result of potato scab bacterium is shown in following table by 3% benziothiazolinone wettable powder。Can be seen that from the table below, potato scab bacterium is had stronger inhibitory activity by 3% benziothiazolinone wettable powder, on the oat-agar cultures base of pastille, the potato scab bacteria growing that each dosage processes all is suppressed, and suppression ratio raises with the increase of drug concentration, drug concentration and inhibitory action are proportionate, and correlation coefficient is 0.9928, there is strong correlation, the 3% benziothiazolinone wettable powder EC to potato scab bacterium50It is 1.2032 μ g/mL。
The table 43% benziothiazolinone wettable powder Toxicity Determination result to potato scab bacterium
Conclusion:
Can be seen that from measurement result, potato scab bacterium is had higher biological activity by 3% benziothiazolinone wettable powder, and EC50 is 1.2032 μ g/mL。
Test of pesticide effectiveness example 5: carry out the preventing and treating of potato scab in base, horse lane, Guyuan County。
Test method: select the potato seed that healthy Rhizoma Solani tuber osi will be greater than 40g to be prior to seeding cut into the potato block of 30-50g, each potato block is with eye, then the potato seed solution of 3% benziothiazolinone wettable powder is soaked seed, medicament during seed soaking: water: the ratio of seed is 1:500:10000, and carry out ditch spread in sowing time with 80g/ mu of carefully stopping, the soil selecting non-solanaceous crops is cultivated with carrying out ridge, row spacing 90cm, depth of planting 20cm, spacing in the rows is at 15cm, thickness of sowing is 4800 plants/acre, long-term every mu of potato raw applies carbamide 30kg, calcium superphosphate 100kg, potassium sulfate 80kg。Formulation rate at knot first 7 days drip irrigation 3% benziothiazolinone wettable powders of potato phase is 150g/ mu, and the formulation rate of drip irrigation 3% benziothiazolinone wettable powder is 100g/ mu when August mercury dropped, the preventing and treating of potato scab is carried out, with conventional dose test for comparison by above medicament and method。
In the potato harvest phase, testing site randomization being investigated, with routine administration (agricultural streptomycin) for comparison, each test clicks 5 investigation, 40 potato tuberss of each investigation, and calculates sickness rate, disease index and preventive effect。
Potato scab potato block-grading standard:
0 grade, potato skin is healthy, without scab；
1 grade, potato this health of scytoblastema, there is 1-2 fragmentary scab, shared area is not less than potato skin 1/4；
2 grades, potato skin has 3-5 scab, and shared area is not less than potato skin 1/4-1/3；
3 grades, potato skin has 5-10 scab, and shared area is not less than potato skin 1/3-1/2；
4 grades, seriously susceptible, scab is more than 10 or exceed that potato surface is long-pending 1/2。
Sick potato rate (%)=(in spite of illness tuber number/investigate total tuber number) × 100
Potato block disease index=[∑ (sick potato number × relative level numerical value at different levels)/(investigating total potato block number × 4)] × 100 prevention effects (%)=[(comparison potato block disease index-process potato block disease index)/comparison potato block disease index] × 100
Result of the test: use the prevention and controls of 3% benziothiazolinone wettable powder can effectively reduce sickness rate and the disease index of potato scab as can be seen from Table 5, preventive effect is 50.62%, wherein the sick potato rate of potato scab experimental field is 18.50%, and comparatively potato scab disease potato rate is 33.30%, experimental field potato scab disease index is 5.62,, comparatively potato scab disease index is 11.38。
Table 5 potato scab prevent and treat result
Conclusion: by using 3% benziothiazolinone wettable powder and this prevention and controls can effectively prevent and treat the generation of potato scab。
Claims (4)
1. benziothiazolinone application on preventing and treating Rhizoma Solani tuber osi seed-borne disease and short root are germinateed。
2. benziothiazolinone wettable powder, water dispersible granules preventing and treating Rhizoma Solani tuber osi seed-borne disease and short root germinate on application。
3. the benziothiazolinone wettable powder of mass percent 3%, water dispersible granules preventing and treating Rhizoma Solani tuber osi seed-borne disease and short root germinate on application。
4. the application on Rhizoma Solani tuber osi seed-borne disease and short root are germinateed of the benziothiazolinone as described in claim 1,2 or 3, it is characterised in that described disease is fungus, bacterial disease。
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| CN106561676A (en) * | 2016-11-10 | 2017-04-19 | 江苏辉丰农化股份有限公司 | Bactericide composition containing benzisothiazolones and coumoxystrobin |
| CN106359405A (en) * | 2016-11-11 | 2017-02-01 | 江苏辉丰农化股份有限公司 | Bactericide composition containing benzisothiazolone and ethylicin |
| CN106577721A (en) * | 2016-11-11 | 2017-04-26 | 江苏辉丰农化股份有限公司 | Bactericidal composition containing benzisothiazolinone and moroxydine hydrochloride |
| CN106818783A (en) * | 2017-01-06 | 2017-06-13 | 江苏辉丰农化股份有限公司 | A kind of microbicide compositions comprising BIT class and tetraconazole |
| CN106942240A (en) * | 2017-02-28 | 2017-07-14 | 江苏辉丰农化股份有限公司 | A kind of microbicide compositions comprising emodin derivates and BIT |
| CN109897012A (en) * | 2019-03-08 | 2019-06-18 | 西安临港科技创新发展有限公司 | A kind of benziothiazolinone complex and its preparation method and application with bactericidal activity |
| CN114946867A (en) * | 2020-12-31 | 2022-08-30 | 江苏省农业科学院 | Method for promoting rice root growth by using benziothiazolinone |
| CN114946867B (en) * | 2020-12-31 | 2023-06-16 | 江苏省农业科学院 | Method for promoting rice root growth by using benziothiazolinone |
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