CN105494369A - Composition containing gliotoxin - Google Patents

Composition containing gliotoxin Download PDF

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Publication number
CN105494369A
CN105494369A CN201510869430.1A CN201510869430A CN105494369A CN 105494369 A CN105494369 A CN 105494369A CN 201510869430 A CN201510869430 A CN 201510869430A CN 105494369 A CN105494369 A CN 105494369A
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grams
gliocladin
composition
acid
derivatives
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王成
宋妍
许兴志
邓晓杰
逄廷超
刘彦
吴磊
朱莹
赵彦龙
苑敬林
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Jingbo Agrochemicals Technology Co Ltd
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Jingbo Agrochemicals Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Pest Control & Pesticides (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides composition containing gliotoxin. The composition comprises gliotoxin or a derivative thereof and compounds A, wherein the compounds A comprise multiple compounds. The composition has good control effect on multiple plant viruses, bacteria, powdery mildew and downy mildew and has a synergistic effect when compounded with selected components, the dosage is reduced, and the composition meets the requirements of environmental protection and future national policies.

Description

A kind of composition containing gliocladin
Technical field:
The invention belongs to agriculture application, be specifically related to a kind of composition containing gliocladin.
Background technology:
Gliocladin is a kind of efficient germicide, also known as gliotoxin, and molecular formula: C 13h 14n 2o 4s 2, relative molecular weight: 326.4.Gliocladin is the diketopiperazine class secondary metabolite extracted from Trichoderma fermentation mycelium, for the Small molecular of single molecular structure, there is immunoregulatory activity, antimicrobial spectrum is wider, energy mould fungus inhibition, bacterium and virus, application report in medicine, to person poultry safety, low toxicity, easily degrades at occurring in nature.Be suitable for the production of pollution-free green food.
This product has application report in medicine, does not also report in agricultural chemicals.Because gliocladin antimicrobial spectrum is wider, energy mould fungus inhibition, bacterium and virus, have composition to have good control efficiency to many plant viruses, bacterium, white powder, downy mildew through testing this, environmentally safe, simultaneously due to can be medical, so its teratogenesis, carcinogenic, mutagenesis also exist; For better using the generation of this medicine delaying drug resistance, the research of its synergy proportioning is just more necessary.
Summary of the invention
The present invention is based on the every research of gliocladin in agricultural application, find and demonstrate the composition based on gliocladin, obvious at agriculture application aspect synergistic effect, have wide range of applications.
A kind of containing gliocladin composition, the composition be made up of with compd A a kind of of gliocladin or derivatives thereof, compd A is selected from one or more of following medicament: for preventing and treating the A1 of fungal disease, for preventing and treating the A2 of virus, for preventing and treating the A3 of bacterial disease, for improving crop anti-adversity to improve the A4 of crop resistance, the fertilizer composition A5 of crop growing state is improved for changing fertilizer ratio, be used for the treatment of or change environment various microorganism A6, for the plant growth regulator A7 of regulating crop growth.
The recipe ratio of gliocladin composition is by mass percentage: the one of gliocladin or derivatives thereof is 0.1:99-90:1 with compd A ratio, wherein: a kind of and compd A 1 of gliocladin or derivatives thereof, the composition of A2, A3, A6 are 1:0.1-1:10 than preferably; The composition of a kind of and A4 of gliocladin or derivatives thereof is than being preferably 30:1-1:1; The composition of a kind of and A5 of gliocladin or derivatives thereof is than being preferably 1:1-1:40; The composition of a kind of and A7 of gliocladin or derivatives thereof is than being preferably 40:1-1:20; Effective ingredient mass percent is in the composition 0.1%-99%.
For supporting technique scheme further, the recipe ratio of this gliocladin composition is by mass percentage: the recipe ratio of gliocladin composition is by mass percentage: the one of gliocladin or derivatives thereof and compd A are 0.1:99-90:1, wherein: a kind of and compd A 1 of gliocladin or derivatives thereof, the composition of A2, A3, A6 are 1:0.1-1:10; The composition of a kind of and A4 of gliocladin or derivatives thereof is 30:1-1:1; Compare for 1:5-1:30 with the composition of A5; The composition of a kind of and A7 of gliocladin or derivatives thereof is 20:1-1:5; Effective ingredient mass percent is in the composition 0.1%-99%.
Gliocladin and compd A can make various dosage form product: missible oil, wetting powder, water-dispersible granules, suspending agent, granule, oil suspending agent, seed coat agent, microcapsule formulations, fertilizer integrated with pesticide; Method of application can be spray, spread fertilizer over the fields, dress seed, broadcast execute, rush execute, fumigation, medicine fertile with sowing, seeding medicine with broadcasting.
The preparation method of each formulation is all conventionally prepared, and can add other customary adjuvant when preparing each formulation according to formulation needs.
The derivative of gliocladin comprises: 3, the esterified or etherificate of the hydroxyl on 6 carbon; Isosorbide-5-Nitrae carbonyl by hydroxylating or and then esterified or etherificate; Methyl on 2 N can be ethyl or propyl group.
A1 for preventing and treating fungal disease is selected from one or more in following product:
Hymexazo, fludioxonil, phonetic mould amine, pyraclostrobin, fluorine azoles bacterium, iprodione, fluoxastrobin, procymidone, the mould prestige of second, SYP-Zo48, Boscalid, carbendazim, thiophanate-methyl, ZEN 90160, amino-oligosaccharide, Fluoxastrobin, benomyl, metrafenone, ZJ0712, benzene metsulfovax, M 9834, zoxamide, isopyrazam, prothioconazoles, fenoxanil, eugenol, Famoxate (famoxadone), Flutriafol, fluopicolide, fluorine azoles bacterium acid amides, epoxiconazole, fluorine bacterium azoles, fluorine azoles ring bacterium amine, fluorine azoles bacterium aniline, Silthiopham, Cyproconazole, cyflufenamid, Hexanaphthene flusulfamide, RH-7592, captan, volution bacterium amine, Fenamidone, kresoxim-methyl, cyprodinil, Fluoxastrobin, fluorine frost azoles, thifluzamide, fosthiazate, mandipropamid, tetraconazole, penconazole, bromothalonil, metconazole, second phonetic phenol sulphur ester ester, isotianil, azoles mepanipyrim, gliocladin and substance A 1 is composite has good preventive and therapeutic effect to multiple fungal diseases such as various crop grey mold, downy mildew, epidemic disease, late blights afterwards, synergy is obvious, reduces dosage.
For prevent and treat virus A2 be selected from following one or more: Moroxydine Hydrochloride, amino-oligosaccharide, copper acetate, lentinan, pungent bacterium amine acetate, triacontanol, Imidacloprid, Acetamiprid, Diacloden, oligosaccharide, chitosan, cytosintetidemycin, Ningnanmycin.Gliocladin and A2 is composite has good preventive effect to multiple virus diseases such as flower leaf type, streak type, leaf roll types afterwards, improves control efficiency, reduces dosage.
A3 for preventing and treating bacterial disease be selected from following one or more: Zhongshengmycin, kasugarnycin, brave glue adipic acid copper, benziothiazolinone, S-Ethyl ethylthio sulfonate, sunlight mycin, zinc thiazole, thiophene bacterium ketone, TCCA (Trichloroisocyanuric acid), chlorobromide isocyanuric acid, sulfuric acid ketone.Gliocladin and A3 composite rear to rotting, soft corruption, ulcer, withered, the blue or green various bacterial disease such as withered have good control efficiency, reduce dosage, improve control efficiency.
For improve crop anti-adversity to the A4 improving crop resistance be selected from following one or more: amino-oligosaccharide, polypeptide protein, Acibenzolar, 1-methyl cyclopropene, chlorpropham, Thidiazuron, indolebutyric acid, cyclanilide, abscisic acid.Gliocladin and A4 composite after significantly improve anti-low temperature, Salt And Alkali Tolerance, the drought resistance of crop, improve the anti-adversity of crop, improve output.
For change fertilizer ratio improve crop growing state fertilizer composition A5 be selected from following one or more: the fertilizer such as trace element fertilizer and amino acid and the organic matters such as the moderate-element fertilizers such as various nitrogen, phosphorus, potash fertilizer, calcium, magnesium, silicon and iron, zinc, boron, copper, molybdenum.Described amino acid comprises: glycine, lysine, glutamic acid etc.Gliocladin improves plant growth after composite with A5 while preventing and treating soil-borne disease, plays the effect that fertilizer integrated with pesticide is easy to use, reduce the amount of labour.
Be used for the treatment of or change environment various microorganism A6 be selected from following one or more: bacillus subtilis, bacillus polymyxa, colloid bacillus cereus, bacillus amyloliquefaciens, bacillusmusilaginosiengineering.After gliocladin and the composite use of A6, edaphon environment can be changed, reduce the incidence of disease, improve utilization rate of fertilizer.
For plant long conditioning agent A7 be selected from following one or more: A7 be selected from following one or more: abscisic acid, amine acyl ester, brassin lactones, triacontanol, gibberellic acid, chlorination urea, CPPU, compound sodium nitrophenolate, growth hormone, gibberellin, ethrel, the basic element of cell division, brassinosteroid, salicylic acid, jasmonic, paclobutrazol, polyamines, methyl α-naphthyl acetate, heteroauxin, chlormequat, Prohexadione calcium.After gliocladin and the composite use of A7, the growth of adjustable crop, synergy is obvious, reduces dosage.
This composition also comprises in prior art the customary adjuvant being of value to composition synergistic effect except the compound containing above-mentioned active ingredient.
Beneficial effect of the present invention: the present invention utilizes the regulating action of gliocladin and the bactericidal activity of spectrum and the inhibitory action to mould and virus, this effective ingredient has good control efficiency to many plant viruses, bacterium, white powder, downy mildew, composite with selected composition, play synergistic effect; Due to can be medical, thus its ground toxicity and safety composite especially after advantage; Simultaneously because richness joins rear effect promoting, dosage reduces, and more meets the requirement of environmental protection and following national policy.
Accompanying drawing explanation
Fig. 1 is the schematic arrangement of gliocladin.
Embodiment
Be further described inventing the technical scheme related to below in conjunction with embodiment, to contribute to understanding of the present invention, but not as the restriction to technical scheme, except mentioning especially, component ratio is part by weight.
Embodiment 1
69% glue mould rhzomorph hymexazo sunlight mycin wetting powder, formula composition is for 100 grams of samples: gliocladin 6 Ke, hymexazo 60 grams, sunlight mycin 3 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 5 grams, lauryl sodium sulfate 2 grams, calcium lignosulfonate 10 grams, all the other are supplied with kaolin.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment is used for the control of bacterial wilt of ginger: when great Jiang sows, base is executed or poured water, and then spray in rainy season or every 10 days of high-incidence season or fill with root once, being watered concentration is 3000 times; Contrast medicament is the mould clever SP3000 of 70% evil times and 46% Kocide SD WG2000 times; This test at seeding time Ji Shi and rainy season time spray totally 4 medications; Sowing hourly water consumption is 600 kgs/acre, and later stage spray is 100 kgs/acre.Bacterial wilt of ginger control efficiency is in table 1:
Table 1
Sample Consumption Access times Preventive effect %
Embodiment 1 3000 times are watered 4 98.5
70% dislikes mould clever SP 2500 times are watered 4 80.4
46% Kocide SD WG 2500 times are watered 4 85.3
From table 1, this embodiment control efficiency on bacterial wilt of ginger is given prominence to, and play obvious synergistic effect, dosage reduces by 20%.
Embodiment 2
18% gliocladin amino-oligosaccharide suspension seed-coating agent, formula composition for 100 grams of samples: gliocladin 15 grams, amino-oligosaccharide 3 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 1 gram, xanthans 0.1 gram, aluminium-magnesium silicate 1 gram, White Carbon black 1 gram, calcium dodecyl benzene sulfonate 0.5 gram, phenethyl phenol mela mine-formaldehyde resin APEO 1.8 grams, OPEO 1.1 grams, polyvinyl alcohol 2 grams, carboxymethyl cellulose 1 gram, rose-red 1.7 grams, ethylene glycol 5 grams, water supplement 100 grams.
Manner of formulation: above-mentioned each material is dropped in still to shear and mix for 2 hours, after carry out sand milling, after sand milling, sampling detection, detects qualified rear packing and gets product.
This embodiment is used for the control of tobacco in seedling stage mosaic virus: with seeding and seedling raising after 30 grams of process, 100 jin of tobacco seed dressings, compare with 4% amino-oligosaccharide aqua, before tobacco emerges the transplanting of 3-4 leaf phase, carry out foliage-spray by contrast agents, transplant and carry out drug effect control efficiency statistics in latter 20 days.Mosaic virus control efficiency in seedling stage is in table 2:
Table 2
Sample Consumption Preventive effect %
Embodiment 2 30 grams/hundred jin seeds 97.4
4% amino-oligosaccharide aqua 400 times are watered 80.6
Clear water contrasts 30 kgs/acre 0
From table 2, this embodiment is obvious in tobacco mosaic virus control efficiency in seedling stage, plays obvious synergistic effect.
By gliocladin and amino-oligosaccharide composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 10% gliocladin suspension seed-coating agent and 2% amino-oligosaccharide aqua for contrast, tobacco mosaic virus Toxicity Determination, result shows co-toxicity coefficient (the Sun Yun lung co-toxicity coefficient method of each medicament to mosaic virus, lower with) between 137.85 ~ 192.67, wherein with 10/1 ~ 1/10 ratio between co-toxicity coefficient amount high, reach 165.38 ~ 192.67.
Embodiment 3
60% gliocladin Diacloden water-dispersible granules
Formula composition, for 100 grams of samples: gliocladin 20 grams, Diacloden 40 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 5 grams, polycarboxylate salt dispersant 8 grams, naphthalenesulfonatedispersant dispersant 5 grams, sodium carboxymethyl starch 7 grams, lauryl sodium sulfate 1 gram, 5 grams, ammonium sulfate, oxidised maize starch supplement 100 grams.
Manner of formulation: by laggard for above-mentioned each mixing of materials row air-flow crushing, pulverize after detect fineness and content, detect qualified after to add water mixing, then carry out granulation, drying by extrusion, detect qualified rear packing and get product.
This embodiment is used for tobacco mosaic virus controlling experiment: with 1% amino-oligosaccharide aqua, 24% fatty acid mixed copper sulphate aqueous emulsion, 40% Diacloden water-dispersible granules for contrast agents, within latter 20 days, first time medicine is used in transplanting, used again once every 10 days, be used in conjunction three times, within 10 days, carry out control efficiency statistics after third time medication and check.Result of the test is in table 3:
Table 3
Sample Consumption gram/acre Preventive effect
Embodiment 3 40 97.9
1% amino-oligosaccharide aqua 200 78.1
24% fatty acid mixed copper sulphate water and milk 100 80.2
40% Diacloden water-dispersible granules 50 83.5
Clear water contrasts 30 kgs/acre 0
From table 3, this embodiment is obvious to tobacco mosaic virus control efficiency, plays obvious synergistic effect, is significantly higher than market and commonly uses pesticide control.
By gliocladin and Diacloden composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 10% gliocladin water-dispersible granules and 10% Diacloden wetting powder for contrast, tobacco mosaic virus Toxicity Determination, result shows the co-toxicity coefficient of each medicament to mosaic virus between 135.15 ~ 195.23, wherein with 10/1 ~ 1/10 ratio between co-toxicity coefficient amount high, reach 155.27 ~ 195.23.
Embodiment 4
30% gliocladin 1-methyl cyclopropene microcapsule formulations
Formula composition, for 100 grams of samples: gliocladin 29 grams, 1-methyl cyclopropene 1 gram, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 10 grams, lauryl sodium sulfate 1 gram, polyvinyl alcohol 3 grams, absolute ethyl alcohol 5 grams, White Carbon black 20 grams, all the other supply with kaolin.
Manner of formulation: by for subsequent use after air-flow crushing after gliocladin, 1-methyl cyclopropene, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate, lauryl sodium sulfate, kaolin mixing, in closed container, add white carbon be cooled to-20 DEG C, then 1-methylcyclopropene gas is under agitation passed into, after 1-methyl cyclopropene low-temperature liquefaction is adsorbed by white carbon, under stirring, spray into polyvinyl alcohol ethanolic solution make its encystation, then detect after adding the mixing of materials adding again and crush early stage, detect qualified after be distributed into softgel shell, then bottling get product.
This embodiment is used for the fresh-keeping test of pears: with 1%1-methyl cyclopropene microcapsules granula medicament as a comparison, carry out fumigation use after gathering in warehouse, within after medicine 30 days and 60 days, carries out decayed fruit, scab, statistics check.Result of the test is in table 4:
Table 4
From table 4, this embodiment is obvious to the preservation of pears, plays obvious synergistic effect.
Embodiment 5
1% gliocladin 8% zinc oxide 20% potassium oxide fertilizer granular formulation, formula composition, for 100 grams of samples: gliocladin 1 gram, 8 grams of zinc oxide (providing with ZnSO4.7H2O), 20 grams of potassium oxides (providing with potassium nitrate), clay 10 grams, all the other supply with humus.
Manner of formulation: produce after above-mentioned each mixing of materials with drum comminutor (granulation dry integrated machine), detect qualified rear packing and get product.
This embodiment is used for maize sheath blight test: with spirit medicament as a comparison of soaking seed, and seed soaking spirit is dressed seed with 0.02% grain weight, and the present embodiment is sowed with seed with 20 kgs/acre simultaneously, emerges within latter 30 days and 60 days, to carry out statistics and check.Result of the test is in table 5:
Table 5
From table 5, this embodiment preventive and therapeutic effect on maize sheath blight is obvious, plays obvious synergistic effect.Broadcast owing to being that pharmacopoeia is same, very easy to use and safety simultaneously.
By gliocladin and zinc potash fertilizer composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 10% gliocladin granule and zinc sulphate and potassium nitrate for contrast, Rhizoctonia solani Kahn Toxicity Determination, result shows the co-toxicity coefficient of each medicament to sheath blight fungus between 132.46 ~ 172.25, wherein with 1/1 ~ 1/40 ratio between co-toxicity coefficient amount high, reach 155.44 ~ 172.25.
Embodiment 6
Gliocladin bacillus subtilis granule, formula composition, for 100 grams of samples: gliocladin 40 grams, 20,000,000,000/gram bacillus subtilis 40 grams, clay 10 grams, all the other dry with earthworm and supply.
Manner of formulation: produce after above-mentioned each mixing of materials with drum comminutor (granulation dry integrated machine), detect qualified rear packing and get product.
This embodiment is used for corn controlling disease and reduces fertilizer amount test: this test employing kind of medicine (can with fertilizer with broadcasting with broadcasting, also can spread fertilizer over the fields), consumption 15 kgs/acre, NPK fertilizer amount is reduced: reduce by 25 kgs/acre using consumption in base manure, in manuring late, reduce topdressing amount is 10 kgs/acre compared with blank; Other management examination contrasts just the same with control in vain.Each process is 1 mu of ground, if three repetitions.Within latter 20 days and 40 days, respectively investigating incidence in emerging, during results, adopting random sampling output condition.Result of the test is in table 6:
Table 6
From table 6, this is executed example and reduces fertilizer amount, reduces the incidence of disease in vegetative period, improves the output of crop simultaneously, and result of use is given prominence to.
Embodiment 7
50% gliocladin Prohexadione calcium granule, formula composition, for 100 grams of samples: gliocladin 30 grams, Prohexadione calcium 20 grams, clay 10 grams, potassium humate 20 grams, all the other supply with wormcast.
Manner of formulation: produce after above-mentioned each mixing of materials with drum comminutor (granulation dry integrated machine), detect qualified rear packing and get product.
This embodiment is for Yield Increase In Cotton comparative trial: the present embodiment 10 kgs/acre is used in this test simultaneously when cotton uses base manure, take clear water as blank; Plot area 60m 2, repeat 3 times, other way to manage with contrast completely the same.Random sampling carries out incidence investigation respectively at seedling stage, flower bud phase and flowering and boll-setting period, and add up every basal munure of cotton, Single boll weight in harvest time, result of the test is in table 7:
Table 7
From table 7, use the incidence of disease that said composition obviously can reduce cotton strain vegetative period, with fertilizer with executing, easy to use, obvious to Yield Increase In Cotton, result of use is given prominence to.
Embodiment 8
10% gliocladin Zhongshengmycin wetting powder, formula composition, for 100 grams of samples: gliocladin 7 grams, Zhongshengmycin 3 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 5 grams, lauryl sodium sulfate 1 gram, calcium lignosulfonate 7 grams, potassium dihydrogen phosphate 30 grams, all the other supply with calcium carbonate.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment is used for the control of pepper ralstonia solanacearum: carry out being watered filling root in morbidity early stage or initial stage, then every medication in 7 days once, be used in conjunction three times, with 3% Zhongshengmycin can wet-milling for contrast; Often be treated to 20m 2, establish three repetitions altogether.Last medication 10 days " Invest, Then Investigate " incidences of disease.Control efficiency is in table 8:
Table 8
Sample Preparation consumption Access times Incidence of disease %
Embodiment 8 20 grams/15 kg of water 4 0.12
3% Zhongshengmycin can wet-milling 30 grams/15 kg of water 4 8.75
Clear water contrasts 15 kg of water 4 35.5
From table 8, this embodiment control efficiency on pepper ralstonia solanacearum is given prominence to, and plays obvious synergistic effect.
By gliocladin and Zhongshengmycin composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 10% gliocladin can wet-milling and 5% Zhongshengmycin can wet-milling for contrast, tobacco mosaic virus Toxicity Determination, result shows co-toxicity coefficient (the Sun Yun lung co-toxicity coefficient method of each medicament to mosaic virus, lower with) between 137.85 ~ 192.67, wherein with 10/1 ~ 1/10 ratio between co-toxicity coefficient amount high, reach between 165.38 ~ 192.67.
Embodiment 9
25% gliocladin pyraclostrobin missible oil, formula composition, for 100 grams of samples: gliocladin 5 grams, pyraclostrobin 20 grams, alkylphenol polyoxyethylene 5 grams, fatty alcohol-polyoxyethylene ether 5 grams, phenethyl phenol formaldehyde acid ester polyoxyethylene ether 1 gram, calcium dodecyl benzene sulfonate 4 grams, ring ethyl ketone 5 grams, N dimethyl pyrrolidone 10 grams, all the other C10 solvents supply 100 grams.
Manner of formulation: mixing dissolving in above-mentioned each material input mixing kettle, filtering after dissolving, sampling detection after filtration, detect qualified rear packing and get product.
This embodiment is used for the control of powdery mildew of cucumber: carry out at the initial stage of a disease being watered spraying, then every medication in 10 days once, with 25% pyraclostrobin missible oil for contrast; Often be treated to 20m 2, establish three repetitions altogether.Last medication 5 days " Invest, Then Investigate "s prevent and treat situation.Control efficiency is in table 9:
Table 9
Sample Preparation consumption Access times Preventive effect %
Embodiment 9 30 grams/acre 2 94.2
25% pyraclostrobin missible oil 40 grams/acre 2 85.3
Clear water contrasts 30 kg of water/mu 2 0
From table 9, this embodiment control efficiency in powdery mildew of cucumber is given prominence to, and reduces dosage simultaneously; Play obvious synergistic effect.
By gliocladin and pyraclostrobin composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 5% gliocladin missible oil and 25% pyraclostrobin missible oil for contrast, powdery mildew of cucumber Toxicity Determination, result shows the co-toxicity coefficient of each medicament to Powdery Mildew between 155.81 ~ 215.79, wherein with 10/1 ~ 1/10 ratio between co-toxicity coefficient amount high, reach 184.36 ~ 215.79.
Embodiment 10
50% gliocladin Fluoxastrobin WG, formula composition, for 100 grams of samples: gliocladin 15 grams, Fluoxastrobin 35 grams, polycarboxylate salt dispersant 10 grams, naphthalenesulfonatedispersant dispersant 5 grams, sodium carboxymethyl starch 5 grams, lauryl sodium sulfate 1 gram, 15 grams, ammonium sulfate, oxidised maize starch supplement 100 grams.
Manner of formulation: by above-mentioned each mixing of materials laggard row air-flow crushing, pulverize after detect fineness and content qualified after to add water mixing, then carry out granulation, drying by extrusion, detect qualified packing and get product.
This embodiment is used for the control of rice sheath blight disease: rice tillering contains the phase and paddy rice Feng Hangqian (banded sclerotial blight is not fallen ill or their early stage temporarily) carries out being watered spraying, then in rice tillering latter stage and paddy rice Feng Hanghou medication once, before heading, dispenser in 7-10 days once, Qi Suihou executes once again, hangs for contrast with 25% Fluoxastrobin water; Often be treated to 20m 2, establish three repetitions altogether.Last medication 10 days " Invest, Then Investigate "s prevent and treat situation.Control efficiency is in table 10
Table 10
Sample Preparation consumption Access times Preventive effect %
Embodiment 10 60 grams/acre 4 93.7
25% Fluoxastrobin water hangs 100 grams/acre 4 82.8
Clear water contrasts 30 kg of water/mu 4 0
From table 10, this embodiment control efficiency in rice sheath blight disease is given prominence to, and reduces dosage simultaneously; Play obvious synergistic effect.
By gliocladin and Fluoxastrobin composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 50% gliocladin water-dispersible granules and 50% Fluoxastrobin water-dispersible granules for contrast, carry out rice sheath blight disease Toxicity Determination, result shows the co-toxicity coefficient of each medicament to Rhizoctonia solani Kuhn between 154.74 ~ 203.61, wherein with 10/1 ~ 1/10 ratio between co-toxicity coefficient amount high, reach between 179.83 ~ 203.61.
Embodiment 11
6% gliocladin abscisic acid water-dispersible granules, raw material forms: 5% gliocladin, 1% abscisic acid derivative; 0.5% calcium dodecyl benzene sulfonate, 3% naphthalenesulfonate formaldehyde condensation compound, 8% wooden calcium, 25% white vitriol, 15% potassium dihydrogen phosphate, all the other are supplied with corn starch, and extruder grain mode is produced routinely.
Improve the Saline alkali tolerance test of corn by said composition: laboratory adopts basin to carry, and waters with 0.5% sodium chloride solution and 0.5% metabisulfite solution, salinization of soil persecution process, every 3-5 days waters salt solution persecution.Processed group uses said composition 15 kgs/acre (consumption converts), spreads fertilizer over the fields once when once watering before saline and alkaline persecution, carries out saline and alkaline persecution after 3 days, and a said composition is used in every saline and alkaline persecution for 2 times.Space management is produced suppressed persecution 3 ensuing crops, occurs wilting after the 5th time, and produces normal when using the process the 5th time of said composition, and when the 7th time, just performance production is suppressed.Illustrate that said composition can significantly improve the salt resistance of crop.
Embodiment 12
80% gliocladin benzene metsulfovax wetting powder, formula composition, for 100 grams of samples: gliocladin 20 grams, benzene metsulfovax 60 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 5 grams, lauryl sodium sulfate 2 grams, alkylnaphthalene sulfonate 5 grams, all the other are supplied with calcium carbonate.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment is used for the control of downy mildew of garpe: carry out being watered spraying in morbidity early stage or initial stage, then every medication in 10 days once, be used in conjunction 3 times, with 60% benzene metsulfovax can wet-milling for contrast; Often be treated to 20m 2, establish three repetitions altogether.Last medication 20 days " Invest, Then Investigate "s prevent and treat situation.Control efficiency is in table 11:
Table 11
Sample Preparation consumption Access times Preventive effect %
Embodiment 12 20 grams/acre 3 95.2
60% benzene metsulfovax can wet-milling 30 grams/acre 3 84.1
Clear water contrasts 30 kg of water/mu 3 0
From table 11, this embodiment control efficiency on cucumber downy mildew is given prominence to, and reduces dosage simultaneously; Play obvious synergistic effect.
By gliocladin and benzene metsulfovax composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 10% gliocladin can wet-milling 60% benzene metsulfovax can wet-milling for contrast, carry out downy mildew of garpe melon Toxicity Determination, result shows the co-toxicity coefficient of each medicament to Pseudoperonospora cubensis between 150.12 ~ 211.05, wherein with 10/1 ~ 1/10 ratio between co-toxicity coefficient amount high, reach 178.68 ~ 211.05.
Embodiment 13
25% gliocladin fenoxanil suspending agent, formula composition, for 100 grams of samples: gliocladin 5 grams, fenoxanil 20 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 1 gram, calcium dodecyl benzene sulfonate 1 gram, alkylphenol polyoxyethylene 1 gram, phenethyl phenolformaldehyde resin APEO 1 gram, styrylphenol formaldehyde resin PULLRONIC F68 block type polyethers 1 gram, ethylene glycol 5 grams, aluminium-magnesium silicate 1 gram, white carbon 2 grams, xanthans 0.15 gram, all the other are supplied with water.
Manner of formulation: above-mentioned each material to be dropped in mixing kettle shear-mixed half an hour, mix rear sand milling and pulverize, after sand milling, sampling detects, and detects qualified rear packing and gets product.
This embodiment is used for the control of rice blast: in paddy rice cut initial stage and each dispenser of full heading time once, with 20% fenoxanil suspending agent for contrast; Often be treated to 20m 2, establish three repetitions altogether.Last medication 20 days " Invest, Then Investigate "s prevent and treat situation.Control efficiency is in table 12:
Table 12
Sample Preparation consumption Access times Preventive effect %
Embodiment 13 50 grams/acre 2 96.7
20% fenoxanil suspending agent 70 grams/acre 2 85.5
Clear water contrasts 30 kg of water/mu 2 0
From table 12, this embodiment control efficiency on rice blast is given prominence to, and reduces dosage simultaneously; Play obvious synergistic effect.
By gliocladin and fenoxanil composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 10% gliocladin suspending agent and 20% fenoxanil suspending agent for contrast, carry out rice blast fungus Toxicity Determination, result shows the co-toxicity coefficient of each medicament to rice blast between 162.92 ~ 218.56, wherein with 10/1 ~ 1/10 ratio between co-toxicity coefficient amount high, reach 180.05 ~ 218.56.
Embodiment 14
5% gliocladin tetraconazole aqueous emulsion, formula composition, for 100 grams of samples: gliocladin 1 gram, tetraconazole 4 grams, dimethylbenzene 20 grams, cyclohexanone 10 grams, alkylphenol polyoxyethylene 5 grams, phenethyl phenolformaldehyde resin APEO 2 grams, ethylene glycol 4 grams, all the other are supplied with water.
Manner of formulation: above-mentioned each former medicine, solvent, emulsifier to be added in mixing kettle shear-mixed half an hour, then add water under shearing condition, high speed shear sampled detection after 1 hour, detected qualified rear packing and got product.
This embodiment is used for the control of wheat sharp eyespot: spray once at wheat during jointing stage, execute once after 10 days again, with 4% tetraconazole aqueous emulsion for contrast; Often be treated to 20m 2, establish three repetitions altogether.Last medication 20 days " Invest, Then Investigate "s prevent and treat situation.Control efficiency is in table 13:
Table 13
Sample Preparation consumption Access times Preventive effect %
Embodiment 14 70 grams/acre 2 95.5
4% tetraconazole aqueous emulsion 100 grams/acre 2 82.3
Clear water contrasts 30 kg of water/mu 2 0
From table 13, this embodiment control efficiency on wheat sharp eyespot is given prominence to, and reduces dosage simultaneously; Play obvious synergistic effect.
By gliocladin and tetraconazole composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 10% gliocladin suspending agent and 4% tetraconazole aqueous emulsion for contrast, carry out rhizoctonia cerealis Toxicity Determination, result shows the co-toxicity coefficient of each medicament to wheat sharp eyespot between 153.07 ~ 195.47, wherein with 10/1 ~ 1/10 ratio between co-toxicity coefficient amount high, reach 160.86 ~ 195.47.
Embodiment 15
5% gliocladin imidacloprid soluble liquor, formula composition, for 100 grams of samples: gliocladin 1 gram, Imidacloprid 4 grams, N methyl pyrrolidone 10 grams, dimethyl formamide 10 grams, 20 grams, acetone, alkylphenol polyoxyethylene 5 grams, calcium dodecyl benzene sulfonate 2 grams, the anti-alcohol APEO of fat 2 grams, this aldehyde of phenethyl phenol first resin APEO 2 grams, ethyl acetate supply 100 grams.
Manner of formulation: above-mentioned each material is dropped into reactor, filters after stirring and dissolving, detect qualified rear packing and get product.
This embodiment is used for tomato prevention and cure of viruses test: wet-milling and 24% fatty acid mixed copper sulphate aqueous emulsion can compare medicament with 6% sulfuric acid ketone triacontanol, within latter 20 days, first time medicine is used in transplanting, used again once every 10 days, be used in conjunction three times, within 20 days, carry out control efficiency statistics after third time medication and check.Result of the test is in table 14:
Table 14
Sample Consumption gram/acre Preventive effect
Embodiment 15 100 96.7
6% sulfuric acid ketone triacontanol can wet-milling 300 85.2
24% fatty acid mixed copper sulphate water and milk 100 79.5
Clear water contrasts 30 kgs/acre 0
From table 14, this embodiment, to tomato virus disease control successful, plays obvious synergistic effect, is significantly higher than market and commonly uses pesticide control.
By gliocladin and Imidacloprid composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 10% gliocladin water-dispersible granules and 10% imidacloprid wettable powder for contrast, tomato mosaic virus viral disease Toxicity Determination, result shows the co-toxicity coefficient of each medicament to tomato virus between 140.11 ~ 192.21, wherein with 10/1 ~ 1/10 ratio between co-toxicity coefficient amount high, reach 151.38 ~ 192.21.
Embodiment 16
80% gliocladin triacontanol wetting powder, formula composition, for 100 grams of samples: gliocladin 20 grams, triacontanol 60 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 4 grams, lauryl sodium sulfate 3 grams, alkylnaphthalene sulfonate 6 grams, all the other are supplied with calcium carbonate.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment is used for tomato prevention and cure of viruses test: can compare medicament by wet-milling with 6% sulfuric acid ketone triacontanol, within latter 20 days, uses first time medicine, use once again, be used in conjunction three times every 10 days in transplanting, within 20 days, carries out control efficiency statistics and check after third time medication.Result of the test is in table 15:
Table 15
Sample Consumption gram/acre Preventive effect
Embodiment 16 20 96.7
6% sulfuric acid ketone triacontanol can wet-milling 300 84.6
Clear water contrasts 30 kgs/acre 0
From table 15, this embodiment, to tomato virus disease control successful, plays obvious synergistic effect, is significantly higher than market and commonly uses pesticide control.
By gliocladin and triacontanol composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 10% gliocladin water-dispersible granules and 10% triacontanol wetting powder for contrast, tomato mosaic virus viral disease Toxicity Determination, result shows the co-toxicity coefficient of each medicament to tomato virus between 135.57 ~ 185.69, wherein with 10/1 ~ 1/10 ratio between co-toxicity coefficient amount high, reach 146.39 ~ 185.69.
Embodiment 17
30% gliocladin lentinan wetting powder, formula composition, for 100 grams of samples: gliocladin 25 grams, lentinan 5 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 4 grams, lauryl sodium sulfate 2 grams, alkylnaphthalene sulfonate 4 grams, sodium lignin sulfonate 7 grams, all the other are supplied with calcium carbonate.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment is used for tomato prevention and cure of viruses test: compare medicament with 0.5% lentinan aqua, within latter 20 days, uses first time medicine, use once again, be used in conjunction three times every 10 days in transplanting, within 20 days, carries out control efficiency statistics and check after third time medication.Result of the test is in table 16:
Table 16
Sample Consumption gram/acre Preventive effect
Embodiment 17 20 96.9
0.5% lentinan aqua 400 81.2
Clear water contrasts 30 kgs/acre 0
From table 16, this embodiment, to tomato virus disease control successful, plays obvious synergistic effect, is significantly higher than market and commonly uses pesticide control.
By gliocladin and lentinan composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 10% gliocladin water-dispersible granules and 0.5% lentinan aqua for contrast, tomato mosaic virus viral disease Toxicity Determination, result shows the co-toxicity coefficient of each medicament to tomato virus between 145.78 ~ 192.37, wherein with 10/1 ~ 1/10 ratio between co-toxicity coefficient amount high, reach 159.47 ~ 192.37.
Embodiment 18
60% gliocladin TCCA (Trichloroisocyanuric acid) wetting powder, formula composition, for 100 grams of samples: gliocladin 10 grams, TCCA (Trichloroisocyanuric acid) 50 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 5 grams, lauryl sodium sulfate 2 grams, alkylnaphthalene sulfonate 3 grams, sodium lignin sulfonate 10 grams, all the other are supplied with kaolin.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment is used for pepper anthracnose controlling experiment: can compare medicament by wet-milling with 42% TCCA (Trichloroisocyanuric acid), in time transplanting, execute first time medicine, transplant and use second time medicine in latter 10 days, used again once every 10 days, be used in conjunction three times, within 20 days, carry out control efficiency statistics after third time medication and check.Result of the test is in table 17
Table 17
Sample Consumption gram/acre Incidence of disease %
Embodiment 18 100 0.15
42% TCCA (Trichloroisocyanuric acid) can wet-milling 200 4.12
Clear water contrasts 30 kgs/acre 10
From table 17, this embodiment is obvious to pepper anthracnose control efficiency, plays obvious synergistic effect, is significantly higher than market and commonly uses pesticide control.
Embodiment 19
30% gliocladin S-Ethyl ethylthio sulfonate missible oil, formula composition, for 100 grams of samples: gliocladin 5 grams, S-Ethyl ethylthio sulfonate missible oil 25 grams, alkylphenol polyoxyethylene 4 grams, fatty alcohol-polyoxyethylene ether 2 grams, phenethyl phenol formaldehyde acid ester polyoxyethylene ether 2 grams, calcium dodecyl benzene sulfonate 4 grams, ring ethyl ketone 5 grams, N dimethyl pyrrolidone 10 grams, 10 grams, acetone, all the other C10 solvents supply 100 grams.
Manner of formulation: mixing dissolving in above-mentioned each material input mixing kettle, filtering after dissolving, sampling detection after filtration, detect qualified rear packing and get product.
This embodiment is used for the control of cotton wilt: carry out being watered spray in morbidity early stage or initial stage, then every 10 days again medication once, with 30% S-Ethyl ethylthio sulfonate missible oil for contrast; Often be treated to 20m 2, establish three repetitions altogether.Last medication 15 days " Invest, Then Investigate "s prevent and treat situation.Control efficiency is in table 18:
Table 18
Sample Preparation consumption Access times Incidence of disease %
Embodiment 19 80 grams/acre 2 0.43
30% S-Ethyl ethylthio sulfonate missible oil 100 grams/acre 2 3.79
Clear water contrasts 60 kg of water/mu 2 10.95
From table 18, this embodiment control efficiency on cotton wilt is given prominence to, and reduces dosage simultaneously; Play obvious synergistic effect.
Embodiment 20
5% gliocladin benziothiazolinone aqueous emulsion, formula composition, for 100 grams of samples: gliocladin 4 grams, benziothiazolinone 1 gram, dimethylbenzene 25 grams, cyclohexanone 10 grams, alkylphenol polyoxyethylene 5 grams, phenethyl phenolformaldehyde resin APEO 3 grams, ethylene glycol 5 grams, all the other are supplied with water.
Manner of formulation: above-mentioned each former medicine, solvent, emulsifier to be added in mixing kettle shear-mixed half an hour, then add water under shearing condition, high speed shear sampled detection after 1 hour, detected qualified rear packing and got product.
This embodiment is used for the control of ring rot of apple: spray once at premorbid or initial stage, then execute once after 15 days again, be used in conjunction three times, with 1.5% benziothiazolinone aqueous emulsion for contrast; Often be treated to 20m 2, establish three repetitions altogether.Last medication 15 days " Invest, Then Investigate "s prevent and treat situation.Control efficiency is in table 19:
Table 19
Sample Preparation consumption Access times Preventive effect %
Embodiment 20 60 grams/acre 3 94.2
1.5% benziothiazolinone aqueous emulsion 200 grams/acre 3 80.3
Clear water contrasts 30 kg of water/mu 3 0
From table 19, this embodiment control efficiency in ring rot of apple is given prominence to, and reduces dosage simultaneously; Play obvious synergistic effect.
Embodiment 21
20% gliocladin benziothiazolinone suspending agent
Formula composition, for 100 grams of samples: gliocladin 10 grams, benziothiazolinone 10 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 1 gram, calcium dodecyl benzene sulfonate 1.5 grams, alkylphenol polyoxyethylene 1 gram, phenethyl phenolformaldehyde resin APEO 1.5 grams, styrylphenol formaldehyde resin PULLRONIC F68 block type polyethers 1.5 grams, ethylene glycol 5 grams, aluminium-magnesium silicate 1.5 grams, white carbon 2 grams, xanthans 0.15 gram, all the other are supplied with water.
Manner of formulation: above-mentioned each material to be dropped in mixing kettle shear-mixed half an hour, mix rear sand milling and pulverize, after sand milling, sampling detects, and detects qualified rear packing and gets product.
This embodiment is used for the control of bacterial leaf streak of rice: in premorbid or initial stage dispenser once, every dispenser in 10 days once, connect and execute 3 times, with 5% benziothiazolinone suspending agent for contrast; Often be treated to 20m 2, establish three repetitions altogether.Last medication 15 days " Invest, Then Investigate "s prevent and treat situation.Control efficiency is in table 20:
Table 20
Sample Preparation consumption Access times Preventive effect %
Embodiment 21 20 grams/acre 3 97.8
5% benziothiazolinone suspending agent 100 grams/acre 3 83.2
Clear water contrasts 30 kg of water/mu 3 0
From table 20, this embodiment control efficiency in paddy bacterial bar class disease is given prominence to, and reduces dosage simultaneously; Play obvious synergistic effect.
Embodiment 22
10% gliocladin chlorpropham wetting powder
Formula composition, for 100 grams of samples: gliocladin 9 grams, chlorpropham 1 gram, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 4 grams, lauryl sodium sulfate 2 grams, alkylnaphthalene sulfonate 2 grams, sodium lignin sulfonate 11 grams, all the other are supplied with kaolin.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment effect when testing for the low temperature resistant process of tomato seedling is as follows: be watered with 2000 times shortly before low temperature occurs and spray said composition, can significantly improve the freezing tolerance of tomato seedling.Be blank with water spray and spray same multiple 10% gliocladin can wet-milling and 2% chlorpropham can wet-milling for contrasting, verify the performance of said composition.0-5 DEG C of low temperature treatment is carried out after spraying 12 hours, detect soluble sugar and soluble protein in three kinds of lower seedling of process after low temperature treatment after 48 hours respectively: clear water be: 0.4902 μ g and 0.3148 μ g, what gliocladin contrasted is: 0.6125 μ g and 0.3031 μ g, what chlorpropham contrasted is: 0.6003 μ g and 0.3002 μ g, this invention composition be: 0.7949 μ g and 0.2662 μ g, spray as can be seen here said composition can significantly improve soluble sugar in seedling body amount and reduce soluble protein amount thus improve crop to the tolerance of low temperature.
Embodiment 23
21% gliocladin indolebutyric acid wetting powder
Formula composition, for 100 grams of samples: gliocladin 20 grams, indolebutyric acid 1 gram, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 5 grams, lauryl sodium sulfate 2 grams, sodium lignin sulfonate 12 grams, all the other are supplied with kaolin.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment is used for increasing production of rice comparative trial: with 10% gliocladin wetting powder and 1% indolebutyric acid wetting powder for contrast agents, be watered spraying, take clear water as blank at rice tillering after date; Plot area 20m 2, repeat 3 times.Add up the thousand kernel weight of random sampling harvest time to paddy rice, result table 21:
Table 21
From table 21, after said composition process, paddy rice thousand kernel weight and number of grain per ear process apparently higher than other, obvious to paddy growth regulating action, to increasing production of rice successful.
Embodiment 24
66% gliocladin many too albumen WG
Formula composition, for 100 grams of samples: gliocladin 60 grams, polypeptide protein 6 grams, polycarboxylate salt dispersant 8 grams, naphthalenesulfonatedispersant dispersant 4 grams, sodium carboxymethyl starch 6 grams, lauryl sodium sulfate 1 gram, 10 grams, ammonium sulfate, oxidised maize starch supplement 100 grams.
Manner of formulation: by above-mentioned each mixing of materials laggard row air-flow crushing, pulverize after detect fineness and content qualified after to add water mixing, then carry out granulation, drying by extrusion, detect qualified packing and get product.
This embodiment is used for wheat increase yield comparative trial: with 60% gliocladin WG and 6% polypeptide protein wetting powder for contrast agents, be watered spraying, take clear water as blank at wheat tillering after date; Plot area 20m 2, repeat 3 times.In harvest time, random sampling is added up thousand grain weight of wheat, result table 22:
Table 22
From table 22, after said composition process, thousand grain weight of wheat processes apparently higher than other, obvious to corn growth regulating action, to corn yield increasing successful.
Embodiment 25
27% gliocladin 15% silica 1 0% potassium oxide wetting powder, formula composition, for 100 grams of samples: gliocladin 2 grams, 15 grams of silica (providing silicon source with Water-soluble silicon fertilizer), 10 grams of potassium oxides (providing with potassium nitrate), di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 5 grams, lauryl sodium sulfate 1 gram, alkylnaphthalene sulfonate 10 grams, all the other are supplied with potash.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment is used for increasing production of rice comparative trial: with 10% gliocladin wetting powder, be watered spraying at rice tillering after date, executed once every 20 days again, take clear water as blank; Plot area 20m 2, repeat 3 times.In random sampling harvest time, the incidence of disease of paddy rice and thousand kernel weight are added up, result table 23:
Table 23
From table 23, after said composition process, rice pathogenesis rate reduces, and paddy rice thousand kernel weight and number of grain per ear process apparently higher than other, obvious to paddy growth regulating action, to increasing production of rice successful.
Embodiment 26
21% gliocladin amino acid wetting powder
Formula composition, for 100 grams of samples: gliocladin 19 grams, glycine 1 gram, lysine 0.6 gram, 0.4 gram, glutamic acid, ferrous sulfate heptahydrate 3 grams, white vitriol 2 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 4 grams, lauryl sodium sulfate 3 grams, sodium lignin sulfonate 14 grams, all the other are supplied with kaolin.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment is used for wheat increase yield comparative trial: with 10% gliocladin wetting powder for contrast agents, executes first time medicine at period of seedling establishment, and full heading time is executed second time medicine, is blank with clear water; Plot area 20m 2, repeat 3 times.Add up the thousand kernel weight of random sampling harvest time to wheat, result table 24:
Table 24
From table 24, process apparently higher than other with thousand grain weight of wheat and number of grain per ear after said composition process, obvious to wheat growth regulating action, to wheat increase yield successful.
Embodiment 27
10% gliocladin 100,000,000 EFU/ gram bacillus amyloliquefaciens wetting powder, formula composition, for 100 grams of samples: gliocladin 10 grams, bacillus amyloliquefaciens 20 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 7 grams, lauryl sodium sulfate 2 grams, calcium lignosulfonate 7 grams, alkylnaphthalene sulfonate 8 grams, all the other supply with calcium carbonate.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment is used for the control of rice blast: in paddy rice cut initial stage and each dispenser of full heading time once, with 10% gliocladin wettable powder for contrast; Often be treated to 20m 2, establish three repetitions altogether.Last medication 20 days " Invest, Then Investigate "s prevent and treat situation.Control efficiency is in table 25:
Table 25
Sample Preparation consumption Access times Preventive effect %
Embodiment 27 30 grams/acre 2 94.4
10% gliocladin wettable powder 80 grams/acre 2 81.1
Clear water contrasts 30 grams/acre 2 0
From table 25, this embodiment control efficiency on rice blast is given prominence to, and reduces dosage simultaneously; Play obvious synergistic effect.
Embodiment 28
2% gliocladin 1,000,000,000 EFU/ gram bacillus polymyxa granule, formula composition, for 100 grams of samples: gliocladin 2 grams, bacillus polymyxa 8 grams, clay 8 grams, potassium humate 30 grams, all the other supply with wormcast.
Manner of formulation: produce after above-mentioned each mixing of materials with drum comminutor (granulation dry integrated machine), detect qualified rear packing and get product.
This embodiment is preventing and treating the sick comparative trial of blue or green tangerine for tomato: use the present embodiment 20 kgs/acre when base manure is used in this test before field planting simultaneously, with the bacillus polymyxa of 1,000,000,000 EFU/ grams can wet-milling for contrast, take clear water as blank; Plot area 60m 2, spread fertilizer over the fields once at premorbid, other way to manage with contrast completely the same, repeat 3 times.Random sampling carries out preventive effect statistics statistics in period of disease, and result of the test is in table 26:
Table 26
Sample Incidence of disease %
Embodiment 7 0.01
The bacillus polymyxa of 1000000000 EFU/ grams can wet-milling 3.57
Clear water contrasts 9.89
From table 26, use the incidence of disease that said composition obviously can reduce tomato bacterial wilt, result of use is given prominence to.
Embodiment 29
15% gliocladin paclobutrazol water-dispersible granules, formula composition, for 100 grams of samples: gliocladin 5 grams, paclobutrazol 10 grams, polycarboxylate salt dispersant 6 grams, naphthalenesulfonatedispersant dispersant 5 grams, sodium carboxymethyl starch 8 grams, lauryl sodium sulfate 1 gram, 30 grams, ammonium sulfate, oxidised maize starch supply 100 grams.
Manner of formulation: by above-mentioned each mixing of materials laggard row air-flow crushing, pulverize after detect fineness and content qualified after to add water mixing, then carry out granulation, drying by extrusion, detect qualified packing and get product.
This embodiment is for wheat comparative trial resistant to lodging: with 10% paclobutrazol wetting powder for contrast agents, carry out between wheat tillering stage to jointing initial stage 500 times be watered spraying, take clear water as blank.Plot area 20m 2, repeat 3 times.Add up plant height, the strain footpath of wheat, the situation that lodges (to topple over 45 degree for lodging), thousand kernel weight in random sampling harvest time, result of the test is as table 27:
Table 27
From table 27, said composition obviously can reduce Plant Height in Wheat, improve footpath slightly, strengthens wheat lodging tolerance, obvious to wheat growth regulating action; Contrast to process with other with clear water and compare, said composition is to wheat increase yield successful.
Embodiment 30
10% gliocladin gibberellic acid oil-suspending agent, formula composition, to prepare 100 grams of samples: gliocladin 8 grams, gibberellic acid 2 grams, organobentonite 1 gram, gas phase White Carbon black 2 grams, sodium carboxymethyl starch 1 gram, fatty alcohol-polyoxyethylene ether 4 grams, sorbester p17 emulsifier 5 grams, polyethylene glycol dioleic acid ester 5 grams, linoleic acid LABRAFIL M 1944CS 4.5 grams, calcium dodecyl benzene sulfonate 3 grams, soybean oil supplement 100 grams.
Manner of formulation: detect after carrying out sand milling, sand milling after above-mentioned each mixing of materials, detects qualified rear packing and gets product.
This embodiment is for Resistance Strain of Cotton lodging volume increase comparative trial: be contrast agents with 10% mepiquat chloride soluble powder and 5% gliocladin wetting powder, being watered spraying at cotton initial bloom stage, take clear water as blank; Plot area 20m 2, repeat 3 times.Add up the plant height of cotton, the situation that lodges (to topple over 45 degree for lodging), every basal munure, Single boll weight in random sampling harvest time, result of the test is in table 28:
Table 28
From table 28, said composition obviously can reduce cotton plant height, strengthen lodging tolerance, obvious to cotton growth regulating action, contrasts to process with other compare said composition to Yield Increase In Cotton successful with clear water.
Embodiment 31
30% gliocladin derivative kresoxim-methyl wetting powder, formula composition, for 100 grams of samples: 10 grams, gliocladin derivative (hydroxyl on 3 and 6 carbon one is esterified as 6 by esterification), kresoxim-methyl 20 grams, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 8 grams, lauryl sodium sulfate 2 grams, sodium lignin sulfonate 10 grams, alkylnaphthalene sulfonate 10 grams, all the other supply with calcium carbonate.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment is used for the control of powdery mildew of cucumber: carry out at the initial stage of a disease being watered spraying, then every medication in 10 days once, with 30% kresoxim-methyl can wet-milling for contrast; Often be treated to 20m 2, establish three repetitions altogether.Last medication 5 days " Invest, Then Investigate "s prevent and treat situation.Control efficiency is in table 29:
Table 29
Sample Preparation consumption Access times Preventive effect %
Embodiment 31 25 grams/acre 2 94.4
30% azoles kresoxim-methyl WP 30 grams/acre 2 85.1
Clear water contrasts 30 kg of water/mu 2 0
From table 29, this embodiment control efficiency in powdery mildew of cucumber is given prominence to, and reduces dosage simultaneously; Play obvious synergistic effect.
By gliocladin derivative and kresoxim-methyl composite by a certain percentage, its ratio is: 60/1,40/1,30/1,20/1,10/1,1/1,1/10,1/20,1/30,1/40,1/60, with 10% gliocladin can wet-milling and 30% azoles kresoxim-methyl WP for contrast, powdery mildew of cucumber Toxicity Determination, result shows the co-toxicity coefficient of each medicament to Powdery Mildew between 152.13 ~ 212.67, wherein with 10/1 ~ 1/10 ratio between co-toxicity coefficient amount high, reach 183.01 ~ 212.67.
Embodiment 32
4% gliocladin derivative fludioxonil granule, formula composition, for 100 grams of samples: gliocladin derivative (1,4 carbonyls can by hydroxylating or and then esterified or etherificate, this embodiment be 1 by ether) 2 grams, fludioxonil 2 grams, wormcast 30 grams, clay 1 gram of colloid bacillus cereus 2 grams, clay 10 grams, all the other supply with potassium humate.
Manner of formulation: produce after above-mentioned each mixing of materials with drum comminutor (granulation dry integrated machine), detect qualified rear packing and get product.
This embodiment is used for root rotof flax controlling experiment: with 4% fludioxonil granule for contrast agents, and the present embodiment is sowed with seed with 20 kgs/acre simultaneously, emerges within latter 30 days and 90 days, to carry out statistics and check.Result of the test is in table 30:
Table 30
From table 30, this embodiment preventive and therapeutic effect in root rotof flax is obvious, plays obvious synergistic effect.Broadcast owing to being that pharmacopoeia is same, very easy to use and safety simultaneously.
Embodiment 33
15% gliocladin derivative zinc thiazole 12%P 2o 5206%K 2o wetting powder, formula composition, for 100 grams of samples: (its derivative is the methyl on 2 N can be ethyl or propyl group to gliocladin derivative, what this was routine is ethyl) 5 grams, zinc thiazole 10 grams, 12 grams of phosphorus pentoxides (providing with potassium dihydrogen phosphate), 6 grams of potassium oxides (providing with potassium dihydrogen phosphate and potassium nitrate), di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate 6 grams, lauryl sodium sulfate 1 gram, calcium lignosulfonate 5 grams, alkylnaphthalene sulfonate 10 grams, all the other supply with potassium humate.
Manner of formulation: above-mentioned each material is dropped in mixing kettle and mixes half an hour, mix laggard row air-flow crushing, sample detection after pulverizing, detect qualified rear packing and get product.
This embodiment is used for the control of cucumber bacterial angular leaf spot: in morbidity early stage or initial stage dispenser once (spraying), every 7 days, medication once, was used in conjunction 3 times again, with 10% zinc thiazole wettable powder for contrasting; Often be treated to 20m 2, establish three repetitions altogether.Last medication 7 days " Invest, Then Investigate "s prevent and treat situation.Control efficiency is in table 31:
Table 31
Sample Preparation consumption Access times Preventive effect %
Embodiment 27 100 grams/acre 3 93.9
20% zinc thiazole wettable powder 150 grams/acre 3 79.8
Clear water 30 kg of water/mu 3 0
From table 31, this embodiment control efficiency on cucumber bacterial angular leaf spot is given prominence to, and reduces dosage simultaneously; Play obvious synergistic effect.

Claims (10)

1., containing the composition of gliocladin, its active ingredient is made up of the one of gliocladin or derivatives thereof and compd A, it is characterized in that:
Compd A is selected from one or more of following medicament: for preventing and treating the A1 of fungal disease, for preventing and treating the A2 of virus, for preventing and treating the A3 of bacterial disease, for improving crop anti-adversity to improve the A4 of crop resistance, the fertilizer composition A5 of crop growing state is improved for changing fertilizer ratio, be used for the treatment of or change environment various microorganism A6, for the plant growth regulator A7 of regulating crop growth;
The recipe ratio of composition active ingredient is by weight: the one of gliocladin or derivatives thereof and compd A than being 0.1:99-90:1, wherein: a kind of and compd A 1 of gliocladin or derivatives thereof, the composition of A2, A3, A6 are 1:0.1-1:10 than preferably; The composition of a kind of and A4 of gliocladin or derivatives thereof is than being preferably 30:1-1:1; The composition of a kind of and A5 of gliocladin or derivatives thereof is than being preferably 1:1-1:40; The composition of a kind of and A7 of gliocladin or derivatives thereof is than being preferably 40:1-1:20; Effective ingredient mass percent is in the composition 0.1%-99%.
2. composition according to claim 1, it is characterized in that: the recipe ratio of gliocladin composition by weight: the one of gliocladin or derivatives thereof and compd A are 0.1:99-90:1, wherein: a kind of and compd A 1 of gliocladin or derivatives thereof, the composition of A2, A3, A6 are 1:0.1-1:10; The composition of a kind of and A4 of gliocladin or derivatives thereof is 30:1-1:1; Compare for 1:5-1:30 with the composition of A5; The composition of a kind of and A7 of gliocladin or derivatives thereof is 20:1-1:5; Effective ingredient mass percent is in the composition 0.1%-99%.
3. composition according to claim 1, is characterized in that: the derivative of gliocladin comprises: 3, the esterified or etherificate of the hydroxyl on 6 carbon; Isosorbide-5-Nitrae carbonyl by hydroxylating or and then esterified or etherificate; Methyl on 2 N can be ethyl or propyl group.
4. composition according to claim 1, is characterized in that: A1 is selected from one or more the: hymexazos in following product, fludioxonil, phonetic mould amine, pyraclostrobin, fluorine azoles bacterium, iprodione, fluoxastrobin, procymidone, the mould prestige of second, SYP-Zo48, Boscalid, carbendazim, thiophanate-methyl, ZEN 90160, amino-oligosaccharide, Fluoxastrobin, benomyl, metrafenone, ZJ0712, benzene metsulfovax, M 9834, zoxamide, isopyrazam, prothioconazoles, fenoxanil, eugenol, Famoxate, Flutriafol, fluopicolide, fluorine azoles bacterium acid amides, epoxiconazole, fluorine bacterium azoles, fluorine azoles ring bacterium amine, fluorine azoles bacterium aniline, Silthiopham, Cyproconazole, cyflufenamid, Hexanaphthene flusulfamide, RH-7592, captan, volution bacterium amine, Fenamidone, kresoxim-methyl, cyprodinil, Fluoxastrobin, fluorine frost azoles, thifluzamide, fosthiazate, mandipropamid, tetraconazole, penconazole, bromothalonil, metconazole, second phonetic phenol sulphur ester ester, isotianil, azoles mepanipyrim.
5. composition according to claim 1, is characterized in that: A2 be selected from following one or more: Moroxydine Hydrochloride, amino-oligosaccharide, copper acetate, lentinan, pungent bacterium amine acetate, triacontanol, Imidacloprid, Acetamiprid, Diacloden, oligosaccharide, chitosan, cytosintetidemycin, Ningnanmycin.
6. composition according to claim 1, is characterized in that: A3 be selected from following one or more: Zhongshengmycin, kasugarnycin, brave glue adipic acid copper, benziothiazolinone, S-Ethyl ethylthio sulfonate, sunlight mycin, zinc thiazole, thiophene bacterium ketone, TCCA (Trichloroisocyanuric acid), chlorobromide isocyanuric acid, sulfuric acid ketone.
7. composition according to claim 1, is characterized in that: A4 be selected from following one or more: amino-oligosaccharide, polypeptide protein, Acibenzolar, 1-methyl cyclopropene, chlorpropham, Thidiazuron, indolebutyric acid, cyclanilide.
8. composition according to claim 1, is characterized in that: A5 be selected from following one or more: the fertilizer such as trace element fertilizer and amino acid and the organic matters such as the moderate-element fertilizers such as various nitrogen, phosphorus, potash fertilizer, calcium, magnesium, silicon and iron, zinc, boron, copper, molybdenum.
9. composition according to claim 1, is characterized in that: A6 be selected from following one or more: bacillus subtilis, bacillus polymyxa, colloid bacillus cereus, bacillus amyloliquefaciens, bacillusmusilaginosiengineering.
10. composition according to claim 1, is characterized in that: A7 be selected from following one or more: abscisic acid, amine acyl ester, brassin lactones, triacontanol, gibberellic acid, chlorination urea, CPPU, compound sodium nitrophenolate, growth hormone, gibberellin, ethrel, the basic element of cell division, brassinosteroid, salicylic acid, jasmonic, paclobutrazol, polyamines, methyl α-naphthyl acetate, heteroauxin, chlormequat, Prohexadione calcium.
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