CN101569311B - Photolysis-resistant bactericidal suspoemulsion and preparation and use methods thereof - Google Patents
Photolysis-resistant bactericidal suspoemulsion and preparation and use methods thereof Download PDFInfo
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- CN101569311B CN101569311B CN2009100869590A CN200910086959A CN101569311B CN 101569311 B CN101569311 B CN 101569311B CN 2009100869590 A CN2009100869590 A CN 2009100869590A CN 200910086959 A CN200910086959 A CN 200910086959A CN 101569311 B CN101569311 B CN 101569311B
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- suspension emulsion
- photodissociation
- natamycin
- bactericidal
- plant
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Abstract
The invention discloses a photolysis-resistant bactericidal suspoemulsion and a preparation method thereof, belonging to the field of biopesticide processing and application. The invention also discloses a use method of the suspoemulsion. The suspending agent not only can effectively prevent and treat the soil-borne fungal diseases of plants, but also can effectively prevent and treat the fungal diseases of overground tissues of the plants, has the characteristics of wide bactericidal spectrum, good bactericidal effect and low cost, and has good development and application prospects.
Description
Technical field
The invention belongs to biopesticide processing and application, be specifically related to a kind of anti-photodissociation bactericidal suspension emulsion and preparation and method for using.
Background technology
Natamycin (Natamycin) is claimed Natta mycin or Natamycin again, and molecular structural formula is seen Fig. 1; It can obligate suppress saccharomycete and mould, and its mechanism of action is that it acts on the somatic cells film, with the ergosterol effect that contains in the plasma membrane; The damaging cells plasma membrane causes the leakage of intracellular matter, and (Tanaka believes the man thereby play bactericidal action; Beijing Science Press, 1977).At present; Known streptomyces chatanoogensis (Streptomyces chattanovgensis), Natal streptomycete (S.natalensis), brown yellow spore streptomycete (S.gilvosporeus) (Lu Guoying; Feed industry; 2005,26 (11): the antibacterial activity product that 28-31) metabolism produced is a natamycin.Recently; The Beijing City Agriculture and Forestry Institute is planted ring institute and is successfully utilized actinomycetes streptomyces lydicus A01 (Streptomycesl lydicus) to obtain the concentrate (Chinese patent " streptomyces lydicus and the application thereof of strain product natamycin " of antibacterial activity product natamycin; Application number: 200710187435.1), and prove that its antibacterial substance is natamycin (seeing accompanying drawing 4).
Natamycin has following advantage as antibacterial agent, has low dosage (10
-6The order of magnitude), high efficiency, the characteristics that the antibacterial action time is long are a kind of efficient, safe natural biological property antibacterial agents, bacteriostasis is stronger about 50 times than sorbic acid, and uses for several years running and be difficult for also causing that the target fungi forms resistance.The safety that natamycin is checked 3 times respectively at nineteen sixty-eight, 1976 and calendar year 2001 by FAO/WHO Expert C-on Food Additive confirms that its acceptable intake every day (ADI) is: the 0.3mg/kg body weight/day.Because natamycin is mainly used in surface treatment, though a large amount of edible product that is processed can not reach yet the people's amount of taking the photograph average day (Wang Guifang, Zhao Shaohua, Food Additives Used in China, 2006:2:144-151).Therefore, natamycin is very safe to people and animals, at present in beverage, feed, food and pharmaceutically extensive use, but the kind fungus diseases (Ceng Guangran, Jilin agricultural science, 1989, (2): 28-36) that in agricultural production, only limit to be applied to prevent and treat cotton and beans.
Natamycin not on agricultural the reason of extensive use have two: the one, be subject to ultraviolet ray influence when being used to prevent and treat the plant shoot disease and decompose.This is because the natamycin molecule is a kind of polyene structure, and this makes natamycin relatively more responsive to ultraviolet ray; The 2nd, the main commodity formulation of present natamycin is 50% crystal, it be metabolite through microorganism through concentrating, purify, cost is higher, it is not agricultural not suit.
At present, in the formulations of pesticide processing at home and abroad, agricultural chemicals suspension emulsion (SE) is used for the weed killer herbicide processing more than 2 kinds or 2 kinds more, is the good formulation that solid pesticide is become mixture with former oily agricultural chemicals hybrid process.Because mostly UV absorbers is directly to be processed into the solid of suspending agent, but is soluble in organic solvent, and mostly the antibacterial activity product of microorganism is water insoluble solid, therefore they being processed into suspension emulsion is a kind of good selection.But this formulation is applied to the processing of biopesticide does not appear in the newspapers as yet.
Summary of the invention
The present invention has overcome above-mentioned shortcoming; A kind of anti-photodissociation bactericidal suspension emulsion is provided; This suspension emulsion can directly utilize the concentrate of streptomyces antibacterial activated product, adds UV absorbers, processes the agricultural chemicals suspension emulsion; This medicament can effectively be prevented and treated plant soil-borne diseases, also can effectively prevent and treat plant shoot and organize disease.
The present invention also provides the preparation method of above-mentioned anti-photodissociation bactericidal suspension emulsion.
The present invention also provides the method for using of above-mentioned anti-photodissociation bactericidal suspension emulsion.
A kind of anti-photodissociation bactericidal suspension emulsion, active component is a natamycin, each component and mass percent thereof are: natamycin 0.01%～6%; UV absorbers 3%～30%; Toluene or xylol 3%～30%; Emulsifier 3%～15%; Antiprecipitant 0.5%～15%; Surplus is a water.The component of suspension emulsion is not limited to said components, also comprises in the following component one or more, antifreeze, antifoaming agent and pH adjustment agent, and its using dosage is this area routine dose.Wherein, antifreeze is like ethylene glycol, propane diols, glycerine, urea etc.; Antifoaming agent is like polyethers, organic copper silicon class, C
8～10Fatty alcohol, C
10～20Representative examples of saturated aliphatic carboxylic and ester class thereof, ester-ether type compound etc.; PH adjusts agent, like acetic acid, hydrochloric acid, sodium hydroxide, potassium hydroxide, ammonium hydroxide, ammonium salt, triethanol ammonium etc.
Preferred following component of said suspension emulsion and mass percent thereof: natamycin 0.05%～5%; UV absorbers 5%～20%; Toluene or xylol 5%～20%; Emulsifier 3%～10%; Antiprecipitant 1%～10%; Surplus is a water.
The source of said natamycin is streptomyces lydicus A01, brown yellow spore streptomycete, Natal streptomycete or antibiotic concentrate that streptomyces chatanoogensis produced.
Said antibiotic concentrate is meant a kind of antibacterial activity product that comes from streptomyces lydicus A01, streptomyces chatanoogensis, Natal streptomycete, brown yellow spore streptomycete; Product warp 0.45 μ m filtering with microporous membrane also concentrates and obtains antibiotic concentrate, wherein contains the active component natamycin.
The preparation method of above-mentioned streptomyces lydicus A01 antibacterial activity product is with reference to patent " streptomyces lydicus and the application thereof of natamycin produced in a strain ", and application number is 200710187435.1.
The preparation method of the antibacterial activity product of above-mentioned brown yellow spore streptomycete is with reference to patent " Natamycinrecovery " (Raghoenath, Dilipkoemar, Webbers, et al.USP:6150143,2000-11～21); " Fermentation process for producing Natamycin with additional carbon andnitrogen " (Eilsenschink M A, Mils J R, MichaelA.USP:5686273,1997); " preparation of kind bacterium and the propagation method of natamycin " (M A Ai Senshenke .CNP:1071460A, 1993); " pH through the control fermentation medium is to improve the throughput rate of natamycin " (M A Ai Senshenke .CNP:1072959A, 1993); " continuous fermentation method production natamycin " (P T Mancur Olson .CNP:1070688A, 1993).
The preparation method of the antibacterial activity product of above-mentioned Natal streptomycete is except the preparation method with reference to the antibacterial activity product of brown yellow spore streptomycete, but reference literature (Enshasy H A, Farid M A.J. also; BasicMicrobiol.; 2000,40 (5-6), 333-342); (Enshasy H A, Faird M A.J., Basic Microbiol., 2000,40 (3), 157-166).
The preparation method of the antibacterial activity product of above-mentioned streptomyces chatanoogensis is except with reference to the said method, but reference literature (Zheng Fenge etc., food industry science and technology, 2008 (29), 8:159-160,172).
Said UV absorbers is UV-234 and UV-531, and its molecular structure is seen accompanying drawing 2 and 3 respectively, UV-234 be 2-(2 '-hydroxyl-3 ', 5 ' two (a, a-dimethyl benzyl) phenyl) BTA, UV-531 is a UV-531.
Said emulsifier is a farming breast 600
#, farming breast 601
#, farming breast 602
#With farming breast 500
#In one or more.
Said antiprecipitant (claiming thickener or suspension stabilizer again) is one or more in sodium carboxymethylcellulose, bentonite, xanthan gum and the Magnesiumaluminumsilicate.
The controlling object of said suspension emulsion is a fungal disease; Said disease is graw mold of tomato (Botrytiscinerea), capsicum gray mold (Botrytis cinerea), grey mould of egg plant (Botrytis cinerea), grape grey mould (Botrytis cinerea), the corn northern leaf blight (Exserohilum turcicum), the rotten mildew (Pythium aphanidermatum) of melon and fruit, eggplant early blight (Alternaria solani), wheat sharp eyespot (Rhizoctonia cerealis), wheat scab (Fusarium graminearum), rice blast (Pyriculariaoryzae), pea root rot (Fusarium solani f.sp.pisi), cladosporium leaf and fruit mould of tomato (Cladosporiumfulvum), watermelon blight (Fusarium oxysporum f.sp.niveum), cucumber fusarium axysporum (Fusariumoxysporum f.sp.cucumerinum), lily root rot (Rhizoctonia solani), plum brown rot (Monilinia fructicola), peach brown rot (Monilinia fructicola), peach fusarium wilt (Fusariumoxysporum f.sp.persica), celery septoria disease (Septoria apii), pepper anthracnose (Colletotrichumcapsici), wild cabbage fusarium wilt (Fusarium oxysporum Schl.f.sp.conglutinans), apple zonate spot (Alternaria mali), cotton verticillium wilt (Verticillium dahliae), cotton wilt (Fusariumoxysporum f.sp.vasinfectum) etc., but is not limited to above-mentioned fungal disease.
A kind of preparation method of anti-photodissociation bactericidal suspension emulsion, the concrete operations step is following: prepare suspension emulsion according to the above ratio, take by weighing UV absorbers earlier; It is dissolved in toluene or the xylol, adds emulsifier after the heating for dissolving, stir; Obtain solution 1, it is joined in the natamycin, the limit edged stirs; Until mixing, add antiprecipitant then and water stirs again, put into grinder be ground to 95% grain fineness less than 5 μ m till.Preferred 50～70 ℃ of temperature when wherein, solution 1 joins natamycin.
A kind of preparation method of anti-photodissociation bactericidal suspension emulsion also comprises in the said method adding adding antifreeze, antifoaming agent or pH adjustment agent more respectively behind the antiprecipitant and water stirs.
A kind of method for using of anti-photodissociation bactericidal suspension emulsion; The concrete operations step is following: fall ill before or their early stage plant; Press the working concentration 10～100ppm of active component natamycin; Suspension emulsion is converted water be mixed with dilution, adopt spray-on process or root-pouring method that it is sprayed on plant surface or imposes on plant root.Wherein, the intensity of illumination during sprinkling is preferably less than 7543LX, at dusk or the cloudy day promptly.
Above-mentioned plant surface is meant the overground part tissues such as surface of leaf, stem and the fruit of plant.
The present invention has the following advantages:
(1) compare with the medicament that does not add UV absorbers, the stability that has the suspension emulsion active component of UV absorbers is improved largely, and organizes disease on the ground thereby make medicament under the low light level, can effectively prevent and treat plant.
(2) the invention enables the concentrate of microbial antibacterial activated product can directly be processed into pesticidal preparations, and need not pass through purification process, practiced thrift production cost thus, for medicament extensive use in agricultural production is laid a good foundation.
(3) be crop production, especially vegetables produce, and safe biopesticide kind is provided, and have ensured people health.
Description of drawings
Fig. 1 is the chemical constitution of natamycin;
Fig. 2 is a ultra-violet absorber UV-234 chemical constitution;
Fig. 3 is the ultraviolet absorbent UV-531 chemical constitution;
Fig. 4 is the uv absorption spectra of streptomyces lydicus A01 active substance (A) and natamycin (B).
Embodiment
Below be raw material, reagent and the bacterial classification that relates among the embodiment:
1 strains tested
Streptomyces lydicus A01 (Streptomycesl lydicus) (CGMCC No.1653); Be called for short strains A 01 in following examples; Plant ring institute by the Beijing City Agriculture and Forestry Institute and from the Beijing suburb vegetable soil, separate acquisition, be preserved in the biocontrol of plant disease research department and China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) of this research institute.Strains A 01 transferred on the Gause I slant medium 28 ℃ cultivated 10～14 days; Cultured inclined-plane can be deposited subsequent use 4 ℃ of short-terms, and-20 ℃ of long preservation are subsequent use (sees Chinese invention patent " streptomyces lydicus and the application thereof of natamycin produced in a strain " (application number: 200710187435.1) but simultaneously its ripe fresh spore is put in 20% glycerine.
Plant pathogenic fungi: verticillium dahliae (Verticillium dahliae); Cotton-wilt fusarium (Fusarium oxysporum f.sp.vasinfectum); Botrytis cinerea (Botrytis cinerea); Capsicum ash arrhizus bacteria (Botrytis cinerea); Grey mould of egg plant bacterium (Botrytis cinerea); Grape grey mould bacterium (Botrytis cinerea); Exserohilum turcicum (Exserohilum turcicum); Melon and fruit pythium spp (Pythiumaphanidermatum); Eggplant early blight bacterium (Alternaria solani); Rhizoctonia cerealis (Rhizoctoniacerealis); Fusarium graminearum (Fusarium graminearum); Pyricularia oryzae (Pyriculariaoryzae); Pea pine root fungus (Fusarium solani f.sp.pisi); Cladosporium leaf and fruit mould of tomato bacterium (Cladosporiumfulvum); Withered germ of water-melon (Fusarium oxysporum f.sp.niveum); Cucumber fusarium axysporum (Fusarium oxysporum f.sp.cucumerinum); Lily pine root fungus (Rhizoctonia solani); Plum brown rot germ (Monilinia.fructicola); Monilinia fructicola (Monilinia fructicola); Peach wilt (Fusarium oxysporum f.sp.persica); Celery septoria disease bacterium (Septoria apii); Pepper anthracnose bacterium (Colletotrichum capsici); Wild cabbage wilt (Fusarium oxysporum Schl.f.sp.conglutinans); Apple zonate spot bacterium (Alternaria mali).
Saccharomycete: saccharomyces cerevisiae (Saccharomyces cerevisiae).
Above plant pathogenic fungi is to plant ring by plant pathology system of China Agricultural University and Beijing City Agriculture and Forestry Institute to provide, and saccharomyces cerevisiae is available from Chinese agriculture microorganism fungus kind preservation administrative center (ACCC).
2 supply examination medium and solution
The PDA medium: potato 200g, clean and be cut into 1cm
3Fritter boils behind about 30min with four layers of filtered through gauze, and filtrating complements to 1000ml with distilled water, adds glucose 20g, agar 15g, pH nature, 121 ℃ of sterilization 30min.
Gause I medium: soluble starch 20g, NaCl 0.5g, KNO
31g, FeSO
47H
2O0.01g, K
2HPO
40.5g, MgSO
47H
2O 0.5g, agar 20g, distilled water 1000ml, pH 7.2～7.4,121 ℃ of sterilization 30min.
Beef-protein medium: beef extract 3g, peptone 10g, NaCl 5g, agar 15g, distilled water 1000ml, pH 7.4～7.6,121 ℃ of sterilization 30min.
Physiological saline: NaCl 8.5g, distilled water 1000ml, 121 ℃ of sterilization 30min.
3 strains A, 01 concentrate
The preparation method is with reference to patent " streptomyces lydicus and the application thereof of natamycin produced in a strain ", and application number is 200710187435.1
4 other reagent and equipment
Ultra-violet absorber UV-234 and UV-531 are available from excellent constant force fine chemistry Co., Ltd in Beijing; Emulsifier series: farming breast 600
#, farming breast 601
#, farming breast 602
#, farming breast 500
#Solvent: xylol, toluene; Antiprecipitant: sodium carboxymethylcellulose (CMC), bentonite and Magnesiumaluminumsilicate; Antifreeze: urea; Antifoaming agent: bubble enemy (antifoaming agent GPE, polyethers); PH adjusts agent: 36% acetic acid; 1% polyoxin aqueous solution is available from Ludun Biological Products Co., Ltd., Shaanxi; QZM-1 type cone mill; Microscope (tool calibration connect order micrometer); Sprayer: (Matabi 121010Style 1.5); Illuminometer: (model: TES-1339, TES Electronic Industrial Corporation); Tomato variety: potted plant red (Beijing agricultural academy of sciences vegetables provide).
The preparation of embodiment 1 anti-photodissociation bactericidal suspension emulsion of the present invention
Component and quality proportioning by table 1～2 take by weighing each component preparation suspension emulsion of the present invention, take by weighing UV absorbers earlier, and it is dissolved in toluene or the xylol; Add emulsifier after the heating for dissolving; Stir, obtain solution 1, it is joined in strains A 01 concentrate; The limit edged stirs; Until mixing, add antiprecipitant, antifreeze, antifoaming agent, pH adjustment agent and water then successively and stir again, put into grinder be ground to 95% grain fineness less than 5 μ m till (measuring) with microscope.
Each constituent mass proportioning (one) of the anti-photodissociation bactericidal suspension emulsion of table 1
Each constituent mass proportioning (two) of the anti-photodissociation bactericidal suspension emulsion of table 2
-: do not contain this component in the expression suspension emulsion
The antimicrobial spectrum of embodiment 2 strains A 01 active metabolite is measured
(1) the dull and stereotyped bacteriostasis of viable bacteria body
Adopt dull and stereotyped face-off cultivation (Lu Suyun, Beijing Agricultural University's publication, 1993).Strains A 01 28 ℃ of constant temperature culture 14 days on the Gause I medium, plant pathogenic fungi 28 ℃ of constant temperature culture 4 days on the PDA flat board are made the agar block that carries disease germs from colony edge with the aseptic stainless steel card punch of diameter 0.7cm; With aseptic inoculation pin picking pathogen bacterium sheet, mycelia faces down and is inoculated on the PDA flat board, and the bacterium sheet is apart from culture dish edge 2cm; While is the agar bacterium sheet that carries disease germs of picking strains A 01 in kind; Placing apart from disease fungus bacterium sheet 4.00cm place, is contrast not connect the flat board that strains A 01 only connects pathogen, and each is handled three times and repeats; 25 ℃ of constant temperature culture 4 days are measured the antibacterial bandwidth between pathogen edge and strains A 01 colony edge.
(2) bacteriostasis of active substance in the zymotic fluid
Adopt the agar plate diffusion process.The test plant disease fungus is in 28 ℃ of constant temperature culture on the PDA inclined-plane after 7 days, scrapes to get its conidium and mycelium is put firmly fully vibration in the triangular flask that fills sterile glass beads (diameter 2.5mm) and sterile water, is made into 10
6The bacteria suspension of CFU/ml; Getting 200 μ l bacteria suspensions is uniformly coated on the PDA flat board (diameter 9cm); Make three holes with the aseptic stainless steel card punch of diameter 0.7cm is equidistant; In every hole, inject ferment filtrate 100 μ l then through the strains A 01 of 0.45 μ m filtering with microporous membrane sterilization; 25 ℃ of constant temperature culture 48h, the right-angled intersection method is measured the diameter of inhibition zone; Test plant pathogenetic bacteria and saccharomyces cerevisiae after cultivating 2 days on beef-protein medium and the PDA, are made into 10 with SPSS respectively
4The bacteria suspension of CFU/ml concentration; Get and fall to make flat board after 5ml bacteria suspension and 100ml are cooled to the PDA medium mixing about 45 ℃, each culture dish (diameter 9cm) is poured the 30ml mixed liquor into, after waiting to solidify; Make three holes with the aseptic stainless steel card punch of diameter 0.7cm is equidistant; In every hole, inject A01 ferment filtrate 100 μ l through 0.45 μ m filtering with microporous membrane degerming, 28 ℃ of constant temperature culture 48h, the right-angled intersection method is measured the diameter of inhibition zone.Each is handled three times and repeats.
From table 3, can find out; 01 pair of strains A supplies the 10 various plants disease funguses and the saccharomyces cerevisiae (S.cerevisiae) of examination to have very strong bacteriostatic activity; Wherein all more than 1.50cm, the highest is that antibacterial bandwidth reaches 2.57cm to pepper anthracnose bacterium (C.capsici) to the antibacterial bandwidth of the plant pathogenic fungi of 01 pair of confession examination of strains A; Its zymotic fluid is except to the antibacterial circle diameter of saccharomyces cerevisiae is below 3.00cm; Antibacterial circle diameter to other disease funguses has all reached more than the 3.00cm, and the strongest fungistatic effect is that diameter reaches 4.77cm to rhizoctonia cerealis (R.cerealis) and plum brown rot germ (M.fructicola).Explain that thus the active substance of strains A 01 generation has the antifungal activity of wide spectrum.
Table 3 strains A 01 bacteria inhibition assay result
Annotate: this content is not surveyed in "-" expression; Data are the mean value of 3 repetition numerical value in the table.
4. 3. suspension emulsion prevent and treat the control efficiency of tomato in plastic greenhouse gray mold among embodiment 3 embodiment 1 with suspension emulsion
3. suspension emulsion among the embodiment 1 is made as code name NUV234SE, and 4. suspension emulsion is made as code name NUV531SE.In booth, tomato seedling is bred high strong sprout of 8～10cm, move into high 15cm then, in the plastic flowerpot of diameter 13cm (every basin 1 strain), be cultured to the dispenser of tomato initial bloom stage.Each medicament is mixed with the soup that effective content is 20ppm, and its test is handled totally 5 kinds, sees shown in the table 5.Sprayer is adopted in dispenser, and every young plant spraying capacity is 33.3mL, repeats 3 times, and district's group is arranged at random.The spray medicine is placed on 1.5h in the booth, makes respectively to handle medicament and expose to sunlight, and zero-time is at 1 o'clock in afternoon, and the concluding time is 2:30 in afternoon.Measure 1 intensity of illumination when initial, measure 1 intensity of illumination during end, repeat 5 times.Average intensity of illumination is 23892LX when initial, and average intensity of illumination is 13586LX during end, and successively twice mean value is 18740LX.Inoculate after the solar radiation, it is botrytis cinerea (Botrytis cinerea) that sprayer, bacterial classification are also adopted in inoculation, and the spore concentration of suspension is 10
7～10
8CFU, every basin tomato seedling spraying capacity is 20mL.Placing temperature after the inoculation immediately is 20 ℃, and relative moisture is to preserve moisture in 70～100% the incubator to cultivate 48h, places plastic tunnel to cultivate then.1～14 day every poisoning situation of observing at a distance from 1 day after the dispenser; Investigated disease index, the incidence of disease and control efficiency in 7 days and 14 days after the dispenser, its computational methods are following:
The incidence of disease (the %)=morbidity number of sheets/investigate total number of sheets * 100
Disease index (%)=∑ [(the sick level number of sheets * represent numerical value)]/representative numerical value of heavy duty (total number of sheets of investigation * fall ill) * 100
Control efficiency (%)=(contrast disease index-processing disease index)/contrast disease index * 100
Each processing of observation in 1～14 day does not have any symptom of chemical damage to tomato after the dispenser.Visible from table 4; Investigated in 14 days behind the medicine; Adding the NUV234SE of ultra-violet absorber and the control efficiency of NUV531SE processing is 49.6%～50.9%; And the preventive effect of strains A 01 concentrate and polyoxin is respectively 9.8% and 4.6%, and NUV234SE and NUV531SE control efficiency obviously are superior to A01 concentrate and polyoxin, and have significant difference.There is significant difference in effect between them.
The 20ppm natamycin soup of the different medicaments of table 4 is prevented and treated the effect of tomato in plastic greenhouse gray mold
Annotate: data are the mean value of 3 repetitions in the table; Different letter representations behind the mean are through Duncan ' s multiple range test significant difference on the p=0.05 level.
Embodiment 4 different spraying time NUV234SE and NUV531SE prevent and treat the effect of tomato in plastic greenhouse gray mold
Each medicament is mixed with the soup that effective content is 20ppm, and its test is handled totally 8 kinds, sees shown in the table 6.First handles spraying time is at 1 o'clock in afternoon (pm.1), and being positioned over and receiving light application time in the booth is 3h, and average intensity of illumination is 25240LX when initial, and average intensity of illumination is 7543LX during end, and successively twice mean value is 16392LX.The second batch processing spraying time is at 4 o'clock in afternoon (pm.4), and average intensity of illumination is 7543LX when initial.Other test method is with embodiment 3.
Visible from table 5; Investigated in 7 days and 14 days after the dispenser; The preventive effect that sprayed A01 concentrate (not adding ultraviolet absorber) at 1 o'clock in afternoon is respectively 26.3% and 30.4%; The preventive effect of spray at 4 o'clock in afternoon medicine is respectively 62.5% and 67.4%, has significant difference between the two, and the effect of spray at 4 o'clock in afternoon medicine is superior to the effect of spray at 1 o'clock in afternoon medicine; The preventive effect that sprayed NUV234SE in the afternoon at 1 o'clock is respectively 84.1% and 83.2%, and the preventive effect of 4 o'clock spray medicines is respectively 93.4% and 95.3% in the afternoon, also has significant difference between the two, and the effect of spray at 4 o'clock in afternoon medicine is superior to the effect of spray at 1 o'clock in afternoon medicine; The effect of adding UV-531 is similar to UV-234.The result shows that the order of preventive effect good job is NUV234SE=NUV531SE>polyoxin>the A01 concentrate; The A01 concentrate control efficiency of adding UV absorbers is superior to single use A01 concentrate (not adding UV absorbers); NUV234SE and the NUV531SE result of use of (average intensity of illumination can remain on several hours less than 7543LX after being dispenser, like dusk or cloudy day) under the more weak condition of sunlight is superior to the result of use under the stronger condition of sunlight (noon).
Table 5 spraying time is prevented and treated the effect of tomato in plastic greenhouse gray mold to NUV234SE and NUV531SE
Annotate: data are the mean value of 3 repetitions in the table; Different letter representations behind the mean are through Duncan ' s multiple range test significant difference on the p=0.05 level.
Embodiment 5NUV234SE and the test of NUV531SE control watermelon blight
Select one in the serious plot of watermelon blight in former years in Daxing County, Beijing, watermelon density is 500 strain/mus, glad No. one of kind capital, and the soil texture is a sandy loam.Supply the reagent agent to be respectively NUV234SE, NUV531SE, A01 concentrate and 1% polyoxin aqueous solution, each medicament is mixed with the soup of 30ppm, and establishes the processing CK of a not dispenser, totally 5 kinds of processing.The sub-district area is 30 square metres, repeats 3 times.The method that adopts medicament to irritate root is prevented and treated fusarium wilt, irritates root and after watermelon is transplanted back 7 days, begins, and whenever irritates root 1 time at a distance from 7 days, irritates root altogether 3 times, and every strain melon seedling pouring amount is 200 grams.The medicament of dilution directly waters in the watermelon root.Investigate the diseased plant rate of each all watermelons of sub-district after the dispenser of filling root in 80 days, calculate control efficiency then.
Visible from table 6, the effect of NUV234SE and NUV531SE control watermelon blight is respectively 80.9% and 80.2%, and difference is not remarkable between the two; And the effect of A01 concentrate and polyoxin control watermelon blight is respectively 60.5% and 65.6%, and difference is not remarkable yet between the two; The preventive effect of NUV234SE and NUV531SE obviously is superior to contrasting medicament A01 concentrate and polyoxin, and there are differences significantly.This result shows, adds the NUV234SE of UV absorbers and the effect that NUV531SE has obviously improved A01 concentrate (not adding UV absorbers) control watermelon blight.
Table 6NUV234SE and NUV531SE control watermelon blight result of the test
| Handle | NUV234SE | NUV531SE | The A01 concentrate | Polyoxin aqueous solution |
| Control efficiency % | 80.9a | 80.2a | 60.5b | 65.6b |
Annotate: data are the mean value of 3 repetitions in the table; Different letter representations behind the mean are through Duncan ' s multiple range test significant difference on the p=0.05 level.
List of references
1, Tanaka believes the man, and " mechanism of action of antibiotic " translation group is translated, the mechanism of action of antibiotic, Beijing Science Press, 1977
2, Lu Guoying, the progress of new food preservative natamycin, feed industry, 2005,26 (11): 28-31
3, Wang Guifang, Zhao Shaohua. the characteristic of natamycin and in Application in Food, Food Additives Used in China, 2006:2:144-151
4, Ceng Guangran, the application of farm antibiotics and development, Jilin agricultural science, 1989, (2): 28-36
5、Enshasy?H?A，Farid?M?A.J.Influence?of?inoculum?type?and?cultivation?conditions?onNatamycin?production?by?Streptomyces?natalensis，Basic?Microbiol.，2000，40(5-6)，333-342
6、Enshasy?H?A，Faird?M?A.J.，Optimization?of?the?cultivation?medium?for?natamycin?producitonby?Strepromyces?natalensis，Basic?Microbiol.，2000，40(3)，157-166)
7, Zheng Fenge etc., natamycin produces the Study on mutagenesis breeding of bacterium, food industry science and technology, 2008 (29), 8:159-160,172
8, Lu Suyun, biocontrol of plant disease is learned [M], and Beijing Agricultural University publishes, and 1993
Claims (9)
1. an anti-photodissociation bactericidal suspension emulsion is characterized in that, the active component of said anti-photodissociation bactericidal suspension emulsion is a natamycin, and the mass percent of its component is: natamycin 0.01%～6%; UV absorbers UV-234 or UV-5313%～30%; Toluene or xylol 3%～30%; Emulsifier 3%～15%; Antiprecipitant 0.5%～15%; Surplus is a water.
2. anti-photodissociation bactericidal suspension emulsion according to claim 1 is characterized in that, said suspension emulsion also comprises one or more in the following component of conventional using dosage, antifreeze, antifoaming agent and pH adjustment agent.
3. anti-photodissociation bactericidal suspension emulsion according to claim 1 is characterized in that, each component and the mass percent thereof of said suspension emulsion are: natamycin 0.05%～5%; UV absorbers 5%～20%; Toluene or xylol 5%～20%; Emulsifier 3%～10%; Antiprecipitant 1%～10%; Surplus is a water.
4. anti-photodissociation bactericidal suspension emulsion according to claim 1 is characterized in that, the source of said natamycin is streptomyces lydicus A01, brown yellow spore streptomycete, Natal streptomycete or antibiotic concentrate that streptomyces chatanoogensis produced.
5. anti-photodissociation bactericidal suspension emulsion according to claim 1 is characterized in that, said emulsifier is a farming breast 600
#, farming breast 601
#, farming breast 602
#With farming breast 500
#In one or more.
6. anti-photodissociation bactericidal suspension emulsion according to claim 1 is characterized in that said antiprecipitant is one or more in sodium carboxymethylcellulose, bentonite, xanthan gum and the Magnesiumaluminumsilicate.
7. according to the arbitrary described anti-photodissociation bactericidal suspension emulsion of claim 1-6, it is characterized in that the controlling object of said anti-photodissociation bactericidal suspension emulsion is a fungal disease.
8. the preparation method of the described anti-photodissociation bactericidal suspension emulsion of claim 1 is characterized in that, prepares suspension emulsion according to the above ratio, takes by weighing UV absorbers earlier; It is dissolved in toluene or the xylol, adds emulsifier after the heating for dissolving, stir; Obtain solution 1, and it is joined in the natamycin, the limit edged stirs; Until mixing, add antiprecipitant then and water stirs again, put into grinder be ground to 95% grain fineness less than 5 μ m till.
9. the method for using of the described anti-photodissociation bactericidal suspension emulsion of claim 1; It is characterized in that; Fall ill before or their early stage plant; Press the working concentration 10～100ppm of active component natamycin, suspension emulsion is converted water be mixed with dilution, adopt spray-on process or root-pouring method that it is sprayed on plant surface or imposes on plant root.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| CN102885056A (en) * | 2011-07-22 | 2013-01-23 | 湖北绿天地生物科技有限公司 | Natamycin preparation and preparation method thereof |
| CN102487950B (en) * | 2011-11-29 | 2013-08-21 | 北京市农林科学院 | Photolysis resistance sterilization microcapsule suspension and preparation method and application method of microcapsule |
| WO2014101237A1 (en) * | 2012-12-31 | 2014-07-03 | 深圳诺普信农化股份有限公司 | Photolysis-resistant pesticide and applications thereof |
| EP3003048A1 (en) * | 2013-05-31 | 2016-04-13 | DSM IP Assets B.V. | Microbial agriculture |
| CN103704271A (en) * | 2013-12-11 | 2014-04-09 | 上海交通大学 | Application of Streptomyces lydicus |
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| WO2007039572A1 (en) * | 2005-10-04 | 2007-04-12 | Dsm Ip Assets B.V. | Improved anti-fungal composition |
| CN101161083A (en) * | 2007-10-22 | 2008-04-16 | 山东农业大学 | Synergistic myprozine composition |
| CN101182485A (en) * | 2007-09-29 | 2008-05-21 | 北京市农林科学院 | Streptomyces lydicus producing natamycin and uses thereof |
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| CN101182485A (en) * | 2007-09-29 | 2008-05-21 | 北京市农林科学院 | Streptomyces lydicus producing natamycin and uses thereof |
| CN101161083A (en) * | 2007-10-22 | 2008-04-16 | 山东农业大学 | Synergistic myprozine composition |
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