CN103087548B - Method for extracting trichosanthes kirilowii maxim uranidin - Google Patents
Method for extracting trichosanthes kirilowii maxim uranidin Download PDFInfo
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- CN103087548B CN103087548B CN201310033406.5A CN201310033406A CN103087548B CN 103087548 B CN103087548 B CN 103087548B CN 201310033406 A CN201310033406 A CN 201310033406A CN 103087548 B CN103087548 B CN 103087548B
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- snakegourd
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- uranidin
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Abstract
The invention relates to a method for extracting trichosanthes kirilowii maxim uranidin, and solves the problems of dissolving difficulty, extracting difficulty and low extraction rate of the trichosanthes kirilowii maxim uranidin. The method comprises the steps of taking trichosanthes kirilowii maxim, removing seeds and skin; crushing trichosanthes kirilowii crumb, adding deionized water to mix; adjusting a pH value by citric acid; adding pectinase and cellulose; carrying out enzymolysis, filtering in vacuum to obtain a filter cake; adding ethanol to dissolve; bathing in water for 90 minutes at 50 DEG C; filtering to obtain filtrate; concentrating the filtrate by reduced pressure and drying in vacuum. The cell walls of colloid and cellulose are destroyed by a two-enzyme method, so that the uranidin in each cell can be released, so as to easily extract and improve the yield. The viscosity of the extracting solution can be reduced, and the extracting solution is easy to filter.
Description
Technical field
The invention belongs to natural plant pigment and extract preparing technical field, be specifically related to a kind of extracting method of snakegourd yellow pigment.
Background technology
Snake gourd Curcurbitaceae herbaceous perennial vine plant, all there is distribution national most areas.The yellow pigment that contains xenthophylls and carotene in mature fruit has very strong resistance of oxidation, also be the raw material that is used for the treatment of ocular fundus pathology medicine, researching and developing natural, safe, multi-functional pigment is the developing direction of sending out of current food, medicine industry simultaneously.
Snakegourd is from ancient times to the present extensively planted by China, and its fruit, seed, root all can enter separately medicinal.The skin of mature fruit, taste is sweet, cold in nature, has moistening the lung and resolving the phlegm, falls effect of fiery cough-relieving.Its flesh major ingredient is pectin and cellulose family composition, and with general solvent extraction, most snakegourd yellow pigment is difficult to stripping.
Summary of the invention
Go out for solving snakegourd yellow pigment indissoluble, be difficult for extracting, the problem that extraction yield is low, the invention provides a kind of extracting method of snakegourd yellow pigment.
Technical scheme of the present invention is: a kind of extracting method of snakegourd yellow pigment, get snakegourd, remove seed, peeling, by snakegourd flesh, pulverize, obtain feed liquid, add deionized water, the weightmeasurement ratio of feed liquid and deionized water is that (described bulking value refers to that feed liquid is in g to 1:15, deionized water is in ml), after mixing, citric acid adjust pH=4.0～6.0 that are 10% by mass concentration again, obtain compound, add the polygalacturonase of feed liquid weight 0.1～0.5% and the cellulase of feed liquid weight 0.3-1.0%, then, enzymolysis 3 hours under the condition of 50 DEG C～60 DEG C, vacuum filtration, obtain filter cake, filter cake is pigment part, adding mass concentration is 95% dissolve with ethanol, the weightmeasurement ratio of feed liquid and 95% ethanol is that (described bulking value refers to that feed liquid is in g to 1:20, 95% ethanol is in ml), 50 DEG C of water-baths 90 minutes, filter, obtain filtrate, filtrate decompression is concentrated, vacuum-drying, obtain.
The enzyme activity of described polygalacturonase is 50,000 u/g.
The enzyme activity of described polygalacturonase is 10,000 u/g.
The beneficial effect that the present invention is obtained
The present invention fully discharges 90% water-fast pigment in snakegourd flesh by double-enzyme method, solves the very low defect of traditional extraction process extraction yield.
It is mainly colloid and cellulosic material that snakegourd flesh goes after seed, and pigment is wrapped wherein.The present invention adopts double-enzyme method to destroy colloid and cellulosic cell walls, yellow pigment in cell can be discharged, thereby be easy to extract, and improves yield.And can reduce the viscosity of extracting solution, easily filter.
The invention solves the problem that in snakegourd flesh, yellow pigment is difficult to stripping, pectin substance in flesh, fibrination, is wrapped in pigment, causes the low technical barrier of extraction yield of snakegourd yellow pigment.
Specific embodiment
Embodiment 1
Get snakegourd, remove seed, peeling, snakegourd flesh is put into food-liquid grinder to be pulverized, obtain feed liquid, take feed liquid 10g, add 150ml deionized water, after mixing, be that 10% citric acid is adjusted to pH value=4.0 by mass concentration again, obtain compound, then add the cellulase of 0.05g polygalacturonase and 0.1g, enzymolysis 3 hours under the condition of 50 DEG C, vacuum filtration, filter cake is pigment part, adding 200ml mass concentration is 95% dissolve with ethanol, put in water-bath, 50 DEG C are heated 90 minutes, remove by filter impurity, get filtrate 1ml, be settled to 25ml with the ethanol that mass concentration is 95%, at 438nm place, survey its light absorption value A=0.72, residual filtrate concentrating under reduced pressure reclaims after ethanol, vacuum-drying obtains finished product 1.1g, one time extraction yield is 11%.
The enzyme activity of described polygalacturonase is 50,000 u/g.
The enzyme activity of described polygalacturonase is 10,000 u/g.
Embodiment 2
Get snakegourd, peeling, snakegourd flesh is put into food-liquid grinder to be pulverized, obtain feed liquid, take feed liquid 10g, add 150ml deionized water, after mixing, be that 10% citric acid is adjusted to pH value=5.3 by mass concentration again, obtain compound, then add the cellulase of 0.03g polygalacturonase and 0.07g, enzymolysis 3 hours under the condition of 60 DEG C, vacuum filtration, filter cake is pigment part, adding 200ml mass concentration is 95% dissolve with ethanol, put in water-bath, 50 DEG C are heated 90 minutes, remove by filter impurity, get filtrate 1ml, be settled to 25ml with the ethanol that mass concentration is 95%, at 438nm place, survey its light absorption value A=0.65, residual filtrate concentrating under reduced pressure reclaims after ethanol, vacuum-drying, 0.89g gets product, one time extraction yield is 8.9%.
The enzyme activity of described polygalacturonase is 50,000 u/g.
The enzyme activity of described polygalacturonase is 10,000 u/g.
Embodiment 3
Get snakegourd, remove seed, peeling, snakegourd flesh is put into food-liquid grinder to be pulverized, obtain feed liquid, take feed liquid 10g, add 150ml deionized water, after mixing, be that 10% citric acid is adjusted to pH value=6.0 by mass concentration again, then add the cellulase of 0.01g polygalacturonase and 0.03g, enzymolysis 3 hours under the condition of 55 DEG C, vacuum filtration, filter cake is pigment part, adding 200ml mass concentration is 95% dissolve with ethanol, put in water-bath, 50 DEG C are heated 90 minutes, remove by filter impurity, filter, get filtrate 1ml, be settled to 25ml with the ethanol that mass concentration is 95%, at 438nm place, measure its light absorption value A=0.66, after residual filtrate concentrating under reduced pressure, vacuum-drying obtains finished product 0.92g, one time extraction yield is 9.2%.
The enzyme activity of described polygalacturonase is 50,000 u/g.
The enzyme activity of described polygalacturonase is 10,000 u/g.
Comparison example
Get snakegourd flesh slag and put into food-liquid grinder and pulverize, take snakegourd flesh slurry 10g, add deionized water by the solid-liquid ratio of 1g:15ml, be adjusted to PH=5.8 with 10% citric acid, 50 DEG C ~ 60 DEG C heating in water bath 30 hours, cooling, lose pigment and separate out, vacuum filtration, sad filter.Filter cake takes out puts into 500ml tool plug triangular flask, add 50 DEG C of water-baths of ethanol 90 minutes of 200ml95%, filter, get filtrate 1ml, be settled to 25ml with 95% ethanol, at 438nm place, measure its light absorption value A=0.37, after residual filtrate concentrating under reduced pressure, vacuum-drying obtains finished product 0.56g, and one time extraction yield is 5.6%.
From above embodiment and comparative example, we can find out advantage of the present invention, and the present invention selects suitable enzyme dosage, PH, solid-liquid ratio, temperature and enzymolysis time, be easy to filter operation, and extraction yield is high, than without double-enzyme method, directly improve nearly one times of yield with 95% extraction using alcohol.
Claims (3)
1. the extracting method of a snakegourd yellow pigment, it is characterized in that: get snakegourd, remove seed, peeling, by snakegourd flesh, pulverize, obtain feed liquid, add deionized water, the weightmeasurement ratio of feed liquid and deionized water is 1:15, after mixing, citric acid adjust pH=4.0～6.0 that are 10% by mass concentration again, obtain compound, add the polygalacturonase of feed liquid weight 0.1～0.5% and the cellulase of feed liquid weight 0.3-1.0%, then, enzymolysis 3 hours under the condition of 50 DEG C～60 DEG C, vacuum filtration, obtain filter cake, filter cake is pigment part, adding mass concentration is 95% dissolve with ethanol, the weightmeasurement ratio of feed liquid and 95% ethanol is 1:20, 50 DEG C of water-baths 90 minutes, filter, obtain filtrate, filtrate decompression is concentrated, vacuum-drying, obtain.
2. the extracting method of snakegourd yellow pigment according to claim 1, is characterized in that, the enzyme activity of described polygalacturonase is 50,000 u/g.
3. the extracting method of snakegourd yellow pigment according to claim 1, is characterized in that, the enzyme activity of described polygalacturonase is 10,000 u/g.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103815509B (en) * | 2014-02-22 | 2016-01-20 | 南陵智联电子科技有限公司 | A kind of preparation method of Seeds of Trichosanthes kirilowii beverage |
| CN103860624B (en) * | 2014-03-26 | 2017-04-12 | 成都理工大学 | Method for removing fructus trichosanthis pulp by assist of biological enzyme |
| CN103923482B (en) * | 2014-04-10 | 2016-02-10 | 福建新华东食品有限公司 | The extracting method of Sea crab crab shell haematochrome |
| CN105238097A (en) * | 2015-11-06 | 2016-01-13 | 吉首大学 | Method for supercritical carbon dioxide extraction of green pigment in snakegourd seeds and pulp |
| CN109880400A (en) * | 2019-03-25 | 2019-06-14 | 潜山市天宝农业开发有限公司 | A kind of extraction process of the anti-oxidant uranidin of Snakegourd Fruit |
| CN111317691A (en) * | 2020-02-12 | 2020-06-23 | 南京中医药大学 | Trichosanthes kirilowii flesh total yellow pigment with whitening effect and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102166038A (en) * | 2011-03-15 | 2011-08-31 | 深圳波顿香料有限公司 | Method for extracting snakegourd peel, application of snakegourd peel extract to cigarettes and cigarettes |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102166038A (en) * | 2011-03-15 | 2011-08-31 | 深圳波顿香料有限公司 | Method for extracting snakegourd peel, application of snakegourd peel extract to cigarettes and cigarettes |
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| 孙体健等.栝楼黄色素的提取及性能研究.《中国食品卫生杂志》.2005,第17卷(第03期),228-231. |
| 文廷刚等.瓜蒌皮色素提取方法及其理化性质研究.《食品科学》.2009,第30卷(第05期),32-35. |
| 栝楼黄色素的提取及性能研究;孙体健等;《中国食品卫生杂志》;20050530;第17卷(第03期);228-231 * |
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