CN105418783A - Bletilla striata polysaccharide extraction and purification method - Google Patents
Bletilla striata polysaccharide extraction and purification method Download PDFInfo
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- CN105418783A CN105418783A CN201510856341.3A CN201510856341A CN105418783A CN 105418783 A CN105418783 A CN 105418783A CN 201510856341 A CN201510856341 A CN 201510856341A CN 105418783 A CN105418783 A CN 105418783A
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Abstract
The invention belongs to the technical field of food and medicinal material purification, and particularly relates to a bletilla striata polysaccharide extraction and purification method. The method comprises the steps that 1, a bletilla striata polysaccharide medicinal material is dissolved through distilled water, ultrasonic extraction and filtration are conducted, and a bletilla striata polysaccharide extracting solution is obtained; 2, the bletilla striata polysaccharide extracting solution is adsorbed and purified through macroporous adsorption resin, and bletilla striata polysaccharide flow-out liquid is obtained; 3, the bletilla striata polysaccharide flow-out liquid is eluted through column volume distilled water, and bletilla striata polysaccharide eluent is obtained; 4, precipitation and concentration are conducted on the bletilla striata polysaccharide eluent through ethanol, and a bletilla striata polysaccharide purified product is obtained. According to the bletilla striata polysaccharide extraction and purification method, a traditional method is replaced with a macroporous resin method, decoloration and deproteinization can be conducted simultaneously in one time, and the bletilla striata polysaccharide extraction and purification method has the advantages of being few in technological process and low in cost. The overall purity of obtained bletilla striata polysaccharide is 85 percent or above.
Description
Technical field
The invention belongs to food, medicinal material purification techniques field, specifically, relate to a kind of BSP extracting and purifying method.
Background technology
In the bletilla striata, main component is BSP, also has the micromolecular compound such as biphenyl class and luxuriant and rich with fragrance class, in addition, also has the general character composition-albumen in a large amount of plant and starch.BSP is glucomannan, and the ratio of two kinds of glycosyls is approximately 4: 1, molecular-weight average 2800kD, and soluble in water, used water is as solvent.The separation and purification of polysaccharide generally will through links such as extraction, deproteinated, decolourings, and in the process, need use a large amount of organic solvents, as trichoroacetic acid(TCA), chloroform etc., technique is loaded down with trivial details, and the used time is longer, very easily affects polysaccharide quality and yield.The difference of extraction conditions especially Extracting temperature, has remarkably influenced to the dissolution rate of the water-soluble protein, Zulkovsky starch, pigment etc. that comprise polysaccharose substance, then causes impact in various degree to follow-up refining, the purifying process of BSP.Therefore extraction conditions selection for the yield of BSP and purity most important.If be heated beyond 80 DEG C, starch will gelatinization and a large amount of stripping, the simultaneously also together stripping of a large amount of water-soluble impurity, if lower than 60 DEG C of extractions.The rare report of purification refine document of BSP, market product is multiplex traditional method also, complex process, and purity is generally not high, and color is partially dark, and with flavour of a drug, added value of product is low, and application receives certain restriction.
Summary of the invention
For above-mentioned technical problem, the technical problem to be solved in the present invention is to provide a kind of short, safe and reliable, simple BSP extracting and purifying method of environment-friendly type, process consuming time.
In order to solve the problems of the technologies described above, technical scheme provided by the invention is: a kind of BSP extracting and purifying method, and step is:
A, dissolved by BSP medicinal material distilled water, supersound extraction filters to obtain BSP extracting solution;
B, BSP extracting solution obtain BSP effluent liquid by absorption with macroporous adsorbent resin purifying;
C, BSP effluent liquid is obtained BSP elutriant through column volume distilled water wash-out;
D, BSP elutriant is obtained BSP purified with alcohol settling is concentrated.
BSP is a kind of polyhydric neutral polysaccharide, and be the main component in bletilla striata extracting solution, generally on macroporous resin, adsorptive capacity is little.Albumen and pigment accounting relatively little, but affect the quality of polysaccharide.Replace traditional method with Amberlyst process, can simultaneously disposablely carry out decolouring, deproteinated, there is process procedure few, the feature that cost is low, use no or little organic solvent, decrease environmental pollution, and polysaccharide loss be little, drastically increase the productive rate of polysaccharide.
Further: in above-mentioned BSP extracting and purifying method, steps A refers to that first time adds BSP medicinal material 5-15 distilled water doubly, and ultrasonic 20-40 minute, second time adds BSP medicinal material 5-10 times of water, ultrasonic 20-30 minute.Steps A also comprises the suitable crude drug 0.5-1g/ml of adjustment filter liquor concentration.
Described step B refers to BSP extracting solution loading 2-5 column volume, receives effluent liquid, with 1-2 column volume distilled water wash-out.
The alcohol concn of D step is more than 95%.
Compared with prior art, the present invention adopts ultrasonic extraction, avoids the together stripping of a large amount of water-soluble impurity, use no or little organic solvent, decrease environmental pollution, and polysaccharide loss is little, drastically increase the productive rate of polysaccharide.The present invention's Amberlyst process replaces traditional method, can simultaneously disposablely carry out decolouring, deproteinated, and have process procedure few, the feature that cost is low, the overall purity of BSP of the present invention is more than 85%.
Embodiment
Be described in further detail content of the present invention below in conjunction with embodiment, content mentioned in embodiment is not limitation of the invention, and the selection of its Raw, temperature, time and rate of temperature fall can be suited measures to local conditions, and there is no substantial impact to result.
1, the process of material
Bletilla striata medicinal material 60 DEG C of oven drying at low temperatures, pulverize, and cross 20 mesh sieves for subsequent use.
2, the extraction of polysaccharide
A, medicinal material 200g, add the distilled water supersound extraction 30min of 10 times amount, and filter, filter residue adds the ultrasonic 20min of distilled water of 8 times amount again, merging filtrate, reduction vaporization moisture, regulates the suitable crude drug 0.5-1g/ml of filter liquor concentration.
B, BSP extracting solution is obtained BSP effluent liquid by absorption with macroporous adsorbent resin purifying, i.e. a BSP extracting solution loading 2-5 column volume, receive effluent liquid;
C, BSP effluent liquid is obtained BSP elutriant through column volume distilled water wash-out, namely use 1-2 column volume distilled water wash-out;
D, BSP elutriant is obtained BSP purified with alcohol settling is concentrated.
Measure the albumen in medicinal material and extracting liq and polysaccharide content, calculate extraction yield as shown in table 1;
The OD value mentioned herein is light absorption value, also can be described as extinction value.
Table 1: the content of polysaccharide, albumen in extracting solution
From experimental result, the extraction yield of polysaccharide and albumen is respectively 87.28%, 88.70%, illustrates that this extracting method is good, higher to the extraction efficiency of BSP.
Choose the absorption situation of different model resin to polysaccharide and albumen as shown in table 2:
Table 2:
From Comparison of experiment results, NKA – II and the adsorption rate of NKA – 9 resin to polysaccharide less, but comparatively large to the adsorption rate of albumen, also can qualitatively find out from the color of liquid simultaneously, after this resin absorption, liquid color shoals, and illustrate the absorption property of pigment also better, but NKA – 9 resin is in follow-up dress column test, liquid fluidity is bad, for a long time consuming time, therefore, select NKA – II resin to be optimum resin.
Claims (5)
1. a BSP extracting and purifying method, step is:
A, dissolved by BSP medicinal material distilled water, supersound extraction filters to obtain BSP extracting solution;
B, BSP extracting solution obtain BSP effluent liquid by absorption with macroporous adsorbent resin purifying;
C, BSP effluent liquid is obtained BSP elutriant through column volume distilled water wash-out;
D, BSP elutriant is obtained BSP purified with alcohol settling is concentrated.
2. BSP extracting and purifying method according to claim 1, is characterized in that: steps A refers to that first time adds BSP medicinal material 5-15 distilled water doubly, and ultrasonic 20-40 minute, second time adds BSP medicinal material 5-10 times of water, ultrasonic 20-30 minute.
3. BSP extracting and purifying method according to claim 2, is characterized in that: steps A also comprises the suitable crude drug 0.5-1g/ml of adjustment filter liquor concentration.
4. BSP extracting and purifying method according to claim 3, is characterized in that:
Described step B refers to BSP extracting solution loading 2-5 column volume, receives effluent liquid; Described step C is with 1-2 column volume distilled water wash-out.
5. BSP extracting and purifying method according to claim 4, is characterized in that: the alcohol concn of D step is more than 95%.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106397627A (en) * | 2016-11-24 | 2017-02-15 | 贵州盛茂白芨开发有限公司 | Gum bletilla medium addition extraction method |
| CN106632718A (en) * | 2016-12-28 | 2017-05-10 | 西安医学院 | Preparation method for rhizoma bletillae polysaccharide |
| CN106674370A (en) * | 2017-01-24 | 2017-05-17 | 贵州省武陵山药用植物白芨开发有限公司 | Bletilla striata gelatin and preparation method thereof |
| CN106832038A (en) * | 2017-03-30 | 2017-06-13 | 镇远县道益堂生物科技有限公司 | A kind of extracting method of bletilla polysaccharide |
| CN106883310A (en) * | 2017-04-17 | 2017-06-23 | 龙岩学院 | A kind of Deproteinated purification process of the very best beans polysaccharide decolouring |
| CN106893000A (en) * | 2017-03-16 | 2017-06-27 | 云南龙发制药股份有限公司 | A kind of Bletilla glucomannan extracting method |
| CN109364085A (en) * | 2018-10-29 | 2019-02-22 | 湖北中医药大学 | Bletilla striata oligosaccharides and combinations thereof is adjusting the application in glucose -lipid metabolism disorder |
| CN110075048A (en) * | 2019-06-03 | 2019-08-02 | 王金会 | A kind of Chinese herbal medicine cosmetic and preparation method thereof |
| CN110642960A (en) * | 2019-11-07 | 2020-01-03 | 皖西学院 | Double-aqueous-phase extraction method of bletilla striata glucomannan |
| CN111004335A (en) * | 2019-11-29 | 2020-04-14 | 湖南绿蔓生物科技股份有限公司 | Bletilla striata oligosaccharide extracted from rhizome of bletilla striata and preparation method thereof |
| CN113304088A (en) * | 2021-05-26 | 2021-08-27 | 上海辉文生物技术股份有限公司 | Preparation method of bletilla striata extract with repairing and whitening effects and bletilla striata extract |
| CN113354750A (en) * | 2021-05-31 | 2021-09-07 | 西安绿天生物技术有限公司 | Extraction method and application of bletilla striata polysaccharide with moisturizing effect |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106397627A (en) * | 2016-11-24 | 2017-02-15 | 贵州盛茂白芨开发有限公司 | Gum bletilla medium addition extraction method |
| CN106632718B (en) * | 2016-12-28 | 2019-04-19 | 西安医学院 | The preparation method of bletilla polysaccharide |
| CN106632718A (en) * | 2016-12-28 | 2017-05-10 | 西安医学院 | Preparation method for rhizoma bletillae polysaccharide |
| CN106674370A (en) * | 2017-01-24 | 2017-05-17 | 贵州省武陵山药用植物白芨开发有限公司 | Bletilla striata gelatin and preparation method thereof |
| CN106893000A (en) * | 2017-03-16 | 2017-06-27 | 云南龙发制药股份有限公司 | A kind of Bletilla glucomannan extracting method |
| CN106832038A (en) * | 2017-03-30 | 2017-06-13 | 镇远县道益堂生物科技有限公司 | A kind of extracting method of bletilla polysaccharide |
| CN106883310A (en) * | 2017-04-17 | 2017-06-23 | 龙岩学院 | A kind of Deproteinated purification process of the very best beans polysaccharide decolouring |
| CN109364085A (en) * | 2018-10-29 | 2019-02-22 | 湖北中医药大学 | Bletilla striata oligosaccharides and combinations thereof is adjusting the application in glucose -lipid metabolism disorder |
| CN109364085B (en) * | 2018-10-29 | 2021-07-27 | 湖北中医药大学 | Application of bletilla striata oligosaccharide and composition thereof in regulation of glycolipid metabolic disorder |
| CN110075048A (en) * | 2019-06-03 | 2019-08-02 | 王金会 | A kind of Chinese herbal medicine cosmetic and preparation method thereof |
| CN110642960A (en) * | 2019-11-07 | 2020-01-03 | 皖西学院 | Double-aqueous-phase extraction method of bletilla striata glucomannan |
| CN110642960B (en) * | 2019-11-07 | 2021-12-10 | 皖西学院 | Double-aqueous-phase extraction method of bletilla striata glucomannan |
| CN111004335A (en) * | 2019-11-29 | 2020-04-14 | 湖南绿蔓生物科技股份有限公司 | Bletilla striata oligosaccharide extracted from rhizome of bletilla striata and preparation method thereof |
| CN113304088A (en) * | 2021-05-26 | 2021-08-27 | 上海辉文生物技术股份有限公司 | Preparation method of bletilla striata extract with repairing and whitening effects and bletilla striata extract |
| CN113354750A (en) * | 2021-05-31 | 2021-09-07 | 西安绿天生物技术有限公司 | Extraction method and application of bletilla striata polysaccharide with moisturizing effect |
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