CN102060580A - Preparation of humic acid biological organic fertilizer from composite biological fungi - Google Patents
Preparation of humic acid biological organic fertilizer from composite biological fungi Download PDFInfo
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- CN102060580A CN102060580A CN2009102162315A CN200910216231A CN102060580A CN 102060580 A CN102060580 A CN 102060580A CN 2009102162315 A CN2009102162315 A CN 2009102162315A CN 200910216231 A CN200910216231 A CN 200910216231A CN 102060580 A CN102060580 A CN 102060580A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a method for preparing a humic acid organic fertilizer from composite biological microbial fungi. The microbial humic acid organic fertilizer comprises the following components in percentage by weight: 15 to 20 percent of livestock and poultry manure, 50 to 55 percent of humic acid, 15 to 20 percent of organic ammonium, 10 to 15 percent of phosphate rock powder, 3 to 5 percent of potassic fertilizer, 2 to 3 percent of microbial fungi and 0.5 to 1 percent of trace elements. The composite microbial fungi specifically refer to bacillus laterosporus and bacillus subtilis. The method comprises the following steps of: 1, preparing the bacillus laterosporus and the bacillus subtilis into bacterial suspension on the inclined plane of a potato dextrose agar (PDA) culture medium and spraying the bacterial suspension onto a carrier so as to obtain spore powder; 2, uniformly mixing materials such as 15 to 20 percent of livestock and poultry manure, 50 to 55 percent of humic acid, 15 to 20 percent of organic ammonium, 10 to 15 percent of phosphate rock powder, 3 to 5 percent of potassic fertilizer and the like, fermenting, drying, cooling and sieving so as to obtain a humic acid organic fertilizer for later use; and 3, adding the bacillus laterosporus, the bacillus subtilis and the trace elements into the humic acid organic fertilizer, mixing uniformly so as to obtain a finished product and canning.
Description
Technical field:
The present invention relates to a kind of humic acid bio-organic fertilizer that utilizes the composite microbial bacteria preparation and preparation method thereof.
Background technology:
Whole world agriculture production just develops towards the direction of Organic farming, green agriculture, and this is a Development Trend of World Agriculture, also is the outlet of Agricultural Sustainable Development in China.Be engaged in organic grow, green agricultural product production, using microorganism organic fertilizer is obligato basis.After the China's entry into the WTO, can food safety, food nutrition still march on towards the problem that the Chinese common people of well-off society pay close attention to the most comprehensively, are that can the agricultural-food of large agricultural country China go to the world yet, incorporate the key of world commerce mighty torrent.But, long-term a large amount of use of traditional chemical fertilizer in agriculture production, though make the output of agricultural-food be greatly improved, but employed chemical fertilizer has 70% loss approximately or is fixed in the soil, cause soil compaction, environmental pollution, infringement human health, because soil long-term lacking organic matter causes the crop products quality to descend, seriously restricting the Sustainable development of agricultural simultaneously.Addressing these problems the most basic way is usage quantity, raising chemical fertilizer utilization ratio, the increase soil organism that reduces chemical fertilizer.Because the most fertilizers that use of China peasant still are traditional composting method, the composting time is long, fertilizer efficiency is low, contaminate environment, and the fertilizer that does not fully become thoroughly decomposed still is the main contagium that farm crop are propagated disease, insect pest, makes the especially disease and pest aggravation of greenhouse crops of farm crop.Therefore, thereby the utilization ratio that can improve quality of agricultural product after the humic acid bio-organic fertilizer that utilizes compound biological bacterium preparation of the present invention is used, improve Soil structure, improves chemical fertilizer reduces the chemical fertilizer usage quantity, reduce disease and pest, increasing both production and income, this has created the huge market space for the humic acid biological organic fertilizer.
Summary of the invention:
The object of the invention is to design a kind of composite microbial bacteria that utilizes and prepares the humic acid bio-organic fertilizer, and this fertilizer is that animal dung, humic acid, nitrogen, phosphorus, potassium, composite microbial bacteria and trace element etc. are composited under specific micro-aerobe fermentation processing condition by a certain percentage.It is used for farm crop, can play the effect that improves agricultural product quality, the structure of improving the soil, improves chemical fertilizer utilization ratio and organic fertilizer, the effect of reduce the harm of disease worm, increasing the yield per unit area.
By design of the present invention, the composition of this humic acid bio-organic fertilizer is: animal dung 15-20%, humic acids 50-55%, organic ammonium 15-20%, ground phosphate rock 10-15%, potash fertilizer 3-5%,, microbial bacteria 2-3%, micro-0.5-1%, by weight percentage the amount.
Each composition role is below described:
1, animal dung: animal dung comprises animal manure and brid guano two big classes.The livestock fecaluria is meant the movement of raise livestocks such as pig, horse, ox, sheep, and it contains abundant organic matter and various nutritive element.Brid guano is the movement of poultry such as chicken, duck, goose, and it contains abundant organic matter and nitrogen, phosphorus, potassium and other nutrition.Animal dung is behind microbial fermentation, and Xiang Benfei provides abundant organic matter and nutritive element.
3, humic acid: humic acid is the animals and plants remains, through decomposition and the conversion of microorganism, and a series of chemical process and a type organic matter that accumulates.It is the polymeric organic acid that is made of aromatic series and multiple functional group such as hydroxyl, carboxyl thereof, has functions such as good physiologically active and absorption, complexing, exchange, plays the effect of the structure of improving the soil, water conservation, guarantor's fertilizer, raising fertilizer comprehensive utilization ratio.It and inorganic nitrogen, phosphorus, potassium element complexing form ammonium humate, humic acid phosphorus, potassium humate, are the important components of organic fertilizer.
4, organic ammonium, ground phosphate rock, potash fertilizer: nitrogen, phosphorus, potassium nutrition are provided, and they and humic acid complexing form ammonium humate, humic acid phosphorus, potassium humate, are the important components of organic fertilizer.
5, composite microbial bacteria: bacillus laterosporus (Bacillus Laterosporus), Bacillus subtillis (Bacillus Subtilis), these two kinds of microbial bacterias survive in the microorganism organic fertilizer with the statospore form, have high temperature resistant, salt tolerant branch, anti-ridity unique effects.Microbial bacteria enters and produces a large amount of extracellular enzymes behind the soil growth and breeding and decompose organic matter in the soil, nutrition directly is provided for crop; Meta-bolites not only can stimulate plant growth, and can also play prevention and elimination of disease and pests; A large amount of beneficial microorganisms occupies advantage at the crop rhizosphere ecosystem, and harmful microorganism is played the effect of " competition suppresses ", helps the healthy and strong growth of crop.
The present invention is realized by following three step technical schemes:
1, the preparation method of bacillus laterosporus, Bacillus subtillis spore powder
1.1 primary inclined plane is cultivated: Side spore genus bacillus, subtilis are inoculated on the inclined-plane of PDA substratum 30 ℃ and cultivate after 24 hours and make bacteria suspension.
1.2 secondary seed is cultivated: in the PDA liquid nutrient medium, (the shaking table revolution is 160 rev/mins) cultivated in concussion to 1% inoculum size, cultivates 48-72 hour for 25-30 ℃ with the bacterial suspension inoculation for preparing in proportion.
1.3 the preparation of spore powder: cultured seed liquid is sprayed on the carrier, and bake drying packages spare below 80 ℃.Detect gemma quantity, contain the gemma number at 50-180 hundred million/gram.
2, the preparation method of humic acid organic fertilizer material
2.1 raw material is prepared:
2.1.1 animal dung: comprise that chicken and duck excrement, pig cow dung etc. dehydrate.
2.1.2 stalk: be ground into length and be 0.5-2 centimetre straw powder.
2.1.3 humic acid: peat or brown coal humic acid are broken up drying, are ground into the 60-80 order with roller mill with crusher, and adding a kind of special preparation, that humic acid is activated after drying is standby.
2.1.5 mineral manure is prepared: it is standby to buy vitriolate of tartar, Repone K, ground phosphate rock, bicarbonate of ammonia, ammonium chloride, ammonium sulfate and organic ammonium etc.
2.2 preparation of raw material: animal dung 15-20%, humic acid 50-55%, organic ammonium 15-20%, ground phosphate rock 10-15%, potash fertilizer 3-5% add enzymatic microorganism, and the amount batching is mixed thoroughly by weight percentage, regulates to contain moisture in the 55-60% scope, ferments to fermenter.
2.3 fermentation: will get above-mentioned each material ready and put into fermenter, and add the lignocellulose bacterium, throwing is turned in mechanize, with opening material, increase amount of oxygen.One time fermentation requirement 12-15 days, the fermentation top temperature must reach 65-75 ℃ of degree.Secondary Fermentation adopts to concentrate stores up 20-30 days micro-aerobe fermentations, and temperature is controlled at 48-52 ℃ of degree.
2.4 oven dry, pulverizing: the material that will fully ferment is through the tunnel kiln drying, and temperature is controlled at 80 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches the 40-60 order, and is standby.
3, humic acid bio-organic fertilizer preparation
The Side spore genus bacillus, the subtilis spore powder (weight ratio 1: 1) that prepare are added in the humic acid organic fertilizer material in the ratio of 0.05-0.1%, guarantee total viable count reach 2,000 ten thousand/gram more than, each bacterium number of viable reach 1,000 ten thousand/gram more than, add trace elements such as zinc sulfate, magnesium, molybdenum again, under the forced-ventilated stirrer, fully mix canned one-tenth commodity humic acid biological organic fertilizer thoroughly.
Claims (2)
1. this component for preparing the humic acid bio-organic fertilizer with composite microbial bacteria is: animal dung 15-20%, humic acids 50-55%, organic ammonium 15-20%, ground phosphate rock 10-15%, potash fertilizer 3-5%,, composite microbial bacteria 2-3%, micro-0.5-1%, by weight percentage the amount.
2. composite microbial bacteria according to claim 1 refers in particular to: 1. bacillus laterosporus (Bacillus Laterosporus), 2. subtilis (Bacillus Subtilis).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2009102162315A CN102060580A (en) | 2009-11-17 | 2009-11-17 | Preparation of humic acid biological organic fertilizer from composite biological fungi |
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| CN2009102162315A CN102060580A (en) | 2009-11-17 | 2009-11-17 | Preparation of humic acid biological organic fertilizer from composite biological fungi |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102424615A (en) * | 2011-09-05 | 2012-04-25 | 麻林涛 | Special compound microbial fertilizer for tobacco and preparation method thereof |
| CN102424627A (en) * | 2011-09-05 | 2012-04-25 | 麻林涛 | Special composite microbial fertilizer for vegetables and preparation method thereof |
| CN102816021A (en) * | 2012-07-31 | 2012-12-12 | 马鞍山科邦生态肥有限公司 | Special fertilizer for rape base fertilizer |
| CN103030448A (en) * | 2012-08-09 | 2013-04-10 | 上海联业农业科技有限公司 | Biological organic fertilizer applied to Gannan red and yellow soil region and preparation method thereof |
| CN103508774A (en) * | 2013-09-22 | 2014-01-15 | 临汾市力澜生物科技有限公司 | Method for preparing organic fertilizer by mixing livestock excrement and humic acid |
| CN103965916A (en) * | 2014-05-07 | 2014-08-06 | 苟俊 | Soil restoration agent, method for preparing soil restoration agent and method for restoring soil |
| CN104945150A (en) * | 2015-07-06 | 2015-09-30 | 合肥福泉现代农业科技有限公司 | Morchella angusticeps high-efficiency culture medium capable of enhancing extracellular SOD (superoxide dismutase) activity and preparation method thereof |
| CN107098769A (en) * | 2017-06-23 | 2017-08-29 | 江苏祥豪实业股份有限公司 | A kind of organic-inorganic compound fertilizer |
| CN109160845A (en) * | 2018-09-25 | 2019-01-08 | 南通蛇类治疗研究所 | The agriculture compound trace element fertilizer that agriculture and industry waste is produced |
| CN110950711A (en) * | 2019-12-20 | 2020-04-03 | 贵州芭田生态工程有限公司 | Biological organic fertilizer and preparation method and application thereof |
-
2009
- 2009-11-17 CN CN2009102162315A patent/CN102060580A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102424615A (en) * | 2011-09-05 | 2012-04-25 | 麻林涛 | Special compound microbial fertilizer for tobacco and preparation method thereof |
| CN102424627A (en) * | 2011-09-05 | 2012-04-25 | 麻林涛 | Special composite microbial fertilizer for vegetables and preparation method thereof |
| CN102816021A (en) * | 2012-07-31 | 2012-12-12 | 马鞍山科邦生态肥有限公司 | Special fertilizer for rape base fertilizer |
| CN102816021B (en) * | 2012-07-31 | 2014-04-02 | 马鞍山科邦生态肥有限公司 | Special fertilizer for rape base fertilizer |
| CN103030448A (en) * | 2012-08-09 | 2013-04-10 | 上海联业农业科技有限公司 | Biological organic fertilizer applied to Gannan red and yellow soil region and preparation method thereof |
| CN103030448B (en) * | 2012-08-09 | 2014-11-19 | 上海联业农业科技有限公司 | Biological organic fertilizer applied to Gannan red and yellow soil region and preparation method thereof |
| CN103508774A (en) * | 2013-09-22 | 2014-01-15 | 临汾市力澜生物科技有限公司 | Method for preparing organic fertilizer by mixing livestock excrement and humic acid |
| CN103965916A (en) * | 2014-05-07 | 2014-08-06 | 苟俊 | Soil restoration agent, method for preparing soil restoration agent and method for restoring soil |
| CN104945150A (en) * | 2015-07-06 | 2015-09-30 | 合肥福泉现代农业科技有限公司 | Morchella angusticeps high-efficiency culture medium capable of enhancing extracellular SOD (superoxide dismutase) activity and preparation method thereof |
| CN107098769A (en) * | 2017-06-23 | 2017-08-29 | 江苏祥豪实业股份有限公司 | A kind of organic-inorganic compound fertilizer |
| CN109160845A (en) * | 2018-09-25 | 2019-01-08 | 南通蛇类治疗研究所 | The agriculture compound trace element fertilizer that agriculture and industry waste is produced |
| CN110950711A (en) * | 2019-12-20 | 2020-04-03 | 贵州芭田生态工程有限公司 | Biological organic fertilizer and preparation method and application thereof |
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Application publication date: 20110518 |