CN104106726A - New probiotic, and probiotic fermentation feed prepared by using it - Google Patents
New probiotic, and probiotic fermentation feed prepared by using it Download PDFInfo
- Publication number
- CN104106726A CN104106726A CN201410060315.5A CN201410060315A CN104106726A CN 104106726 A CN104106726 A CN 104106726A CN 201410060315 A CN201410060315 A CN 201410060315A CN 104106726 A CN104106726 A CN 104106726A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus plantarum
- feed
- group
- parts
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a probiotic. The probiotic is Lactobacillus plantarum BS10 preserved in China Center for Type Culture Collection with the preservation number of CCTCC M2012487. The invention also discloses a use of the probiotic, and a feed prepared through fermenting by the probiotic. The Lactobacillus plantarum BS10 has lipid reducing, cholesterol reducing and bacterium inhibiting effects, is a probiotic, and can highly tolerate acidic environment and high bile salt environment. The feed prepared by using the Lactobacillus plantarum BS10 with the preservation number of CCTCC M2012487 can effectively promotes the growth of pigs and improve the disease resistance of the pigs, also can improve the quality of pork, and has a good application prospect.
Description
Technical field
The present invention relates to the new probio of a strain and with a fermented feed containing probiotics that plays preparation.
Background technology
Animal husbandry has in recent years obtained fast development, strengthens live pig resistance against diseases, improves growth performance and improves the important topic that meat is animal husbandry development.From the fifties in last century, in animal diets, add antibiotic and significantly promoted animal production, and the development of intensive animal husbandry has been made to major contribution.Yet As time goes on, in feed, add antibiotic harm and day by day manifest, and be subject to social extensive concern.2004, WTO, FAO (Food and Agriculture Organization of the United Nation) (FAO) and OIE (OIE) combined and hold symposium, and the antibiotic use of inhuman use and antibiotic drug resistance problem have been discussed.European Union completely forbids and in animal and fowl fodder, adds antibiotic from January, 2006.In the raisings such as live pig, must not add antibiotic has been internationally recognized food security standard at present.People start to seek one after another other substitute and substitute technology, unaffected to guarantee efficiency and the benefit of animal husbandry production.Fermented feed containing probiotics technology is the novel fodder technology with many advantages of recently growing up, feed contains more active probiotic thalline, various enzyme, metabolite at different levels, multivitamin, protein catabolite, active small peptide, amino acid, antibacterial substance, immune enhancer, somatomedin etc. after probiotics fermention, play and promote growth, maintaining the colony balance effect of animal intestinal, is a kind of environmental health type Feed Production Technology.
Probio is of a great variety, and Food and Drug Administration (FDA) and U.S. feed Gong Ding association (AAFCO) have announced and can be used as direct interpolation feed microbe and have more than 40 to plant.In the feed addictive kind catalogue of China Ministry of Agriculture issue in 2008, beneficial microbe has 16 kinds, comprise: bacillus licheniformis, bacillus subtilis, Bifidobacterium, enterococcus faecalis, VREF, lactoenterococcus, lactobacillus acidophilus, Lactobacillus casei, lactobacillus lactis, Lactobacillus plantarum, Pediococcus acidilactici, Pediococcus pentosaceus, candida utili, saccharomyces cerevisiae, Rhodopseudomonas palustris, lactobacillus bulgaricus.
Lactobacillus plantarum belongs to lactobacillus, and it is gram-positive bacteria, does not produce brood cell's nose circle straight-bar, single, in pairs or short chain shape, can produce D type, two kinds of lactic acid of L-type, can be 10 ℃ of growths, at 45 ℃, do not grow, optimum growth temperature scope is 30～35 ℃, amphimicrobian.In 4.5～9.5 growths of pH value, optimum pH is in 6.5 left and right.Lactobacillus plantarum is separated obtaining from saliva the earliest, and it is widely distributed, and is common in fermented food, comparatively common in the fermented food that the vegetable material of especially take is raw material.At present, find the Lactobacillus plantarum galactooligosaccharide of can degrading, can antagonism pathogenic bacteria, pathogenic very low, safety.Liu Jinping, " Lactobacillus plantarum A6(lactobacillus plantarum A6) biological characteristics and the research of applying in fermented feed ", Guangxi University 2004, the feed that discloses a kind of Lactobacillus plantarum A6 and fermentation preparation thereof, still, experimental result shows, compare with employing basal feed, adopt feed after the Lactobacillus plantarum A6 fermentation pig of feeding, pig average daily gain and reduce material anharmonic ratio without significant change, illustrates that it is difficult to effectively promote the growth of pig.
Summary of the invention
The invention provides a kind of new Lactobacillus plantarum probio, the fermented feed of preparing with aforementioned probio is also provided.
The probio that the present invention is new, it is the preserving number by the center preservation of Chinese Typical Representative culture collection: the plant BS10 of CCTCC M2012487 (Lactobacillus plantarum BS10).
Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10), on November 28th, 2012, be deposited in Chinese Typical Representative culture collection center (CCTCC), its address is: Wuhan, China Wuhan University, preserving number is CCTCC M2012487.
It is the preserving number by the center preservation of Chinese Typical Representative culture collection: the Lactobacillus plantarum BS10 of CCTCC M2012487 (Lactobacillus plantarum BS10).
The present invention also provides preserving number: the Lactobacillus plantarum BS10 of CCTCC M2012487 (Lactobacillus plantarum BS10) has the purposes in medicine, health products or the feed addictive of lipopenicillinase, norcholesterol, bacteriostasis in preparation.
In described medicine, health products or feed addictive, preserving number: the content of the Lactobacillus plantarum BS10 of CCTCC M2012487 is 2 * 10
6～2 * 10
10cFU/ml.Further preferably, in described medicine, health products or feed addictive, preserving number: the content of the Lactobacillus plantarum BS10 of CCTCC M2012487 is 2 * 10
6～2 * 10
8cFU/ml.
The preparation method of fermented feed containing probiotics of the present invention, step is as follows: in corn-soybean meal type feed, inoculation preserving number: the Lactobacillus plantarum BS10 of CCTCC M2012487 (Lactobacillus plantarum BS10), inoculum concentration is 0.5～1.5 * 10
6cFU/g, adjusting water content is 25～45%(w/w), under 15～35 ℃ of conditions, sealed fermenting 1～10d;
Described rice-bean pulp type feed is grouped into by the one-tenth of following weight proportion: 3～5 parts of 4～6 parts of auspicious pearl board compound premixs of 12～18 parts of rice brans of 10～16 parts of wheat bran of 60～67 parts of dregs of beans of corn.
Sealed fermenting: the fermentation that sealing is carried out in fermentation tank or fermentation bag.
Preferably, described rice-bean pulp type feed is grouped into by the one-tenth of following weight proportion: 4 parts of 5 parts of auspicious pearl board compound premixs of 15 parts of rice brans of 13 parts of wheat bran of 63 parts of dregs of beans of corn.
Auspicious pearl board compound premix, refers to pig compound premix or the large pig compound premix of auspicious pearl board 4% in auspicious pearl board 4% composite pre-mixed fodder for piglets, auspicious pearl board 4%.
Piglet: the pig that refers to body weight 20～40kg;
Middle pig: the pig that refers to body weight 40～60kg;
Large pig: refer to pig more than body weight 60kg.
Preferably, described inoculum concentration is 1 * 10
6cFU/g.
Preferably, described water content is 30～45%.Further preferably, described water content is 30～35%.
Preferably, the temperature of described fermentation is 30 ℃ or normal temperature.
Normal temperature: be also general temperature or room temperature, be generally 15～25 ℃.
Preferably, the time of described fermentation is 3～10 days.Further preferably, the time of described fermentation is 5～10 days.
The feed that the present invention also provides preceding method to prepare.
Preserving number of the present invention: the Lactobacillus plantarum BS10 of CCTCC M2012487 (Lactobacillus plantarum BS10), there is lipopenicillinase, norcholesterol and antibacterial effect, it is a kind of probio, sour environment and high cholate environment are had to good tolerance, can be used for medicine, health products or feed addictive that preparation has lipopenicillinase, norcholesterol, bacteriostasis.Adopt preserving number of the present invention: fermented feed prepared by the Lactobacillus plantarum BS10 of CCTCC M2012487, can effectively promote the growth of pig, improve the premunition of pig, can also improve meat quality, its preparation method is simple, with low cost, and application prospect is good.
Obviously, according to foregoing of the present invention, according to ordinary skill knowledge and the customary means of this area, not departing under the above-mentioned basic fundamental thought of the present invention prerequisite, can also make modification, replacement or the change of other various ways.
The specific embodiment of form, is described in further detail foregoing of the present invention again by the following examples.But this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following example.All technology realizing based on foregoing of the present invention all belong to scope of the present invention.
Accompanying drawing explanation
The removing cholesterol rate result figure of Fig. 1 Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10)
The specific embodiment
Experiment material:
| Name of product | Authentication code | Sale enterprise title |
| Auspicious pearl board 4% composite pre-mixed fodder for piglets | Pre-word (2008) 302002 is raised in river | Sichuan Zhengdong Farming Group Co., Ltd. |
| Pig compound premix in auspicious pearl board 4% | Pre-word (2008) 302003 is raised in river | Sichuan Zhengdong Farming Group Co., Ltd. |
| The large pig compound premix of auspicious pearl board 4% | Pre-word (2008) 302004 is raised in river | Sichuan Zhengdong Farming Group Co., Ltd. |
All the other raw materials are commercially available product.
The identification mark of embodiment 1 Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10)
1, authentication method
By the separated bacterial strain of the present invention obtaining the biased sample from chitling road and fermented feed, carry out following morphology and molecular biology identification.
(1) identification by morphological characters
Utilize microscope and visually observe cell and solid plate on colonial morphology.
(2) molecular biology identification
By the total DNA of bacterial strain of the present invention after pcr amplification l6S rDNA gene, serve the order-checking of Hai Sheng work Co., Ltd, application Chromas2 software is edited measuring sequence, in GenBank (https://www.ncbi.nlm.nih.gov/BLAST/) database, compares.
2, qualification result
(1) morphological feature
Thalline is rod-short, atrichia, and without pod membrane, Gram's staining is positive.
(2) 16S rDNA sequence and analysis
The 16S rDNA sequence length that PCR has obtained bacterial strain of the present invention is 835bp, and sequence is as follows:
GCGATTCCTATACTGCAGTCGAACGAACTCTGGTATTGATTGGTGCTTGCATCATGATTTACATTTGAGTGAGTGGCGAACTGGTGAGTAACACGTGGGAAACCTGCCCAGAAGCGGGGGATAACACCTGGAAACAGATGCTAATACCGCATAACAACTTGGACCGCATGGTCCGAGCTTGAAAGATGGCTTCGGCTATCACTTTTGGATGGTCCCGCGGCGTATTAGCTAGATGGTGGGGTAACGGCTCACCATGGCAATGATACGTAGCCGACCTGAGAGGGTAATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGATGGAGCAACGCCGCGTGAGTGAAGAAGGGTTTCGGCTCGTAAAACTCTGTTGTTAAAGAAGAACATATCTGAGAGTAACTGTTCAGGTATTGACGGTATTTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTTTTAAGTCTGATGTGAAAGCCTTCGGCTCAACCGAAGAAGTGCATCGGAAACTGGGAAACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGTAACTGACGCTGAGGCTCGAAAGTATGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCATACCGTAAACGATGAATGCTAAGTGTTGGAGGGTTTCCGCCCTTCCG
Utilize BLAST software that the DNA sequence dna of including in this sequence and GenBank is compared, and build chadogram to relevant bacterial classification.Result demonstration, the similitude of invention isolated strains and Lactobacillus plantarum reaches 98%, shows that it is Lactobacillus plantarum.
Combining form feature and molecular biology identification result, isolated strains of the present invention is accredited as to Lactobacillus plantarum (Lactobacillus plantarum), called after Lactobacillus plantarum BS10 (Lactobacillus plantarum BS10), and being deposited in Chinese Typical Representative culture collection center (CCTCC) on November 28th, 2012, preserving number is CCTCC M2012487.
The Characteristics Detection of embodiment 2 Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10)
One, acidproof bile tolerance characteristic
1, experimental technique
Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) the bacterium liquid in 3 generations of activation, by the 2% MRS fluid nutrient medium that is inoculated in respectively pH3.0 and pH6.4, is cultivated to the growing state of observing bacterium after 12 hours for 37 ℃, and record.By the 2% MRS fluid nutrient medium being inoculated in containing 0.3g/100ml bovine bile, cultivate after 12 hours for 37 ℃ each bacterial strain is carried out to count plate simultaneously.Test repeats to average for 3 times.
MRS fluid nutrient medium: peptone 10g, beef extract 5g, yeast extract 4g, glucose 20g, Tween-80 1ml, dipotassium hydrogen phosphate 2g, sodium acetate 5g, citric acid tri-amonia 0.2g, magnesium sulfate (MgSO
47H
2o) 0.2g, manganese sulfate (MnSO
44H
2o) 0.05g, water 1000ml.
2, experimental result
Testing result is as shown in table 1:
The tolerance of table 1 pair PHp.0 sour environment and 0.3g/100ml cholate environment
Note: " +++ " represents that growth is good; " ++ " represents well-grown; "+" represents that growth is general; " +/-" represents slightly growth; "-" represents that growth is bad.
Experimental result shows, Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) well-grown under sour environment and high cholate environment, shows that it has the tolerance of good sour environment and high cholate environment.
Two, norcholesterol characteristic
1, experimental technique
By activation Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) the bacterium liquid in 3 generations, by 2%, be inoculated in MRS-CHOL culture medium (MRS-CHOL culture medium: containing the MRS fluid nutrient medium of cholesterol 100mg/L and 0.3g/100mL bovine bile), cultivate after 24 hours for 37 ℃ and measure bacterium liquid supernatant with o-phthalaldehyde method, cleaning solution, the mass fraction of cholesterol in bacterial cell disruption liquid, using the MRS-CHOL culture medium of inoculating lactic acid bacterium not as blank, and according to the removal efficiency of cholesterol in the mass fraction calculating culture medium of cholesterol in supernatant, test repeats to average for 3 times.
Supernatant: bacterial strain is cultivated the liquid phase part after the centrifugal 20min of 5000r/min after 24 hours in MRS-CHOL culture medium;
Cleaning solution: add 10ml(0.1mol/L, pH7.0 inclining in the somatic cells of supernatant) PBS buffer saline, washs the liquid of the centrifugal 20min gained of 5000r/min after 2 times;
Broken liquid: incline and thalline cleaning solution, then add 5.0ml PBS buffer solution, put in ice bath, ultrasonic cell disruptor power is under 600W after broken 10min, the centrifugal 10min gained of 4000r/min liquid.
O-phthalaldehyde method detects the amount of cholesterol in supernatant, cleaning solution, bacterial cell disruption liquid:
1. get respectively 1ml suspension and add 1mlKOH(mass fraction 33%) and 2ml absolute ethyl alcohol vibration 1min fully mix rear 37 ℃ of heating 15min.2. after cooling, add 2ml distilled water and 3ml n-hexane vibration 1min fully to mix, stratification.3. get 1ml n-hexane layer and move into glass tube, glass tube is put into 60 ℃ of water-baths dry.4. after drying, residue is dissolved in the O-phthalic aldehyde reagent of 2ml immediately, after fully mixing, adds 0.5ml concentrated sulfuric acid vibration 1min to mix.5. after standing 10min, under 550nm, measure OD value.
2, experimental result
Experimental result is as shown in Figure 1: after adopting Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) to process, in culture medium, the content of cholesterol only 44.99%, illustrate that Lactobacillus plantarum BS10 of the present invention can effectively reduce cholesterol level, can reduce the incidence probability of angiocardiopathy.
Three, bacteriostasis
1, experimental technique
By pathogenic bacteria: Escherichia coli CVCC2060, salmonella 339, staphylococcus aureus and Pseudomonas aeruginosa activated for 3 generations, and overnight culture is diluted to 10
5cFU/ml, gets respectively 0.1ml coating nutrient agar panel, and Oxford cup is evenly placed on flat board; Add wherein 200ul to activate Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) the bacterium liquid after 3 generations; Cultivate 24h for 37 ℃, measure antibacterial circle diameter.
Repeating to test 3 times averages.
2, experimental result
Experimental result is as shown in table 2:
Table 2 bacterium result (antibacterial circle diameter)
| Salmonella (mm) | Staphylococcus aureus (mm) | Escherichia coli (mm) | Pseudomonas aeruginosa (mm) |
| 10.83 | 10.80 | 10.90 | 17.00 |
As shown in table 2, Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) all has stronger inhibitory action to common pathogen salmonella, staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, wherein, the strongest to the inhibitory action of Pseudomonas aeruginosa.
Four, the effect of lipopenicillinase and regulating intestinal canal flora in body
1, test method
(1) preparation of high lipid food
High lipid food: normal diet (every 100g normal diet comprises 50g corn, 20g wheat bran, 15g soya bean, 10g flour and 5g fish meal) 80%, cholesterol 0.5%, lard 6.3%, yolk powder 13%, cholate 0.2%.Mix and with granulator, granulate afterwards, air-dry as for aeration-drying place.
MRS nutrient solution: peptone 10g, beef extract 5g, yeast extract 4g, glucose 20g, Tween-80 1ml, dipotassium hydrogen phosphate 2g, sodium acetate 5g, citric acid tri-amonia 0.2g, magnesium sulfate (MgSO
47H
2o) 0.2g, manganese sulfate (MnSO
44H
2o) 0.05g, water 1000ml, pH6.2 ± 0.2.
(2) preparation of gavage bacterium liquid
Get Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10), activation, the bacterium liquid by activation after 3 generations increases bacterium cultivation by 2% inoculum concentration with MRS nutrient solution, cultivates after 36 hours, by point sample method, carries out count plate.The PBS buffer solution for MRS nutrient solution (phosphate buffer normal saline PH7.4) increasing after bacterium is concentrated or is diluted to high, medium and low concentration bacterium liquid, be respectively 2 * 10
10, 2 * 10
8, 2 * 10
6cFU/ml, divides bacterium liquid concentrated or that diluted to install in 15ml centrifuge tube and be stored in-20 ℃ of refrigerators standbyly, prepares weekly bacterium liquid one time.Bacterium liquid is placed in 25 ℃ of warm water and uses after thawing.
(3) animal grouping and detection
48 SD rats (male, 130g left and right) are divided into 5 groups, every group of 6 rats at random: basic control group, obese model group, BS10 I group (2 * 10
6cBS10U/ml), BS10 II group (2 * 10
8cBS10U/ml), BS10 III group (2 * 10
10cBS10U/ml).
Every cage is raised 6 rats, keeps 23 ℃ of constant temperature, and the first seven sky basal feed of feeding, conforms it.After seven days, basic control group fed normal diet gavage PBS buffer solution; Obese model group and test group group feed high lipid food respectively gavage PBS buffer solution and variable concentrations bacterium liquid, dosage is 0.5ml/100gbwd.Ad lib drinking-water, claims weekly body weight one time.
Test after 8 weeks, fasting 12 hours, can't help water.Use etherization rat, eyeball is got blood, and after static 30min, centrifugal 10 minutes of 4000r/min, gets serum, measures respectively TG, TC content; Win liver organization, stomach fat, perirenal fat pad and epididymal adipose tissues pad and weigh, calculate body index (histoorgan weight/body weight); Cecal content mixes in rear packing and centrifuge tube ,-70 ℃ of preservations.
2, experimental result
(1), the impact on adipopexis
Experimental result is as shown in table 3:
The impact of table 3 on fat deposition index of correlation
Compare with high fat group,
*p<0.01,
*p<0.05.
As shown in table 3:
Compare with Normal group, the body weight of high fat group, liver body index, abdomen fat body index, perirenal fat pad index, epididymal adipose tissues pad index, TG, TC all obviously raise (p<0.01 or p<0.05), and hyperlipidemia model modeling success is described.
Compare with high fat group, BS10 I of the present invention, BS10 II and BS10 III all can effectively reduce body weight, liver body index, abdomen fat body index, perirenal fat pad index, epididymal adipose tissues pad index, TG, TC and all significantly reduce, wherein, all there is significant difference (P<0.05 or P<0.01) in the indices of BS10 I group and BS10 II and high fat group, abdomen fat body index, TG, TC and the high fat group of BS10 III group all exist significant difference (P<0.05 or P<0.01).
Experimental result explanation, Lactobacillus plantarum of the present invention (Lactobacillus plantarum BS10) has obvious effect for reducing fat, and wherein, cell concentration is 2 * 10
6～2 * 10
8during CBS10U/ml, effect is more excellent, and concentration is 2 * 10
6during CBS10U/ml, effect is optimum.
(2) impact on alimentary canal bacterial number
The impact of table 4 on rat cecal content bacterial number
Compare with high fat group, * represents significant difference P < 0.05, and * * represents that difference is extremely remarkable, P < 0.01.
As shown in table 4, compare with control group, high lipid food can cause the reduction of other bacteriums except aerobic bacteria in enteron aisle, and lactic acid bacteria and Bifidobacterium reduction remarkable (P<0.05), in enteron aisle, Escherichia coli and enterococcus ratio raise, and aerobic bacteria/anaerobic bacteria relative scale raises.
After gavage Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10), compare with high fat group, in enteron aisle, Escherichia coli, enterococcus and aerobic total bacteria count change not obvious, but Bacillus acidi lactici, Bifidobacterium and anaerobic bacteria total bacteria count raise, and be changed significantly (P<0.05 or the P<0.01) of BS10 I group and BS10 II.
Experimental result explanation, Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) can improve probio quantity, reduces harmful bacteria ratio, and wherein, cell concentration is 2 * 10
6～2 * 10
8during CFU/ml, effect is more excellent, and concentration is 2 * 10
6during CFU/ml, effect is optimum.
To sum up, Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) has lipopenicillinase, norcholesterol and antibacterial effect, and wherein, cell concentration is 2 * 10
6～2 * 10
10during CFU/ml, effect is more excellent, and concentration is 2 * 10
6during CFU/ml, effect is optimum, and sour environment and high cholate environment are had to good tolerance, can be used for medicine, health products or feed addictive that preparation has lipopenicillinase, norcholesterol, bacteriostasis.
Embodiment 3 adopts Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) to prepare feed
1, experiment material
Fermentation base-material: corn-soybean meal type feed.
Corn-soybean meal type feed comprises 3 kinds:
Piglet corn-soybean meal type feed (100g): corn 63g, dregs of beans 13g, wheat bran 15g, rice bran 5g, auspicious pearl board 4% composite pre-mixed fodder for piglets 4g.
Middle pig corn-soybean meal type feed (100g): pig compound premix 4g in corn 63g, dregs of beans 13g, wheat bran 15g, rice bran 5g, auspicious pearl board 4%.
Large pig corn-soybean meal type feed (100g): corn 63g, dregs of beans 13g, wheat bran 15g, rice bran 5g, the large pig compound premix 4g of auspicious pearl board 4%.
Fermented bacterium: Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10), viable count is 1 * 10
9cFU/ml.
2, experimental technique
In corn-soybean meal type feed, add Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10), addition is 1 * 10
6cFU/g feed, regulates moisture with distilled water, and A, B, C, D and E totally 5 processing are established in test altogether, and moisture content is respectively 25%, 30%, 35%, 40% and 45%, mixes rear with sealed fermenting bag packing fermentation, 30 ℃ of standing cultivations of incubator.Each processes 3 repetitions.
Testing index and method:
1. sample pH value pH-value determination pH is 80% with distilled water adjusting sample moisture content, after stirring, directly measures.
2. lactic acid content measure to adopt lactic acid content in xenol colorimetric method for determining fermented feed (with reference to Luo Jian etc., " the assay method comparative analysis of lactic acid content in fermentative feedstuff of microbe ", feed review, 05 disclosed method of phase was measured in 2012).
3. the dry rate of recovery (DMR) is weighed after measuring fermented feed preparation immediately, and after this, in fermentation the 1st, 3,5,7,10d sampling, 105 ℃ of oven dry are weighed.Calculate the fermented feed dry rate of recovery (DMR).
4. Bacillus acidi lactici quantitative measurement accurately takes fermented feed 10.0g, with 10 times, physiological saline, increases progressively and is diluted to 10-7, gets suitable dilution sample drop kind in MRS culture medium, and 37 ℃ of anaerobism are cultivated 24-36h, according to Bacillus acidi lactici quantity in clump count calculation sample.
3, experimental result
(1) impact that, moisture changes fermented feed pH value
Experimental result is as shown in table 5:
The impact that table 5 moisture changes fermented feed pH value
Note: colleague's data shoulder mark same letter represents difference not remarkable (P>0.05), different lowercase alphabets show significant difference (P<0.05), and different capitalizations represent difference extremely significantly (P<0.01).Following table is same.
As known from Table 5, at each time point of fermentation, A group and B group pH value are all significantly higher than all the other each groups (P < 0.01).The 1d that ferments, each is organized fermented feed pH value and is all reduced to rapidly 5.0 left and right, and moisture is higher, reduces more obvious; At 3d, pH value further reduces, and difference all extremely significantly (P < 0.01) between each group.To 5d, pH value fall off rate obviously slows down, and D group pH value is minimum, is 4.52, extremely significantly lower than all the other each groups (P < 0.01).7d and 10d, it is basicly stable in 4.5 left and right that each organizes pH value, and D group is significantly lower than C group and E group (P < 0.05).
(2) impact that moisture changes fermented feed lactic acid content
Experimental result is as shown in table 6:
The impact (m mol/g) that table 6 moisture changes fermented feed lactic acid content
As shown in Table 6, along with the prolongation of fermentation time, each is organized lactic acid content and first raises, and peaking after 5d slowly reduces afterwards.At 1d and 3d, C group and D group the lactic acid content all utmost point are significantly higher than A group and B group (P < 0.01), C group and D group and E group and E organizes and A group and B group between difference all not remarkable (P > 0.05).5d～10d, C group and D group lactic acid content are significantly higher than A group (P < 0.05), but organize difference not remarkable (P > 0.05) with B group and E.
Therefore, preferably fermentation time is 3～10 days, more preferably 5～7 days; Preferred water is divided into 30～45%, and more preferably 35%.
(3) impact of moisture on fermented feed DMR changes of contents
Experimental result is as shown in table 7:
The impact of table 7 moisture on fermented feed DMR
As shown in Table 7, along with the prolongation of fermentation time, each is organized DMR and all reduces gradually.And reducing amount and moisture are proportionate.At 1d, E group DMR significantly organizes and B group (P < 0.05) lower than A, and organizes difference not remarkable (P > 0.05) with C group and D.At 3d and 5d, DMR is significantly lower than A group (P < 0.05) for B group, C, D and E group are all extremely significantly lower than A group (P < 0.01), and D, E organize significantly lower than C, D group (P < 0.05).To 7d, A group is organized DMR difference not remarkable (P > 0.05) with B, and C, D and E group are all extremely significantly lower than A group (P < 0.01).D and E group are significantly lower than C group (P < 0.05), extremely significantly lower than B group (P < 0.01).To 10d, difference all not significantly (P > 0.05) between two adjacent groups, non-conterminous two group differences are extremely significantly (P < 0.01) all.
(4) impact of moisture on fermented feed Bacillus acidi lactici number change
Experimental result is as shown in table 8:
As shown in Table 8, each organizes Bacillus acidi lactici quantity 3d before fermentation increases gradually, starts afterwards slow minimizing.At 1d, C and the D group Bacillus acidi lactici quantity utmost point are significantly higher than A, B and E group (P < 0.01).At 3d, the D group utmost point is significantly higher than A, B and E group (P < 0.01), and C group is significantly higher than B group and E group (P < 0.05).During 5d, B group and the C group utmost point are significantly higher than A group (P < 0.01), and D group and the E group utmost point are significantly higher than A, B and C group (P < 0.01).During to 7d, B, C and the D group utmost point are significantly higher than A group and E group (P < 0.01), E group is significantly higher than A group (P < 0.05), and B, C and D group difference be remarkable (P > 0.05) not.To 10d, each is organized Bacillus acidi lactici quantity and all drops to 10
8the CFU/g order of magnitude.B, C, D and E organize all utmost points and are significantly higher than A group (P < 0.01), and B, C and the D group utmost point are significantly higher than E group, and B, C and D group difference be remarkable (P > 0.05) not.
Therefore, preferred water is divided into 30～45%, and more preferably 30～35%.
To sum up, while adopting Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) fermentation to prepare fermented feed, the water content of raw material can be 25～45%, is preferably 30～45%, more preferably 30～35%; The time of fermentation can be 1～10 day, is preferably 3～10 days, more preferably 5～10 days.
Below the mode of example illustrates beneficial effect of the present invention by experiment:
Experimental example 1 adopts Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) to prepare the impact of fermented feed on pig growth
One, experimental technique
1, experimental design and test daily ration
2 processing are established in test altogether, and each processes 4 repetitions, and each repeats 3 pigs.
Processing 1 is control group, the corn-soybean meal of feeding type feed (comprise piglet corn-soybean meal type feed, middle pig corn-soybean meal type feed and large pig corn-soybean meal type feed, formula refers to embodiment 3);
Processing 2 is BS10 group, the 0.1% Lactobacillus plantarum BS10 fermented feed of feeding, the preparation method of feed: (comprise piglet corn-soybean meal type feed, middle pig corn-soybean meal type feed and large pig corn-soybean meal type feed at corn-soybean meal type feed, formula refers to embodiment 3) the middle Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) that adds, addition is 1 * 10
6cFU/g feed, it is 35% that the distilled water of take regulates moisture to moisture content, mixes the rear sealed fermenting bag packing fermentation of using, normal temperature fermentation 7 days, obtains respectively piglet of the present invention, middle pig and hog grower feed.
2, feeding and management
Test is carried out on Xin Bo pig farm, Yanjiang District, Ziyang City, Sichuan Province.Expection 7d, is just trying phase 136d, from year July in March, 2012 to 2012.Test is carried out according to the conventional feeding and management in pig farm, and every day, 8:00,14:00,20:00 fed, and freely drank water.Experiment pig, the piglet of average weight 20kg.
Piglet stage (body weight 20～40kg) the 2 kinds of pig starter feeds of feeding respectively, the pig feed in 2 kinds of feeding respectively of middle pig stage (body weight 40～60kg), the 2 kinds of hog grower feeds of feeding respectively of large pig stage (more than body weight 60kg), the scale of feeding of piglet is 1.10kg/ head/sky, the scale of feeding of middle pig is 1.9kg/ head/sky, and the scale of feeding of large pig is 2.25kg/ head/sky.
3, testing index
(1) growth performance index
Test is divided into 2 stages: growth period 20～50kg and the stage of fattening 50～100kg.When average weight reaches respectively 50kg and 100kg left and right, weigh on an empty stomach, calculate average daily ingestion amount (ADBS10I), daily gain (ADG) and the feed weightening finish of growth period, the stage of fattening and full phase than (BS10/G).
Computational methods are as follows: average daily gain (ADG)=(end weight-initial weight)/test number of days; Average daily ingestion amount (ADBS10I)=(remaining material amount in experimental period in feed quantity-experimental period)/test number of days; Material anharmonic ratio (BS10/G)=ADG/ADBS10I.
(2) meat index
After feeding experiment finishes, every group is selected each 4 of the pigs that body weight approaches average level to butcher, detects meat index.With Denmark, produce yellowish pink analyzer and pH analyzer and measure yellowish pink and pH value respectively at butchering rear 45min and 24h.Drip loss and shearing force assay method are with reference to People's Republic of China's agricultural industry criteria NY/T821-2004 < < pig muscle quality determination technical specification > >.
(3) Biochemical Indices In Serum
Adopt fully automatic blood Biochemical Analyzer to measure the content of serum glucose (GLU), triglycerides (TG), T-CHOL (TC), HDL (HDL-C), low-density lipoprotein (LDL-C), serum urinary nitrogen (BUN), total serum protein (TP), seralbumin (ALB) and serum globulins (GLB).
(4) Serum antioxidant indices
SOD, CAT, GSH-Px and MDA measure kit and all purchased from Nanjing, build up biological study institute, by kit explanation, operate.
Two, result of the test
(1) impact of lactobacillus fermentation feed on pig growth performance
The impact of table 9 fermented feed on pig growth performance
Note: colleague's data shoulder mark same letter represents difference not remarkable (P>0.05), different lowercase alphabets show significant difference (P<0.05), and different capitalizations represent difference extremely significantly (P<0.01).Following table is same.
As shown in Table 9:
In growth period, each organizes ADG, ADFI and F/G difference all not significantly (P > 0.05).
At fattening period, compare with control group, the ADG(average daily gain of BS10 group of the present invention) improved 7.26%, F/G(expects anharmonic ratio) reduced by 13%, difference all reaches the level of signifiance (P < 0.01 and P < 0.05), and ADFI is without significant change (P > 0.05).
In whole experimental period, compare the ADG(average daily gain of BS10 of the present invention group with control group) improved 9.00%, F/G(material anharmonic ratio) having reduced by 9.30%, difference all reaches the utmost point level of signifiance (P < 0.01).
Experimental result explanation, the feed that adopts Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) fermentation to prepare can improve the average daily gain of pig effectively, reduces material anharmonic ratio, promotes the growth of pig, reduces production costs.
(2) impact of lactobacillus fermentation feed on Meat performance
The impact of table 10 fermented feed on meat quality
As shown in Table 10:
The pH of two groups and drip loss are all in critical field.
Compare with control group, the brightness of BS10 of the present invention group is higher, and shearing force is lower, significant difference (P < 0.05), and red degree, yellow degree are suitable with control group.
Experimental result explanation, the feed that adopts Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) to prepare can improve pork brightness, reduces its shearing force, improves pork tenderness.
(3) impact of lactobacillus fermentation feed on pig Biochemical Indices In Serum
The impact of table 11 fermented feed on pig Biochemical Indices In Serum
As can be seen from Table 11:
Compare with negative control group, glucose, HDL-C and the triglycerides of BS10 group of the present invention reduce.
, after testing, compare with negative control, the pig intramuscular fat content of BS10 group of the present invention has reduced by 2.5% meanwhile, and content of polyunsaturated fatty acid has reduced by 2.1%.
Experimental result explanation, the feed of Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) fermentation preparation, can effectively reduce the fat ratio of pork, improves the market competitiveness of pork.
(4) impact of lactobacillus fermentation feed on pig serum antioxygenic property
The impact of table 12 fermented feed on pig serum antioxygenic property
| Project | Control group | BS10 group (Bacillus acidi lactici B10) |
| SOD（U·mL -1） | 105.20±9.19 a | 121.36±6.41 b |
| MDA（nmol·mL -1） | 7.02±0.44 Aa | 4.50±0.22 Bb |
| GSH-Px（U·mL -1） | 253.22±21.38 a | 288.39±9.47 ab |
As shown in Table 12:
Compare with negative control group, the SOD value of BS10 group of the present invention and GSH-Px value obviously improve (P < 0.05), and MDA value obviously reduces (P < 0.05).
, after testing, compare with negative control, live pig reduces the intestines problems 80% such as diarrhoea meanwhile.
Experimental result explanation, Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) can effectively improve the premunition of pig.
BS10 group of the present invention is compared with control group, and the daily gain of pig has improved 9.00%, and feedstuff-meat ratio has reduced by 9.30%, saves feed cost 8%, and live pig reduces the intestines problems 80% such as diarrhoea, and every pig is saved 16 yuan of medication expenditures; Pork brightness is significantly higher than control group, drip loss has reduced by 12.2% than control group, tender degree has improved 13.5%, and pig intramuscular fat content has reduced by 2.5%, and content of polyunsaturated fatty acid has reduced by 2.1%, meat quality significantly improves, during market sale, the pork price of control group is 16 yuan/kg, and the price of BS10 group of the present invention can reach 18-20 unit/kg, improve direct economic benefit 12-25%, obtained coml success.
Adopt Lactobacillus plantarum BS10 of the present invention (Lactobacillus plantarum BS10) to prepare feed, can effectively improve the average daily gain of pig, reduce material anharmonic ratio, increase pork brightness, reduce pork shearing force, improve pork tenderness, improve pig growth performance and meat quality, can also effectively improve the premunition of pig.
To sum up, preserving number of the present invention: the Lactobacillus plantarum BS10 of CCTCC M2012487 (Lactobacillus plantarum BS10), there is lipopenicillinase, norcholesterol and antibacterial effect, sour environment and high cholate environment are had to good tolerance, can be used for medicine, health products or feed addictive that preparation has lipopenicillinase, norcholesterol, bacteriostasis.Adopt preserving number of the present invention: feed prepared by the Lactobacillus plantarum BS10 of CCTCC M2012487, can promote pig growth, improve meat quality, can also effectively improve the premunition of pig, and preparation method is simple, with low cost, application prospect is good.
Claims (10)
1. a probio, is characterized in that: it is the preserving number by the center preservation of Chinese Typical Representative culture collection: the Lactobacillus plantarum BS10 of CCTCC M2012487 (Lactobacillus plantarum BS10).
2. preserving number: the Lactobacillus plantarum BS10 of CCTCC M2012487 (Lactobacillus plantarum BS10) has the purposes in medicine, health products or the feed addictive of lipopenicillinase, norcholesterol, bacteriostasis in preparation.
3. the purposes in medicine according to claim 2, health products or feed addictive, is characterized in that: in described medicine, health products or feed addictive, and preserving number: the content of the Lactobacillus plantarum BS10 of CCTCC M2012487 is 2 * 10
6～2 * 10
10cFU/ml;
Preferably, in described medicine, health products or feed addictive, preserving number: the content of the Lactobacillus plantarum BS10 of CCTCC M2012487 is 2 * 10
6～2 * 10
8cFU/ml.
4. the preparation method of a fermented feed containing probiotics, it is characterized in that: step is as follows: in corn-soybean meal type feed, inoculation preserving number: the Lactobacillus plantarum BS10 of CCTCC M2012487 (Lactobacillus plantarum BS10), inoculum concentration is 0.5～1.5 * 10
6cFU/g, adjusting water content is 25～45%(w/w), under 15～35 ℃ of conditions, sealed fermenting 1～10d;
Described corn-soybean meal type feed is grouped into by the one-tenth of following weight proportion: 60～68 parts of corns, 10～16 parts of dregs of beans, 12～18 parts, wheat bran, 4～6 parts, rice bran, 3～5 parts of auspicious pearl board compound premixs.
5. preparation method according to claim 4, is characterized in that: described corn-soybean meal type feed is grouped into by the one-tenth of following weight proportion: 64 parts of corns, 13 parts of dregs of beans, 15 parts, wheat bran, 5 parts, rice bran, 4 parts of auspicious pearl board compound premixs.
6. preparation method according to claim 4, is characterized in that: described inoculum concentration is 1 * 10
6cFU/g.
7. preparation method according to claim 4, is characterized in that: described water content is 30～45%(w/w); Preferably, described water content is 30～35%(w/w).
8. preparation method according to claim 4, is characterized in that: the temperature of described fermentation is 30 ℃ or normal temperature.
9. preparation method according to claim 4, is characterized in that: the time of described fermentation is 3～10 days; Preferably, the time of described fermentation is 5～7 days.
10. the fermented feed that described in claim 4～9 any one, method prepares.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410060315.5A CN104106726A (en) | 2014-02-21 | 2014-02-21 | New probiotic, and probiotic fermentation feed prepared by using it |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410060315.5A CN104106726A (en) | 2014-02-21 | 2014-02-21 | New probiotic, and probiotic fermentation feed prepared by using it |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104106726A true CN104106726A (en) | 2014-10-22 |
Family
ID=51703871
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410060315.5A Pending CN104106726A (en) | 2014-02-21 | 2014-02-21 | New probiotic, and probiotic fermentation feed prepared by using it |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104106726A (en) |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105112334A (en) * | 2015-04-27 | 2015-12-02 | 南昌大学 | Microecological preparation comprising lactobacillus plantarum |
| CN105132322A (en) * | 2015-09-02 | 2015-12-09 | 广州格拉姆生物科技有限公司 | Lactobacillus plantarum and application thereof |
| CN105505813A (en) * | 2015-11-12 | 2016-04-20 | 南昌大学 | Strongly-bacteriostatic lactobacillus plantarum |
| CN105567601A (en) * | 2016-01-29 | 2016-05-11 | 江苏绿科生物技术有限公司 | Lactobacillus plantarum and application thereof |
| CN107312726A (en) * | 2017-05-27 | 2017-11-03 | 武汉科缘生物发展有限责任公司 | One lactobacillus plantarum ZN 3 and application |
| CN107889935A (en) * | 2017-12-04 | 2018-04-10 | 大连百安泰生物科技有限公司 | A kind of semi-moist shape probiotic composition and preparation method and application |
| CN109007280A (en) * | 2018-08-20 | 2018-12-18 | 广州海诚生物科技有限公司 | A kind of biofermentation type function bighead feed |
| CN109007445A (en) * | 2018-08-20 | 2018-12-18 | 广州海诚生物科技有限公司 | A kind of biofermentation type function dace feed |
| CN109965083A (en) * | 2019-04-17 | 2019-07-05 | 佛山播恩生物科技有限公司 | A kind of depth fermented feed production method containing two kinds of high-content organic acids |
| CN111269850A (en) * | 2020-01-20 | 2020-06-12 | 吉林农业大学 | Pediococcus pentosaceus PP04 with high adhesion capability and blood fat reducing effect |
| CN111436526A (en) * | 2020-04-01 | 2020-07-24 | 浙江大学 | Preparation method and application of fermented rice bran meal with bacterium enzyme for improving growth performance of fattening pigs |
| CN112899200A (en) * | 2021-03-04 | 2021-06-04 | 江西格力特农牧发展有限公司 | Constant-temperature fermented liquid lactic acid bacteria and preparation method and application thereof |
| CN113897312A (en) * | 2021-10-09 | 2022-01-07 | 中国科学院成都生物研究所 | Preparation and application of animal feeding microbial inoculum |
| CN115152893A (en) * | 2022-07-04 | 2022-10-11 | 广西农业职业技术大学 | Compound probiotic composition for Qingyuan spicy-feather chicks and preparation method thereof |
| CN117016691A (en) * | 2023-08-09 | 2023-11-10 | 宁夏三十六度生物科技有限公司 | Probiotic feed additive for improving pork quality |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101558838A (en) * | 2009-05-03 | 2009-10-21 | 陈志望 | Pannage and production process thereof |
| CN102031229B (en) * | 2009-09-24 | 2012-05-30 | 家医国际生化股份有限公司 | Lactobacillus plantarum with gastrointestinal adsorption capacity and cholesterol removal capacity |
| CN102548566A (en) * | 2009-10-09 | 2012-07-04 | 抗体抗生素股份有限公司 | Lactobacillus plantarum strains as hypocholesterolemic agents |
| CN103205376A (en) * | 2013-03-15 | 2013-07-17 | 厦门和美科盛生物技术有限公司 | Lactobacillus plantarum applicable to fermented feed and application of lactobacillus plantarum |
-
2014
- 2014-02-21 CN CN201410060315.5A patent/CN104106726A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101558838A (en) * | 2009-05-03 | 2009-10-21 | 陈志望 | Pannage and production process thereof |
| CN102031229B (en) * | 2009-09-24 | 2012-05-30 | 家医国际生化股份有限公司 | Lactobacillus plantarum with gastrointestinal adsorption capacity and cholesterol removal capacity |
| CN102548566A (en) * | 2009-10-09 | 2012-07-04 | 抗体抗生素股份有限公司 | Lactobacillus plantarum strains as hypocholesterolemic agents |
| CN103205376A (en) * | 2013-03-15 | 2013-07-17 | 厦门和美科盛生物技术有限公司 | Lactobacillus plantarum applicable to fermented feed and application of lactobacillus plantarum |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105112334A (en) * | 2015-04-27 | 2015-12-02 | 南昌大学 | Microecological preparation comprising lactobacillus plantarum |
| CN105112334B (en) * | 2015-04-27 | 2018-08-17 | 南昌大学 | Probiotics containing lactobacillus plantarum |
| CN105132322A (en) * | 2015-09-02 | 2015-12-09 | 广州格拉姆生物科技有限公司 | Lactobacillus plantarum and application thereof |
| CN105132322B (en) * | 2015-09-02 | 2016-08-31 | 广州格拉姆生物科技有限公司 | One lactobacillus plantarum and application thereof |
| CN105505813A (en) * | 2015-11-12 | 2016-04-20 | 南昌大学 | Strongly-bacteriostatic lactobacillus plantarum |
| CN105567601A (en) * | 2016-01-29 | 2016-05-11 | 江苏绿科生物技术有限公司 | Lactobacillus plantarum and application thereof |
| CN107312726A (en) * | 2017-05-27 | 2017-11-03 | 武汉科缘生物发展有限责任公司 | One lactobacillus plantarum ZN 3 and application |
| CN107889935A (en) * | 2017-12-04 | 2018-04-10 | 大连百安泰生物科技有限公司 | A kind of semi-moist shape probiotic composition and preparation method and application |
| CN109007280A (en) * | 2018-08-20 | 2018-12-18 | 广州海诚生物科技有限公司 | A kind of biofermentation type function bighead feed |
| CN109007445A (en) * | 2018-08-20 | 2018-12-18 | 广州海诚生物科技有限公司 | A kind of biofermentation type function dace feed |
| CN109965083A (en) * | 2019-04-17 | 2019-07-05 | 佛山播恩生物科技有限公司 | A kind of depth fermented feed production method containing two kinds of high-content organic acids |
| CN111269850A (en) * | 2020-01-20 | 2020-06-12 | 吉林农业大学 | Pediococcus pentosaceus PP04 with high adhesion capability and blood fat reducing effect |
| CN111269850B (en) * | 2020-01-20 | 2022-07-22 | 吉林农业大学 | Pediococcus pentosaceus PP04 with high adhesion capability and blood fat reducing effect |
| CN111436526A (en) * | 2020-04-01 | 2020-07-24 | 浙江大学 | Preparation method and application of fermented rice bran meal with bacterium enzyme for improving growth performance of fattening pigs |
| CN112899200A (en) * | 2021-03-04 | 2021-06-04 | 江西格力特农牧发展有限公司 | Constant-temperature fermented liquid lactic acid bacteria and preparation method and application thereof |
| CN113897312A (en) * | 2021-10-09 | 2022-01-07 | 中国科学院成都生物研究所 | Preparation and application of animal feeding microbial inoculum |
| CN115152893A (en) * | 2022-07-04 | 2022-10-11 | 广西农业职业技术大学 | Compound probiotic composition for Qingyuan spicy-feather chicks and preparation method thereof |
| CN117016691A (en) * | 2023-08-09 | 2023-11-10 | 宁夏三十六度生物科技有限公司 | Probiotic feed additive for improving pork quality |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104106726A (en) | New probiotic, and probiotic fermentation feed prepared by using it | |
| CN102630852B (en) | Fermented broiler feed and preparation method thereof | |
| CN102041238B (en) | Lactobacillus plantarum, method for fermenting and preparing bacteriocin of Lactobacillus plantarum, and application of Lactobacillus plantarum and bacteriocin | |
| CN101278702B (en) | Novel microbial feed additive and method of producing the same | |
| CN105475624A (en) | Microbial fermentation feed as well as production method and application thereof | |
| CN104651268A (en) | Lactobacillus plantarum and application thereof | |
| CN109563472A (en) | Bacillus composition and method for ruminant | |
| CN101971920B (en) | Porcine lactobacillus reuteri lyophilized preparation and preparation method thereof | |
| CN101690540A (en) | Method for preparing composite protein feed by mixed fermentation | |
| CN102936568B (en) | Solid fermentation inoculum of chicken-origin bacillus subtilis and pediococcus pentosaceus and preparation method and application of solid fermentation inoculum | |
| CN105219669A (en) | There is resistance of oxidation, promote the bacillus amyloliquefaciens TL that growth of meat chicken is grown | |
| CN104673726A (en) | Porcine lactobacillus acidophilus freeze-drying preparation and application thereof | |
| KR20090030661A (en) | A feed additive for raising fishes, a feed composition, and a breeding method thereof | |
| CN102965316B (en) | Bacteriocin containing class and the feed addictive of Pediococcus acidilactici, premix and batch | |
| CN109924360A (en) | One boar fermentation compound feed and preparation method thereof | |
| CN102517227A (en) | Enterococcus faecalis and applications and feed additive and leavening agent thereof | |
| CN104605138A (en) | Poultry feed additive containing fermented Chinese yew, and preparation method and applications thereof | |
| Zhong et al. | Mixed culture of probiotics on a solid-state medium: An efficient method to produce an affordable probiotic feed additive | |
| CN103829040B (en) | A kind of with the method for Chen Xiangcha and bacillus subtilis composite fermentation dregs of beans and application | |
| CN103255082B (en) | One plant of excellent bacillus coagulans and its application for growing and fattening pigs cultivation | |
| KR20190047756A (en) | A composition comprising lactobacillus probiotics and a functional feed additive comprising the same | |
| KR101670955B1 (en) | Feed for farming-fish and Farming-fish farmed using that | |
| CN106811429A (en) | The application of one bacillus subtilis strain and its feed addictive and feed | |
| CN110946211A (en) | Fermented wheat bran and preparation method and application thereof | |
| CN109699813A (en) | It is a kind of to improve the solid state fermentation complete diet pellet for fattening meat quality and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20141022 |