CA2495398A1 - Antisense modulation of nav1.3 expression - Google Patents

Abstract

Antisense compounds, compositions, and methods are provided for modulating the expression of Type III sodium channel Nav1.3. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding Nav1.3. Methods of using these compounds for modulation of Nav1.3 expression and for treatment of diseases associated with expression of Nav1.3 are provided.

Description

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ANTISENSE MODULATION OF Navl.3 EXPRESSION
The present application claims priority under Title 35, United States Code, ~ 119 to United States Provisional application Serial No. 60/403,416, filed August 14, 2002, which is incorporated by reference in its entirety as if written herein.
FIELD OF THE INVENTION
[001] The present invention provides compositions and methods for modulating the expression of Type III sodium channel (Navl.3). In particular, this invention relates to antisense compounds, particularly oligonucleotides, specifically hybridizable with nucleic acids encoding Type III sodium channel (Navl.3). Such oligonucleotides have been shown to modulate the expression of Type III sodium channel (Navl.3).
BACKGROUND OF THE INVENTION
.. [002] Voltage-dependent sodium channels play a fundamental role in controlling neuronal membrane excitability. They are responsible for the upstroke of the action potential in neurons. Neuropathic pain is considered to be the result of injured and/or remodeled neurons, which exhibit an increase in excitability in both primary afferent fibers and synaptic contacts within the central pain processing pathway. Thus it is not surprising that three of the four drugs of choice used in the treatment of neuropathic pain syndromes are sodium channel blockers. These include carbamazepine, lamotrigine, and phenytoin. In addition, lidocaine, another sodium channel Mocker, was recently approved for treatment of certain types of neuropathic pain. However, although sodium channel blockers are currently the treatment of choice for neuropathic pain, they have been only moderately successful in treating these conditions. A primary factor in the weak efficacy of these compounds is their lack of selectivity and low affinity for the various sodium channels resulting in numerous side effects including emesis, dizziness, ataxia, and cardiotoxicity.
[003] Many recent efforts have been focused on defining and describing which sodium channels are actively involved in the development and maintenance of neuropathic pain. The alpha subunit is the pore forming subunit and to date, 11 alpha subunits have been identified including 7 neuronal, 2 glial, 1 muscular, 1 cardiac subunit. The alpha subunits can be divided into three classes based on their sensitivity to tetrodotoxin (TTX):
TTX sensitive (TTXs), TTX resistant (TTXr), and TTX insensitive (TTXi).
TTXs channels are sensitive to TTX in the nanomolar range, have a low threshold for activation and rapid inactivation kinetics. These channels include the skeletal muscle channel, two channels that are found primarily in peripheral neurons and all brain type channels described thus far.
[004] Recent reports have suggested that the type III sodium channel (Navl.3) may also be involved in neuropathic pain development and maintenance. This particular channel is found in brain, peripheral nerve and, neuroendocrine cells. Critically, it is absent from heart and many other peripheral organs. The mRNA for Navl .3 increases in small and large DRG
neurons following axotomy in animal models (Dib-Hajj S, et al.,

2 Proc.Natl.Acad.Sci. 1996; 93:14950-14954), suggesting its involvement in neuropathic pain. It has recently been demonstrated that peripheral nerve damage produced a large increase in the expression of a tetrodotoxin (TTX)-sensitive Navl .3 sodium channel (Boucher TJ, et al., Science 2000;
290:124-127) They also replicated past experiments from several labs establishing that the development of neuropathic pain is linked to increased spontaneous activity following nerve damage and that this ectopic activity is sensitive to TTX. Finally, the study from Wood's group also found that glial-derived neurotrophic factor (GDNF) prevented the induction of Navl.3, reduced the spontaneous activity of the injured nerve, thus preventing the resulting neuropathic pain. These studies implicated TTX-sensitive sodium channels, and in particular Navl.3, as having a role in the development and maintenance of neuropathic pain that follows nerve damage.
[005] Despite the variety of sodium channel inhibitors disclosed in the art, there still remains a need for therapeutic agents capable of effectively and specifically inhibiting the function of the Type III sodium channel (Navl.3). Furthermore, antisense technology could specifically target neonatal vs. adult forms of Navl .3. Exon 6 of the Navl .3 gene is alternatively spliced in humans (Lu C-M, Brown GB. J. Mol. Neu~osci.
1988; 10:67-70) and rodents (Gustafson TA, et al.. J. Biol. Claena. 1993;
268:18648-53). The two alternative exon 6 sequences are very similar, differing by only a single amino acid at the protein level, but they could be targeted individually by sequence-specific antisense oligonucleotides. This

3

4 PCT/US2003/025465 could be useful in the treatment of certain pediatric disorders, such as neonatal, infantile, or childhood epilepsies.
[006] Antisense technology is emerging as an effective means for reducing the expression of specific gene products and may therefore prove to be uniquely useful in a number of therapeutic, diagnostic, and research applications for the modulation of Navl.3 expression.
SUMMARY OF THE INVENTION
[007] The present invention is directed to antisense compounds, particularly oligonucleotides, which are targeted to a nucleic acid encoding Navl.3, and which modulate the expression of Navl.3. Pharmaceutical and other compositions comprising the antisense compounds of the invention are also provided. Further provided are methods of modulating the expression of Navl .3 in cells or tissues comprising contacting said cells or tissues with one or more of the antisense compounds or compositions of the invention.
Further provided are methods of treating an animal, particularly a human, suspected of having or being prone to a disease or condition associated with expression of Navl .3 by administering a therapeutically or prophylactically effective amount of one or more of the antisense compounds or compositions of the invention.
DETAILED DESCRIPTION OF THE INVENTION
[008] The present invention employs oligomeric antisense compounds, particularly oligonucleotides, for use in modulating the function of nucleic acid molecules encoding Navl.3, ultimately modulating the amount of Navl .3 produced. This is accomplished by providing antisense compounds, which specifically hybridize with one or more nucleic acids encoding Navl.3. As used herein, the terms "target nucleic acid" and "nucleic acid encoding Navl.3" encompass DNA encoding Navl.3, RNA (including pre-mRNA and mRNA) transcribed from such DNA, and also cDNA derived from such RNA. The specific hybridization of an oligomeric compound with its target nucleic acid interferes with the normal function of the nucleic acid. This modulation of function of a target nucleic acid by compounds, which specifically hybridize to it, is generally referred to as "antisense".
The functions of DNA to be interfered with include replication and transcription.
The functions of RNA to be interfered with include all vital functions such as, for example, translocation of the RNA to the site of protein translation, translation of protein from the RNA, splicing of the RNA to yield one or more mRNA species, and catalytic activity which may be engaged in or facilitated by the RNA. The overall effect of such interference with target nucleic acid function is modulation of the expression of Navl .3. In the context of the present invention, "modulation" means either an increase (stimulation) or a decrease (inhibition) in the expression of a gene. In the context of the present invention, inhibition is the preferred form of modulation, of gene expression and mRNA is a preferred target.
[009] It is preferred to target specific nucleic acids for antisense.
"Targeting" an antisense compound to a particular nucleic acid, in the context of this invention, is a multistep process. The process usually begins with the identification of a nucleic acid sequence whose function is to be modulated. This may be, for example, a cellular gene (or mRNA transcribed from the gene) whose expression is associated with a particular disorder or disease state, or a nucleic acid molecule from an infectious agent. In the present invention, the target is a nucleic acid molecule encoding Navl.3.
The targeting process also includes determination of a site or sites within this gene for the antisense interaction to occur such that the desired effect, e.g., detection or modulation of expression of the protein, will result.
Within the context of the present invention, a preferred intragenic site is the region encompassing the translation initiation or termination codon of the open reading frame (ORF) of the gene. Since, as is known in the art, the translation initiation codon is typically 5'-AUG (in transcribed mRNA
molecules; 5'-ATG in the corresponding DNA molecule), the translation initiation codon is also referred to as the "AUG codon," the "start codon" or the "AUG start codon". A minority of genes have a translation initiation codon having the RNA sequence 5'-GUG, 5'-UUG or 5'-CUG, and 5'-AUA, 5'-ACG and 5'-CUG have been shown to function in vivo. Thus, the terms "translation initiation codon" and "start codon" can encompass many codon sequences, even though the initiator amino acid in each instance is typically methionine (in eukaryotes) or fonnylmethionine (in prokaryotes).
It is also known in the art that eukaryotic and prokaryotic genes may have two or more alternative start codons, any one of which may be preferentially utilized for translation initiation in a particular cell type or tissue, or under a particular set of conditions. In the context of the invention, "start codon"
and "translation initiation codon" refer to the codon or codons that are used in vivo to initiate translation of an mRNA molecule transcribed from a gene encoding Navl .3, regardless of the sequences) of such colons.
[0010] It is also known in the art that a translation termination colon (or "stop colon") of a gene may have one of three sequences, i.e. 5'-UAA, 5'-UAG and 5'-UGA (the corresponding DNA sequences are 5'-TAA, 5'-TAG
and 5'-TGA, respectively). The terms "start colon region" and "translation initiation colon region "refer to a portion of such an mRNA or gene that encompasses from about 25 to about 50 contiguous nucleotides in either direction (i.e., 5' or 3') from a translation initiation colon. Similarly, the terms "stop colon region" and "translation termination colon region "refer to a portion of such an mRNA or gene that encompasses from about 25 to about 50 contiguous nucleotides in either direction (i.e., 5' or 3') from a translation termination colon.
[0011] The open reading frame (ORF) or "coding region," which is known in the art to refer to the region between the translation initiation colon and the translation termination colon, is also a region which may be targeted effectively. Other target regions include the 5' untranslated region (5'UTR), known in the art to refer to the portion of an mRNA in the 5' direction from the translation initiation colon, and thus including nucleotides between the 5' cap site and the txanslation initiation colon of an mRNA or corresponding nucleotides on the gene, and the 3' untranslated region (3'UTR), known in the art to refer to the portion of an mRNA in the 3' direction from the translation termination colon, and thus including nucleotides between the translation termination colon and 3' end of an mRNA or corresponding nucleotides on the gene. The 5' cap of an mRNA

comprises an N7-methylated guanosine residue joined to the 5'-most residue of the mRNA via a 5'-5' triphosphate linlcage. The 5' cap region of an mRNA is considered to include the 5' cap structure itself as well as the first SO nucleotides adjacent to the cap. The 5' cap region may also be a preferred target region.
[0012] Although some eulcaryotic mRNA transcripts are directly translated, many contain one or more regions, known as "introns," which are excised from a transcript before it is translated. The remaining (and therefore translated) regions are known as "exons" and are spliced together to form a continuous mRNA sequence. mRNA splice sites, i.e., intron-exon junctions, may also be preferred target regions, and are particularly useful in situations where aberrant splicing is implicated in disease, or where an overproduction of a particular mRNA splice product is implicated in disease. Aberrant fusion junctions due to rearrangements or deletions are also preferred targets. It has also been found that introns can also be effective, and therefore preferred, target regions for antisense compounds targeted, for example, to DNA or pre-mRNA.
[0013] Once one or more target sites have been identified, oligonucleotides axe chosen which are sufficiently complementary to the target, i.e., hybridize sufficiently well and with sufficient specificity, to give the desired effect.
[0014] In the context of this invention, "hybridization" means hydrogen bonding, which may be Watson-Crick, Hoogsteen or rever sed Hoogsteen hydrogen bonding, between complementary nucleoside or nucleotide bases.
For example, adenine and thymine are complementary nucleobases, which pair through the formation of hydrogen bonds. "Complementary," as used herein, refers to the capacity for precise pairing between two nucleotides.
For example, if a nucleotide at a certain position of an oligonucleotide is capable of hydrogen bonding with a nucleotide at the same position of a DNA or RNA molecule, then the oligonucleotide and the DNA or RNA are considered to be complementary to each other at that position. The oligonucleotide and the DNA or RNA are complementary to each other when a sufficient number of corresponding positions in each molecule are occupied by nucleotides which can hydrogen bond with each other. Thus, "specifically hybridizable" and "complementary" are terms which are used to indicate a sufficient degree of complementarity or precise pairing such that stable and specific binding occurs between the oligonucleotide and the DNA or RNA target. It is understood in the art that the sequence of an antisense compound need not be 100% complementary to that of its target nucleic acid to be specifically hybridizable. An antisense compound is specifically hybridizable when binding of the compound to the target DNA
or RNA molecule interferes with the normal function of the target DNA or RNA to cause a loss of utility, and there is a sufficient degree of complementarity to avoid non-specific binding of the antisense compound to non-target sequences under conditions in which specific binding is desired, i.e., under physiological conditions in the case of in vivo assays or therapeutic treatment, and in the case of in vitro assays, under conditions in which the assays are performed.
[0015] Antisense compounds are commonly used as research reagents and diagnostics. For example, antisense oligonucleotides, which are able to inhibit gene expression with exquisite specificity, are often used by those of ordinary skill to elucidate the function of particular genes. Antisense compounds are also used, for example, to distinguish between functions of various members of a biological pathway. Antisense modulation has, therefore, been harnessed for research use.
[0016] The specificity and sensitivity of antisense is also harnessed by those of skill in the art for therapeutic uses. Antisense oligonucleotides have been employed as therapeutic moieties in the treatment of disease states in animals and man. Antisense oligonucleotides have been safely and effectively administered to humans and numerous clinical trials are presently underway. It is thus established that oligonucleotides can be useful therapeutic modalities that can be configured to be useful in treatment regimes for treatment of cells, tissues and animals, especially humans. In the context of this invention, the term "oligonucleotide" refers to an oligomer or polymer of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA) or mimetics thereof. This term includes oligonucleotides composed of naturally occurring nucleobases, sugars and covalent internucleoside (backbone) linkages as well as oligonucleotides having non-naturally occurring portions which function similarly. Such modified or substituted oligonucleotides are often preferred over native forms because of desirable properties such as, for example, enhanced cellular uptake, enhanced affinity for nucleic acid target and increased stability in the presence of nucleases.
[0017] While antisense oligonucleotides are a preferred form of antisense compound, the present invention comprehends other oligomeric antisense compounds, including but not limited to oligonucleotide mimetics io comprises an N7-m such as are described below. The antisense compounds in accordance with this invention preferably comprise from about 8 to about 30 nucleobases {i.e. from about 8 to about 30 linced nucleo sides). Particularly preferred antisense compounds are antisense oligonucleotides, even more preferably those comprising from about 12 to about 25 nucleobases. As is known in the art, a nucleoside is a base-sugar combination. The base portion of the nucleoside is normally a heterocyclic base. The two most common classes of such heterocyclic bases are the purines and the pyrimidines. Nucleotides are nucleosides that further include a phosphate group covalently linked to the sugar portion of the nucleoside. For those nucleosides that include a pentofuranosyl sugar, the phosphate group can be linked to either the 2', 3' or 5' hydroxyl moiety of the sugar. In forming oligonucleotides, the phosphate groups covalently link adjacent nucleosides to one another to form a linear polymeric compound. In turn the respective ends of this linear polymeric structure can be further joined to form a circular structure, however, open linear structures are generally preferred. Within the oligonucleotide structure, the phosphate groups are commonly referred to as forming the internucleoside backbone of the oligonucleotide. The normal I
linkage or backbone of RNA and DNA is a 3' to 5' phosphodiester linkage.
[0018] Specific examples of preferred antisense compounds useful in this invention include oligonucleotides containing modified backbones or non-natural internucleoside linkages. As defined in this specification, oligonucleotides having modified backbones include those that retain a phosphorus atom in the backbone and those that do not have a phosphorus 2S atom in the backbone. For the purposes of this specification, and as sometimes referenced in the art, modif ed oligonucleotides that do not have a phosphorus atom in their intemucleoside backbone can also be considered to be oligonucleosides.
[0019) Preferred modified oligonucleotide backbones include, for example, phosphorothioates, chiral phosphorothioates, phosphorodithioates,~, phosphotriesters, aminoalkylphosphotriesters, methyl and other alkyl phosphonates including 3'alkylene phosphonates and chiral phosphonates, phosphinates, phosphoramidates including 3'-amino phosphoramidate and aminoalkylphosphoramidates, thionophosphoramidates, thionoalkylphosphonates, thionoalkylphosphotricstcrs, and boranophosphates having normal 3'-S' linkages, 2'-5' linked analogs of these, and those having inverted polarity wherein the adjacent pairs of nucleoside units are linked 3'-5' to S'-3' or 2'-S' to S'-2'. Various salts, mixed salts and free acid forms are also included.
IS [0020] Representative United States patents that teach the preparation of the above phosphorus-containing linkages include, but are not limited to, U.S. 3,687,808; 4,469,863; 4,4?6,301; 5,023,243; 5,177,196; 5,188,89?;

5,264,423; 5,276,019; 5,278,302; 5,286,717; 5,321,131; 5,399,676;
S,40S,939; S,4S3,496; S,4S5,233; 5,466,677; 5,476,925; 5,519,126;
5,536,821; 5,541,306; S,SS0,111; 5,563,253; 5,571,799; 5,587,361; and S,62S,050 .
[0021] Preferred modified oligonucleotide backbones that do not include a phosphorus atom therein have backbones that are formed by a~hort chain alkyl or cycloalkyl internucleoside linkages, mixed heteroatom and 2S alkyl or cycloalkyl intemucleoside linkages, or one or more short chain TZ

heteroatomic or heterocyclic internucleoside linkages. These include those having morpholino linkages (formed in part from the sugar portion of a nucleoside); siloxane backbones; sulfide, sulfoxide and sulfone backbones;
for~nacetyl and thiofoxmacetyl backbones; methylene formacetyl and thioformaeetyl backbanes; alkene containing backbones; sulfamate backbones; methyleneimino and methylenehydrazino backbones; sulfonate and sulfonamide backbones; amide backbones; and others having mixed N, O, S and CHz component parts.
[0022] Representative United States patents that teach the preparatioai of ~ 0 . the above oligonucleosides include, but are not limited to, U.S.
5,034,506;
5,166,315; 5,185,444; 5,214,134; 5,216,141; 5,235,033; 5,264,562;
5,264,564;5,405,938; 5,434,257; 5,466,677; 5,470,967; 5,489,677;
5,541,307; 5,561,225; 5,596,086; 5,602,240; 5,610,289; 5,602,240;
5,608,046; 5,610,289; 5,618,704; 5,623,070; 5,663,312; 5,633,360;
5,677,437; and 5,677,439 .
[0023j In other preferred oligonucleotide mimetics, both the sugar and the internucleoside linkage, i.e., the backbone, of the nucleotide units are replaced with novel groups. The base units are maintained for hybridization with an appropriate nucleic acid target compound. One such oligomerie compound, an oligonucleotide mimetic that has been shown to have excellent hybridization properties, is referred to as a peptide nucleic acid (PNA). In PNA compounds, the sugar backbone of an oligonucleotide is replaced with an amide containing backbone, in particular an aminoethylglycine backbone. The nucleobases are retained and are bound directly or indirectly to aza nitrogen atoms of the amide portion of the backbone. Representative United States patents that teach the preparation of PNA compounds include, but are not limited to, U.S. 5,539,082; 5,714,331;
and 5,719,262. Further teaching ofPNA compounds can be found in Nielsen et al., Science, 1991, 254, 1497-1500.
[0024] Most preferred embodiments of the invention are oligonucleotides with phosphorothioate backbones and oligonucleosides with heteroatom backbones, and in particular -CH2 NH-O-CHZ-, -CHI-N
(CH3) -O-CHZ- [Irnown as a methylene (methylimino) or MMI backbone] , -CH2-O-N (CH3) -CHZ-, -CH2N(CH3)-N(CH~)-CHI- and -O-N(CH3)-CHZ-CHZ- jwherein the native phosphodiester backbone is represented as -O-P-O-CHZ-] of the above referenced U.S. patent 5,489,677, and the amide backbones of the above referenced U.S. patent 5,602,240. Also preferred are oligonucleotides having rnorpholino backbone structures of the above-referenced U.S. patent 5,034,506.
[0025] Modified oligonucleotides may also contain one or more substituted sugar moieties. Preferred oligonucleotides comprise one of the following at the 2' position: OH; F; O-, S-, or N-alkyl; O-, S-, or N-alkenyl;
O-, S- or N-aikynyl; or O-alkyl-O-alkyl, wherein the alkyl, alkenyl and alkynyl may be substituted or unsubstituted Cl to Clo alkyl or C2 to Coo allcenyl and alkynyl. Particularly preferred are O[(CH2~0]mCH3, O(CH2~",OCH3, O(CHZ)"NHz, O(CHZh,CH3, O(CHz)"ONH2, and O(CHZ"ON[(CH2)"CH3)]Z where n and m are from 1 to about 10. Other preferred oligonucleotides comprise one of the following at the 2' position:

CI to Coo, ( lower alkyl, substituted lower alkyl, alkaryl, aralkyl, O-allcaryl or O-aralkyl, SH, SCH3, OCN, Cl, Br, CN, CF3, OCF3, SOCH3, SOZCH3, ONOz, N02, N3, NH2, heterocycloalkyl, heterocycloalkaryl, aminoalkylamino, polyallcylamino, substituted silyl, an RNA cleaving group, a reporter group, an intercalator, a group for improving the pharmacokinetic properties of an oligonucleotide, or a group for improving the pharmacodynamic properties of an oligonucleotide, and other substituents having similar properties. A preferred modification includes 2' -methoxyethoxy (2' -O-CH2CH20CH3, also known as 2'-O- (2-methoxyethyl) or 2'-MOE) (Martin et al., Helv. Chim. Acta, 1995, 78, 486-504) i.e., an alkoxyalkoxy group. A further preferred modification includes 2'-dimethylaminooxyethoxy, i.e., a O(CH2)20N(CH3)2 group, also known as 2'-DMAOE, as described in examples herein below, and 2'-dimethylaminoethoxyethoxy (also known in the art as 2'-O-dimethylaminoethoxyethyl or 2'-DMAEOE), i.e., 2'-O-CHZ-O-CH2-N
(CH2)z, also described in examples herein below.
[0026] Other preferred modifications include 2'-methoxy (2'-O CH3), 2'-aminopropoxy (2'-O CH2 CHZ CHZNH2) and 2'-fluoro (2'-F). Similar modifications may also be made at other positions on the oligonucleotide, particularly the 3' position of the sugar on the 3' terminal nucleotide or in 2'-5' linked oligonucleotides and the 5' position of 5' terminal nucleotide.
Oligonucleotides may also have sugar rnimetics such as cyclobutyl moieties in place of the pentofuranosyl sugar. Representative United States patents that teach the preparation of such modified sugar structures include, but are not limited to, U.S. 4,981,957; 5,118,800; 5,319,080; 5,359,044; 5,393,878;
Is 5,446,137; 5,466,786; 5,514,785; 5,519,134; 5,567,811; 5,576,427;
5,591,722; 5,597,909; 5,610,300; 5,627,053; 5,639,873; 5,646,265;
5,658,873; 5,670,633; and 5,700,920 .
[002?] Oligonucleotides may also include nucleobase (often referred tp in the art simply as "base") modifications or substitutions. As used herein, "unmodified" or "natural" nucleobases include the purine bases adenine (A) and guanine (G), and the pyrimidine bases thymine ('I~, cytosine (C) and uracil (f~. Modified nucleobases include other synthetic and natural nucleobases such as 5-methylcytosine (5-me-C), 5-hydroxymethyl cytosine, xanthine, hypoxanthine, 2-aminoadenine, 6-methyl and other alkyl derivatives of adenine and guanine, 2 propyl and other alkyl derivatives of adenine and guanine, 2-thiouracil, 2-thiothymine and 2-thiocytosine, 5-halouracil and cytosine, 5-propynyl uracil and cytosine, 6-azo uracil, cytosine and thymine, S-uracil (pseudouraeil), 4-thiouracil, 8-halo, 3-amino, 8-ihiol, 8-thioallcyl, 8 hydroxyl and other 8-substituted adenines and guanines, 5-halo particularly 5 bromo, 5-trifluoromethyl and other 5-substituted uracils and cytosines, 7-methylquanine and 7 methyladenino, 8-azaguanine and 8-azaadenine, 7-deazaguanine and 7-deazaaderiine and 3-deazaguanine and 3-deazaadenine. Further nucleobases include those disclosed in Unitcd States Patent No. 3,687,808, those disclosed in The Concise Encyclopedia Of Polymer Science And Engineering, pages 858-859, Kroschwitz, J.L, ed. John Wilcy & Sons, 1990, those disclosed by Englisch et al., Angewandte Chemie, International Edition,1991, 30, 613, and those disclosed by Sanghvi, Y.S:, Chapter 15, Antisense Research and Applications, pages 289-302, Crooke, S.T. and Lebleu, B. ed., CRC Press, 1993. Certain of these nucleobases are particularly useful for increasing the binding affinity of the oligomeric compounds of the invention. These include S-substituted pyrimidines, 6-azapyrimidines and N-2, N-6 and O-6 S substituted purines, including 2-aminopropyladenine, S propynyluracil and S-propyriylcytosine. 5-methylcytosine substitutions have bees shown to increase nucleic acid duplex atabdity by 0.6-1.2°C (Sanghvi, Y.S., Crooke, S.T. and Ixbleu, B., eds, Antisense Research and Applications, CRC Press, Boca Raton, 1993, pp. 276.-278) and are presently preferred base substitutions, even more particularly when combined with 2'-O-methoxyethyl sugar modifications.
[0028] Representative United States patents that teach the preparation of certain of the above noted modified nucleobases as well as other modi$ed nucleobases include, but are not limited to, the above noted U.S. 3,687,808, 1S as well as U.S. 4,845,205; 5,134,302; 5,134,066; 5,175,273; 5,367,066;
5,432,272; 5,457,187; 5,459,255; 5,484908; 5,502,177; S,S25,711;
S,SS2,S40; 5;587,469; 5,594,121, S,S96,091; 5,614,617; S,7S0,692; and 5,681,941.
[0029j Another modificaxion of the oligonucleotides of the invention involves chemically linking to the oligonucleotide one or more moieties or conjugates, which enhance the activity, cellular distribution or cellular uptake of the oligonucleotide. Such moieties include but are not limited to lipid moieties such as a cholesterol moiety (Ixtsinger et al., Proc. Natl.
Aced. Sci. USA,1989, 86, 6553-6556), cholic acid (Manoharan et al., 2S Bioorg: Med Chem. Let., 1994, 4,1053-1060), a thioether, e.g., hexyl-S-tritylthiol (Manoharan et al., Ann. N Y. Acad. Sci., 1992, 660, 306-309;
Manoharan et al., Bioosg. Med. Claena. Let., 1993, 3, 2765-2770), a thiocholesterol (Oberhauser et al., Nucl. Acids Res., 1992, 20, 533-538), an aliphatic chain, e.g., dodecandiol or undecyl residues (Saison-Behmoaras et al., EMBO J., 1991, 10, 1111-1118; Kabanov et al., FEBS Lett., 1990, 259, 327-330; Svinarchuk et al., Bioclainaie, 1993, 75, 49-54), a phospholipid, e.g., di-hexadecyl-rac-glycerol or triethylammonium 1,2-di-O-hexadecyl-rac-glycero-3-H-phosphonate (Manoharan et al., Tetrahedron Lett., 1995, 36, 3651-3654; Shea et al., Nucl. Acids Res., 1990, 18, 3777-3783), a polyamine or a polyethylene glycol chain (Mancharan et al., Nucleosides ~
Nucleotides, 1995, 14, 969-973), or adamantane acetic acid (Manoharan et al., Tetrahedron Lett., 1995, 36, 365'-3654), a palmityl moiety (Mishra et al., Biochim. Biophys. Acta, 1995, 1264, 229-237), or an octadecylamine or hexylamino-carbonyl-oxycholesterol moiety (Crooke et al., J. Plaarmacol.
Exp. Tlaer., 1996, 277, 923-937).
[0030] Representative United States patents that teach the preparation of such oligonucleotide conjugates include, but are not limited to, U.S.
4,828,979; 4,948,882; 5,218,105; 5,525,465; 5,541,313; 5,545,730;
5,552,538; 5,578,717, 5,580,731; 5,580,731; 5,591,584; 5,109,124;
5,118,802; 5,138,045; 5,414,077; 5,486,603; 5,512,439; 5,578,718;
5,608,046; 4,587,044; 4,605,735; 4,667,025; 4,762,779; 4,789,737;
4,824,941; 4,835,263; 4,876,335; 4,904,582; 4,958,013; 5,082,830;
5,112,963; 5,214,136; 5,082,830; 5,112,963; 5,214,136; 5,245,022;
5,254,469; 5,258,506; 5,262,536; 5,272,250; 5,292,873; 5,317,098;
5,371,241, 5,391,723; 5,416,203, 5,451,463; 5,510,475; 5,512,667;

Applications, pages 289 5106'7-55 S,SI4,?8S; S,S6S,SS2; S,S67,810; 5,574,142; S,S8S,481; S,S87,371;
S,S9S,726; 5,597,696; S,S99,923; 5,599,928 and S,b88,941.
[0031) It is not necessary for all positions in a given compound to be S uniformly modified, and in fact more than one of the aforementioned , modifications may be incorporated in a single compound or even at a single nucleoside withia an oligonucleotide. The present invention also includes antise~nse compounds, which are chimerio compounds. "Chimerie" antisense compounds ox "chimeras," in the context of this invention, are amisense compounds, particularly oligonucleotides, which contain two or more chemically distinct regions, each made up of at least one monomer unit, i.e., a nucleotide in the case of an oligonucleotide compound. These oligonucleotides typically contain at least one region wherein the oligonucleotide is modified so as to confer upon the oligonucleotide increased resistrince to nuclease degradation, increased cellular uptake, and/or increased binding affinity for the target nucleic acid. An additional region of the oligonucleotide may serve as a substrate for enzymes capable of cleaving RNA:DNA or RNA:RNA hybrids. By way of example, RNase H is a cellular endonuclease, which cleaves the RNA strand of RNA:DNA
duplex. Activation of RNase H, therefore, results in cleavage of the RNA, target, thereby greatly enhancing the efficiency of oligonucleotide inlu'bitioa of gene expression. Consequently, comparable results can often be obtained with shorter oligonucleotides when chimeric oligonucleotides are used, compared to phosphorothioate deoxyoligonucleotides hybridizing to the 2S same target region. Cleavage of the RNA target can be routinely detected by 5106'7-55 gel electrophoresis and, if necessary, associated nucleic acid hybridization techniques known in the art.
[0032] Chimeric antisense compounds of the invention may be formed as composite structures of two or more oligonucleotides, modified oligonucleotides, oligonucleosides and/or oligonucleotide mimetics as descn'lxd above. Such compounds have also been refernd to in the art as hybrids or gapmers. Representative United States pates that teach the preparation of such hybrid structures include, but are not limited to, U.S.
5,013,830; 5,149,797; 5,220,007; 5,256,775; 5,366,878; 5,403,711;
5,491,133; 5,565,350; 5,623,065; 5,652,355; 5,652,356; and 5,700,922 .
[0033] The antisense compounds used in accordance with this invention may be conveniently, and routinely made through the well-known technique of solid phase synthesis. Equipment for such synthesis is sold by several vendors including, for example, Applied Biosystems (Foster City, CA). Any other means for such synthesis known in the art may additionally or alternatively be employed. It is well known to use similar techniques to prepare oligonucleotides such as the phosphorothioates and alkylated derivatives.
[0034] The antisense compounds of the invention are synthesized in vitro and do not include antisense compositions of biological origin, or genetic vector constructs designed to direct the in vivo synthesis of antisense molecules. The compounds of the invention may also be admixed, encapsulated, conjugated or otherwise associated with other molecules, molecule structures or mixtures of compounds, as for example, liposomes, receptor targeted molecules, oral, rectal, topical or other formulations, for assisting in uptake, distn'bution and/or absorption. Representative United States patents that teach the preparation of such uptake, distribution and/or absorption assisting formulations include, but are not limited to, U.S.
5,108,921; 5,354,844; 5,416,016; 5,459,127; 5,521,291; 5,543,158;
5,547,932; 5,583,020; 5,591,721; 4,426,330; 4,534,899; 5,013,556;
5,108,921; 5,213,804; 5,227,170; 5,264,221; 5,356;633; 5,395,619;
5,416,016; 5,417,978; 5,462,854; 5,469,854; 5,512,295; 5,527,528;
5,534;259; 5,543,152; 5,556,948; 5,580,575; and 5,595,756 .
[0035] The antisense compounds of the invention encompass any pharmaceutically acceptable salts, esters, or salts of such esters, or any other compound which, upon administration to an animal including a human, is capable of providing (directly or indirectly) the biologically active metabolite or residue thereof. Accordingly, for example, the disclosure is also drawn to prodrugs and pharmaceutically acceptable salts of the compounds of the invention, pharmaceutically acceptable salts of such prodrugs, and other bioequivalents.
[0036] The term "prodrug" indicates a therapeutic agent that is prepared in an inactive form that is converted to an active form (i.e., drug) within the body or cells thereof by the action of endogenous enzymes or other chemicals and/or conditions. In particular, prodrug versions of the oligonucleotides of the invention are prepared as SATE [(S-acetyl-2-thioethyl) phosphate] derivatives according to the methods disclosed in WO
2~

93/24510 to Gosselin et al., published December 9, 1993 or in WO
94/26764 to Imbach et al.
[0037] The term "pharmaceutically acceptable salts" refers to physiologically and pharmaceutically acceptable salts of the compounds of the invention: i.e., salts that retain the desired biological activity of the parent compound and do not impart undesired toxicological effects thereto.
[0038] Pharmaceutically acceptable base addition salts are formed with metals or amines, such as alkali and alkaline earth metals or organic amines.
Examples of metals used as cations are sodium, potassium, magnesium, calcium, and the like. Examples of suitable amines are N, N'-dibenzylethylenediamine, chloroprocaine, choline, diethanolamine, dicyclohexylamine, ethylenediamine, N-methylglucamine, and procaine (see, for example, Berge et al., "Pharmaceutical Salts," J. of Phaf°jna Sci., 1977, 66, 119). The base addition salts of said acidic compounds are prepared by contacting the free acid form with a sufficient amount of the desired base to produce the salt in the conventional manner. The free acid form may be regenerated by contacting the salt form with an acid and isolating the free acid in the conventional manner. The free acid forms differ from their respective salt forms somewhat in certain physical properties such as solubility in polar solvents, but otherwise the salts are equivalent to their respective free acid for purposes of the present invention. As used herein, a "pharmaceutical addition salt" includes a pharmaceutically acceptable salt of an acid form of one of the components of the compositions of the invention. These include organic or inorganic acid salts 2S of the amines. Preferred acid salts are the hydrochlorides, acetates, salicylates, nitrates and phosphates. Other suitable pharmaceutically acceptable salts are well known to those skilled in the art and include basic salts of a variety of inorganic and organic acids, such as, for example, with inorganic acids, such as fox example hydrochloric acid, hydrobromic acid, sulfuric acid or phosphoric acid; with organic carboxylic, sulfonic, sulfo or phospho acids or N-substituted sulfamic acids, for example acetic acid, propionic acid, glycolic acid, succinic acid, malefic acid, hydroxymaleic acid, methylmaleic acid, fumaric acid, malic acid, tartaric acid, lactic acid, oxalic acid, gluconic acid, glucaric acid, glucuronic acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, salicylic acid, 4-aminosalicylic acid, 2-phenoxybenzoic acid, 2-acetoxybenzoic acid, embonic acid, nicotinic acid or isonicotinic acid; and with amino acids, such as the 20 alpha-amino acids involved in the synthesis of proteins in nature, for example glutamic acid or aspartic acid, and also with phenylacetic acid, methanesulfonic acid, ethanesulfonic acid, 2-hydroxyethanesulfonic acid, ethane-1,2-disulfonic acid, benzenesulfonic acid, 4-methylbenzenesulfaic acid, naphthalene-2-sulfonic acid, naphthalene-1,5-disulfonic acid, 2- or 3-phosphoglycerate, glucose-6-phosphate, N-cyclohexylsulfamic acid (with the formation of cyclamates), or with other acid organic compounds, such as ascorbic acid.
Pharmaceutically acceptable salts of compounds may also be prepared with a pharmaceutically acceptable cation. Suitable pharmaceutically acceptable canons are well known to those skilled in the art and include alkaline, alkaline earth, ammonium and quaternary ammonium cations. Carbonates or hydrogen carbonates are also possible.

[0039] For oligonucleotides, preferred examples of pharmaceutically acceptable salts include but are not limited to (a) salts formed with cations such as sodium, potassium, ammonium, magnesium, calcium, polyamines such as spennine and spermidine, etc.; (b) acid addition salts formed with inorganic acids, for example hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid and the like; (c) salts formed with organic acids such as, for example, acetic acid, oxalic acid, tartaric acid, succinic acid, malefic acid, fumaric acid, gluconic acid, citric acid, malic acid, ascorbic acid, benzoic acid, tannic acid, palmitic acid, alginic acid, polyglutamic acid, naphthalenesulfonic acid, methanesulfonic acid, p-toluenesulfonic acid, naphthalenedisulfonie acid, polygalacturonic acid, and the like; and (d) salts formed from elemental anions such as chlorine, bromine, and iodine.
[0040] The antisense compounds of the present invention can be utilized for diagnostics, therapeutics, prophylaxis, and as research reagents and kits.
For therapeutics, an animal, preferably a human, suspected of having a disease or disorder, which can be treated by modulating the expression of Navl .3, is treated by administering antisense compounds in accordance with this invention. The compounds of the invention can be utilized in pharmaceutical compositions by adding an effective amount of an antisense compound to a suitable pharmaceutically acceptable diluent or carrier. Use of the antisense compounds and methods of the invention may also be useful prophylactically, e.g., to prevent or delay infection, inflammation or tumor formation, fox example.

5106'7-55 [0041] The antiscnse compounds of the invention are useful for research and diagnostics; because these compounds hybridize to nucleic acids encoding Navl.3, enabling sandwich and other assays to easily be constructed to exploit this fact. Hybi~dization of the antisense oligonucleotides of the invention with a nucleic acid encodiag Navl.3 can ;
be detected by means known in the atrt. Such means may include conjugation of an enzyme to the oligonucleotide, radiolalxlling of the oligonucleotidc or any other suitable detection weans. Kits using such detection means for detecting the level of Navl .3 in a sample may also be prepared.
[0042] The present invention also includes pharmaceutical compositions and formulations, which include the antisense compounds of the invention.
The pharmaceutical compositions of the present invention may be administered in a number of ways depending upon whether local or 1 ~ systemic treatment is desired and upon the area to be treated Administration may be topical (including ophthalmic and to mucous membranes including vaginal and rectal delivery), pulmonary, e.g., by inhalation or insufflation of powders or aerosols, including by nebulizer; intratracheal, intranasal, epiderraal and transdermal), oral or parenteral. Parenteral administration includes intravenous, intraarterial, subcutaneous, intraperitoneal or intramuscular injection or infusion; or intracranial, e.g., intrathecal or intraventricular, administration. Oligonucleotides with at least one 2'-O-methoxyethyl modification are believed to be particularly useful for oral administration. The present invention also includes commercial packages comprising compounds as described herein together with instructions for use.

[0043] Intraventricular drug administration, for the direct delivery of drug to the brain of a patient, may be desired for the treatment of patients with diseases or conditions afflicting the brain. To effect this mode of oligonucleotide administration, a silicon catheter is surgically introduced into a ventricle of the brain of a human patient, and is connected to a subcutaneous infusion pump (SynchroMed 0 and IsoMee infusion systems, Medtronic Inc., Minneapolis, Minn.) that has been surgically implanted in the abdominal region (Zinnu et al., Cancef°
Reseaf~ch, 1984, 44, 1698. The pump is used to inject the oligonucleotides and allows precise dosage adjustments and variation in dosage schedules with the aid of an external programming device. The reservoir capacity of the pump is 18-20 mL
and infusion rates may range from 0.1 mL/h to 1 mL/h. Depending on the frequency of administration, ranging from daily to monthly, and the dose of drug to he administered, ranging from 0.01 micro g to I00 g per kg of body weight, the pump reservoir may be refilled at 3 -10 week intervals. Refilling of the pump is accomplished by percutaneous puncture of the self sealing septum of the pump.
[0044] Intrathecal Epidural, Subdural, drug administration, for the introduction of a drug into the spinal column of a patient may be desired for the treatment of patients with diseases of the central nervous system. To effect this route of oligonucleotides administration, a silicon catheter is surgically implanted subarachnoid spinal interspace of a human patient for example implantation into the L3-4 lumbar cord would target the legs), and is connected to a subcutaneous infusion pump which has been surgically implanted in the upper abdominal region (Leer and Hatton, The Annals of Plaas°macotlae~°apy, 1993, 27, 912; Ettinger et al., 1978, Cancef°, 41, 1270, 1978; Yaida et al., Regul. Pept., 1995, 59, 193). The pump is used to inject the oligonucleotides and allows precise dosage adjustments and variations in dose schedules with the aid of an external programming device.
The reservoir capacity of the pump is 1 S-20 mL, and infusion rates may vary from 0. 1 mL/h to 1 mL& Depending on the frequency of drug administration, ranging from daily to monthly, and dosage of drug to be administered, ranging from 0.01 micro g to 100 g per kg of body weight, the pump reservoir may be refilled at 3 -10 week intervals. Refilling of the pump is accomplished by a single percutaneous puncture to the self sealing septum of the pump. The distribution, stability and pharmacokinetics of oligonucleotides within the central nervous system may be followed according to known methods. Subdural administration is also envisioned other by a pump mechanism or direct injection.
[0045] To effect delivery of oligonucleotides to areas other than the brain or spinal column via this method, the silicon catheter is configured to connect the subcutaneous infusion pump to, e.g., the hepatic artery, for delivery to the liver.
Infusion pumps may also be used to effect systemic delivery of oligonucleotides 1 S (Ewel et al., Ca~eer° Research, 1992, 52, 3005; Rubenstein et al., J. Surg. Oncol., 1996, 62, 194).
[0046] Pharmaceutical compositions and formulations for topical administration may include transdermal patches, ointments, lotions, creams, gels, drops, suppositories, sprays, liquids and powders. Conventional pharmaceutical carriers, aqueous, powder or oily bases, thickeners and the like may be necessary or desirable. Coated condoms, gloves and the like may also be useful.
[0047] Compositions and formulations for oral administration include powders or granules, suspensions or solutions in water or non-aqueous media, capsules, sachets or tablets. Thickeners, flavoring agents, diluents, emulsifiers, dispersing aids or binders may be desirable.
[0048] Compositions and formulations for parenteral, intrathecal or intraventxicular administration may include sterile aqueous solutions, which may also contain buffers, diluents and other suitable additives such as, but not limited to, penetration enhancers, carrier compounds and other pharmaceutically acceptable carriers or excipients.
[0049] Pharmaceutical compositions of the present invention include, but are not limited to, solutions, emulsions, and liposome-containing formulations. These compositions may be generated from a variety of components that include, but are not limited to, preformed liquids, self emulsifying solids and self emulsifying semisolids.
[0050] The pharmaceutical formulations of the present invention, which may conveniently be presented in unit dosage form, may be prepared according to conventional techniques well known in the pharmaceutical industry. Such techniques include the step of bringing into association the active ingredients with the pharmaceutical carri.er(s) or excipient(s). In general the formulations are prepared by uniformly and intimately bringing into association the active ingredients with liquid carriers or finely divided solid carriers or both, and then, if necessary, shaping the product.
[0051] The compositions of the present invention may be formulated into any of many possible dosage forms such as, but not limited to, tablets, capsules, liquid syrups, soft gels, suppositories, and enemas. The compositions of the present invention may also be formulated as 2S suspensions in aqueous, non-aqueous or mixed media. Aqueous suspensions may further contain substances, which increase the viscosity of the suspension including, for example, sodium carboxymethylcellulose, sorbitol and/or dextran. The suspension may also contain stabilizers.
[0052] In one embodiment of the present invention the pharmaceutical compositions may be formulated and used as foams. Pharmaceutical foams include formulations such as, but not limited to, emulsions, microemulsions, creams, jellies and liposomes. While basically similar in nature these formulations vary in the components and the consistency of the final product. The preparation of such compositions and formulations is generally known to those skilled in the pharmaceutical and formulation arts and may be applied to the formulation of the compositions of the present invention.
Emulsions [0053] The compositions of the present invention may be prepared and formulated as emulsions. Emulsions are typically heterogenous systems of one liquid dispersed in another in the form of droplets usually exceeding 0.1 ~,m in diameter. (Idson, in Pharmaceutical Dosage Forms, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New York, N.Y., volume 1, p. 199; Rosoff, in Pharn2aceutical Dosage Forms, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New York, N.Y., Volume 1, p. 245; Block in Pharmaceutical Dosage For°rns, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New York, N.Y., volume 2, p. 335; Higuchi et al., in Rernington's Pharmacezatical Sciences, Mack Publishing Co., Easton, PA, 1985, p. 301). Emulsions.are often biphasic systems comprising of two immiscible liquid phases intimately mixed and dispersed with each other. In general, emulsions may be either water-in-oil (w/o) or of the oil-in-water (o/w) variety. When an aqueous phase is finely divided into and dispersed as minute droplets into a bulk oily phase the resulting composition is called a water-in-oil (w/o) emulsion.
Alternatively, when an oily phase is finely divided into and dispersed as S minute droplets into a bulk aqueous phase the resulting composition is called an oil-in-water (o/w) emulsion. Emulsions may contain additional components in addition to the dispersed phases and the active drug, which may be present as a solution in either the aqueous phase, oily phase or itself as a separate phase. Pharmaceutical excipients such as emulsifiers, stabilizers, dyes, and anti-oxidants may also be present in emulsions as needed. Pharmaceutical emulsions may also be multiple emulsions that are comprised of more than two phases such as, for example, in the case of oil-in-water-in-oil (o/w/o) and water-in-oil-in-water (w/o/w) emulsions. Such complex formulations often provide certain advantages that simple binary 1 S emulsions do not. Multiple emulsions in which individual oil droplets of an o/w emulsion enclose small water droplets constitute a w/o/w emulsion.
Likewise a system of oil droplets enclosed in globules of water stabilized in an oily continuous provides an o/w/o emulsion.
[0054] Emulsions are characterized by little or no thermodynamic stability. Often, the dispersed or discontinuous phase of the emulsion is well dispersed into the external or continuous phase and maintained in this form through the means of emulsifiers or the viscosity of the formulation. Either of the phases of the emulsion may be a semisolid or a solid, as is the case of emulsion-style ointment bases and creams. Other means of stabilizing 2S emulsions entail the use of emulsifiers that may be incorporated into either phase of the emulsion. Emulsifiers may broadly be classified into four categories: synthetic surfactants, naturally occurring emulsifiers, absorption bases, and finely dispersed solids (Idson, in Pharmaceutical Dosage Fof~ms, Lieberman, Rieger and Baz~lcer (Eds.), 1988, Marcel Dekker, Inc., New Yorlc, N.Y., volume 1, p. 199).
[0055] Synthetic surfactants, also known as surface active agents, have found wide applicability in the formulation of emulsions and have been reviewed in the literature (Rieger, in Pharmaceutical Dosage Forms, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New York, N.Y., volume 1, p. 285; Idson, in Pharmaceutical Dosage Forrns, Lieberman, Rieger and Banker (Eds.), Marcel Dekker, Inc., New York, N.Y., 1988, volume 1, p. 199). Surfactants are typically amphiphilic and comprise a hydrophilic and a hydrophobic portion. The ratio of the hydrophilic to the hydrophobic nature of the surfactant has been termed the hydrophile/lipophile balance (HLB) and is a valuable tool in categorizing and selecting surfactants in the preparation of formulations. Surfactants may be classified into different classes based on the nature of the hydrophilic group: nonionic, anionic, cationic and amphoteric (Rieger, in Pharmaceutical Dosage Fof°ms, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New York, N.Y., volume l, p. 285).
[0056] Naturally occurring emulsifiers used in emulsion formulations include lanolin, beeswax, phosphatides, lecithin and acacia. Absorption bases possess hydrophilic properties such that they can soak.up water to form w/o emulsions yet retain their semisolid consistencies, such as 2S anhydrous lanolin and hydrophilic petrolatum. Finely divided solids have also been used as good emulsifiers especially in combination with surfactants and in viscous preparations., These include polar inorganic solids, such as heavy metal hydroxides, nanswelling clays such as bentonite, attapulgite, hectorite, kaolin, montmorillonite, colloidal aluminum silicate and colloidal magnesium aluminum silicate, pigments and nonpolar solids such as carbon or glyceryl tristearate.
[0057) A large variety of non-emulsifying materials are also included in emulsion formulations and contribute to the properties of emulsions. These include fats, oils, waxes, fatty acids, fatty alcohols, fatty esters, humectants, hydrophilic colloids, preservatives, and antioxidants (Block, in Pharmaceutical Dosage Forms, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New York, N.Y., volume 1, p. 335; Idson, in Pharmaceutical Dosage Forms, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New York, N.Y., volume 1, p. 199).
[005] Hydrophilic colloids or hydrocolloids include naturally occurring gums and synthetic polymers such as polysaccharides (for example, acacia, agar, alginic acid, carrageenan, guar gum, karaya gum, and tragacanth), cellulose derivatives (for example, carboxymethylcellulose and carboxypropylcellulose), and synthetic polymers (for example, carbomers, cellulose ethers, and carboxyvinyl polymers). These disperse or swell in water to form colloidal solutions that stabilize emulsions by forming strong interfacial films around the dispersed phase droplets and by increasing the viscosity of the external phase.
[0059] Since emulsions often contain a number of ingredients such as carbohydrates, proteins, sterols and phosphatides that may readily support the growth of microbes, these formulations often incorporate preservatives.
Commonly used preservatives included in emulsion formulations include methyl paraben, propyl paraben, quaternary ammonium salts, benzalkonium chloride, esters of p-hydroxybenzoic acid, and boric acid. Antioxidants axe also commonly added to emulsion formulations to prevent deterioration of the formulation. Antioxidants used may be free radical scavengers such as tocopherols, alkyl gallates, butylated hydroxyanisole, butylated hydroxytoluene, or reducing agents such as ascorbic acid and sodium metabisulfite, and antioxidant synergists such as citric acid, tartaric acid, and lecithin.
[0060] The application of emulsion forinulations via dennatological, oral, and parenteral routes and methods for their manufacture have been reviewed in the literature (Idson, in Plaanmaceutical Dosage Foy~rns, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New York, N.Y., volume 1, p. 199). Emulsion formulations for oral delivery have been very widely used because of reasons of ease of formulation, efficacy from an absorption and bioavailability standpoint. (Rosoff, in Pharmaceutical Dosage Forms, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New York, N.Y., volume 1, p. 245; Idson, in Pharmaceutical Dosage Forms, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New York, N.Y., volume 1, p. 199). Mineral-oil base laxatives, oil-soluble vitamins and high fat nutritive preparations are among the materials that have commonly been administered. orally as o/w emulsions.

[0061] In one embodiment of the present invention, the compositions of oligonucleotides and nucleic acids are formulated as microemulsions. A
microemulsion may be defined as a system of water, oil and amphiphile, which is a single optically isotropic, and thermodynamically stable liquid solution (Rosoff, in Pharmaceutical Dosage Fog°nZS, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New York, N.Y., volume l, p.
245). Typically microemulsions are systems that are prepared by first dispersing an oil in an aqueous surfactant solution and then adding a sufficient amount of a fourth component, generally an intermediate chain-length alcohol to form a transparent system. Therefore, microemulsions have also been described as thermodynamically stable, isotropically clear dispersions of two immiscible liquids that are stabilized by interfacial films of surface-active molecules (Leung and Shah, in: Cont~°olled Release of D~~ugs: Polymer s and Aggf~egate Systems, Rosoff, M., Ed., 1989, VCH
Publishers, New York, pages 1852-5). Microemulsions commonly are prepared via a combination of three to five components that include oil, water, surfactant, cosurfactant and electrolyte. Whether the microemulsion is of the water-in-oil (w/o) or an oil-in-water (o/w) type is dependent on the properties of the oil and surfactant used and on the structure and geometric packing of the polar heads and hydrocarbon tails of the surfactant molecules (Schott, in Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, PA, 1985, p. 271).
[0062] The phenomenological approach utilizing phase diagrams has been extensively studied and has yielded a comprehensive knowledge, to one skilled in the art, of how to formulate microemulsions (Rosoff, in Pharmaceutical Dosage Forrns, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New Yorlc, N.Y., volume 1, p. 245; Block, in Pharmaceutical Dosage Fornas, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Deklcer, Inc., New Yorlc, N.Y., volume 1, p. 335). Compared to conventional emulsions, microemulsions offer the advantage of solubilizing water-insoluble drugs in a formulation of thermodynamically stable droplets that are formed spontaneously.
[0063] Surfactants used in the preparation of microemulsions include, but are not limited to, ionic surfactants, non-ionic surfactants, Brij 96, polyoxyethylene oleyl ethers, polyglycerol fatty acid esters, tetraglycerol monolaurate (ML310), tetraglycerol monooleate (M0310), hexaglycerol monooleate (PO310), hexaglycerol pentaoleate (PO500), decaglycerol monocaprate (MCA750), decaglycerol monooleate (M0750),,decaglycerol sequioleate (50750), decaglycerol decaoleate (DA0750), alone or in combination with cosurfactants. The cosurfactant, usually a short-chain alcohol such as ethanol, 1-propanol, and 1-butanol, serves to increase the interfacial fluidity by penetrating into the surfactant film and consequently creating a disordered film because of the void space generated among surfactant molecules. Microemulsions may, however, be prepared without the use of cosurfactants and alcohol-free self emulsifying microemulsion systems are known in the art. The aqueous phase may typically be, but is not limited to, water, an aqueous solution of the drug, glycerol, PEG300, PEG400, polyglycerols, propylene glycols, and derivatives of ethylene glycol. The oil phase may include, but is not limited to, materials such as Captex 300, Captex 355, Capmul MCM, fatty acid esters, medium chain (C8-C 12) mono, di, and triglycerides, polyoxyethylated glyceryl fatty acid esters, fatty alcohols, polyglycolized glycerides, saturated polyglycolized C8-C 10 glycerides, vegetable oils and silicone oil.
[0064] Microemulsions are particularly of interest from the standpoint of drug solubilization and the enhanced absorption of drugs. Lipid based microemulsions (both o/w and w/o) have been proposed to enhance the oral bioavailability of drugs, including peptides (Constantinides et al., Plzar~aaceutical Research, 1994, 11, 1385-1390; R.itschel, Meth. Find. Exp.
Clin. Plaa3-macol., 1993, 13, 205). Microemulsions afford advantages of improved drug solubilization, protection of drug from enzymatic hydrolysis, possible enhancement of drug absorption due to surfactant-induced alterations in membrane fluidity and permeability, ease of preparation, ease of oral administration over solid dosage forms, improved clinical potency, and decreased toxicity (Constantinides et al., Phas~maceutical Research, 1994, 1 l, 1385; Ho et al., J. Pha~m. Sci., 1996, 85, 138-143). Often microemulsions may form spontaneously when their components are brought together at ambient temperature. This may be particularly advantageous when formulating thermolabile drugs, peptides or oligonucleotides. Microemulsions have also been effective in the transdermal delivery of active components in both cosmetic and pharmaceutical applications. It is expected that the microemulsion compositions and formulations of the present invention will facilitate the increased systemic absorption of oligonucleotides and nucleic acids from the gastrointestinal tract, as well as improve the local cellular uptake of oligonucleotides and nucleic acids within the gastrointestinal tract, vagina, buccal cavity and other areas of administration.
[0065] Microemulsions of the present invention may also contain additional components and additives such as sorbitan monostearate (Grill 3), Labrasol, and penetration enhancers to improve the properties of the formulation and to enhance the absorption of the oligonucleotides and nucleic acids of the present invention. Penetration enhancers used in the microemulsions of the present invention may be classified as belonging to one of five broad categories - surfactants, fatty acids, bile salts, chelating agents, and non-chelating non-surfactants (Lee et al., Critical Reviews irz.
Therapeutic Ds°ug Caf-rie~ Systerras, 1991, p. 92). Each of these classes has been discussed above.
Liposomes [0066] There are many organized surfactant structures besides microemulsions that have been studied and used for the formulation of drugs. These include monolayers, micelles, bilayers and vesicles. Vesicles, such as liposomes, have attracted great interest because of their specificity and the duration of action they offer fiom the standpoint of drug delivery.
As used in the present invention, the term "liposome" means a vesicle composed of amphiphilic lipids arranged in a spherical bilayer or bilayers.
[0067] Liposomes are unilamellar or multilamellar vesicles which have a membrane formed from a lipophilic material and an aqueous interior. The aqueous portion contains the composition to be delivered. Cationic liposomes possess the advantage of being able to fuse to the cell wall.

Noncationic liposomes, although not able to fuse as efficiently with the cell wall, are taken up by macrophages in vivo.
[0068] In order to cross intact mammalian skin, Iipid vesicles must pass through a series of fine pores, each with a diameter less than 50 nm, under the influence of a suitable transdermal gradient. Therefore, it is desirable to use a liposome, which is highly deformable and able to pass through such fine pores.
[0069] Further advantages of liposomes include; liposomes obtained from natural phospholipids are biocompatible and biodegradable; liposomes can incorporate a wide range of water and lipid soluble drugs; liposomes can protect encapsulated drugs in their internal compartments from metabolism and degradation (Rosoff, in Phar-rnaceutical Dosage Foams, Lieberman, Rieger and Banker (Eds.), 1988, Marcel Dekker, Inc., New York, N.Y., volume 1, P. 245). Important considerations in the preparation of liposome formulations are the lipid surface charge, vesicle size and the aqueous volume of the liposomes.
[0070] Liposomes are useful for the transfer and delivery of active ingredients to the site of action. Because the liposomal membrane is structurally similar to biological membranes, when liposomes are applied to a tissue, the liposomes start to merge with the cellular membranes. As the merging of the liposome and cell progresses, the liposomal contents are emptied into the cell where the active agent may act.
[0071] Liposomal formulations have been the focus of extensive investigation as the mode of delivery for many drugs. There is growing evidence that for topical administration, liposomes present several advantages over other formulations. Such advantages include reduced side-effects related to high systemic absorption of the administered drug, increased accumulation of the administered drug at the desired target, and the ability to administer a wide variety of drugs, both hydrophilic and hydrophobic, into the skin.
[0072] Several reports have detailed the ability of liposomes to deliver agents including high-molecular weight DNA into the skin. Compounds including analgesics, antibodies, hormones and high-molecular weight DNAs have been administered to the skin. The majority of applications resulted in the targeting of the upper epidermis.
[0073] Liposomes fall into two broad classes. Cationic liposomes are positively charged liposomes, which interact with the negatively charged DNA molecules to form a stable complex. The positively charged DNA/liposome complex binds to the negatively charged cell surface and is internalized in an endosome. Due to the acidic pH within the endosome, the liposomes are ruptured, releasing their contents into the cell cytoplasm (Wang et al., BiocIZem. Biophys. Res. Con2mu~z., 1987, 147, 980 - 985) [0074] Liposomes, which are pH-sensitive or negatively charged, entrap DNA rather than complex with it. Since both the DNA and the lipid axe similarly charged, repulsion rather than complex formation occurs.
Nevertheless, some DNA is entrapped within the aqueous interior of these liposomes. pH-sensitive liposomes have been used to deliver DNA encoding the thymidine kinase gene to cell monolayers in culture. Expression of the exogenous gene was detected in the target cells (Zhou et al., Journal of Contf-olled Release, 1992, 19, 269-274).

[0075] One major type of liposomal composition includes phospholipids other than naturally derived phosphatidylcholine. Neutral liposome compositions, for example, can be formed from dimyristoyl phosphatidylcholine (DMPC) or dipalmitoyl phosphatidylcholine (DPPC).
Anionic liposome compositions generally are formed from dimyristoyl phosphatidylglycerol, while anionic fusogenic liposomes are formed primarily from dioleoyl phosphatidylethanolamine (DOPE). Another type of liposomal composition is formed from phosphatidylcholine (PC) such as, for example, soybean PC, and egg PC. Another type is formed from mixtures of phospholipid and/or phosphatidylcholine and/or cholesterol.
[0076] Several studies have assessed the topical delivery of liposomal drug formulations to the skin. Application of liposomes containing interferon to guinea pig skin resulted in a reduction of skin herpes sores while delivery of interferon via other means (e.g. as a solution or as an emulsion) were ineffective (Weiner et al., Jom°nal of Drug Taf getifZg, 1992, 2, 405-410). Further, an additional study tested the efficacy of interferon administered as part of a liposomal formulation to the administration of interferon using an aqueous system, and concluded that the liposomal formulation was superior to aqueous administration (du Plessis et al., Antiviral Research, 1992, 18, 259-265).
[0077] Non-ionic liposomal systems have also been examined to determine their utility in the delivery of drugs to the skin, in particular systems comprising non-ionic surfactant and cholesterol. Non-ionic liposomal formulations comprising Novasome TM I (glyceryl 2S dilaurate/cholesterol/polyoxyethylene-10-stearyl ether) and NovasomeTM II

(glyceryl distearate/ cholesterol/polyoxyethylene-10-stearyl ether) were used to deliver cyclosporin-A into the dermis of mouse skin. Results indicated that such non-ionic liposomal systems were effective in facilitating the deposition of cyclosporin-A into different layers of the skin (Hu et al. S.T.P.Pharma. Sci., 1994, 4, 6, 466).
[0078] Liposomes also include "sterically stabilized" liposomes, a term, which, as used herein, refers to liposomes comprising one or more specialized lipids that, when incorporated into liposomes, result in enhanced circulation lifetimes relative to liposomes lacking such, specialized lipids.
Examples of sterically stabilized liposomes are those in which part of the vesicle-forming lipid portion of the liposome (A) comprises one or more glycolipids, such as monosialoganglioside GMT, or (B) is derivatized with one or more hydrophilic polyners, such as a polyethylene glycol (PEG) moiety. While not wishing to be bound by any particular theory, it is thought in the art that, at least for sterically stabilized liposomes containing gangliosides, sphingomyelin, or PEG-derivatized lipids, the enhanced circulation half life of these sterically stabilized liposomes derives from a reduced uptake into cells of the reticuloendothelial system (RES) (Allen et al., FEBS Letters, 1987, 223, 42; Wu et al., Ca~zcey~ Research, 1993, 53, 3765).
[0079] Various liposomes comprising one or more glycolipids are known in the art. Papahadjopoulos et al. (Alan. N. Y. Acad. Sci., 1987, 507, 64) reported the ability of monosialoganglioside GM,, galactocerebroside sulfate and phosphatidylinositol to improve blood half lives of liposomes.
These findings were expounded upon by Gabizon et al. (Pnoc. Natl. Acad.

Sci. U.S.A., 1988, 85, 6949). U.S. Patent No. 4,837,028 and WO 88/04924, both to Allen et al., disclose liposomes comprising (1) sphingomyelin and (2) the ganglioside Gjor a galactocerebroside sulfate ester. U.S. Patent No.
5,543,152 (Webb et al.) discloses liposomes comprising sphingomyelin.
Liposomes comprising 1,2-sn-dimyristoylphosphatidylcholine are disclosed in WO 97/13499 (Lim et al.).
[0080] Many liposomes comprising lipids derivatized with one or more hydrophilic polymers, and methods of preparation thereof, are known in the art. Sunamoto et al. (Bull. Chem. Soc. Jpn., 1980, 53, 2778) described liposomes comprising a nonionic detergent, 2C,215G that contains a PEG
moiety. Illum et al. (FEBS Lett., 1984, 167, 79) noted that hydrophilic coating of polystyrene particles with polymeric glycols results in significantly enhanced blood half lives. Synthetic phospholipids modified by the attachment of carboxylic groups of polyalkylene glycols (e.g., PEG) are described by Sears (U.S. Patent Nos. 4,426,330 and 4,534,899).
Klibanov et al. (FEBS Lett., 1990, 268, 235) described experiments demonstrating that liposomes comprising phosphatidylethanolamine (PE) derivatized with PEG or PEG stearate have significant increases in blood circulation half lives. Blume et al. (Bioclaifzaica et Biophysics Acta, 1990, 1029, 91) extended such observations to other PEG derivatized phospholipids, e.g., DSPE-PEG, formed from the combination of distearoylphosphatidylethanolamine (DSPE) and PEG. Liposomes having covalently bound PEG moieties on their external surface are described in European Patent No. EP 0 445 131 B1 and WO 90/04384 to Fisher.
Liposome compositions containing 1-20 mole percent of PE derivatized with PEG, and methods ofuse thereof, axe described by Woodle et al. (U.S.
Patent Nos. 5,013,SS6 and 5,356,633) and Martin et al. (U.S. Patent No.
5,213,804 and European Patent No. EP 0 496 813 Bl). Liposomes comprising a number of other lipid-polymer conjugates are disclosed in WO
91/05545 and U.S. Patent No. 5,225,212 (both to Martin et al.) and in WO
94120073 (Zalipsky et al.) Liposomes comprising PEG-modified ceramide lipids are described in WO 96/10391 (Choi et al.). U.S. Patent Nos.
5,540,935 (Miyazalci et al.) and 5,556,948 (Tagawa et al.) describe PEG-containing liposomes that can be further derivatized with functional moieties on their surfaces.
[0081] A limited number of liposomes comprising nucleic acids are known in the art. WO 96/40062 to Thierry et al. discloses methods for encapsulating high molecular weight nucleic acids in liposomes. U.S. Patent No. 5,264,221 to Tagawa et al. discloses protein-bonded liposomes and asserts that the contents of such liposomes may include an antisense RNA.
U.S. Patent No. 5,665,710 to Rahman et al. describes certain methods of encapsulating oligodeoxynucleotides in liposomes. WO 97/04787 to Love et al. discloses liposomes comprising antisense oligonucleotides targeted to the raf gene.
[0082] Transfersomes are yet another type of liposomes, and are highly deformable lipid aggregates which are attractive candidates for drug delivery vehicles. Transfersomes may be described as lipid droplets, which are so highly deformable that they are easily able to penetrate through pores, which are smaller than the droplet. Transfersomes are adaptable to the 2S environment in which they are used, e.g. they axe self optimizing (adaptive to the shape of pores in the skin), self repairing, frequently reach their targets without fragmenting, and often self loading. To make transfersomes it is possible to add surface edge-activators, usually surfactants, to a standard liposomal composition. Transfersomes have been used to deliver serum albumin to the skin. The transfersome-mediated delivery of serum albumin has been shown to be as effective as subcutaneous injection of a solution containing serum albumin.
[0083] Surfactants find wide application in formulations such as emulsions (including microemulsions) and liposomes. The most common way of classifying and ranking the properties of the many different types of surfactants, both natural and synthetic, is by the use of the hydrophile/lipophile balance (HLB). The nature of the hydrophilic group (also known as the "head") provides the most useful means for categorizing the different surfactants used in formulations (Rieger, in Phar°naaceistical Dosage Fo~nas, Marcel Dekker, Inc., New York, NY, 1988, p. 285) [0084] If the surfactant molecule is not ionized, it is classified as a nonionic surfactant. Nonionic surfactants fmd wide application in pharmaceutical and cosmetic products and are usable over a wide range of pH values. In general their HLB values range from 2 to about 18 depending on their structure. Nonionic surfactants include nonionic esters such as ethylene glycol esters, propylene glycol esters, glyceryl esters, polyglyceryl esters, sorbitan esters, sucrose esters, and ethoxylated esters. Nonionic alkanolamides and ethers such as fatty alcohol ethoxylates, propoxylated alcohols, and ethoxylated/propoxylated block polymers are also included in this class. The polyoxyethylene surfactants are the most popular members of the nonionic surfactant class.
[0085] If the surfactant molecule carries a negative charge when it is dissolved or dispersed in water, the surfactant is classified as anionic.
Anionic surfactants include carboxylates such as soaps, acyl lactylates, acyl amides of amino acids, esters of sulfuric acid such as alkyl sulfates and ethoxylated alkyl sulfates, sulfonates such as alkyl benzene sulfonates, acyl isethionates, aryl taurates and sulfosuccinates, and phosphates. The most important members of the anionic surfactant class are the alkyl sulfates and the soaps.
[0086) If the surfactant molecule carries a positive charge when it is dissolved or dispersed in water, the surfactant is classified as cationic.
Cationic surfactants include quaternary ammonium salts and ethoxylated amines. The quaternary ammonium salts are the most used members of this class.
[0087] If the surfactant molecule has the ability to carry either a positive or negative charge, the surfactant is classified as amphoteric. Amphoteric surfactants include acrylic acid derivatives, substituted alkylamides, N-alkylbetaines and phosphatides.
[0088] The use of surfactants in drug products, formulations and in emulsions has been reviewed (Rieger, in Pharmaceutical Dosage Forms, Marcel Dekker, Inc., New York, NY, 1988, p. 285). Penetration Enhancers [0089] In one embodiment, the present invention employs various penetration enhancers to effect the efficient delivery of nucleic acids particularly oligonucleotides, to the skin of animals. Most drugs are present in solution in both ionized and nonionized forms. However, usually only lipid soluble or lipophilic drugs readily cross cell membranes. It has been discovered that even non-lipophilic drugs may cross cell membranes if the membrane to be crossed is treated with a penetration enhancer. In addition to aiding the diffusion of non-lipophilic drugs across cell membranes, penetration enhancers also enhance the permeability of lipophilic drugs.
[0090] Penetration enhancers may be classified as belonging to one of five broad categories, i.e., surfactants, fatty acids, bile salts, chelating agents, and non-chelating nonsurfactants (Lee et al., Critical Reviews iu Therapeutic Drug Carrier Systems, 1991, p.92). Each of the above mentioned classes of penetration enhancers are described below in greater detail.
[0091] Surfactants: In connection with the present invention, surfactants (or "surface-active agents") are chemical entities which, when dissolved in an aqueous solution, reduce the surface tension of the solution or the interfacial tension between the aqueous solution and another liquid, with the result that absorption of oligonucleotides through the mucosa is enhanced.
In addition to bile salts and fatty acids, these penetration enhancers include, for example, sodium lauryl sulfate, polyoxyethylene-9-lauryl ether and polyoxyethylene-20-cetyl ether) (Lee et al., Critical Reviews in Therapeutic Drug Carrier Systems, 1991, p.92); and perfluorochemical emulsions, such as FC-43. Takahashi et al., J. Pharm. Pharmacol., 1988, 40, 252).
[0092] Fatty acids: Various fatty acids and their derivatives which act as penetration enhancers include, for example, oleic acid, lauric acid, capric acid (n-decanoic acid), myristic acid, palmitic acid, stearic acid, linoleic acid, linolenic acid, dicaprate, tricaprate, monoolein (1-monooleoyl-.rac-glycerol), dilaurin, caprylic acid, arachidonic acid, glycerol 1-monocaprate, 1-dodecylazacycloheptan-2-one, acylcarnitines, acylcholines, C,_~o alkyl esters thereof (e.g., methyl, isopropyl and t-butyl), and mono- and di-glycerides thereof (i.e., oleate, laurate, caprate, myristate, palmitate, stearate, linoleate, etc.) (Lee et al., Critical Reviews in Thef°apeutic D~°ug Cap°rier Systef~as, 1991, p.92; Muranishi, Critical Reviews in Therapeutic Drug Carrier Systems, 1990, 7, 1-33; El Hariri et al., J. Phaf°na.
Pharmacol., 1992, 44, 651-654).
[0093] Bile salts: The physiological role of bile includes the facilitation of dispersion and absorption of lipids and fat-soluble vitamins (Brunton, Chapter 38 in: Goodman & Gilman's The Pharmacological Basis of They°apeutics, 9th Ed., Hardman et al. Eds. McGraw-Hill, New York, 1996, pp. 934-935). Various natural bile salts, and their synthetic derivatives, act as penetration enhancers. Thus the term "bile salts" includes any of the naturally occurring components of bile as well as any of their synthetic derivatives. The bile salts of the invention include, for example, cholic acid (or its pharmaceutically acceptable sodium salt, sodium cholate), dehydrocholic acid (sodium dehydrocholate), deoxycholic acid (sodium deoxycholate), glucholic acid (sodium glucholate), glycholic acid (sodium glycocholate), glycodeoxycholic acid (sodium glycodeoxycholate), taurocholic acid (sodium taurocholate), taurodeoxycholic acid (sodium taurodeoxycholate), chenodeoxycholic acid (sodium chenodeoxycholate), ursodeoxycholic acid (UDCA), sodium tauro-24,25-dihydro-fusidate (STDHF), sodium glycodihydrofusidate'and polyoxyethylene-9-lauryl ether (POE) (Lee et al., Critical Reviews in Tlzerapeutic Drug Carrier Systems, 1991, page 92; Swinyard, Chapter 39 In: Renzirzgton's Pharmaceutical Sciences, 18th Ed., Gennaro, ed., Maclc Publishing Co., Easton, PA, 1990, pages 782-783; Muranishi, Critical Reviews in Therapeutic Drug Carrier Systems, 1990, 7, 1-33; Yamamoto et al., J. Pharnz. Exp. Ther., 1992, 263, 25; Yamashita et al., J. Plzarm. Sci., 1990, 79, 579-583).
[0094] Chelating Agents: Chelating agents, as used in connection with the present invention, can be defined as compounds that remove metallic ions from solution by forming complexes therewith, with the result that absorption of oligonucleotides through the mucosa is enhanced. With regards to their use as penetration enhancers in the present invention, chelating agents have the added advantage of also serving as DNase inhibitors, as most characterized DNA nucleases require a divalent metal ion for catalysis and are thus inhibited by chelating agents (Jarrett, J.
Chr°omatogr°., 1993, 618, 315-339). Chelating agents of the invention include but are not limited to disodium. ethylenediaminetetraacetate (EDTA), citric acid, salicylates (e.g., sodium salicylate, 5-methoxysalicylate and homovanilate), N-acyl derivatives of collagen, laureth-9 and N-amino acyl derivatives of beta-diketones (enamines)(Lee et al., Critical Reviews irz Therapeutic Dr°ug Car°r°ier Systems, 1991, page 92;
Muranishi, CT°itical Reviews in Therapeutic Drug Carrier Systenzs, 1990, 7, 1-33; Buur et al., .I.
Control Rel., 1990, 14, 43-51).
[0095] Non-chelating non-surfactants: As used herein, nonchelating non-surfactant penetration enhancing compounds can be defined as compounds that demonstrate insignificant activity as chelating agents or as surfactants but that nonetheless enhance absorption of oligonucleotides through the alimentary mucosa (Muranishi, C~°itical Reviews in Therapeutic Drug Carrier Systems, 1990, 7, 1-33). This class of penetration enhancers include, for example, unsaturated cyclic ureas, 1-alkyl- and 1-alkenylazacyclo-alkanone derivatives (Lee et al., Critical Reviews ih Therapeutic D~°ug Carrier Systefns, 1991, page 92); and non-steroidal anti-inflammatory agents such as diclofenac sodium, indomethacin and phenylbutazone (Yamashita et al., J. Pharm. Pharmacol., 1987, 39, 621-626).
[0096] Agents that enhance uptake of oligonucleotides at the cellular level may also be added to the pharmaceutical and other compositions of the present invention. For example, cationic lipids, such as lipofectin (Junichi et al, U.S. Patent No. 5,705,188), cationic glycerol derivatives, and polycationic molecules, such as polylysine (Lollo et al., PCT Application WO 97/30731), are also known to enhance the cellular uptake of oligonucleotides.
[0097] Other agents may be utilized to enhance the penetration of the administered nucleic acids, including glycols such as ethylene glycol and propylene glycol, pyrrols such as 2-pyrrol, azones, and terpenes such as limonene and menthone.
Carriers [0098] Certain compositions of the present invention also incorporate carrier compounds in the formulation. As used herein, "carrier compound"
or "carrier" can refer to a nucleic acid, or analog thereof, which is inert (i.e., does not possess biological activity per se) but is recognized as a nucleic acid by in vivo processes that reduce the bioavailability of a nucleic acid having biological activity by, for example, degrading the biologically active nucleic acid or promoting its removal from circulation. The coadministration of a nucleic acid and a carrier compound, typically with an excess of the latter substance, can result in a substantial reduction of the amount of nucleic acid recovered in the liver, kidney or other extracirculatory reservoirs, presumably due to competition between the carrier compound and the nucleic acid for a common receptor. For example, the recovery of a partially phosphorothioate oligonucleotide in hepatic tissue can be reduced when it is coadministered with polyinosinic acid, dextran sulfate, polycytidic acid or 4-acetamido-4'isothiocyano-stilbene-2,2' disulfonic acid (Miyao et al., Azztisense Res. Dev., 1995, 5, 115-121;
Takakura et al., Azztisense & Nucl. Acid Dz°ug Dev., 1996, 6, 177-183).
Excipients [0099] In contrast to a carrier compound, a "pharmaceutical carrier" or "excipient" is a pharmaceutically acceptable solvent, suspending agent or any other pharmacologically inert vehicle for delivering one or more nucleic acids to an animal. The excipient may be liquid or solid and is selected, with the planned manner of administration in mind, so as to provide~for the desired bulk, consistency, etc., when combined with a nucleic acid and the other components of a given pharmaceutical composition. Typical pharmaceutical carriers include, but are not limited to, binding agents (e.g., pregelatinized maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose, etc.); fillers (e.g., lactose and other sugars, microcrystalline cellulose, pectin, gelatin, calcium sulfate, ethyl cellulose, polyacrylates or so calcium hydrogen phosphate, etc.); lubricants (e.g., magnesium stearate, talc, silica, colloidal silicon dioxide, stearic acid, metallic stearates, hydrogenated vegetable oils, coin starch, polyethylene glycols, sodium benzoate, sodium acetate, etc.); disintegr ants (e.g., starch, sodium starch glycolate, etc.); and wetting agents (e.g., sodium lauryl sulphate, etc.).
[00100] Pharmaceutically acceptable organic or inorganic excipient suitable for non-parenteral administration, which does not deleteriously react with nucleic acids, can also be used to formulate t1e compositions of the present invention. Suitable pharmaceutically acceptable carriers include, but are not limited to, water, salt solutions, alcohols, polyethylene glycols, gelatin, lactose, amylose, magnesium stearate, talc, silicic acid, viscous paraffin, hydroxymethylcellulose, polyvinylpyrrolidone and the like.
[00101] Formulations for topical administration of nucleic acids may include sterile and non-sterile aqueous solutions, non-aqueous solutions in common solvents such as alcohols, or solutions of the nucleic acids in liquid or solid oil bases. The solutions may also contain buffers, diluents and other suitable additives. Pharmaceutically acceptable organic or inorganic excipients suitable for non-parenteral administration, which do not deleteriously react with nucleic acids, can be used.
[00102] Suitable pharmaceutically acceptable excipients include, but are not limited to, water, salt solutions, alcohol, polyethylene glycols, gelatin, lactose, amylose, magnesium stearate, talc, silicic acid, viscous paraffin, hydroxymethylcellulose, polyvinylpyrrolidone and the like.
Other Components s~

[00103] The compositions of the present invention may additionally contain other adjunct components conventionally found in pharmaceutical compositions, at their art-established usage levels. Thus, for example, the compositions may contain additional, compatible, pharmaceutically-active materials such as, for example, antipruritics, astringents, local anesthetics or anti-inflammatory agents, or may contain additional materials useful in physically formulating various dosage forms of the compositions of the present invention, such as dyes, flavoring agents, preservatives, antioxidants, opacifiers, thickening agents and stabilizers. However, such materials, when added, should not unduly interfere with the biological activities of the components of the compositions of the present invention.' The formulations can be sterilized and, if desired, mixed with auxiliary agents, e.g., lubricants, preservatives, stabilizers, wetting agents, emulsifiers, salts for influencing osmotic pressure, buffers, colorings, flavorings and/or aromatic substances and the like which do not deleteriously interact with the nucleic acids) of the formulation.
[00104] Aqueous suspensions may contain substances, which increase the viscosity of the suspension including, for example, sodium carboxymethylcellulose, sorbitol, and/or dextran. The suspension may also contain stabilizers.
[00105] Certain embodiments of the invention provide pharmaceutical compositions containing (a) one or more antisense compounds and (b) one or more other chemotherapeutic agents which function by a non-antisense mechanism. Examples of such chemotherapeutic agents include, but are not limited to, anticancer drugs such as daunorubicin, dactinomycin, s2 doxorubicin, bleomycin, mitomycin, nitrogen mustard, chlorambucil, melphalan, cyclophosphamide, 6-mercaptopurine, 6-thioguanine, cytarabine (CA), S-fluorouracil (S-FU), floxuridine (S-FUdR), methotrexate (MTX), colchicine, vincristine, vinblastine, etoposide, teniposide, cisplatin and S diethylstilbestrol (DES). See, generally, The Mef~ck Manual of Diagnosis and Thef°apy, 15th Ed., Berkow et al., eds., 1987, Rahway, N.J., pages 1228). Anti-inflammatory drugs, including but not limited to nonsteroidal anti-inflammatory drugs and corticosteroids, and antiviral drugs, including but not limited to ribivirin, vidarabine, acyclovir and ganciclovir, may also be combined in compositions of the invention. See, generally, The Merck Ma3aual of Diagnosis afad Therapy, 1 Sth Ed., Berkow et al., eds., 1987, Rahway, N.J., pages 2499-2506 and 46-49, respectively). other non-antisense chemotherapeutic agents are also within the scope of this invention. Two or more combined compounds may be used together or 1 S sequentially.
[00106] In another related embodiment, compositions of the invention may contain one or more antisense compounds, particularly oligonucleotides, targeted to a first nucleic acid and one or more additional antisense compounds targeted to a second nucleic acid target. Numerous examples of antisense compounds are known in the art. Two or more combined compounds may be used together or sequentially.
[00107] The formulation of therapeutic compositions and their subsequent administration is believed to be within the skill of those in the art. Dosing is dependent on severity and responsiveness of the disease state 2S to be treated, with the course of treatment lasting from several days to several months, or until a cure is effected or a diminution of the disease state is achieved. Optimal dosing schedules can be calculated from measurements of drug accumulation in the body of the patient. Persons of ordinary skill can easily determine optimum dosages, dosing methodologies and repetition rates. Optimum dosages may vary depending on the relative potency of individual oligonucleotides, and can generally be estimated based on ECsos found to be effective in in vitro and in viva animal models. In general, dosage is from 0.01 ~.g to 100 g per kg of body weight, and may be given once or more daily, weekly, monthly or yearly, or even once every 2 to 20 years. Persons of ordinary skill in the art can easily estimate repetition rates for dosing based on measured residence times and concentrations of the drug in bodily fluids or tissues. Following successful treatment, it may be desirable to have the patient undergo maintenance therapy to prevent the recurrence of the disease state, wherein the oligonucleotide is administered in maintenance doses, ranging from 0.01 ~.g to 100 g per kg of body weight, once or more daily, to once every 20 years.
[00108] While the present invention has been described with specificity in accordance with certain of its preferred embodiments, the following examples serve only to illustrate the invention and are not intended to limit the same.

EXAMPLES
Example 1 Nucleoside Phosphoramidites for Oligonucleotide Synthesis Deoxy and 2'-alkoxy amidites (00109] 2'-Deoxy and 2'-methoxy beta-cyanoethyldiisopropyl phosphoramidites are available from commercial sources (e.g. Chemgenes, Needham MA or Glen Research, Inc. Sterling VA). Other 2'-O-alkoxy substituted nucleoside amidites are prepared as described in U.S. Patent 5,506,351, herein incorporated by reference. For oligonucleotides synthesized using 2'-alkoxy amidites, the standard cycle for unmodified oligonucleotides is utilized, except the wait step after pulse delivery of tetrazole and base is increased to 360 seconds.
[00110] Oligonucleotides containing 5-methyl-2'-deoxycytidine (5-Me-C) nucleotides are synthesized according to published methods [Sanghvi, et.
al., Nucleic Acids Research, 1993, 21, 3197-3203] using commercially available phosphoramidites (Glen Research, Sterling VA or ChemGenes, Needham MA).
2'-Fluoro amidites 2'-Fluorodeoxyadenosine amidites [00111] 2'-fluoro oligonucleotides are synthesized as described previously [Kawasaki, et. al., J. Med. Claena., 1993, 36, 831-841] and United States patent 5,670,633, herein incorporated by reference. Briefly, the protected nucleoside N6-benzoyl-2'-deoxy-2'-fluoroadenosine is synthesized utilizing commercially available 9-beta-D-arabinofuranosyladenine as starting material and by modifying literature procedures whereby the 2'-alpha-fluoro atom is introduced by a SN2-displacement of a 2'-beta-trityl group. Thus N6-benzoyl-9-beta-D-arabinofuranosyladenine is selectively protected in moderate yield as the 3',5'-ditetrahydropyranyl (THP) intermediate. Deprotection of the THP and N6-benzoyl groups is accomplished using standard methodologies and standard methods are used to obtain the 5'-dimethoxytrityl-(DMT) and 5'-DMT-3'-phosphoramidite intermediates.
2'-Fluorodeoxyguanosine [00112] The synthesis of 2'-deoxy-2'-fluoroguanosine is accomplished using tetraisopropyldisiloxanyl (TPDS) protected 9-beta-D-arabinofuranosylguanine as starting material, and conversion to the intermediate diisobutyrylarabinofuranosylguanosine. Deprotection of the TPDS group is followed by protection of the hydroxyl group with THP to give diisobutyryl di-THP protected arabinofuranosylguanine. Selective O-deacylation and triflation is followed by treatment of the crude product with fluoride, then deprotection of the THP groups. Standard methodologies are used to obtain the 5'-DMT- and 5'-DMT-3'-phosphoramidites.
2'-Fluorouridine [00113] Synthesis of 2'-deoxy-2'-fluorouridine is accomplished by the modification of a literature procedure in which 2,2'anhydro-1-beta-D-arabinofuranosyluracil is treated with 70% hydrogen fluoride-pyridine.
Standard procedures are used to obtain the 5'-DMT and 5'-DMT-3'-phosphoramidites.

2'-Fluorodeoxycytidine [00114] 2'-deoxy-2'-fluorocytidine is synthesized via amination of 2'-deoxy-2'-fluorouridine, followed by selective protection to give N4-benzoyl-2'-deoxy-2'-fluorocytidine. Standard procedures are used to obtain the 5'-DMT and 5'-DMT-3'phosphoramidites.
2'-O-(2-Methoxyethyl) modified amidites [00115] 2'-O-Methoxyethyl-substituted nucleoside amidites are prepared as follows, or alternatively, as per the methods of Martin, F., Helvetica Chirnica Acta, 1995, 78, 486-504.
2,2'-Anhydro[1-(beta-D-arabinofuranosyl)-5-methyluridinel [00116] 5-Methyluridine (ribosylthymine, commercially available through Yamasa, Choshi, Japan) (72.0 g, 0.279 M), diphenylcarbonate (90.0 g, 0.420 M) and sodium bicarbonate (2.0 g, 0.024 M) are added to DMF
(300 mL). The mixture is heated to reflux, with stirnng, allowing the evolved carbon dioxide gas to be released in a controlled manner. After 1 hour, the slightly darkened solution is concentrated under reduced pressure.
The resulting syrup is poured into diethylether (2.5 L), with stirring. The product formed a gum. The ether is decanted and the residue is dissolved in a minimum amount of methanol (ca. 400 mL). The solution is poured into fresh ether (2.5 L) to yield a stiff gum. The ether is decanted and the gum is dried in a vacuum oven (60°C at 1 mm Hg for 24 h) to give a solid that is crushed to a light tan powder. The material is used as is for further reactions (or it can be purified further by column chromatography using a gradient of methanol in ethyl acetate (10-25%) to give a white solid.
2'-O-Methoxyethyl-5-methyluridine s~

[00117] 2,2'-Anhydro-5-methyluridine (195 g, 0.81 M), tris(2-methoxyethyl)borate (231 g, 0.98 M) and 2-methoxyethanol (1.2 L) are added to a 2 L stainless steel pressure vessel and placed in a pre-heated oil bath at 160°C. After heating for 48 hours at 155-160°C, the vessel is opened and the solution evaporated to dryness and triturated with MeOH (200 mL).
The residue is suspended in hot acetone (1 L). The insoluble salts are filtered, washed with acetone (150 mL) and the filtrate evaporated. The residue (280 g) is dissolved in CH3CN (600 mL) and evaporated. A silica gel column (3 lcg) is packed in CH2Cl2 /acetone /MeOH (20:5:3) containing 0.5% Et3NH. The residue is dissolved in CHzCl2 (250 mL) and adsorbed onto silica (150 g) prior to loading onto the column. The product is eluted with the packing solvent to give the title product. Additional material can be obtained by reworking impure fractions.
2'-O-Methoxyethyl-5'-O-dimethoxytrityl-5-methyluridine [00118] 2'-O-Methoxyethyl-5-methyluridine (160 g, 0.506 M) is co-evaporated with pyridine (250 mL) and the dried residue dissolved in pyridine (1.3 L). A first aliquot of dimethoxytrityl chloride (94.3 g, 0.278 M) is added and the mixture stirred at room temperature for one hour. A
second aliquot of dimethoxytrityl chloride (94.3 g, 0.278 M) is added and the reaction stirred for an additional one hour. Methanol (170 mL) is then added to stop the reaction. The solvent is evaporated and triturated with CH3CN (200 mL) The residue is dissolved in CHCl (1.5 L) and extracted with 2x500 mL of saturated NaHC03 and 2x500 mL of saturated NaCI. The organic phase is dried over Na2S04, filtered, and evaporated. The residue is purified on a 3.5 kg silica gel column, packed and eluted with EtOAc/hexane/ acetone (5:5:1) containing 0-5% Et3NH. The pure fractions are evaporated to give the title product.
3'-O-Acetyl-2'-O-methoxyethyl-5'-O-dimethoxytrityl-5-methyluridine [00119) 2'-O-Methoxyethyl-5'-O-dimethoxytrityl-5-methyluridine (106 g, 0.167 M), DMF/pyridine (750 mL of a 3:1 mixture prepared from 562 mL
ofDMF and 188 mL ofpyridine) and acetic anhydride (24.38 mL, 0.258 M) are combiiled and stirred at room temperature for 24 hours. The reaction is monitored by TLG by first quenching the TLC sample with the addition of MeOH. Upon completion of the reaction, as judged by TLC, MeOH (50 mL) is added and the mixture evaporated at 35°C. The residue is dissolved in CHC13 (800 mL) and extracted with 2x200 mL of saturated sodium bicarbonate and 2x200 mL of saturated NaCl. The water layers are back extracted with 200 mL of CHC13. The combined organics are dried with sodium sulfate and evaporated to a residue. The residue is purified on a 3.5 kg silica gel column and eluted using EtOAc/hexane(4:1). Pure product fractions are evaporated to yield the title compounds.
3'-O-Acetyl-2'-O-methoxyethyl-5'-O-dimethoxytrityl-5-methyl-4-triazoleuridine [00120) A first solution is prepared by dissolving 3'-.O-acetyl-2'-O-methoxyethyl-5'-O-dimethoxytrityl-5-methyluridine (96 g, 0.144 M) in CH3CN (700 mL) and set aside. Triethylamine (189 mL, 1.44 M) is added to a solution of triazole (90 g, 1.3 M) in CH3CN (1 L), cooled to -5°C and stirred for 0.5 h using an overhead stirrer. POC 13 is added dropwise, over a minute period, to the stirred solution maintained at 0-10°C, and the 25 resulting mixture stirred for an additional 2 hours. The first solution is added dropwise, over a 45 minute period, to the latter solution. The resulting reaction mixture is stored overnight in a cold room. Salts are filtered from the reaction mixture and the solution is evaporated. The residue is dissolved in EtOAc (1 L) and the insoluble solids are removed by filtration. The filtrate is washed with 1x300 mL of NaHC03 and 2x300 mL of saturated NaCI, dried over sodium sulfate and evaporated. The residue is triturated with EtOAc to give the title compound.
2'-O-Methoxyethyl-5'-O-dimethoxytrityl-5-methylcytidine [00121] A solution of 3'-O-acetyl-2'-O-methoxyethyl-5'-O-dimethoxytrityl-5-methyl-4-triazoleuridine (103 g, 0.141 M) in dioxane (500 mL) and NH40H (30 mL) is stirred at room temperature fox 2 hours.
The dioxane solution is evaporated and the residue azeotroped with MeOH
(2x200 mL). The residue is dissolved in MeOH (300 mL) and transferred to a 2 liter stainless steel pressure vessel. MeOH (400 mL) saturated with NH3 gas is added and the vessel heated to 100°C for 2 hours (TLC showed complete conversion). The vessel contents are evaporated to dryness and the residue is dissolved in EtOAc (500 rnL) and washed once with saturated NaCl (200 mL). The organics are dried over sodium sulfate and the solvent is evaporated to give the title compound.
N4-Benzoyl-2'-O-methoxyethyl-5'-O-dimethoxytrityl-5-methylcytidine [00122] 2'-O-Methoxyethyl-5'-O-dimethoxytrityl-5-methylcytidine (85 g, 0.134 M) is dissolved in DMF (800 mL) and benzoic anhydride (37.2 g, 0.165 M) is added with stirring. After stirring for 3 hours, TLC showed the reaction to be approximately 95% complete. The solvent 'is evaporated and the residue azeotroped with MeOH (200 mL). The residue is dissolved in CHC13 (700 mL) and extracted with saturated NaHCO, (2x300 mL) and saturated NaCl (2x300 mL) , dried over MgS04 and evaporated to give a residue. The residue is chromatographed on a I.5 kg silica column using EtOAc/hexane (1:1) containing 0-5% Et3NH as the eluting solvent. The pure product fractions are evaporated to give the title compound.
N4-Benzoyl-Z'-O-methoxyethyl-5'-O-dimethoxytrityl-5-methylcytidine-3'-amidite [00123] N4-Benzoyl-2'-O-methoxyethyl-5'-O-dimethoxytrityl-5-methylcytidine (74 g, 0.10 M) is dissolved in CHZC12 (1 L) Tetrazole diisopropylamine (7.1 g) and 2-cyanoethoxy-tetra(isopropyl)phosphite (40.5 mL, 0.123 M) are added with stirring, under a nitrogen atmosphere. The resulting mixture is stirred for 20 hours at room temperature (TLC showed the reaction to be 95% complete). The reaction mixture is extracted with saturated NaHC03 (1x300 mL) and saturated NaCI (3x300 mL). The aqueous washes are back-extracted with CH2Clz (300 mL), and the extracts are combined, dried over MgSO4 and concentrated. The residue obtained is chromatographed on a 1.5 kg silica column using EtOAc/hexane (3:1 ) as the eluting solvent. The pure fractions were combined to give the title compound.
2'-O-(Aminooxyethyl) nucleoside amidites and 2'-O-(dimethylaminooxyethyl) nucleoside amidites 2'-(Dimethylaminooxyethoxy) nucleoside amidites [00124] 2'-(Dimethylaminooxyethoxy) nucleoside amidites [also known in the art as 2'-O-(dimethylaminooxyethyl) nucleoside amidites] are prepared as described in the following paragraphs. Adenosine, cytidine and guanosine nucleoside amidites axe prepared similarly to the thymidine (5-methyluridine) except the exocyclic amines are protected with a benzoyl moiety in the case of adenosine and cytidine and with isobutyryl in the case of guanosine.
5'-O-tert-Butyldiphenylsilyl -02 -2'-anhydro-5-methyluridine (00125] OZ -2'-anhydro-5-methyluridine (Pro. Bio. Sint., Varese, Italy, 100.Og, 0.4'6 mmol), dimethylaminopyridine (0.66g, 0.013eq, 0.0054mmol) are dissolved in dry pyridine (500 ml) at ambient temperature under an argon atmosphere and with mechanical stirring. tert-Butyldiphenylchlorosilane (125.8g, 119.OmL, l.leq, 0.458mmo1) is added in one portion. The reaction is stirred for 16 h at ambient temperature. TLC
(Rf 0.22, ethyl acetate) indicated a complete reaction. The solution is concentrated under reduced pressure to a thick oil. This is partitioned between dichloromethane (1 L) and saturated sodium bicarbonate (2x1 L) and brine (1 L). The organic layer is dried over sodium sulfate and concentrated under reduced pressure to a thick oil. The oil is dissolved in a 1:1 mixture of ethyl acetate and ethyl ether (600mL) and the solution is cooled to -10°C. The resulting crystalline product is collected by filtration, washed with ethyl ether (3x200 mL), and dried (40°C, lmm Hg, 24 h) to a white solid 5'-O-tert-Butyldiphenylsilyl-2'-O-(2-hydroxyethyl)-5-methyluridine [00126] In a 2 L stainless steel, unstirred pressure reactor is added borane in tetrahydrofuran (1.0 M, 2.0 eq, 622 mL). In the fume hood and with manual stirring, ethylene glycol (350 mL, excess) is added cautiously at first until the evolution of hydrogen gas subsides. 5'-O-tent-Butyldiphenylsilyl-OZ-2'anhydro-5-methyluridine (149 g, 0.3' 1 mol) and sodium bicarbonate (0.074 g, 0.003 ec~ are added with manual stirring. The reactor is sealed and heated in an oil bath until an internal temperature of 160°C is reached and then maintained for 16 h (pressure < 100 psig). The reaction vessel is cooled to ambient and opened. TLC (Rf 0.67 for desired product and Rf 0.82 for ara-T side product, ethyl acetate) indicated about 70% conversion to the product. In order to avoid additional side product formation, the reaction is stopped, concentrated under reduced pressure (10 to lmrn, Hg) in a warm water bath (40-100°C) with the more extreme conditions used to remove the ethylene glycol. [Alternatively, once the low boiling solvent is gone, the remaining solution can be partitioned between ethyl acetate and water. The product will be in the organic phase.] The residue is purified by column chromatography (2kg silica gel, ethyl acetate-hexanes gradient 1:1 to 4:1 ).
The appropriate fractions are combined, stripped and dried to product as a white crisp foam, contaminated starting material, and pure reusable starting material.
2'-O-([2-phthalimidoxy)ethyl]-5'-t-butyldiphenylsilyl-5-methyluridine [00127] 5'-O-tert-Butyldiphenylsilyl-2'-O-(2-hydroxyethyl)-5-methyluridine (20g, 36.98mmol) is mixed with triphenylphosphine (11.63g, 44.36mmo1) and N-hydroxyphthalimide (7.24g, 44.36mmo1). It is then dried over PZOS under high vacuum for two days at 40°C. The reaction mixture is flushed with argon and dry THF (369.8mL, Aldrich, sure seal bottle) is added to get a clear solution. Diethyl-azodicarboxylate (6.98mL, 44.36mmo1) is added dropwise to the reaction mixture. The rate of addition is maintained such that resulting deep red coloration is just discharged before adding the next drop. After the addition is complete, the reaction is stirred for 4 hrs. By that time TLC showed the completion of the reaction (ethylacetate:hexane, 60:40). The solvent is evaporated in vacuum. Residue obtained is placed on a flash column and eluted with ethyl acetate:hexane (60:40), to get 2'-O-([2-phthalimidoxy)ethyl]-5'-t-butyldiphenylsilyl-5-methyluridine as white foam.
5'-O-tart-butyldiphenylsilyl-2'-O-[(2-formadoximinooxy)ethyl]-5-methyluridine [00128] 2'-O-([2-phthalimidoxy)ethyl]-S'-t-butyldiphenylsilyl-5-methyluridine (3.1g, 4.Smmo1) is dissolved in dry CHZC12 (4.SmL) and methylhydrazine (300mL, 4.64mmo1) is added dropwise at-10°C to 0°C.
After 1 h the mixture is filtered, the filtrate is washed with ice cold CH2C12 and the combined organic phase is washed with water, brine and dried over anhydrous Na2S04. The solution is concentrated to get 2'-O(aminooxyethyl) thymidine, which is then dissolved in MeOH (67.SmL). To this formaldehyde (20% aqueous solution, w/w, 1.1 eq.) is added and the resulting mixture is stirred for 1 h. Solvent is removed under vacuum;
residue chromatographed to get S'-O-tent-butyldiphenylsilyl-2'-O-[(2-formadoximinooxy) ethyl]-S-methyluridine as White foam.
5'-O-tart-Sutyldiphenylsilyl-2'-O-[N,N-dimethylaminooxyethyl]-5-methyluridine [40129] 5'-O-tart-butyldiphenylsilyl-2'-O-[(2- formadoximinooxy)ethyl]-S-methyluridine (1.77g, 3.12mmo1) is dissolved in a solution of 1M
pyridinium p-toluenesulfonate (PPTS) in dry MeOH (30.6mL). Sodium cyanoborohydride (0.39g, 6.13mmol) is added to this solution at 10°C
under inert atmosphere. The reaction mixture is stirred for 10 minutes at 10°C.
After that the reaction vessel is removed from the ice bath and stirred at room temperature for 2 h, the reaction monitored by TLC (5% MeOH in CHZC12). Aqueous NaHC03 solution (5%, lOmL) is added and extracted with ethyl acetate (2x20mL). Ethyl acetate phase is dried over anhydrous Na2S04, evaporated to dryness. Residue is dissolved in a solution of 1M
PPTS in MeOH (30.6mL). Formaldehyde (20% w/w, 30mL, 3.37mmo1) is added and the reaction mixture is stirred at room temperature for 10 minutes. Reaction mixture cooled to 10°C in an ice bath, sodium cyanoborohydride (0.39g, 6.13mmo1) is added, and reaction mixture stirred at 10°C for 10 minutes. After 10 minutes, the reaction mixture is removed from the ice bath and stirred at room temperature for 2 hrs. To the reaction mixture 5% NaHCO3 (25mL) solution is added and extracted with ethyl acetate (2x25mL). Ethyl acetate layer is dried over anhydrous Na2SO4 and evaporated to dryness. The residue obtained is purified by flash column chromatography and eluted with 5% MeOH in CHZCh to get 5'-O-tertbutyldiphenylsilyl-2'-O-[N,N-dimethylaminooxyethyl]-5- methyluridine as a white foam.
2'-O-(dimethylaminooxyethyl)-5-methyluridine (00130] Triethylamine trihydrofluoride (3.91mL, 24.Ommo1) is dissolved in dry THF and triethylamine (1.67mL, l2mmol, dry, kept over I~OH). This mixture of triethylamine-2HF is then added to 5'-O-tert-butyldiphenylsilyl-2'-O-[N,N-dimethylaminooxyethyl]-5-methyluridine (1.408, 2.4mmo1) and stirred at room temperature for 24 hrs. Reaction is monitored by TLC (5%
MeOH in CHZCl2). Solvent is removed under vacuum and the residue placed on a flash column and eluted with 10% MeOH in CHZC12 to get 2'-O-(dimethylaminooxyethyl)-5-methyluridine.
5'-O-DMT-2'-O-(dimethylaminooxyethyl)-5-methyluridine [00131] 2'-O-(dimethylaminooxyethyl)-5-methyluridine (750mg, 2.17mmol) is dried over P205 under high vacuum overnight at 40°C. It is then co-evaporated with anhydrous pyridine (20mL). The residue obtained is dissolved in pyridine (llmL) under argon atmosphere. 4-dimethylaminopyridine (26.Smg, 2.60mmo1), 4,4'-dimethoxytrityl chloride (880mg, 2.60mmo1) is added to the mixture and the reaction mixture is stirred at room temperature until all of the starting material disappeared.
Pyridine is removed under vacuum and the residue chromatographed and eluted with 10% MeOH in CH2C12 (containing a few drops of pyridine) to get 5'-O-DMT-2'-0(dimethylamino-oxyethyl)-5-methyluridine.
5'-O-DMT-2'-O-(2-N,N-dimethylaminooxyethyl)-5-methyluridine-3'-[(2-cyanoethyl)-N,N- diisopropylphosphoramidite]
[00132] 5'-O-DMT-2'-O-(dimethylaminooxyethyl)-5-methyluridine (1.08g, 1.67mmo1) is co-evaporated with toluene (20mL). To the residue N,N-diisopropylamine tetrazonide (0.29g, 1.67mmo1) is added and dried over P20, under high vacuum overnight at 40°C. Then the reaction mixture is dissolved in anhydrous acetonitrile (8.4mL) and 2-cyanoethyl-N,N,NI,N~-tetraisopropylphosphoramidite (2.12mL, 6.08mmo1) is added. The reaction mixture is stirred at ambient temperature for 4 hrs under inert atmosphere.
The progress of the reaction is monitored by TLC (hexane:ethyl acetate 1:1).
The solvent is evaporated, then the residue is dissolved in ethyl acetate (70mL) and washed with 5% aqueous NaHC03 (40mL). Ethyl acetate layer is dried over anlrydrous NazS04 and concentrated. Residue obtained is chromatographed (ethyl acetate as eluent) to get S'-O-DMT-2'-O-(2-N,N-dimethylaminooxyethyl)-S-methyluridine-3'-[(2-cyanoethyl)-N,N-diisopropylphosphoramidite] as a foam.
S 2'-(Aminooxyethoxy) nucleoside amidites [00133] 2'-(Aminooxyethoxy) nucleoside amidites [also known in the art as 2'-O-(aminooxyethyl) nucleoside amidites] are prepared as described in the following paragraphs. Adenosine, cytidine and thymidine nucleoside amidites are prepared similarly.
N2-isobutyryl-6-O-diphenylcarbamoyl-2'-O-(Z-ethylacetyl)-5'-O-(4,4'-dimethoxytrityl)guanosine-3'-[(2-cyanoethyl)-N,N-diisopropylphosphoramidite]
[40134] The 2'-O-aminooxyethyl guanosine analog may be obtained by selective 2'-O-alkylation of diaminopurine riboside. Multigram quantities of 1 S diaminopurine riboside may be purchased from Schering AG (Berlin) to provide 2'-O-(2-ethylacetyl) diaminopurine riboside along with a minor amount of the 3'-O-isomer. 2'-O-(2-ethylacetyl) diaminopurine riboside may be resolved and converted to 2'-O-(2ethylacetyl)guanosine by treatment with adenosine deaminase. (McGee, D. P. C., Cook, P. D., Guinosso, C. J., WO 94/02501 A1940203.) Standard protection procedures should afford 2'-O-(2-ethylacetyl)-S'-O-(4,4'-dimethoxytrityl)guanosine and 2-N-isobutyryl-6-O-diphenylcarbamoyl-2'-O-(2-ethylacetyl)-S'-O-(4,4'-dimethoxytrityl)guanosine which may be reduced to provide 2-N-isobutyryl-6-O-diphenylcarbamoyl-2'-O-(2-ethylacetyl)-S'-O-(4,4'-2S dimethoxytrityl)guanosine. As before the hydroxyl group may be displaced by N-hydroxyphthalimide via a Mitsunobu reaction, and the protected nucleoside may phosphitylated as usual to yield 2-N-isobutyryl-6-O-diphenylcarbamoyl-2'-O-(2-ethylacetyl)-5'-O-(4,4'-dimethoxytrityl)guanosine-3'-[(2-cyanoethyl)-N,N-diisopropylphosphoramiditel.
2'-dimethylaminoethoxyethoxy (2'-DMAEOE) nucleoside amidites [00135] 2'-dimethylaminoethoxyethoxy nucleoside amidites (also known in the art as 2'-O-dimethylaminoethoxyethyl, i.e., 2'O-CH2-O-CH2-N(CH2)Z, or 2'-DMAEOE nucleoside amidites) are prepared as follows.
Other nucleoside amidites are prepared similarly.
2'-O-[2(2-N,N-dimethylaminoethoxy)ethyl]-5-methyl uridine j00136] 2[2-(Dimethylamino)ethoxylethanol (Aldrich, 6.66 g, 50 mmol) is slowly added to a solution of borane in tetrahydrofuran (1 M, 10 mL, 10 mmol) with stirring in a 100 mL bomb. Hydrogen gas evolves as the solid dissolves. 02-, 2' - anhydro-5-methyluridine (1.2 g, 5 mmol), and sodium bicarbonate (2.5 mg) are added and the bomb is sealed, placed in an oil bath, and heated to 155°C for 26 hours. The bomb is cooled to room temperature and opened. The crude solution is concentrated and the residue partitioned between water (200 mL) and hexanes (200 mL). The excess phenol is extracted into the hexane layer. The aqueous layer is extracted with ethyl acetate (3x200 mL) and the combined organic layers are washed once with water, dried over anhydrous sodium sulfate and concentrated. The residue is columned on silica gel using methanol/methylene chloride 1:20 (which has 2% triethylamine) as the eluent. As the column fractions are concentrated a colorless solid forms which is collected to give the title compound as a white solid.
5'-O-dimethoxytrityl-2'-O-[2(2-N,N-dimethylaminoethoxy) ethyl)]-S-methyl uridine [00137] To 0.5 g (1.3 mmol) of 2'-O-[2(2-N,N-dimethylaminoethoxy)ethyl)1-5-methyl uridine in anhydrous pyridine (8 mL), triethylamine (0.36 mL) and dimethoxytrityl chloride (DMT-Cl, 0.87 g, 2 eq.) are added and stirred for 1 hour. The reaction mixture is poured into water (200 mL) and extracted with CH2C12 (2x200 mL). The combined CHZC12 layers are washed with saturated NaHC03 solution, followed by saturated NaCI solution and dried over anhydrous sodium sulfate.
Evaporation of the solvent followed by silica gel chromatography using MeOH: CH2C12:Et3N (20:1, v/v, with 1% triethylamine) gives the title compound.
5'-O-Dimethoxytrityl-2'-O-[2(2-N,N-dimethylaminoethoxy)ethyl)]-5-methyl uridine-3'-O-(cyanoethyl-N,N-diisopropyl)phosphoramidite [00138] Diisopropylaminotetrazolide (0.6 g) and 2-cyanoethoxyN,N-diisopropyl phosphoramidite (1.1 mL, 2 eq.) are added to a solution of 5'-O-dimethoxytrityl-2'-O-[2(2-N,N-dimethylaminoethoxy)ethyl)]-5-methyluridine (2.17 g, 3 mmol) dissolved in CHZC12 (20 mL) under an atmosphere of argon. The reaction mixture is stirred overnight and the solvent evaporated. The resulting residue is purified by silica gel flash column chromatography with ethyl acetate as the eluent to give the title compound.

5106'7-55 Example 2 Oligonucleotide synthesis [00139] Unsubstituted and substituted phosphodiester (P=O) oligonucleotides are synthesized on an automated DNA synthesizer (Applied Biosystems model 380B) using standard phosphoramidite chemistry with oxidation by iodine.
[00140] Phosphorothioates (P=S) are synthesized as for the phosphodiester oligonucleotides except the standard oxidation bottle is replaced by 0.2 M solution of 3H-1,2-benzodithiole-3-one 1,1-dioxide in acetonitrile for the stepwise thiation of the phosphite linkages. The thiation wait step is increased to 68 sec and is followed by the capping step. After cleavage from the CPG column and deblocking in concentrated ammonium hydroxide at 55'C (18 h), the oligonucleotides are purified by precipitating twice with 2.5 volumes of ethanol from a 0.5 M NaCI solution. Phosphinate oligonucleotides are prepared as described in U.S. Patent 5,508,70 .
[00141) Alkyl phosphonate oligonucleotides are prepared as described in U.S. Patent 4,469,863, herein incorporated by reference.
[00142] 3'-Deoxy-3'-methylene phosphonate oligonucleotides are prepared as described in U.S. Patents 5,610,289 or 5,625,050 .
[00143] Phosphoranudite oligonucleotides are prepared as described in U.S. Patent, 5,256,775 or U.S. Patent 5,366,878 .
~o [00144] Alkylphosphonothioate oligonucleotides are prepared as described in WO 94/17093 and WO 94/02499 .
[00145] 3'-Deoxy-3'-amino phosphoramidate oligonucleotides are prepared as descn'bed in U.S. Patent 5,476,925 . , [00146] Phosphotriester oligonucleotides are prepared as described in U.S. Patent 5,023,243.
[00147] Borano phosphate oligonucleotides are prepared as described in U.S. Patents 5,130,302 and 5,177,198 .
Example 3 Oligonucleoside Synthesis [00148] llethylenemeQ~ylimino linked oligonucleosides, also ideutifie~l as MMI linked oligonucleosides, methylenedimethylhydrazo linked oligonucleosides, also identified as MDH linked oligonucleosides, and methylenecarbonylamino linked oliganucleosides, also identified as amide-3 linked oligonucleosides, and methyleneaminocarbonyl linked oligonucleosides, also identified as amide-4 linked oligonucleosides, as well as mixed backbone compounds having, for instance, alternating MIvB and P~ or P=S linkages are prepared as described in U.S. Patents 5,378,825;
5,386,023; 5,489,677; 5,602,240; and 5,610,289.

' CA 02495398 2005-02-10 5106'7-55 [00149] Formacetal and thioformacetal linked oligonucleosides are prepared as described in U.S. Patents 5,264,562 and 5,264,564 .
[00150] Ethylene oxide linked oligonucleosides are prepared as described in U.S. Patent 5,223,618 Example 4 PNA Synthesis (00151] Peptide nucleic acids (PNAs) are prepared in accordance with any of the various procedures referred to in Peptide Nucleic Acids (PNA):
Synthesis, Properties and Potential Applications, Bioorganic & Medicinal Chemistry, 1996, 4, 523. They may also be prepared in accordance with U.S. Patents 5,539,082; 5,700,922; and 5,719,262 .
Example 5 Synthesis of Chimeric Oligonucleotides [00152] Chimeric oligonucleotides, oligonucleosides or mixed oligonucleotides/oligonucleosides of the invention caa be of several different types. These include a first type wherein the "gap" segment of linked nucleosides is positioned between 5' and 3' "wing" segments of linked nucleosides and a second "open end" type wherein the "gap" segment is located at either the 3' or the 5' terminus of the oligomeric compound.
Oligonucleotides of the first type are also known in the art as "gapmers" or gapped oligonucleotides. Oligonucleotides of the second type are also known in the art as "hemimers" or "wingmers".
[2'-O-Me]--[2'-deoxy]--[2'-O-Me] Chimeric Phosphorothioate Oligonucleotides [00153] Chimeric oligonucleotides having 2'-O-alkyl phosphorothioate and 2'-deoxy phosphorothioate oligonucleotide segments are synthesized using an Applied Biosystems automated DNA synthesizer Model 380B, as above. Oligonucleotides are synthesized using the automated synthesizer and 2'-deoxy-5'-dimethoxytrityl-3'-O-phosphoramidite for the DNA
portion and 5'-dimethoxytrityl-2'-O-methyl-3'-O-phosphoramidite for 5' and 3' wings. The standard synthesis cycle is modified by increasing the wait step after the delivery of tetrazole and base to 600 s repeated four times for RNA and twice for 2'-O-methyl. The fully protected oligonucleotide is cleaved from the support and the phosphate group is deprotected in 3:1 ammonia/ethanol at room temperature overnight then lyophilized to dryness.
Treatment in methanolic ammonia for 24 hrs at room temperature is then done to deprotect all bases and sample is again lyophilized to dryness. The pellet is resuspended in 1 M TBAF in THF for 24 hrs at room temperature to deprotect the 2' positions. The reaction is then quenched with 1M TEAA
and the sample is then reduced to 1/2 volume by rotovac before being desalted on a G25 size exclusion column. The oligo recovered is then analyzed spectrophotometrically for yield and for purity by capillary electrophoresis and by mass spectrometry.
[2'-O-(2-Methoxyethyl)]--[2'-deoxy]--[2'-O-(Methoxyethyl)] Chimeric Phosphorothioate Oligonucleotides 5106'7-55 [00154] [2'-O-(2: raethoxyethyl)]--[2'-deoxy]-[-2'-O-(methoxyethyl)]
chimeric phosphorothioate oligonucleotides are prepared as per the procedure above for the 2'-O-methyl chimeric oligonucleotide, with the substitution of phorothioate oligonucleotides are prepared as per the procedure above 2'-O-(methoxyethyl) amidites far the 2'-O methyl amidites.
[2'-O-(2-Methoxyethyl)Phosphodiester]-[2'-deoxy PhosphorothioateJ-[2'-O-(~-Methoxyethyl)] Phosphodiester] Chimeric Oligonncleotldes [00155] [2'-O-(2-methoxyethyl phosphodiester]--[2'-deoxy phosphorothioate]-[2'-O-(methcixyethyl) phosphodiester] chimeric oligonucleotides are prepared as per the above procedure for the 2'-O-methyl chimeric oligonucleotide with the substitution of 2'-O-(methoxyethyl) amidites for the 2'-O-methyl amidites, oxidization with iodine to generate the phosphodiester internucleotide linkages within the wing pvrlions of the chimeric structures and sulfurization utilizing 3,H-1,2 benzodithiole-3-one 1,1 dioxide (Beaucage Reagent) to generate the phosphorothioate internucleotide linkages for the center gap.
[00156] Other chimeric oligonucleotides, chimeric oligonucleosides aad mixed chimeric oligonucleotidesloligonucleosides are synthesized according to United States patent 5,623,065 .
lFacample 6 Oligonucleotide Isolation [00157] After cleavage from the controlled pore glass column (Applied Biosystems) and deblocking in concentrated ammonium hydroxide at 55'C
~4 for 18 hours, the oligonucleotides or oligonucleosides are purified by precipitation twice out of 0.5 M NaCI with 2.5 volumes ethanol.
Synthesized oligonucleotides are analyzed by polyacrylamide gel electrophoresis on denaturing gels and judged to be at least 85% full-length material. The relative amounts of phosphorothioate and phosphodiester linkages obtained in synthesis are periodically checked by "P nuclear magnetic resonance spectroscopy, and for some studies oligonucleotides are purified by HPLC, as described by Chiang et al., J. Biol. Chem. 1991, 266, 18162-18171.
Example 7 Oligonucleotide Synthesis - 96 Well Plate Format [00158] Oligonucleotides are synthesized via solid phase P(III) phosphoramidite chemistry on an automated synthesizer capable of assembling 96 sequences simultaneously in a standard 96 well format.
Phosphodiester internucleotide linkages are afforded by oxidation with aqueous iodine. Phosphorothioate internucleotide linkages are generated by sulfurization utilizing 3,H-1,2 benzodithiole-3-one 1,1 dioxide (Beaucage Reagent) in anhydrous acetonitrile. Standard base-protected beta-cyanoethyldiisopropyl phosphoramidites can be purchased from commercial vendors (e.g. PE-Applied Biosystems, Foster City, CA, or Pharmacia, Piscataway, NJ). Non-standard nucleosides are synthesized as per known literature or patented methods. They are utilized as base protected betacyanoethyldiisopropyl phosphoramidites.
7s [00159] 0ligonucleotides are cleaved from support and deprotected with concentrated NH40H at elevated temperature (55-60°C) for 12-16 hours and the released product then dried in vacuo. The dried product is then re-suspended in sterile water to afford a master plate from which all analytical and test plate samples are then diluted utilizing robotic pipettors.
Example 8 Oligonucleotide Analysis - 96 Well Plate Format [00160] The concentration of oligonucleotide in each well is assessed by dilution of samples and UV absorption spectroscopy. The full-length integrity of the individual products is evaluated by capillary electrophoresis (CE) in either the 96 well format (Beckman P/ACETM MDQ) or, for individually prepared samples, on a commercial CE apparatus (e.g., Beckman P/ACETM 5000, ABI 270). Base and backbone composition is confirmed by mass analysis of the compounds utilizing electrospray-mass spectroscopy. All assay test plates are diluted from the master plate using single and mufti-channel robotic pipettors. Plates are judged to be acceptable if at least 85% of the compounds on the plate are at least 85%
full length.
Example 9 Cell culture and oligonucleotide treatment [00161] The effect of antisense compounds on target nucleic acid expression can be tested in any of a variety of cell types provided that the target nucleic acid is present at measurable levels. This can be routinely determined using, for example, PCR or Northern blot analysis. The following 6 cell types are provided for illustrative purposes, but other cell types can be routinely used, provided that the target is expressed in the cell type chosen. This can be readily determined by methods routine in the art, S for example Northern blot analysis, Ribonuclease protection assays, or RT-PCR.
T-24 cells:
[00162] The human transitional cell bladder carcinoma cell line T-24 is obtained from the American Type Culture Collection (ATCC) (Manassas, VA). T-24 cells are routinely cultured in complete McCoy's SA basal media (Gibco/Life Technologies, Gaithersburg, MD) supplemented with 10% fetal calf serum (Gibco/Life Technologies, Gaithersburg, MD), penicillin 100 units per mL, and streptomycin 100 micrograms per mL (Gibco/Life Technologies, Gaithersburg, MD). Cells are routinely passaged by 1 S trypsinization and dilution when they reached 90% confluence. Cells are seeded into 96-well plates (Falcon-Primaria #3872) at a density of 7000 cells/well for use in RT-PCR analysis.
[00163] For Northern blotting or other analysis, cells may be seeded onto 100 mm or other standard tissue culture plates and treated similarly, using appropriate volumes of medium and oligonucleotide.
AS49 cells:
[00164] The human lung carcinoma cell line AS49 can be obtained from the American Type Culture Collection (ATCC) (Manassas, VA). AS49 cells axe routinely cultured in DMEM basal media (GibcolLife Technologies, 2S Gaithersburg, MD) supplemented with 10% fetal calf serum (Gibco/Life Technologies, Gaithersburg, MD), penicillin 100 units per mL, and streptomycin 100 micrograms per mL (Gibco/Life Technologies, Gaithersburg, MD). Cells are routinely passaged by trypsinization and dilution when they reached 90% confluence.
NHDF cells:
[00165] Human neonatal dermal fibroblast (NHDF) can be obtained from the Clonetics Corporation (Walkersville MD). NHDFs are routinely maintained in Fibroblast Growth Medium (Clonetics Corporation, Walkersville MD) supplemented as reconnmended by the supplier. Cells are maintained for up to 10 passages as recommended by the supplier.
HEK cells:
[00166] Human embryonic keratinocytes (HEK) can be obtained from the Clonetics Corporation (Walkersville MD). HEKs are routinely maintained in I~eratinocyte Growth Medium (Clonetics Corporation, Walkersville MD) formulated as recommended by the supplier. Cells are routinely maintained for up to 10 passages as recommended by the supplier.
MCF-7 cells:
[00167] The human breast carcinoma cell line MCF-7 is obtained from the American Type Culture Collection (Manassas, VA). MCF-7 cells are routinely cultured in DMEM low glucose (Gibco/Life Technologies, Gaithersburg, MD) supplemented with 10% fetal calf serum (GibcolLife Technologies, Gaithersburg, MD). Cells are routinely passaged by trypsinization and dilution when they reached 90% confluence. Cells are seeded into 96-well plates (Falcon-Primaria #3872) at a density of 7000 cells/well for use in RT-PCR analysis.

[00168] For Northern blotting or other analyses, cells may be seeded onto 100 mm or other standard tissue culture plates and treated similarly, using appropriate volumes of medium and oligonucleotide.
LA4 cells:
[00169] The mouse lung epithelial cell line LA4 is obtained from the American Type Culture Collection (Manassas, VA). LA4 cells are routinely cultured in F 12K medium (Gibco/Life Technologies, Gaithersburg, MD) supplemented with 15% fetal calf serum (Gibco/Life Technologies, Gaithersburg, MD). Cells are routinely passaged by trypsinization and dilution when they reached 90% confluence. Cells are seeded into 96-well plates (Falcon-Primaria #3872) at a density of 3000-6000 cells/ well for use in RT-PCR analysis.
[00170] For Northern blotting or other analyses, cells may be seeded onto 100 mm or other standard tissue culture plates and treated similarly, using appropriate volumes of medium and oligonucleotide.
Treatment with antisense compounds:
[00171] When cells reached 80% confluence, they are treated with oligonucleotide. For cells grown in 96-well plates, wells are washed once with 200 ~.L OPTI-MEMtm-1 reduced-serum medium (Gibco BRL) and then treated with 130 gL of OPTI-MEMTMTM-1 containing 3.75 ~g/mL
LIPOFECTINTM (Gibco BRL) and the desired concentration of oligonucleotide. After 4-7 hours of treatment, the medium is replaced with fresh medium. Cells are harvested 16-24 hours after oligonucleotide treatment.

[00172] The concentration of oligonucleotide used varies from cell line to cell line. To determine the optimal oligonucleotide concentration for a particular cell line, the cells are treated with a positive control oligonucleotide at a range of concentrations.
Example 10 Analysis of oligonucleotide inhibition of Navl.3 expression [00173] Antisense modulation of Navl.3 expression can be assayed in a variety of ways known in the art. For example, Navl .3 mRNA levels can be quantitated by, e.g., Northern blot analysis, competitive polymerase chain reaction (PCR), or real-time PCR (RT-PCR). Real-time quantitative PCR is presently preferred. RNA analysis can be performed on total cellular RNA
or poly(A)+ mRNA. Methods of RNA isolation are taught in, for example, Ausubel, F~M. et al., Cu~f°ent Protocols ih Molecular Biology, Volume l, pp. 4.1.1-4.2.9 and 4.5.1-4.5.3, John Wiley & Sons, Inc., 1993. Northern blot analysis is routine in the art and is taught in, for example, Ausubel, F.M. et al., Current Protocols in Molecular Biology, Volume 1, pp. 4.2.1-4.2.9, John Wiley ~ Sons, Inc., 1996. Real-time quantitative (PCR) can be conveniently accomplished using the commercially available ABI PRISMTM
7700 Sequence Detection System, available from PE-Applied Biosystems, Foster City, CA and used according to manufacturer's instructions. Prior to quantitative PCR analysis, primer-probe sets specific to the target gene being measured are evaluated for their ability to be "multiplexed" with a GAPDH amplification reaction. In multiplexing, both the target gene and the internal standard gene GAPDH are amplified concurrently in a single so sample. In this analysis, mRNA isolated from untreated cells is serially diluted. Each dilution is amplified in the presence of primer-probe sets specific for GAPDH only, target gene only ("single-plexing"), or both (multiplexing). Following PCR amplification, standard curves of GAPDH
and target mRNA signal as a function of dilution are generated from both the single-plexed and multiplexed samples. If both the slope and correlation coefficient of the GAPDH and target signals generated from the multiplexed samples fall within 10% of their corresponding values generated from the single-plexed samples, the primer-probe set specific for that target is deemed as multiplexable. Other methods of PCR are also known in the art.
[00174] Protein levels of Navl .3 can be quantitated in a variety of ways well known in the art, such as immunoprecipitation, Western blot analysis (immunoblotting), ELISA or fluorescence-activated cell sorting (FACS).
Antibodies directed to Navl .3 can be identified and obtained from a variety of sources, such as the MSRS catalog of antibodies (Aerie Corporation, Birmingham, MI), or can be prepared via conventional antibody generation methods. Methods for preparation of polyclonal antisera are taught in, for example, Ausubel, F.M. et al., Curs°ent Protocols in Molecular Biology, Volume 2, pp. 11.12.1-11.12.9, John Wiley & Sons, Inc., 1997. Preparation of monoclonal antibodies is taught in, for example, Ausubel, F.M. et al., Curf~ent Protocols in Molecular Biology, Volume 2, pp. 11.4.1-11.11.5, John Wiley Sons, Inc., 1997.
[00175] Immunoprecipitation methods are standard in the art and can be found at, for example, Ausubel, F.M. et al., Current Pf°otocols isa Molecular Biology, Volume 2, pp. 10.16.110.16.11, John Wiley & Sons, Inc., 1998.

Western blot (immunoblot) analysis is standard in the art and can be found at, for example, Ausubel, F.M. et al., Current Protocols in Molecular Biology, Volume 2, pp. 10.8.1-10.8.21, John Wiley Sons, Inc., 1997.
Enzyme-linked immunosorbent assays (ELISA) are standard in the art and can be found at, for example, Ausubel, F.M. et al., Curs°ent Protocols ira Molecular Biology, Volume 2, pp. 11.2.1-11.2.22, John Wiley & Sons, Inc., 1991.
Example 11 Poly(A)+ mRNA isolation [00176] Poly(A)+ mRNA is isolated according to Miura et al., Clin.
Che~i., 1996, 42, 1758-1764. Other methods for poly(A)+ mRNA isolation are taught in, for example, Ausubel, F.M. et al., Curre~at Protocols ire Molecular Biology, Volume 1, pp. 4.5.1-4.5.3, John Wiley & Sons, Inc., 1993. Briefly, for cells grown on 96-well plates, growth medium is removed from the cells and each well is washed with 200 ~,L cold PBS. 60~L lysis buffer (10 mM Tris-HCI, pH 7.6, 1 mM EDTA, 0.5 M NaCI, 0.5% NP-40, mM vanadyl-ribonucleoside complex) is added to each well, the plate is gently agitated and then incubated at room temperature for five minutes. 55 20 ~L of lysate is transferred to Oligo d(T) coated 96-well plates (AGCT Inc., Irvine CA). Plates are incubated for 60 minutes at room temperature, washed 3 times with 200 ~,L of wash buffer (10 mM Tris-HC1 pH 7.6, 1 mM EDTA, 0.3 M NaCI). After the final wash, the plate is blotted on paper towels to remove excess wash buffer and then air-dried for 5 minutes. 60 pL
of elution buffer (5 mM Tris-HCl pH 7.6), preheated to 70°C is added to each well, the plate is incubated on a 90°C hot plate for 5 minutes, and the eluate is then transferred to a fresh 96-well plate.
[00177] Cells grown on 100 mm or other standard plates may be treated similarly, using appropriate volumes of all solutions.
Example 12 Total RNA Isolation [00178] Total mRNA is isolated using an RNEASY 96TM kit and buffers purchased from Qiagen Inc. (Valencia CA) following the manufacturer's recommended procedures. Briefly, for cells grown on 96-well plates, growth medium is removed from the cells and each well is washed with 200 ~,L
cold PBS. 100 ~L Buffer RLT is added to each well and the plate vigorously agitated for 20 seconds. 100 ~,L of 70% ethanol is then added to each well and the contents mixed by pipetting three times up and down. The samples are then transferred to the RNEASY 96TM well plate attached to a QIAVACTM
manifold fitted with a waste collection tray and attached to a vacuum source. Vacuum is applied for 15 seconds. 1 mL of Buffer RWl is added to each well of the RNEASY 96TM plate and the vacuum again applied for 15 seconds. 1 mL of Buffer RPE is then added to each well of the RNEASY
96TM plate and the vacuum applied for a period of 15 seconds. The Buffer RPE wash is then repeated and the vacuum is applied for an additional 10 minutes. The plate is then removed from the QIAVACTM manifold and blotted dry on paper towels. The plate is then re-attached to the QIAVACT~~
manifold fitted with a collection tube rack containing 1.2 mL collection tubes. RNA is then diluted by pipetting 60~.L water into each well, incubating one minute, and then applying the vacuum for 30 seconds. The elution step is repeated with an additional 60~L water.
[00179] The repetitive pipetting and elution steps may be automated using a QIAGEN Bio-Robot 9604 (Qiagen, Inc., Valencia CA). Essentially, after lysing of the cells on the culture plate, the plate is transferred to the robot deck where the pipetting, DNase treatment and elution steps are carried out.
Example 13 Real-time Quantitative PCR Analysis of Navl.3 mRNA Levels [00180] Quantitation of Navl .3 mRNA levels is determined by real-time quantitative PCR using the ABI PRISMTM 7700 Sequence Detection System (PE-Applied Biosystems, Foster City, CA) according to manufacturer's instructions. This is a closed-tube, non-gel-based, fluorescence detection system which allows high-throughput quantitation of polymerase chain reaction (PCR) products in real-time. As opposed to standard PCR, in which amplification products are quantitated after the PCR is completed, products in real-time quantitative PCR are quantitated as they accumulate. This is accomplished by including in the PCR reaction an oligonucleotide probe that anneals specifically between the forward and reverse PCR primers, and contains two fluorescent dyes. A reporter dye (e.g., JOE, FAMTM, or VIC, obtained from either Operon Technologies Inc., Alameda, CA or PE-Applied Biosystems, Foster City, CA) is attached to the 5' end of the probe and a quencher dye (e.g., TAMRA, obtained from either Operon Technologies Inc., Alameda, CA or PE-Applied Biosystems, Foster City, CA) is attached to the 3' end of the probe. When the probe and dyes are intact, reporter dye emission is quenched by the proximity of the 3' quencher dye. During amplification, annealing of the probe to the target sequence creates a substrate that can be cleaved by the 5'-exonuclease activity of Taq polymerase. During the extension phase of the PCR
amplification cycle, cleavage of the probe by Taq polymerase releases the reporter dye from the remainder of the probe (and hence from the quencher moiety) and a sequence-specific fluorescent signal is generated. With each cycle, additional reporter dye molecules are cleaved from their respective probes, and the fluorescence intensity is monitored at regular intervals by laser optics built into the ABI PRISMTM 7700 Sequence Detection System.
In each assay, a series of parallel reactions containing serial dilutions of mRNA from untreated control samples generates a standard curve that is used to quantitate the percent inhibition after antisense oligonucleotide treatment of test samples.
[00181] PCR reagents can be obtained from PE-Applied Biosystems, Foster City, CA. RT-PCR reactions are carried out by adding 25 ~.L PCR
cocktail (lx TAQMANTM buffer A, 5.5 MM MgCl2, 300 ~.M each of dATP, dCTP and dGTP, 600 pM of dUTP, 100 nM each of forward primer, reverse primer, and probe, 20 Units RNAse inhibitor, 1.25 Units AMPLITAQ
GOLDTM, and 12.5 Units MuLV reverse transcriptase) to 96 well plates containing 25 ~,L poly(A) mRNA solution. The RT reaction is carried out by incubation for 30 minutes at 48°C. Following a 10 minute incubation at 95°C to activate the AMPLITAQ GOLDTM, 40 cycles of a two-step PCR

protocol are carried out: 95°C for 15 seconds (denaturation) followed by 60°C for 1.5 minutes (annealing/extension).
[00182] Probes and primers to human Navl.3 were designed to hybridize to a human Navl .3 sequence, using published sequence, information (GenBank accession number NM 006922, incorporated herein as Figure 1.
For human Navl .3 the PCR primers were:
forward primer: TTGACTTTGTGGTGGTGATTCTCT SEQ ID N0:9097 reverse primer: CGTAGGATTCGGCCAATCCT SEQ TD N0:9098 and the PCR probe is: FAM- ACCTTGTTCCGAGTGATCCGTCTTGC SEQ ID
NO : 9 0 9 9 -TAMRA where FAMTM (PE-Applied Biosystems, Foster City, CA) is the fluorescent reporter dye) and TAMRA (PE-Applied Biosystems, Foster City, CA) is the quencher dye. For human cyclophilin the PCR
primers were:
forward primer: CCCACCGTGTTCTTCGACAT SEQ ID NO : 910 0 reverse primer: TTTCTGCTGTCTTTGGGACCTT SEQ ID NO : 9101 and the PCR probe is: 5' JOE- CGCGTCTCCTTTGAGCTGTTTGCA SEQ ID
NO : 9102 - TAMRA 3' where JOE (PE-Applied Biosysterns, Foster City, CA) is the fluorescent reporter dye) and TAMRA (PE-Applied Biosystems, Foster City, CA) is the quencher dye.
Example 14 Antisense inhibition of human Navl.3 expression by chimeric phosphorothioate oligonucleotides having 2'-MOE wings and a deoxy gap [00183] In accordance with the present invention, a series of oligonucleotides are designed to target different regions of the human Navl.3 RNA, using published sequences (NM 006922, incorporated herein as Figure 1. The oligonucleotides are shown in Table 1. "Position"
indicates the first (5'-most) nucleotide number on the particular target sequence to which the oligonucleotide binds. The indicated parameters for each oligo were predicted using RNAstructure 3.7 by David H. Mathews, Michael Zuker, and Douglas H. Turner. All compounds in Table 1 are chimeric oligonucleotides ("gapmers") 20 nucleotides in length, composed of a central "gap" region consisting of ten 2'deoxynucleotides, which is I flanked on both sides (5' and 3' directions) by four-nucleotide "wings". The wings are composed of 2'-methoxyethyl (2'-MOE) nucleotides. The internucleoside (backbone) linkages are phosphorothioate (P=S) throughout the oligonucleotide. Cytidine residues in the 2'-MOE wings are 5-methylcytidines. All cytidine residues are 5-methylcytidines.
s7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CTTTTCTTCCTCCGGTTCCT

1969 SEQ ID NO:l -28.5 -28.9 80.8 0 0 -6.6 TTTTCTTCCTCCGGTTCCTC

1968 SEQ ID N0:2 -28 -28.4 80.7 0 0 -6.6 TCTTTTCTTCCTCCGGTTCC

1970 SEQ ID N0:3 -28 -28.4 80.7 0 0 -6.6 CCTCCGGTTCCTCCATTCTT

1961 SEQ ID N0:4 -27.8 -30.5 82.5 -2.1 -0.3 -6.6 TTCTTCCTCCGGTTCCTCCA

1966 SEQ ID N0:5 -27.8 -30.9 84.4 -2.l -0.8 -6.6 TCCTCCGGTTCCTCCATTCT

1962 SEQ ID N0:6 -27.7 -30.8 83.9 -2.1 -0.8 -6.6 TCTTCCTCCGGTTCCTCCAT

1965 SEQ ID N0:7 -27.7 -30.8 83.9 -2.1 -0.8 -6.6 CTTCCTCCGGTTCCTCCATT

1964 SEQ ID N0:8 -27.4 -30.5 82.5 -2.1 -0.8 -6.6 TTTCTTCCTCCGGTTCCTCC

1967 SEQ ID N0:9 -27 -30.3 83.8 -3.3 0 -6.6 TTCCTCCGGTTCCTCCATTC

1963 SEQ ID N0:10 -26.9 -30 82.4 -2.1 -0.8 -6 TCTTCTTTTCTTCCTCCGGT

1973 SEQ ID N0:11 -26.9 -27.3 79.1 0 0 -6.6 CTTCTTTTCTTCCTCCGGTT

1972 SEQ ID N0:12 -26.6 -27 77.6 0 0 -6.6 TTCTTTTCTTCCTCCGGTTC

1971 SEQ TD N0:13 -26.1 -26.5 77.4 0 0 -6.6 CTCCGGTTCCTCCATTCTTT

1960 SEQ ID N0:14 -25.5 -28.6 79.5 -2.1 -0.8 -6.6 ACATCCTTTGCCCGACCTCT

2212 SEQ ID N0:15 -24.3 -30 79.2 -5.7 0 -3 CTGTCTTCTTTTCTTCCTCC

1976 SEQ ID N0:16 -24.1 -26.2 78.6 -2.1 0 0 GGGTCCCCACGGTCTCCCTT

5683 SEQ ID N0:17 -24.1 -34.8 90.5 -9.4 -1.2 -7 GTCTTCTTTTCTTCCTCCGG

1974 SEQ ID N0:18 -24 -27.3 79.1 -3.3 0 -6 TGTCTTCTTTTCTTCCTCCG , 1975 SEQ ID N0:19 -24 -26.1 76.1 -2.1 0 -2.2 TGCCTTTGTTCTGTAGTACT

8639 SEQ ID N0:20 -24 -24.4 73.7 0.3 0 -5.7 TATCTTGTTCCTTTTTGGGC

57g SEQ ID N0:21 -23.8 -24.3 72.7 0.3 -0.2 -3.1 GTTCTGTAGTACTGCTTGGT

8632 SEQ ID N0:22 -23.8 -24.7 76 -0.7 0 -7.1 GATTCCACCAGATCTTCCCT

4035 SEQ ID N0:23 -23.5 -28 77.4 -4.5 0 -5.8 TGGGTCCCCACGGTCTCCCT

5684 SEQ ID N0:24 -23.4 -34.7 89.8 -9.4 -1.9 -9.4 ATTTGCCTTTGTTCTGTAGT

8642 SEQ ID N0:25 -23.4 -23.8 72.3 0.3 0 -3 ATCTTGTTCCTTTTTGGGCT

578 SEQ ID N0:26 -23.3 -25.5 75.4 -1.6 -0.2 -3.8 TCCGGTTCCTCCATTCTTTA

1959 SEQ ID N0:27 -23.3 -27.4 76.9 -3.1 -0.8 -6.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GGTCCCCACGGTCTCCCTTA

5682 SEQ ID N0:28 -23.3 -33.3 87.4 -9.4 -0.3 -3.5 AGGAAGTGGTGTTGGTTTCA

1332 SEQ ID N0:29 -23.2 -23.7 71.8 1.7 -0.2 -4.1 ATGGGTCCCCACGGTCTCCC

5685 SEQ ID N0:30 -23.2 -33.8 87.9 -9.4 -l.1 -9.1 TTGCCTTTGTTCTGTAGTAC

8640 SEQ ID N0:31 -23.2 -23.6 71.9 0.3 0 -4.7 GTAGGAAGTGGTGTTGGTTT

1334 SEQ ID N0:32 -23.1 -23.5 71.8 1.7 0 -1.3 TTCCACCAGATCTTCCCTTT

4033 SEQ ID N0:33 -23.1 -27.6 76.9 -4.5 0 -5.8 TTTGCCTTTGTTCTGTAGTA

8641 SEQ ID N0:34 -23.1 -23.5 71.7 0.3 0 -3 AGTAGGAAGTGGTGTTGGTT

1335 SEQ ID NO:35 -23 -23.4 71.7 1.7 0 -l.3 CTCTGTCTTCTTTTCTTCCT

1978 SEQ ID N0:36 -23 -25.1 76.8 -2.1 0 0 ATTCCACCAGATCTTCCCTT

4034 SEQ TD N0:37 -23 -27.5 76.5 -4.5 0 -5.8 GGGGTAGTGCTCCATGGCCA

2801 SEQ ID N0:38 -22.9 -31.2 86.2 -7.4 -0.5 -9.3 AACATCCTTTGCCCGACCTC

2213 SEQ ID N0:39 -22.7 -28.4 75.1 -5.7 0 -3 TCTGTCTTCTTTTCTTCCTC

1977 SEQ ID N0:40 -22.5 -24.6 76.6 -2.1 0 0 TCTCTGTCTTCTTTTCTTCC

1979 SEQ ID NO:41 -22.5 -24.6 76.6 -2.1 0 0 TCTTGTTCCTTTTTGGGCTT

577 SEQ ID N0:42 -22.4 -25.6 75.8 -2.7 -0.2 -3.8 CATCCTTTGCCCGACCTCTG

2211 SEQ ID N0:43 -22.4 -29.8 78.5 -7.4 0 -3 GATTTTCTCTGACCTCTTTT

6447 SEQ ID N0:44 -22.4 -22.8 69.1 0.6 0 -2.4 GGAAGTGGTGTTGGTTTCAA

1331 SEQ ID N0:45 -22.3 -23 69 1.7 -0.4 -4.1 TAGGAAGTGGTGTTGGTTTC

1333 SEQ ID N0:46 -22.3 -22.7 69.9 1.7 0 -2.6 TGATTTTCTCTGACCTCTTT

6448 SEQ ID N0:47 -22.3 -22.7 68.6 0.6 0 -2.4 GGTTTTGTCAGTAGGCAGTA

7780 SEQ ID N0:48 -22.3 -24.5 75.6 -2.2 0.2 -4 AAGTAGGAAGTGGTGTTGGT

1336 SEQ ID N0:49 -22.2 -22.6 68.7 1.7 0 -1.2 TTATCTTGTTCCTTTTTGGG

580 SEQ ID N0:50 -22.1 -22.6 68.5 0.3 -0.2 -3.l AGATTCCACCAGATCTTCCC

4036 SEQ ID N0:51 -22.1 -27.1 75.8 -4.5 -0.2 -5.8 AGGTTTTGTCAGTAGGCAGT

7781 SEQ ID N0:52 -22.1 -24.8 76.6 -2.2 -0.1 -4 TGTTCTGTAGTACTGCTTGG

8633 SEQ ID N0:53 -22.1 -23.5 71.9 -0.7 0 -7.8 GCCTTTGTTCTGTAGTACTG

8638 SEQ ID N0:54 -22.1 -24.4 73.7 -2.3 0 -6 TTGATTTTCTCTGACCTCTT

6449 SEQ ID N0:55 -22 -22.7 68.6 -0.4 0 -2.4 GCTTTTAATTTGCCTTTGTT

8649 SEQ ID N0:56 -22 -22.4 66.7 0.3 0 -3.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTTTTGTCAGTAGGCAGTAT

7779 SEQ ID N0:57 -21.9 -23.3 72.6 -0.7 -0.1 -4 TGCTTTTAATTTGCCTTTGT

8650 SEQ ID N0:58 -21.9 -22.3 66.3 0.3 0 -3.6 TGGGGTAGTGCTCCATGGCC

2802 SEQ ID N0:59 -21.8 -30.5 85 -7.4 -1.2 -8.1 TTAATTTGCCTTTGTTCTGT

8645 SEQ ID N0:60 -21.6 -22 66.4 0.3 0 -3 GGGTAGTGCTCCATGGCCAT

2800 SEQ ID N0:61 -21.5 -30 83.5 -7.4 -0.4 -10 AATTTGCCTTTGTTCTGTAG

8643 SEQ ID NO:62 -21.5 -21.9 66.3 0.3 0 -2.3 CTCTCTGTCTTCTTTTCTTC

1980 SEQ ID N0:63 -21.4 -23.5 74.7 -2.1 0 0 GTCCCCACGGTCTCCCTTAA

5681 SEQ ID N0:64 -21.4 -31.4 82.4 -9.4 -0.3 -3.5 CAACATCCTTTGCCCGACCT

2214 SEQ ID N0:65 -21.3 -28.7 74.6 -7.4 0 -3 CCATCTGTATTCGAAGGGCA

6075 5EQ ID N0:66 -21.2 -25 70.4 -3.8 0 -7.1 CCATCTGTTTTTTCTGGAGT

6331 SEQ ID N0:67 -21.2 -24 72 -2.8 0 -3.1 TTTGATTTTCTCTGACCTCT

6450 SEQ ID N0:68 -21.2 -22.7 68.6 -1.4 0 -2.4 TAATTTGCCTTTGTTCTGTA

8644 SEQ ID N0:69 -21.2 -21.6 65.5 0.3 0 -3 GCCAGTTCCATGGATCACGA

1029 SEQ ID N0:70 -21.1 -27.4 75.4 -4.9 -0.8 -10.5 ATAGGTTTTGTCAGTAGGCA

7783 SEQ ID N0:71 -21.1 -23.3 71.7 -2.2 0 -4 TTTGTTCTGTAGTACTGCTT

8635 SEQ ID N0:72 -21.1 -22.5 70 -0.7 0 -7.8 ATTATCTTGTTCCTTTTTGG

581 SEQ ID N0:73 -21 -21.4 65.8 0.3 0 -2.9 TTGTTCTGTAGTACTGCTTG

8634 SEQ ID N0:74 -21 -22.4 69.5 -0.7 0 -7.8 TCCTTTGCCCGACCTCTGAA

2209 SEQ ID N0:75 -20.9 -29 76.4 -8.1 0 -3 CCCCACGGTCTCCCTTAACT

5679 SEQ ID N0:76 -20.9 -30.9 79.8 -9.4 -0.3 -3.3 TCCATCTGTATTCGAAGGGC

6076 SEQ ID N0:77 -20.9 -24.7 70.9 -3.8 , 0 -7.1 GCTGTTCGAG'CATCTGCTGA

1806 SEQ ID N0:78 -20.8 -26.9 77.1 -4.5 -1.5 -7.9 TTTGACGCTGTCTTCAGATT

2051 SEQ ID NO:79 -20.6 -22.9 68.1 -0.6 -1.4 -7.6 TTTTGACGCTGTCTTCAGAT

2052 SEQ ID N0:80 -20.6 -22.9 68.1 -0.6 -1.4 -7.6 GATGGGTCCCCACGGTCTCC

5686 SEQ ID N0:81 -20.6 -32.4 86 -10.9 -0.6 -8.8 TCATGTCTTGTTTTATAGGT

6285 SEQ ID N0:82 -20.6 -21 66.3 1.3 0 -4.7 TAGGTTTTGTCAGTAGGCAG

7782 SEQ ID N0:83 -20.6 -23.3 72.1 -2.2 -0.1 -4 TTTTAATTTGCCTTTGTTCT

8647 SEQ ID NO:84 -20.6 -21 64 0.3 0 -3 CTTTTAATTTGCCTTTGTTC

8648 SEQ ID N0:85 -20.6 -21 64 0.3 0 -3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTTTCTCTGACCTCTTTTCC

6445 SEQ ID N0:86 -20.5 -24.6 73.4 -4.1 0 -2.2 TTTAATTTGCCTTTGTTCTG

8646 SEQ ID N0:87 -20.5 -20.9 63.5 0.3 0 -3 TCTCTCTGTCTTCTTTTCTT

1981 SEQ ID N0:88 -20.4 -23.5 74.7 -3.1 0 0 CCCTCTGGGGGAAGTTGTCC

2473 SEQ ID NO:89 -20.4 -29.8 82.2 -7.8 -1.5 -7.6 TCTATAACTTTTGGCTTTCT

3565 SEQ ID N0:90 -20.4 -20.8 64.1 0 0 -3.7 TCCCCACGGTCTCCCTTAAC

5680 SEQ ID N0:91 -20.4 -30.4 79.6 -9.4 -0.3 -3.5 CCATCTTTTCATCCTGCACA

456 SEQ ID N0:92 -20.3 -26.1 74 -5.8 0 -4.8 GAAGTGGTGTTGGTTTCAAA

1330 SEQ ID N0:93 -20.3 -21.1 64 1.7 -0.6 -3.5 AAAGTAGGAAGTGGTGTTGG

1337 SEQ ID N0:94 -20.3 -20.7 63.1 1.7 0 -l.3 AGATAGCCAGCGCCAACATT

4675 SEQ ID N0:95 -20.3 -26.2 71.3 -5.9 0.3 -7.6 TTTCTCTGACCTCTTTTCCT

6444 SEQ ID N0:96 -20.3 -25.4 75 -5.1 0 -2.4 CCGGTTCCTCCATTCTTTAG

1958 SEQ ID N0:97 -20.2 -27 75.5 -5.9 -0.8 -5.4 CAAGATAGCCAGCGCCAACA

4677 SEQ TD N0:98 -20.2 -26.1 69.9 -5.2 -0.5 -7.6 AAGCTGCTTTTAATTTGCCT

8654 SEQ ID N0:99 -20.2 -22.9 66.6 -2.1 0 -8.6 TCCAACATCCTTTGCCCGAC

2216 SEQ ID N0:100 -20.1 -28.2 74.4 -8.1 0 -3 CCTACTCCACTGAAATCTCT

1879 SEQ ID NO:101 -20 -23.7 67.9 -3.7 0 -2.5 GGATTTCTATAACTTTTGGC

3570 SEQ ID N0:102 -20 -20.4 62.5 0 0 -2.8 ATCCAACATCCTTTGCCCGA

2217 SEQ ID N0:103 -19.9 -28 73.8 -8.l 0 -3 ATCACAGCAGTCCCAGATCA

2684 SEQ ID N0:104 -19.9 -26.5 76 -6.6 0 -5.4 CATGTCTTGTTTTATAGGTA

6284 SEQ ID N0:105 -19.9 -20.3 64 1.3 0 -3.5 TGGCCTGCATCTGAGCCATT

1486 SEQ ID N0:106 -19.8 -28.9 79.6 -6.9 -2.2 -7.5 CCTTTGCCCGACCTCTGAAA

2208 SEQ ID N0:107 -19.8 -27.9 72.7 -8.1 0 -2.8 CCAACATCCTTTGCCCGACC

2215 SEQ ID N0:108 -19.8 -29.8 76 -10 0 -3 GGTAGTGCTCCATGGCCATA

2799 SEQ ID N0:109 -19.8 -28.5 80.3 -7.4 -0.4 -10.4 CATGACCAGGAAGAGCAGGA

5450 SEQ ID N0:110 -19.8 -24.2 68.7 -4.4 0 -4.1 AGCCAGTTCCATGGATCACG

1030 SEQ ID N0:111 -19.7 -26.8 74.4 -6 -0.8 -9.7 ATCCTTTGCCCGACCTCTGA

2210 SEQ ID N0:112 -19.7 -29.7 78.8 -10 0 -3 CTATAACTTTTGGCTTTCTA

3564 SEQ~ID N0:113 -19.7 -20.1 62 0 0 -3.7 GATTTCTATAACTTTTGGCT

3569 SEQ ID N0:114 -19.7 -20.1 61.9 0 0 -3.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTCTTGTTTTATAGGTAAGT

6281 SEQ ID N0:115 -19.7 -20.1 64.2 1.3 0 -2.7 AATAGGTTTTGTCAGTAGGC

7784 SEQ TD NO:116 -19.7 -21.9 67.8 -2.2 0 -2.8 CCATTTGAATCCATTGTGCC

1360 SEQ ID N0:117 -19.6 -25 69.7 -5.4 0 -3 CTTGTTTTGCTATTGCGTCT

2179 SEQ TD N0:118 -19.6 -24.1 71.2 -2.9 -1.5 -4 TTCTATAACTTTTGGCTTTC

3566 SEQ ID N0:119 -19.6 -20 62.4 0 0 -3.7 GTGCCATCTTTTCATCCTGC

459 SEQ ID N0:120 -19.5 -27.5 79 -8 0 -3 CTTGTTCCTTTTTGGGCTTC

576 SEQ ID N0:121 -19.5 -25.6 75.8 -5.6 -0.2 -3.8 ATGTTTTCCCAGCAGCACGT

1650 SEQ ID N0:122 -19.5 -27.8 77.3 -8.3 0 -5.4 AGCTGTTCGAGCATCTGCTG

1807 SEQ ID N0:123 -19.5 -26.3 76 -4.5 -2.3 -9.1 CCCACGGTCTCCCTTAACTG

5678 SEQ ID N0:124 -19.5 -28.9 76.4 -9.4 0.2 -3.5 ATCTTTTCATCCTGCACATT

454 SEQ ID N0:125 -19.4 -23.5 69.5 -4.1 0 -4.8 ACATGACCAGGAAGAGCAGG

5451 SEQ ID N0:126 -19.4 -23.8 68 -4.4 0 -5.2 ATCATGTCTTGTTTTATAGG

6286 SEQ ID N0:127 -19.4 -19.8 62.8 l.3 0 -4.7 TTCTCTGACCTCTTTTCCTT

6443 SEQ ID N0:128 -19.4 -25.4 75 -6 0 -2.4 GAGCAGCATAGGCAATATTA

7564 SEQ ID N0:129 -19.4 -21.8 64.8 -1.4 -0.8 -6.2 GCATCTGAGCCATTTCCACA

1480 SEQ ID N0:130 -19.3 -27.1 76 -7.8 0 -3.8 ACCTACTCCACTGAAATCTC

1880 SEQ 2D N0:131 -19.3 -23 66.6 -3.7 0 -2.5 TTTCTATAACTTTTGGCTTT

3567 SEQ ID N0:132 -19.3 -19.7 61.3 0 0 -3.7 GTTTACGTTTCAAAGTGGTT

6147 SEQ ID N0:133. -19.3 -20.9 63.5 -1.6 0 -4.8 TGTTTTCCCAGCAGCACGTA

1649 SEQ ID N0:134 -19.2 -27.5 76.7 -8.3 0 -5.4 TTGACGCTGTCTTCAGATTC

2050 SEQ ID N0:135 -19.2 -23.2 69.4 -2.5 , -1.4 -7.6 TGTTTTGCTATTGCGTCTTG

2177 SEQ ID N0:136 -19.2 -23.2 69 -2.4 -1.5 -4 TCACAGCAGTCCCAGATCAA

2683 SEQ ID N0:137 -19.2 -25.8 73.5 -6.6 0 -5.4 CAGTAGCAGCAAGGTTGTCT

3438 SEQ ID N0:138 -19.2 -24.9 74.5 -5.7 0 -4.6 ATTTCTATAACTTTTGGCTT

3568 SEQ ID N0:139 -19.2 -19.6 60.9 0 0 -3.7 CATCTGTATTCGAAGGGCAT

6074 SEQ ID N0:140 -19.2 -23 66.8 -3.8 0 -7.1 TTCCATCTGTATTCGAAGGG

6077 SEQ ID N0:141 -19.2 -23 67 -3.8 0 -7.1 TGTTTACGTTTCAAAGTGGT

6148 SEQ ID N0:142 -19.2 -20.8 63 -1.6 0 -4.5 CTTGTTTTATAGGTAAGTCA

6279 SEQ ID N0:143 -19.2 -19.6 62.1 1.3 0 -2.3 _ kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTCAATAATCATGTCTTGTT

6293 SEQ ID N0:144 -19.2 -19.6 61.4 1.3 0 -4.7 GTTTGTCAATAATCATGTCT

6297 SEQ ID N0:145 -19.2 -19.6 61.4 1.3 0 -4.7 CATCTGTTTTTTCTGGAGTG

6330 SEQ ID N0:146 -19.2 -22 67.9 -2.8 0 -2.7 ATTTTCTCTGACCTCTTTTC

6446 SEQ ID N0:147 -19.2 -22.6 69.4 -3.4 0 -2.4 GATCTAGGTTTGAGTGTTTG

8096 SEQ ID N0:148 -19.2 -21.4 67.1 -2.2 0 -4.1 TGATCTAGGTTTGAGTGTTT

8097 SEQ ID N0:149 -19.2 -21.4 67.1 -2.2 0 -4.9 CATGGTTTGGCCAGCGACCT

3329 SEQ ID N0:150 -19.1 -29.3 78.5 -8.4 -1.4 -11.4 TGTCAATAATCATGTCTTGT

6294 SEQ ID N0:151 -19.1 -19.5 60.9 1.3 0 -4.7 ATGATCTAGGTTTGAGTGTT

8098 SEQ ID N0:152 -19.1 -21.3 66.7 -2.2 0 -4.9 TGCCATCTTTTCATCCTGCA

458 SEQ ID N0:153 -19 -27 76.5 -8 0 -4.7 TTGTTCCTTTTTGGGCTTCT

575 SEQ ID N0:154 -19 -25.6 75.8 -6.6 0.4 -3.8 AAGTGGTGTTGGTTTCAAAA

1329 SEQ ID NO:155 -19 -19.8 60.5 1.7 -0.6 -3.1 TGTCTTCCATCTGTATTCGA

6081 SEQ ID N0:156 -19 -23.8 70.6 -4.8 0 -4.4 TTTTGATTTTCTCTGACCTC

6451 SEQ ID N0:157 -19 -21.9 66.9 -2.9 0 -2.4 GAAGTTGTTTATCAGGCTAT

7435 SEQ ID N0:158 -19 -21 64.8 -2 0 -5.2 AGAAGTTGTTTATCAGGCTA

7436 SEQ ID N0:159 -19 -21 65.1 -2 0 -5.2 TAGAAGTTGTTTATCAGGCT

7437 SEQ ID N0:160 -19 -21 65.1 -2 0 -5.2 AGAGCAGCATAGGCAATATT

7565 SEQ ID N0:161 -19 -22.1 65.7 -1.4 -1.6 -6.7 CATCTTTTCATCCTGCACAT

455 SEQ ID N0:162 -18.9 -24.1 70.3 -5.2 0 -4.8 GCCATCTTTTCATCCTGCAC

457 SEQ ID N0:163 -18.9 -27.2 77.3 -8.3 0 -4.8 CATTATCTTGTTCCTTTTTG

582 SEQ ID N0:164 -18.9 -20.9 64.3 -2 0 -1.3 CCCAGCAGCACGTAATGTCA

1643 SEQ ID N0:165 -18.9 -27.2 74.2 -8.3 0 -5.4 TCTTGTTTTATAGGTAAGTC

6280 SEQ ID N0:166 -18.9 -19.3 62.3 1.3 0 -2.7 CTTTGCCACTTTGTTCATGG

8276 SEQ ID N0:167 -18.9 -24.5 71.3 -5.6 0.4 -4.7 TCTTTTCATCCTGCACATTT

453 SEQ ID N0:168 -18.8 -23.6 69.9 -4.8 0 -4.8 TAAAGTAGGAAGTGGTGTTG

1338 SEQ ID N0:169 -18.8 -19.2 59.8 1.7 0 -1.3 AAGCTGTTCGAGCATCTGCT

1808 SEQ TD N0:170 -18.8 -25.6 73.7 -4.5 -2.3 -9.6 TTGTTTTGCTATTGCGTCTT

2178 SEQ ID N0:171 -18.8 -23.3 69.5 -2.9 -1.5 -4 TCACGGCTCTTTGCCTTCCA

2571 SEQ ID N0:172 -18.8 -29.4 80.5 -7.9 -2.7 -6.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Tnter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CACAGCAGTCCCAGATCAAG

2682 SEQ ID N0:173 -18.8 -25.4 72.1 -6.6 0 -5.4 GCATGGTTTTGATAGTCTTT

4170 SEQ ID N0:174 -18.8 -22.2 68.2 -3.4 0 -5 AAGATAGCCAGCGCCAACAT

4676 SEQ ID N0:175 -18.8 -25.4 68.9 -5.9 -0.5 -7.6 AGTTGTTTATCAGGCTATAT

7433 SEQ ID N0:176 -18.8 -20.8 65.1 -2 0 -5.2 CCAGTTCCATGGATCACGAA

1028 SEQ ID N0:177 -18.7 -24.9 69 -4.9 -0.1 -10.5 GGCCTGCATCTGAGCCATTT

1485 SEQ ID N0:178 -18.7 -29 80.2 -8.7 -l.5 -7.4 GCTTGTTTTGCTATTGCGTC

2180 SEQ ID N0:179 -18.7 -25 73.6 -4.7 -1.5 -5.3 ACAGCAGTCCCAGATCAAGA

2681 SEQ ID N0:180 -18.7 -25.3 72.3 -6.6 0 -5.4 GGCACATGGTTTGGCCAGCG

3333 SEQ ID N0:181 -18.7 -29.5 79.6 -8.4 -2.4 -9.4 AAGATTCCACCAGATCTTCC

4037 SEQ ID N0:182 -18.7 -24.4 69.9 -4.5 -1.1 -5.8 AGCATGGTTTTGATAGTCTT

4171 SEQ ID N0:183 -18.7 -22.1 68.1 -3.4 0 -5 AGTGGTTGTAATAGGCTCAT

6134 SEQ TD N0:184 -18.7 -22.6 68.7 -3.9 0 -3.7 TCCCAGCAGCACGTAATGTC

1644 5EQ ID N0:185 -18.6 -26.9 74.8 -8.3 0 -4.9 CCTTCTCTTTCTGACTTCCG

2507 SEQ ID N0:186 -18.6 -26.1 74.5 -7.5 0 -2.6 GCCTTCCAGAGGAATCCTCC

2559 SEQ TD N0:187 -18.6 -29.3 80.1 -8.5 -2.2 -11.2 GCTCTTTGCCTTCCAGAGGA

2566 SEQ ID N0:188 -18.6 -28.5 80.9 -8.5 -l.3 -5.6 ACATGGTTTGGCCAGCGACC

3330 SEQ ID N0:189 -18.6 -28.6 77.2 -8.4 -1.4 -10.4 CTTCTTTTTCTGCTGGTTGA

4883 SEQ ID N0:190 -18.6 -23.8 72.1 -5.2 0 -3.7 TAGAGCAGCATAGGCAATAT

7566 SEQ ID N0:191 -18.6 -21.7 64.7 -1.4 -l.6 -6.7 ATAGAGCAGCATAGGCAATA

7567 SEQ ID N0:192 -18.6 -21.7 64.7 -1.4 -1.6 -6.7 GCTTATGCCTCCGCTTCGCT

1 SEQ ID N0:193 -18.5 -31.1 82.6 -11.7 , -0.8 -4.9 GTTTTCCCAGCAGCACGTAA

1648 SEQ ID N0:194 -18.5 -26.8 74.4 -8.3 0 -5.4 CTTTTGACGCTGTCTTCAGA

2053 SEQ ID N0:195 -18.5 -23.8 70.2 -3.8 -1.4 -6.9 ACACCAAGGTGAGGTTTCCT

3117 SEQ ID N0:196 -18.5 -26 74 -6.4 -0.8 -9.6 TCGAAGATTCCACCAGATCT

4040 SEQ ID N0:197 -18.5 -23.7 67.3 -4.5 -0.4 -6 GTTTCTGAGGTTTCTTGGAT

4971 SEQ ID N0:198 -18.5 -23.1 71.1 -4.6 0 -28 ACCTGTCTTCCATCTGTATT

6084 SEQ ID N0:199 -18.5 -25.1 74 -6.6 0 -17 TTACGTTTCAAAGTGGTTGT

6145 SEQ TD N0:200 -18.5 -20.8 63 -1.6 -0.4 -5.6 TGTCTTGTTTTATAGGTAAG

6282 SEQ ID N0:201 -18.5 -18.9 60.7 1.3 0 -24 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATGTCTTGTTTTATAGGTAA

6283 SEQ ID N0:202 -18.5 -18.9 60.4 1.3 0 -2.1 GTTGTTTATCAGGCTATATA

7432 SEQ ID N0:203 -18.5 -20.5 64.2 -2 0 -5.2 GAATAGGTTTTGTCAGTAGG

7785 SEQ ID N0:204 -18.5 -20.7 64.7 -2.2 0 -2.6 TTCTGTAGTACTGCTTGGTG

8631 SEQ ID NO:205 -18.5 -23.5 71.9 -4.5 0 -7.8 TTTTCCCAATAGTCTTGAGT

1612 SEQ ID N0:206 -18.4 -22.8 68.1 -3.9 -0.2 -2.9 CTCTCTCTGTCTTCTTTTCT

1982 5EQ ID N0:207 -18.4 -24.3 76.5 -5.9 0 0 GGCTCTTTGCCTTCCAGAGG

2567 SEQ ID NO:208 -18.4 -29.1 82.2 -8.5 -2.2 -7.7 TCCACCAGATCTTCCCTTTG

4032 SEQ ID N0:209 -18.4 -27.5 76.3 -9.1 0 -5.8 CATGTTACCCGTTGTCATGT

4568 SEQ ID NO:210 -18.4 -25.5 72.4 -6.3 -0.6 -4.7 AACTTCTTTTTCTGCTGGTT

4885 SEQ TD N0:211 -18.4 -22.7 68.9 -4.3 0 -3.7 GTGGTTGTAATAGGCTCATA

6133 SEQ ID N0:212 -18.4 -22.3 67.8 -3.9 0 -3.7 TCACTGTCTTGTATAGGCAC

6600 SEQ ID N0:213 -18.4 -23.3 70.9 -4.2 -0.5 -4 TTCACTGTCTTGTATAGGCA

6601 SEQ ID N0:214 -18.4 -23.2 70.7 -4.1 -0.4 -4 ATCTAGGTTTGAGTGTTTGA

8095 SEQ ID N0:215 -18.4 -21.4 67.1 -3 0 -4 ATTAAAGTAGGAAGTGGTGT

1340 SEQ ID N0:216 -18.3 -19.2 59.9 -0.8 0 -3 CATTAAAGTAGGAAGTGGTG

1341 SEQ ID N0:217 -18.3 -18.7 58.1 0.9 0 -3 TTCCCAGCAGCACGTAATGT

1645 SEQ ID N0:218 -18.3 -26.6 73.5 -8.3 0 -5.4 CTGTTCGAGCATCTGCTGAA

1805 SEQ ID NO:219 -18.3 -24.4 70.3 -4.5 -1.5 -9.5 GTTTTGCTATTGCGTCTTGG

2176 SEQ TD N0:220 -18.3 -24.4 71.8 -5.2 -0.8 -4.2 GTACAGACAATCCCTCCACA

3000 SEQ ID N0:221 -18.3 -25.9 72 -7.6 0 -4.6 TAGCATGGTTTTGATAGTCT

4172 SEQ ID N0:222 -18.3 -21.7 67.1 -3.4 0 -5 CTCAGGGGCTCTGCACTTTC

5809 SEQ ID N0:223 -18.3 -27.8 81.1 -8.5 -0.9 -4.8 GTCTTCCATCTGTATTCGAA

6080 SEQ ID N0:224 -18.3 -23.1 68.4 -4.8 0 -6.8 AGTTTGTCAATAATCATGTC

6298 SEQ ID N0:225 -18.3 -18.7 59.5 1.3 0 -4.7 AATGATCTAGGTTTGAGTGT

8099 SEQ ID N0:226 -18.3 -20.5 63.9 -2.2 0 -4.9 CCATTAAAGTAGGAAGTGGT

1342 SEQ ID N0:227 -18.2 -20.7 62 -2.5 0 -3.3 CACCAAGGTGAGGTTTCCTA

3116 SEQ ID N0:228 -18.2 -25.5 72.8 -6.4 -0.5 -9 TGGATTTCTATAACTTTTGG

3571 SEQ ID N0:229 -18.2 -18.6 58.3 0 0 -2.3 TGCTGTTGCCAAAGGTCTCA

5547 SEQ ID N0:230 -18.2 -25.9 74.1 -6.8 -0.8 -4.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Tntra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CGTTTCAAAGTGGTTGTAAT

6142 SEQ ID N0:231 -18.2 -19.8 60 -1.6 0.2 -3.2 AATAGAGCAGCATAGGCAAT

7568 SEQ ID N0:232 -18.2 -21.3 63.1 -1.4 -1.6 -6.7 TCATTATCTTGTTCCTTTTT

583 SEQ ID N0:233 -18.1 -21.3 66 -3.2 0 -1.9 AGTTCCATGGATCACGAAGA

1026 SEQ ID N0:234 -18.1 -22.8 65.9 -3.4 0 -10.5 CAGCCAGTTCCATGGATCAC

1031 SEQ ID N0:235 -18.1 -26.7 75.6 -7.4 -0.8 -10 CGCTGAGACATTGCCCAGGT

1094 SEQ ID N0:236 -18.1 -28.6 77.2 -10 -0.2 -4.1 TGCATCTGAGCCATTTCCAC

1481 SEQ ID N0:237 -18.1 -26.4 74.7 -7.8 -0.1 -4.8 CAACTGCCTGAGCTTCTTCC

1839 SEQ ID N0:238 -18.1 -27 76 -8.2 -0.4 -5.2 CTACTCCACTGAAATCTCTT

1878 SEQ ID N0:239 -18.1 -21.8 64.6 -3.7 0 -2.5 TATAACTTTTGGCTTTCTAA

3563 SEQ ID N0:240 -18.1 -18.5 58 0 0 -3.7 GATAGCCAGCGCCAACATTA

4674 SEQ ID N0:241 -18.1 -25.9 70.5 -7.1 -0.5 -7.6 GCAGCGTGCGGATCCCCTTT

5385 SEQ ID N0:242 -18.1 -32.2 83.3 -12.4 -1.4 -11.1 AGCAGCGTGCGGATCCCCTT

5386 SEQ ID N0:243 -18.1 -32.1 83.3 -11.8 -2.2 -11.1 TTGTTTACGTTTCAAAGTGG

6149 SEQ ID N0:244 -18.1 -19.7 60.3 -1.6 0 -4.8 TCAATAATCATGTCTTGTTT

6292 SEQ ID N0:245 -18.1 -18.5 58.5 1.3 0 -4.7 TTTGTCAATAATCATGTCTT

6296 SEQ ID N0:246 -18.1 -18.5 58.5 1.3 0 -4.7 AAGTTGTTTATCAGGCTATA

7434 SEQ ID N0:247 -18.1 -20.1 62.8 -2 0 -5.2 ATAGAAGTTGTTTATCAGGC

7438 SEQ ID N0:248 -18.1 -20.1 63 -2 0 -5 CCTTTTTGGGCTTCTTGGCT

570 SEQ ID N0:249 -18 -27.8 79.3 -8.8 -0.9 -4.3 TCCTTTTTGGGCTTCTTGGC

571 SEQ ID N0:250 -18 -27.3 79.1 -8.8 -0.2 -4 TATGTTTTCCCAGCAGCACG

1651 SEQ ID N0:251 -18 -26.3 73.3 -8.3 , 0 -5.4 TACTGTTGCGTCGCTCTCCA

2322 SEQ ID N0:252 -18 -28.2 78.4 -9.6 -0.3 -6.9 ACCTTCTCTTTCTGACTTCC

2508 SEQ ID N0:253 -18 -25.5 75.3 -7.5 0 -2.6 GCAGTCCCAGATCAAGAACA

2678 SEQ ID N0:254 -18 -24.6 69.7 -6.6 0 -5.4 GTAGTGCTCCATGGCCATAA

2798 SEQ ID N0:255 -18 -26.6 75 -7.4 0.3 -10.4 TGGCCAACACCAAGGTGAGG

3123 SEQ ID N0:256 -18 -26.3 71.8 -6.7 -1 -11.2 CAGGAAGGAGTGGAAGAAGT

3254 SEQ ID N0:257 -18 -21 62.8 -3 0 -1.6 GCAAGATAGCCAGCGCCAAC

4678 SEQ ID N0:258 -18 -27.2 72.8 -8.5 -0.5 -7.6 GGTTTCTGAGGTTTCTTGGA

4972 SEQ ID N0:259 -18 -24.3 74 -6.3 0 -2.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAGTGGTTGTAATAGGCTCA

6135 SEQ ID N0:260 -18 -21.9 66.4 -3.9 0 -3.7 TTGTCAATAATCATGTCTTG

6295 SEQ ID N0:261 -18 -18.4 58.1 1.3 p -4.7 TTTTTCTGGTTTGTCTTTCT

6416 SEQ ID N0:262 -18 -22.3 70.2 -4.3 0 -1.5 GTCATGGGGTAGTGCTCCAT

2806 SEQ ID N0:263 -17.9 -27.8 80.9 -7.9 -2 -8.9 AGTCATGGGGTAGTGCTCCA

2807 SEQ ID N0:264 -17.9 -27.8 81.4 -8.9 -0.9 -7.2 GCTGTTGCCAAAGGTCTCAA

5546 SEQ ID N0:265 -17.9 -25.2 71.8 -6.8 -0.1 -3.7 TCTCTGACCTCTTTTCCTTT

6442 SEQ ID N0:266 -17.9 -25.4 75 -7.5 0 -2.4 TTTGGTGTAGTTACAATGTT

6756 SEQ ID N0:267 -17.9 -20 62.6 -2.1 0.3 -4.4 GATTTTCCCAATAGTCTTGA

1614 SEQ ID N0:268 -17.8 -22.2 65.9 -3.9 -0.2 -2.7 AACTGCCTGAGCTTCTTCCT

1838 SEQ ID N0:269 -17.8 -27.2 76.8 -8.2 -1.1 -5.2 CACCTACTCCACTGAAATCT

1881 SEQ ID N0:270 -17.8 -23.3 66.2 -5.5 0 -2.5 TCTTTTGACGCTGTCTTCAG

2054 SEQ ID N0:271 -17.8 -23.6 70.5 -4.7 -1 -7.6 CTTCTCTTTCTGACTTCCGT

2506 SEQ ID N0:272 -17.8 -25.3 74.3 -7.5 0 -2.6 CAGCAGTCCCAGATCAAGAA

2680 SEQ ID N0:273 -17.8 -24.4 69.4 -6.6 0 -5.4 CCACAAGGTTTCCAATGACC

3375 SEQ ID N0:274 -17.8 -24.7 68.4 -6.2 -0.4 -4.7 CTAGCATGGTTTTGATAGTC

4173 SEQ ID NO:275 -17.8 -21.7 67.1 -3.4 -0.1 -5 ACATGTTACCCGTTGTCATG

4569 SEQ ID N0:276 -17.8 -24.5 69.7 -5.4 -1.2 -7.l CAATAATCATGTCTTGTTTT

6291 SEQ ID NO:277 -17.8 -18.2 57.5 1.3 0 -4.7 CCCATCTGTTTTTTCTGGAG

6332 SEQ ID N0:278 -17.8 -24.8 72.3 -7 0 -3.5 GTTCCATGGATCACGAAGAA

1025 SEQ ID N0:279 -17.7 -22.1 63.6 -3.4 0 -10 AGATTTTCCCAATAGTCTTG

1615 SEQ ID N0:280 -17.7 -21.6 64.8 -3.9 0 -2.8 ACCAAGGTGAGGTTTCCTAG

3115 SEQ ID NO:281 -17.7 -24.8 72 -6.4 -0.5 -6.8 ATAACTTTTGGCTTTCTAAA

3562 SEQ ID N0:282 -17.7 -18.1 56.6 0 0 -3.8 TTCGAAGATTCCACCAGATC

4041 SEQ ID N0:283 -17.7 -22.9 65.8 -4.5 -0.4 -6.2 ATGTTACCCGTTGTCATGTT

4567 SEQ ID N0:284 -17.7 -24.9 71.7 -7.2 0 -4.7 CTTTTTCTGCTGGTTGAAGT

4880 SEQ ID N0:285 -17.7 -22.9 69.2 -5.2 0 -3.7 ACTCAGGGGCTCTGCACTTT

5810 SEQ ID N0:286 -17.7 -27.6 79.9 -8.9 -0.9 -4.8 AACCTGTCTTCCATCTGTAT

6085 SEQ ID N0:287 -17.7 -24.3 71.1 -6.6 0 -2.1 ACGTTTCAAAGTGGTTGTAA

6143 SEQ ID N0:288 -17.7 -20 60.6 -1.6 -0.4 -5.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTAAAGTAGGAAGTGGTGTT

1339 SEQ ID N0:289 -17.6 -19.3 60.3 -1.7 0 -2 CATTTGAATCCATTGTGCCA

1359 SEQ ID N0:290 -17.6 -23.7 67.3 -6.1 0 -3 TAAGCTGTTCGAGCATCTGC

1809 SEQ ID NO:291 -17.6 -24.4 71.1 -4.5 -2.3 -7.6 TTTTGCTATTGCGTCTTGGG

2175 SEQ ID N0:292 -17.6 -24.4 71 -5.2 -l.5 -5 ACTGTTGCGTCGCTCTCCAT

2321 SEQ ID N0:293 -17.6 -28.5 79 -10.3 -0.3 -6.9 AAACTTCTTTTTCTGCTGGT

4886 SEQ ID N0:294 -17.6 -21.9 66.2 -4.3 0 -3.7 CATCCTCACTCAGGGGCTCT

5817 SEQ ID N0:295 -17.6 -28.9 82.5 -10.4 -0.8 -7.6 CTGTATTCGAAGGGCATCCA

6071 SEQ ID N0:296 -17.6 -25 70.4 -6.2 -1.1 -8 TTTACGTTTCAAAGTGGTTG

6146 SEQ ID N0:297 -17.6 -19.7 60.3 -1.6 -0.1 -5 TTGTTTTATAGGTAAGTCAA

6278 SEQ ID N0:298 -17.6 -18 57.8 1.3 0 -2.4 TAGTTTGTCAATAATCATGT

6299 SEQ ID N0:299 -17.6 -18 57.5 1.3 0 -4.7 GTGTTTGGTGTAGTTACAAT

6759 SEQ 2D NO:300 -17.6 -21.1 65.6 -3 -0.2 -4.6 CATCTGAGCCATTTCCACAG

1479 SEQ ID N0:301 -17.5 -25.3 72 -7.8 0 -3.7 CTGGCCTGCATCTGAGCCAT

1487 SEQ TD N0:302 -17.5 -29.7 81.1 -9.9 -2.3 -7.5 TTTGCTATTGCGTCTTGGGG

2174 SEQ ID N0:303 -17.5 -25.5 73.3 -6.4 -1.5 -5 CTCACGGCTCTTTGCCTTCC

2572 SEQ ID N0:304 -17.5 -29.6 81.4 -9.9 -2.2 -5.7 ACAGACAATCCCTCCACATT

2998 SEQ ID NO:305 -17.5 -25.1 69.8 -7.6 0 -2.1 ATGATGGCCAACACCAAGGT

3127 SEQ ID N0:306 -17.5 -25.1 69 -6.7 -0.8 -6.3 ACGATGATGGCCAACACCAA

3130 SEQ ID N0:307 -17.5 -24.3 65.5 -5.9 -0.8 -6.3 TCAGTAGCAGCAAGGTTGTC

3439 SEQ ID N0:308 -17.5 -24.4 74.2 -6.9 0 -5.4 TTCCCATCTCTAAGATAATT

3655 SEQ ID N0:309 -17.5 -20.5 61.5 -2.4 -0.3 -4.3 GTTCACCTTCTCGGGGTAGA

3912 SEQ ID N0:310 -17.5 -27.2 78.3 -8.7 -0.9 -4.9 TTTTCGAAGATTCCACCAGA

4043 SEQ TD NO:3ll -17.5 -22.7 65.1 -4.5 -0.4 -6.8 TAGCCAGCGCCAACATTATC

4672 SEQ ID N0:312 -17.5 -25.7 70.8 -7.5 -0.5 -7.6 TCTGCTGGTTGAAGTTATCT

4875 SEQ ID N0:313 -17.5 -22.7 69.1 -5.2 0 -3.7 AACATGACCAGGAAGAGCAG

5452 SEQ TD N0:314 -17.5 -219 63.4 -4.4 0 -5.2 ATGCTGTTGCCAAAGGTCTC

5548 SEQ ID N0:315 -17.5 -25.2 72.9 -6.8 -0.8 -4.4 CCACGGTCTCCCTTAACTGA

5677 SEQ ID N0:316 -17.5 -27.5 74.3 -9.4 -0.3 -3.5 AATCATGTCTTGTTTTATAG

6287 SEQ ID N0:317 -17.5 -17.9 57.8 1.3 0 -47 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Position oligo bindingformationDuplexstructureoligo oligo ATAATCATGTCTTGTTTTAT

6289 SEQ ID N0:318 -17.5 -17.9 57.6 1.3 0 -4.1 AAATAGAGCAGCATAGGCAA

7569 SEQ ID N0:319 -17.5 -20.6 61.1 -1.4 -1.6 -6.7 AAAATAGAGCAGCATAGGCA

7570 SEQ ID N0:320 -17.5 -20.6 61.1 -1.4 -1.6 -6.7 GTAAAATAGAGCAGCATAGG

7572 SEQ ID N0:321 -17.5 -l9 58.3 -1.4 0 -5.4 ATTGCCTTTCCTTTATTCAT

748 SEQ ID N0:322 -17.4 -23.4 68.9 -6 0 -3 GCAACTGCCTGAGCTTCTTC

1840 SEQ ID N0:323 -17.4 -26.8 76.8 -8.2 -1.1 -5.2 GATCCAACATCCTTTGCCCG

2218 SEQ ID N0:324 -17.4 -28 73.8 -10.6 0 -3.3 TTACTGTTGCGTCGCTCTCC

2323 SEQ ID N0:325 -17.4 -27.6 77.8 -9.6 -0.3 -6.9 CAGACAATCCCTCCACATTT

2997 SEQ ID N0:326 -17.4 -25 69.6 -7.6 0 -2.1 CAGTACAGACAATCCCTCCA

3002 SEQ ID N0:327 -17.4 -25.7 71.8 -7.6 -0.4 -5.3 TTTCGAAGATTCCACCAGAT

4042 SEQ ID N0:328 -17.4 -22.6 64.7 -4.5 -0.4 -6.8 TCTAGCATGGTTTTGATAGT

4174 SEQ ID N0:329 -17.4 -21.7 67.1 -3.4 -0.7 -4.3 GCTGAAGTTTAACATCTCGT

4758 SEQ ID N0:330 -17.4 -21.6 64.4 -4.2 0 -3.6 GGCTGAAGTTTAACATCTCG

4759 SEQ ID N0:331 -17.4 -21.6 63.8 -4.2 0 -4.6 CACTCAGGGGCTCTGCACTT

5811 SEQ ID N0:332 -17.4 -28.2 80.5 -9.8 -0.9 -4.8 CCCTGCTTCACAGAGTTGCA

6637 SEQ ID N0:333 -17.4 -28.1 78.8 -9.4 -1.2 -7 TGGTGTAGTTACAATGTTCA

6754 SEQ ID N0:334 -17.4 -20.9 64.7 -3 -0.2 -4.6 AGCAGCATAGGCAATATTAA

7563 SEQ ID N0:335 -17.4 -20.5 61.4 -1.4 -1.6 -6.7 TAAAATAGAGCAGCATAGGC

7571 SEQ ID N0:336 -17.4 -19.6 59.4 -l.4 -0.5 -5.4 GGTTATATTGAGGCAACCAT

8130 SEQ ID N0:337 -17.4 -22.5 65.7 -3.7 -1.3 -5.6 AGGTATCTCATCCCTGTCAA

267 SEQ ID N0:338 -17.3 -25.4 74.1 -8.1 0 -3.1 GCTGAGACATTGCCCAGGTC

1093 SEQ ID N0:339 -17.3 -28.2 79.3 -10.4 -0.2 -4.4 GCCATTAAAGTAGGAAGTGG

1343 SEQ ID N0:340 -17.3 -21.3 63 -4 0 -3.7 TTTTCCCAGCAGCACGTAAT

1647 SEQ 2D N0:341 -17.3 -25.6 71.2 -8.3 0 -5.4 AGCAGTCCCAGATCAAGAAC

2679 SEQ ID N0:342 -17.3 -23.9 68.9 -6.6 0 -5.4 ACCAAGCTCCATTAAACTGA

2972 SEQ ID N0:343 -17.3 -22.1 63.1 -4.8 0 -5 CAATCAGGAAGGAGTGGAAG

3258 SEQ ID N0:344 -17.3 -20.3 60.9 -3 0 -3.9 CGAAGATTCCACCAGATCTT

4039 SEQ ID N0:345 -17.3 -23.4 66.2 -4.5 -1.5 -5.8 AGCCAGCGCCAACATTATCA

4671 SEQ ID N0:346 -17.3 -26.7 72.4 -8.7 -0.5 -6.8 kcal/

mol kcal/moldeg kcal/mo1kcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTGTTTATCAGGCTATATAT

7431 SEQ ID N0:347 -17.3 -19.3 60.9 -2 0 -5.2 CAGGATACATCCCTGTTGGA

7500 SEQ ID N0:348 -17.3 -25.5 72.3 -6.7 -1.4 -6.9 TTGAGTAAAATAGAGCAGCA

7576 SEQ ID N0:349 -17.3 -18.8 57.9 -1.4 0 -5.4 AGAATAGGTTTTGTCAGTAG

7786 SEQ ID N0:350 -17.3 -19.5 62.1 -2.2 0 -2.7 TTGCCACTTTGTTCATGGCT

8274 5EQ ID N0:351 -17.3 -26.2 75.3 -5.6 -3.3 -9.1 TTTGCCACTTTGTTCATGGC

8275 SEQ TD N0:352 -17.3 -25.4 73.7 -5.6 -2.5 -8.1 ATTTTCCCAATAGTCTTGAG

1613 SEQ ID N0:353 -17.2 -21.6 64.8 -3.9 -0.2 -2.7 TTTCCCAGCAGCACGTAATG

1646 SEQ ID N0:354 -17.2 -25.5 70.7 -8.3 0 -5.4 AACCCACCTACTCCACTGAA

1885 SEQ ID N0:355 -17.2 -26.2 70.3 -9 0 -1.7 ATGTGCTGTGTTCATCATCA

2253 SEQ ID N0:356 -17.2 -23.7 71.9 -6.5 0 -3.6 GATGGCCAACACCAAGGTGA

3125 SEQ ID N0:357 -17.2 -25.7 70.3 -6.7 -0.8 -11.7 TGATGGCCAACACCAAGGTG

3126 SEQ ID N0:358 -17.2 -25.1 68.9 -6.7 -0.8 -9.8 GATGATGGCCAACACCAAGG

3128 SEQ TD N0:359 -17.2 -24.5 67.3 -6.7 -0.3 -6.3 TCAGGAAGGAGTGGAAGAAG

3255 SEQ ID N0:360 -17.2 -20.2 61.2 -3 0 -2.4 ATCAGGAAGGAGTGGAAGAA

3256 SEQ ID N0:361 -17.2 -20.2 60.9 -3 0 -2.9 AATCAGGAAGGAGTGGAAGA

3257 SEQ ID N0:362 -17.2 -20.2 60.9 -3 0 -2.9 AGGTTGTCTGAGCTAAATGA

3427 SEQ ID N0:363 -17.2 -21.2 64.4 -4 0 -5.1 ATTCCCATCTCTAAGATAAT

3656 SEQ ID N0:364 -17.2 -20.4 61.2 -2.5 -0.5 -3.9 CATGGTTTTGATAGTCTTTC

4169 SEQ ID N0:365 -17.2 -20.8 65.3 -3.6 0 -3.7 ATTCTAGCATGGTTTTGATA

4176 SEQ ID N0:366 -17.2 -20.6 63.7 -3.4 0 -5 TATTCTAGCATGGTTTTGAT

4177 SEQ ID N0:367 -17.2 -20.6 63.7 -3.4 0 -5 GAGCAGCGTGCGGATCCCCT

5387 SEQ ID N0:368 -17.2 -32.6 84.2 -13.2 -2.2 -11.1 TCAGGGGCTCTGCACTTTCT

5808 SEQ ID N0:369 -17.2 -27.8 81.1 -9.6 -0.9 -4.8 TAATCATGTCTTGTTTTATA

6288 SEQ ID N0:370 -17.2 -17.6 57 1.3 0 -4.7 TGAGTAAAATAGAGCAGCAT

7575 SEQ ID N0:371 -17.2 -18.7 57.6 -1.4 0 -5.4 TTGAGGCAACCATAGTTGGT

8123 SEQ TD N0:372 -17.2 -24 69.7 -4.4 -2.4 -7.5 TGTTCCTTTTTGGGCTTCTT

574 SEQ ID N0:373 -17.1 -25.6 75.8 -8 -0.2 -3.8 AGTGGTGTTGGTTTCAAAAG

1328 SEQ ID N0:374 -17.1 -20.5 62.9 -2.6 -0.6 -3.1 TACAGACAATCCCTCCACAT

2999 SEQ ID N0:375 -17.1 -24.7 68.9 -7.6 0 -2.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGGCACATGGTTTGGCCAGC

3334 SEQ ID N0:376 -17.1 -28.7 80.4 -8.4 -3.2 -9.6 ATAGCCAGCGCCAACATTAT

4673 SEQ ID N0:377 -17.1 -25.3 69.3 -7.5 -0.5 -7.6 CAAACTTCTTTTTCTGCTGG

4887 SEQ ID N0:378 -17,1 -21.4 64.2 -4.3 0 -3.7 GTTGCCAAAGGTCTCAAAGT

5543 SEQ ID N0:379 -17.1 -23 67 -5.9 0 -4 AGAGGAGGATCCAGGGCAGC

5923 SEQ ID N0:380 -17.1 -28 79.9 -8.3 -2.6 -9 TGGACAGAGCAGGTTGAATT

7648 SEQ ID N0:381 -17.1 -22.3 66.1 -5.2 0 -4.1 TTTGAATCCATTGTGCCATT

1357 SEQ ID N0:382 -17 -23.1 66.6 -6.1 0 -3.1 AAGATTTTCCCAATAGTCTT

1616 SEQ ID N0:383 -17 -20.9 62.8 -3.9 0 -3.5 CCTGTTGCTTTTTAAGCTGT

1821 SEQ ID N0:384 -17 -24.2 71.1 -4.6 -2.6 -7.1 TTCCTGTTGCTTTTTAAGCT

1823 SEQ ID N0:385 -17 -23.5 69.9 -3.9 -2.6 -7.1 CTCTTTCTGACTTCCGTTTC

2503 SEQ ID N0:386 -17 -24.5 72.6 -7.5 0 -2.7 TCTCTTTCTGACTTCCGTTT

2504 SEQ ID N0:387 -17 -24.5 72.6 -7.5 0 -2.6 TTCTCTTTCTGACTTCCGTT

2505 SEQ ID N0:388 -17 -24.5 72.6 -7.5 0 -2.6 TTGCCTTCCAGAGGAATCCT

2561 SEQ ID N0:389 -17 -27 75.1 -8.5 -1.3 -9.8 CAGTCATGGGGTAGTGCTCC

2808 SEQ ID N0:390 -17 -27.8 81.4 -10.3 -0.1 -6.7 AGGAAGGAGTGGAAGAAGTC

3253 SEQ ID N0:391 -17 -20.7 63.1 -3.7 0 -1.8 ATGGATTTCTATAACTTTTG

3572 SEQ ID N0:392 -17 -17.4 55.7 0 0 -2.3 TGTTCACCTTCTCGGGGTAG

3913 SEQ ID N0:393 -17 -26.6 76.8 -8.7 -0.7 -4.2 ATATTCTAGCATGGTTTTGA

4178 SEQ ID N0:394 -17 -20.6 63.7 -3.6 0 -5 AACATGTTACCCGTTGTCAT

4570 SEQ ID N0:395 -17 -23.8 67.6, -6.3 0 -8.1 TTCTTTTTCTGCTGGTTGAA

4882 SEQ ID N0:396 -17 -22.2 67.5 -5.2 0 -3.7 TCTGAGGTTTCTTGGATCCA

4968 SEQ ID N0:397 -17 -24.8 73.6 -6.9 O.l -9.7 TCAGACGTAGGATTCGGCCA

5355 SEQ ID N0:398 -17 -26.4 73.1 -8.8 -0.3 -7.2 AGATGGGTCCCCACGGTCTC

5687 SEQ ID N0:399 -17 -30.4 83 -12.5 -0.6 -8.8 CTGTCTTCCATCTGTATTCG

6082 SEQ ID N0:400 -17 -24.1 71.3 -7.1 0 -2.4 AAACCTGTCTTCCATCTGTA

6086 SEQ ID N0:401 -17 -23.6 68.8 -6.6 0 -2.1 TAAACCTGTCTTCCATCTGT

6087 SEQ ID N0:402 -17 -23.6 68.8 -6.6 0 -2 CAGGGTTCTTGTATACTGTT

6931 SEQ ID N0:403 -17 -22.8 69.9 -5.8 0 -6.5 ATGGACAGAGCAGGTTGAAT

7649 SEQ ID N0:404 -17 -22.2 65.8 -5.2 0 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATATTGAGGCAACCATAGTT

8126 SEQ ID N0:405 -17 -21.3 63.4 -3.7 -0.3 -3.7 CCACTTTGTTCATGGCTAAA

8271 SEQ ID N0:406 -17 -22.6 65.6 -5.6 0 -4.7 CATGCACTAGTTTGTTGTAA

8608 SEQ ID N0:407 -17 -20.9 63.9 -3.9 0 -5.5 ATTTGAATCCATTGTGCCAT

1358 5EQ ID N0:408 -16.9 -23 66.2 -6.1 0 -3.1 GTCCCATTTGAATCCATTGT

1363 SEQ ID N0:409 -16.9 -24.8 70.5 -7.9 0 -2.2 TCGACCAGCCTTCACACAGA

1523 SEQ ID N0:410 -16.9 -27 74.2 -10.1 0 -4.2 TCCTGTTGCTTTTTAAGCTG

1822 SEQ ID N0:411 -16.9 -23.4 69.4 -3.9 -2.6 -7.1 TGCCTTCCAGAGGAATCCTC

2560 SEQ ID N0:412 -16.9 -27.3 76.4 -8.5 -1.8 -10.8 GTGCTCCATGGCCATAAAGA

2795 SEQ ID N0:413 -16.9 -25.6 71.2 -7.4 -0.4 -10.4 AGTACAGACAATCCCTCCAC

3001 SEQ ID N0:414 -16.9 -25.2 71.2 -7.6 -0.4 -5.3 GCACATGGTTTGGCCAGCGA

3332 SEQ ID N0:415 -16.9 -28.9 78.4 -10.4 -l.5 -8.8 TTTAATACACCCTTCAGTAA

3977 SEQ ID N0:416 -16.9 -20.3 60.7 -3.4 0 -3.6 TTTTTCTGCTGGTTGAAGTT

4879 SEQ ID N0:417 -16.9 -22.1 67.5 -5.2 0 -3.7 TCTTTTTCTGCTGGTTGAAG

4881 SEQ ID N0:418 -16.9 -22.1 67.4 -5.2 0 -4 TGTTGCCAAAGGTCTCAAAG

5544 SEQ ID N0:419 -16.9 -21.8 63.8 -4.9 0 -4 CGTTGATACCCTGCTTCACA

6645 SEQ ID N0:420 -16.9 -26.3 72.6 -9.4 0 -3.6 GGTGTTTGGTGTAGTTACAA

6760 SEQ ID N0:421 -16.9 -22.3 68.5 -4.9 -0.2 -4.6 GTTTATCAGGCTATATATAT

7429 SEQ ID N0:422 -16.9 -18.9 60 -2 0 -5 TGTTTATCAGGCTATATATA

7430 SEQ ID N0:423 -16.9 -18.9 59.9 -2 0 -4.5 GAGTAAAATAGAGCAGCATA

7574 SEQ ID N0:424 -16.9 -18.4 57.1 -1.4 0 -5.4 TTTTGTCAGTAGGCAGTATC

7778 SEQ ID N0:425 -16.9 -22.5 70.6 -5.l -0.1 -4 GCCACTTTGTTCATGGCTAA

8272 SEQ TD N0:426 -16.9 -25.1 72 -5.6 -2.6 -8.3 TGCCACTTTGTTCATGGCTA

8273 SEQ ID N0:427 -16.9 -25.8 74.3 -5.6 -3.3 -9.1 ATCTCATTTATTCTTACAAT

347 SEQ TD N0:428 -16.8 -17.7 56.8 -0.7 0 -1.6 ACTGCCTGAGCTTCTTCCTG

1837 SEQ TD N0:429 -16.8 -27.9 79.2 -9.9 -1.1 -5.2 CCAAACTTCTTTTTCTGCTG

4888 SEQ ID N0:430 -16.8 -22.2 65.3 -5.4 0 -3.6 CAGGAAGAGCAGGAGGCCGA

5444 SEQ ID N0:431 -16.8 -27.1 74.1 -9.4 -0.8 -7.7 AAACATGACCAGGAAGAGCA

5453 SEQ ID N0:432 -16.8 -21.2 61.3 -4.4 0 -5.2 AATAATCATGTCTTGTTTTA

6290 SEQ TD N0:433 -16.8 -17.2 55.6 1.3 0 -4.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CACTGTCTTGTATAGGCACT

6599 SEQ ID N0:434 -16.8 -23.8 71.3 -6.3 -0.5 -4 CTTTTCATCCTGCACATTTA

452 SEQ ID N0:435 -16.7 -22.9 67.7 -6.2 0 -4.8 AATTGCCTTTCCTTTATTCA

749 SEQ ID N0:436 -16.7 -22.7 66.6 -6 0 -3 CCATGGATCACGAAGAAACG

1022 SEQ ID N0:437 -16.7 -20.7 58.4 -3.4 0 -8.5 CCAGCCAGTTCCATGGATCA

1032 SEQ ID N0:438 -16.7 -28.5 78.6 -10.6 -0.8 -10 GGTGTTGGTTTCAAAAGCAG

1325 SEQ ID N0:439 -16.7 -21.8 65.2 -4.3 -0.6 -0.8 ACTGGCCTGCATCTGAGCCA

1488 SEQ ID N0:440 -16.7 -29.9 81.8 -ll -2.2 -7.5 TTTAAGCTGTTCGAGCATCT

1811 SEQ ID N0:441 -16.7 -22.8 67.6 -4.5 -1.5 -7.4 CGGTTCCTCCATTCTTTAGC

1957 SEQ ID N0:442 -16.7 -26.8 76.3 -9.2 -0.8 -3.6 CACGGCTCTTTGCCTTCCAG

2570 SEQ ID NO:443 -16.7 -29 79.1 -9.6 -2.7 -6.2 TCCATGGCCATAAAGAGGGT

2791 SEQ ID N0:444 -16.7 -25.3 70.7 -7.4 0 -10.4 ATCAGTAGCAGCAAGGTTGT

3440 SEQ ID N0:445 -16.7 -24 72.3 -7.3 0 -5.4 TTCCATTCCCATCTCTAAGA

3660 SEQ ID N0:446 -16.7 -24.6 70.6 -7.9 0 -2.8 GCTTGTTCACCTTCTCGGGG

3916 SEQ ID N0:447 -16.7 -28.5 80.3 -11.3 -0.2 -3.4 TTCTGCTGGTTGAAGTTATC

4876 SEQ ID N0:448 -16.7 -21.9 67.4 -5.2 0 -3.7 TTTCTGAGGTTTCTTGGATC

4970 SEQ ID N0:449 -16.7 -22.3 69.2 -5.6 0 -4.7 TGTATTCGAAGGGCATCCAT

6070 SEQ ID N0:450 -16.7 -24.1 68.5 -6.2 -1.1 -8.2 ATTGAGGCAACCATAGTTGG

8124 SEQ TD N0:451 -16.7 -22.8 66.4 -3.7 -2.4 -6.8 GTTATATTGAGGCAACCATA

8129 SEQ ID N0:452 -16.7 -21 62.6 -3.7 -0.3 -4 AGGTTATATTGAGGCAACCA ' 8131 SEQ ID N0:453 -16.7 -22.5 65.9 -3.7 -2.1 -6.3 TCATGCACTAGTTTGTTGTA

8609 SEQ ID N0:454 -16.7 -22 67.9 -5.3 0 -5.7 GGTATCTCATCCCTGTCAAA

266 SEQ ID N0:455 -16.6 -24.7 71.4 -8.1 0 -2.4 TTGAATCCATTGTGCCATTA

1356 SEQ ID N0:456 -16.6 -22.7 65.7 -6.1 0 -3.1 GTTCGAGCATCTGCTGAAAT

1803 SEQ ID N0:457 -16.6 -22.8 66.2 -4.5 -1.7 -9.7 TGTTCGAGCATCTGCTGAAA

1804 SEQ ID N0:458 -16.6 -22.8 66.1 -4.5 -1.7 -9.7 CCAAGGTGAGGTTTCCTAGA

3114 SEQ ID N0:459 -16.6 -25.2 72.7 -7.9 -0.5 -4.4 ATGGCCAACACCAAGGTGAG

3124 SEQ ID NO:460 -16.6 -25.1 69.3 -6.7 -0.8 -11.7 CAAGGTTGTCTGAGCTAAAT

3429 SEQ ID N0:461 -16.6 -20.6 62.2 -4 0 -5.5 GTCTTGACCTCCAAACTTCT

4898 SEQ ID N0:462 -16.6 -24.5 70.5 -7.4 -0.1 -3.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAAGTGGTTGTAATAGGCTC

6136 SEQ ID N0:463 -16.6 -20.5 62.9 -3.9 0 -3.7 TTTAGTTTGTCAATAATCAT

6301 SEQ ID N0:464 -16.6 -l7 55.2 1.3 0 -2.9 ATTTAGTTTGTCAATAATCA

6302 SEQ ID N0:465 -16.6 -17 55.2 l.3 0 -2.9 CTTTTGATTTTCTCTGACCT

6452 SEQ ID N0:466 -16.6 -22.4 67.4 -5.8 0 -2.4 GGCAGATATGGAAGTTAAAA

6808 SEQ ID N0:467 -16.6 -18 55.5 0 -1.3 -5.5 ATTGAGTAAAATAGAGCAGC

7577 SEQ ID N0:468 -16.6 -18.1 56.7 -1.4 0 -4.1 TTTGTCAGTAGGCAGTATCC

7777 SEQ ID N0:469 -16.6 -24.4 74.3 -7.3 -0.1 -4 AAGAATAGGTTTTGTCAGTA

7787 SEQ TD N0:470 -16.6 -18.8 59.7 -2.2 0 -2.7 TATATTGAGGCAACCATAGT

8127 SEQ ID N0:471 -16.6 -20.9 62.5 -3.7 -0.3 -4 TATCTCATTTATTCTTACAA

348 SEQ ID N0:472 -16.5 -17.4 56.2 -0.7 0 -0.9 ATCATTATCTTGTTCCTTTT

584 SEQ ID N0:473 -16.5 -21.2 65.6 -4.7 0 -1.9 CGACCAGCCTTCACACAGAT

1522 SEQ ID N0:474 -16.5 -26.6 72.6 -10.1 0 -3.2 GCTCCATGGCCATAAAGAGG

2793 SEQ ID N0:475 -16.5 -25.6 70.9 -7.4 0 -11.5 CGATGATGGCCAACACCAAG

3129 SEQ ID N0:476 -16.5 -24.1 65.2 -6.7 -0.8 -6.3 GGTTGTCTGAGCTAAATGAA

3426 SEQ ID N0:477 -16.5 -20.5 62 -4 0 -5.l CATGGATTTCTATAACTTTT

3573 SEQ ID NO:478 -16.5 -18.1 57 -l.6 0 -3.7 TTCTAGCATGGTTTTGATAG

4175 SEQ ID NO:479 -16.5 -20.6 64 -3.4 -0.4 -5 TTTTCTGCTGGTTGAAGTTA

4878 SEQ ID N0:480 -16.5 -21.7 66.5 -5.2 0 -3.7 TTCCTTGGAATTTGTTTGCT

5004 SEQ ID N0:481 -16.5 -22.9 67.7 -5.6 -0.6 -6.3 ATCCTCACTCAGGGGCTCTG

5816 SEQ ID N0:482 -16.5 -28.2 81.2 -10.8 -0.8 -7.6 GGTTGTAATAGGCTCATAAG

6131 SEQ ID N0:483 -16.5 -20.4 62.6 -3.9 0 -3.7 TGGTTGTAATAGGCTCATAA

6132 SEQ ID N0:484 -16.5 -20.4 62.2 -3.9 0 -3.7 TTAGTTTGTCAATAATCATG

6300 SEQ ID N0:485 -16.5 -16.9 54.8 l.3 0 -4.1 CATTTAGTTTGTCAATAATC

6303 SEQ ID N0:486 -16.5 -17 55.2 -0.2 0 -2.9 TCCCATCTGTTTTTTCTGGA

6333 SEQ ID N0:487 -16.5 -25.2 73.7 -8.7 0 -3.5 TCTGTAGTACTGCTTGGTGA

8630 SEQ ID N0:488 -16.5 -24 73 -7 0 -7.8 TGCTTATGCCTCCGCTTCGC

2 SEQ TD N0:489 -16.4 -30.2 80.5 -12.9 -0.8 -5 TTGCCTTTCCTTTATTCATT

747 SEQ ID NO:490 -16.4 -23.5 69.3 -7.1 0 -3 ATCCATTGTGCCATTAAAGT

1352 SEQ ID N0:491 -16.4 -22.5 65.3 -6.l 0 -3.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGACGCTGTCTTCAGATTCG

2049 SEQ ID N0:492 -16.4 -23.9 69.1 -5.8 -1.7 -7.6 GTCCCAGATCAAGAACACAT

2675 SEQ TD N0:493 -16.4 -23 65.9 -6.6 0 -5.4 AGTCCCAGATCAAGAACACA

2676 SEQ ID N0:494 -16.4 -23 66.2 -6.6 0 -5.4 CAGTCCCAGATCAAGAACAC

2677 SEQ ID N0:495 -16.4 -23 66.2 -6.6 0 -5.4 CATCACAGCAGTCCCAGATC

2685 SEQ ID N0:496 -16.4 -26.5 76 -10.1 0 -4.1 CTCCATGGCCATAAAGAGGG

2792 SEQ ID N0:497 -16.4 -25 69.4 -7.4 0 -10.4 CCACCAGATCTTCCCTTTGC

4031 SEQ ID N0:498 -16.4 -28.9 78.9 -12.5 0 -5.6 TTTCTGCTGGTTGAAGTTAT

4877 SEQ ID N0:499 -16.4 -21.6 66.1 -5.2 0 -3.4 CAGGGGCTCTGCACTTTCTT

5807 SEQ ID N0:500 -16.4 -27.5 79.6 -10.5 -0.3 -4.8 CCTGTCTTCCATCTGTATTC

6083 SEQ ID N0:501 -16.4 -25.3 75.2 -8.9 0 -1.6 ACCCTGCTTCACAGAGTTGC

6638 SEQ ID N0:502 -16.4 -27.6 78.4 -9.9 -1.2 -4.8 ACGTTGATACCCTGCTTCAC

6646 SEQ ID N0:503 -16.4 -25.8 72.1 -9.4 0 -4.4 GTTTGGTGTAGTTACAATGT

6757 SEQ ID N0:504 -16.4 -21.1 65.6 -4.2 -0.2 -4.6 TGTTTGGTGTAGTTACAATG

6758 SEQ ID N0:505 -16.4 -19.9 62.1 -3 -0.2 -4.6 TGGCAGATATGGAAGTTAAA

6809 SEQ ID N0:506 -16.4 -18.7 57.3 -0.8 -1.3 -5.5 AAATGATCTAGGTTTGAGTG

8100 SEQ ID N0:507 -16.4 -7.8.6 58.5 -2.2 0 -4.9 CCCAATAAGTGAAATGCAAT

8479 SEQ ID N0:508 -16.4 -19.2 56.2 -2.8 0 -5.6 GAATCATGCACTAGTTTGTT

8612 SEQ ID N0:509 -16.4 -21 64.2 -4.6 0 -5.7 GGTGGTTACTACTATTATTA

8731 SEQ ID N0:510 -16.4 -19.8 62.1 -2.9 -0.1 -3.9 TTCCTTTTTGGGCTTCTTGG

572 SEQ ID N0:511 -16.3 -25.6 74.9 -8.8 -0.2 -3.8 TTTCCCAATAGTCTTGAGTC

1611 SEQ ID N0:512 -16.3 -23.1 69.4 -6.8 0.3 -3.3 CTTCCTGTTGCTTTTTAAGC

1824 SEQ ID N0:513 -16.3 -23.5 69.9 -5.4 -1.8 -6.1 CCTGCTTTCGCCGTCTTCAA

2273 SEQ ID N0:514 -16.3 -28.6 76.9 -11.6 -0.5 -3.6 TGACTAACGTTACTGTTGCG

2332 SEQ ID N0:515 -16.3 -21.7 62.9 -5.4 0.1 -7.8 TTTGCCTTCCAGAGGAATCC

2562 SEQ ID N0:516 -16.3 -26.2 73.6 -8.5 -1.3 -6 AGTGCTCCATGGCCATAAAG

2796 SEQ ID N0:517 -16.3 -25 70.2 -7.4 -0.4 -10.4 GAAGATTCCACCAGATCTTC

4038 SEQ ID N0:518 -16.3 -23 67.5 -4.5 -2.2 -6.8 TCCAGAATGAATATATAGGT

4213 SEQ ID N0:519 -16.3 -18.4 57 -2.1 0 -4.2 ATTCCTTGGAATTTGTTTGC

5005 SEQ ID N0:520 -16.3 -22 65.7 -4.1 -1.5 -8.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGGAAGAGCAGGAGGCCGAT

5443 SEQ ID N0:521 -16.3 -26.4 73 -9.2 -0.8 -7.7 CCAGGAAGAGCAGGAGGCCG

5445 SEQ ID N0:522 -16.3 -28.5 76.2 -11.3 -0.8 -7.7 ACAGGGTTCTTGTATACTGT

6932 SEQ ID N0:523 -16.3 -22.9 70.1 -5.8 -0.6 -6.5 AGGATACATCCCTGTTGGAA

7499 SEQ ID N0:524 -16.3 -24.1 68.9 -6.7 -1 -6.9 GCAGCATAGGCAATATTAAC

7562 SEQ ID N0:525 -16.3 -20.7 61.8 -2.7 -l.6 -6.7 AGTAAAATAGAGCAGCATAG

7573 SEQ TD N0:526 -16.3 -17.8 56 -1.4 0 -5.4 AATGGACAGAGCAGGTTGAA

7650 SEQ ID N0:527 -16.3 -21.5 63.6 -5.2 0 -4.1 ' AAGGTTATATTGAGGCAACC

8132 SEQ ID N0:528 -16.3 -21.1 62.6 -3.7 -1 -5 TGAATCATGCACTAGTTTGT

8613 SEQ ID N0:529 -16.3 -20.9 63.7 -4.6 0 -5.7 GGTGAATCATGCACTAGTTT

8615 SEQ ID N0:530 -16.3 -22.1 66.5 -4.6 -1.1 -6.2 TTTTTTTTTTTTTCCACCTT

9088 SEQ ID N0:531 -16.3 -21.5 65 -5.2 0 0 TTTTTTTTTTTTTTCCACCT

9089 SEQ ID N0:532 -16.3 -21.5 65 -5.2 0 0 CCTGTGCCATCTTTTCATCC

462 SEQ ID N0:533 -16.2 -27.7 78.1 -11.5 0 -2.7 ACGCTGAGACATTGCCCAGG

1095 SEQ ID N0:534 -16.2 -27.6 74.5 -10.9 -0.2 -4.1 ATCTGAGCCATTTCCACAGA

1478 5EQ ID N0:535 -16.2 -25.2 72.2 -7.8 -1.1 -4.9 CACTGGCCTGCATCTGAGCC

1489 SEQ ID N0:536 -16.2 -29.9 81.8 -12.5 -1.1 -7.5 AAAGATTTTCCCAATAGTCT

1617 SEQ ID N0:537 -16.2 -20.1 60.4 -3.9 0 -3 CCAGCAGCACGTAATGTCAA

1642 SEQ ID N0:538 -16.2 -24.5 68.5 -8.3 0 -5.4 AGGCCATGGCCACCACAGCC

1731 SEQ ID N0:539 -16.2 -33 85.5 -12.2 -2.8 -17.4 TGTGCTGTGTTCATCATCAG

2252 SEQ ID N0:540 -16.2 -23.7 72.3 -6.5 -0.9 -4.4 GTCTTCAAATGTGCTGTGTT

2261 SEQ ID N0:541 -16.2 -22.7 68.8 -6.5 , 0 -3.6 CGTTACTGTTGCGTCGCTCT

2325 SEQ ID N0:542 -16.2 -27.2 75.7 -10.4 -0.3 -6.9 ATCGCAGTACAGACAATCCC

3006 SEQ ID N0:543 -16.2 -24.7 69.4 -7.8 -0.4 -5.3 GGAAACACTCCCGCATCTTA

3531 SEQ ID N0:544 -16.2 -25.2 69 -8.4 -0.3 -5.4 TCTGAGGGAAACGAGCTTCA

5195 SEQ ID N0:545 -16.2 -23 66.2 -6.2 -0.3 -5.2 GACGTAGGATTCGGCCAATC

5352 SEQ ID N0:546 -16.2 -25 69.5 -7.6 -0.7 -10 GATCAGACGTAGGATTCGGC

5357 SEQ ID N0:547 -16.2 -24.3 69.7 -7.6 -0.2 -6.6 GGTCGCATCGGGATCAAACT

5867 SEQ ID N0:548 -16.2 -25.1 69.4 -8.9 0 -6.9 GATACCCTGCTTCACAGAGT

6641 SEQ ID N0:549 -16.2 -26 74.4 -8.5 -1.2 -5.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Tnter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGGTGTTTGGTGTAGTTACA

6761 SEQ ID N0:550 -16.2 -23 71.3 -6.8 0 -4.4 TTGTCAGTAGGCAGTATCCA

7776 SEQ ID N0:551 -16.2 -25 75.1 -8.3 -0.2 -4 GAAGAATAGGTTTTGTCAGT

7788 SEQ ID N0:552 -16.2 -19.7 61.7 -3.5 0 -2.7 TTATCTCATTTATTCTTACA

349 SEQ ID N0:553 -16.1 -18.2 58.6 -2.1 0 -0.9 ATTATCTCATTTATTCTTAC

350 SEQ ID N0:554 -16.1 -17.5 57.3 -1.3 0 -0.9 CTTTTTGGGCTTCTTGGCTT

569 SEQ ID N0:555 -16.1 -25.9 76 -8.8 -0.9 -3.7 CTAAGCAAAACCCTCTTGCC

993 SEQ ID N0:556 -16.1 -24.6 67.3 -6.5 -2 -6.2 GTGTTGGTTTCAAAAGCAGA

1324 SEQ ID N0:557 -16.1 -21.2 64 -4.3 -0.6 -1.1 GTGGTGTTGGTTTCAAAAGC

1327 SEQ ID N0:558 -16.1 -22.3 66.9 -5.4 -0.6 -3.8 TGCCATTAAAGTAGGAAGTG

1344 SEQ ID N0:559 -16.1 -20.1 60.4 -4 0 -3.5 TCCATTGTGCCATTAAAGTA

1351 SEQ ID N0:560 -16.1 -22.2 64.8 -6.1 0 -3.1 CCTTCTGGACACTGGCCTGC

1498 SEQ ID N0:561 -16.1 -29.8 81.6 -13.7 0 -7.2 CGCAACTGCCTGAGCTTCTT

1841 SEQ ID N0:562 -16.1 -27.2 74.8 -9.9 -1.1 -5.7 AGGGAGCCACGGATACTCAA

2149 SEQ ID N0:563 -16.1 -25.5 70.7 -8.1 -1.2 -4.5 TGCTTGTTTTGCTATTGCGT

2181 SEQ ID N0:564 -16.1 -24.6 71.7 -6.9 -1.5 -5.8 CTCTTTGCCTTCCAGAGGAA

2565 SEQ ID N0:565 -16.1 -26 73.9 -8.5 -1.3 -5.7 GCAGTCTGAATGATCGCAGT

3018 SEQ ID N0:566 -16.1 -24.4 70.8 -7.6 -0.4 -6.3 GTTCCATTCCCATCTCTAAG

3661 SEQ ID N0:567 -16.1 -25.2 72.6 -9.1 0 -l.6 TTAATACACCCTTCAGTAAA

3976 SEQ ID N0:568 -16.1 -19.5 58.5 -3.4 0 -3.6 GTCATGTATTTGCCCTGGTC

5116 SEQ ID N0:569 -16.1 -26.9 78.3 -10.8 0 -4.7 ATACCCTGCTTCACAGAGTT

6640 SEQ ID N0:570 -16.1 -25.5 73.5 -8.5 -0.7 -5.1 TTTATTCTTACAATATCCCT

341 SEQ ID N0:571 -16 -20.3 61.4 -4.3 0 -2.6 TCCATGGATCACGAAGAAAC

1023 SEQ ID N0:572 -16 -20.3 59 -3.4 0 -9.5 GGCCATGGCCACCACAGCCA

1730 SEQ ID N0:573 -16 -33.7 86 -13.4 -3.l -16.7 GCTCTCTCTGTCTTCTTTTC

1983 SEQ ID N0:574 -16 -25.2 79.4 -9.2 0 -2.8 CCATGGCCATAAAGAGGGTA

2790 SEQ ID N0:575 -16 -24.6 68.6 -7.4 0 -10.4 TAGTGCTCCATGGCCATAAA

2797 SEQ ID N0:576 -l6 -24.7 69.4 -7.4 -0.4 -10.4 ACAATCAGGAAGGAGTGGAA

3259 SEQ ID N0:577 -16 -20.5 61.2 -3.7 -0.6 -4.5 TCCCATCTCTAAGATAATTA

3654 SEQ ID N0:578 -16 -20.1 60.7 -3.4 -0.5 -4.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCCATTCCCATCTCTAAGAT

3659 SEQ ID N0:579 -l6 -24.5 70.2 -7.9 -0.3 -3.5 TGTTACCCGTTGTCATGTTA

4566 SEQ ID N0:580 -16 -24.6 71.1 -8.6 0 -4.7 ACTTCTTTTTCTGCTGGTTG

4884 SEQ ID N0:581 -16 -23.4 71.3 -7.4 0 -3.7 ATAAACCTGTCTTCCATCTG

6088 SEQ ID N0:582 -16 -22.4 65.6 -6.4 0 -2 TGGCATGGACCTCCTCTTGA

6549 SEQ ID N0:583 -16 -27.9 77.8 -11.4 -0.1 -5.l GTCTAGGTAGCCATTGGGTT

6713 SEQ ID N0:584 -16 -26.6 78.7 -10 -0.3 -6.3 ATGGCAGATATGGAAGTTAA

6810 SEQ ID N0:585 -16 -19.4 59.2 -2 -1.3 -5.5 ATCATGCACTAGTTTGTTGT

8610 SEQ ID N0:586 -16 -22.3 68.5 -6.3 0 -5.7 ~

TTAAGCTGTTCGAGCATCTG

1810 SEQ ID N0:587 -15.9 -22.7 67.1 -4.5 -2.3 -7.3 GTGCTGTGTTCATCATCAGC

2251 SEQ ID N0:588 -15.9 -25.5 77.3 -6.5 -3.1 -7.7 AAGGTTGTCTGAGCTAAATG

3428 SEQ ID N0:589 -15.9 -19.9 60.9 -4 0 -5.5 .

TATTTCAATTCCAGTATTAT

3623 SEQ ID N0:590 -15.9 -18.2 57.6 -2.3 0 -2.5 TTGCTTATTTCAATTCCAGT

3628 SEQ ID N0:591 -15.9 -21.6 65.2 -5.7 0 -3.6 CTTGTTCACCTTCTCGGGGT

3915 SEQ ID N0:592 -15.9 -27.9 79.4 -11.1 -0.7 -4 CAGACGTAGGATTCGGCCAA

5354 SEQ ID N0:593 -15.9 -25.3 69.4 -8.8 -0.3 -7.2 TCATCCTCACTCAGGGGCTC

5818 SEQ ID N0:594 -15.9 -28.4 82.4 -11.6 -0.8 -7.6 GTTGTAATAGGCTCATAAGA

6130 SEQ ID N0:595 -15.9 -19.8 61.3 -3.9 0 -3.8 AGTCTAGGTAGCCATTGGGT

6714 SEQ ID N0:596 -15.9 -26.5 78.7 -10 -0.3 -6.7 GGGTTCTTGTATACTGTTTC

6929 SEQ TD N0:597 -15.9 -22.6 70.5 -6.7 0 -6.3 TAGGTTTGAGTGTTTGACCA

8092 SEQ TD N0:598 -15.9 -23 69.2 -5.8 -1.2 -5.9 TTTTTTTTTTTTCCACCTTA

9087 SEQ ID N0:599 -15.9 -21.1 64 -5.2 0 -0.2 GCAGCACGTTTTTCGATAGC

541 SEQ ID N0:600 -15.8 -24.9 71.1 -8.1 -0.9 -5.8 TGAATCCATTGTGCCATTAA

1355 SEQ ID N0:601 -15.8 -21.9 63.3 -6.1 0 -3.1 AATGTGCTGTGTTCATCATC

2254 SEQ ID N0:602 -15.8 -22.3 68.1 -6.5 0 -3.l CTGTTGCGTCGCTCTCCATG

2320 SEQ ID N0:603 -15.8 -28.3 78.2 -12.5 0.4 -6.2 CATGGGGTAGTGCTCCATGG

2804 SEQ ID N0:604 -15.8 -27.4 77.8 -7.9 -3.7 -12.3 AATACACCCTTCAGTAAAAC

3974 SEQ ID N0:605 -15.8 -19.2 57.4 -3.4 0 -3.6 CCAATGCACCACTACTGAGA

4122 SEQ ID N0:606 -15.8 -24.5 68.2 -8.7 0 -5.9 AGTTTACTTTCACGTTTTTC

4650 SEQ ID N0:607 -15.8 -20.5 64 -4.7 0 -4.7 kcal) mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GCCAGCGCCAACATTATCAA

4670 SEQ ID N0:608 -15.8 -26 70 -9.7 0 -7.6 TTCTGAGGTTTCTTGGATCC

4969 SEQ ID N0:609 -15.8 -24.2 72.8 -7.5 0 -9.6 TAAACATGACCAGGAAGAGC

5454 SEQ ID N0:610 -15.8 -20.2 59.6 -4.4 0 -5.2 CGGTCTCCCTTAACTGAGCT

5674 SEQ ID N0:611 -15.8 -27.3 75.6 -10.9 -0.3 -6.2 GGATCCAGGGCAGCTGCAAA

5917 SEQ ID N0:612 -15.8 -27.6 75.7 -8.9 -0.7 -14 CTTCCATCTGTATTCGAAGG

6078 SEQ ID N0:613 -15.8 -22.7 66.4 -6.9 0 -7.1 ACCTCCTCTTGAAGTCCAGT

6541 SEQ ID N0:614 -15.8 -27.2 77.7 -11.4 0 -3 GGCATGGACCTCCTCTTGAA

6548 SEQ ID N0:615 -15.8 -27.2 75.5 -11.4 0 -5 GACAGGGTTCTTGTATACTG

6933 SEQ ID N0:616 -15.8 -22.3 68 -5.8 -0.4 -6.5 TATTTTTCTAGAATGGATTT

7255 SEQ ID N0:617 -15.8 -17 55.1 -1.1 0 -7.4 TTAATAGAAGTTGTTTATCA

7441 SEQ ID N0:618 -15.8 -16.2 53.4 0.4 0 -2.9 ATCCCTGTTGGAAGCTTGCA

7492 SEQ ID N0:619 -15.8 -27 75.6 -10.1 -1 -7.6 CATCCCTGTTGGAAGCTTGC

7493 SEQ ID N0:620 -15.8 -27 75.6 -10.1 -1 -7.2 ACATGCATTAGTGATAGCCT

7544 SEQ ID N0:621 -15.8 -23.4 68.7 -7.6 0 -6.8 GGTAGAAAATGATCTAGGTT

8106 SEQ ID N0:622 -15.8 -18.7 58.3 -2.2 -0.4 -4.9 ACTTTGCCACTTTGTTCATG

8277 SEQ ID N0:623 -15.8 -23.5 69.2 -7.7 0 -4.1 TTTTTTTTTTTCCACCTTAT

9086 SEQ ID N0:624 -15.8 -21 63.7 -5.2 0 -0.5 CATGGATCACGAAGAAACGT

1021 SEQ ID N0:625 -15.7 -19.9 57.7 -3.4 -0.6 -6 CCATTGTGCCATTAAAGTAG

1350 SEQ ID N0:626 -15.7 -21.8 63.6 -6.l 0 -3.1 TGTCCCATTTGAATCCATTG

1364 SEQ ID N0:627 -15.7 -23.6 67.2 -7.9 0 -2.3 AGCAGCACGTAATGTCAACT

1640 SEQ ID N0:628 -15.7 -22.9 66.3 -7.2 0 -5.4 CTTCAAATGTGCTGTGTTCA

2259 SEQ ID N0:629 -15.7 -22.2 66.6 -6.5 0 -3.6 CCTCTGGGGGAAGTTGTCCA

2472 SEQ ID NO:630 -15.7 -28.5 79.7 -10.8 -2 -6.3 GCCCTCTGGGGGAAGTTGTC

2474 SEQ ID N0:631 -15.7 -29.6 83.2 -11.7 -2.2 -8.7 AGTAGCAGCAAGGTTGTCTG

3437 SEQ ID N0:632 -15.7 -24.2 73.1 -8 -0.2 -7 CTTTGCTTATTTCAATTCCA

3630 SEQ ID N0:633 -15.7 -21.4 64.1 -5.7 0 -3.6 TCACCTTCTCGGGGTAGAAC

3910 SEQ ID N0:634 -15.7 -25.4 72.5 -8.7 -0.9 -4.9 GCACCACTACTGAGAAGGAT

4117 SEQ ID N0:635 -15.7 -23.6 67.7 -7 -0.8 -4.7 TGCACCACTACTGAGAAGGA

4118 SEQ ID N0:636 -15.7 -23.6 67.6 -7 -0.8 -6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CTGAGGGAAACGAGCTTCAG

5194 SEQ ID N0:637 -15.7 -22.6 65 -6.2 -0.5 -5.2 ATCAGACGTAGGATTCGGCC

5356 SEQ ID N0:638 -15.7 -25.7 72 -9.5 -0.2 -5.8 ACCAGGAAGAGCAGGAGGCC

5446 SEQ ID N0:639 -15.7 -27.9 77.1 -11.3 -0.8 -6.4 ACTGGGTCGCATCGGGATCA

5871 SEQ ID N0:640 -15.7 -27.7 76.3 -12 0 -6.9 CCTCCTCTTGAAGTCCAGTT

6540 SEQ ID N0:641 -15.7 -27.1 77.4 -11.4 0 -3 CTTCACTGTCTTGTATAGGC

6602 SEQ ID N0:642 -15.7 -23.4 71.6 -7.7 0 -2.8 TACCCTGCTTCACAGAGTTG

6639 SEQ ID N0:643 -15.7 -25.5 73.3 -8.5 -1.2 -5.1 CCCTATAGTCTAGGTAGCCA

6720 SEQ ID N0:644 -15.7 -27.1 77.6 -10 -1.3 -5.6 GGTGTAGTTACAATGTTCAC

6753 SEQ ID N0:645 -15.7 -21.1 65.4 -4.9 -0.2 -4.6 AAAGGTGTTTGGTGTAGTTA

6763 SEQ ID N0:646 -15.7 -20.7 64.4 -5 ~ 0 -1.9 TAAAGGTGTTTGGTGTAGTT

6764 SEQ ID N0:647 -15.7 -20.7 64.4 -5 0 -2.2 TATGGCAGATATGGAAGTTA

6811 SEQ ID N0:648 -15.7 -19.8 60.7 -3.3 -0.6 -5.5 GGGTATTTAATTGACATAGA

7360 SEQ ID N0:649 -15.7 -18.4 57.7 -2.7 0 -2.9 TCAGGCTATATATATATTTG

7424 SEQ ID N0:650 -15.7 -17.7 56.7 -2 0 -6.7 ATCAGGCTATATATATATTT

7425 SEQ ID N0:651 -15.7 -17.7 56.8 -2 0 -6.7 ACAGGATACATCCCTGTTGG

7501 SEQ ID N0:652 -15.7 -25.1 71.5 -6.7 -2.7 -8 CATGCATTAGTGATAGCCTT

7543 SEQ ID N0:653 -15.7 -23.3 68.5 -7.6 0 -6.8 AAAATGATCTAGGTTTGAGT

8101 SEQ ID N0:654 -15.7 -17.9 56.6 -2.2 0 -4.2 TGGTAGAAAATGATCTAGGT

8107 SEQ ID N0:655 -15.7 -18.6 57.9 -2.2 -0.4 -4.9 AGGCAACCATAGTTGGTAGA

8120 SEQ ID N0:656 -15.7 -23.6 69.1 -5.5 -2.4 -8.7 GTGAATCATGCACTAGTTTG

8614 SEQ ID N0:657 -15.7 -20.9 63.7 -4.6 , -0.3 -5.7 GTGGTTACTACTATTATTAT

8730 SEQ ID N0:658 -15.7 -18.6 59.4 -2.9 0 -3 CTCATTTATTCTTACAATAT

345 SEQ ID N0:659 -15.6 -17 54.9 -1.3 0 -2.4 GTGCCATTAAAGTAGGAAGT

1345 SEQ ID N0:660 -15.6 -21.3 63.5 -5.7 0 -3.5 GTATGTTTTCCCAGCAGCAC

1652 SEQ ID N0:661 -15.6 -26.7 77 -11.1 0 -5.4 TGCTCCATGGCCATAAAGAG

2794 SEQ ID N0:662 -15.6 -24.4 68.4 -7.4 -0.4 -10.8 TCGCAGTACAGACAATCCCT

3005 SEQ ID N0:663 -15.6 -25.6 71.3 -10 0.4 -4.5 GACGATGATGGCCAACACCA

3131 SEQ ID N0:664 -15.6 -25.6 68.6 -9.2 -0.6 -5.8 ACCACAAGGTTTCCAATGAC

3376 SEQ ID N0:665 -15.6 -22.9 65.5 -6.2 -1 -4.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCATGGATTTCTATAACTTT

3574 SEQ ID N0:666 -15.6 -18.4 58.1 -2.8 0 -5 TTTTAATACACCCTTCAGTA

3978 SEQ ID N0:667 -15.6 -21.1 63.1 -5.5 0 -3.4 CTGCTGGTTGAAGTTATCTA

4874 SEQ ID N0:668 -15.6 -22 66.8 -6.4 0 -3.6 AAGCAAAGAGCAGCGTGCGG

5394 SEQ ID N0:669 -15.6 -25.1 68.5 -7.3 -2.2 -8.5 ATCTGTATTCGAAGGGCATC

6073 SEQ ID N0:670 -15.6 -22.7 67.2 -7.1 0 -7.l TTGGTGTAGTTACAATGTTC

6755 SEQ ID N0:671 -15.6 -20.3 63.8 -4.2 -0.2 -4.6 GGTTCTTGTATACTGTTTCT

6928 SEQ ID N0:672 -15.6 -22.3 69.8 -6.7 0 -6.3 AGGGTATTTAATTGACATAG

7361 SEQ ID N0:673 -15.6 -17.8 56.5 -2.2 0 -2.9 TCTAGGTTTGAGTGTTTGAC

8094 SEQ ID N0:674 -15.6 -21.6 67.7 -6 0 -4 TATTGAGGCAACCATAGTTG

8125 SEQ ID N0:675 -15.6 -21.3 63.3 -3.7 -2 -5.6 CCAATAAGTGAAATGCAATA

8478 SEQ ID N0:676 -15.6 -16.9 52.3 -1.2 0 -5.6 ATGCACTAGTTTGTTGTAAA

8607 SEQ ID N0:677 -15.6 -19.5 60.5 -3.9 0 -5.7 CTGCCTGAGCTTCTTCCTGT

1836 SEQ ID N0:678 -15.5 -28.9 82.3 -12.2 -1.1 -5.2 TACTCCACTGAAATCTCTTG

1877 SEQ ID N0:679 -15.5 -20.9 62.6 -5.4 0 -2.5 GACGCTGTCTTCAGATTCGG

2048 SEQ ID N0:680 -15.5 -25.1 71.8 -8.1 -1.4 -6.8 TTGCTATTGCGTCTTGGGGA

2173 SEQ TD N0:681 -15.5 -26 74.3 -8.9 -1.5 -6.6 CTTTGCCCGACCTCTGAAAC

2207 SEQ ID N0:682 -15.5 -26.1 70.1 -10.6 0 -3 CTTTCGCCGTCTTCAAATGT

2269 SEQ ID N0:683 -15.5 -24.4 68.5 -8.9 0 -2.8 CACAATCAGGAAGGAGTGGA

3260 SEQ ID N0:684 -15.5 -21.9 64.5 -5.6 -0.6 -4.5 ATGGTTTGGCCAGCGACCTC

3328 SEQ ID N0:685 -15.5 -29 79.2 -11.7 -1.4 -11.4 TGTCTGAGCTAAATGAACTC

3423 SEQ ID N0:686 -15.5 -19.5 59.9 -4 0 -5.1 GTTGTCTGAGCTAAATGAAC

3425 SEQ TD N0:687 -15.5 -19.5 60 -4 0 -5.1 GCTTATTTCAATTCCAGTAT

3626 SEQ ID N0:688 -15.5 -21.2 64.4 -5.7 0 -2.8 TGCTTATTTCAATTCCAGTA

3627 SEQ ID N0:689 -15.5 -21.2 64.3 -5.7 0 -3.6 TTGTTCACCTTCTCGGGGTA

3914 SEQ ID N0:690 -15.5 -26.7 76.8 -10.2 -0.9 -4.2 AGCTTGTTCACCTTCTCGGG

3917 SEQ ID NO:691 -15.5 -27.3 78 -11.3 -0.2 -4.5 CAGCTTGTTCACCTTCTCGG

3918 SEQ ID N0:692 -15.5 -26.8 76.4 -11.3 0 -4.5 TCTTGACCTCCAAACTTCTT

4897 SEQ ID N0:693 -15.5 -23.4 67.6 -7.4 -O.l -2.7 CAGCGTGCGGATCCCCTTTG

5384 SEQ ID N0:694 -15.5 -30.4 79.1 -14.2 -0.4 -7.5 kcal/

mol kcal/moldeg kcal/molkcal/mo1kcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATCTGTTTTTTCTGGAGTGG

6329 SEQ ID N0:695 -15.5 -22.5 69.5 -7 0 -2.7 CTTTTTCTGGTTTGTCTTTC

6417 SEQ TD N0:696 -15.5 -22.3 70.2 -6.8 0 -l.5 TTCTTTTTCTGGTTTGTCTT

6419 SEQ ID N0:697 -15.5 -22.3 70.2 -6.8 0 -1.5 TTGGCATGGACCTCCTCTTG

6550 SEQ ID N0:698 -15.5 -27.4 76.8 -11.4 -0.1 -5.1 AGGGTTCTTGTATACTGTTT

6930 SEQ ID N0:699 -15.5 -22.2 69 -6.7 0 -6.3 TCCCTGTTGGAAGCTTGCAA

7491 SEQ ID N0:700 -15.5 -26.3 73.2 -10.1 -0.5 -7.6 GGACAGAGCAGGTTGAATTC

7647 SEQ ID N0:701 -15.5 -22.7 67.8 -7.2 0 -6.4 TTATATTGAGGCAACCATAG

8128 SEQ TD N0:702 -15.5 -19.8 59.8 -3.7 -0.3 -4 TTTTTTTTTTTTTTTCCACC

9090 SEQ ID N0;703 -15.5 -20.7 63.3 -5.2 0 0 TTTTTTTTTTTTTGACCACA

103 SEQ ID N0;704 -15,4 -19.6 60.4 -4.2 0 -2.1 ATGTCCCATTTGAATCCATT

1365 SEQ ID N0:705 -15,4 -23.6 67,3 -8.2 0 -2.4 CAAATGTGCTGTGTTCATCA

2256 SEQ ID N0:706 -15.4 -21.9 65.5 -6.5 0 -3.6 TCTTCAAATGTGCTGTGTTC

2260 SEQ ID N0:707 -15,4 -21.9 67 -6.5 0 -3.6 ACGTTACTGTTGCGTCGCTC

2326 SEQ ID N0:708 -15.4 -26.5 74,3 -10.4 -0.4 -7.1 CTTTGCCTTCCAGAGGAATC

2563 SEQ ID N0:709 -15.4 -25.1 71.9 -8.5 -1.1 -6 GACCAAGCTCCATTAAACTG

2973 SEQ ID N0:710 -15.4 -22.1 63.1 -6.7 0 -4.5 GGAAGGAGTGGAAGAAGTCG

3252 SEQ TD N0:711 -15.4 -21.5 63.2 -5.6 -0.2 -2.7 CACATGGTTTGGCCAGCGAC

3331 SEQ TD N0:712 -15.4 -27.3 74.8 -10.4 -1.4 -8.8 TATTGCCTTCATGGATTTCT

3582 SEQ ID N0:713 -15.4 -23 68.2 -7.6 0.4 -4.7 TCTTTGCTTATTTCAATTCC

3631 SEQ ID N0:714 -15.4 -21.1 64.4 -5.7 0 -2.9 CCTTTGCCTTCTTCTGTACT

4018 SEQ ID N0:715 -15.4 -26.6 77 -11.2 0 -4.8 GCCAACATTATCAAAGTTTA ' 4664 SEQ ID N0:716 -15.4 -19.3 58.4 -3.9 0 -4.1 GATGTCTTGACCTCCAAACT

4901 SEQ ID N0:717 -15.4 -23.7 67.8 -7.4 -0.7 -4.9 TGACCAGGAAGAGCAGGAGG

5448 SEQ ID N0:718 -15.4 -24.7 70.4 -9.3 0 -4.1 GGCATAGATAAACATGACCA

5462 SEQ ID N0:719 -15.4 -20.7 60.5 -4.4 -0.8 -5.2 CCCAGCCAGCAGAGGTTGTA

5586 SEQ ID N0:720 -15.4 -29.7 82.1 -13.4 -0.8 -5.2 GAGGAGGATCCAGGGCAGCT

5922 SEQ ID N0:721 -15.4 -28.9 81.6 -10.9 -2.6 -9.3 TTTTCTGGTTTGTCTTTCTC

6415 SEQ ID N0:722 -15.4 -22.6 71.6 -7.2 0 -1.5 TCCCTATAGTCTAGGTAGCC

6721 SEQ ID N0:723 -15.4 -26.8 78.3 -10 -1.3 -5.6 kcal/

kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTTATCAGGCTATATATATA

7428 SEQ ID N0:724 -15.4 -17.4 56.2 -2 0 -6 AGGTTTGAGTGTTTGACCAA

8091 SEQ ID N0:725 -15.4 -22.6 67.4 -5.9 -1.2 -5.9 TGAGGCAACCATAGTTGGTA

8122 SEQ ID N0:726 -15.4 -23.6 68.7 -5.8 -2.4 -8.7 CTTTGTTCTGTAGTACTGCT

8636 SEQ ID N0:727 -15.4 -23.3 71.8 -7.4 0 -7.8 TTTTTTTTTTCCACCTTATA

9085 SEQ ID N0:728 -15.4 -20.6 62.7 -5.2 0 -1.7 TCTCATTTATTCTTACAATA

346 SEQ ID N0:729 -15.3 -17.4 56.2 -2.1 0 -2.2 AAATTGCCTTTCCTTTATTC

750 SEQ ID N0:730 -15.3 -21.3 63.3 -6 0 -3.2 TTCCCAATAGTCTTGAGTCA

1610 SEQ ID N0:731 -15.3 -23.7 70.2 -7.9 -0.2 -5.5 GGTTCCTCCATTCTTTAGCA

1956 SEQ ID N0:732 -15.3 -26.7 77.7 -10.8 -0.3 -4.7 GCTGTCTTCAGATTCGGATT

2045 SEQ ID N0:733 -15.3 -24.2 71.6 -7.4 -1.4 -5.7 CTGCTTTCGCCGTCTTCAAA

2272 SEQ ID N0:734 -15.3 -25.9 71.3 -9.9 -0.5 -3.6 AACCTTCTCTTTCTGACTTC

2509 SEQ ID N0:735 -15.3 -22.8 68.9 -7.5 0 -2.6 ACATTGGCAAATCTATACCA

2659 SEQ ID N0:736 -15.3 -20.9 61.3 -5.1 -0.2 -4.4 ' TCCCAGATCAAGAACACATT

2674 SEQ ID N0:737 -15.3 -21.9 63.3 -6.6 0 -4.6 ATGGGGTAGTGCTCCATGGC

2803 SEQ ID N0:738 -15.3 -28.5 81.3 -11.2 -2 -8.9 CTCAGTCATGGGGTAGTGCT

2810 SEQ ID N0:739 -15.3 -26.7 79.7 -11.4 0 -4.7 GCGGAACACAATCAGGAAGG

3266 SEQ ID N0:740 -15.3 -22.2 62.9 -6.9 0 -3.4 TAATACACCCTTCAGTAAAA

3975 SEQ ID N0:741 -15.3 -18.7 56.4 -3.4 0 -3.6 TTTTTAATACACCCTTCAGT

3979 SEQ ID N0:742 -15.3 -21.5 64 -6.2 0 -2.6 TCCAAACTTCTTTTTCTGCT

4889 SEQ ID N0:743 -15.3 -22.6 66.9 -7.3 0 -3.6 TGTCTTGACCTCCAAACTTC

4899 SEQ ID N0:744 -15.3 -23.6 68.4 -7.4 , -0.7 -4.9 GGGTTTCTGAGGTTTCTTGG

4973 SEQ ID N0:745 -15.3 -24.9 75.4 -9.6 0 -2.5 GGAAGAGCAGGAGGCCGATG

5442 SEQ ID N0:746 -15.3 -26.4 72.6 -10.2 -0.8 -7.7 ATTTTTCTAGAATGGATTTA

7254 SEQ ID N0:747 -15.3 -17 55.1 -1.1 0 -7.7 TAGGGTATTTAATTGACATA

7362 SEQ ID N0:748 -15.3 -17.5 55.8 -2.2 0 -2.9 TATCAGGCTATATATATATT

7426 SEQ ID N0:749 -15.3 -17.3 55.9 -2 0 -6.7 TTATCAGGCTATATATATAT

7427 SEQ ID N0:750 -15.3 -17.3 55.9 -2 0 -6.5 GTTGGTAGAAAATGATCTAG

8109 SEQ ID N0:751 -15.3 -17.5 55.7 -2.2 0.1 -4.9 TAGTTGGTAGAAAATGATCT

8111 SEQ ID N0:752 -15.3 -17.5 55.7 -2.2 0 -4.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGCCCAATAAGTGAAATGCA

8481 SEQ ID N0:753 -15.3 -21.7 61.7 -6.4 0 -5.2 GTAGTACTGCTTGGTGAATC

8627 5EQ ID N0:754 -15.3 -22.4 68.4 -7.1 0 -7 TTTTTTTTTCCACCTTATAT

9084 SEQ ID N0:755 -15.3 -20.5 62.4 -5.2 0 -2 TCCAGCCAGTTCCATGGATC

1033 SEQ ID N0:756 -15.2 -28.2 79.3 -11.7 -l -10 CCTGCATCTGAGCCATTTCC

1483 SEQ ID N0:757 -15.2 -28.4 78.5 -12.7 -0.1 -4.9 CCAATAGTCTTGAGTCATGA

1607 SEQ ID N0:758 -15.2 -21.8 65.6 -6.1 -0.2 -7.3 GGAGCCACGGATACTCAAGA

2147 SEQ ID N0:759 -15.2 -24.9 69.5 -8.4 -1.2 -4.5 TCTTTGCCTTCCAGAGGAAT

2564 SEQ ID N0:760 -15.2 -25.1 71.9 -8.5 -1.3 -6 ACGGCTCTTTGCCTTCCAGA

2569 SEQ ID N0:761 -15.2 -28.9 79.4 -11 -2.7 -6.4 CCAACACCAAGGTGAGGTTT

3120 SEQ ID N0:762 -15.2 -24.7 69.3 -8.4 -0.8 -9.7 TTGTCTGAGCTAAATGAACT

3424 SEQ ID N0:763 -15.2 -19.2 58.9 -4 0 -5.1 TGGTTCCATTCCCATCTCTA

3663 SEQ ID N0:764 -15.2 -27.1 77.3 -11.9 0 -4.8 TTCCAGAATGAATATATAGG

4214 SEQ ID N0:765 -15.2 -17.3 54.4 -2.l 0 -4.2 AAACATGTTACCCGTTGTCA

4571 SEQ ID N0:766 -15.2 -23.1 65.6 -7.2 0 -9 GTGCAAGATAGCCAGCGCCA

4680 SEQ ID N0:767 -15.2 -28.9 77.5 -12.9 -0.6 -7.7 AGGCTGAAGTTTAACATCTC

4760 SEQ ID N0:768 -15.2 -20.8 63.7 -5.6 0 -4.6 CTTGACCTCCAAACTTCTTT

4896 SEQ ID N0:769 -15.2 -23.1 66.5 -7.4 -0.1 -2.7 TGAGGGAAACGAGCTTCAGC

5193 SEQ ID N0:770 -15.2 -23.5 67.2 -8.3 0.4 -5.3 GTCTGAGGGAAACGAGCTTC

5196 SEQ ID N0:771 -15.2 -23.5 68.3 -8.3 0 -5.2 GTGCGGATCCCCTTTGCTCC

5380 SEQ ID N0:772 -15.2 -32.2 85.1 -16.3 -0.4 -7.5 AGCAAAGAGCAGCGTGCGGA

5393 SEQ ID N0:773 -15.2 -26.4 71.8 -9 , -2.2 -8.5 GACCAGGAAGAGCAGGAGGC

5447 SEQ ID N0:774 -15.2 -26.5 74.9 -10.6 -0.4 -4.8 TTCAAAGTGGTTGTAATAGG

6139 SEQ ID N0:775 -15.2 -18.6 58.3 -2.7 -0.4 -3.8 GACCTCCTCTTGAAGTCCAG

6542 SEQ ID N0:776 -15.2 -26.6 75.5 -11.4 0 -3.3 CTATAGTCTAGGTAGCCATT

6718 SEQ ID N0:777 -15.2 -23.2 70.3 -7.4 -0.3 -4.6 ATATTTTTCTAGAATGGATT

7256 SEQ ID N0:778 -15.2 -16.9 54.7 -1.1 0 -7.7 TCAGCTTTGCTGAGAGCAGA

7337 SEQ ID N0:779 -15.2 -25 74 -6.7 -3.1 -11.4 TTTAATAGAAGTTGTTTATC

7442 SEQ ID N0:780 -15.2 -15.6 52.4 0.4 0 -2.5 GGTTTGAGTGTTTGACCAAT

8090 SEQ ID N0:781 -15.2 -22.6 67.1 -6.7 -0.4 -5.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GAGCCCAATAAGTGAAATGC

8482 SEQ ID N0:782 -15.2 -21.6 61.8 -6.4 0 -3.3 CAGGTATCTCATCCCTGTCA

268 SEQ ID N0:783 -15.1 -26.8 77.9 -10.4 -1.2 -4.9 ATTTATTCTTACAATATCCC

342 SEQ ID N0:784 -15.1 -19.4 59.4 -4.3 0 -2.6 GAATCCATTGTGCCATTAAA

1354 SEQ ID N0:785 -15.1 -21.2 61.4 -6.1 0 -3.1 GACCAGCCTTCACACAGATG

1521 SEQ ID N0:786 -15.1 -25.8 72.5 -10.7 0 -2.6 TCAAATGTGCTGTGTTCATC

2257 SEQ ID N0:787 -15.1 -21.6 65.8 -6.5 0 -3.6 CGTCTTCAAATGTGCTGTGT

2262 SEQ ID N0:788 -15.1 -23.4 68.6 -8.3 0 -3.6 CCAAGCTCCATTAAACTGAG

2971 SEQ ID N0:789 -15.1 -21.9 62.8 -6.8 0 -5 TAACTTTTGGCTTTCTAAAA

3561 SEQ ID N0:790 -15.1 -17.4 54.8 -1.6 -0.4 -4.3 ATTTCAATTCCAGTATTATT

3622 SEQ ID N0:791 -15.1 -18.6 58.5 -3.5 0 -2.3 TTATTTCAATTCCAGTATTA

3624 SEQ ID N0:792 -15.1 -18.3 57.9 -3.2 0 -2.5 ATACACCCTTCAGTAAAACA

3973 SEQ ID N0:793 -15.1 -20.6 60.4 -5.5 0 -3.6 ATGCACCACTACTGAGAAGG

4119 SEQ ID N0:794 -15.1 -23 66.3 -7 -0.8 -6.8 ATGGTTTTGATAGTCTTTCG

4168 SEQ ID N0:795 -15.1 -20.9 64.3 -5.8 0 -2.9 CTGAGGTTTCTTGGATCCAA

4967 SEQ ID N0:796 -15.1 -23.7 69.4 -6.9 -0.2 -11.5 GCATAGATAAACATGACCAG

5461 SEQ ID N0:797 -15.1 -19.5 58.3 -4.4 0 -5.2 ACGGTCTCCCTTAACTGAGC

5675 SEQ ID N0:798 -15.1 -26.6 74.3 -10.9 -0.3 -3.7 GTCGCATCGGGATCAAACTT

5866 SEQ ID N0:799 -15.1 -24 67.3 -8.9 0 -6.9 AATCCTCCCTTTAATTGCCT

6260 SEQ ID N0:800 -15.1 -25.9 71.2 -10.8 0 -3 CCATTTAGTTTGTCAATAAT

6304 SEQ ID N0:801 -15.1 -18.6 57.8 -3.5 0 -2.9 TTTCTGGTTTGTCTTTCTCA

6414 SEQ ID N0:802 -15.1 -23.2 72.5 -8.1 0 -1.7 TTGATACCCTGCTTCACAGA

6643 SEQ ID N0:803 -15.1 -24.9 71 -8.5 -1.2 -3.8 CAGCTTTGCTGAGAGCAGAA

7336 SEQ ID N0:804 -15.1 -23.9 69.8 -6.7 -2.1 -10.5 GAAAATGATCTAGGTTTGAG

8102 5EQ ID N0:805 -15.1 -17.3 54.9 -2.2 0 -4.9 AGAAAATGATCTAGGTTTGA

8103 SEQ ID N0:806 -15.1 -17.3 54.9 -2.2 0 -4.9 GTACTGCTTGGTGAATCATG

8624 SEQ ID N0:807 -15.1 -22.2 66.4 -7.1 0 -4.6 AGTACTGCTTGGTGAATCAT

8625 SEQ ID N0:808= -15.1 -22.2 66.8 -7.1 0 -5.5 GTTCAGCTTTTTTTTTTTTT

118 SEQ ID N0:809 -15 -20.3 64.5 -5.3 0 -4.5 CGTGTAGCTTCTTGCATACG

428 SEQ ID N0:810 -15 -24.3 69.7 -7.5 -1.8 -6.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TATAAGTGACTCAAAGGTAT

965 SEQ ID N0:811 -15 -17.4 55.3 -2.4 0 -3.3 GCCTGCATCTGAGCCATTTC

1484 SEQ ID N0:812 -15 -28.2 79.4 -12.7 -0.1 -4.9 TAAAGATTTTCCCAATAGTC

1618 SEQ ID N0:813 -15 -18.9 58 -3.9 0 -3.2 CCACCTACTCCACTGAAATC

1882 SEQ ID N0:814 -15 -24.4 67.9 -9.4 0 -1.7 GTCTGAGCTAAATGAACTCA

3422 SEQ ID N0:815 -15 -20.2 61.2 -4 -1.1 -4.6 GCTCTTTGCTTATTTCAATT

3633 SEQ ID N0:816 -15 -21.4 65.3 -5.7 -0.4 -3.6 TTCGGGTTCAGTTTCAGCTT

3932 SEQ ID N0:817 -15 -25.4 75.6 -10.4 0 -4.5 AAGTTTACTTTCACGTTTTT

4651 SEQ ID N0:818 -15 -19.4 60.3 -3.9 -0.1 -4.9 TGCAAGATAGCCAGCGCCAA

4679 SEQ ID N0:819 -15 -27 72.1 -11.2 -0.6 -7.7 GCGGATCCCCTTTGCTCCTT

5378 SEQ TD N0:820 -15 -32 84.1 -16.3 -0.4 -7.5 GTTTCAAAGTGGTTGTAATA

6141 SEQ ID N0:821 -15 -18.7 58.9 -3 -0.4 -3.8 TTTCTTTTTCTGGTTTGTCT

6420 SEQ ID N0:822 -15 -22.3 70.2 -7.3 0 -1.5 TGATACCCTGCTTCACAGAG

6642 SEQ ID N0:823 -15 -24.8 70.9 -8.5 -1.2 -3.7 AACGTTGATACCCTGCTTCA

6647 SEQ ID N0:824 -15 -24.9 69.3 -9.9 0 -6.4 GGCAACCATAGTTGGTAGAA

8119 SEQ ID N0:825 -15 -22.9 66.6 -5.5 -2.4 -8.7 GCTTGGTGAATCATGCACTA

8619 SEQ ID N0:826 -15 -23.5 68.8 -7.2 -1.2 -5.5 AAAGCTGCTTTTAATTTGCC

8655 SEQ ID N0:827 -15 -21.3 62.7 -5 -0.1 -10.6 ATATTGAAAGAAGATGAGTT

8798 SEQ ID N0:828 -15 -15.4 50.7 0 0 -1.8 TGTTCAGCTTTTTTTTTTTT

119 SEQ ID N0:829 -14.9 -20.2 64 -5.3 0 -4.3 TTATTCTTACAATATCCCTA

340 5EQ ID N0:830 -14.9 -19.9 60.5 -5 0 -2.6 TCTTGCATACGAATTACCTT

419 SEQ ID N0:831 -14.9 -21.5 62.7 -6.6 , 0 -5.1 TTCTTGCATACGAATTACCT

420 SEQ ID N0:832 -14.9 -21.5 62.7 -6.6 0 -5.1 TTATAAGTGACTCAAAGGTA

966 SEQ ID N0:833 -14.9 -17.5 55.7 -2.6 0 -3.5 CATTGTGCCATTAAAGTAGG

1349 SEQ ID N0:834 -14.9 -21 62.4 -6.1 0 -3.4 TGATGCTGCCGCAACTGCCT

1850 SEQ ID N0:835 -14.9 -29.5 77.4 -13 -1.6 -8 ATGCTTCTGAAGAACTTTCC

1917 SEQ ID N0:836 -14.9 -21.6 64.4 -6 -0.4 -8.3 AGCAGTCTGAATGATCGCAG

3019 SEQ ID N0:837 -14.9 -23.2 67.8 -7.6 -0.4 -6.3 TTCATGGATTTCTATAACTT

3575 SEQ ID N0:838 -14.9 -18.4 58.1 -3.5 0 -5 GTGGTTCCATTCCCATCTCT

3664 SEQ ID N0:839 -14.9 -28.6 81.6 -13 -0.4 -5.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GGCTGGGGTTGTTTATGAAT

3747 SEQ ID N0:840 -14.9 -23.8 69.9 -8.9 0 -3.7 GTGAGGCTGGGGTTGTTTAT

3751 SEQ ID N0:841 -14.9 -25.7 76.4 -10.8 0 -2.9 CTTCGGGTTCAGTTTCAGCT

3933 SEQ ID N0:842 -14.9 -26.2 77.3 -11.3 0 -4.3 CAATACTGTAGCAGGTTTTT

4059 SEQ ID N0:843 -14.9 -2l 64.1 -6.1 0.2 -4.5 CATATTCTAGCATGGTTTTG

4179 SEQ ID N0:844 -14.9 -20.7 63.6 -5.8 0 -5 GTTTACTTTCACGTTTTTCC

4649 SEQ ID N0:845 -14.9 -22.5 67.7 -7.6 0 -4.7 ATGTCTTGACCTCCAAACTT

4900 SEQ ID N0:846 -14.9 -23.2 66.9 -7.4 -0.7 -4.9 GTGTCTGAGGGAAACGAGCT

5198 SEQ ID N0:847 -14.9 -24.2 69.5 -9.3 0.6 -5.5 TGCGGATCCCCTTTGCTCCT

5379 SEQ ID N0:848 -14.9 -31.9 83.5 -16.3 -0.4 -7.5 TGGCATAGATAAACATGACC

5463 SEQ ID N0:849 -14.9 -20 59.3 -4.4 -0.4 -5.2 GTCATCCTCACTCAGGGGCT

5819 5EQ ID N0:850 -14.9 -29.2 84.3 -13.8 -0.2 -7.6 CATAAACCTGTCTTCCATCT

6089 SEQ ID N0:851 -14.9 -23.1 66.9 -8.2 0 -2 CTAGGTAGCCATTGGGTTTC

6711 SEQ ID N0:852 -14.9 -25.5 75.4 -l0 -0.3 -6.3 TCTAGGTAGCCATTGGGTTT

6712 SEQ ID N0:853 -14.9 -25.5 75.4 -10 -0.3 -6.3 AGACAGGGTTCTTGTATACT

6934 SEQ ID N0:854 -14.9 -22.3 68.4 -6.7 -0.4 -6.5 CCAAGACTTGTGAAGAATGG

7597 SEQ ID N0:855 -14.9 -20 59.3 -4.6 -0.2 -5.4 ATTTATATCATGTTAGGTAA

8304 SEQ ID N0:856 -14.9 -17.1 55.6 -2.2 0 -4.7 AATTTATATCATGTTAGGTA

8305 SEQ ID N0:857 -14.9 -17.1 55.6 -2.2 0 -4.7 TTTTTTTTCCACCTTATATA

9083 SEQ TD N0:858 -14.9 -20.1 61.4 -5.2 0 -3.2 TTCTTTCTCTGTGGATAAGA

51 SEQ ID N0:859 -14.8 -20.8 64.7 -5.2 -0.6 -3.9 TTTTTTTTTTTTTTGACCAC

104 SEQ ID N0:860 -14.8 -19 59.5 -4.2 0 -1.7 TCGGAAAATTGCCTTTCCTT

755 SEQ ID N0:861 -14.8 -23.3 65.2 -6 -2.5 -7.5 GGAGGGTTGCTCAAGGTCAT

904 SEQ ID N0:862 -14.8 -26.1 76.5 -9.3 -2 -4.7 CTTCTGGACACTGGCCTGCA

1497 SEQ ID N0:863 -14.8 -28.5 79.2 -13.7 0 -7.2 CTGTTGCTTTTTAAGCTGTT

1820 SEQ ID N0:864 -14.8 -22.3 67.7 -5.6 -1.9 -6.4 CCCACCTACTCCACTGAAAT

1883 SEQ ID N0:865 -14.8 -26 69.8 -11.2 0 -1.7 TTCAAATGTGCTGTGTTCAT

2258 SEQ ID N0:866 -14.8 -21.3 64.6 -6.5 0 -3.6 GCCTGACTAACGTTACTGTT

2335 SEQ ID N0:867 -14.8 -23.8 68.2 -8.5 0 -7.8 TCCTCCAGCATCTCCATTGA

2545 SEQ TD N0:868 -14.8 -28 78.5 -13.2 p -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCATGGGGTAGTGCTCCATG

2805 SEQ ID N0:869 -14.8 -26.6 77 -7.9 -3.9 -12.7 GATCGCAGTACAGACAATCC

3007 SEQ ID N0:870 -14.8 -23.3 67.1 -7.8 -0.4 -5.3 CAACACCAAGGTGAGGTTTC

3119 SEQ ID N0:871 -14.8 -23.1 67.2 -7.2 -0.8 -9.6 GCCAACACCAAGGTGAGGTT

3121 SEQ ID N0:872 -14.8 -26.4 73 -10 -l.3 -10.8 GAAACACTCCCGCATCTTAT

3530 SEQ ID N0:873 -14.8 -24 66.6 -9.2 0 -3.6 TTGCCTTCATGGATTTCTAT

3580 SEQ ID N0:874 -14.8 -23 68.2 -7.6 -0.3 -5 ATTGCCTTCATGGATTTCTA

3581 SEQ ID N0:875 -14.8 -23 68.2 -7.6 -0.3 -4.7 TTTGCTTATTTCAATTCCAG

3629 SEQ ID N0:876 -14.8 -20.5 62.4 -5.7 0 -3.6 ATGAAAGTCTCAAACCAGTT

4087 SEQ ID N0:877 -14.8 -19.6 59.2 -4.2 -0.3 -3.5 CAATGCACCACTACTGAGAA

4121 SEQ 2D N0:878 -14.8 -21.8 62.7 -7 0 -5.9 AAAGTTGGACATCCCAAAGA

5483 SEQ ID N0:879 -14.8 -20.9 60.5 -3.9 -2.2 -5.7 CTGGGTCGCATCGGGATCAA

5870 SEQ ID N0:880 -14.8 -26.8 73.3 -12 0 -6.9 AGGATCCAGGGCAGCTGCAA

5918 SEQ ID N0:881 -14.8 -28.3 78.5 -10.6 -l -14 CCTGCTTCACAGAGTTGCAG

6636 SEQ ID N0:882 -14.8 -26.1 75.5 -9.4 -1.9 -7.4 GTAGTTACAATGTTCACTTT

6750 SEQ ID N0:883 -14.8 -19.8 62.2 -5 0 -5.6 TATATTTTTCTAGAATGGAT

7257 SEQ ID N0:884 -14.8 -16.5 53.8 -1.l 0 -7.7 ATATATTTTTCTAGAATGGA

7258 SEQ ID N0:885 -14.8 -16.5 53.8 -1.1 0 -7.7 GATATATTTTTCTAGAATGG

7259 SEQ ID N0:886 -14.8 -16.5 53.8 -1.1 0 -7.7 ATTTAATAGAAGTTGTTTAT

7443 SEQ ID N0:887 -14.8 -15.2 51.1 0.4 0 -2.5 CTAGGTTTGAGTGTTTGACC

8093 SEQ ID N0:888 -14.8 -23.2 70.1 -7.9 -0.1 -3.8 TACTGCTTGGTGAATCATGC

8623 SEQ ID N0:889 -14.8 -22.8 67.4 -7.l , -0.8 -4.6 TAGTACTGCTTGGTGAATCA

8626 SEQ ID N0:890 -14.8 -21.9 66.3 -7.1 0 -6 GCTGCTTTTAATTTGCCTTT

8652 SEQ ID N0:891 -14.8 -23.8 69.3 -9 0 -52 TTTTTTTTTTTTTTTGACCA

105 SEQ ID N0:892 -14.7 -18.9 59.3 -4.2 0 -1.7 TGCCTTTCCTTTATTCATTA

746 SEQ TD N0:893 -14.7 -23.1 68.3 -8.4 0 -3 GAAAATTGCCTTTCCTTTAT

752 SEQ ID N0:894 -14.7 -20.7 60.9 -6 0 -3.2 CTGGACACTGGCCTGCATCT

1494 SEQ ID N0:895 -14.7 -28.4 78.7 -13.7 0 -72 AGTCTTGAGTCATGAGTCGA

1602 SEQ ID N0:896 -14.7 -23.1 70.4 -6.7 0 -11.6 AAATGTGCTGTGTTCATCAT

2255 SEQ ID N0:897 -14.7 -21.2 64.2 -6.5 0 -3.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGGAGTGGAAGAAGTCGTTC

3249 SEQ ID NO:898 -14.7 -22.1 66.5 -6.9 -0.2 -3.1 GGTTTGGCCAGCGACCTCCA

3326 SEQ ID N0:899 -14.7 -31.7 83.8 -15.7 -0.3 -10.6 TGGTTTGGCCAGCGACCTCC

3327 SEQ ID N0:900 -14.7 -31 82.6 -14.6 -l -11.4 TTGGAAACACTCCCGCATCT

3533 SEQ TD N0:901 -14.7 -25.5 69.4 -9.7 -1 -4.8 TCAGCTTGTTCACCTTCTCG

3919 SEQ ID NO:902 -14.7 -26 75.5 -11.3 0 -4.5 CCAGAATGAATATATAGGTA

4212 SEQ ID N0:903 -14.7 -17.7 55.2 -3 0 -4.2 CTGAGCTGCCAGGGTGAATT

5661 SEQ ID No:904 -14.7 -26.3 74.2 -11.1 0 -7.8 TCTGTATTCGAAGGGCATCC

6072 SEQ ID N0:905 -14.7 -24.7 70.9 -10 0 -7.2 CTTGTTTACGTTTCAAAGTG

6150 SEQ ID N0:906 -14.7 -19.4 59.6 -4.7 0 -4.8 TCTTTTTCTGGTTTGTCTTT

6418 SEQ ID N0:907 -14.7 -22.3 70.2 -7.6 0 -1.5 GTTGATACCCTGCTTCACAG

6644 SEQ ID NO:908 -14.7 -25.5 73 -9.9 -0.8 -3.6 GCAGATATGGAAGTTAAAAA

6807 SEQ ID N0:909 -14.7 -16.1 51.4 0 -1.3 -5.5 TTTAGATATATTTTTCTAGA

7263 SEQ TD N0:910 -14.7 -15.9 53.4 -1.1 0 -5.4 GTTTGAGTGTTTGACCAATG

8089 SEQ ID N0:911 -14.7 -21.4 64.4 -6.2 -0.2 -3.4 GTAGAAAATGATCTAGGTTT

8105 SEQ ID N0:912 -14.7 -17.6 56 -2.2 -0.4 -4.9 AGTTGGTAGAAAATGATCTA

8110 SEQ ID N0:913 -14.7 -17.5 55.7 -2.2 -0.2 -4.9 AAAGGTTATATTGAGGCAAC

8133 SEQ ID N0:914 -14.7 -18.4 56.9 -3.7 0 -4 TGCTTGGTGAATCATGCACT

8620 SEQ ID N0:915 -14.7 -23.8 69.2 -7.2 -1.9 -6.4 GAAGATGAGTTTAGATGCTT

8789 SEQ ID N0:916 -14.7 -19.6 60.9 -4.9 0 -3.6 ACGTGTAGCTTCTTGCATAC

429 SEQ ID N0:917 -14.6 -23.7 70.2 -7.5 -1.5 0 CTGTGCCATCTTTTCATCCT

461 SEQ ID N0:918 -14.6 -26.6 76.5 -l2 0 -3 CTGCAAACATTTATTCCTCA

1289 SEQ ID N0:919 -14.6 -21.3 62.7 -6.7 0 -4.9 TCATGTATGTTTTCCCAGCA

1656 SEQ ID N0:920 -14.6 -25.1 73.1 -10.5 0 -4.7 GAGCTTCTTCCTGTTGCTTT

1830 SEQ ID N0:921 -14.6 -26.3 78 -10.8 -0.7 -5.2 CCGCAACTGCCTGAGCTTCT

1842 SEQ ID N0:922 -14.6 -29.1 77.8 -13.3 -1.1 -5.7 GGAGTGGAAGAAGTCGTTCA

3248 SEQ ID N0:923 -14.6 -22.8 67.5 -6.9 -1.2 -4.1 AAGGCACATGGTTTGGCCAG

3335 SEQ ID N0:924 -14.6 -26.2 73.5 -8.4 -3.2 -9.6 CTTATTTCAATTCCAGTATT

3625 SEQ ID N0:925 -14.6 -19.5 60.5 -4.9 0 -2.5 CTCTTTGCTTTCTTCTAGTT

3845 SEQ TD N0:926 -14.6 -23 71.7 -8.4 0 -4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAGAAATCTAGCCAGCACCA

4285 SEQ ID N0:927 -14.6 -23.4 66 -8.8 0 -4.6 AGATGTCTTGACCTCCAAAC

4902 SEQ ID N0:928 -14.6 -22.8 66.2 -7.4 -0.6 -4.9 GAGGATCATGATGCTGATAT

5057 SEQ ID N0:929 -14.6 -21.1 63.6 -5.6 -0.6 -8.5 TGAGGATCATGATGCTGATA

5058 SEQ ID N0:930 -14.6 -21.1 63.5 -5.6 -0.7 -7.9 GATAAACATGACCAGGAAGA

5456 SEQ ID N0:931 -14.6 -19 56.9 -4.4 0 -5.2 TTGCCAAAGGTCTCAAAGTT

5542 SEQ ID N0:932 -14.6 -21.9 64.3 -7.3 0 -4 TCCTCACTCAGGGGCTCTGC

5815 SEQ ID N0:933 -14.6 -30 86 -13.3 -2.1 -7.7 GCATGGACCTCCTCTTGAAG

6547 SEQ ID N0:934 -14.6 -26 73.2 -11.4 0 -5.1 GAGACAGGGTTCTTGTATAC

6935 SEQ ID N0:935 -14.6 -22 67.8 -6.7 -0.4 -7.4 TACTTTAGATATATTTTTCT

7266 SEQ ID N0:936 -14.6 -16.4 54.4 -1.8 0 -4.3 CCCTGTTGGAAGCTTGCAAA

7490 SEQ ID N0:937 -14.6 -25.2 69.4 -10.1 -0.2 -7.2 TTGGTAGAAAATGATCTAGG

8108 SEQ ID N0:938 -14.6 -17.5 55.3 -2.2 -0.4 -4.9 TAGGTGGTTACTACTATTAT

8733 SEQ ID N0:939 -14.6 -19.7 62 -4.6 -0.1 -3.9 TATTGAAAGAAGATGAGTTT

8797 SEQ ID NO:940 -14.6 -15.5 51 -0.8 0 -2.3 TATATTGAAAGAAGATGAGT

8799 SEQ ID N0:941 -14.6 -l5 49.9 0 0 -2.2 TTCATCCTGCACATTTAATT

449 SEQ ID N0:942 -14.5 -21.2 63.2 -6.7 0 -4.8 CGGAAAATTGCCTTTCCTTT

754 SEQ ID N0:943 -14.5 -23 64.2 -6 -2.5 -7.5 TAAGCAAAACCCTCTTGCCA

992 SEQ ID N0:944 -14.5 -24.4 66.6 -7.9 -2 -6.2 CTGAGACATTGCCCAGGTCC

1092 SEQ ID N0:945 -14.5 -28.4 78.4 -13.2 -0.5 -4.4 AATCCATTGTGCCATTAAAG

1353 SEQ ID N0:946 -14.5 -20.6 60.4 -6.1 0 -3.1 AGCACGTAATGTCAACTGGT

1637 SEQ ID NO:947 -14.5 -22.8 66.3 -8.3 0 -4.8 TGAGCTTCTTCCTGTTGCTT

1831 SEQ ID N0:948 -14.5 -26.2 77.3 -10.8 -0.7 -5.2 CTCAACTTTGATGCTTCTGA

1927 SEQ ID N0:949 -14.5 -21.9 65.6 -7.4 0 -5 AGATCCAACATCCTTTGCCC

2219 SEQ ID N0:950 -14.5 -27.2 74.2 -12.2 -0.1 -4.5 TCCTGCTTTCGCCGTCTTCA

2274 SEQ ID N0:951 -14.5 -29.7 81.1 -14.5 -0.5 -3.6 ACTAACGTTACTGTTGCGTC

2330 SEQ ID N0:952 -14.5 -22.7 66.2 -7.2 -0.9 -7.8 GCATCACAGCAGTCCCAGAT

2686 SEQ ID N0:953 -14.5 -27.9 78.7 -12.9 -0.1 -4.2 TCTTATTGCCTTCATGGATT

3585 SEQ ID N0:954 -14.5 -23 68.2 -7.9 -0.3 -4.7 CATTCCCATCTCTAAGATAA

3657 SEQ ID N0:955 -14.5 -21.1 62.4 -5.9 -0.5 -3.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CACCTTCTCGGGGTAGAACA

3909 SEQ ID N0:956 -14.5 -25.7 72 -10.2 -0.9 -4.9 AGACGTAGGATTCGGCCAAT

5353 SEQ ID N0:957 -14.5 -24.6 68.3 -9.5 -0.3 -7.5 CATAGATAAACATGACCAGG

5460 5EQ ID N0:958 -14.5 -18.9 56.9 -4.4 0 -5.2 CAGATGGGTCCCCACGGTCT

5688 SEQ ID N0:959 -14.5 -30.7 82.2 -15.3 -0.6 -8.8 ACTTTTGATTTTCTCTGACC

6453 SEQ ID N0:960 -14.5 -21.7 65.9 -7.2 0 -2.4 GACACATATACTTTACCTTC

6520 SEQ ID N0:961 -14.5 -20.4 62.1 -5.9 0 -2.4 GTTGACACATATACTTTACC

6523 SEQ ID N0:962 -14.5 -20.3 61.7 -5.8 0 -2.9 AAATTTATATCATGTTAGGT

8306 SEQ ID N0:963 -14.5 -16.7 54.2 -2.2 0 -4.7 AGATGAGTTTAGATGCTTAT

8787 SEQ ID N0:964 -14.5 -19.4 61.1 -4.9 0 -3.6 TTTTTTTTCTTTCTTTCTCT

61 SEQ ID N0:965 -14.4 -20.3 65.4 -5.9 0 0 AGGGTATAACGTGTCTTCCT

147 SEQ ID N0:966 -14.4 -24.4 71.2 -10 0 -5.3 GTATCTCATCCCTGTCAAAC

265 SEQ ID N0:967 -14.4 -23.7 69.4 -9.3 0 -1.8 TTTTTGGGCTTCTTGGCTTT

568 SEQ ID N0:968 -14.4 -25.1 74.3 -9.7 -0.9 -3.7 TTTATAAGTGACTCAAAGGT

967 SEQ ID N0:969 -14.4 -17.9 56.6 -3.5 0 -4.7 CACTGCAAACATTTATTCCT

1291 SEQ ID N0:970 -14.4 -21.1 61.9 -6.7 0 -4.9 GGACACTGGCCTGCATCTGA

1492 SEQ ID N0:971 -14.4 -28.1 78.1 -13.7 0 -7.2 TAGTCTTGAGTCATGAGTCG

1603 SEQ ID N0:972 -14.4 -22.2 68.3 -6.1 0 -11.5 GGGAGCCACGGATACTCAAG

2148 SEQ ID N0:973 -14.4 -25.5 70.7 -9.8 -1.2 -4.5 GTCTGTGCGGCACAAACAGT

2301 SEQ ID N0:974 -14.4 -25.6 72.2 -8.6 -1.4 -13.2 TAACGTTACTGTTGCGTCGC

2328 SEQ ID N0:975 -14.4 -24.2 68.1 -8.8 -0.9 -7.6 TCAGTCATGGGGTAGTGCTC

2809 SEQ ID N0:976 -14.4 -26.2 79.5 -11.8 0 -4.l TGCCTTCATGGATTTCTATA

3579 SEQ ID N0:977 -14.4 -22.6 67.3 -7.6 -0.3 -5 ATCTTATTGCCTTCATGGAT

3586 SEQ ID N0:978 -14.4 -22.9 67.8 -7.9 -0.3 -4.7 TCTTTGCTTTCTTCTAGTTC

3844 SEQ ID N0:979 -14.4 -22.5 71.4 -8.1 0 -4 GTTACCCGTTGTCATGTTAA

4565 SEQ ID N0:980 -14.4 -23.9 68.9 -9.5 0 -4.7 TGACCTCCAAACTTCTTTTT

4894 SEQ ID N0:981 -14.4 -22.3 65 -7.9 0 -2.1 GAGGTATGGGTTTCTGAGGT

4980 SEQ ID N0:982 -14.4 -24.8 75.1 -10.4 0 -2.8 TGATCAGACGTAGGATTCGG

5358 SEQ ID N0:983 -14.4 -22.5 65.4 -7.6 -0.2 -6.6 ATAGATAAACATGACCAGGA

5459 SEQ ID N0:984 -14.4 -18.8 56.9 -4.4 0 -5.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCTGTTTTTTCTGGAGTGGA

6328 SEQ ID N0:985 -14.4 -23.1 71 -87 0 -2.7 CTCCCATCTGTTTTTTCTGG

6334 SEQ ID N0:986 -14.4 -25.5 74.4 -11.1 0 -3.2 TAGTCTAGGTAGCCATTGGG

6715 SEQ ID N0:987 -14.4 -25 74.3 -10 -0.3 -6.3 ATACATCCCTGTTGGAAGCT

7496 SEQ ID N0:988 -14.4 -25 71.1 -9.9 -0.5 -6.8 GACATAATGGACAGAGCAGG

7655 SEQ ID N0:989 -14.4 -21.5 63.6 -7.1 0 -4.1 ATAGTTGGTAGAAAATGATC

8112 SEQ ID N0:990 -14.4 -16.6 53.7 -2.2 0 -3.9 TATTTGTAATAGCTTTAGTG

8232 SEQ ID N0:991 -14.4 -17.6 57 -3.2 0 -4.6 CTGTAGTACTGCTTGGTGAA

8629 SEQ ID N0:992 -14.4 -22.9 68.7 -8 0 -7.8 CTGCTTATGCCTCCGCTTCG

3 SEQ ID N0:993 -14.3 -29.3 78.2 -14.1 -0.8 -5 TTTTTCTTTCTTTCTCTGTG

58 SEQ ID N0:994 -14.3 -21.2 67.5 -6.9 0 0 TTTTTTTCTTTCTTTCTCTG

60 SEQ ID N0:995 -14.3 -20.2 64.8 -5.9 0 0 GCAAACATTTATTCCTCAGA

1287 SEQ ID N0:996 -14.3 -21 62.4 -6.7 0 -3.4 AAATATCATGTATGTTTTCC

1661 SEQ ID N0:997 -14.3 -18.2 57.2 -3.9 0 -4.9 GGTGGCCTGATTCTGCTCCT

1754 SEQ ID N0:998 -14.3 -30.3 84.8 -15.4 -0.3 -7.2 CAGGGAGCCACGGATACTCA

2150 SEQ ID N0:999 -14.3 -26.9 74 -11.3 -1.2 -4.5 TGCTGTGTTCATCATCAGCA

2250 SEQ ID N0:1000 -14.3 -25 74.7 -6.5 -4.2 -9.6 TAAGGCACATGGTTTGGCCA

3336 SEQ ID N0:1001 -14.3 -25.9 72.7 -8.4 -3.2 -9.1 GGTTTCCAATGACCATGACC

3369 SEQ ID N0:1002 -14.3 -25.3 70.3 -10.1 -0.7 -5 CAATTCCAGTATTATTGGAC

3618 SEQ ID N0:1003 -14.3 -20 60.8 -4.2 -1.4 -7.2 CTCTTTGCTTATTTCAATTC

3632 SEQ ID N0:1004 -14.3 -20 62.5 -5.7 0 -3.6 AGCTCTTTGCTTATTTCAAT

3634 SEQ ID N0:1005 -14.3 -21.3 65.2 -5.7 , -1.2 -4.3 TTTTTCGAAGATTCCACCAG

4044 SEQ ID N0:1006 -14.3 -22.2 64.2 -7.2 -0.4 -6.8 GGTTTTTCGAAGATTCCACC

4046 SEQ TD N0:1007 -14.3 -23.9 68.3 -8.9 -0.4 -6.6 ACCACTACTGAGAAGGATCA

4115 SEQ ID N0:1008 -14.3 -22.2 65.1 -7 -0.8 -6.3 CACCACTACTGAGAAGGATC

4116 SEQ ID N0:1009 -14.3 -22.2 65.1 -7 -0.8 -5.l GGGAAACGAGCTTCAGCACA

5190 SEQ ID N0:1010 -14.3 -24.5 68.6 -9.3 -0.7 -6.6 AGCGTGCGGATCCCCTTTGC

5383 SEQ ID N0:1011 -14.3 -31.5 82.2 -16.5 -0.4 -7.5 CTGTTGCCAAAGGTCTCAAA

5545 SEQ ID N0:1012 -14.3 -22.7 65.5 -8.4 0 -3.6 CCAGCCAGCAGAGGTTGTAA

5585 SEQ ID N0:1013 -14.3 -27 75.9 -11.8 -0.8 -5.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GAGCTGCCAGGGTGAATTGT

5659 SEQ ID N0:1014 -14.3 -26.6 75.6 -12.3 0 -5.6 TGAGCTGCCAGGGTGAATTG

5660 SEQ ID N0:1015 -14.3 -25.4 72.1 -11.1 0 -5.6 CCTCACTCAGGGGCTCTGCA

5814 5EQ ID N0:1016 -14.3 -30.3 85 -14.4 -1.6 -5.1 AACTGGGTCGCATCGGGATC

5872 SEQ ID N0:1017 -14.3 -26.3 72.8 -12 0 -6.9 TATAGTCTAGGTAGCCATTG

6717 SEQ ID N0:1018 -14.3 -22.3 68 -7.4 -0.3 -4 AGGCTATATATATATTTGCC

7422 SEQ ID N0:1019 -14.3 -20.4 62.2 -4 -1.3 -12.3 CAGTAGGCAGTATCCAGTGT

7772 SEQ ID N0:1020 -14.3 -25.7 76.9 -11.4 0.6 -4 CACTTTGTTCATGGCTAAAA

8270 SEQ ID N0:1021 -14.3 -19.9 59.9 -5.6 0 -4.7 TGGTGAATCATGCACTAGTT

8616 SEQ ID N0:1022 -14.3 -22 66 -6.5 -1.1 -6.2 GCACGTTTTTCGATAGCAGC

538 SEQ ID NO:1023 -14.2 -24.9 71.1 -9.6 -1 -5.6 AAAATTGCCTTTCCTTTATT

751 SEQ ID N0:1024 -14.2 -20.2 60 -6 0 -3.2 TCTAAGCAAAACCCTCTTGC

994 SEQ ID N0:1025 -14.2 -23 65.3 -7.9 -0.8 -5 CAGTTCCATGGATCACGAAG

1027 SEQ ID N0:1026 -14.2 -22.9 65.7 -7.4 0 -10.5 GTTTCAAAAGCAGAATCGCT

1318 SEQ ID N0:1027 -14.2 -20.7 60.9 -5.7 -0.6 -7 GTTGGTTTCAAAAGCAGAAT

1322 SEQ ID N0:1028 -14.2 -19.3 58.9 -4.3 -0.6 -1.1 TGTTGGTTTCAAAAGCAGAA

1323 SEQ TD N0:1029 -14.2 -19.3 58.8 -4.3 -0.6 -1.1 TGGTGTTGGTTTCAAAAGCA

1326 SEQ ID N0:1030 -14.2 -21.8 64.9 -6.9 -0.4 -4.2 TCTGGACACTGGCCTGCATC

1495 SEQ ID N0:1031 -14.2 -27.9 78.6 -13.7 0 -7.2 ATAGTCTTGAGTCATGAGTC

1604 SEQ ID N0:1032 -14.2 -21.4 68.2 -6.1 0 -10.1 AATAGTCTTGAGTCATGAGT

1605 SEQ ID N0:1033 -14.2 -20.3 64 -6.1 0 -7.3 GCACGTAATGTCAACTGGTA

1636 SEQ ID N0:1034 -14.2 -22.5 65.5 -8.3 0 -4.8 GCCATGGCCACCACAGCCAG

1729 SEQ ID N0:1035 -14.2 -32.5 84 -15.5 -2.8 -11.7 TTTTAAGCTGTTCGAGCATC

1812 SEQ ID N0:1036 -14.2 -22 66 -5.5 -2.3 -7.3 GTTGCTTTTTAAGCTGTTCG

1818 SEQ TD N0:1037 -14.2 -22.6 67.6 -5.8 -2.6 -7.1 TCTTCCTGTTGCTTTTTAAG

1825 SEQ ID N0:1038 -14.2 -22.1 67.1 -7.9 0 -3.6 CTCCTAACCCACCTACTCCA

1890 SEQ ID N0:1039 -14.2 -29.1 77 -14.9 0 -0.3 TGTCTTCAGATTCGGATTTG

2043 SEQ ID N0:1040 -14.2 -21.6 65.4 -7.4 0 -4.4 CTGTCTTCAGATTCGGATTT

2044 SEQ ID N0:1041 -14.2 -22.5 67.6 -7.4 -0.7 -5 ATCCTCCAGCATCTCCATTG

2546 SEQ ID N0:1042 -14.2 -27.4 77.1 -13.2 0 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CGCAGTACAGACAATCCCTC

3004 SEQ ID N0:1043 -14.2 -25.6 71.3 -10.7 -0.4 -5.3 GTCTGAATGATCGCAGTACA

3015 SEQ ID N0:1044 -14.2 -22.5 66.3 -7.6 -0.4 -6.3 TTTCAATTCCAGTATTATTG

3621 SEQ ID N0:1045 -14.2 -18.6 58.4 -4.4 0 -3.5 CCCTTTGCCTTCTTCTGTAC

4019 SEQ ID N0:1046 -14.2 -27.7 78.7 -13.5 0 -3.8 ATTTCCAGAATGAATATATA

4216 SEQ ID NO:1047 -14.2 -16.2 52.2 -2 0 -5.3 TCAAAGTTTACTTTCACGTT

4654 SEQ ID N0:1048 -14.2 -19.5 59.9 -3.9 -1.3 -8.7 ATAAAGATGTCTTGACCTCC

4906 SEQ ID N0:1049 -14.2 -21.6 63.9 -7.4 0.2 -6.8 CGAAGGGCATCCATCTCTCC

6064 SEQ ID N0:1050 -14.2 -27.7 76.1 -12.3 -1.1 -4.3 GTATTCGAAGGGCATCCATC

6069 SEQ ID N0:1051 -14.2 -24.5 70.2 -9.1 -1.1 -8.2 ATATGGCAGATATGGAAGTT

6812 SEQ ID N0:1052 -14.2 -20.1 61.2 -4.5 -1.3 -4.7 AACATGCATTAGTGATAGCC

7545 SEQ ID N0:1053 -14.2 -21.8 64.5 -7.6 0 -6.8 TTGTGAAGAATGGATTGAGT

7590 SEQ ID NO:1054 -14.2 -18.8 58.3 -4.6 0 -2.9 TAGAAAATGATCTAGGTTTG

8104 SEQ ID N0:1055 -14.2 -16.4 53.1 -2.2 0 -4.9 TAAAAGGTTATATTGAGGCA

8135 SEQ ID N0:1056 -14.2 -17.9 55.9 -3.7 0 -4 GCCCAATAAGTGAAATGCAA

8480 SEQ ID N0:1057 -14.2 -2l 59.7 -6.8 0 -5.6 TTTCTTTCTCTGTGGATAAG

52 SEQ ID N0:1058 -14.1 -20.3 63.6 -6.2 0 -2.9 TTTTTTTTTTTTTTTTGACC

106 SEQ ID N0:1059 -14.1 -18.3 58.3 -4.2 0 -O.l GTGTAGCTTCTTGCATACGA

427 SEQ ID N0:1060 -14.1 -24.1 71 -8.2 -1.8 -5.2 TCATCCTGCACATTTAATTA

448 SEQ ID N0:1061 -14.1 -20.8 62.3 -6.7 0 -4.8 TTTTCATCCTGCACATTTAA

451 SEQ ID N0:1062 -14.1 -21.3 63.6 -7.2 0 -4.8 ACTGCAAACATTTATTCCTC

1290 SEQ ID N0:1063 -14.1 -20.8 62.1 -6.7 0 -4.9 TTCGAGCATCTGCTGAAATT

1802 SEQ ID N0:1064 -14.1 -21.7 63.5 -6.3 -1.2 -8.9 ACGCTGTCTTCAGATTCGGA

2047 SEQ ID N0:1065 -14.1 -25.1 71.8 -9.5 -l.4 -6.1 CCGTCTTCAAATGTGCTGTG

2263 SEQ ID N0:1066 -14.1 -24.2 69 -10.1 0 -3.6 CCCATTTGCTGGAAGCCCTG

2378 SEQ ID N0:1067 -14.1 -29.4 77.4 -13.1 -2.2 -5.8 CGGAACACAATCAGGAAGGA

3265 SEQ ID N0:1068 -14.1 -21 60.4 -6.9 0 -2.9 TATCTTATTGCCTTCATGGA

3587 SEQ ID N0:1069 -14.1 -22.6 67.3 -7.9 -0.3 -4.7 CCATTCCCATCTCTAAGATA

3658 SEQ ID N0:1070 -14.1 -23.8 68.1 -9 -0.5 -3.9 AGGCTGGGGTTGTTTATGAA

3748 SEQ ID N0:1071 -14.1 -23.8 70.2 -9.7 0 -3.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTCACCTTCTCGGGGTAGAA

3911 SEQ ID N0:1072 -14.1 -25.3 72.3 -9.7 -1.4 -5.4 GTTTAAAGTCTTCTTCGGGT

3945 SEQ ID N0:1073 -14.1 -22.5 67.9 -8.4 0 -4.7 ACAATACTGTAGCAGGTTTT

4060 SEQ ID N0:1074 -14.1 -21.1 64.3 -6.1 -0.7 -5.3 TTTCCAGAATGAATATATAG

4215 SEQ ID N0:1075 -14.1 -16.2 52.3 -2.1 0 -4.2 ATCAAAGTTTACTTTCACGT

4655 SEQ ID N0:1076 -14.1 -19.4 59.6 -3.9 -1.3 -7.1 TAAAGATGTCTTGACCTCCA

4905 SEQ ID N0:1077 -14.1 -22.3 65.1 -7.4 -0.6 -6.8 AGAGCAGCGTGCGGATCCCC

5388 SEQ ID N0:1078 -14.1 -31.7 82.7 -15.4 -2.2 -11.1 AAGTTGGACATCCCAAAGAT

5482 SEQ ID N0:1079 -14.1 -21.6 62.4 -5.3 -2.2 -5.8 AGGCAAAGTTGGACATCCCA

5487 SEQ ID N0:1080 -14.1 -25.4 70.8 -10.4 -0.8 -5.6 TTTCAAAGTGGTTGTAATAG

6140 SEQ ID N0:1081 -14.1 -17.5 56 -2.7 -0.4 -3.8 CAATCCTCCCTTTAATTGCC

6261 5EQ ID N0:1082 -14.1 -25.7 70.5 -11.6 0 -3.3 TCCCATTTAGTTTGTCAATA

6306 SEQ ID NO:1083 -14.1 -21.7 65.1 -7.6 0 -2.9 CTCTGACCTCTTTTCCTTTG

6441 SEQ ID N0:1084 -14.1 -25 73.1 -10.9 0 -2.4 ACTTCACTGTCTTGTATAGG

6603 SEQ ID N0:1085 -14.1 -21.8 67.6 -7.7 0 -2.4 ATCAGCTTTGCTGAGAGCAG

7338 SEQ ID N0:1086 -14.1 -24.4 72.6 -6.7 -3.6 -11.8 ACATAATGGACAGAGCAGGT

7654 SEQ ID N0:1087 -14.1 -22.1 65.4 -8 0 -4.1 GTAGGCAGTATCCAGTGTGT

7770 SEQ ID N0:1088 -14.1 -26.2 79.1 -11.6 -0.2 -4 TTTTTTTCCACCTTATATAA

9082 SEQ ID N0:1089 -14.1 -19.3 59.1 -5.2 0 -5 GAGACCTTTCCAGAATCCTC

24 SEQ ID NO:1090 -14 -25.2 72 -11.2 0 -2.9 TTTTTTTTTTTTGACCACAG

102 SEQ ID N0:1091 -14 -19.5 60.3 -5.5 0 -2.3 CTGTTCAGCTTTTTTTTTTT

120 SEQ ID N0:1092 -14 -21 65.7 -7 0 -4.5 AGGGTTGCTCAAGGTCATAA

902 SEQ ID N0:1093 -14 -23.3 69.2 -9.3 0 -3.6 GAGGGTTGCTCAAGGTCATA

903 SEQ ID N0:1094 -14 -24.6 73.1 -9.3 -1.2 -4.1 CAGCACGTAATGTCAACTGG

1638 SEQ ID NO:1095 -14 -22.3 64.4 -8.3 0 -4.8 GGAACTCAACTTTGATGCTT

1931 SEQ ID N0:1096 -14 -21.3 63.3 -7.3 0 -4.8 TGCTCTCTCTGTCTTCTTTT

1984 SEQ ID N0:1097 -14 -24.8 77.1 -10.8 0 -3.6 GTGTTCATCATCAGCAAAGT

2246 SEQ ID N0:1098 -14 '22.1 67.1 -8.1 0 -4.1 CTGTGCATCTTCCCATTTGC

2389 SEQ ID N0:1099 -14 -27.1 77 -12.4 -0.4 -5.4 GAATCCTCCAGCATCTCCAT

2548 SEQ ID N0:1100 -l4 -27.2 75.8 -13.2 0 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GGAATCCTCCAGCATCTCCA

2549 SEQ ID N0:1101 -14 -28.4 78.4 -13.2 -1.1 -6.5 GCTCACGGCTCTTTGCCTTC

2573 SEQ ID N0:1102 -14 -29.4 82.4 -12.7 -2.7 -7.5 TTGACAGACCAAGCTCCATT

2979 SEQ ID N0:1103 -14 -24.3 68.9 -10.3 0 -5 TTTGACAGACCAAGCTCCAT

2980 SEQ ID N0:1104 -14 -24.3 68.9 -10.3 0 -5 ATTTGACAGACCAAGCTCCA

2981 SEQ ID N0:1105 -14 -24.3 68.9 -10.3 0 -5 CATTTGACAGACCAAGCTCC

2982 SEQ ID N0:1106 -14 -24.3 68.9 -10.3 0 -5 CAGTCTGAATGATCGCAGTA

3017 SEQ ID N0:1107 -14 -22.3 66 -7.6 -0.4 -6.3 CACAAGGTTTCCAATGACCA

3374 SEQ ID N0:1108 -14 -23.4 66.1 -8.5 -0.7 -5 GCAAGGTTGTCTGAGCTAAA

3430 SEQ ID N0:1109 -14 -22.4 66.4 -7.9 -0.1 -5.5 TTATTGCCTTCATGGATTTC

3583 SEQ ID N0:1110 -14 -22.2 66.6 -7.6 -0.3 -4.7 AATGAAAGTCTCAAACCAGT

4088 SEQ ID NO:llll -14 -18.8 57 -4.2 -0.3 -3.4 GGTATGGGTTTCTGAGGTTT

4978 SEQ ID N0:1112 -14 -24.4 74.1 -10.4 0 -2.8 CGAGGTATGGGTTTCTGAGG

4981 SEQ ID N0:1113 -14 -24.4 71.3 -10.4 0 -2.8 TCATGTATTTGCCCTGGTCA

5115 SEQ ID N0:1114 -14 -26.4 75.8 -12.4 0 -4.7 AGTGTCTGAGGGAAACGAGC

5199 SEQ ID N0:1115 -14 -23.3 67.8 -9.3 3 -3.5 ATAAACATGACCAGGAAGAG

5455 SEQ ID N0:1116 -14 -18.4 55.8 -4.4 0 -4.3 AGATAAACATGACCAGGAAG

5457 SEQ 2D NO:lll7 -14 -18.4 55.8 -4.4 0 -5.2 GATGGCATAGATAAACATGA

5465 SEQ ID N0:1118 -14 -18.4 56.4 -4.4 0 -5.2 TCGCATCGGGATCAAACTTT

5865 SEQ ID N0:1119 -14 -22.9 64.7 -8.9 0 -6.9 GTAATAGGCTCATAAGAGAC

6127 SEQ ID N0:1120 -14 -19.3 60 -3.9 -1.3 -4.7 TCAAAGTGGTTGTAATAGGC

6138 SEQ ID N0:1121 -14 -20.3 62.1 -5.6 -0.4 -3.8 GGACCTCCTCTTGAAGTCCA ' 6543 SEQ ID N0:1122 -14 -27.8 77.8 -11.4 -2.4 -6.5 CATGGACCTCCTCTTGAAGT

6546 SEQ ID NO:ll23 -14 -25.4 72.3 -11.4 0 -5.1 CTAAAGGTGTTTGGTGTAGT

6765 SEQ ID N0:1124 -14 -21.5 66.1 -7.5 0 -2.2 GCTTTGCTGAGAGCAGAAGA

7334 SEQ ID N0:1125 -14 -23.8 70 -6.7 -3.1 -8.6 CTATCAGCTTTGCTGAGAGC

7340 SEQ ID N0:1126 -14 -24.3 72.5 -6.7 -3.6 -11.3 CAAGACTTGTGAAGAATGGA

7596 SEQ ID N0:1127 -14 -18.6 56.9 -4.6 0.6 -4.1 CCCATTATCTAACTTTTATT

8017 SEQ ID N0:1128 -14 -20 60.3 -6 0 -1.6 TTTGAGTGTTTGACCAATGT

8088 SEQ ID N0:1129 -14 -21.4 64.4 -6.9 -0.2 -3.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Tntra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATATTTGTAATAGCTTTAGT

8233 SEQ ID N0:1130 -l4 -17.6 57 -3.6 0 -4.6 TTTTCCACCTTATATAATTT

9079 SEQ ID N0:1131 -14 -19.2 58.8 -5.2 0 -5.3 TTTTTCCACCTTATATAATT

9080 SEQ ID N0:1132 -14 -19.2 58.8 -5.2 0 -5.3 TTTTTTCCACCTTATATAAT

9081 SEQ ID N0:1133 -14 -19.2 58.8 -5.2 0 -5.3 TTTTTTCTTTCTTTCTCTGT

59 SEQ ID N0:1134 -13.9 -21.3 68.1 -7.4 0 0 TCAGCTTTTTTTTTTTTTTT

116 SEQ ID NO:1135 -13.9 -19.2 61.4 -5.3 0 -4.5 TTCAGCTTTTTTTTTTTTTT

117 SEQ ID N0:1136 -13.9 -19.2 61.4 -5.3 0 -4.5 GCTTCTTGCATACGAATTAC

422 5EQ ID N0:1137 -13.9 -21.3 63 -6.5 -0.7 -5.1 CATCATTATCTTGTTCCTTT

585 5EQ ID N0:1138 -13.9 -21.8 66.5 -7.9 0 -1.9 TCTTATTGATATAGTAGGGA

714 SEQ ID N0:1139 -13.9 -19.1 60.6 -5.2 0 -2.7 GGAAAATTGCCTTTCCTTTA

753 SEQ TD NO:1140 -13.9 -21.9 63.3 -6 -2 -7.2 CTGCATCTGAGCCATTTCCA

1482 SEQ ID N0:1141 -13.9 -27.1 76 -12.7 -0.1 -4.9 ACACTGGCCTGCATCTGAGC

1490 SEQ ID NO:ll42 -13.9 -28.1 78.9 -14.2 0 -6.5 TTCTGGACACTGGCCTGCAT

1496 SEQ ID N0:1143 -13.9 -27.6 77.2 -13.7 0 -7.2 ATCATGTATGTTTTCCCAGC

1657 SEQ ID N0:1144 -13.9 -24.4 71.9 -10.5 0 -4.7 TAGGCCATGGCCACCACAGC

1732 SEQ ID NO:ll45 -13.9 -30.7 81.7 -12.2 -2.5 -17.4 ACTCCACTGAAATCTCTTGA

1876 SEQ ID N0:1146 -13.9 -21.8 64.4 -7.9 0 -2.5 TGGAACTCAACTTTGATGCT

1932 SEQ ID N0:1147 -13.9 -21.2 62.8 -7.3 0 -5 TTTGCCCGACCTCTGAAACT

2206 SEQ ID N0:1148 -13.9 -26.1 70.1 -12.2 0 -3 CATCAGTAGCAGCAAGGTTG

3441 SEQ ID N0:1149 -13.9 -23.5 70 -9.6 0 -5.4 TGGAAACACTCCCGCATCTT

3532 SEQ ID N0:1150 -13.9 -25.5 69.4 -10.5 -l -4.8 TCACAACCACCCTCATGCCT

4428 SEQ ID N0:1151 -13.9 -29.1 76.9 -15.2 0 -4.4 CAAAGTTTACTTTCACGTTT

4653 SEQ ID N0:1152 -13.9 -19.2 58.9 -3.9 -1.3 -8.7 AGGTATGGGTTTCTGAGGTT

4979 SEQ ID N0:1153 -13.9 -24.3 74 -10.4 0 -2.8 GGGTCATGTATTTGCCCTGG

5118 SEQ ID N0:1154 -13.9 -27.7 78,2 -12.3 -1.4 -6.9 ATGACCAGGAAGAGCAGGAG

5449 SEQ TD N0:1155 -13.9 -23.5 67.9 -9.6 0 -4.1 GATCCAGGGCAGCTGCAAAA

5916 SEQ ID NO:ll56 -13.9 -25.7 70.9 -8.9 -1 -14 TCGAAGGGCATCCATCTCTC

6065 SEQ ID N0:1157 -13.9 -26.1 74.2 -11.3 -0.8 -6.3 TAGGCTCATAAGAGACTTTG

6123 SEQ 2D N0:1158 -13.9 -20.2 62.1 -4.9 -1.3 -5.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol O

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CCCATTTAGTTTGTCAATAA

6305 SEQ ID N0:1159 -13.9 -20.6 61.5 -6.7 0 -2.9 ACTGTCTTGTATAGGCACTG

6598 SEQ ID N0:1160 -13.9 -23.1 69.9 -8.5 -0.5 -4 TTAGATATATTTTTCTAGAA

7262 SEQ ID N0:1161 -13.9 -15.1 51.1 -1.1 0 -7.4 TACAGGATACATCCCTGTTG

7502 SEQ ID N0:1162 -13.9 -23.6 68.4 -6.7 -3 -8.4 ATACAGGATACATCCCTGTT

7503 SEQ ID N0:1163 -13.9 -23.6 68.5 -6.7 -3 -8.4 CTTGTGAAGAATGGATTGAG

7591 SEQ ID N0:1164 -13.9 -18.5 57.3 -4.6 0 -2.9 ACCAAGACTTGTGAAGAATG

7598 SEQ ID N0:1165 -13.9 -19 57.4 -4.6 -0.2 -5.4 ACATTCTTTAACCAAGACTT

7608 SEQ ID NO:1166 -13.9 -19.5 59.2 -4.6 -0.9 -3.4 CATTTATTCTTACAATATCC

343 SEQ ID N0:1167 -13.8 -18.1 56.9 -4.3 0 -2.6 GGGATCCAGGTCCTCCAGGG

698 SEQ ID N0:1168 -13.8 -31.3 86.2 -15.5 -2 -10.3 ATCGGAAAATTGCCTTTCCT

756 SEQ ID N0:1169 -13.8 -23.2 64.8 -6.9 -2.5 -7.5 GAGACATTGCCCAGGTCCAC

1090 SEQ ID N0:1170 -13.8 -28.4 78.4 -13.9 -0.5 -4.4 TGGACACTGGCCTGCATCTG

1493 SEQ ID NO:ll71 -13.8 -27.5 76.6 -13.7 0 -7.2 TCCTTCTGGACACTGGCCTG

1499 SEQ ID N0:1172 -13.8 -28.4 79 -13.7 -0.8 -8.8 ACCCACCTACTCCACTGAAA

1884 SEQ ID N0:1173 -13.8 -26.2 70.3 -12.4 0 -1.7 ACAGGGAGCCACGGATACTC

2151 SEQ ID NO:1174 -13.8 -26.4 73.5 -l2 -0.3 -4.3 TGCTATTGCGTCTTGGGGAA

2172 SEQ ID N0:1175 -13.8 -25.2 71.5 -9.8 -1.5 -6.6 ATGCTTGTTTTGCTATTGCG

2182 SEQ ID N0:1176 -13.8 -23.4 68.3 -8 -1.5 -5.8 ATGTCTGTGCGGCACAAACA

2303 SEQ ID N0:1177 -13.8 -24.4 68.6 -8.6 0.6 -12.1 AACGTTACTGTTGCGTCGCT

2327 SEQ TD N0:1178 -13.8 -25.4 70.5 -10.6 -0.9 -7.4 TCTCTTTGCTTTCTTCTAGT

3846 SEQ ID N0:1179 -13.8 -23.3 73.2 -9.5 0 -4 CAAACATGTTACCCGTTGTC

4572 SEQ ID N0:1180 -13.8 -23.1 65.6 -8.6 0 -9 CCAACATTATCAAAGTTTAC

4663 SEQ ID N0:1181 -13.8 -17.7 55 -3.9 0 -4.1 ATTTTCTTCATATACAGGCT

4775 SEQ ID N0:1182 -13.8 -21 64.7 -7.2 0 -3.7 TCCTTGGAATTTGTTTGCTG

5003 SEQ ID N0:1183 -13.8 -22.8 67.2 -9 0 -4.3 AGGGTCATGTATTTGCCCTG

5119 SEQ ID N0:1184 -13.8 -26.5 75.9 -10.5 -2.2 -8.4 TTGATCAGACGTAGGATTCG

5359 SEQ ID NO:ll85 -13.8 -21.4 63.3 -7.6 0 -6.6 GCGTGCGGATCCCCTTTGCT

5382 SEQ ID N0:1186 -13.8 -32.4 83.6 -17.9 -0.4 -7.5 GCCAAAGGTCTCAAAGTTGA

5540 SEQ ID N0:1187 -13.8 -22.4 65.2 -8.6 0 -5.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGTAATAGGCTCATAAGAGA

6128 SEQ ID N0:1188 -13.8 -19.1 59.3 -3.9 -1.3 -4.7 TACGTTTCAAAGTGGTTGTA

6144 SEQ ID N0:1189 -13.8 -20.4 62.1 -5.9 -0.4 -5.6 AAGGTGTTTGGTGTAGTTAC

6762 SEQ ID N0:1190 -13.8 -21.6 67.5 -7.8 0 -2.2 GGAGACTTTACAGGCACATA

7114 SEQ ID N0:1191 -13.8 -22.6 67 -8.8 0 -4 GAGGAGACTTTACAGGCACA

7116 SEQ TD N0:1192 -13.8 -23.5 69.2 -8.8 -0.7 -5.2 AGCTTTGCTGAGAGCAGAAG

7335 SEQ ID N0:1193 -13.8 -23.2 68.9 -6.7 -2.7 -10.2 TATCAGCTTTGCTGAGAGCA

7339 SEQ ID NO:ll94 -13.8 -24.1 71.7 -6.7 -3.6 -11.8 GGCTATATATATATTTGCCC

7421 SEQ TD N0:1195 -13.8 -22.4 65.7 -6.9 -0.9 -11.5 AAAAGGTTATATTGAGGCAA

8134 SEQ ID N0:1196 -13.8 -17.5 54.6 -3.7 0 -4 TATATTTGTAATAGCTTTAG

8234 SEQ ID NO:1197 -13.8 -16.1 53.3 -2.3 0 -4.6 CAATAAGTGAAATGCAATAA

8477 SEQ ID N0:1198 -13.8 -14.2 47.2 0 0 -5.6 AAGATGAGTTTAGATGCTTA

8788 SEQ ID N0:1199 -13.8 -18.7 59 -4.9 0 -3.6 GGTTAGGGTATAACGTGTCT

151 SEQ TD N0:1200 -13.7 -23.2 69.2 -7.5 -2 -5.3 TTAATTACGTGTAGCTTCTT

435 SEQ ID N0:1201 -13.7 -20.2 62 -6.5 0 -5 TTTAATTACGTGTAGCTTCT

436 SEQ ID N0:1202 -13.7 -20.2 62 -6.5 0 -5 GTTCCTTTTTGGGCTTCTTG

573 SEQ ID N0:1203 -13.7 -25.6 75.8 -11.4 -0.2 -3.8 ATGGATCACGAAGAAACGTA

1020 SEQ ID N0:1204 -13.7 -18.9 56 -4.3 -0.8 -6 AACGCTGAGACATTGCCCAG

1096 SEQ ID N0:1205 -13.7 -25.7 69.9 -12 0 -4.l TGCAAACATTTATTCCTCAG

1288 SEQ ID N0:1206 -13.7 -20.4 61.1 -6.7 0 -4.7 ACATTAACAAATGTCCCATT

1375 SEQ ID N0:1207 -13.7 -19.9 58.6 -4.9 -1.2 -6 CAATAGTCTTGAGTCATGAG

1606 SEQ ID N0:1208 -13.7 -19.8 61.9 -6.1 0 -7.4 TCCCAATAGTCTTGAGTCAT

1609 5EQ ID N0:1209 -13.7 -23.6 69.8 -9.4 -0.2 -5.8 GATGCTTCTGAAGAACTTTC

1918 SEQ ID N0:1210 -13.7 -20.2 61.9 -6 0 -8.3 GATGAGGGGAGCAGAATTTT

2121 SEQ ID N0:1211 -13.7 -22.1 65.5 -8.4 0 -4.1 CTCCTGCTTTCGCCGTCTTC

2275 SEQ ID N0:1212 -13.7 -29.9 82.1 -16.2 0.2 -3.6 TGCCCTCTGGGGGAAGTTGT

2475 SEQ ID N0:1213 -13.7 -29.2 81.1 -13.3 -2.2 -8.7 CCTCCAGCATCTCCATTGAA

2544 SEQ ID N0:1214 -13.7 -26.9 74.4 -13.2 0 -4.1 GCCTTCATGGATTTCTATAA

3578 SEQ ID N0:1215 -13.7 -21.9 65.2 -7.6 -0.3 -4.4 TCAATTCCAGTATTATTGGA

3619 SEQ ID N0:1216 -13.7 -20.2 61.7 -5.5 -0.9 -6.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GCTGGGGTTGTTTATGAATG

3746 SEQ ID N0:1217 -13.7 -22.6 67.1 -8.9 0 -2.8 TACACCCTTCAGTAAAACAA

3972 SEQ ID NO:1218 -13.7 -19.9 58.5 -6.2 0 -3.1 AATACTGTAGCAGGTTTTTC

4058 SEQ ID NO:1219 -13.7 -20.7 64.4 -6.1 -0.7 -5.3 CAAGAAATCTAGCCAGCACC

4286 SEQ ID N0:1220 -13.7 -23.4 66 -9.7 0 -4.6 GTTTTTCCACCGAGCTTGCT

4637 SEQ TD N0:1221 -13.7 -27.8 77.4 -14.1 0 -5.9 AAGATGTCTTGACCTCCAAA

4903 SEQ ID N0:1222 -13.7 -21.9 63.6 -7.4 -0.6 -5.9 AAAGATGTCTTGACCTCCAA

4904 SEQ 2D N0:1223 -13.7 -21.9 63.6 -7.4 -0.6 -6.8 TTTTTTCTGTTCCTCTGTCA

4925 SEQ ID N0:1224 -13.7 -24 73.6 -10.3 0 -l.7 AAAGCAAAGAGCAGCGTGCG

5395 SEQ ID N0:1225 -13.7 -23.2 64.2 -7.3 -2.2 -8.5 TAGATAAACATGACCAGGAA

5458 SEQ ID N0:1226 -13.7 -18.1 55.2 -4.4 0 -5.2 CAGCCAGCAGAGGTTGTAAT

5584 SEQ ID N0:1227 -13.7 -25 72.3 -10.7 -0.3 -4.7 GGGTCGCATCGGGATCAAAC

5868 SEQ ID N0:1228 -13.7 -25.4 70 -11.7 0 -6.9 AGGGCAGCTGCAAAATCAGA

5911 SEQ ID NO:1229 -13.7 -23.7 67.7 -7.1 -0.7 -14 TTGTAATAGGCTCATAAGAG

6129 SEQ ID N0:1230 -13.7 -18.6 58.3 -3.9 -0.9 -4.4 TGGACCTCCTCTTGAAGTCC

6544 SEQ ID N0:1231 -13.7 -27.1 76.5 -11.4 -2 -5.7 CTGCTTCACAGAGTTGCAGT

6635 SEQ ID NO:1232 -13.7 -25.3 75.4 -9.9 -1.7 -6.6 GGAAGTTAAAAATAGAATGG

6799 SEQ ID N0:1233 -13.7 -14.1 47.4 0 0 -2.6 TTTTTCTAGAATGGATTTAT

7253 SEQ ID N0:1234 -13.7 -17 55.1 -2.8 0 -7.7 ATACTTTAGATATATTTTTC

7267 SEQ ID N0:1235 -13.7 -15.5 52.4 -1.8 0 -4.3 GATACATCCCTGTTGGAAGC

7497 SEQ ID NO:1236 -13.7 -24.7 70.5 -9.9 -1 -5.4 CATTCTTTAACCAAGACTTG

7607 SEQ ID N0:1237 -13.7 -19.3 58.6 -4.6 -0.9 -5.3 AACTTTGCCACTTTGTTCAT

8278 SEQ ID N0:1238 -13.7 -22.8 67.1 -9.1 0 -3.1 AGCTGCTTTTAATTTGCCTT

8653 SEQ ID N0:1239 -13.7 -23.7 69.2 -10 0 -6.7 AGGTGGTTACTACTATTATT

8732 SEQ ID N0:1240 -13.7 -20.1 63 -5.9 -0.1 -3.9 TCTTTCTCTGTGGATAAGAA

50 SEQ ID N0:1241 -13.6 -20 62.1 -5.7 -0.5 -3.8 TTTCTTTCTTTCTCTGTGGA

56 SEQ ID N0:1242 -13.6 -22.8 71.1 -9.2 0 -2.2 TAATTACGTGTAGCTTCTTG

434 SEQ ID N0:1243 -13.6 -20.1 61.6 -6.5 0 -4.7 TGTGCCATCTTTTCATCCTG

460 SEQ ID N0:1244 -13.6 -25.7 74.3 -12.1 0 -3 GGTTTGTTCTCATCATCATT

598 SEQ ID N0:1245 -13.6 -22.6 69.9 -9 0 -1.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTGGTTTGTTCTCATCATCA

600 SEQ ID N0:1246 -13.6 -22.6 69.7 -9 0 -1.9 CATTAACAAATGTCCCATTT

1374 5EQ ID N0:1247 -13.6 -19.8 58.5 -4.9 -l.2 -5.5 GTTACATTAACAAATGTCCC

1378 SEQ ID N0:1248 -13.6 -20.1 59.8 -4.9 -1.5 -6.6 GCTGTGTTCATCATCAGCAA

2249 SEQ ID N0:1249 -13.6 -24.3 72.2 -7.2 -3.5 -8.5 GCCGTCTTCAAATGTGCTGT

2264 SEQ ID N0:1250 -13.6 -26 73.3 -12.4 0 -3.6 ATGCTCACGGCTCTTTGCCT

2575 SEQ ID N0:1251 -13.6 -28.9 79.9 -12.6 -2.7 -8.3 CCATGCATCACAGCAGTCCC

2690 SEQ ID N0:1252 -13.6 -29.3 80.3 -14.4 -1.2 -7.5 AGTCTGAATGATCGCAGTAC

3016 SEQ ID N0:1253 -13.6 -21.8 65.3 -7.6 -0.3 -6.3 AGACGATGATGGCCAACACC

3132 SEQ ID N0:1254 -13.6 -24.9 67.8 -11.3 0 -6.3 GCCAGCGACCTCCATACAGT

3320 5EQ TD N0:1255 -13.6 -29.7 79.6 -16.1 0 -4.1 GGCCAGCGACCTCCATACAG

3321 SEQ ID N0:1256 -13.6 -29.7 78.7 -16.1 0 -7 CACCAGATCTTCCCTTTGCC

4030 SEQ ID N0:1257 -13.6 -28.9 78.9 -15.3 0 -5.8 CACAACCACCCTCATGCCTT

4427 SEQ ID N0:1258 -13.6 -28.8 75.7 -15.2 0 -4.4 TTTTTCTGTTCCTCTGTCAT

4924 SEQ ID N0:1259 -13.6 -23.9 73.2 -10.3 0 -1.8 AGGGAAACGAGCTTCAGCAC

5197. SEQ ID N0:1260 -13.6 -23.8 67.7 -9.3 -0.7 -6.6 TCAAAGCAAAGAGCAGCGTG

5397 SEQ ID N0:1261 -13.6 -21.7 62.4 -7.2 -0.8 -5.4 ATGGCATAGATAAACATGAC

5464 SEQ ID N0:1262 -13.6 -18 55.6 -4.4 0 -5.2 GCCAGCAGAGGTTGTAATTT

5582 SEQ ID N0:1263 -13.6 -24.5 71.6 -10.9 0 -5.7 TCCCAGCCAGCAGAGGTTGT

5587 SEQ ID N0:1264 -13.6 -30.4 84.6 -15.9 -0.8 -5.2 TCTTGTTTACGTTTCAAAGT

6151 SEQ ID N0:1265 -13.6 -19.8 61.1 -6.2 0 -4.8 CACTTCACTGTCTTGTATAG

6604 SEQ ID NO:1266 -13.6 -21.3 66 -7.7 0 -2.6 GTTCTTGTATACTGTTTCTT

6927 SEQ ID N0:1267 -13.6 -21.2 67.3 -7.6 0 -6.3 AGGAGACTTTACAGGCACAT

7115 SEQ ID N0:1268 -13.6 -22.9 67.8 -8.8 -0.2 -4.3 AGATATATTTTTCTAGAATG

7260 SEQ ID N0:1269 -13.6 -15.3 51.4 -1.1 0 -7.7 CTTTAGATATATTTTTCTAG

7264 SEQ ID N0:1270 -13.6 -16.2 54 -2.6 0 -4.3 ATAGGGTATTTAATTGACAT

7363 SEQ ID N0:1271 -13.6 -17.8 56.3 -4.2 0 -2.9 GCTATATATATATTTGCCCA

7420 SEQ ID N0:1272 -13.6 -21.9 64.4 -7.8 0 -7.9 ACTTGTGAAGAATGGATTGA

7592 SEQ ID N0:1273 -13.6 -18.7 57.6 -4.6 -0.2 -3.1 GACTTGTGAAGAATGGATTG

7593 SEQ ID N0:1274 -13.6 -18.7 57,6 -4.6 -0.2 -3.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGACTTGTGAAGAATGGATT

7594 SEQ ID N0:1275 -13.6 -18.7 57.8 -4.6 -0.2 -3.1 AGTAGGCAGTATCCAGTGTG

7771 SEQ ID N0:1276 -13.6 -25 75.5 -10.9 -0.2 -3.7 ACTGCTTGGTGAATCATGCA

8622 5EQ ID NO:1277 -13.6 -23.8 69.2 -8.3 -1.9 -6.4 TTTCCACCTTATATAATTTA

9078 SEQ ID NO:1278 -13.6 -18.8 57.9 -5.2 0 -5.3 TTCTTTCTTTCTCTGTGGAT

55 SEQ ID N0:1279 -13.5 -22.7 70.7 -9.2 0 -2.3 TGGTTTGTTCTCATCATCAT

599 SEQ ID N0:1280 -13.5 -22.5 69.3 -9 0 -1.8 TTGGCTTTGGTTTGTTCTCA

606 SEQ ID N0:1281 -13.5 -24.5 74.1 -11 0 -3.7 ATAAGTGACTCAAAGGTATA

964 SEQ ID N0:1282 -13.5 -17.4 55.3 -3.9 0 -3.3 ATCCAGCCAGTTCCATGGAT

1034 SEQ ID N0:1283 -13.5 -27.8 77.5 -12.6 -1.7 -10 CTGAGCCATTTCCACAGAGT

1476 SEQ ID N0:1284 -13.5 -26 74.2 -12.5 0 -3.5 ACAGATGTATCCTTCTGGAC

1508 SEQ ID N0:1285 -13.5 -23.1 69 -8 -1.6 -7.2 GTGGCCTGATTCTGCTCCTC

1753 SEQ ID NO:1286 -13.5 -29.5 84.1 -15.4 -0.3 -7.2 CCATTTGCTGGAAGCCCTGG

2377 SEQ ID N0:1287 -13.5 -28.6 76.5 -12.9 -2.2 -8.6 AATCCTCCAGCATCTCCATT

2547 SEQ ID N0:1288 -13.5 -26.7 74.8 -13.2 0 -4.l TTTTTGGAAACACTCCCGCA

3536 SEQ ID NO:1289 -13.5 -24.5 67.2 -10.2 -0.6 -4.8 CTTCATGGATTTCTATAACT

3576 SEQ ID N0:1290 -13.5 -19.2 59.7 -5.7 0 -5 CCCATCTCTAAGATAATTAA

3653 SEQ ID N0:1291 -13.5 -19 57.4 -4.8 -0.5 -4.9 GGTTCCATTCCCATCTCTAA

3662 SEQ ID N0:1292 -13.5 -26.4 75 -12.9 0 -3.5 TTTTGATAGTCTTTCGCTGT

4164 SEQ ID N0:1293 -13.5 -22.4 68.1 -8.9 0 -3.4 GTTTTGATAGTCTTTCGCTG

4165 SEQ ID N0:1294 -13.5 -22.4 68.1 -8.9 0 -3.1 TGGTTTTGATAGTCTTTCGC

4167 SEQ ID N0:1295 -13.5 -22.7 68.8 -9.2 0 -2.9 AAAGTTTACTTTCACGTTTT

4652 SEQ ID N0:1296 -13.5 -18.6 58 -3.9 -1.1 -8.6 GATTTTCTTCATATACAGGC

4776 SEQ ID N0:1297 -13.5 -20.7 64.1 -7.2 0 -2.8 TTGACCTCCAAACTTCTTTT

4895 SEQ ID N0:1298 -13.5 -22.3 65 -8.8 0 -2.6 TAGGGTCATGTATTTGCCCT

5120 SEQ ID N0:1299 -13.5 -26.2 75.5 -10.5 -2.2 -8.5 ACGTAGGATTCGGCCAATCC

5351 SEQ ID N0:1300 -13.5 -26.4 71.7 -10.5 -1.7 -12.8 GCAAAGAGCAGCGTGCGGAT

5392 SEQ ID N0:1301 -13.5 -26.4 71.5 -10.7 -2.2 -8.5 ~

CATCCCAAAGATGGCATAGA

5474 5EQ ID N0:1302 -13.5 -23 65 -8 -1.4 -6.9 AGCCAGCAGAGGTTGTAATT

5583 SEQ ID N0:1303 -13.5 -24.4 71.5 -10.9 0 -4.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Tntra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TAACTGAGCTGCCAGGGTGA

5664 SEQ ID N0:1304 -13.5 -26.1 73.8 -11.7 -0.6 -9.3 CACGGTCTCCCTTAACTGAG

5676 SEQ ID N0:1305 -13.5 -25.5 71.2 -11.4 -0.3 -3.8 TCGGGATCAAACTTTTCCCA

5860 SEQ ID N0:1306 -13.5 -24.4 67.8 -8.3 -2.6 -7.9 TCCTCTTGTTTACGTTTCAA

6154 SEQ ID N0:1307 -13.5 -22.6 67.2 -9.l 0 -4.8 GTGTAGTTACAATGTTCACT

6752 SEQ ID NO:1308 -13.5 -20.8 64.7 -6.8 -0.2 -5.4 CAGATATGGAAGTTAAAAAT

6806 SEQ ID N0:1309 -13.5 -14.3 47.8 0 -0.6 -4.9 AGAGACAGGGTTCTTGTATA

6936 SEQ ID N0:1310 -13.5 -21.8 67.4 -7.6 -0.4 -4.5 GACTATCAGCTTTGCTGAGA

7342 SEQ ID N0:1311 -13.5 -23.3 69.7 -6.7 -3.1 -10.3 CTTTAACCAAGACTTGTGAA

7603 SEQ ID N0:1312 -13.5 -19.2 58 -5.7 0 -4.9 CCATTATCTAACTTTTATTT

8016 SEQ ID N0:1313 -13.5 -18.1 56.9 -4.6 0 -1.6 ATAAAAGGTTATATTGAGGC

8136 SEQ ID N0:1314 -13.5 -17.2 54.6 -3.7 0 -2.8 TTTGTAATAGCTTTAGTGCA

8230 SEQ ID NO:1315 -13.5 -20.4 63.2 -6 -0.8 -5.2 CCCCAGAGCCCAATAAGTGA

8487 SEQ ID N0:1316 -13.5 -27.9 73.6 -14.4 0 -3.3 AATCATGCACTAGTTTGTTG

8611 SEQ ID N0:1317 -13.5 -20.4 62.7 -6.9 0 -5.7 TTGGTGAATCATGCACTAGT

8617 SEQ ID N0:1318 -13.5 -22 66 -7.3 -1.1 -6.2 TCCACCTTATATAATTTATT

9076 SEQ ID NO:1319 -13.5 -18.7 57.6 -5.2 0 -5.3 TTCCACCTTATATAATTTAT

9077 SEQ ID N0:1320 -13.5 -18.7 57.6 -5.2 0 -5.3 TATCTCATCCCTGTCAAACC

264 SEQ ID No:1321 -13.4 -24.5 69.7 -11.1 0 -2 TACGTGTAGCTTCTTGCATA

430 SEQ ID N0:1322 -13.4 -23.2 69 -8.2 -1.5 -7.6 CTGCAGCACGTTTTTCGATA

543 SEQ ID N0:1323 -13.4 -24 68.4 -9.6 -0.9 -8.7 GGATCCAGGTCCTCCAGGGG

697 SEQ ID N0:1324 -13.4 -31.3 86.2 -16.5 -1.2 -9.7 TTCTTATTGATATAGTAGGG

715 SEQ ID N0:1325 -13.4 -18.6 59.5 -5.2 0 -2.7 TTGTTTTCAGTGCTCGGAGA

1131 SEQ ID NO:1326 -13.4 -24.2 72 -10.3 -0.1 -6.4 AATCGCTTGGGGGCCACTGC

1305 SEQ ID N0:1327 -13.4 -29.5 79 -15.2 -0.8 -6.8 GCAGCACGTAATGTCAACTG

1639 SEQ ID N0:1328 -13.4 -22.9 65.9 -9.5 0 -4.9 TGTTGCTTTTTAAGCTGTTC

1819 SEQ ID N0:1329 -13.4 -21.8 67.2 -5.8 -2.6 -7.1 TTCTTCCTGTTGCTTTTTAA

1826 SEQ ID NO:1330 -13.4 -22.2 67.2 -8.8 0 -3.6 TGCCTGAGCTTCTTCCTGTT

1835 SEQ ID N0:1331 -13.4 -28.1 80.7 -13.5 -1.1 -5.2 ACTCAACTTTGATGCTTCTG

1928 SEQ ID N0:1332 -13.4 -21.5 64.8 -8.1 0 -5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGTCTGTGCGGCACAAACAG

2302 SEQ ID N0:1333 -13.4 -24.4 68.8 -8.6 -1.1 -12.9 CTAACGTTACTGTTGCGTCG

2329 SEQ ID N0:1334 -13.4 -23.3 65.9 -8.9 -0.9 -7.8 TGTGCATCTTCCCATTTGCT

2388 SEQ ID N0:1335 -13.4 -27.1 77 -12.4 -1.2 -5.4 GAGTGGAAGAAGTCGTTCAT

3247 SEQ ID N0:1336 -13.4 -21.6 64.8 -6.9 -l.2 -4.1 AACTTTTGGCTTTCTAAAAA

3560 SEQ ID N0:1337 -13.4 -17 53.5 -2.8 -0.6 -4.3 GGTTTAAAGTCTTCTTCGGG

3946 SEQ ID N0:1338 -13.4 -22.5 67.2 -8.4 -0.5 -4.7 AATGCACCACTACTGAGAAG

4120 5EQ TD N0:1339 -13.4 -21.1 61.8 -7 -0.4 -6.3 TCAGCATATTCTAGCATGGT

4183 SEQ ID N0:1340 -13.4 -23.3 70.2 -8.9 -0.9 -5 GTCAGCATATTCTAGCATGG

4184 SEQ ID N0:1341 -13.4 -23.3 70.2 -8.9 -0.9 -5 CTGAAGTTTAACATCTCGTG

4757 SEQ ID N0:1342 -13.4 -19.8 60.2 -6.4 0 -3.5 GAGGGAAACGAGCTTCAGCA

5192 SEQ ID N0:1343 -13.4 -24.2 68.4 -9.9 -0.7 -6.6 AGATGGCATAGATAAACATG

5466 SEQ ID N0:1344 -13.4 -17.8 55.3 -4.4 0 -4.7 CAAAGTGGTTGTAATAGGCT

6137 SEQ ID N0:1345 -13.4 -20.8 62.6 -7.4 0 -3.7 ACACCTCCTCTTGTTTACGT

6159 SEQ ID N0:1346 -13.4 -26 74 -12.6 0 -4.6 TTGACACATATACTTTACCT

6522 SEQ ID N0:1347 -13.4 -20 60.6 -6.6 0 -2.4 CCTATAGTCTAGGTAGCCAT

6719 SEQ ID N0:1348 -13.4 -25.1 73.8 -10.8 -0.8 -5.4 ATCCCTATAGTCTAGGTAGC

6722 5EQ ID NO:1349 -13.4 -24.8 74.4 -10 -1.3 -5.2 ATCCTTTAAATATTAGAGGA

7131 SEQ ID N0:1350 -13.4 -17.7 55.7 -2.2 -2.1 -8 CATAGGCAATATTAACATGC

7558 SEQ ID N0:1351 -13.4 -18.9 57.5 -5 -0.1 -5 CATAATGGACAGAGCAGGTT

7653 SEQ ID N0:1352 -13.4 -22 65.2 -8.6 0 -4.1 CATAGTTGGTAGAAAATGAT

8113 SEQ ID NO:1353 -13.4 -16.9 53.8 -3.5 0 -2.5 AGAGCCCAATAAGTGAAATG

8483 SEQ ID N0:1354 -13.4 -19.8 58.3 -6.4 0 -3.3 CCTTTGTTCTGTAGTACTGC

8637 SEQ ID N0:1355 -13.4 -24.4 73.7 -11 0 -6.8 GTTAGGGTATAACGTGTCTT

150 SEQ ID N0:1356 -13.3 -22.1 66.9 -7.5 -1.2 -5.3 TTCTCTAGTAAAAAGGCGGA

512 SEQ ID N0:1357 -13.3 -20.1 60 -6.8 0 -4 TCAGGAGGGTTGCTCAAGGT

907 SEQ ID N0:1358 -13.3 -26.1 76.9 -10.8 -2 -5.7 AAGCAAAACCCTCTTGCCAA

991 SEQ ID N0:1359 -13.3 -24 65.2 -8.7 -2 -6.2 ATTGTTTTCAGTGCTCGGAG

1132 SEQ ID N0:1360 -13.3 -23.6 70.5 -10.3 0 -5.9 ATCCTTCTGGACACTGGCCT

1500 SEQ ID N0:1361 -13.3 -28.4 79.2 -13.7 -1.3 -9.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTCTTGAGTCATGAGTCGAA

1601 SEQ ID N0:1362 -13.3 -22.4 67.7 -7.6 0 -10.9 TTTTTAAGCTGTTCGAGCAT

1813 SEQ ID N0:1363 -13.3 -21.7 64.8 -6.1 -2.3 -7.3 CTTCTTCCTGTTGCTTTTTA

1827 SEQ ID N0:1364 -13.3 -23.8 71.7 -10.5 0 -3.6 CTGATGCTGCCGCAACTGCC

1851 SEQ ID N0:1365 -13.3 -29.5 77.4 -14.6 -1.6 -8 TGATGCTTCTGAAGAACTTT

1919 SEQ ID N0:1366 -13.3 -19.8 60.4 -6 0 -7.6 TTGATGCTTCTGAAGAACTT

1920 SEQ ID N0:1367 -13.3 -19.8 60.4 -6 0 -8.3 TTTGATGCTTCTGAAGAACT

1921 SEQ ID N0:1368 -13.3 -19.8 60.4 -6 0 -8.3 TGAGGGGAGCAGAATTTTTT

2119 SEQ ID N0:1369 -13.3 -21.7 64.9 -8.4 0 -4.1 GCTCAGTCATGGGGTAGTGC

2811 SEQ ID N0:1370 -13.3 -27.6 82.4 -14.3 0 -4.7 ATCCCAGTAAAGACCAGGTT

2857 SEQ ID N0:1371 -13.3 -24.6 69.6 -11.3 0 -5.9 GAACACAATCAGGAAGGAGT

3263 SEQ ID N0:1372 -13.3 -20.2 60.5 -6.9 0 -2.9 GGAACACAATCAGGAAGGAG

3264 SEQ ID N0:1373 -13.3 -20.2 60 -6.9 0 -2.9 CGTTTTTCCACCGAGCTTGC

4638 SEQ ID N0:1374 -13.3 -27.7 75.2 -14.4 0 -5.5 CACGTTTTTCCACCGAGCTT

4640 SEQ ID NO:1375 -13.3 -26.8 72.9 -13.5 0 -5.2 ATGAGGATCATGATGCTGAT

5059 SEQ TD NO:1376 -13.3 -21.4 64.1 -6.5 -1.5 -8.5 GAAGAGCAGGAGGCCGATGT

5441 SEQ ID NO:1377 -13.3 -26.4 73.4 -12.5 -0.3 -7.7 CCCAACAGATGGGTCCCCAC

5693 SEQ ID N0:1378 -13.3 -30.4 78.9 -14.5 -2.6 -8.8 TTCGAAGGGCATCCATCTCT

6066 SEQ ID N0:1379 -13.3 -25.8 73 -11.3 -1.1 -7.9 CACCTCCTCTTGTTTACGTT

6158 SEQ ID N0:1380 -13.3 -25.9 73.8 -12.6 0 -4.8 GTTTTATAGGTAAGTCAATC

6276 SEQ ID N0:1381 -13.3 -18.3 59 -5 0 -2.3 TAGATATATTTTTCTAGAAT

7261 SEQ ID N0:1382 -13.3 -15 50.8 -1.1 0 -7.7 GCATAGGCAATATTAACATG

7559 SEQ ID N0:1383 -13.3 -18.9 57.5 -4.7 -0.8 -6.3 GACAGAGCAGGTTGAATTCA

7646 SEQ ID N0:1384 -13.3 -22.2 66.4 -8.4 0 -8.1 TTGACATAATGGACAGAGCA

7657 SEQ ID N0:1385 -13.3 -20.4 61.1 -7.1 0 -4.1 GAGGCAACCATAGTTGGTAG

8121 SEQ TD N0:1386 -13.3 -23.6 69.1 -7.9 -2.4 -8.7 GCTCACCATTCTTTTTAGCA

8420 SEQ ID N0:1387 -13.3 -24.7 72.6 -10.7 -0.4 -4.1 AAAGTTGGAACATGTGAACT

g7p2 SEQ ID N0:1388 -13.3 -18.1 55.7 -4.1 -0.3 -8.3 GGTTACTACTATTATTATCA

g72g SEQ ID N0:1389 -13.3 -18.5 59 -5.2 0 -2.3 ATTGAAAGAAGATGAGTTTA

8796 SEQ ID N0:1390 -13.3 -15.5 51 -2.2 0 -2.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCTGCTTATGCCTCCGCTTC

4 SEQ ID N0:1391 -13.2 -28.9 80.3 -14.8 -0.8 -5 TAGGGTATAACGTGTCTTCC

148 SEQ ID NO:1392 -13.2 -23.2 68.6 -10 0 -5.3 GAATTATCTCATTTATTCTT

352 SEQ ID N0:1393 -13.2 -17.5 56.6 -4.3 0 -4.1 ACATTTAATTACGTGTAGCT

439 SEQ ID N0:1394 -13.2 -19.7 60.1 -6.5 0 -5 TTTCGACCAGCCTTCACACA

1525 SEQ ID N0:1395 -13.2 -26.6 73.3 -13.4 0 -4.6 CAGCAGCACGTAATGTCAAC

1641 SEQ ID N0:1396 -13.2 -22.7 65.5 -9.5 0 -5.4 GATGCTGCCGCAACTGCCTG

1849 SEQ ID N0:1397 -13.2 -29.5 77.4 -14.7 -1.6 -8 AACTCAACTTTGATGCTTCT

1929 SEQ ID N0:1398 -13.2 -20.8 62.8 -7.6 0 -5 ATGAGGGGAGCAGAATTTTT

2120 SEQ ID N0:1399 -13.2 -21.6 64.6 -8.4 0 -4.1 TCTGTGCGGCACAAACAGTG

2300 SEQ ID N0:1400 -13.2 -24.4 68.8 -8.6 -1.4 -13.2 TGTTGCGTCGCTCTCCATGT

2319 SEQ ID N0:1401 -13.2 -28.6 79.8 -14.8 -0.3 -6.9 GTGCTGTGCATCTTCCCATT

2392 SEQ ID NO:1402 -13.2 -28.2 80.2 -13.9 -1 -5.5 TATGCTCACGGCTCTTTGCC

2576 SEQ ID N0:1403 -13.2 -27.7 77.4 -12.6 -1.9 -7.4 GGCCATAAAGAGGGTATTTA

2786 SEQ ID N0:1404 -13.2 -21.8 64.2 -8.6 0 -7 TGGCCATAAAGAGGGTATTT

2787 SEQ ID N0:1405 -13.2 -22.1 64.6 -8.4 0 -8.3 GCAGTACAGACAATCCCTCC

3003 SEQ ID N0:1406 -13.2 -26.8 74.9 -12.9 -0.4 -5.3 CTGAATGATCGCAGTACAGA

3013 SEQ ID N0:1407 -13.2 -21.5 63.2 -7.6 -0.4 -5.7 AACACCAAGGTGAGGTTTCC

3118 SEQ ID NO:1408 -13.2 -24.4 69.7 -10.1 -0.8 -9.6 GAAGGAGTGGAAGAAGTCGT

3251 SEQ ID N0:1409 -13.2 -21.5 63.8 -7.8 -0.2 -3 AATTCCAGTATTATTGGACA

3617 SEQ ID NO:1410 -13.2 -20 60.8 -5.3 -1.4 -7.2 TTTTCTGTTCCTCTGTCATA

4923 SEQ ID N0:1411 -13.2 -23.5 72.1 -10.3 0 -1.8 GTATGGGTTTCTGAGGTTTC

4977 SEQ TD N0:1412 -13.2 -23.6 73 -10.4 0 -2.8 GCGAGGTATGGGTTTCTGAG

4982 SEQ ID N0:1413 -13.2 -25 73.1 -11.8 0 -3.4 GGAATTTGTTTGCTGGGCGA

4998 5EQ ID N0:1414 -13.2 -25 70.6 -11 -0.6 -4.9 TGTCTGAGGGAAACGAGCTT

5197 SEQ ID N0:1415 -13.2 -23.1 66.6 -9.9 0 -5.7 TTTTACTTTTGATTTTCTCT

6457 SEQ ID N0:1416 -13.2 -18.9 60.6 -5.7 0 -2.2 AATCCTTTAAATATTAGAGG

7132 5EQ ID N0:1417 -13.2 -16.4 52.7 -2.2 -0.9 -5.6 TGACATAATGGACAGAGCAG

7656 SEQ ID N0:1418 -13.2 -20.3 60.9 -7.1 0 -4.1 CATTTAATTACGTGTAGCTT

438 SEQ ID NO:1419 -13.1 -19.6 59.9 -6.5 0 -5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCCTGCACATTTAATTACGT

445 SEQ ID N0:1420 -13.1 -21.9 63.7 -8.8 0 -4.8 CATCCTGCACATTTAATTAC

447 SEQ ID N0:1421 -13.1 -20.6 61.5 -7.5 0 -4.6 TTTGGCTTTGGTTTGTTCTC

607 SEQ ID N0:1422 -13.1 -23.9 73.3 -10.8 0 -3.7 TCAAAAGCAGAATCGCTTGG

1315 SEQ ID N0:1423 -13.1 -20.6 60 -5.7 -1.8 -9.3 TTACATTAACAAATGTCCCA

1377 SEQ ID N0:1424 -13.1 -19.6 58.1 -4.9 -1.5 -6.6 AATATCATGTATGTTTTCCC

1660 SEQ ID N0:1425 -13.1 -20.9 63.2 -7.8 0 -4.5 TGCTTCTGAAGAACTTTCCA

1916 SEQ ID NO:1426 -13.1 -22.3 65.6 -8.5 -0.4 -8.3 GACTAACGTTACTGTTGCGT

2331 SEQ ID N0:1427 -13.1 -22.9 66.1 -9.3 -0.2 -7.8 AGTGCTGTGCATCTTCCCAT

2393 SEQ ID NO:1428 -13.1 -28.1 80.2 -13.9 -1 -5.5 ACACTCCCGCATCTTATTTT

3527 SEQ ID NO:1429 -13.1 -25.1 70.7 -12 0 -3.6 GGGTTGTTTATGAATGACAT

3742 SEQ ID N0:1430 -13.1 -20.2 61.5 -6.3 -0.6 -5.4 CAGCATATTCTAGCATGGTT

4182 SEQ ID N0:1431 -13.1 -23 68.9 -8.9 -0.9 -5 TCACGTTTTTCCACCGAGCT

4641 SEQ ID N0:1432 -13.1 -27.1 74.1 -14 0 -5 CTTTCACGTTTTTCCACCGA

4644 SEQ ID N0:1433 -13.1 -25.5 70.5 -12.4 0 -4.5 CGCCAACATTATCAAAGTTT

4665 SEQ ID N0:1434 -13.1 -20.4 59.5 -7.3 0 -3.7 TGAAGTTTAACATCTCGTGA

4756 SEQ ID N0:1435 -13.1 -19.5 59.6 -6.4 0 -3.6 GTAGTGTCTGAGGGAAACGA

5201 SEQ ID N0:1436 -13.1 -22.4 66 -9.3 3 -3.5 TTTGATCAGACGTAGGATTC

5360 SEQ ID N0:1437 -13.1 -20.7 63.2 -7.6 0 -6.6 AACTGAGCTGCCAGGGTGAA

5663 SEQ ID N0:1438 -13.1 -25.7 72 -11.7 -0.6 -9.3 CCATGGGCAGATCCATGGCA

5976 SEQ ID N0:1439 -13.1 -29 78.4 -11.5 -4.4 -11.4 ATAGGCTCATAAGAGACTTT

6124 SEQ ID N0:1440 -13.1 -20.2 62.2 -5.9 -1.1 -4.5 ATGGACCTCCTCTTGAAGTC

6545 SEQ ID N0:1441 -13.1 -25.1 72.9 -11.4 -0.3 -5.l TAGGTAGCCATTGGGTTTCT

6710 SEQ ID N0:1442 -13.1 -25.5 75.4 -11.8 -0.3 -6.3 GAAGTTAAAAATAGAATGGA

6798 SEQ ID N0:1443 -13.1 -13.5 46.2 0 0 -2.8 AGAGGAGACTTTACAGGCAC

7117 SEQ ID N0:1444 -13.1 -22.8 68.3 -8.8 -0.7 -5.2 TCTGCTTGACATAATGGACA

7662 SEQ ID N0:1445 -13.1 -21.3 63.3 -8.2 0 -3.6 GCAACCATAGTTGGTAGAAA

8118 SEQ ID N0:1446 -13.1 -21 62 -5.5 -2.4 -8.6 TTTATATCATGTTAGGTAAT

8303 SEQ ID N0:1447 -13.1 -17.1 55.6 -4 0 -4.7 TGGTTACTACTATTATTATC

g72g SEQ ID N0:1448 -13.1 -17.8 57.6 -4.7 0 -2.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGTATACATAGGAGTTTATA

8886 SEQ ID N0:1449 -13.1 -17.8 57.4 -4.7 0 -7.4 AGCTTCTTGCATACGAATTA

423 SEQ ID N0:1450 -13 -21.1 62.7 -6.5 -1.5 -5.3 TTTGGTTTGTTCTCATCATC

601 5EQ ID N0:1451 -13 -22 68.9 -9 0 -1.9 CAACGCTGAGACATTGCCCA

1097 SEQ ID N0:1452 -13 -26.4 70.7 -13.4 0 -3.3 CCACTGCAAACATTTATTCC

1292 SEQ ID NO:1453 -13 -22.2 63.6 -9.2 0 -4.9 TACATTAACAAATGTCCCAT

1376 SEQ ID N0:1454 -13 -19.5 57.8 -4.9 -l.5 -6.6 TCCACAGAGTAAAGGGTCTT

1466 SEQ ID N0:1455 -13 -23.1 68.4 -10.1 0 -3.6 TTCCACAGAGTAAAGGGTCT

1467 SEQ ID N0:1456 -13 -23.1 68.4 -10.1 0 -3.6 GGCCTGATTCTGCTCCTCAT

1751 SEQ ID N0:1457 -13 -29 81.6 -15.4 -0.3 -6.4 TGGCCTGATTCTGCTCCTCA

1752 SEQ ID N0:1458 -13 -29 81.4 -15.4 -0.3 -7.2 CGCTGTCTTCAGATTCGGAT

2046 SEQ ID N0:1459 -13 -24.9 71.2 -10.4 -1.4 -5.7 TGATGAGGGGAGCAGAATTT

2122 SEQ ID N0:1460 -13 -22 65.1 -9 0 -4.1 CTGTGTTCATCATCAGCAAA

2248 SEQ ID N0:1461 -13 -21.8 65.4 -8.1 -0.4 -4.1 CCTGACTAACGTTACTGTTG

2334 SEQ ID N0:1462 -13 -22 64.1 -8.5 -0.2 -7.8 GGCCTGACTAACGTTACTGT

2336 SEQ ID N0:1463 -13 -24.9 70.4 -11.4 0 -7.9 TCTGAATGATCGCAGTACAG

3014 SEQ ID N0:1464 -13 -21.3 63.4 -7.6 -0.4 -6.3 GGCCAACACCAAGGTGAGGT

3122 SEQ ID N0:1465 -13 -27.5 75.1 -12.9 -1.3 -10.8 TGGCCAGCGACCTCCATACA

3322 SEQ ID N0:1466 -l3 -29.7 78.2 -16.1 -0.1 -8.3 TCTGAGCTAAATGAACTCAA

3421 SEQ ID N0:1467 -13 -18.3 56.4 -4 -1.2 -5.1 GTAGCAGCAAGGTTGTCTGA

3436 SEQ ID N0:1468 -l3 -24.8 74.2 -10.4 -1.3 -8.2 GACCTCCAAACTTCTTTTTC

4893 SEQ ID N0:1469 -13 -22.7 66.5 -9.7 0 -1.8 ATTTTTTCTGTTCCTCTGTC

4926 SEQ ID N0:1470 -13 -23.3 72.3 -10.3 0 -0.6 TGCTGGGCGAGGTATGGGTT

4988 SEQ ID N0:1471 -13 -28.1 78.8 -14.3 -0.6 -4.3 TTGCTGGGCGAGGTATGGGT

4989 SEQ ID N0:1472 -13 -28.1 78.8 -14.3 -0.6 -4.4 ACAGAATACTTTTCTATCAT

5296 SEQ ID N0:1473 -13 -18 57.2 -4 -0.9 -4.4 ATCAAAGCAAAGAGCAGCGT

5398 SEQ ID N0:1474 -13 -21.7 62.5 -7.1 -1.6 -5.7 ACTGAGCTGCCAGGGTGAAT

5662 SEQ ID N0:1475 -13 -26.4 74.4 -12.5 -0.6 -9.3 TTAACTGAGCTGCCAGGGTG

5665 SEQ TD N0:1476 -13 -25.6 72.9 -11.7 -0.6 -9.3 AAGAGGAGGATCCAGGGCAG

5924 SEQ ID N0:1477 -13 -25.5 72.9 -9.9 -2.6 -9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTCCCATTTAGTTTGTCAAT

6307 SEQ ID N0:1478 -13 -22.1 66 -9.1 0 -2.9 TTTGGCATGGACCTCCTCTT

6551 SEQ ID N0:1479 -13 -27.5 77.4 -14 -0.1 -5.1 GATAGCCTTTAAACTATGTT

7532 5EQ ID NO:1480 -13 -19.6 59.8 -5.9 -0.4 -5.8 ATTTGTAATAGCTTTAGTGC

8231 SEQ ID N0:1481 -13 -19.7 61.9 -6.7 0 -4.6 TTCTTTTTAGCAATAGCAAT

8412 SEQ ID N0:1482 -13 -18.8 58.6 -4.1 -1.7 -4.3 TGCACTAGTTTGTTGTAAAA

8606 SEQ ID N0:1483 -13 -18.8 58.4 -5.3 -0.2 -6.1 GATGAGTTTAGATGCTTATA

8786 SEQ ID N0:1484 -13 -19.1 60.3 -6.1 0 -3.6 TTATATTGAAAGAAGATGAG

8800 SEQ ID N0:1485 -13 -13.9 47.5 -0.7 0 -2.2 GTGAP.TTATCTCATTTATTC

354 SEQ ID N0:1486 -12.9 -17.7 57.3 -4.3 -O.l -4 TTTCATCCTGCACATTTAAT

450 SEQ TD N0:1487 -12.9 -21.2 63.2 -8.3 0 -4.8 GTCAGGAGGGTTGCTCAAGG

908 SEQ ID N0:1488 -12.9 -26.1 76.9 -11.9 -1.2 -5 AGACATTGCCCAGGTCCACA

1089 SEQ ID N0:1489 -12.9 -28.5 78.1 -14.9 -0.5 -4.4 TTGGTTTCAAAAGCAGAATC

1321 SEQ ID N0:1490 -12.9 -18.5 57.3 -5.1 -0.1 -1.1 TCTGAGCCATTTCCACAGAG

1477 SEQ ID N0:1491 -12.9 -25.2 72.5 -11.4 -0.8 -4.6 GCTGTGCATCTTCCCATTTG

2390 SEQ ID N0:1492 -12.9 -27.1 77 -14.2 0 -5.4 TGCATCACAGCAGTCCCAGA

2687 SEQ TD N0:1493 -12.9 -27.9 78.5 -14.1 -0.8 -4.8 ACACAATCAGGAAGGAGTGG

3261 SEQ ID N0:1494 -12.9 -21.5 63.7 -7.8 -0.6 -4.8 GACGGTGAGGCTGGGGTTGT

3755 SEQ ID N0:1495 -12.9 -28.6 80.6 -15.7 0 -3.7 GTGACGGTGAGGCTGGGGTT

3757 SEQ TD N0:1496 -12.9 -28.6 80.6 -15.7 0 -3.7 ATACTGTAGCAGGTTTTTCG

4057 SEQ ID N0:1497 -12.9 -22.2 67 -8.4 -0.7 -5.3 GGTTTTGATAGTCTTTCGCT

4166 SEQ ID N0:1498 -12.9 -23.6 71 -10.7 0 -3.4 TCAAACATGTTACCCGTTGT

4573 SEQ ID N0:1499 -12.9 -23.1 65.6 -9.5 0 -9 ATCCCAAAGATGGCATAGAT

5473 SEQ ID N0:1500 -12.9 -22.3 63.9 -8 -1.3 -5 GACATCCCAAAGATGGCATA

5476 SEQ ID NO:1501 -12.9 -23.2 65.4 -8 -2.3 -8 TAGGCAAAGTTGGACATCCC

5488 SEQ ID N0:1502 -12.9 -24.4 69.1 -11.5 0 -4.9 GCAGATCATGCTGTTGCCAA

5555 SEQ ID N0:1503 -12.9 -26 73.6 -11.5 -1.6 -8.3 TCCCAACAGATGGGTCCCCA

5694 SEQ ID N0:1504 -12.9 -30.6 80 -14.5 -3.2 -8.6 TCTTCCATCTGTATTCGAAG

6079 SEQ ID N0:1505 -12.9 -21.9 65.3 -8.5 -0.2 -7.1 ATATGGAAGTTAAAAATAGA

6803 SEQ ID N0:1506 -12.9 -13.3 46 0 0 -2.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GATATGGAAGTTAAAAATAG

6804 SEQ ID N0:1507 -12.9 -13.3 46 0 0 -2.8 AGATATGGAAGTTAAAAATA

6805 SEQ ID N0:1508 -12.9 -13.3 46 0 0 -2.8 TTGAGAGACAGGGTTCTTGT

6939 SEQ ID NO:1509 -12.9 -23.1 70.4 -9.7 -0.2 -5 CTTTGCTGAGAGCAGAAGAT

7333 SEQ ID N0:1510 -12.9 -22 65.7 -6.7 -2.4 -8.2 ATAGACTATCAGCTTTGCTG

7345 SEQ ID N0:1511 -12.9 -21.8 66.3 -6.7 -2.2 -8.1 CATAGACTATCAGCTTTGCT

7346 SEQ ID N0:1512 -12.9 -22.5 67.7 -8.9 -0.4 -4.5 TGATAGCCTTTAAACTATGT

7533 SEQ ID N0:1513 -12.9 -19.5 59.4 -5.9 -0.4 -5.8 GTGATAGCCTTTAAACTATG

7534 SEQ ID N0:1514 -12.9 -19.5 59.4 -5.9 -0.4 -5.8 GTTGAATTCATTCTATCACC

7636 SEQ ID N0:1515 -12.9 -20.8 63.4 -7.2 -0.4 -8.4 TTCAAATTATTCTGCTTGAC

7672 SEQ ID N0:1516 -12.9 -18.6 58.1 -5.2 -0.2 -3.6 TGAAGAATAGGTTTTGTCAG

7789 SEQ ID N0:1517 -12.9 -18.5 58.4 -5.6 0 -2.7 TTATATTTGTAATAGCTTTA

8235 SEQ ID N0:1518 -12.9 -16.2 53.5 -3.3 0 -4.6 ACTTTGTTCATGGCTAAAAA

8269 SEQ ID N0:1519 -12.9 -18.5 56.8 -5.6 0 -4.7 CCCAGAGCCCAATAAGTGAA

8486 SEQ ID N0:1520 -12.9 -25.2 68.2 -12.3 0 -3.3 CTGCTTTTAATTTGCCTTTG

8651 SEQ ID NO:1521 -12.9 -22 65 -9.1 0 -3.6 TTTTTTTTTTTTTTTTTTTC

9094 SEQ ID N0:1522 -12.9 -16.2 54.7 -3.3 0 0 CTTTCTTTCTCTGTGGATAA

53 SEQ ID N0:1523 -12.8 -21.2 65.5 -8.4 0 -2.3 ATTTAATTACGTGTAGCTTC

437 SEQ ID N0:1524 -12.8 -19.3 60 -6.5 0 -5 GTGCCTGTGCCATCTTTTCA

465 SEQ ID NO:1525 -12.8 -28.3 80.7 -14.7 -0.6 -3 TCATCATTATCTTGTTCCTT

586 SEQ ID N0:1526 -12.8 -22.1 67.7 -9.3 0 -1.9 GGTTCTTTCCAGCTTCCAAG

633 SEQ ID N0:1527 -12.8 -26.3 76.1 -13.5 0 -4.5 ACCCTCTTGCCAAGATTTTT

984 SEQ ID N0:1528 -12.8 -25.3 71.1 -11.7 -0.6 -6.3 AATGTCCCATTTGAATCCAT

1366 SEQ ID NO:1529 -12.8 -22.8 64.9 -9.5 -0.2 -3.6 CATAGGCCATGGCCACCACA

1734 SEQ ID N0:1530 -12.8 -29.6 78.2 -12.2 -2.5 -17.4 GTGCTCTCTCTGTCTTCTTT

1985 SEQ ID N0:1531 -12.8 -25.9 80.7 -13.1 0 -3.6 TGTGTTCATCATCAGCAAAG

2247 SEQ ID N0:1532 -12.8 -20.9 63.7 -8.1 0 -4.1 TGCATCTTCCCATTTGCTGG

2386 SEQ ID NO:1533 -12.8 -27.1 76.1 -13 -1.2 -5.9 CTATGCTCACGGCTCTTTGC

2577 SEQ ID N0:1534 -12.8 -26.6 75.7 -12.6 -1.1 -4.9 AGACCAAGCTCCATTAAACT

2974 SEQ ID N0:1535 -12.8 -22.1 63.4 -9.3 0 -5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CCTCCACATTTGACAGACCA

2988 SEQ ID N0:1536 -12.8 -26.1 71.9 -13.3 0 -2.4 TGATCGCAGTACAGACAATC

3008 SEQ ID N0:1537 -12.8 -21.3 63.3 -7.8 -0.4 -5.7 CACTCCCGCATCTTATTTTT

3526 SEQ ID N0:1538 -12.8 -25 70.5 -12.2 0 -3.6 AAGCTCTTTGCTTATTTCAA

3635 SEQ ID N0:1539 -12.8 -20.6 63 -5.7 -2.1 -5.8 TGTGACGGTGAGGCTGGGGT

3758 SEQ ID N0:1540 -12.8 -28.5 80 -15.7 0 -3.7 ATTTAATTTCTCTTTGCTTT

3854 SEQ ID N0:1541 -12.8 -19.1 60.4 -6.3 0 -3.6 ACCAGATCTTCCCTTTGCCT

4029 SEQ ID N0:1542 -12.8 -29.1 79.8 -16.3 0 -5.8 GCATATTCTAGCATGGTTTT

4180 SEQ ID N0:1543 -12.8 -22.5 68.2 -9.2 -0.1 -5 AGAAATCTAGCCAGCACCAG

4284 SEQ ID N0:1544 -12.8 -24.1 68.3 -11.3 0 -4.6 TTTACTTTCACGTTTTTCCA

4648 SEQ ID N0:1545 -12.8 -22 65.6 -9.2 0 -4.7 GACCATTCCTTGGAATTTGT

5009 5EQ ID N0:1546 -12.8 -23.5 67.7 -9 -1.7 -8.4 GGTCATGTATTTGCCCTGGT

5117 SEQ ID N0:1547 -12.8 -27.7 79.1 -14.9 0 -4.7 GTTGGACATCCCAAAGATGG

5480 SEQ ID N0:1548 -12.8 -23.5 66.5 -8.4 -2.3 -8.3 GACACCTCCTCTTGTTTACG

6160 SEQ ID N0:1549 -12.8 -25.4 72 -12.6 0 -3 GAAGGAGGAGGGGTGGTAGA

6358 SEQ ID N0:1550 -12.8 -25 73.3 -12.2 0 -0.7 TTGGAAAGCATTTGACCTAA

7222 SEQ ID N0:1551 -12.8 -19.9 59 -6.3 -0.6 -4.1 GATTTAATAGAAGTTGTTTA

7444 SEQ ID N0:1552 -12.8 -15.8 52.5 -3 0 -2.5 AAGACTTGTGAAGAATGGAT

7595 SEQ ID N0:1553 -12.8 -17.9 55.6 -4.6 -0.2 -3.5 TTGAGTGTTTGACCAATGTA

8087 SEQ ID N0:1554 -12.8 -21 63.5 -8.2 0 -3.4 CAGAGCCCAATAAGTGAAAT

8484 SEQ TD N0:1555 -12.8 -20.5 59.5 -7.7 0 -3.3 CCACCTTATATAATTTATTA

9075 SEQ ID N0:1556 -12.8 -18 55.8 -5.2 0 -5.1 TTAGGGTATAACGTGTCTTC

149 SEQ ID N0:1557 -12.7 -21.3 65.1 -8.6 0 -5.3 CCTGCACATTTAATTACGTG

444 SEQ ID N0:1558 -12.7 -21.5 62.3 -8.8 0 -5 TGGCTTTGGTTTGTTCTCAT

605 SEQ ID N0:1559 -12.7 -24.4 73.6 -11.7 0 -3.7 CTTATTGATATAGTAGGGAT

713 SEQ ID N0:1560 -12.7 -18.7 59.1 -6 0 -2.7 TTCCATGGATCACGAAGAAA

1024 SEQ ID N0:1561 -12.7 -20.2 58.9 -6.5 0 -10 TGAGACATTGCCCAGGTCCA

1091 SEQ ID N0:1562 -12.7 -28.2 77.6 -14.8 -0.5 -4.4 CCTCCATTCTTTAGCACCTT

1952 SEQ ID N0:1563 -12.7 -27 76 -14.3 0 -4.1 GCTATTGCGTCTTGGGGAAA

2171 SEQ ID N0:1564 -12.7 -24.5 69.4 -10.9 -0.8 -5.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTCGCTCTCCATGTCTGTGC

2313 SEQ ID N0:1565 -12.7 -29 83.9 -16.3 0 -4.3 GTGCATCTTCCCATTTGCTG

2387 SEQ ID N0:1566 -12.7 -27.1 77 -13.1 -1.2 -5.4 GTTTTACTTTTAACCATGCA

2703 SEQ ID N0:1567 -12.7 -21 63 -8.3 0 -5.2 GCCATAAAGAGGGTATTTAA

2785 SEQ ID N0:1568 -12.7 -19.9 59.6 -7.2 0 -3.7 AGACAATCCCTCCACATTTG

2996 SEQ ID N0:1569 -12.7 -24.3 68.3 -11.6 0 -2.9 GTTTGGCCAGCGACCTCCAT

3325 SEQ ID N0:1570 -12.7 -30.5 81.2 -17.1 -0.1 -8.8 ATAAGGCACATGGTTTGGCC

3337 SEQ ID N0:1571 -12.7 -25.2 71.6 -10.4 -2.l -6.2 AGCAAGGTTGTCTGAGCTAA

3431 SEQ ID N0:1572 -12.7 -23.1 69 -9.4 -0.9 -5.5 CTTTTTGGAAACACTCCCGC

3537 SEQ ID N0:1573 -12.7 -24.7 67.9 -10.9 -1 -4.6 ACTTTTGGCTTTCTAAAAAA

3559 5EQ ID N0:1574 -12.7 -17 53.5 -3.5 -0.6 -4.3 TTTGATAGTCTTTCGCTGTT

4163 SEQ ID N0:1575 -12.7 -22.4 68.1 -9.7 0 -3.4 CCCGTTGTCATGTTAACACA

4561 SEQ ID N0:1576 -12.7 -24.5 68.8 -11.1 -0.3 -8.7 ACTTTCACGTTTTTCCACCG

4645 SEQ ID N0:1577 -12.7 -25.1 69.8 -12.4 0 -4.7 CAACATTATCAAAGTTTACT

4662 SEQ ID N0:1578 -12.7 -16.6 53.2 -3.9 0 -4.1 ACATAATATCCATCCAGCCT

4722 SEQ ID N0:1579 -12.7 -24.5 68.9 -11.8 0 -3.2 TTGGAATTTGTTTGCTGGGC

5000 SEQ ID N0:1580 -12.7 -23.7 69.4 -11 0 -4.4 AGGATCATGATGCTGATATC

5056 SEQ ID N0:1581 -12.7 -20.9 63.7 -7.3 -0.6 -8.5 ATTTGCCCTGGTCATCCGTT

5109 SEQ ID N0:1582 -12.7 -28.9 79.1 -15 -1.1 -4.4 TGTATTTGCCCTGGTCATCC

5112 SEQ ID N0:1583 -12.7 -27.7 78.3 -15 0 -4.2 ATCATGCTGTTGCCAAAGGT

5551 SEQ ID N0:1584 -12.7 -24.6 70.4 -11.4 -0.1 -4.4 CCATAAACCTGTCTTCCATC

6090 SEQ ID N0:1585 -12.7 -24.2 68.6 -11.5 0 -2 ACCTCCTCTTGTTTACGTTT

6157 SEQ ID N0:1586 -12.7 -25.3 73.1 -12.6 0 -4.8 TGTTTTATAGGTAAGTCAAT

6277 SEQ ID N0:1587 -12.7 -17.9 57.5 -5.2 0 -2.3 TGGTTTTGTTACACTATCAT

6380 SEQ ID N0:1588 -12.7 -20.5 63.5 -7.8 0 -2.7 CTATCCCTATAGTCTAGGTA

6724 SEQ ID N0:1589 -12.7 -23.6 70.9 -l0 -0.8 -5.1 TAATCCTTTAAATATTAGAG

7133 SEQ ID N0:1590 -12.7 -14.9 49.7 -2.2 0 -4.7 ATAATCCTTTAAATATTAGA

7134 SEQ ID N0:1591 -12.7 -14.9 49.6 -2.2 0 -5.3 CTTTGTTCATGGCTAAAAAG

8268 SEQ ID N0:1592 -12.7 -18.3 56.5 -5.6 0 -4.7 ATTTTTTTTCTTTCTTTCTC

62 SEQ ID N0:1593 -12.6 -19.4 63.1 -6.8 0 -0.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATCCTTGACAGGTATCTCAT

276 SEQ ID N0:1594 -12.6 -23.4 70 -9.8 -0.9 -3.5 TTACGTGTAGCTTCTTGCAT

431 5EQ ID N0:1595 -12.6 -23.6 70 -9.4 -1.5 -7.2 TCTGCAGCACGTTTTTCGAT

544 SEQ ID N0:1596 -12.6 -24.7 70.5 -10.9 -0.9 -9.8 GCCTTTCCTTTATTCATTAC

745 SEQ ID N0:1597 -12.6 -23.3 69.1 -10.7 0 -2 TTCTAAGCAAAACCCTCTTG

995 SEQ TD N0:1598 -12.6 -21.3 61.8 -8.7 0 -4.1 GTTCCTCCATTCTTTAGCAC

1955 SEQ ID N0:1599 -12.6 -25.7 75.6 -13.1 0 -4.1 CGCCGTCTTCAAATGTGCTG

2265 SEQ TD N0:1600 -12.6 -25.6 70.1 -13 0 -3.6 CCAGAGGAATCCTCCAGCAT

2554 SEQ ID N0:1601 -12.6 -27.3 75 -12.5 -2.2 -11.2 TTCCAGAGGAATCCTCCAGC

2556 SEQ ID N0:1602 -12.6 -27.1 76 -12.6 -1.9 -10.4 TGACAGACCAAGCTCCATTA

2978 SEQ ID N0:1603 -12.6 -23.9 68 -11.3 0 -5 CGCGGAACACAATCAGGAAG

3267 SEQ ID N0:1604 -12.6 -21.8 61 -9.2 0 -6.4 TTCAGAACCACAAGGTTTCC

3382 SEQ ID N0:1605 -12.6 -23.2 67.1 -8.6 -2 -6.8 CTGAGCTAAATGAACTCAAT

3420 SEQ 2D N0:1606 -12.6 -17.9 55.1 -4 -1.2 -5.1 CTATCTTATTGCCTTCATGG

3588 SEQ ID NO:1607 -12.6 -22.9 67.9 -10.3 0 -4.7 TAAGCTCTTTGCTTATTTCA

3636 SEQ ID N0:1608 -12.6 -21 64.6 -5.7 -2.7 -6.9 CTTTGCCTTCTTCTGTACTT

4017 SEQ ID N0:1609 -12.6 -24.7 73.6 -12.1 0 -4.8 GATCTTCCCTTTGCCTTCTT

4025 SEQ ID N0:1610 -12.6 -27.7 78.8 -15.1 0 -4.1 TATCAAAGTTTACTTTCACG

4656 SEQ ID N0:1611 -12.6 -17.9 56 -3.9 -1.3 -7.1 TGGAATTTGTTTGCTGGGCG

4999 SEQ ID N0:1612 -12.6 -24.4 69.2 -11 -0.6 -4.9 CATGTATTTGCCCTGGTCAT

5114 SEQ ID N0:1613 -12.6 -26 74 -13.4 0 -4.2 TCCCAAAGATGGCATAGATA

5472 SEQ ID N0:1614 -12.6 -22 63.4 -8 -1.3 -5 GAAGGGCATCCATCTCTCCA

6063 SEQ ID N0:1615 -12.6 -27.6 77.4 -13.8 -1.1 -4.5 CTGGTTTTGTTACACTATCA

6381 SEQ ID N0:1616 -12.6 -21.4 65.6 -8.8 0 -2.7 AATACTTTAGATATATTTTT

7268 SEQ ID N0:1617 -12.6 -14.4 49.3 -1.8 0 -4.3 TAATAGAAGTTGTTTATCAG

7440 SEQ ID N0:1618 -12.6 -16.1 53.3 -3.5 0 -2.5 TACATCCCTGTTGGAAGCTT

7495 SEQ ID N0:1619 -12.6 -25.1 71.5 -11.4 -1 -7.4 TTTAACCAAGACTTGTGAAG

7602 SEQ ID N0:1620 -12.6 -18.3 56.3 -5.7 0 -4.9 AAAATTTATATCATGTTAGG

8307 SEQ ID NO:1621 -12.6 -14.8 49.6 -2.2 0 -5.2 TCACCATTCTTTTTAGCAAT

8418 SEQ ID N0:1622 -12.6 -21.3 63.9 -g.7 0 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GGAGTTTATATAATGCATTT

8876 SEQ ID N0:1623 -12.6 -18.3 57.6 -5 0 -8.8 AGGAGTTTATATAATGCATT

8877 SEQ ID N0:1624 -12.6 -18.2 57.5 -5 0 -8.6 ATCTCATCCCTGTCAAACCT

263 SEQ ID N0:1625 -12.5 -25.7 72.2 -13.2 0 -2 AATTATCTCATTTATTCTTA

351 SEQ ID N0:1626 -12.5 -16.6 54.7 -4.1 0 -2.3 GCCTGTGCCATCTTTTCATC

463 SEQ ID N0:1627 -12.5 -27.5 79 -15 0 -3 TCTCTAGTAAAAAGGCGGAA

511 SEQ ID N0:1628 -12.5 -19.3 57.8 -6.8 0 -4 AGGTTCTTTCCAGCTTCCAA

634 SEQ ID N0:1629 -12.5 -26.3 76.1 -13.1 -0.4 -4.8.

TGTTTTCAGTGCTCGGAGAA

1130 SEQ ID N0:1630 -12.5 -23.4 69.1 -10.3 -0.3 -6.4 AGCTTCTTTACCGACTGGAT

1192 SEQ ID N0:1631 -12.5 -24.5 70.1 -11.1 -0.7 -5.4 CAAACATTTATTCCTCAGAT

1286 SEQ 2D N0:1632 -12.5 -19.2 58.4 -6.7 0 -2.5 ATCGCTTGGGGGCCACTGCA

1304 SEQ ID N0:1633 -12.5 -30.9 82.5 -16.5 -1.9 -6.9 GCTTTCGCCGTCTTCAAATG

2270 SEQ ID N0:1634 -12.5 -25 69.4 -12.5 0 -3.2 GTTGCGTCGCTCTCCATGTC

2318 SEQ ID N0:1635 -12.5 -29 81.8 -15.9 -0.3 -6.9 CTGACTAACGTTACTGTTGC

2333 SEQ ID NO:1636 -12.5 -21.8 64.5 -8.8 -0.2 -7.6 TCCCATTTGCTGGAAGCCCT

2379 SEQ ID NO:1637 -12.5 -29.8 79.2 -15.1 -2.2 -5.6 TGGCTATGCTCACGGCTCTT

2580 SEQ ID N0:1638 -12.5 -27.7 78 -14 -1.1 ' -5.3 TTTTAACCATGCATCACAGC

2696 SEQ ID N0:1639 -12.5 -22.5 65.6 -10 0 -6.6 AATCCCAGTAAAGACCAGGT

2858 SEQ ID N0:1640 -12.5 -23.8 67.1 -11.3 0 -5.7 CAATAATTCCATCAAAGATA

2946 5EQ ID N0:1641 -12.5 -16.2 51.5 -3.7 0 -3.5 TGAATGATCGCAGTACAGAC

3012 SEQ ID N0:1642 -12.5 -20.8 61.9 -7.6 -0.4 -5.7 CTGTGACGGTGAGGCTGGGG

3759 SEQ TD N0:1643 -12.5 -28.2 78.4 -15.7 , 0 -3.7 AGATCTTCCCTTTGCCTTCT

4026 SEQ ID N0:1644 -12.5 -27.6 78.7 -15.1 0 -5.6 ATGGGTTTCTGAGGTTTCTT

4975 SEQ ID N0:1645 -12.5 -23.7 72.5 -11.2 0 -2.8 AGTAGTGTCTGAGGGAAACG

5202 SEQ ID N0:1646 -12.5 -21.8 64.9 -9.3 3 -3.4 CAGAATACTTTTCTATCATC

5295 SEQ ID N0:1647 -12.5 -18.2 58 -4.7 -0.9 -4.4 CATCAAAGCAAAGAGCAGCG

5399 SEQ ID N0:1648 -12.5 -21.2 60.8 -7.1 -1.6 -5.7 TGATGCCATAAACCTGTCTT

6095 SEQ ID N0:1649 -12.5 -23.1 66 -10.6 0 -3 TTGATGCCATAAACCTGTCT

6096 SEQ ID N0:1650 -12.5 -23.1 66 -10.6 0 -2.8 TCACTTCACTGTCTTGTATA

6605 SEQ ID N0:1651 -12.5 -21.7 67.4 -9.2 0 -2.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ACTATCCCTATAGTCTAGGT

6725 SEQ ID N0:1652 -12.5 -24.1 72.2 -10 -l.5 -7.8 TGGAAGTTAAAAATAGAATG

6800 5EQ ID N0:1653 -12.5 -12.9 45.1 0 0 -2.8 ATGGAAGTTAAAAATAGAAT

6801 SEQ ID N0:1654 -12.5 -12.9 45.1 0 0 -2.8 AATATGGCAGATATGGAAGT

6813 5EQ ID N0:1655 -12.5 -19.3 58.9 -5.4 -1.3 -5.2 ACACCTTTGTCTGATCATTT

6957 SEQ ID N0:1656 -12.5 -22.6 67.6 -10.1 0 -6.2 CAGGTTGAATTCATTCTATC

7639 SEQ ID N0:1657 -12.5 -19.8 61.8 -6.6 -0.4 -8.1 TTCTGCTTGACATAATGGAC

7663 SEQ ID N0:1658 -12.5 -20.7 62.4 -8.2 0 -3.6 TATTCTGCTTGACATAATGG

7665 SEQ ID N0:1659 -12.5 -19.6 60 -7.1 0 -3.6 AAAGTCCATGTTATATTTGT

8245 SEQ ID N0:1660 -12.5 -19 59.1 -6.5 0 -4.3 TCATTTATTCTTACAATATC

344 SEQ ID N0:1661 -12.4 -16.5 54.2 -4.1 0 -2.6 GTTTTCTTATTGATATAGTA

718 SEQ ID N0:1662 -12.4 -17.6 57.7 -5.2 0 -2.7 TTCAAAAGCAGAATCGCTTG

1316 SEQ ID N0:1663 -12.4 -19.5 58 -5.7 -1.3 -8.8 TGTATGTTTTCCCAGCAGCA

1653 SEQ ID N0:1664 -12.4 -26.5 76.2 -14.1 0 -5.4 ATATCATGTATGTTTTCCCA

1659 SEQ ID N0:1665 -12.4 -22.3 66.6 -9.9 0 -4.7 TCTCCTAACCCACCTACTCC

1891 SEQ ID N0:1666 -12.4 -28.8 77.6 -16.4 0 -0.2 GTTACTGTTGCGTCGCTCTC

2324 SEQ ID N0:1667 -12.4 -26.8 77.7 -13.8 -0.3 -6.9 TGCTGTGCATCTTCCCATTT

2391 SEQ ID NO:1668 -12.4 -27.1 77 -13.9 -0.6 -4.9 TGCTCACGGCTCTTTGCCTT

2574 SEQ ID N0:1669 -12.4 -29 80.3 -13.9 -2.7 -8.3 GCCAATGATCTTAATTAGCA

3080 SEQ ID N0:1670 -12.4 -21.1 62.3 -8.7 0 -4.4 GTGAGGTTTCCTAGAGCCCC

3109 SEQ ID N0:1671 -12.4 -29.8 83.3 -16.7 -0.5 -4.4 ATTCCAGTATTATTGGACAT

3616 SEQ ID N0:1672 -12.4 -20.7 62.9 -6.8 -1.4 -7.2 ACGGTGAGGCTGGGGTTGTT

3754 SEQ ID N0:1673 -12.4 -28.1 79.6 -15.7 0 -3.7 CCTTCTCGGGGTAGAACAAC

3907 SEQ ID N0:1674 -12.4 -24.3 68.7 -11.3 -0.3 -3.8 CCAGCGCCAACATTATCAAA

4669 SEQ ID N0:1675 -12.4 -23.5 64.3 -10.6 0 -7.6 GAAGTTTAACATCTCGTGAA

4755 SEQ ID N0:1676 -12.4 -18.8 57.7 -6.4 0 -3.9 TTTCTGTTCCTCTGTCATAA

4922 SEQ ID N0:1677 -12.4 -22.7 69.2 -10.3 0 -1.8 CTAGGGTCATGTATTTGCCC

5121 SEQ ID N0:1678 -12.4 -26.2 75.5 -12.3 -1.4 -6.9 GGAAACGAGCTTCAGCACAA

5189 SEQ ID N0:1679 -12.4 -22.6 64.1 -9.3 -0.7 -6.6 AAGAGCAGCGTGCGGATCCC

5389 SEQ ID N0:1680 -12.4 -29 77 -14.4 -2.2 -11.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CGGGATCAAACTTTTCCCAA

5859 SEQ ID N0:1681 -12.4 -23.3 64.4 -8.3 -2.6 -7.9 CCTCTTGTTTACGTTTCAAA

6153 SEQ ID N0:1682 -12.4 -21.5 63.5 -9.1 0 -4.8 TTTTATAGGTAAGTCAATCC

6275 SEQ ID N0:1683 -12.4 -19.1 59.8 -6.7 0 -2.5 TTTTTACTTTTGATTTTCTC

6458 SEQ ID NO:1684 -12.4 -18.1 58.8 -5.7 0 -2.2 ATAGTCTAGGTAGCCATTGG

6716 SEQ ID N0:1685 -12.4 -23.8 71.4 -10.8 -0.3 -5.6 GACTTTACAGGCACATAACT

7111 SEQ ID N0:1686 -12.4 -21.2 63.1 -8.8 0 -4 ACTTTAGATATATTTTTCTA

7265 SEQ ID NO:1687 -12.4 -16.4 54.4 -4 0 -4.3 ACTATCAGCTTTGCTGAGAG

7341 SEQ TD N0:1688 -12.4 -22.7 68.6 -6.7 -3.6 -12 AGACTATCAGCTTTGCTGAG

7343 SEQ ID N0:1689 -12.4 -22.7 68.6 -6.7 -3.6 -10.8 TTGAATTCATTCTATCACCA

7635 SEQ ID N0:1690 -12.4 -20.3 61.5 -7.2 -0.4 -8.4 GCTTGACATAATGGACAGAG

7659 5EQ ID N0:1691 -12.4 -20.6 61.8 -8.2 0 -3.3 TGCTTGACATAATGGACAGA

7660 SEQ ID N0:1692 -12.4 -20.6 61.5 -8.2 0 -3.6 ACCCATTATCTAACTTTTAT

8018 SEQ ID N0:1693 -12.4 -20.1 60.5 -7.7 0 -1.6 TTTGTTCATGGCTAAAAAGA

8267 SEQ TD N0:1694 -12.4 -18 55.9 -5.6 0 -4.7 TTATATCATGTTAGGTAATT

8302 SEQ ID N0:1695 -12.4 -17.1 55.6 -4.7 0 -4.7 ATAAGTGAAATGCAATAAAA

8475 SEQ ID N0:1696 -12.4 -12.8 44.6 0 0 -5.6 AATAAGTGAAATGCAATAAA

8476 SEQ ID N0:1697 -12.4 -12.8 44.6 0 0 -5.6 CTGCTTGGTGAATCATGCAC

8621 SEQ ID N0:1698 -12.4 -23.8 69.2 -9.4 -2 -6.5 TGTAGTACTGCTTGGTGAAT

8628 SEQ ID N0:1699 -12.4 -22 66.7 -9.1 0 -7.8 CTTATGTATACATAGGAGTT

8890 SEQ ID N0:1700 -12.4 -19 60.1 -5.1 -0.1 -10.9 GCTTTTTTTTTTTTTTTTTT

113 SEQ ID N0:1701 -12.3 -18.4 59.4 -6.1 0 -2.8 TGAATTATCTCATTTATTCT

353 SEQ ID N0:1702 -12.3 -17.4 56.2 -4.3 -0.6 -4.8 TGCAGCACGTTTTTCGATAG

542 SEQ ID N0:1703 -12.3 -23.1 66.8 -9.8 -0.9 -7.3 TTTTGGGCTTCTTGGCTTTC

567 SEQ ID N0:1704 -12.3 -25.4 75.7 -12.1 -0.9 -3.7 TTTCTTATTGATATAGTAGG

716 SEQ ID N0:1705 -12.3 -17.5 57.1 -5.2 0 -2.7 TTTTATAAGTGACTCAAAGG

968 SEQ ID N0:1706 -12.3 -16.8 54 -4.5 0 -4.7 CCCTCTTGCCAAGATTTTTA

983 SEQ ID N0:1707 -12.3 -24.8 70 -11.7 -0.6 -6.3 TTTCAAAAGCAGAATCGCTT

1317 SEQ 2D N0:1708 -12.3 -19.6 58.4 -5.7 -1.6 -9 TCGAGCATCTGCTGAAATTC

1801 SEQ ID N0:1709 -12.3 -22 64.5 -8.7 -0.9 -7.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CTTTTTAAGCTGTTCGAGCA

1814 SEQ ID N0:1710 -12.3 -22.6 66.8 -8 -2.3 -7.l TCCATCCATGGAGAAAAGGA

2081 SEQ ID N0:1711 -12.3 -22.3 63.6 -8.4 -1.4 -10.4 ACAATAATTCCATCAAAGAT

2947 SEQ ID N0:1712 -12.3 -16.7 52.4 -4.4 0 -3.3 TTGGCCAGCGACCTCCATAC

3323 SEQ ID N0:1713 -12.3 -29.1 77.6 -16.1 -0.1 -8.8 GGTTGTTTATGAATGACATA

3741 SEQ ID N0:1714 -12.3 -18.7 58.4 -5.5 -0.8 -6 CGGTGAGGCTGGGGTTGTTT

3753 SEQ ID NO:1715 -12.3 -28 79.4 -15.7 0 -3.7 CTTTGCTTTCTTCTAGTTCT

3843 SEQ ID N0:1716 -12.3 -23 71.7 -10.7 0 -4 ACCTTCTCGGGGTAGAACAA

3908 SEQ ID N0:1717 -12.3 -24.3 68.7 -11.1 -0.7 -4.7 TTTAAAGTCTTCTTCGGGTT

3944 SEQ ID N0:1718 -12.3 -21.4 64.9 -8.4 -0.5 -4 CAGATCTTCCCTTTGCCTTC

4027 SEQ ID N0:1719 -12.3 -27.4 77.8 -15.1 0 -5.8 GTTTTTCGAAGATTCCACCA

4045 SEQ ID N0:1720 -12.3 -23.4 67 -10.6 -0.1 -6.8 AGGTTTTTCGAAGATTCCAC

4047 SEQ ID N0:1721 -12.3 -21.9 64.9 -8.9 -0.4 -7.4 TGAAAGTCTCAAACCAGTTG

4086 SEQ ID N0:1722 -12.3 -19.6 59.2 -6.8 -0.1 -3.4 TATTTGCCCTGGTCATCCGT

5110 SEQ ID N0:1723 -12.3 -28.5 78.1 -15 -1.l -4.2 GTATTTGCCCTGGTCATCCG

5111 SEQ ID N0:1724 -12.3 -28.5 78.1 -15 -l.1 -4.4 ACATCCCAAAGATGGCATAG

5475 SEQ ID N0:1725 -12.3 -22.6 64.3 -8 -2.3 -8 GGCAAAGTTGGACATCCCAA

5486 SEQ ID N0:1726 -12.3 -24.7 68.3 -10.4 -2 -8 GGTCTCCCTTAACTGAGCTG

5673 SEQ TD N0:1727 -12.3 -26.5 75.6 -14.2 0 -5 TATAAACTGGGTCGCATCGG

5876 SEQ ID N0:1728 -12.3 -22.8 64.5 -10.5 0 -3.6 ATTTTTTAACCTTTGCTTTA

6218 SEQ ID N0:1729 -12.3 -19.4 59.8 -7.1 0 -3.6 TATTTTTTAACCTTTGCTTT

6219 SEQ ID N0:1730 -12.3 -19.4 59.8 -7.1 0 -3.6 ATCCTCCCTTTAATTGCCTC

6259 SEQ ID N0:1731 -12.3 -27 75.1 -14.7 0 -3 GAGGAGGGGTGGTAGAGGAA

6354 SEQ ID N0:1732 -12.3 -25 73.3 -12.7 0 -0.7 GTCACTTCACTGTCTTGTAT

6606 SEQ ID N0:1733 -12.3 -23.2 71.7 -10.9 0 -2.6 TTTGAGAGACAGGGTTCTTG

6940 SEQ ID N0:1734 -12.3 -22 67.2 -9.7 0 -3.6 ATTTGAGAGACAGGGTTCTT

6941 SEQ ID N0:1735 -12.3 -22 67.4 -9.7 0 -3.6 TACTTTTTGGAAAGCATTTG

7228 SEQ ID N0:1736 -12.3 -18.3 56.9 -4.4 -l.6 -5.5 TTTTCTAGAATGGATTTATT

7252 SEQ ID N0:1737 -12.3 -17 55.1 -4.2 0 -7.7 TTTGCTGAGAGCAGAAGATG

7332 SEQ ID N0:1738 -12.3 -21.1 63.6 -6.7 -2.1 -6.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-totalduplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TAGACTATCAGCTTTGCTGA

7344 SEQ ID N0:1739 -12.3-22.4 67.7 -6.7 -3.4 -10.5 GCTTGCAAAAGACACATACA

7479 SEQ ID N0:1740 -12.3-20.2 59.6 -5.8 -2.1 -6.9 CTGCTTGACATAATGGACAG

7661 SEQ ID N0:1741 -12.3-20.9 62.1 -8.6 0 -3.6 ATGTTATATTTGTAATAGCT

8238 SEQ ID N0:1742 -12.3-17.5 56.4 -5.2 0 -5.7 AAGTTGGAACATGTGAACTT

8701 SEQ ID N0:1743 -12.3-18.9 57.9 -5.6 -0.8 -9.3 CTGAAAGTTGGAACATGTGA

8705 SEQ ID N0:1744 -12.3-19.2 58.2 -6.1 -0.6 -7 GTAGGTGGTTACTACTATTA

8734 SEQ ID N0:1745 -12.3-20.9 65.4 -7.6 -0.9 -4.4 TTTTCTTTCTTTCTCTGTGG

57 SEQ ID N0:1746 -12.2-22.3 70 -10.1 0 -1.3 TTTTTTTTTTTTTTTTTGAC

107 SEQ TD N0:1747 -12.2-16.4 54.6 -4.2 0 -0.1 CTTTTTTTTTTTTTTTTTTT

112 SEQ ID N0:1748 -12.2-16.7 55.4 -4.5 0 0 TGGTTAGGGTATAACGTGTC

l52 5EQ ID N0:1749 -12.2-22.3 67.1 -8.1 -2 -5.3 CTTCTTGCATACGAATTACC

421 SEQ ID N0:1750 -12.2-21.5 62.7 -9.3 0 -4.8 CAGCACGTTTTTCGATAGCA

540 SEQ ID N0:1751 -12.2-23.8 68 -9.8 -1.8 -4.1 ATCATCATTATCTTGTTCCT

587 SEQ ID N0:1752 -12.2-22 67.3 -9.8 0 -1.9 TGTGCCATTAAAGTAGGAAG

1346 SEQ ID N0:1753 -12.2-20.1 60.4 -7.9 0 -3.5 TTTCCACAGAGTAAAGGGTC

1468 SEQ ID N0:1754 -12.2-22.3 66.8 -10.1 0 -3.6 AATGCTTGTTTTGCTATTGC

2183 SEQ ID N0:1755 -12.2-21.9 65.9 -9 -0.4 -4.4 TCCATGTCTGTGCGGCACAA

2306 SEQ ID N0:1756 -12.2-27.3 75.3 -12.5 -1.4 -13.2 CCCAGATCAAGAACACATTG

2673 SEQ ID N0:1757 -12.2-21.5 61.8 -9.3 0 -5.4 ATGGCCATAAAGAGGGTATT

2788 SEQ ID N0:1758 -12.2-22 64.2 -8.8 0 -10 GTGCTTCCTTCAGATGAGCT

3880 SEQ ID N0:1759 -12.2-26.4 77.5 -12.8 -1.3 -5 CTTTTTAATACACCCTTCAG

3980 SEQ ID N0:1760 -12.2-21.2 62.8 -9 0 -2.5 ACAACCACCCTCATGCCTTC

4426 SEQ ID N0:1761 -12.2-28.5 76.3 -16.3 0 -4.4 TTCACAACCACCCTCATGCC

4429 SEQ TD N0:1762 -12.2-28.3 75.5 -16.1 0 -4.4 ACCCGTTGTCATGTTAACAC

4562 SEQ ID N0:1763 -12.2-24 68.2 -11.1 -0.3 -8.7 CATAATATCCATCCAGCCTT

4721 SEQ ID N0:1764 -12.2-24.4 68.6 -12.2 0 -3.2 CTTAACTGAGCTGCCAGGGT

5666 SEQ ID N0:1765 -12.2-26.5 75 -13.8 0.3 -8.4 ATCCCAACAGATGGGTCCCC

5695 SEQ ID N0:1766 -12.2-29.9 79 -14.5 -3.2 -8.6 CTATAAACTGGGTCGCATCG

5877 SEQ ID N0:1767 -12.2-22.5 63.9 -10.3 0 -3.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mo1 C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGGCTCATAAGAGACTTTGG

6122 SEQ ID N0:1768 -12.2 -21.7 65.4 -8.l -1.3 -5 TATGGAAGTTAAAAATAGAA

6802 SEQ ID N0:1769 -12.2 -12.6 44.6 0 0 -2.8 GTATTTAATTGACATAGACT

7358 SEQ ID N0:1770 -12.2 -17.1 55 -4.9 0 -2.9 GGTATTTAATTGACATAGAC

7359 SEQ ID NO:1771 -12.2 -17.4 55.6 -5.2 0 -2.9 ATGATACAGGATACATCCCT

7506 SEQ ID N0:1772 -12.2 -22.9 66.2 -9.7 -0.9 -6.9 ACAAAGTCCATGTTATATTT

8247 SEQ ID N0:1773 -12.2 -18.7 58 -6.5 0 -4.3 GGAACATGTGAACTTGATCC

8696 SEQ ID N0:1774 -12.2 -21.3 62.7 -9.1 0 -7 TGTAGGTGGTTACTACTATT

8735 SEQ TD N0:1775 -12.2 -21.2 65.9 -7.4 -1.6 -5.2 TTTTTTTTTTTTTTTTTTGA

108 SEQ ID N0:1776 -12.1 -16.3 54.4 -4.2 0 -0.1 GTATAACGTGTCTTCCTCTG

144 SEQ ID N0:1777 -12.1 -23.3 69.1 -11.2 0 -4.8 AGTGCCTGTGCCATCTTTTC

466 SEQ ID NO:1778 -12.1 -27.6 79.9 -14.7 -0.6 -3.4 CTCTAGTAAAAAGGCGGAAG

510 SEQ ID N0:1779 -12.1 -18.9 56.7 -6.8 0 -4 GTTTGTTCTCATCATCATTA

597 SEQ ID N0:1780 -12.1 -21.1 66.4 -9 0 -1.9 ACTATTTGGCTTTGGTTTGT

611 SEQ ID N0:1781 -12.1 -22.9 69.3 -10.8 0 -3.7 AATCGGAAAATTGCCTTTCC

757 SEQ ID N0:1782 -12.1 -21.6 61.2 -7.8 -1.7 -6.6 ATCTTGATAGCAATTTTCCT

823 SEQ ID N0:1783 -12.1 -21.1 63.7 -8.4 -0.3 -0.5 AACCCTCTTGCCAAGATTTT

985 SEQ ID N0:1784 -12.1 -24.5 68.5 -11.7 -0.4 -6.1 TATCCTTCTGGACACTGGCC

1501 SEQ ID N0:1785 -12.1 -27.2 76.6 -13.7 -1.3 -8.7 ATAGGCCATGGCCACCACAG

1733 SEQ ID N0:1786 -12.1 -28.9 77.5 -12.2 -2.5 -17.4 GCCTGAGCTTCTTCCTGTTG

1834 SEQ ID N0:1787 -12.1 -28.1 80.7 -15.3 -0.4 -4.6 TTTCGCCGTCTTCAAATGTG

2268 SEQ ID N0:1788 -12.1 -23.5 66.6 -11.4 0 -2.8 GGGGAAGTTGTCCAGTAGGT

2466 SEQ ID N0:1789 -12.1 -26.4 77.7 -12.3 -2 -5.6 CACATTGGCAAATCTATACC

2660 SEQ ID N0:1790 -12.1 -20.9 61.3 -8.8 0 -4.4 ACTTTTAACCATGCATCACA

2698 SEQ ID N0:1791 -12.1 -21.8 63.8 -9.7 0 -6.6 ATAATTCCATCAAAGATATT

2944 SEQ ID N0:1792 -12.1 -16.3 52.2 -3.7 -0.2 -3.5 AAGGAGTGGAAGAAGTCGTT

3250 SEQ ID N0:1793 -12.1 -21 62.8 -8.4 -0.2 -3 CCCGCATCTTATTTTTCACA

3522 5EQ ID N0:1794 -12.1 -24.8 69.7 -12.7 0 -3.6 CATTTAATTTCTCTTTGCTT

3855 SEQ ID N0:1795 -12.1 -19.7 61.3 -7.6 0 -3.6 GCTCTTAGAGGTCTTAAAGC

4393 SEQ ID N0:1796 -12.1 -22.3 68.1 -9.6 -0.3 -5.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCCACCGAGCTTGCTTGCCA

4632 5EQ ID N0:1797 -12.1 -30.8 81.3 -18.2 -O.l -5.9 TTCCACCGAGCTTGCTTGCC

4633 SEQ ID N0:1798 -12.1 -30.2 80.7 -17.5 -0.3 -6.4 TTATCAAAGTTTACTTTCAC

4657 SEQ ID N0:1799 -12.1 -17.2 55.6 -3.9 -1.1 -6.9 GTTTAACATCTCGTGAATCA

4752 SEQ ID N0:1$00 -12.1 -20 60.7 -7.9 0 -4.4 CATCATGGTGACCATGTTGA

5084 SEQ ID N0:1801 -12.1 -23.6 68.5 -8.3 -3.2 -11.5 ATAAACTGGGTCGCATCGGG

5875 SEQ ID N0:1802 -12.1 -24.3 67.4 -12.2 0 -3.6 CTGGACTTTGTTGGGTTTTG

5951 SEQ ID N0:1803 -12.1 -23.2 69.5 -11.1 0 -2.6 TATTCGAAGGGCATCCATCT

6068 SEQ TD N0:1804 -12.1 -24.2 68.9 -10.9 -1.1 -8.2 TCTGACCTCTTTTCCTTTGC

6440 SEQ ID N0:1805 -12.1 -25.9 75.6 -13.8 0 -2.6 GGAAAGCATTTGACCTAAAT

7220 SEQ ID N0:1806 -12.1 -19.1 57 -6.3 -0.4 -3.1 CATAGGGTATTTAATTGACA

7364 SEQ ID N0:1807 -12.1 -18.5 57.6 -6.4 0 -2.9 TGATTTAATAGAAGTTGTTT

7445 SEQ ID N0:1808 -12.1 -16.1 53 -4 0 -2.5 GGATACATCCCTGTTGGAAG

7498 SEQ ID N0:1809 -12.1 -24.1 68.9 -10.9 -1 -5.7 GGTTGAATTCATTCTATCAC

7637 SEQ ID N0:1810 -12.1 -20 62.2 -7.2 -0.4 -8.4 TAGGCAGTATCCAGTGTGTT

7769 SEQ ID N0:1811 -12.1 -25.1 75.6 -12.5 -0.2 -4 TTGTAATAGCTTTAGTGCAG

8229 5EQ ID N0:1812 -12.1 -20.3 63.1 -7.3 -0.8 -5.6 GTTATATTTGTAATAGCTTT

8236 SEQ ID N0:1813 -12.1 -17.7 57.2 -5.6 0 -7.1 GAAATTTATTCTTTTGGCTC

8452 SEQ ID N0:1814 -12.1 -19.1 59.6 -6.4 -0.3 -5.2 AGACCTTTCCAGAATCCTCT

23 SEQ ID N0:1815 -12 -25.5 72.6 -13.5 0 -2.9 AGCTTTCAGGTCCTGGGGGT

492 SEQ ID N0:1816 -12 -29.7 85.9 -17 -0.4 -6.8 CATCATCATTATCTTGTTCC

588 SEQ ID N0:1817 -12 -21.8 66.5 -9.8 0 -1.9 CTATTTGGCTTTGGTTTGTT

610 SEQ ID N0:1818 -12 -22.8 69.1 -10.8 0 -3.7 TGATATAGTAGGGATCCAGG

708 SEQ ID N0:1819 -12 -22.2 66.6 -9.4 -0.3 -8.9 TTGATATAGTAGGGATCCAG

709 SEQ ID N0:1820 -12 -21.1 64.3 -8.3 -0.3 -8.9 ATTGATATAGTAGGGATCCA

710 SEQ ID N0:1821 -12 -21.1 64 -8.3 -0.3 -8.9 TGGATCACGAAGAAACGTAA

1019 SEQ ID N0:1822 -12 -18.2 54.4 -5.3 -0.8 -6 TCAACGCTGAGACATTGCCC

1098 SEQ ID N0:1823 -12 -26.1 71.2 -14.1 0 -3.8 GGTTTCAAAAGCAGAATCGC

1319 SEQ ID N0:1824 -12 -21 61.5 -9 0 -5 GTTTCGACCAGCCTTCACAC

1526 SEQ ID N0:1825 -12 -27.1 75.6 -15.1 0 -4.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTCTTCAGATTCGGATTTGG

2042 SEQ ID N0:1826 -12 -22.8 68.2 -10.8 0 -4.4 TGCGTCTTGGGGAAAACAGG

2166 SEQ ID N0:1827 -12 -23.4 66 -11.4 0 -5.2 CGCTCTCCATGTCTGTGCGG

2311 SEQ ID N0:1828 -12 -29.4 80.3 -16.3 -1 -6.3 AGATCAAGAACACATTGGCA

2670 SEQ ID N0:1829 -12 -20.5 61 -8.5 0 -5.4 TTTAACCATGCATCACAGCA

2695 SEQ ID N0:1830 -12 -23.1 66.4 -10 -1 -6.6 TGTTTTACTTTTAACCATGC

2704 SEQ ID N0:1831 -12 -20.3 61.6 -8.3 0 -4.2 CCAGTAAAGACCAGGTTTCC

2854 SEQ ID N0:1832 -12 -24.7 70 -12.7 0 -5.9 TCCCAGTAAAGACCAGGTTT

2856 SEQ ID N0:1833 -12 -24.7 70 -12.7 0 -5.9 AAGCAGTCTGAATGATCGCA

3020 SEQ ID N0:1834 -12 -22.5 65.3 -9.8 -0.4 -6.3 AATTTCTCTTTGCTTTCTTC

3850 SEQ ID N0:1835 -12 -21 65.9 -9 0 -3.6 CAGCACATTCATGATAGAGG

4469 SEQ TD N0:1836 -12 -21.4 64.2 -9.4 0 -6.4 TTTGTTTGCTGGGCGAGGTA

4994 SEQ ID NO:1837 -12 -26 74.8 -13.2 -0.6 -4.4 GAATTTGTTTGCTGGGCGAG

4997 SEQ ID N0:1838 -12 -23.8 68.3 -11 -0.6 -4.9 CAAAGTTGGACATCCCAAAG

5484 SEQ ID NO:1839 -12 -21 60.4 -7.3 -1.7 -5.2 ATCAATTCCAGCTTCCTTTT

5513 SEQ ID N0:1840 -12 -24 70 -12 0 -4.5 AGCTGCCAGGGTGAATTGTG

5658 SEQ ID N0:1841 -12 -26 74.1 -14 0 -5.6 TGGGTCGCATCGGGATCAAA

5869 SEQ TD N0:1842 -12 -25.2 69.3 -13.2 0 -6.9 TATGAGTATTTGTTAAAACA

6576 SEQ ID N0:1843 -12 -15.2 50.4 -3.2 0 -5.9 AGTGACATAGCATTATGAAT

6882 SEQ ID N0:1844 -12 -18.6 57.9 -5.9 -0.5 -4 GAGAGACAGGGTTCTTGTAT

6937 SEQ ID N0:1845 -12 -22.7 69.5 -10.7 0.2 -5 TGGAAAGCATTTGACCTAAA

7221 SEQ ID N0:1846 -12 -19.1 56.9 -6.3 -0.6 -4.1 CAAAGTCCATGTTATATTTG

8246 SEQ ID N0:1847 -12 -18.5 57.4 -6.5 0 -4.3 TGAAATTTATTCTTTTGGCT

8453 SEQ ID N0:1848 -12 -18.7 58.2 -5.6 -1 -5.2 GTGAAATGCAATAAAAAATG

8471 SEQ ID N0:1849 -12 -12.4 43.7 0 0 -5.6 AGTGAAATGCAATAAAAAAT

8472 SEQ ID N0:1850 -12 -12.4 43.7 0 0 -5.6 CTAGTTTGTTGTAAAATTCA

8602 SEQ ID N0:1851 -12 -17.3 55.5 -4.6 -0.4 -4.4 TCCTTGACAGGTATCTCATC

275 SEQ ID N0:1852 -11.9 -23.8 71.7 -10.9 -0.9 -3.5 GCTTTCAGGTCCTGGGGGTA

491 SEQ ID N0:1853 -11.9 -29.4 84.9 -17 -0.2 -6.2 ATTCTCTAGTAAAAAGGCGG

513 SEQ ID N0:1854 -11.9 -19.5 58.7 -7.6 0 -4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CACGTTTTTCGATAGCAGCA

537 SEQ ID N0:1855 -11.9 -23.8 68 -10.9 -0.9 -6.9 TTCTGCAGCACGTTTTTCGA

545 5EQ ID N0:1856 -11.9 -24.8 70.9 -11.7 -0.9 -9.8 GACATTGCCCAGGTCCACAA

1088 SEQ ID N0:1857 -11.9 -27.8 75.4 -15.9 0 -4 AAACATTTATTCCTCAGATT

1285 SEQ ID N0:1858 -11.9 -18.6 57.'5 -6.7 0 -2.5 CTGTGCGGCACAAACAGTGA

2299 SEQ ID N0:1859 -11.9 -24.6 68.6 -10.1 -1.5 -13.2 TCATCAGTAGCAGCAAGGTT

3442 SEQ ID N0:1860 -11.9 -23.9 71.8 -12 0 -5.4 TTTTGGAAACACTCCCGCAT

3535 SEQ ID N0:1861 -11.9 -24.4 66.9 -11.4 -1 -4.8 TAATTTCTCTTTGCTTTCTT

3851 SEQ ID N0:1862 -11.9 -20.3 63.6 -8.4 0 -3.6 TTAATTTCTCTTTGCTTTCT

3852 SEQ ID NO:1863 -11.9 -20.3 63.6 -8.4 0 -3.6 TTCAGCTTGTTCACCTTCTC

3920 SEQ ID NO:1864 -11.9 -25.3 76.2 -13.4 0 -4.5 TTAAAGTCTTCTTCGGGTTC

3943 SEQ ID N0:1865 -11.9 -21.7 66.1 -9.1 -0.5 -3.8 TGTCAGCATATTCTAGCATG

4185 SEQ ID N0:1866 -11.9 -22.1 67.3 -9.7 -0.1 -4.3 GCGCCAACATTATCAAAGTT

4666 SEQ ID N0:1867 -11.9 -22.1 62.9 -10.2 0 -6.4 TGGGTTTCTGAGGTTTCTTG

4974 SEQ ID N0:1868 -11.9 -23.7 72.4 -11.8 0 -2.8 TTTGCTGGGCGAGGTATGGG

4990 SEQ ID N0:1869 -11.9 -27 75.7 -14.3 -0.6 -4.4 GTTTGCTGGGCGAGGTATGG

4991 SEQ ID NO:1870 -11.9 -27 76.6 -14.3 -0.6 -4.4 TAGTGTCTGAGGGAAACGAG

5200 SEQ ID N0:1871 -11.9 -21.2 63.1 -9.3 3 -3.5 CAAAGCAAAGAGCAGCGTGC

5396 SEQ ID N0:1872 -11.9 -23.1 64.9 -9.6 -1.6 -7.2 CATGCTGTTGCCAAAGGTCT

5549 SEQ ID NO:1873 -11.9 -25.5 72.4 -12.7 -0.8 -4.4 AGCAGATCATGCTGTTGCCA

5556 SEQ ID N0:1874 -11.9 -26.7 76.4 -12.4 -2.4 -9.8 CTCACTCAGGGGCTCTGCAC

5813 SEQ ID N0:1875 -11.9 -28.5 82 -15.6 -0.9 -4.8 CAGGGCAGCTGCAAAATCAG

5912 SEQ ID N0:1876 -11.9 -23.8 67.5 -9 -0.7 -14 TCAATCCTCCCTTTAATTGC

6262 SEQ ID N0:1877 -11.9 -24.1 68.5 -11.6 -0.3 -3.8 AGTCAATCCTCCCTTTAATT

6264 SEQ ID N0:1878 -11.9 -23.5 68 -11.6 0 -2.5 AAGGAGGAGGGGTGGTAGAG

6357 SEQ ID N0:1879 -11.9 -24.4 72.2 -12.5 0 -0.7 TTTCCTTGTCTGGTTTTGTT

6390 SEQ ID N0:1880 -11.9 -24.1 73.4 -12.2 0 -3.2 TTTTCCTTGTCTGGTTTTGT

6391 SEQ ID NO:1881 -11.9 -24.1 73.4 -12.2 0 -3.2 TGACACATATACTTTACCTT

6521 SEQ ID N0:1882 -11.9 -20 60.6 -8.1 0 -2.4 AGTTGACACATATACTTTAC

6524 SEQ ID N0:1883 -11.9 -18.3 58 -5.9 -0.2 -3.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total dupleacTm target molecularmolecular of Positionoligo bindingformationDupleacstructureoligo oligo TGTAGTTACAATGTTCACTT

6751 SEQ ID N0:1884 -11.9 -19.7 61.7 -7.8 0 -5.6 AACTATGAATTGGGGACCAT

6861 SEQ ID N0:1885 -11.9 -21.2 61.5 -6.9 -2.4 -7.2 ATTACAATTCTTTTTCTGGT

7007 SEQ ID N0:1886 -11.9 -19.5 61.1 -7.6 0 -2.6 GAGACTTTACAGGCACATAA

7113 SEQ ID N0:1887 -11.9 -20.7 62.2 -8.8 0 -4 AAATATAGAGCTCTGAAACT

7199 SEQ ID N0:1888 -11.9 -16.9 53.6 -4 0 -10 TCTTTAACCAAGACTTGTGA

7604 SEQ ID N0:1889 -11.9 -20.3 61.2 -7.6 -0.6 -5.6 ACAAAAATTTATATCATGTT

8310 SEQ ID N0:1890 -11.9 -14.1 47.7 -2.2 0 -5.2 CACCATTCTTTTTAGCAATA

8417 SEQ ID N0:1891 -11.9 -20.6 61.9 -8.7 0 -4.1 TTTATTCTTTTGGCTCAATA

8448 SEQ ID N0:1892 -11.9 -19.6 61.1 -7.7 0 -4.9 GTATACATAGGAGTTTATAT

8885 SEQ ID N0:1893 -11.9 -17.8 57.5 -5.9 0 -6.1 GTCAAACCTTGATGTGGCTT

252 SEQ ID N0:1894 -11.8 -23.9 69 -10.9 -1.1 -5.7 TCTTTGCTCCTTTCCCAGTA

312 SEQ ID N0:1895 -11.8 -27.7 79.5 -15.9 0 -2.9 TATTCTTACAATATCCCTAG

339 SEQ ID N0:1896 -11.8 -19.8 60.3 -8 0 -3.2 TGTGAATTATCTCATTTATT

355 SEQ ID N0:1897 -11.8 -17.3 55.9 -5 -0.1 -3.2 GGCTTTGGTTTGTTCTCATC

604 SEQ ID N0:1898 -11.8 -24.8 75.7 -13 0 -3.7 AGTTGGTCAAAATAGTGCAC

876 SEQ ID N0:1899 -11.8 -20.3 61.9 -7.9 0 -8.6 TAAGTGACTCAAAGGTATAG

963 SEQ ID N0:1900 -11.8 -17.4 55.5 -5.6 0 -3.3 TCGGAGAACTCTGAATGTTC

1118 SEQ ID N0:1901 -11.8 -20.9 62.7 -8.4 -0.4 -7.9 AATTGTTTTCAGTGCTCGGA

1133 SEQ ID N0:1902 -11.8 -22.9 67.9 -11.1 0 -4.5 CTGTTTCCATCCATGGAGAA

2086 SEQ ID N0:1903 -11.8 -24.2 69.4 -10.8 -1.4 -10.4 GGGGAAAACAGGGAGCCACG

2158 SEQ ID N0:1904 -11.8 -25.5 68.5 -13.1 -0.3 -4.3 GCGTCTTGGGGAAAACAGGG

2165 SEQ ID N0:1905 -11.8 -24.6 68.5 -12.8 0 -3.5 GGAAGCCCTGGCACCATCCT

2368 SEQ ID N0:1906 -11.8 -31.7 82.6 -19 -0.7 -9.1 TCTGGGGGAAGTTGTCCAGT

2470 SEQ ID N0:1907 -11.8 -26.8 78 -13 -2 -8.1 CCTTCCAGAGGAATCCTCCA

2558 SEQ ID N0:1908 -11.8 -28.2 76.9 -14.2 -2.2 -11.2 AATAATTCCATCAAAGATAT

2945 SEQ ID N0:1909 -11.8 -15.5 50.2 -3.7 0 -3.5 CCCTCCACATTTGACAGACC

2989 SEQ ID N0:1910 -11.8 -27.4 74.3 -15.6 0 -2.4 GACAATCCCTCCACATTTGA

2995 SEQ ID N0:1911 -11.8 -24.9 69.3 -13.1 0 -3.2 GTTCAGAACCACAAGGTTTC

3383 SEQ ID N0:1912 -11.8 -22.4 66.6 -8 -2.6 -6.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GCTTTTTGGAAACACTCCCG

3538 SEQ ID N0:1913 -11.8 -24.7 67.9 -11.8 -1 -5.3 CCAACAGCAATTGGCACTGT

3775 SEQ ID N0:1914 -11.8 -24.8 69.2 -11.4 -1.5 -9.3 TTCCCTTTGCCTTCTTCTGT

4021 SEQ TD N0:1915 -11.8 -28.3 80.9 -16.5 0 -3 CTCTTAGAGGTCTTAAAGCT

4392 SEQ ID N0:1916 -11.8 -21.4 65.7 -9.6 0 -4.8 CTTGGAATTTGTTTGCTGGG

5001 SEQ ID N0:1917 -11.8 -22.8 67.1 -ll 0 -4.3 GGTGACCATGTTGAGGCAGA

5078 SEQ ID N0:1918 -11.8 -26.1 74.8 -13.8 -O.l -4.3 AAGATGGCATAGATAAACAT

5467 SEQ ID NO:1919 -11.8 -17.1 53.5 -5.3 0 -4 AGGGCATCCATCTCTCCACT

6061 SEQ ID N0:1920 -11.8 -28.8 81.3 -15.8 -1.1 -4 GGAGGAGGGGTGGTAGAGGA

6355 SEQ ID N0:1921 -11.8 -26.9 78.8 -15.1 0 -0.7 CAGTTGACACATATACTTTA

6525 SEQ ID N0:1922 -11.8 -18.8 58.8 -6.5 -0.2 -3.6 CTGTCTTGTATAGGCACTGA

6597 SEQ ID N0:1923 -11.8 -23.5 70.7 -ll -0.5 -4 AACACCTTTGTCTGATCATT

6958 SEQ ID N0:1924 -11.8 -21.8 65 -9.3 -0.5 -8.3 AAACACCTTTGTCTGATCAT

6959 SEQ ID N0:1925 -11.8 -21 62.5 -8.5 -0.5 -8.3 AATATAGAGCTCTGAAACTT

7198 SEQ TD N0:1926 -11.8 -17.7 55.7 -5 0 -9.7 CTTTTTGGAAAGCATTTGAC

7226 SEQ ID N0:1927 -11.8 -19.2 58.8 -6.5 -0.7 -4.6 ACTTTTTGGAAAGCATTTGA

7227 SEQ ID N0:1928 -11.8 -19.2 58.8 -5.8 -1.6 -5.5 GGATTGAGTAAAATAGAGCA

7579 SEQ TD N0:1929 -11.8 -18.1 56.3 -6.3 0 -4.1 GCACTAGTTTGTTGTAAAAT

8605 SEQ ID N0:1930 -11.8 -18.8 58.5 -6.3 -0.4 -6.3 CAAAGCTGCTTTTAATTTGC

8656 SEQ ID N0:1931 -11.8 -20 60.2 -6.7 -0.3 -10.9 GTTACAATTTTTTTTCTTTC

68 SEQ ID N0:1932 -11.7 -17.8 57.9 -6.1 0 -2.4 GGTATAACGTGTCTTCCTCT

145 SEQ ID N0:1933 -11.7 -24.5 71.9 -12.8 0 -5.3 TCTCATCCCTGTCAAACCTT

262 SEQ ID N0:1934 -11.7 -25.8 72.6 -14.1 0 -2 ATTACGTGTAGCTTCTTGCA

432 SEQ ID NO:1935 -11.7 -23.6 70 -10.3 -1.5 -7.1 AGCACGTTTTTCGATAGCAG

539 SEQ ID N0:1936 -11.7 -23.1 67.1 -9.6 -1.8 -5 TATAGTAGGGATCCAGGTCC

705 SEQ ID N0:1937 -11.7 -25.2 74.4 -12.2 -1.2 -8.9 AATCCAGCCAGTTCCATGGA

1035 SEQ ID N0:1938 -11.7 -27.1 75.1 -14 -1.3 -9.7 ATTAACAAATGTCCCATTTG

1373 SEQ ID N0:1939 -11.7 -19.1 57.2 -4.9 -2.5 -9.5 -CACGTAATGTCAACTGGTAA

1635 SEQ ID N0:1940 -11.7 -20 59.5 -8.3 0 -4.8 AAAATATCATGTATGTTTTC

1662 SEQ ID N0:1941 -11.7 -15.5 51.4 -3 -0.6 -6.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCATAGGCCATGGCCACCAC

1735 SEQ ID N0:1942 -11.7 -29.3 78.8 -13.4 -1.7 -16.6 TTCCATCCATGGAGAAAAGG

2082 SEQ ID N0:1943 -11.7 -21.8 62.7 -8.4 -1.7 -9.9 AACAGGGAGCCACGGATACT

2152 SEQ ID N0:1944 -11.7 -25.3 69.7 -13 -0.3 -3.7 CTTGGGGAAAACAGGGAGCC

2161 SEQ ID N0:1945 -11.7 -24.8 68.8 -13.1 0 -3.6 CGTCGCTCTCCATGTCTGTG

2314 SEQ ID NO:1946 -11.7 -28 78.7 -16.3 0 -4.3 CTGGGGGAAGTTGTCCAGTA

2469 SEQ ID N0:1947 -11.7 -26.1 75.6 -13 -1.3 -7.5 CTTTCTGACTTCCGTTTCAG

2501 SEQ TD N0:1948 -11.7 -23.9 70.4 -11.3 -0.8 -5.4 TCCAGAGGAATCCTCCAGCA

2555 SEQ ID N0:1949 -11.7 -27.7 76.7 -13.8 -2.2 -11.2 AATTCCATCAAAGATATTCC

2942 SEQ ID N0:1950 -11.7 -19 57.6 -6.6 -0.4 -3.9 TGCCAATGATCTTAATTAGC

3081 SEQ ID N0:1951 -11.7 -20.4 61.1 -8.7 0 -4.9 AATGAAGACGATGATGGCCA

3137 SEQ ID N0:1952 -11.7 -21.7 61.5 -9.3 0 -8.8 TCAGAACCACAAGGTTTCCA

3381 SEQ ID N0:1953 -11.7 -23.8 67.9 -9.5 -2.6 -7.6 TTAAGCTCTTTGCTTATTTC

3637 SEQ TD N0:1954 -11.7 -20.4 63.7 -5.7 -3 -7.4 TGACGGTGAGGCTGGGGTTG

3756 5EQ ID N0:1955 -11.7 -27.4 76.8 -15.7 0 -3.7 TTCTCTTTGCTTTCTTCTAG

3847 SEQ ID N0:1956 -11.7 -22.2 69.8 -10.5 0 -3.6 TTTAATTTCTCTTTGCTTTC

3853 SEQ ID N0:1957 -11.7 -19.5 61.9 -7.8 0 -3.6 ATTATCAAAGTTTACTTTCA

4658 SEQ ID N0:1958 -11.7 -17 55.1 -3.9 -1.3 -7.1 CATTATCAAAGTTTACTTTC

4659 SEQ ID N0:1959 -11.7 -17 55.1 -3.9 -1.3 -7.1 ACATTATCAAAGTTTACTTT

4660 SEQ ID N0:1960 -11.7 -16.8 54.3 -3.9 -1.1 -6.9 AGATTTTCTTCATATACAGG

4777 5EQ ID N0:1961 -11.7 -18.9 59.9 -7.2 0 -2.6 TGTACAGATTTTCTTCATAT

4782 SEQ ID N0:1962 -11.7 -18.9 60.1 -7.2 , 0 -5.9 ATGTACAGATTTTCTTCATA

4783 SEQ ID N0:1963 -11.7 -18.9 60.1 -7.2 0 -6.7 CATTCCTTGGAATTTGTTTG

5006 SEQ ID NO:1964 -11.7 -20.9 62.7 -7.5 -1.7 -8.4 AGACCATTCCTTGGAATTTG

5010 SEQ ID N0:1965 -11.7 -22.3 64.8 -9 -1.5 -8.2 CAGCCTATAGTGAAGTAGTA

5218 SEQ ID N0:1966 -11.7 -21.7 66 -10 0 -5.1 ACCATGGGCAGATCCATGGC

5977 SEQ ID N0:1967 -11.7 -28.5 78 -11.5 -5.3 -13.1 CTCCTCTTGTTTACGTTTCA

6155 SEQ ID N0:1968 -11.7 -24.2 71.6 -12.5 0 -4.8 CTTTTCCTTGTCTGGTTTTG

6392 SEQ ID N0:1969 -11.7 -23.8 71.9 -12.1 0 -3.2 CACATATACTTTACCTTCAT

6518 SEQ ID N0:1970 -11.7 -20.3 61.4 -8.6 0 -2.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Position oligo bindingformationDuplexstructureoligo oligo GTGACATAGCATTATGAATA

6881 SEQ ID N0:1971 -11.7 -18.3 57.1 -5.1 -1.4 -4.9 GATCATTTGAGAGACAGGGT

6945 SEQ ID N0;1972 -11.7 -22.2 67.1 -10.5 0 -4.7 GTTGGAAGCTTGCAAAAGAC

7486 SEQ ID NO:1973 -11.7 -20.4 60.6 -6.6 -2.1 -7 AGTGATAGCCTTTAAACTAT

7535 SEQ ID N0:1974 -11.7 -19.5 59.6 -7.3 -0.2 -5.8 CAATATTAACATGCATTAGT

7552 SEQ TD NO:1975 -11.7 -17.2 54.3 -5.5 0 -6.7 ACAGAGCAGGTTGAATTCAT

7645 SEQ ID N0:1976 -11.7 -21.6 65 -9.3 0 -8.4 ATGAAGAATAGGTTTTGTCA

7790 SEQ TD N0:1977 -11.7 -18.5 58.2 -6.8 0 -3 TAAGTGAAATGCAATAAAAA

8474 SEQ TD N0:1978 -11.7 -12.1 43.2 0 0 -5.6 CCTTATGTATACATAGGAGT

8891 SEQ ID N0:1979 -11.7 -20.9 63.7 -7.6 -0.2 -ll.l TTTTTTTTTTTGACCACAGA

101 SEQ ID N0:1980 -11.6 -20 61.2 -8.4 0 -2.3 TTTTTTTTTTTTTTTTTTTG

109 SEQ ID N0:1981 -11.6 -15.8 53.3 -4.2 0 0 CTTGCATACGAATTACCTTA

418 SEQ ID N0:1982 -11,6 -20.8 60.8 -9.2 0 -5.1 TGCCTGTGCCATCTTTTCAT

464 SEQ TD N0:1983 -11.6 -27.1 77 -14.7 -0.6 -3 AAGGTTCTTTCCAGCTTCCA

635 SEQ ID N0:1984 -11,6 -26.3 76.1 -14 -0.4 -4.8 AGGGATCCAGGTCCTCCAGG

699 SEQ ID N0:1985 -11,6 -30.1 83.9 -16.5 -2 -10.3 CACAGTTGGTCAAAATAGTG

879 SEQ ID N0:1986 -11.6 -19.2 59 -7.6 0 -3.8 AAATGTCCCATTTGAATCCA

1367 SEQ ID NO:1987 -11.6 -22.1 62.9 -9.2 -1.2 -5.7 TGTTACATTAACAAATGTCC

1379 SEQ ID N0:1988 -11.6 -18.1 56.1 -4.9 -1.5 -6.6 GCCGCAACTGCCTGAGCTTC

1843 SEQ ID N0:1989 -11.6 -30 80.1 -17.2 -1.1 -5.8 TCAAGGTGCTCTCTCTGTCT

1990 SEQ ID N0:1990 -11.6 -25.9 78.7 -14.3 0 -3.6 TCTGTTTCCATCCATGGAGA

2087 SEQ ID NO:1991 -11.6 -25.3 73.4 -12 -1.7 -10.4 CTGATGAGGGGAGCAGAATT

2123 SEQ TD N0:1992 -I1.6 -22.8 66.7 -11.2 0 -4.1 CATTTGCTGGAAGCCCTGGC

2376 SEQ ID N0:1993 -11.6 -28.4 77.3 -15.2 -1.5 -8.6 CGGCTCTTTGCCTTCCAGAG

2568 SEQ ID N0:1994 -11.6 -28.7 79.I -14.4 -2.7 -7.3 TAATTCCATCAAAGATATTC

2943 SEQ ID N0:1995 -11.6 -16.7 53.4 -4.4 -0.4 -3.5 GGTGAGGTTTCCTAGAGCCC

3110 SEQ ID N0:1996 -11.6 -29 82.4 -16.7 -0.5 -4.4 ATGAAGACGATGATGGCCAA

3136 SEQ TD N0:1997 -11.6 -21.7 61.5 -9.3 0 -9.2 AGGTTTCCAATGACCATGAC

3370 SEQ ID N0:1998 -11.6 -23.3 67.1 -10.1 -1.5 -5.8 CCGCATCTTATTTTTCACAT

3521 SEQ ID N0:1999 -11.6 -22.8 66.1 -11.2 0 -3.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GAGGCTGGGGTTGTTTATGA

3749 SEQ ID N0:2000 -11.6 -25.1 74.1 -13.5 0 -3.7 GCTAACCAAAGAAACATCAA

4310 SEQ ID N0:2001 -11.6 -17.7 53.6 -6.1 0 -2.9 ATAATATCCATCCAGCCTTT

4720 SEQ ID N0:2002 -11.6 -23.8 67.9 -12.2 0 -3.2 AACGAGCTTCAGCACAAATT

5186 SEQ ID N0:2003 -11.6 -20.9 60.8 -9.3 0.1 -5.8 AAGAGCAGGAGGCCGATGTT

5440 SEQ ID N0:2004 -11.6 -25.9 72.4 -13.4 -0.8 -7.7 TCATGCTGTTGCCAAAGGTC

5550 SEQ ID N0:2005 -11.6 -25 72.1 -12.5 -0.8 -4.4 TAAACTGGGTCGCATCGGGA

5874 SEQ ID N0:2006 -11.6 -24.9 68.6 -13.3 0 -3.6 GAAGAGGAGGATCCAGGGCA

5925 SEQ ID N0:2007 -11.6 -26.1 73.9 -12.7 -1.8 -9 CTGTTTTTTCTGGAGTGGAG

6327 5EQ ID N0:2008 -11.6 -22.7 69.5 -11.1 0 -2.7 AGGAGGGGTGGTAGAGGAAC

6353 SEQ ID N0:2009 -11.6 -24.6 72.6 -13 0 -1.9 ACATATACTTTACCTTCATA

6517 SEQ ID N0:2010 -11.6 -19.3 59.6 -7.7 0 -2.4 CCAGTTGACACATATACTTT

6526 SEQ ID N0:2011 -11.6 -21.1 63.2 -9 -0.2 -3.6 AGCCATTGGGTTTCTCCTCA

6705 SEQ ID N0:2012 -11.6 -28.1 80.5 -15.6 -0.8 -6.3 AGCTTGCAAAAGACACATAC

7480 SEQ ID N0:2013 -11.6 -19.5 58.6 -5.8 -2.1 -6.9 ATATTAACATGCATTAGTGA

7550 SEQ ID N0:2014 -11.6 -17.8 56.2 -6.2 0 -6.7 GATTGAGTAAAATAGAGCAG

7578 SEQ ID N0:2015 -11.6 -16.9 54 -5.3 0 -4.1 TCAGTAGGCAGTATCCAGTG

7773 SEQ ID N0:2016 -11.6 -24.9 75 -12.8 -0.2 -4 TTGTTCATGGCTAAAAAGAA

8266 SEQ ID N0:2017 -11.6 -17.2 53.8 -5.6 0 -4.1 TCTTTTTAGCAATAGCAATT

8411 SEQ 2D N0:2018 -11.6 -18.8 58.6 -5.5 -1.7 -4.6 AAATTTATTCTTTTGGCTCA

8451 SEQ ID N0:2019 -11.6 -19.2 59.6 -7.6 0 -4.3 TTATGTATACATAGGAGTTT

8889 SEQ TD N0:2020 -11.6 -18.2 58.4 -5.1 -0.1 -10.9 CATTAATACCTTATGTATAC

8899 SEQ ID N0:2021 -11.6 -17.2 54.7 -5 -0.3 -5.3 TTTTTTTTTTTTTTTTTTCC

9093 SEQ ID N0:2022 -11.6 -18.1 58.6 -6.5 0 0 ATTTGGCTTTGGTTTGTTCT

608 SEQ ID N0:2023 -11.5 -23.5 71.4 -12 0 -2.9 GCCTTCACACAGATGTATCC

1516 SEQ ID N0:2024 -11.5 -25.6 73.6 -7:3.4 -0.5 -3.7 GCTTCTTCCTGTTGCTTTTT

1828 SEQ ID N0:2025 -11.5 -25.9 77 -14.4 0 -3.6 CTCCACTGAAATCTCTTGAA

1875 SEQ ID N0:2026 -11.5 -20.9 61.8 -9.4 0 -2.5 TCAACTTTGATGCTTCTGAA

1926 SEQ ID N0:2027 -11.5 -20.3 61.5 -8.8 0 -5 CCATCCATGGAGAAAAGGAA

2080 SEQ ID N0:2028 -11.5 -21.2 60.4 -8.4 -0.4 -10.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GAGCCACGGATACTCAAGAG

2146 SEQ ID N0:2029 -11.5 -23.7 67.3 -11.5 -0.4 -4.9 TCTTTCTGACTTCCGTTTCA

2502 SEQ ID N0:2030 -11.5 -24.3 71.7 -12.8 0 -3.8 CTGGCTATGCTCACGGCTCT

2581 SEQ ID N0:2031 -11.5 -28.5 79.6 -15.8 -1.1 -5.3 TGAGCTAAATGAACTCAATA

3419 SEQ ID N0:2032 -11.5 -16.7 52.8 -4 -1.1 -5.1 CGCATCTTATTTTTCACATA

3520 SEQ ID N0:2033 -11.5 -20.5 61.8 -9 0 -3.6 CATCAACGATCAAGAAATCT

4296 SEQ ID N0:2034 -11.5 -17.6 54 -6.1 0 -4.9 AGTGCAAGATAGCCAGCGCC

4681 SEQ ID N0:2035 -11.5 -28.2 76.8 -15.5 -1.1 -7.7 TTTTCTTCATATACAGGCTG

4774 5EQ ID N0:2036 -11.5 -21 64.6 -9.5 0 -3.8 CGTAGGATTCGGCCAATCCT

5350 SEQ ID N0:2037 -11.5 -27.1 72.9 -11.9 -3.3 -15.2 CCCAAAGATGGCATAGATAA

5471 SEQ TD N0:2038 -11.5 -20.9 60.2 -8 -1.3 -5 GTCACCACTGACCATGGGCA

5987 SEQ ID N0:2039 -11.5 -28.7 78.5 -15.7 -1.4 -8.9 GTGGATCCGGTCACCACTGA

5996 SEQ ID N0:2040 -11.5 -28.3 77.6 -14.5 -2.2 -12 TGCCATAAACCTGTCTTCCA

6092 SEQ ID N0:2041 -11.5 -25.6 71 -14.1 0 -3 AGGAGGAGGGGTGGTAGAGG

6356 SEQ ID N0:2042 -11.5 -26.3 77.7 -14.8 0 -0.7 ATCATAGGAAGGAGGAGGGG

6365 SEQ ID N0:2043 -11.5 -23.1 68 -11.6 0 -3.4 CTTTCTTTTTCTGGTTTGTC

6421 SEQ ID N0:2044 -11.5 -22.3 70.2 -10.8 0 -1.5 AATAAACTATGAATTGGGGA

6865 5EQ ID N0:2045 -11.5 -16.6 52.2 -5.1 0 -3.1 GAATAAACTATGAATTGGGG

6866 SEQ ID N0:2046 -11.5 -16.6 52.2 -5.1 0 -3.1 AGACTTTACAGGCACATAAC

7112 SEQ ID N0:2047 -11.5 -20.3 61.4 -8.8 0 -4 GGCAATATTAACATGCATTA

7554 SEQ ID N0:2048 -11.5 -19 57.7 -5.5 -2 -6.8 CATTCTATCACCAATATGTG

7628 SEQ ID N0:2049 -11.5 -20 60.5 -8.5 0 -3.8 AGGCAGTATCCAGTGTGTTT

7768 SEQ TD N0:2050 -11.5 -25.5 76.7 -13.5 -0.2 -4 GTTTTAGACATATTTTTAGC

7815 5EQ ID N0:2051 -11.5 -18.3 58.9 -6.8 0 -2.8 TATTTAAACAAGTTTTAGAC

7826 SEQ ID N0:2052 -11.5 -14.4 49 -2.9 0 -5.2 CCAGAGCCCAATAAGTGAAA

8485 SEQ ID N0:2053 -11.5 -22.5 62.9 -11 0 -2.4 ACTAGTTTGTTGTAAAATTC

8603 SEQ ID N0:2054 -11.5 -16.8 54.7 -4.6 -0.4 -6 TGAAAGTTGGAACATGTGAA

8704 SEQ ID N0:2055 -11.5 -17.6 54.6 -5.3 -0.6 -7 ATGTATACATAGGAGTTTAT

gg87 SEQ ID N0:2056 -11.5 -18.1 58 -5.9 0 -9.1 TCTTTCTTTCTCTGTGGATA

54 SEQ ID N0:2057 -11.4 -22.3 69.6 -10.9 0 -2.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TACAATTTTTTTTCTTTCTT

66 SEQ ID N0:2058 -11.4 -17.5 56.8 -6.1 0 -2.6 TTACAATTTTTTTTCTTTCT

67 SEQ ID N0:2059 -11.4 -17.5 56.8 -6.1 0 -2.6 TTTGGGCTTCTTGGCTTTCT

566 SEQ ID N0:2060 -11.4 -26.2 77.4 -14.3 -0.1 -3.7 AATCTTGATAGCAATTTTCC

824 SEQ ID N0:2061 -11.4 -19.5 59.7 -7.5 -0.3 -0.5 CTTCTTTACCGACTGGATCA

1190 SEQ ID N0:2062 -11.4 -23.8 68.4 -11.5 -0.7 -5.3 GCAGAATCGCTTGGGGGCCA

1309 SEQ ID N0:2063 -11.4 -29.7 79.3 -17.6 -0.5 -7.6 TTGTGCCATTAAAGTAGGAA

1347 SEQ ID N0:2064 -11.4 -20.2 60.5 -8.8 0 -3.5 ATGTATGTTTTCCCAGCAGC

1654 SEQ ID N0:2065 -11.4 -25.8 75 -14.4 0 -4.1 GCTGCTTCTTTTGACGCTGT

2060 SEQ ID N0:2066 -11.4 -26.6 76.3 -13.2 -2 -6.3 TGTTCATCATCAGCAAAGTC

2245 SEQ ID N0:2067 -11.4 -21.3 65.3 -9.9 0 -4.1 ATTCCATCAAAGATATTCCA

2941 SEQ ID N0:2068 -11.4 -20.4 60.7 -8.3 -0.4 -3.9 AGCTAAATGAACTCAATAAT

3417 SEQ ID N0:2069 -11.4 -15.4 50 -4 0 -4.3 CTTATTGCCTTCATGGATTT

3584 SEQ ID N0:2070 -11.4 -22.7 67 -10.7 -0.3 -4.7 GGTGGTTCCATTCCCATCTC

3665 SEQ ID N0:2071 -11.4 -28.9 82.3 -16.8 -0.4 -5.5 CCAGATCTTCCCTTTGCCTT

4028 SEQ ID N0:2072 -11.4 -29 79.5 -17.6 0 -5.8 GAAAGTCTCAAACCAGTTGT

4085 SEQ ID N0:2073 -11.4 -20.8 62.2 -8.7 -0.4 -3.4 GCCAGCACCAGGCATTAGTG

4275 SEQ ID N0:2074 -11.4 -28.6 79.4 -15.3 -1.9 -7.5 CTCATGCCTTCAAACCGGGA

4417 SEQ ID N0:2075 -11.4 -26.5 71.2 -15.1 0 -7.1 AACATTATCAAAGTTTACTT

4661 SEQ ID N0:2076 -11.4 -16 52.2 -3.9 -0.4 -5.5 ACAGATTTTCTTCATATACA

4779 SEQ ID N0:2077 -11.4 -18.6 58.9 -7.2 0 -2.6 ATCATGGTGACCATGTTGAG

5083 SEQ ID N0:2078 -11.4 -22.9 67.6 -8.3 -3.2 -11.5 TAGTAGTGTCTGAGGGAAAC

5203 SEQ ID N0:2079 -11.4 -20.7 64 -9.3 0 -3.2 AGTTGGACATCCCAAAGATG

5481 SEQ ID N0:2080 -11.4 -22.3 64.3 -8.7 -2.2 -8 CCAGCAGAGGTTGTAATTTG

5581 SEQ ID N0:2081 -11.4 -22.7 67.1 -11.3 0 -5.7 ATCCCAGCCAGCAGAGGTTG

5588 SEQ TD N0:2082 -11.4 -29.2 80.9 -17.1 -0.5 -4.9 AGTCATCCTCACTCAGGGGC

5820 SEQ ID N0:2083 -11.4 -28.3 82.6 -16 -0.8 -7.6 GATGCCATAAACCTGTCTTC

6094 SEQ TD N0:2084 -11.4 -23.5 67.6 -12.1 0 -3 GCCTCTTTGTTATAGTTACT

6244 SEQ ID N0:2085 -11.4 -23 70.1 -11.6 0 -2.5 GTCAATCCTCCCTTTAATTG

6263 SEQ ID N0:2086 -11.4 -23.5 67.6 -11.6 -O.l -3.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTTCCCATTTAGTTTGTCAA

6308 SEQ ID N0:2087 -11.4 -23.3 69.4 -11.9 0 -2.7 ACTTTTCCTTGTCTGGTTTT

6393 SEQ ID N0:2088 -11.4 -24 72.7 -12.6 0 -3.2 AAACTATGAATTGGGGACCA

6862 SEQ ID N0:2089 -11.4 -20.5 59.7 -6.9 -2.2 -7.2 ATAAACTATGAATTGGGGAC

6864 SEQ ID N0:2090 -11.4 -17.5 54.4 -6.1 0 -3.1 CTTTACAGGCACATAACTGT

7109 SEQ ID N0:2091 -11.4 -21.6 64.3 -8.8 -1.3 -5 AATAATCCTTTAAATATTAG

7135 SEQ ID N0:2092 -11.4 -13.6 46.8 -2.2 0 -5.3 CTTGACATAATGGACAGAGC

7658 SEQ ID N0:2093 -11.4 -20.6 61.8 -9.2 0 -3.l TTATTCTGCTTGACATAATG

7666 SEQ ID N0:2094 -11.4 -18.5 57.8 -7.1 0 -3.6 TCTAACTTTTATTTTAGGAA

8010 5EQ ID N0:2095 -11.4 -16.5 53.8 -5.l 0 -3.2 CAACTTTGCCACTTTGTTCA

8279 SEQ ID N0:2096 -11.4 -23.5 68.2 -12.1 0 -3.1 CAAAAATTTATATCATGTTA

8309 SEQ ID N0:2097 -11.4 -13.6 46.8 -2.2 0 -5.2 AGCTCACCATTCTTTTTAGC

8421 SEQ ID N0:2098 -11.4 -24 71.7 -12.1 -0.1 -4.3 TGAAATGCAATAAAAAATGA

8470 SEQ ID N0:2099 -11.4 -11.8 42.6 0 0 -5.1 ACCTTTCCAGAATCCTCTCT

21 SEQ ID N0:2100 -11.3 -26.2 74.6 -14.9 0 -3.5 AGAGACCTTTCCAGAATCCT

25 SEQ 2D N0:2101 -11.3 -24.8 70.7 -13.5 0 -2.9 AGTTACAATTTTTTTTCTTT

69 SEQ ID NO:2102 -11.3 -17.4 56.7 -6.1 0 -2.6 CTGCACATTTAATTACGTGT

443 SEQ ID N0:2103 -11.3 -20.7 61.6 -8.8 -0.3 -5.2 AGTCACTATTTGGCTTTGGT

615 SEQ ID NO:2104 -11.3 -23.8 72 -11.8 -0.4 -3.7 GAACAGCTGCAGCCCAATGA

1259 SEQ ID N0:2105 -11.3 -26.4 71.9 -13 0 -12.4 GTAAAGATTTTCCCAATAGT

1619 SEQ ID N0:2106 -11.3 -19.7 59.6 -8.4 0 -3.2 TCCTAACCCACCTACTCCAC

1889 SEQ ID N0:2107 -11.3 -28.4 75.7 -17.1 0 -0.3 CAAGGTGCTCTCTCTGTCTT

1989 SEQ ID N0:2108 -11.3 -25.6 77.2 -14.3 0 -3.6 GATCAAGAACACATTGGCAA

2669 SEQ ID N0:2109 -11.3 -19.8 58.9 -8.5 0 -4.7 CCCAGTAAAGACCAGGTTTC

2855 SEQ ID N0:2110 -11.3 -24.7 70 -13.4 0 -5.9 GAGCTAAATGAACTCAATAA

3418 SEQ ID NO:2111 -11.3 -16 51.2 -4 -0.4 -5.1 TTCAATTCCAGTATTATTGG

3620 SEQ ID N0:2112 -11.3 -19.7 60.7 -7.7 -0.4 -5 ATTAAGCTCTTTGCTTATTT

3638 SEQ ID N0:2113 -11.3 -20 62.2 -5.7 -3 -7.5 , TGAGGCTGGGGTTGTTTATG

3750 SEQ ID N0:2114 -11.3 -24.5 72.5 -13.2 0 -3.7 TTCACGTTTTTCCACCGAGC

4642 SEQ ID N0:2115 -11.3 -26.3 72.6 -15 0 -4.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TAATATCCATCCAGCCTTTA

4719 SEQ ID N0:2116 -11.3 -23.5 67.4 -12.2 0 -3.2 CCTTGGAATTTGTTTGCTGG

5002 SEQ ID N0:2117 -11.3 -23.6 68.3 -12.3 0 -4.3 CCAAAGGTCTCAAAGTTGAA

5539 SEQ ID N0:2118 -11.3 -19.9 59.3 -8.6 0 -5.4 TGCCAAAGGTCTCAAAGTTG

5541 SEQ TD N0:2119 -11.3 -21.8 63.8 -10.5 0 -4 AGGAGGATCCAGGGCAGCTG

5921 SEQ ID N0:2120 -11.3 -28.3 80 -14.3 -2.6 -12.5 AGTGGATCCGGTCACCACTG

5997 SEQ ID N0:2121 -11.3 -27.7 76.6 -13.3 -3 -13.5 GGCTCATAAGAGACTTTGGA

6121 SEQ ID N0:2122 -11.3 -22.3 66.5 -9.6 -1.3 -5 TGCCTCTTTGTTATAGTTAC

6245 SEQ ID N0:2123 -11.3 -22.1 67.8 -10.8 0 -3 GGTTTTGTTACACTATCATA

6379 SEQ ID N0:2124 -11.3 -20.2 63 -8.9 0 -2.7 TACTTTTGATTTTCTCTGAC

6454 SEQ ID N0:2125 -11.3 -19.4 61.4 -8.1 0 -2.4 TCTTTTTACTTTTGATTTTC

6460 SEQ ID N0:2126 -11.3 -7.8.1 58.8 -6.8 0 -2 TATCCCTATAGTCTAGGTAG

6723 SEQ ID N0:2127 -11.3 -22.7 69.1 -10 -1.3 -4.6 TCTTCAACGTAAACCTCATT

6911 SEQ ID N0:2128 -11.3 -20.6 60.7 -9.3 0 -5.3 TTCTTCAACGTAAACCTCAT

6912 SEQ ID N0:2129 -11.3 -20.6 60.7 -9.3 0 -5.3 CATTTGAGAGACAGGGTTCT

6942 SEQ ID N0:2130 -11.3 -22.6 68.2 -11.3 0 -3.6 ATTAAATATAGAGCTCTGAA

7202 SEQ ID N0:2131 -11.3 -16.3 52.7 -4 0 -10 ATTCATTCTATCACCAATAT

7631 SEQ ID N0:2132 -11.3 -19.3 59.4 -8 0 -2.4 ATTCTGCTTGACATAATGGA

7664 SEQ ID N0:2133 -11.3 -20.5 61.8 -9.2 0 -3.3 CTTCAAATTATTCTGCTTGA

7673 SEQ ID N0:2134 -11.3 -19.3 59.5 -8 0 -3.6 AAGTGAAATGCAATAAAAAA

8473 SEQ ID N0:2135 -11.3 -11.7 42.4 0 0 -5.6 ATTAATACCTTATGTATACA

8898 SEQ ID N0:2136 -11.3 -17.2 54.7 -5 -0.8 -7.9 TTTATTATGGGAGAATTATG

9062 SEQ ID N0:2137 -11.3 -16.4 53.2 -5.1 0 -3.2 ATTTATTATGGGAGAATTAT

9063 SEQ ID N0:2138 -11.3 -16.4 53.2 -5.1 0 -3.2 GACCTTTCCAGAATCCTCTC

22 SEQ ID N0:2139 -11.2 -25.9 74 -14.7 0 -3.5 ACAGGTATCTCATCCCTGTC

269 SEQ ID N0:2140 -11.2 -26.3 77.4 -12.6 -2.5 -7.5 GTCACTATTTGGCTTTGGTT

614 SEQ ID N0:2141 -11.2 -23.9 72 -12.7 0 -3.7 TTTTCTTATTGATATAGTAG

717 SEQ ID N0:2142 -11.2 -16.4 54.7 -5.2 0 -2.7 GTTTTCAGTGCTCGGAGAAC

1129 SEQ ID N0:2143 -11.2 -23.6 69.9 -11.8 -0.3 -6.4 GTGTAGCCATAGTTGGGGTT

1543 SEQ ID N0:2144 -11.2 -26.5 78.3 -13.8 -1.4 -4.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGAGGAATCCTCCAGCATCT

2552 SEQ ID N0:2145 -11.2 -25.9 74 -12.5 -2.2 -11.2 CCATAAAGAGGGTATTTAAG

2784 5EQ ID N0:2146 -11.2 -18.1 55.9 -6.9 0 -3.1 CAAGGTGAGGTTTCCTAGAG

3113 5EQ ID N0:2147 -11.2 -23.2 69.2 -11.3 -0.5 -4.4 ACAAGGTTTCCAATGACCAT

3373 SEQ ID N0:2148 -11.2 -22.7 64.9 -9.9 -1.5 -5.8 AACACTCCCGCATCTTATTT

3528 SEQ ID N0:2149 -11.2 -24.3 68.1 -13.1 0 -3.6 CATCGATTACGTATTTTTCA

3711 SEQ ID N0:2150 -11.2 -19.7 59.5 -7.6 -0.7 -6.3 ACACCCTTCAGTAAAACAAG

3971 SEQ ID N0:2151 -11.2 -20.2 59.2 -9 0 -2.9 AACAATACTGTAGCAGGTTT

4061 SEQ ID N0:2152 -11.2 -20.3 61.8 -8.2 -0.7 -5.3 GGGATAAGGCTCTTAGAGGT

4401 SEQ ID N0:2153 -11.2 -24 71.5 -12.2 -0.3 -6.1 TTTTTCCACCGAGCTTGCTT

4636 SEQ ID N0:2154 -11.2 -26.7 74.3 -15 -0.2 -6.1 CAGATTTTCTTCATATACAG

4778 SEQ ID N0:2155 -11.2 -18.4 58.5 -7.2 0 -2.6 ATTTGTTTGCTGGGCGAGGT

4995 SEQ ID N0:2156 -11.2 -26.3 75.3 -14.3 -0.6 -4.4 GGATCATGATGCTGATATCA

5055 SEQ ID N0:2157 -11.2 -21.6 64.7 -9.5 -0.6 -8.5 TTGGACATCCCAAAGATGGC

5479 SEQ ID N0:2158 -11.2 -24.1 67.4 -11.2 -1.7 -7.8 TCATCAATTCCAGCTTCCTT

5515 SEQ ID N0:2159 -11.2 -24.9 72 -13.7 0 -4.5 ATCGGGATCAAACTTTTCCC

5861 SEQ TD N0:2160 -11.2 -23.7 66.7 -10.9 -1.5 -6.5 ACTCCCATCTGTTTTTTCTG

6335 SEQ ID N0:2161 -11.2 -24.5 72.3 -13.3 0 -2.l TCCTTGTCTGGTTTTGTTAC

6388 SEQ ID N0:2162 -11.2 -23.8 72.6 -12.6 0 -3.2 GTTTGGCATGGACCTCCTCT

6552 SEQ ID N0:2163 -11.2 -28.6 80.6 -16.9 -0.1 -5.l ATAGAATGGATGCAAGGACT

6788 SEQ ID N0:2164 -11.2 -20.1 60.2 -8.9 0 -5.6 AGGCACATAACTGTTCAGAT

7103 SEQ ID N0:2165 -11.2 -21.7 65 -10 -0.2 -5.6 ACATAGACTATCAGCTTTGC

7347 SEQ ID N0:2166 -11.2 -21.8 66.2 -10.6 0 -4.5 TGTTGGAAGCTTGCAAAAGA

7487 SEQ ID N0:2167 -11.2 -20.2 60 -6.9 -2.1 -7 ATAGCCTTTAAACTATGTTT

7531 SEQ ID N0:2168 -11.2 -19.1 58.8 -7.3 -0.3 -5 ACCATTCTTTTTAGCAATAG

8416 SEQ ID N0:2169 -11.2 -19.9 60.9 -8.7 0 -4.1 TTGAAAGAAGATGAGTTTAG

8795 SEQ ID N0:2170 -11.2 -15.5 51.2 -4.3 0 -2.2 TTAATACCTTATGTATACAT

8897 SEQ ID N0:2171 -11.2 -17.2 54.7 -5 -0.8 -9.3 AGCTTTTTTTTTTTTTTTTT

114 SEQ ID N0:2172 -11.1 -18.3 59.3 -7.2 0 -4.3 CGTGTCTTCCTCTGGCAGCT

138 SEQ ID N0:2173 -11.1 -29.6 83.9 -18.5 0 -5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCATCCCTGTCAAACCTTGA

260 SEQ ID N0:2174 -11.1 -25.1 70.3 -13 -0.9 -4.3 TCCAGGTCCCTTCTGTGAAT

368 SEQ ID N0:2175 -11.1 -27.3 77.1 -15.7 -0.2 -4.2 TAGTAAAAAGGCGGAAGCTT

507 SEQ ID N0:2176 -11.1 -19.5 57.8 -6 -2.4 -7 GTCATAAATACACAGTTGGT

889 SEQ ID N0:2177 -11.1 -19.8 60.9 -8.7 0 -2.6 GGTCATAAATACACAGTTGG

890 SEQ ID N0:2178 -11.1 -19.8 60.4 -8.7 0 -2.8 GGGTTGCTCAAGGTCATAAA

901 SEQ ID N0:2179 -11.1 -22.6 66.7 -11.5 0 -3.4 TGTGTAGCCATAGTTGGGGT

1544 SEQ ID N0:2180 -ll.l -26.4 77.6 -13.8 -1.4 -4.8 TAACCCACCTACTCCACTGA

1886 SEQ ID N0:2181 -11.1 -26.6 72 -15.5 0 -1.6 CCATGTCTGTGCGGCACAAA

2305 SEQ ID N0:2182 -11.1 -26.2 71.5 -12.5 -1.4 -13.2 TACTTTTAACCATGCATCAC

2699 SEQ ID N0:2183 -11.1 -20.8 62 -9.7 0 -6.6 GAATTGCTCAGTCATGGGGT

2816 SEQ ID N0:2184 -11.1 -24.9 73.2 -13.8 0 -4.7 TTTCCAATGACCATGACCAA

3367 SEQ ID N0:2185 -11.1 -22.9 64 -11.8 0 -4.5 GTTTCCAATGACCATGACCA

3368 SEQ ID N0:2186 -11.1 -24.8 69 -13.7 0 -4.5 TTTGGAAACACTCCCGCATC

3534 SEQ ID N0:2187 -11.1 -24.7 68 -12.5 -1 -5.7 CTCTAAGATAATTAAGCTCT

3648 SEQ ID N0:2188 -11.1 -17.9 56.6 -6.8 0 -5.1 GGTGAGGCTGGGGTTGTTTA

3752 SEQ ID N0:2189 -11.1 -26.9 79.2 -15.8 0 -3.7 CAACAGCAATTGGCACTGTG

3774 SEQ ID N0:2190 -11.1 -22.8 65.5 -10.1 -1.6 -9.6 TCTCGGGGTAGAACAACATC

3904 SEQ ID N0:2191 -11.1 -22.4 65.5 -11.3 0 -3.7 CTTCTCGGGGTAGAACAACA

3906 SEQ ID N0:2192 -11.1 -23 66.2 -11.3 -0.3 -3.8 TACTGTAGCAGGTTTTTCGA

4056 SEQ ID N0:2193 -11.1 -22.8 68.4 -10.8 -0.7 -5.5 AGCACATTCATGATAGAGGG

4468 SEQ ID N0:2194 -11.1 -21.9 65.7 -10.8 0 -6.4 TATGGGTTTCTGAGGTTTCT

4976 SEQ ID N0:2195 -11.1 -23.3 71.5 -12.2 0 -2.8 GGCGAGGTATGGGTTTCTGA

4983 SEQ ID N0:2196 -11.1 -26.2 75.5 -15.1 0 -4 TTTGCCCTGGTCATCCGTTT

5108 SEQ ID N0:2197 -11.1 -29 79.5 -16.7 -1.1 -4.4 GCTTCAGCACAAATTCTCCA

5181 SEQ ID N0:2198 -11.1 -24.4 70 -13.3 0 -5.g CATCCCAGCCAGCAGAGGTT

5589 SEQ ID N0:2199 -11.1 -29.9 82.1 -18.8 0 -4.6 GCTGGACTTTGTTGGGTTTT

5g52 SEQ ID N0:2200 -11.1 -25 74.2 -13.9 0 -28 TCCTCCCTTTAATTGCCTCT

6258 SEQ ID N0:2201 -ll.l -27.9 77.1 -16.8 0 -3 AAGTCAATCCTCCCTTTAAT

6265 SEQ ID N0:2202 -ll.l -22.7 65.5 -11.6 0 -2.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTTATAGGTAAGTCAATCCT

6274 SEQ ID N0:2203 -11.1 -19.9 61.5 -8.8 0 -3.8 CCTCTTTTCCTTTGCTTTCT

6435 5EQ ID N0:2204 -11.1 -26.2 76.6 -15.1 0 -3.6 TAGTTACAATGTTCACTTTG

6749 SEQ ID N0:2205 -11.1 -18.6 58.9 -7.5 0 -5.6 AAAACACCTTTGTCTGATCA

6960 SEQ ID N0:2206 -11.1 -20.3 60.5 -8.5 -0.5 -6.8 ACTTTACAGGCACATAACTG

7110 SEQ ID N0:2207 -11.1 -20.6 61.8 -8.8 -0.5 -4 AAGCAATACTTTAGATATAT

7272 SEQ ID N0:2208 -11.1 -15.8 51.7 -4.7 0 -4.1 TTGATTTAATAGAAGTTGTT

7446 SEQ ID N0:2209 -11.1 -16.1 53 -5 0 -2.5 AGCATAGGCAATATTAACAT

7560 SEQ ID N0:2210 -11.1 -18.9 57.8 -6.2 -1.6 -6.7 AGGTTGAATTCATTCTATCA

7638 SEQ ID N0:2211 -11.1 -19.8 61.8 -8.2 0.3 -8.4 CCATATGATACTCATGTTGA

7728 SEQ ID N0:2212 -11.1 -20.8 62.5 -8.8 -0.8 -6 TATCCAGTGTGTTTCCTTGA

7762 SEQ TD N0:2213 -11.1 -24.6 73.1 -13.5 0 -3.7 TTTAGGAAATTATCCATTGA

7998 SEQ ID N0:2214 -11.1 -17.9 55.8 -4.6 -2.2 -7.2 TATCTAACTTTTATTTTAGG

8012 SEQ ID N0:2215 -11.1 -16.3 53.8 -5.2 0 -3.2 TGAGTGTTTGACCAATGTAT

8086 SEQ ID N0:2216 -11.1 -20.9 63.1 -9.8 0 -3.4 CATGTTATATTTGTAATAGC

8239 SEQ ID N0:2217 -11.1 -17.3 55.7 -6.2 0 -3.5 AACAAAAATTTATATCATGT

8311 SEQ ID N0:2218 -ll.l -13.3 45.9 -2.2 0 -4.7 ATGAAATTTATTCTTTTGGC

8454 SEQ ID N0:2219 -11.1 -17.8 56.2 -5.6 -1 -5.2 GAGTTTATATAATGCATTTA

8875 SEQ ID N0:2220 -ll.l -16.8 54.4 -5 0 -8.8 AGAGTTTGGTTTTGACCTCA

8974 SEQ ID N0:2221 -ll.l -23.5 70.6 -10.1 -2.3 -6.3 CTTTCTCTGTGGATAAGAAA

49 SEQ ID N0:2222 -11 -18.9 58.6 -6.8 -1 -4.5 TTTTTTTTTTTTTTTTTTTT

111 SEQ ID N0:2223 -11 -15.9 53.7 -4.9 0 0 ATTCTTTGCTCCTTTCCCAG

314 SEQ ID N0:2224 -11 -26.9 76.8 -15.9 0 -3.6 TGTAGCTTCTTGCATACGAA

426 SEQ TD N0:2225 -11 -22.2 65.4 -9.4 -1.8 -5.2 GTTGGTCAAAATAGTGCACA

875 SEQ ID N0:2226 -11 -21 62.9 -9 0 -9.9 AGCAAAACCCTCTTGCCAAG

990 SEQ ID N0:2227 -11 -24.7 67.4 -11.7 -2 -6.2 TTAACAAATGTCCCATTTGA

1372 SEQ ID N0:2228 -11 -19.7 58.4 -4.9 -3.8 -10.4 CCTAACCCACCTACTCCACT

lggg SEQ ID N0:2229 -11 -28.9 75.9 -17.9 0 -0.3 TTGGAACTCAACTTTGATGC

1933 SEQ ID N0:2230 -1l -20.4 61.3 -9.4 0 -5 AAGGTGCTCTCTCTGTCTTC

1988 SEQ ID N0:2231 -11 -25.3 77.9 -14.3 0 -2.9 kcal/

mol kcal/moldeg koal/molkcal/molkcal/mol C

Intra- Tnter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGGGGAAAACAGGGAGCCAC

2159 SEQ ID N0:2232 -11 -24.7 68.2 -13.1 -0.3 -4.3 TCGCTCTCCATGTCTGTGCG

2312 SEQ ID N0:2233 -11 -28.6 79.6 -16.3 -l.2 -6.7 TGGAAGCCCTGGCACCATCC

2369 SEQ ID N0:2234 -11 -30.8 80.6 -18.2 -1.4 -10.4 CAGAGGAATCCTCCAGCATC

2553 SEQ ID N0:2235 -11 -25.7 73.1 -12.5 -2.2 -11.2 AAATGAAGACGATGATGGCC

3138 SEQ ID N0:2236 -11 -20.3 58.6 -9.3 0 -6.2 AACAGCAATTGGCACTGTGA

3773 SEQ ID N0:2237 -11 -22.7 65.7 -10.1 -1.6 -9.6 TTTCAGCTTGTTCACCTTCT

3921 SEQ ID N0:2238 -11 -25 74.8 -14 0 -4.5 TCCCTTTGCCTTCTTCTGTA

4020 SEQ ID N0:2239 -11 -27.9 79.9 -16.9 0 -3 AGAAACATCAACGATCAAGA

4301 SEQ ID N0:2240 -11 -17.1 52.9 -6.1 0 -4.9 ATGTCAAACATGTTACCCGT

4576 SEQ ID N0:2241 -11 -23 65.2 -11.1 -0.6 -9 AGAATACTTTTCTATCATCT

5294 SEQ ID N0:2242 -11 -18.4 58.7 -6.5 -0.8 -3.4 CTCCCTTAACTGAGCTGCCA

5670 SEQ ID N0:2243 -11 -28.2 76.8 -17.2 0 -5.6 AGGTAAGTCAATCCTCCCTT

6269 SEQ ID N0:2244 -11 -25.7 73.4 -14.7 0 -4.7 TTTCTGGAGTGGAGTTCCCA

6321 SEQ ID N0:2245 -11 -26.7 77.5 -14.6 -1 -8.7 GGAGGGGTGGTAGAGGAACT

6352 SEQ ID N0:2246 -11 -25.5 74.3 -13.8 -0.5 -3.1 TTCTTTTTACTTTTGATTTT

6461 SEQ ID N0:2247 -11 -17.8 57.7 -6.8 0 -2.2 CAACGTTGATACCCTGCTTC

6648 SEQ ID N0:2248 -11 -24.9 69.3 -13 0 -9.8 TACAATGTTCACTTTGCACA

6745 SEQ ID N0:2249 -11 -21 63 -10 0 -5.4 ATTGGGGACCATGGAAATGC

6853 SEQ ID N0:2250 -11 -23.4 65.9 -10 -2.4 -8.8 CAAAACACCTTTGTCTGATC

6961 SEQ ID NO:2251 -11 -20.3 60.5 -8.5 -0.6 -5.1 GAAAGCATTTGACCTAAATT

7219 SEQ ID N0:2252 -11 -18 54.9 -6.3 -0.4 -4 TTACTTTTTGGAAAGCATTT

7229 SEQ ID N0:2253 -11 -18.4 57.3 -5.8 -1.6 -5.5 TTATTAGATTACTTTTTGGA

7237 SEQ ID N0:2254 -11 -17.3 56 -6.3 0 -2.4 GGATTTATTAGATTACTTTT

7241 SEQ ID N0:2255 -11 -17.3 56.1 -6.3 0 -2.4 GATACAGGATACATCCCTGT

7504 SEQ ID N0:2256 -11 -24.1 69.5 -10.3 -2.8 -8.2 AATATTAACATGCATTAGTG

7551 SEQ ID N0:2257 -11 -16.5 53 -5.5 0 -6.7 GCAATATTAACATGCATTAG

7553 SEQ ID N0:2258 -11 -17.8 55.4 -5.5 -1.2 -6.7 ATAGGCAATATTAACATGCA

7557 SEQ ID N0:2259 -11 -18.9 57.5 -5.9 -2 -6.8 GACATTCTTTAACCAAGACT

7609 SEQ ID N0:2260 -11 -20 60.1 -8.2 -0.6 -3.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTTTAGACATATTTTTAGCA

7814 SEQ TD N0:2261 -11 -17.8 57.1 -6.8 0 -4.l TAGTTACAATTTTTTTTCTT

70 SEQ ID N0:2262 -10.9 -17 55.7 -6.1 0 -2.6 TTAGTTACAATTTTTTTTCT

71 SEQ ID N0:2263 -10.9 -17 55.7 -6.1 0 -2.6 ATTCTTACAATATCCCTAGA

338 SEQ ID NO:2264 -10.9 -20.7 62.2 -9.8 0 -3.8 AGGCGGAAGCTTTCAGGTCC

499 SEQ ID N0:2265 -10.9 -27.7 77.9 -14.4 -2.4 -7.6 TTTGTTCTCATCATCATTAT

596 SEQ ID N0:2266 -10.9 -19.9 62.9 -9 0 -1.9 TCTTGATAGCAATTTTCCTA

822 SEQ ID N0:2267 -10.9 -20.8 63.2 -9.3 -0.3 -0.5 TGGTTTCAAAAGCAGAATCG

1320 SEQ ID N0:2268 -10.9 -19.2 57.6 -8.3 0.4 -0.6 TGAGCCATTTCCACAGAGTA

1475 SEQ ID N0:2269 -10.9 -24.8 71.7 -13.9 0 -3.2 TTCGACCAGCCTTCACACAG

1524 SEQ ID N0:2270 -10.9 -26.5 73.3 -15.6 0 -4.6 AAGGTGGCCTGATTCTGCTC

1756 SEQ ID N0:2271 -10.9 -26.7 76.8 -15.1 -0.4 -6.5 GCTTTTTAAGCTGTTCGAGC

1815 SEQ ID N0:2272 -10.9 -23.7 70 -10.9 -1.9 -8.7 TTGGGGAAAACAGGGAGCCA

2160 SEQ ID N0:2273 -10.9 -24.6 68 -13.1 -0.3 -4.7 TTGCGTCTTGGGGAAAACAG

2167 SEQ TD N0:2274 -10.9 -22.3 63.9 -11.4 0 -5.2 TTCGCCGTCTTCAAATGTGC

2267 SEQ ID N0:2275 -10.9 -25.2 70.2 -14.3 0 -2.8 GGGAAGTTGTCCAGTAGGTG

2465 SEQ ID N0:2276 -10.9 -25.2 74.8 -12.3 -2 -5.6 CAGTAAAGACCAGGTTTCCT

2853 SEQ ID N0:2277 -10.9 -23.6 68.3 -12.7 0 -5.9 CAGACCAAGCTCCATTAAAC

2975 SEQ ID N0:2278 -10.9 -21.9 62.8 -11 0 -5 GACAGACCAAGCTCCATTAA

2977 SEQ ID N0:2279 -10.9 -23.2 66 -12.3 0 -5 TGCTTCCTTCAGATGAGCTG

3879 SEQ ID N0:2280 -10.9 -25.2 73.7 -12.9 -1.3 -5.3 CTTCTGTACTTACTTGACAG

4008 SEQ ID N0:2281 -10.9 -20.8 64.3 -8.8 -1 -4.6 TCTTCTGTACTTACTTGACA

4009 SEQ ID N0:2282 -10.9 -21.2 65.6 -10.3 0 -4.8 CAGGTTTTTCGAAGATTCCA

4048 SEQ ID N0:2283 -10.9 -22.4 65.5 -10.8 -0.4 -7.4 CAATGAAAGTCTCAAACCAG

4089 SEQ ID N0:2284 -10.9 -18.3 55.5 -6.8 -0.3 -3.4 CCCTCATGCCTTCAAACCGG

4419 SEQ ID N0:2285 -10.9 -28.7 74.2 -17.8 0 -6.6 CATAAAGATGTCTTGACCTC

4907 SEQ ID N0:2286 -10.9 -20.3 61.4 -8.6 -0.6 -6.8 AACTAGGGTCATGTATTTGC

5123 SEQ ID N0:2287 -10.9 -21.7 66 -10.8 0 -4.7 TCATCAAAGCAAAGAGCAGC

5400 SEQ ID N0:2288 -10.9 -20.8 61.7 -8.3 -1.6 -5.5 CACCACTGACCATGGGCAGA

5985 SEQ ID N0:2289 -10.9 -27.7 75 -15.7 -1 -8.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGGAAGGAGGAGGGGTGGTA

6360 SEQ ID N0:2290 -10.9 -25.6 74.7 -14.7 0 -0.6 TAGGAAGGAGGAGGGGTGGT

6361 SEQ ID N0:2291 -10.9 -25.6 74.7 -14.7 0 -0.2 AACTTTTCCTTGTCTGGTTT

6394 SEQ ID N0:2292 -10.9 -23.2 69.7 -12.3 0 -3.2 GGCACTGACTATGAGTATTT

6585 SEQ ID N0:2293 -10.9 -21.9 66.1 -10.5 -0.1 -4.1 TGTTCACTTTGCACAACTAT

6740 SEQ ID N0:2294 -10.9 -21.2 63.8 -10.3 0 -5 ATGTTCACTTTGCACAACTA

6741 SEQ ID N0:2295 -10.9 -21.2 63.8 -10.3 0 -5 GTTACAATGTTCACTTTGCA

6747 SEQ ID N0:2296 -10.9 -21.4 64.8 -10.5 0 -5.4 ACTATGAATTGGGGACCATG

6860 SEQ ID N0:2297 -10.9 -21.9 63.4 -8.6 -2.4 -,7.2 GGCACATAACTGTTCAGATA

7102 SEQ ID N0:2298 -10.9 -21.4 64.2 -10 -0.2 -5.6 TTTTTGGAAAGCATTTGACC

7225 SEQ ID N0:2299 -10.9 -20.3 60.6 -8.7 -0.4 -4.1 TGGATTTATTAGATTACTTT

7242 SEQ TD N0:2300 -10.9 -17.2 55.7 -6.3 0 -2.4 ATTATTCTGCTTGACATAAT

7667 SEQ ID N0:2301 -10.9 -18.5 57.8 -7.1 -0.2 -4.2 TCCTCTCTGCTTATGCCTCC

9 SEQ ID N0:2302 -10.8 -29.5 83.4 -17.8 -0.8 -4.6 TTTTTTTTTTTTTTTTTTTT

110 SEQ ID N0:2303 -10.8 -15.9 53.7 -5.1 0 0 TTGACAGGTATCTCATCCCT

272 SEQ ID N0:2304 -10.8 -25.4 73.8 -14.6 0 -3.1 GATTCTTTGCTCCTTTCCCA

315 SEQ ID N0:2305 -10.8 -27.5 77.9 -16.7 0 -3.6 CCAGGTCCCTTCTGTGAATT

367 SEQ TD N0:2306 -10.8 -27 75.8 -15.7 -0.2 -4.2 TCTCATCATCATTATCTTGT

591 SEQ ID N0:2307 -10.8 -21 65.8 -10.2 0 -1.9 TATTTGGCTTTGGTTTGTTC

609 SEQ ID N0:2308 -10.8 -22.3 68.7 -11.5 0 -3.7 ATATAGTAGGGATCCAGGTC

706 SEQ ID N0:2309 -10.8 -23.2 70.4 -11.6 -0.3 -8.9 TTTGTCCAGTCAGGAGGGTT

916 SEQ ID N0:2310 -10.8 -26.3 78.4 -14.1 , -1.3 -5.3 TCGCTTGGGGGCCACTGCAA

1303 SEQ ID N0:2311 -10.8 -30.2 80 -17.5 -1.9 -6.9 CCTGAGCTTCTTCCTGTTGC

1833 SEQ ID N0:2312 -10.8 -28.1 80.7 -17.3 0 -5.2 GCTTCTGAAGAACTTTCCAA

1915 SEQ ID N0:2313 -10.8 -21.6 63.6 -10.1 -0.4 -8.3 CTCCATTCTTTAGCACCTTT

1951 SEQ ID N0:2314 -10.8 -25.1 72.7 -14.3 0 -4.1 TTCAAGGTGCTCTCTCTGTC

1991 SEQ ID N0:2315 -10.8 -25.1 76.9 -14.3 0 -3.6 TCTCTCCTTTGTTGTTTCCT

2010 SEQ ID N0:2316 -10.8 -26.1 78.1 -15.3 0 0 CTCTCTCCTTTGTTGTTTCC

2011 SEQ ID N0:2317 -10.8 -26.1 78.1 -15.3 0 0 TTGCCCGACCTCTGAAACTG

2205 SEQ ID N0:2318 -10.8 -26 69.6 -15.2 0 -3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CTCCATGTCTGTGCGGCACA

2307 SEQ ID N0:2319 -10.8 -28.9 79.7 -15.6 -1.3 -13 TTCCCATTTGCTGGAAGCCC

2380 SEQ ID N0:2320 -10.8 -29 77.8 -16 -2.2 -6.6 CATTGCAATCCACAGTGCTG

2406 SEQ ID N0:2321 -10.8 -24.5 69.7 -12.1 -1.5 -9 GGTTTCCTAGAGCCCCCACA

3105 SEQ ID N0:2322 -10.8 -31.6 84.2 -20.8 0 -3.8 ATCATCAGTAGCAGCAAGGT

3443 SEQ ID N0:2323 -10.8 -23.8 71.4 -13 0 -5.4 GGCTTTTTGGAAACACTCCC

3539 SEQ ID N0:2324 -10.8 -25.1 70.2 -13.2 -1 -5.8 ACTGTGACGGTGAGGCTGGG

3760 SEQ ID N0:2325 -10.8 -27.2 76.4 -16.4 0 -3.7 ATTTCTCTTTGCTTTCTTCT

3849 SEQ ID N0:2326 -10.8 -22.6 70.5 -11.8 0 -3.6 CATTTCCAGAATGAATATAT

4217 SEQ ID N0:2327 -10.8 -17.2 53.9 -5.4 -0.9 -5.7 ACATCAACGATCAAGAAATC

4297 SEQ ID N0:2328 -10.8 -16.9' 52.7 -6.1 0 -4.9 ACGTTTTTCCACCGAGCTTG

4639 SEQ ID N0:2329 -10.8 -26.1 71.7 -15.3 0 -5.3 ATTCTCCAGTGAACAGAACA

5169 SEQ ID N0:2330 -10.8 -21.3 63.3 -10 -0.2 -4.8 GCAGAGGTTGTAATTTGGAA

5578 SEQ ID N0:2331 -10.8 -21.1 63.6 -10.3 0 -5.7 GATGACCGCGATGTACATGT

5762 5EQ ID N0:2332 -10.8 -24.4 67.7 -12.9 0 -8.9 GGGCATCCATCTCTCCACTC

6060 5EQ ID N0:2333 -10.8 -29.2 82.9 -17.8 -0.3 -4 ATGCCATAAACCTGTCTTCC

6093 SEQ ID N0:2334 -10.8 -24.9 69.9 -14.1 0 -3 TTTTCTGGAGTGGAGTTCCC

6322 SEQ ID N0:2335 -10.8 -26.1 76.8 -14.6 -0.5 -4.5 AGTTACAATGTTCACTTTGC

6748 SEQ ID N0:2336 -10.8 -20.7 63.8 -9.9 0 -5.3 AAGTTAAAAATAGAATGGAT

6797 SEQ ID N0:2337 -10.8 -12.9 45.1 -2.1 0 -2.8 TAAACTATGAATTGGGGACC

6863 SEQ ID N0:2338 -10.8 -19.5 58 -7.5 -1.1 -5.7 GCATTATGAATAAACTATGA

6873 SEQ ID N0:2339 -10.8 -15.9 51.2 -5.1 0 -3.5 ACAATTCTTTTTCTGGTTTT

7004 SEQ ID N0:2340 -10.8 -20 62.5 -9.2 0 -2.5 TTGGAAGCTTGCAAAAGACA

7485 SEQ ID N0:2341 -10.8 -19.9 59 -7 -2.1 -7 CCATAGTTGGTAGAAAATGA

8114 SEQ ID N0:2342 -10.8 -18.9 57.5 -7.3 -0.6 -3.5 CCATGTTATATTTGTAATAG

8240 SEQ ID N0:2343 -10.8 -17.5 55.6 -6.7 0 -4.3 ATTCTTTTTAGCAATAGCAA

8413 SEQ ID N0:2344 -10.8 -18.8 58.6 -6.3 -1.7 -4.5 CATTCTTTTTAGCAATAGCA

8414 SEQ ID N0:2345 -10.8 -20.2 61.9 -7.7 -1.7 -4.5 AATTTATTCTTTTGGCTCAA

8450 SEQ ID N0:2346 -10.8 -19.2 59.6 -8.4 0 -4.6 ACCCCAGAGCCCAATAAGTG

8488 SEQ ID N0:2347 -10.8 -27.5 72.9 -16.7 0 -3.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTATATATATTTTAAACTGC

8533 SEQ ID N0:2348 -10.8 -14.4 48.9 -3.6 0 -5.2 TTGTAGGTGGTTACTACTAT

8736 SEQ ID N0:2349 -10.8 -21.2 65.9 -8.8 -1.6 -5.2 ATGAGTTTAGATGCTTATAG

8785 SEQ ID N0:2350 -10.8 -18.5 59.1 -7.7 0 -3.6 TACATAGGAGTTTATATAAT

8882 SEQ ID N0:2351 -10.8 -15.9 52.5 -5.1 0 -5.5 ATACATAGGAGTTTATATAA

8883 SEQ TD N0:2352 -10.8 -15.9 52.5 -5.1 0 -5.2 AATTTTTTTTCTTTCTTTCT

63 SEQ ID N0:2353 -10.7 -18.3 59.3 -7.6 0 -2.3 GCTGTTCAGCTTTTTTTTTT

121 SEQ ID N0:2354 -10.7 -22.7 69.9 -10.9 -0.9 -9.3 GGGTATAACGTGTCTTCCTC

146 SEQ ID N0:2355 -10.7 -24.8 72.6 -14.1 0 -5.3 CCCTGTCAAACCTTGATGTG

256 SEQ ID N0:2356 -10.7 -24.8 69 -13.3 -0.6 -4.5 CTTTGCTCCTTTCCCAGTAA

311 SEQ ID N0:2357 -10.7 -26.6 75.1 -15.9 0 -3.6 TTTCCGGAAAAGCTCCAGGT

381 SEQ ID N0:2358 -10.7 -25.3 69.7 -12.5 -1.9 -11.8 CAGTGCCTGTGCCATCTTTT

467 SEQ ID N0:2359 -10.7 -27.9 79.1 -16.4 -0.6 -5.2 TATTGATATAGTAGGGATCC

711 SEQ ID N0:2360 -10.7 -20.1 62.2 -8.9 0 -7.7 TTATTGATATAGTAGGGATC

712 SEQ ID N0:2361 -10.7 -18.2 58.5 -7.5 0 -4.1 AGAGCAAACACGCTCAGACA

1240 SEQ ID N0:2362 -10.7 -22.9 65.3 -8.9 -3.3 -8.5 AGCAGAATCGCTTGGGGGCC

1310 SEQ ID N0:2363 -10.7 -29 78.7 -17.6 -0.5 -7.1 CCTTCACACAGATGTATCCT

1515 SEQ ID N0:2364 -10.7 -24.7 71.2 -13.4 -0.3 -3.7 TGTAGCCATAGTTGGGGTTT

1542 SEQ TD N0:2365 -10.7 -25.4 74.9 -13.8 -0.7 -4.8 CAAGGTGGCCTGATTCTGCT

1757 SEQ ID N0:2366 -10.7 -27 76.2 -15.6 -0.4 -7.2 GTCACTGGTCAGTCTGTTTC

2099 SEQ ID N0:2367 -10.7 -25.2 78.8 -14.5 0 -6.6 AGCCACGGATACTCAAGAGA

2145 SEQ ID N0:2368 -10.7 -23.7 67.3 -13 0 -5.4 GACAATAATTCCATCAAAGA

2948 SEQ ID N0:2369 -10.7 -17.3 53.6 -6.6 0 -2.9 AACACAATCAGGAAGGAGTG

3262 SEQ ID N0:2370 -10.7 -19.6 59.2 -8.4 -0.2 -4.7 AAGGTTTCCAATGACCATGA

3371 SEQ ID N0:2371 -10.7 -22.4 64.4 -10.1 -1.5 -5.8 AACCACAAGGTTTCCAATGA

3377 SEQ ID N0:2372 -10.7 -22 62.9 -9.3 -2 -6.7 GGCCAATGCACCACTACTGA

4124 SEQ ID N0:2373 -10.7 -26.9 73.1 -14.7 -1.4 -7 AGCATATTCTAGCATGGTTT

4181 SEQ ID N0:2374 -10.7 -22.4 68.1 -10.7 -0.9 -5 GAAATCTAGCCAGCACCAGG

4283 SEQ ID N0:2375 -10.7 -25.3 70.6 -14.6 0 -4.6 TGAGGTTTCTTGGATCCAAG

4966 SEQ ID N0:2376 -10.7 -22.8 67.7 -8.7 -1 -14.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CACAGAATACTTTTCTATCA

5297 SEQ ID N0:2377 -10.7 -18.7 58.5 -7 -0.9 -4.6 GCAGGAGGCCGATGTTAAAC

5436 SEQ ID NO:2378 -10.7 -24.5 68.4 -13.2 -0.3 -7.7 TCAATTCCAGCTTCCTTTTT

5512 SEQ ID N0:2379 -10.7 -24.1 70.4 -13.4 0 -4.5 TCTCCCTTAACTGAGCTGCC

5671 SEQ ID N0:2380 -10.7 -27.9 77.5 -17.2 0 -5.6 AAGGGCATCCATCTCTCCAC

6062 SEQ TD N0:2381 -10.7 -27.2 76.7 -15.3 -1.1 -4 AATAGGCTCATAAGAGACTT

6125 SEQ TD NO:2382 -10.7 -19.4 59.8 -7.3 -1.3 -5.2 TCTGGAGTGGAGTTCCCATT

6319 SEQ ID N0:2383 -10.7 -26.6 77.1 -14.6 -1.2 -6.7 TTCTGGAGTGGAGTTCCCAT

6320 SEQ ID NO:2384 -10.7 -26.6 77.1 -14.6 -1.2 -8.7 TTCTGGTTTGTCTTTCTCAA

6413 5EQ TD N0:2385 -10.7 -22.4 69.4 -11.7 0 -2.7 CTTTTTACTTTTGATTTTCT

6459 SEQ ID N0:2386 -10.7 -18.6 59.5 -7.9 0 -2.2 AGGCACTGACTATGAGTATT

6586 SEQ ID N0:2387 -10.7 -21.8 66 -10.5 -0.3 -4.1 TGCTTCACAGAGTTGCAGTG

6634 SEQ ID N0:2388 -10.7 -24.4 73.1 -13 -0.4 -'S.3 CAGGCACATAACTGTTCAGA

7104 SEQ ID N0:2389 -10.7 -22.4 66.3 -11.7 0 -5.6 AAATAATCCTTTAAATATTA

7136 SEQ ID N0:2390 -10.7 -12.9 45.2 -2.2 0 -5 TAAATATAGAGCTCTGAAAC

7200 SEQ ID N0:2391 -10.7 -15.7 51.2 -4 0 -10 TATTTAATTGACATAGACTA

7357 SEQ ID N0:2392 -10.7 -15.6 51.5 -4.9 0 -2.7 TAGTGATAGCCTTTAAACTA

7536 SEQ ID N0:2393 -10.7 -19.2 59.1 -8.5 0 -4.2 TTAACCAAGACTTGTGAAGA

7601 SEQ ID N0:2394 -10.7 -18.8 57.2 -7.6 -0.2 -5.4 ATCCAGTGTGTTTCCTTGAA

7761 SEQ TD N0:2395 -10.7 -24.2 71.2 -13.5 0 -3.7 GTCAGTAGGCAGTATCCAGT

7774 SEQ ID N0:2396 -10.7 -26.1 79.1 -14.9 -0.2 -4 TGTTCATGGCTAAAAAGAAC

8265 SEQ ID N0:2397 -10.7 -17.3 54 -5.6 -0.9 -5.3 AAAAATTTATATCATGTTAG

8308 SEQ ID N0:2398 -10.7 -12.9 45.5 -2.2 0 -5.2 GAAATGCAATAAAAAATGAA

8469 SEQ TD NO:2399 -10.7 -11.1 41.3 0 0 -5.6 AACCCCAGAGCCCAATAAGT

8489 SEQ ID N0:2400 -10.7 -26.8 70.9 -16.1 0 -3.2 CCTGAAAGTTGGAACATGTG

8706 SEQ ID N0:2401 -10.7 -20.6 60.6 -9.1 -0.6 -7 ATGGTTAGGGTATAACGTGT

153 SEQ ID N0:2402 -10.6 -21.9 65.5 -9.3 -2 -5.3 TTCTTTGCTCCTTTCCCAGT

313 SEQ ID N0:2403 -10.6 -28.1 80.5 -17.5 0 -3.6 AAGCTCCAGGTCCCTTCTGT

372 SEQ ID NO:2404 -10.6 -29.4 82.9 -18.3 -0.2 -6.6 GGCGGAAGCTTTCAGGTCCT

4gg SEQ TD N0:2405 -10.6 -28.6 79.6 -15.6 -2.4 -7.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GATCCAGGTCCTCCAGGGGC

696 SEQ ID N0:2406 -10.6 -31.9 88.2 -20 -1.2 -8.4 GGTCAAAATAGTGCACATGA

872 SEQ ID N0:2407 -10.6 -20.3 60.8 -8.7 0 -9.9 ACATAAAAGTGACTGTCATC

1432 SEQ ID N0:2408 -10.6 -18.1 56.8 -6.8 -0.1 -8.8 TCGCCGTCTTCAAATGTGCT

2266 SEQ ID N0:2409 -10.6 -26 71.7 -15.4 0 -3.6 ACATACTGGCCTGACTAACG

2343 SEQ ID N0:2410 -10.6 -23.3 65.5 -12.7 0 -7.2 CTCTGGGGGAAGTTGTCCAG

2471 SEQ ID N0:2411 -10.6 -26.5 76.4 -13.9 -2 -7.8 AGAACACATTGGCAAATCTA

2664 SEQ ID N0:2412 -10.6 -19.1 57.8 -8.5 0 -4.4 CCAATGATCTTAATTAGCAT

3079 SEQ ID N0:2413 -10.6 -19.3 58.4 -8.7 0 -4.2 TCCAATGACCATGACCAACA

3365 SEQ ID N0:2414 -10.6 -23.6 64.9 -13 0 -4.5 CAGCAAGGTTGTCTGAGCTA

3432 SEQ ID N0:2415 -10.6 -24.5 72.7 -12.8 -1 -7.1 ACTCCCGCATCTTATTTTTC

3525 SEQ ID N0:2416 -10.6 -24.7 70.9 -14.1 0 -3.6 GGCTTTCTAAAAAAGGCTTT

3553 SEQ ID N0:2417 -10.6 -19.7 59.1 -7.6 -1.4 -6.2 CCTTCATGGATTTCTATAAC

3577 SEQ ID N0:2418 -10.6 -20.3 61.6 -9.7 0 -5 TTCCAGTATTATTGGACATG

3615 SEQ ID N0:2419 -10.6 -20.7 62.8 -8.6 -1.4 -7.5 TCTCTAAGATAATTAAGCTC

3649 SEQ ID N0:2420 -10.6 -17.4 55.9 -6.8 0 -5.1 TTGATAGTCTTTCGCTGTTC

4162 SEQ ID N0:2421 -10.6 -22.7 69.4 -12.1 ~ 0 -3.4 TTGTCAGCATATTCTAGCAT

4186 SEQ ID N0:2422 -10.6 -22.2 67.8 -10.6 -0.9 -4.1 AGCCAGCACCAGGCATTAGT

4276 SEQ ID N0:2423 -10.6 -28.6 79.9 -15.3 -2.7 -7.4 AACGATCAAGAAATCTAGCC

4292 SEQ ID N0:2424 -10.6 -19.3 57.3 -8.7 0 -4.9 CAACCACCCTCATGCCTTCA

4425 SEQ ID N0:2425 -10.6 -29 76.7 -18.4 0 -4.4 CCGTTGTCATGTTAACACAG

4560 SEQ ID N0:2426 -10.6 -22.5 65.4 -11.1 -0.5 -8.7 TTTCTTCATATACAGGCTGA

4773 SEQ ID N0:2427 -10.6 -21.5 65.6 -10.9 0 -4.l TCTGTTCCTCTGTCATAAAG

4920 SEQ ID N0:2428 -10.6 -21.8 66.3 -11.2 0 -1.8 AATTTGTTTGCTGGGCGAGG

4996 SEQ ID N0:2429 -10.6 -24.4 69.6 -13 -0.6 -4.4 GTGACCATGTTGAGGCAGAT

5077 SEQ ID N0:2430 -10.6 -24.9 72.1 -13.8 -0.1 -4 TTCTCCAGTGAACAGAACAA

5168 SEQ ID N0:2431 -10.6 -20.6 61.3 -10 0 -3.9 AGAGCAGGAGGCCGATGTTA

5439 SEQ ID N0:2432 -10.6 -26.3 74.2 -14.8 -0.8 -7.7 GATCATGCTGTTGCCAAAGG

5552 SEQ ID N0:2433 -10.6 -24 68.5 -12.5 -0.8 -5.9 ACAGATGGGTCCCCACGGTC

5689 SEQ ID N0:2434 -10.6 -30 80.9 -18.7 -0.3 -8.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GGATGACCGCGATGTACATG

5763 SEQ ID N0:2435 -10.6 -24.4 67.1 -12.9 -0.5 -9.4 TCTGCACTTTCTTCAGTAGC

5800 SEQ ID N0:2436 -10.6 -24.3 74.4 -13.1 -0.3 -4.8 CATGGGCAGATCCATGGCAA

5975 SEQ ID N0:2437 -10.6 -26.3 72.6 -12.9 -2.8 -9.7 TTTGATGCCATAAACCTGTC

6097 SEQ TD N0:2438 -10.6 -22.3 64.5 -11.7 0 -3 CTTTAATTGCCTCTTTGTTA

6252 SEQ ID N0:2439 -10.6 -21.4 64.7 -10.8 0 -3 GGAAGGAGGAGGGGTGGTAG

6359 SEQ ID N0:2440 -10.6 -25.6 74.7 -15 0 -0.7 TTCCTTGTCTGGTTTTGTTA

6389 SEQ ID N0:2441 -10.6 -23.7 72.4 -13.1 0 -3.2 CTCCTCTTGAAGTCCAGTTG

6539 SEQ ID N0:2442 -10.6 -25.1 73.6 -14.5 0 -3 TAGCCATTGGGTTTCTCCTC

6706 SEQ ID N0:2443 -10.6 -27.1 78.8 -15.6 -0.8 -6.3 ACAATGTTCACTTTGCACAA

6744 SEQ ID N0:2444 -10.6 -20.6 61.5 -10 0 -5.4 TTAAATATAGAGCTCTGAAA

7201 SEQ ID No:2445 -10.6 -15.6 51 -4 0 -10 CTATATATATATTTGCCCAA

7419 SEQ ID N0:2446 -10.6 -19.4 58.4 -8.8 0 -6.7 AATAGAAGTTGTTTATCAGG

7439 SEQ ID N0:2447 -10.6 -17.6 56.5 -7 0 -2.9 AATGATACAGGATACATCCC

7507 SEQ ID N0:2448 -10.6 -21.3 62.3 -9.7 -0.9 -6.9 GCATTAGTGATAGCCTTTAA

7540 SEQ ID N0:2449 -10.6 -21.7 64.9 -11.1 0 -3.5 AACCCATTATCTAACTTTTA

8019 SEQ ID N0:2450 -10.6 -19.4 58.6 -8.8 0 -1.6 CAACCATAGTTGGTAGAAAA

8117 SEQ ID N0:2451 -10.6 -18.5 56.2 -5.5 -2.4 -6.3 AACAAAGTCCATGTTATATT

8248 SEQ ID N0:2452 -10.6 -17.9 55.8 -6.5 -0.6 -4.3 GAACAAAGTCCATGTTATAT

8249 SEQ ID N0:2453 -10.6 -18.4 56.7 -6.5 -1.2 -4.7 AGAACAAAGTCCATGTTATA

8250 SEQ ID N0:2454 -10.6 -18.4 56.9 -6.5 -1.2 -4.7 AATTTATTATGGGAGAATTA

9064 SEQ ID N0:2455 -10.6 -15.7 51.4 -5.1 0 -3.4 TAATTTATTATGGGAGAATT

9065 SEQ ID N0:2456 -10.6 -15.7 51.4 -5.1 0 -3.5 TTTTTTTTTTTTTTTTTCCA

9092 SEQ ID N0:2457 -10.6 -18.7 59.5 -8.1 0 0 CAGTTTTCTTGCTCCATAAT

227 SEQ TD N0:2458 -10.5 -22.4 66.9 -11.9 0 -4.4 CCTTGACAGGTATCTCATCC

274 SEQ ID N0:2459 -10.5 -25.4 73.8 -14.3 -0.3 -2.9 CTCATCATCATTATCTTGTT

590 SEQ ID N0:2460 -10.5 -20.7 64.6 -10.2 0 -1.9 GATATAGTAGGGATCCAGGT

707 SEQ ID N0:2461 -10.5 -23.4 70.1 -12.1 -0.3 -8.9 CTTCTAAGCAAAACCCTCTT

996 SEQ ID N0:2462 -10.5 -22.2 63.7 -11.7 0 -4.1 GGATCACGAAGAAACGTAAA

1018 SEQ ID N0:2463 -10.5 -17.5 52.8 -6.2 -0.6 -6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CAAAAGCAGAATCGCTTGGG

1314 SEQ ID N0:2464 -10.5 -21.4 61.1 -9.1 -1.8 -9.3 ATTGTGCCATTAAAGTAGGA

1348 SEQ ID N0:2465 -10.5 -20.9 62.5 -10.4 0 -3.5 GTAGCCATAGTTGGGGTTTC

1541 SEQ ID N0:2466 -10.5 -25.8 77 -13.8 -1.4 -4.8 TTCACCAAATAAAATGAGCC

1702 SEQ ID N0:2467 -10.5 -18.7 55.5 -8.2 0 -3.2 CTCTCCTTTGTTGTTTCCTT

2009 SEQ ID N0:2468 -10.5 -25.8 76.6 -15.3 0 0 CTCTCTCTCCTTTGTTGTTT

2013 SEQ ID N0:2469 -10.5 -25 76.3 -14.5 0 0 GAGGGGAGCAGAATTTTTTG

2118 SEQ ID N0:2470 -10.5 -21.7 64.9 -11.2 0 -4.1 ATGCATCACAGCAGTCCCAG

2688 SEQ ID N0:2471 -10.5 -27.3 77.1 -15.5 -l.2 -7.5 TGCTCAGTCATGGGGTAGTG

2812 SEQ ID N0:2472 -10.5 -25.8 77.3 -15.3 0 -4.7 CAAGCTCCATTAAACTGAGG

2970 SEQ ID N0:2473 -10.5 -21.1 61.7 -10.1 -0.1 -5 TGAAGACGATGATGGCCAAC

3135 SEQ ID N0:2474 -10.5 -21.9 62 -10.6 0 -9.2 AGTGGAAGAAGTCGTTCATG

3246 SEQ ID N0:2475 -10.5 -21 63.4 -9.2 -1.2 -4.3 ACGCGGAACACAATCAGGAA

3268 SEQ ID N0:2476 -10.5 -22 61.3 -11 0 -7.9 TGGCTTTCTAAAAAAGGCTT

3554 SEQ ID N0:2477 -10.5 -19.6 58.7 -7.6 -1.4 -5.9 TTGGCTTTCTAAAAAAGGCT

3555 SEQ ID N0:2478 -10.5 -19.6 58.7 -7.6 -1.4 -5.9 AATTAAGCTCTTTGCTTATT

3639 SEQ ID N0:2479 -10.5 -19.2 59.7 -5.7 -3 -7.8 GGGGTTGTTTATGAATGACA

3743 SEQ ID N0:2480 -10.5 -21.4 64.1 -10.1 -0.6 -4.3 GCCAATGCACCACTACTGAG

4123 SEQ ID N0:2481 -10.5 -25.7 70.9 -14.4 -0.6 -5 GGCATTAGTGAAATATGTTT

4265 SEQ ID N0:2482 -10.5 -19 58.9 -8.5 0 -4.1 TCAACGATCAAGAAATCTAG

4294 SEQ ID N0:2483 -10.5 -16.6 52.4 -6.1 0 -4.4 ATCAACGATCAAGAAATCTA

4295 SEQ ID N0:2484 -10.5 -16.6 52.3 -6.1 0 -4.9 TTACCCGTTGTCATGTTAAC

4564 SEQ ID N0:2485 -10.5 -22.9 66.3 -12.4 0 -5.8 TTTAACATCTCGTGAATCAA

4751 SEQ ID N0:2486 -10.5 -18.1 55.9 -7.6 0 -4.6 CATGTACAGATTTTCTTCAT

4784 SEQ ID N0:2487 -10.5 -19.9 62 -9.4 0 -6.7 GGTTTCTTGGATCCAAGTTT

4963 SEQ ID N0:2488 -10.5 -23.6 70.4 -9.7 -0.7 -14.9 GACCATGTTGAGGCAGATGA

5075 SEQ TD N0:2489 -10.5 -24.3 70.1 -13.8 0 -4.3 TCTCCAGTGAACAGAACAAT

5167 SEQ ID N0:2490 -10.5 -20.5 60.9 -10 0 -3.9 GAAACGAGCTTCAGCACAAA

5188 SEQ ID N0:2491 -10.5 -20.7 59.9 -9.3 -0.7 -6.6 CTTTTCTATCATCTCAGCCA

5288 SEQ ID N0:2492 -10.5 -24.2 72.1 -13.7 0 -3.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Tnter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CGTGCGGATCCCCTTTGCTC

5381 SEQ TD N0:2493 -10.5 -3l 81.3 -19.8 -0.4 -7.3 CCCTTAACTGAGCTGCCAGG

5668 SEQ ID N0:2494 -10.5 -28.1 76.1 -16.7 -0.6 -9.3 TTCTCCAGGATGACCGCGAT

5770 SEQ ID N0:2495 -10.5 -27.1 73 -15.2 -1.3 -6.7 GGGCAGCTGCAAAATCAGAG

5910 5EQ ID N0:2496 -10.5 -23.7 67.7 -10.3 -0.7 -14 GGCATCCATCTCTCCACTCT

6059 SEQ TD N0:2497 -10.5 -28.9 82.2 -18.4 0 -4 AGACACCTCCTCTTGTTTAC

6161 SEQ ID N0:2498 -10.5 -24.6 72.3 -14.1 0 -3 CTATCATAGGAAGGAGGAGG

6367 SEQ ID N0:2499 -10.5 -21.3 64.2 -10.8 0 -3.4 CATATACTTTACCTTCATAG

6516 SEQ ID N0:2500 -10.5 -19.1 59.3 -8.6 0 -2.4 AGGTAGCCATTGGGTTTCTC

6709 SEQ ID N0:2501 -10.5 -26.2 77.9 -15.1 -0.3 -6.3 TGATCATTTGAGAGACAGGG

6946 SEQ TD N0:2502 -10.5 -21 63.7 -10.5 0 -6 TTTATTAGATTACTTTTTGG

7238 SEQ TD N0:2503 -10.5 -16.8 55 -6.3 0 -2.4 TTTCTAGAATGGATTTATTA

7251 SEQ ID N0:2504 -10.5 -16.6 54.2 -5.6 0 -7.7 CAGGCTATATATATATTTGC

7423 SEQ ID N0:2505 -10.5 -19.1 59.5 -7.9 0 -8.7 TATTAACATGCATTAGTGAT

7549 SEQ ID N0:2506 -10.5 -17.8 56.2 -7.3 0 -6.7 GGCAGTATCCAGTGTGTTTC

7767 SEQ ID N0:2507 -10.5 -25.9 78.3 -15.4 0 -4 AATACCTTATGTATACATAG

8895 SEQ ID N0:2508 -10.5 -17.1 54.5 -5 -0.8 -10.9 CAGGAGGGTTGCTCAAGGTC

906 SEQ TD N0:2509 -10.4 -26.1 76.9 -13.7 -2 -4.5 CTTTGTCCAGTCAGGAGGGT

917 SEQ ID N0:2510 -10.4 -27.1 80.2 -15.8 -0.8 -5.3 ATTTTTATAAGTGACTCAAA

970 SEQ ID N0:2511 -10.4 -15.7 51.6 -5.3 0 -4.7 ATTCCTCAGATTGCCCATGA

1277 SEQ TD N0:2512 -10.4 -26.3 73.6 -15.9 0 -4.5 CCTTCCAGTTAAATGTGCTC

1401 SEQ ID N0:2513 -10.4 -24 69.6 -13.6 0 -5.1 AGGTGGCCTGATTCTGCTCC

1755 5EQ ID N0:2514 -10.4 -29.4 83.2 -19 0 -7.2 CAGAATTTTTTGTCACTGGT

2110 SEQ ID N0:2515 -10.4 -20.9 63.9 -10.5 0 -3.5 TGGCCTGACTAACGTTACTG

2337 SEQ ID N0:2516 -10.4 -23.7 67.1 -12.8 0 -7.9 ACTGGCCTGACTAACGTTAC

2339 SEQ ID N0:2517 -10.4 -23.9 67.8 -13 0 -7.9 GTGCCCTCTGGGGGAAGTTG

2476 SEQ ID N0:2518 -10.4 -29.2 81.1 -16.6 -2.2 -8.7 GAGGGTATTTAAGACAATGC

2777 SEQ ID N0:2519 -10.4 -19.7 60.2 -9.3 0 -2.9 TCCCGCATCTTATTTTTCAC

3523 SEQ ID N0:2520 -10.4 -24.5 70.1 -14.1 0 -3.6 CACCCTTCAGTAAAACAAGC

3970 SEQ ID N0:2521 -10.4 -21.8 62.5 -11.4 0 -2.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGCCTTCTTCTGTACTTACT

4014 SEQ ID N0:2522 -10.4 -24.4 72.9 -14 0 -4.8 TTGCCTTCTTCTGTACTTAC

4015 SEQ ID N0:2523 -10.4 -23.6 71.2 -13.2 0 -4.8 AGGCATTAGTGAAATATGTT

4266 SEQ ID N0:2524 -10.4 -18.9 58.7 -8.5 0 -4 CAAAGAAACATCAACGATCA

4304 SEQ ID N0:2525 -10.4 -16.5 51.2 -6.1 0 -4.9 GGATAAGGCTCTTAGAGGTC

4400 SEQ ID N0:2526 -10.4 -23.2 70.5 -12.2 -0.3 -6.1 ATGGTGACCATGTTGAGGCA

5080 SEQ ID N0:2527 -10.4 -25.5 73 -14 -1 -7.l GTAGTAGTGTCTGAGGGAAA

5204 SEQ ID N0:2528 -10.4 -21.7 66.8 -11.3 0 -2.9 CATCAATTCCAGCTTCCTTT

5514 SEQ ID N0:2529 -10.4 -24.6 70.7 -14.2 0 -4.5 GCTGCCAGGGTGAATTGTGT

5657 SEQ ID N0:2530 -10.4 -27.2 77.3 -16.8 0 -4.4 GTTCACCACAACCAGGAAGG

5744 SEQ ID N0:2531 -10.4 -24.7 69.1 -13.8 -0.2 -4 TCACTCAGGGGCTCTGCACT

5812 SEQ TD N0:2532 -10.4 -28.5 82 -17.5 -0.3 -4.8 ATGGGCAGATCCATGGCAAT

5974 SEQ ID N0:2533 -10.4 -25.6 71.5 -14 -1.1 -8 CTGGAGTGGAGTTCCCATTT

6318 SEQ ID N0:2534 -10.4 -26.3 75.7 -14.6 -1.2 -6.7 ACCTCTTTTCCTTTGCTTTC

6436 SEQ ID N0:2535 -10.4 -25.5 75.2 -15.1 0 -3.6 TGACCTCTTTTCCTTTGCTT

6438 SEQ ID N0:2536 -10.4 -25.6 74.2 -15.2 0 -3.6 TCCAGTTGACACATATACTT

6527 SEQ ID N0:2537 -10.4 -21.4 64.3 -10.5 -0.2 -3.6 GCCATCTAGAAAATGGAAGT

7043 SEQ ID N0:2538 -10.4 -20.3 60 -8.3 -1.6 -8.3 AATTAAATATAGAGCTCTGA

7203 5EQ ID N0:2539 -10.4 -16.3 52.7 -4.9 0 -10 TGCATTAGTGATAGCCTTTA

7541 SEQ ID N0:2540 -10.4 -22.4 67.1 -12 0 -4.7 ATATTTAAACAAGTTTTAGA

7827 SEQ ID N0:2541 -10.4 -14.2 48.5 -3.8 0 -5.5 AATAAAAGGTTATATTGAGG

8137 SEQ TD N0:2542 -10.4 -14.7 49 -3.7 -0.3 -2.8 CTACAGTAAATAGTTTTGTA

8367 SEQ ID N0:2543 -10.4 -17.1 55.4 -6.7 0 -4.1 ATTTATTCTTTTGGCTCAAT

8449 SEQ ID N0:2544 -10.4 -19.9 61.6 -9.5 0 -4.9 GTTACTACTATTATTATCAA

8727 SEQ ID N0:2545 -10.4 -16.6 54.3 -6.2 0 -1.7 TAATACCTTATGTATACATA

ggg6 SEQ ID N0:2546 -10.4 -16.8 53.8 -5 -0.8 -10.5 TATCATTAATACCTTATGTA

gg02 SEQ ID N0:2547 -10,4 -17.4 55.4 -7 0 -4.2 CAGCTTTTTTTTTTTTTTTT

115 SEQ ID N0:2548 -10.3 -18.9 60.3 -8.6 0 -4.5 TGTCAAACCTTGATGTGGCT

253 SEQ ID N0:2549 -10.3 -23.8 68.5 -12.3 -1.1 -5.8 GTAGCTTCTTGCATACGAAT

425 SEQ ID N0:2550 -10.3 -22.2 65.5 -10.3 -1.5 -5.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATCCTGCACATTTAATTACG

446 SEQ ID N0:2551 -10.3 -20.7 60.8 -10.4 0 -4.8 ACAGTTGGTCAAAATAGTGC

878 SEQ ID N0:2552 -10.3 -20.3 61.9 -10 0 -3.8 TCATAAATACACAGTTGGTC

888 SEQ ID N0:2553 -10.3 -19 59.2 -8,7 0 -2.8 CCTCTTGCCAAGATTTTTAT

982 SEQ ID N0:2554 -10.3 -22.8 66.3 -11.7 -0.6 -6.3 AAATCCAGCCAGTTCCATGG

1036 SEQ ID N0:2555 -10.3 -25.8 71.5 -14.8 -0.4 -7.7 TGAACAGCTGCAGCCCAATG

1260 SEQ ID N0:2556 -10.3 -25.8 70.5 -13 0 -13.2 CACAGATGTATCCTTCTGGA

1509 SEQ ID N0:2557 -10.3 -23.6 69.6 -11.7 -1.6 -5.9 ACGTAATGTCAACTGGTAAA

1634 SEQ ID N0:2558 -10.3 -18.6 56.5 -8.3 0 -4.4 TATCATGTATGTTTTCCCAG

1658 SEQ ID N0:2559 -10.3 -22.3 66.9 -12 0 -4.7 CTTTGATGCTTCTGAAGAAC

1922 SEQ ID N0:2560 -10.3 -19.8 60.4 -9 0 -8.3 TGTCACTGGTCAGTCTGTTT

2100 SEQ TD N0:2561 -10.3 -24.8 76.5 -14.5 0 -6.6 TTGTCACTGGTCAGTCTGTT

2101 5EQ ID NO:2562 -10.3 -24.8 76.5 -14.5 0 -6.4 TTTGTCACTGGTCAGTCTGT

2102 SEQ ID N0:2563 -10.3 -24.8 76.5 -14.5 0 -6.6 AGAATTTTTTGTCACTGGTC

2109 SEQ ID N0:2564 -10.3 -20.6 64.2 -10.3 0 -3.3 CCAGCATCTCCATTGAAATC

2541 SEQ ID N0:2565 -10.3 -23.3 66.7 -13 0 -4.1 TTTGGCTTTCTAAAAAAGGC

3556 5EQ ID N0:2566 -10.3 -18.8 57.2 -7.6 -0.7 -4.5 TCTATCTTATTGCCTTCATG

3589 SEQ ID N0:2567 -10.3 -22.1 66.8 -11.8 0 -4.1 TCTAAGATAATTAAGCTCTT

3647 SEQ ID N0:2568 -10.3 -17.1 55 -6.8 0 -5.9 TTCTCGGGGTAGAACAACAT

3905 SEQ ID N0:2569 -10.3 -22.1 64.4 -11.3 -0.1 -3.7 TTCTGTACTTACTTGACAGA

4007 SEQ ID N0:2570 -10.3 -20.5 63.6 -8.2 -2 -6.5 GAAACATCAACGATCAAGAA

4300 SEQ ID N0:2571 -10.3 -16.4 51.2 -6.1 0 -4.9 AGAGCATTCACAACCACCCT

4435 SEQ ID N0:2572 -10.3 -26.5 72.5 -15.6 -0.3 -4.1 ACAGCACATTCATGATAGAG

4470 SEQ ID N0:2573 -10.3 -20.4 62.2 -10.1 0 -6.4 AATATCCATCCAGCCTTTAA

4718 SEQ ID N0:2574 -10.3 -23.1 65.8 -12.8 0 -3.2 CTGACCATGGGCAGATCCAT

5980 SEQ ID N0:2575 -10.3 -27 74.4 -15.5 -1.1 -8.8 TCACCACTGACCATGGGCAG

5986 SEQ ID N0:2576 -10.3 -27.5 75.4 -15.7 -1.4 -8.9 ATGATAGCGGCAGACACCTC

6172 SEQ ID N0:2577 -10.3 -25.6 71.9 -15.3 0 -6.6 TGTATAGGCACTGACTATGA

6591 SEQ ID N0:2578 -10.3 -21.4 64.6 -10.5 -0.3 -4.8 AATAGAATGGATGCAAGGAC

6789 SEQ ID N0:2579 -10.3 -18.5 56.4 -8.2 0 -5.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGAATAAACTATGAATTGGG

6867 SEQ ID N0:2580 -10.3 -15.4 49.9 -5.1 0 -3.1 GTATACTGTTTCTTCAACGT

6921 SEQ ID N0:2581 -10.3 -21.1 64.3 -9.7 -1 -7 TACAATTCTTTTTCTGGTTT

7005 SEQ ID N0:2582 -10.3 -19.6 61.5 -9.3 0 -2.6 AGCCATCTAGAAAATGGAAG

7044 SEQ ID N0:2583 -10.3 -19.1 57.4 -7.2 -1,6 -8.3 ATTAGAGGAGACTTTACAGG

7120 SEQ ID N0:2584 -10.3 -19.9 61.8 -9.1 -O.l -5.5 AAAATAATCCTTTAAATATT

7137 SEQ ID N0:2585 -10.3 -12.5 44.3 -2.2 0 -4.4 ATATAGAGCTCTGAAACTTA

7197 SEQ ID N0:2586 -10.3 -18.1 57 -6.8 0 -10 AAAGCATTTGACCTAAATTA

7218 SEQ ID N0:2587 -10.3 -17.1 53.2 -6.3 -0.1 -4 CTGTTGGAAGCTTGCAAAAG

7488 SEQ ID N0:2588 -10.3 -20.5 60.6 -8.5 -1.7 -7 TGATACAGGATACATCCCTG

7505 SEQ ID N0:2589 -10.3 -22.9 66.2 -11.4 -1.1 -6.9 TTAGGAAATTATCCATTGAA

7997 SEQ ID N0:2590 -10.3 -17.1 53.7 -4.6 -2.2 -7.2 CTAACTTTTATTTTAGGAAA

8009 SEQ ID N0:2591 -10.3 -15.4 50.8 -5.1 0 -2.8 ATCTAACTTTTATTTTAGGA

8011 SEQ ID N0:2592 -10.3 -17.2 55.7 -6.9 0 -3.2 TGTAATAGCTTTAGTGCAGC

8228 SEQ ID N0:2593 -10.3 -22 67.2 -10.8 -0.8 -5.6 GTTCTTCAATACACTACAGT

8380 SEQ ID N0:2594 -10.3 -20.6 63.7 -10.3 0 -3.1 GAAAGTTGGAACATGTGAAC

8703 SEQ ID N0:2595 -10.3 -17.8 55.1 -6.7 -0.6 -7 AAGCTATTGTAGGTGGTTAC

8742 SEQ TD N0:2596 -10.3 -21.5 66.2 -11.2 0 -5.1 AAGCTTTCAGGTCCTGGGGG

493 SEQ ID N0:2597 -10.2 -27.8 79.2 -16.8 -0.4 -9 AAGTGACTCAAAGGTATAGA

962 SEQ ID N0:2598 -10.2 -18.3 57.4 -8.1 0 -3.1 GCAAAACCCTCTTGCCAAGA

989 SEQ ID N0:2599 -10.2 -25.3 68.3 -13.8 -1.2 -6.9 AACAGCTGCAGCCCAATGAG

1258 SEQ ID N0:2600 -10.2 -25.8 70.9 -13.1 0 -13.2 TAACAAATGTCCCATTTGAA

1371 SEQ ID N0:2601 -10.2 -18.9 56.4 -4.9 -3.8 -10.4 TCTTGAGTCATGAGTCGAAA

1600 SEQ ID N0:2602 -10.2 -20.5 62.2 -8.6 0 -11.6 CCCAATAGTCTTGAGTCATG

1608 SEQ ID N0:2603 -10.2 -23.2 68 -12.5 -0.2 -5.8 AGCTGCTTCTTTTGACGCTG

2061 SEQ ID N0:2604 -10.2 -25.4 73.2 -13.2 -2 -7.5 CAGATCCAACATCCTTTGCC

2220 SEQ ID N0:2605 -10.2 -25.9 71.8 -15.2 -0.1 -4.5 AAGAACACATTGGCAAATCT

2665 SEQ ID N0:2606 -10.2 -18.7 56.5 -8.5 0 -4.4 ATGATCGCAGTACAGACAAT

3009 SEQ ID N0:2607 -10.2 -20.9 61.9 -10 -0.4 -5.7 CTGTACTTACTTGACAGAAT

4005 SEQ ID N0:2608 -10.2 -19.3 59.6 -8.2 -0.8 -4.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCTCAAACCAGTTGTGCTCA

4080 SEQ ID N0:2609 -10.2 -24.2 70.6 -13.3 -0.4 -5.2 AGTCTCAAACCAGTTGTGCT

4082 SEQ ID N0:2610 -10.2 -24.3 71.5 -14.1 0.2 -4.9 AGTGGTAGAACTTGCCAGCA

4542 SEQ ID N0:2611 -10.2 -25.4 73.3 -13.9 -l.2 -6.4 CTTTGATCAGACGTAGGATT

5361 SEQ ID N0:2612 -10.2 -21.2 63.7 -11 0 -6.5 AAAGAGCAGCGTGCGGATCC

5390 SEQ ID N0:2613 -10.2 -26.3 71.4 -14.4 -1.4 -11.1 CAAAGAGCAGCGTGCGGATC

5391 SEQ ID N0:2614 -10.2 -25 69.1 -12.6 -2.2 -8.5 AGCAGGAGGCCGATGTTAAA

5437 SEQ ID NO:2615 -10.2 -24.3 68.1 -13.2 -0.8 -7.7 TGGACTTTGTTGGGTTTTGC

5950 SEQ ID N0:2616 -10.2 -24.1 71.9 -13.9 0 -2.6 TTTAATTGCCTCTTTGTTAT

6251 SEQ ID N0:2617 -10.2 -20.5 62.7 -10.3 0 -3 GGTAAGTCAATCCTCCCTTT

6268 SEQ ID N0:2618 -10.2 -25.8 73.5 -15.6 0 -2.5 TTACTTTTGATTTTCTCTGA

6455 SEQ ID N0:2619 -10.2 -19.3 61.2 -9.l 0 -2.2 GTATAGGCACTGACTATGAG

6590 SEQ ID NO:2620 -10.2 -21.4 65 -10.5 -0.4 -5.5 GTTCACTTTGCACAACTATC

6739 SEQ ID N0:2621 -10.2 -21.6 65.4 -11.4 0 -5 AGCATTATGAATAAACTATG

6874 SEQ ID N0:2622 -10.2 -15.3 50.1 -5.1 0 -4.1 CATAGCATTATGAATAAACT

6877 SEQ ID N0:2623 -10.2 -16 51.4 -5.1 -0.5 -4.2 CTTGTATACTGTTTCTTCAA

6924 SEQ ID N0:2624 -10.2 -19.9 62.3 -9.7 0 -6.3 CTGATCATTTGAGAGACAGG

6947 SEQ TD N0:2625 -10.2 -20.7 63.1 -10.5 0 -6.2 ATTACTTTTTGGAAAGCATT

7230 SEQ ID N0:2626 -10.2 -18.3 57 -6.5 -l.6 -5.5 TATTAGATTACTTTTTGGAA

7236 SEQ ID N0:2627 -10.2 -16.5 53.7 -6.3 0 -2.4 GACATAGACTATCAGCTTTG

7348 SEQ ID N0:2628 -10.2 -20.6 63.2 -10.4 0 -4.5 TGACATAGACTATCAGCTTT

7349 SEQ ID N0:2629 -10.2 -20.6 63.2 -10.4 0 -4.5 TTGACATAGACTATCAGCTT

7350 SEQ ID N0:2630 -10.2 -20.6 63.2 -10.4 0 -4.5 TGGAAGCTTGCAAAAGACAC

7484 SEQ ID N0:2631 -10.2 -20 59.1 -7.7 -2.1 -7 CCTGTTGGAAGCTTGCAAAA

7489 SEQ ID N0:2632 -10.2 -22.5 64 -12.3 0 -7.2 TTTAGACATATTTTTAGCAA

7813 SEQ ID N0:2633 -10.2 -17 54.8 -6.8 0 -4.1 AAGAACAAAGTCCATGTTAT

8251 SEQ ID N0:2634 -10.2 -18 55.6 -6.5 -l.2 -4.7 TTTTAGCAATAGCAATTCCC

8408 SEQ ID N0:2635 -10.2 -21.8 63.8 -10.6 -0.9 -4.5 ATTATATTGAAAGAAGATGA

8801 SEQ ID N0:2636 -10.2 -13.9 47.4 -3.7 0 -2.2 CCTCACATAAATCCAAGGGT

8959 SEQ ID N0:2637 -10.2 -23.2 65.7 -13 0 -3.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGTGGCTTGGCTTCAGTTTT

240 SEQ ID N0:2638 -10.1 -25.8 76.9 -15 -0.4 -6 CTGTGAATTATCTCATTTAT

356 SEQ ID N0:2639 -10.1 -18.1 57.5 -8 0.4 -2.8 TAAAAGTTTTCTTATTGATA

723 SEQ ID N0:2640 -10.1 -14.6 49.5 -3.4 -1 -6.3 CCAAAATCTTGATAGCAATT

828 SEQ ID N0:2641 -10.1 -18.2 55.3 -7.5 -0.3 -4.4 AGCCCAATGAGAGCAAACAC

1249 SEQ ID N0:2642 -10.1 -23.2 65 -12.3 -0.6 -4.1 AACATTTATTCCTCAGATTG

1284 SEQ ID N0:2643 -10.1 -19.3 59.4 -9.2 0 -2.5 AACATAAAAGTGACTGTCAT

1433 SEQ ID N0:2644 -10.1 -17 53.7 -6.2 -O.l -8.8 CGTAATGTCAACTGGTAAAG

1633 SEQ ID N0:2645 -10.1 -18.4 56.2 -8.3 0 -3.2 GGTGCTCTCTCTGTCTTCTT

1986 SEQ ID N0:2646 -10.1 -27 83.3 -16.9 0 -3.6 AAGCTGCTTCTTTTGACGCT

2062 SEQ ID N0:2647 -10.1 -24.7 70.9 -13.2 -1.3 -8.6 GGTCAGTCTGTTTCCATCCA

2093 SEQ ID N0:2648 -10.1 -27.6 81.1 -17.5 0 -3.6 TCTTGGGGAAAACAGGGAGC

2162 SEQ ID N0:2649 -10.1 -23.2 66.7 -13.1 0 -2.8 CTATTGCGTCTTGGGGAAAA

2170 SEQ ID N0:2650 -10.1 -22 63.3 -7.1.9 0 -4.5 TCTCCTGCTTTCGCCGTCTT

2276 SEQ ID N0:2651 -10.1 -29.9 82.1 -19.1 -0.5 -3.6 GCATCTTCCCATTTGCTGGA

2385 SEQ ID N0:2652 -10.1 -27.7 77.6 -16.5 -l -5.2 CAGCATCTCCATTGAAATCT

2540 SEQ ID N0:2653 -10.1 -22.2 65 -12.1 0 -4.1 CTTTTAACCATGCATCACAG

2697 SEQ ID N0:2654 -10.1 -21.6 63.4 -11.5 0 -6.6 CTCCACATTTGACAGACCAA

2987 SEQ ID N0:2655 -10.1 -23.4 66.3 -13.3 0 -2.7 AGGTTTCCTAGAGCCCCCAC

3106 SEQ ID N0:2656 -10.1 -30.9 83.6 -20.8 0 -3.8 TCACATAATCAATTCCCTTT

3507 SEQ ID N0:2657 -10.1 -21 62 -10.9 0 -2.5 TTCACATAATCAATTCCCTT

3508 SEQ ID N0:2658 -10.1 -21 62 -10.9 , 0 -2.5 TAATTAAGCTCTTTGCTTAT

3640 SEQ ID N0:2659 -10.1 -18.8 58.8 -5.7 -3 -8 TTGCTTTCTTCTAGTTCTGA

3841 SEQ ID N0:2660 -10.1 -22.6 70.4 -12.5 0 -4 TTTCTCTTTGCTTTCTTCTA

3848 SEQ TD N0:2661 -10.1 -22.3 69.9 -12.2 0 -3.6 CTTCTTCGGGTTCAGTTTCA

3936 SEQ ID N0:2662 -10.1 -24.9 74.6 -14.8 0 -3 ACCCTTCAGTAAAACAAGCT

3969 SEQ ID N0:2663 -10.1 -22 63.2 -11.9 0 -4.8 TCATATACAGGCTGAAGTTT

4768 SEQ ID N0:2664 -10.1 -20.6 63 -10.5 0 -4.1 TTCATATACAGGCTGAAGTT

4769 SEQ ID N0:2665 -10.1 -20.6 63 -10.5 0 -4.1 AGGTTTCTTGGATCCAAGTT

4964 SEQ ID N0:2666 -10.1 -23.5 70.3 -9.7 -1.3 -15.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTTCTATCATCTCAGCCAGA

5286 SEQ ID N0:2667 -10.1 -23.8 71.4 -13.7 0 -3,4 CAGCAGAGGTTGTAATTTGG

5580 SEQ ID N0:2668 -10.1 -21.9 65.9 -11.8 0 -5.7 AAACTGGGTCGCATCGGGAT

5873 SEQ ID N0:2669 -10.1 -25.2 69.1 -15.1 0 -3.6 CCTCCTCTTGTTTACGTTTC

6156 SEQ ID N0:2670 -10.1 -25.5 74.2 -15.4 0 -4.8 TAGGTAAGTCAATCCTCCCT

6270 SEQ ID N0:2671 -10.1 -25.3 72.4 -14.7 -0.2 -4.8 GCTTTCTTTTTCTGGTTTGT

6422 SEQ ID N0:2672 -10.1 -23.7 73.2 -13.6 0 -2.8 CCTTTAAATATTAGAGGAGA

7129 SEQ ID N0:2673 -10.1 -17.9 55.9 -6.9 -0.7 -5.2 ACCATATGATACTCATGTTG

7729 SEQ TD N0:2674 -10.1 -20.4 61.7 -9.6 -0.5 -6 AATTACGTGTAGCTTCTTGC

433 SEQ ID N0:2675 -10 -22.2 66.4 -11.5 -0.4 -5.8 GTTCTTTCCAGCTTCCAAGT

632 SEQ ID N0:2676 -10 -26.3 77 -16.3 0 -4.5 TCATTACTATAAAAGTTTTC

732 SEQ ID N0:2677 -10 -15.2 51 -5.2 0 -6.3 ATACACAGTTGGTCAAAATA

882 SEQ ID N0:2678 -10 -17.9 55.9 -7.9 0 -3.8 AAGATTTTTATAAGTGACTC

973 SEQ ID N0:2679 -10 -16.3 53.6 -6.3 0 -4.1 TGGGGGCCACTGCAAACATT

1298 SEQ ID N0:2680 -10 -26.5 72 -15.7 -0.6 -7.7 TTGTGTAGCCATAGTTGGGG

1545 SEQ ID N0:2681 -10 -25.3 74.4 -13.8 -1.4 -4.8 TGTCAACTGGTAAAGATTTT

1628 SEQ ID N0:2682 -10 -18.3 57.2 -8.3 0 -2.7 TGTTCTGCTTCTTCCAAGGT

1771 SEQ ID N0:2683 -l0 -25.6 75.7 -15.1 -0.1 -3.6 GTCTCTCTCTCCTTTGTTGT

2015 5EQ ID N0:2684 -10 -26.4 81.5 -16.4 0 0 GTCTGTTTCCATCCATGGAG

2088 5EQ ID N0:2685 -10 -25.9 75.5 -14.2 -l.7 -10.4 TCTCCATGTCTGTGCGGCAC

2308 SEQ ID N0:2686 -10 -28.6 80.5 -17.1 -0.2 -10.9 TGGGGGAAGTTGTCCAGTAG

2468 SEQ ID N0:2687 -10 -25.2 73.9 -13.2 -2 -6.1 TTTCTGACTTCCGTTTCAGT

2500 SEQ ID N0:2688 -10 -24.2 71.8 -13 -1.1 -5.7 CAAGAACACATTGGCAAATC

2666 SEQ ID N0:2689 -10 -18.5 55.9 -8.5 0 -4.4 TCAAGAACACATTGGCAAAT

2667 SEQ ID N0:2690 -10 -18.5 55.9 -8.5 0 -4.4 ATCAAGAACACATTGGCAAA

2668 SEQ ID N0:2691 -10 -18.5 55.9 -8.5 0 -4.4 TTAACCATGCATCACAGCAG

2694 SEQ ID N0:2692 -10 -23 66.3 -11.7 -1.2 -6.6 AGGGTATTTAAGACAATGCA

2776 SEQ ID N0:2693 -10 -19.8 60.1 -9.3 -0.1 -52 ATTGCTCAGTCATGGGGTAG

2814 SEQ ID N0:2694 -10 -24.7 74.1 -14.7 0 -4.7 AATAAGGCACATGGTTTGGC

3338 SEQ ID N0:2695 -10 -22.5 65.7 -11.9 -0.3 -5,2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTCCAATGACCATGACCAAC

3366 SEQ ID N0:2696 -10 -23 64.2 -13 0 -4.5 TTCCCTTTTGCATTCTTCCT

3495 SEQ ID N0:2697 -10 -27 76.9 -17 0 -5.l CTTTTGGCTTTCTAAAAAAG

3558 SEQ ID N0:2698 -10 -16.8 53.2 -5.7 -1 -5.7 ACTTTTTAATACACCCTTCA

3981 SEQ TD N0:2699 -10 -21.4 63.2 -11.4 0 -2.2 CTTCCCTTTGCCTTCTTCTG

4022 SEQ ID N0:2700 -10 -28 79.2 -18 0 -3 CCAGCACCAGGCATTAGTGA

4274 SEQ ID N0:2701 -10 -27.4 76.3 -15.8 -1.5 -5.4 ACGATCAAGAAATCTAGCCA

4291 SEQ ID N0:2702 -10 -20.7 60.2 -10.7 0 -4.9 CTAACCAAAGAAACATCAAC

4309 SEQ ID N0:2703 -10 -16.1 50.6 -6.1 0 -2.9 AATGTCCGTAATGATTTGAT

4369 SEQ ID N0:2704 -10 -19.3 58.1 -9.3 0 -2.6 TAAGGCTCTTAGAGGTCTTA

4397 SEQ ID N0:2705 -10 -22.1 68.1 -11.5 -0.3 -5.4 CATTCACAACCACCCTCATG

4431 SEQ ID N0:2706 -10 -25.2 69.2 -15.2 0 -4.l CTCCAACAAGAGCATTCACA

4443 SEQ ID N0:2707 -10 -22.7 65.4 -12.1 -0.3 -4.1 GTCAAACATGTTACCCGTTG

4574 SEQ ID N0:2708 -10 -23.1 65.6 -12.4 0 -9 TTAACATCTCGTGAATCAAC

4750 SEQ TD N0:2709 -10 -18.2 56.1 -8.2 0 -4.6 TTGTTTGCTGGGCGAGGTAT

4993 SEQ ID N0:2710 -10 -25.9 74.3 -15.1 -0.6 -4.4 CCATTCCTTGGAATTTGTTT

5007 SEQ ID N0:2711 -10 -22.9 66.6 -11.2 -1.7 -8.4 AAGACCATTCCTTGGAATTT

5011 SEQ ID N0:2712 -10 -21.6 62.9 -9.9 -1.7 -8.4 ACTTTTCTATCATCTCAGCC

5289 SEQ ID NO:2713 -10 -23.7 71.6 -13.7 0 -3.2 TGGACATCCCAAAGATGGCA

5478 SEQ ID N0:2714 -10 -24.7 68.2 -12.4 -2.3 -8 AGGATGACCGCGATGTACAT

5764 SEQ ID N0:2715 -10 -24.4 67.5 -12.9 -1.3 -10.1 CTGCACTTTCTTCAGTAGCA

5799 SEQ ID N0:2716 -10 -24.6 73.8 -13.1 -1.4 -5.5 TCTCAAAGTCATCCTCACTC

5826 SEQ ID NO:2717 -10 -23.2 69.7 -13.2 0 -2.6 ATTCGAAGGGCATCCATCTC

6067 SEQ ID N0:2718 -10 -24.9 71 -13.7 -1.1 -8.2 CTCTTGTTTACGTTTCAAAG

6152 SEQ TD N0:2719 -10 -19.5 60 -9.5 0 -4.8 TATCATAGGAAGGAGGAGGG

6366 SEQ ID N0:2720 -10 -21.6 64.8 -11.6 0 -3.4 CACCTTTGTCTGATCATTTG

6956 SEQ ID N0:2721 -l0 -22.4 66.9 -12.4 0 -6.2 CAATTCTTTTTCTGGTTTTG

7003 SEQ ID N0:2722 -10 -19.8 61.8 -9.8 0 -2.5 ATGCATTAGTGATAGCCTTT

7542 SEQ ID N0:2723 -10 -22.7 67.7 -12.7 0 -6.6 TTCTTTAACCAAGACTTGTG

7605 SEQ ID N0:2724 -10 -19.8 60.3 -8.8 -0.9 -5.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Tnter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTATCTAACTTTTATTTTAG

8013 SEQ ID N0:2725 -10 -15.2 51.5 -5.2 0 -3 CCATTCTTTTTAGCAATAGC

8415 5EQ ID N0:2726 -10 -21.5 64.5 -10.7 -0.6 -4.1 CTCACCATTCTTTTTAGCAA

8419 SEQ ID N0:2727 -10 -22.2 65.9 -12.2 0 -4.l AGTTGGAACATGTGAACTTG

8700 SEQ ID N0:2728 -10 -19.6 59.8 -8.8 -0.6 -7.8 TTATTATGGGAGAATTATGA

9061 SEQ ID N0:2729 -10 -16.9 54.1 -6.9 0 -3.2 TATAACGTGTCTTCCTCTGG

143 SEQ ID N0:2730 -9.9 -23.3 68.4 -13.4 0 -5.3 TCAGTTTTCTTGCTCCATAA

228 SEQ ID N0:2731 -9.9 -22.8 68.5 -12.9 0 -4.4 CATTACTATAAAAGTTTTCT

731 SEQ ID N0:2732 -9.9 -15.7 51.7 -5.2 -0.3 -6.3 AGGTCATAAATACACAGTTG

891 SEQ ID N0:2733 -9.9 -18.6 58 -8.7 0 -2.8 TTTATTCCTCAGATTGCCCA

1280 SEQ ID N0:2734 -9.9 -25.6 72.6 -15.7 0 -3 CAAATGTCCCATTTGAATCC

1368 SEQ ID N0:2735 -9.9 -22.1 62.9 -9.3 -2.9 -8.9 ATGTCAACTGGTAAAGATTT

1629 SEQ ID N0:2736 -9.9 -18.2 56.9 -8.3 0 -3.2 GTAATGTCAACTGGTAAAGA

1632 SEQ TD N0:2737 -9.9 -18.2 56.7 -8.3 0 -3.2 CTCATAGGCCATGGCCACCA

1736 SEQ ID N0:2738 -9.9 -30 80.1 -15.5 -2.5 -17.4 GTTCTGCTTCTTCCAAGGTG

1770 SEQ ID N0:2739 -9.9 -25.6 75.7 -15.2 -0.1 -3.6 GTTCATCATCAGCAAAGTCA

2244 SEQ ID N0:2740 -9.9 -22 66.7 -12.1 0 -4.1 GCGTCGCTCTCCATGTCTGT

2315 SEQ ID N0:2741 -9.9 -29.8 83.4 -19.9 0 -6.1 TACTGGCCTGACTAACGTTA

2340 SEQ ID N0:2742 -9.9 -23.4 66.7 -13 0 -7.8 CTGGAAGCCCTGGCACCATC

2370 SEQ ID N0:2743 -9.9 -29.7 79.2 -18.2 -1.5 -10.6 ATTGCAATCCACAGTGCTGT

2405 SEQ ID N0:2744 -9.9 -25 71.9 -13.5 -1.5 -9 TTACTTTTAACCATGCATCA

2700 SEQ ID N0:2745 -9.9 -20.7 61.8 -10.8 0 -6.6 GAATTGCCAATGATCTTAAT

3085 SEQ ID N0:2746 -9.9 -18.8 56.9 -8.9 0 -4.9 AGAATTGCCAATGATCTTAA

3086 5EQ ID N0:2747 -9.9 -18.8 57 -8.9 0 -4.9 GAAGACGATGATGGCCAACA

3134 5EQ ID N0:2748 -9.9 -22.6 63.2 -12.7 0 -6 GTGGAAGAAGTCGTTCATGT

3245 SEQ ID N0:2749 -9.9 -22.2 66.4 -11 -1.2 -4.9 TGTGCTTCCTTCAGATGAGC

3881 SEQ ID N0:2750 -9,9 -25.5 75.2 -14.9 -0.5 -4 TCTTCGGGTTCAGTTTCAGC

3934 SEQ ID N0:2751 -9.9 -25.7 77.1 -15.8 0 -3 TAACCAAAGAAACATCAACG

4308 SEQ ID N0:2752 -9.9 -16 49,8 -6.1 0 -3 GGCTAACCAAAGAAACATCA

4311 SEQ ID N0:2753 -9.9 -19.6 57.5 -9 -0.5 -3.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ACCCTCATGCCTTCAAACCG

4420 SEQ ID N0:2754 -9.9 -27.7 72.4 -17.8 0 -3.8 AGTTTAACATCTCGTGAATC

4753 SEQ ID N0:2755 -9.9 -19.3 59.7 -9.4 0 -3.9 TGACCATGTTGAGGCAGATG

5076 SEQ ID N0:2756 -9.9 -23.7 68.7 -13.8 0 -4.3 AAACGAGCTTCAGCACAAAT

5187 5EQ 2D N0:2757 -9.9 -20.1 58.7 -9.3 -0.7 -6.6 GCAAAGTTGGACATCCCAAA

5485 SEQ ID N0:2758 -9.9 -22.8 64 -10.7 -2.2 -7.6 GGTGAATTGTGTCAGGGTCA

5649 SEQ ID N0:2759 -9.9 -24.6 73.6 -14.7 0 -3.1 GTCTCCCTTAACTGAGCTGC

5672 SEQ ID N0:2760 -9.9 -27.1 77.4 -17.2 0 -5.2 CAGGATGACCGCGATGTACA

5765 SEQ ID N0:2761 -9.9 -25.1 68.5 -13.8 -1.3 -8.1 ACTGACCATGGGCAGATCCA

5981 SEQ ID N0:2762 -9.9 -27.2 75 -15.5 -1.8 -8.5 CTGAATGATAGCGGCAGACA

6176 SEQ ID N0:2763 -9.9 -22.9 65.3 -13 0 -6.6 TATAGGTAAGTCAATCCTCC

6272 SEQ ID N0:2764 -9.9 -22.1 66.1 -11.7 -0.2 -3.5 TTTCTTTTTACTTTTGATTT

6462 SEQ ID N0:2765 -9.9 -17.8 57.7 -7.9 0 -2.2 AATGTTCACTTTGCACAACT

6742 SEQ ID N0:2766 -9.9 -20.8 62.2 -10.9 0 -5 ACTAAAGGTGTTTGGTGTAG

6766 SEQ ID N0:2767 -9.9 -20.5 63.4 -9.9 -0.5 -4.7 TTTCTTCAACGTAAACCTCA

6913 SEQ ID N0:2768 -9.9 -20.7 61 -10.8 0 -5.3 GATTTATTAGATTACTTTTT

7240 SEQ ID N0:2769 -9.9 -16.2 53.7 -6.3 0 -2.3 TTGCTGAGAGCAGAAGATGG

7331 SEQ ID N0:2770 -9.9 -22.2 65.8 -10.2 -2.1 -6.9 TTAGTGATAGCCTTTAAACT

7537 SEQ ID N0:2771 -9.9 -19.6 60 -9.7 0 -4.2 TAGGCAATATTAACATGCAT

7556 SEQ ID N0:2772 -9.9 -18.9 57.5 -7 -2 -6.8 AATTGTGATGTCATCAACCT

7910 SEQ ID N0:2773 -9.9 -21 62.7 -10.4 -0.5 -7.2 GCTTTAGTGCAGCAGGAATT

8221 SEQ ID N0:2774 -9.9 -24 70.8 -13.2 -0.7 -6.6 ATATATATTTTAAACTGCCA

8531 5EQ ID N0:2775 -9.9 -17.3 54.3 -7.4 0 -4.8 TATATATATTTTAAACTGCC

8532 SEQ ID N0:2776 -9.9 -16.3 52.5 -6.4 0 -5.2 GAACATGTGAACTTGATCCT

8695 SEQ ID N0:2777 -9.9 -21 62.1 -11.1 0 -7 ACCTGAAAGTTGGAACATGT

8707 SEQ ID N0:2778 -9.9 -20.8 61.2 -10.1 -0.6 -6.7 AGTTTAGATGCTTATAGCCA

8782 SEQ ID N0:2779 -9.9 -22.4 67.5 -11.2 -1.2 -4.6 ATCATTAATACCTTATGTAT

8901 SEQ ID N0:2780 -9.9 -17.7 56 -7 -0.6 -4.2 ATATCATTAATACCTTATGT

8903 SEQ ID N0:2781 -9.9 -17.7 56 -7.8 0 -4 ATAATTTATTATGGGAGAAT

9066 SEQ ID N0:2782 -9.9 -15.6 51.1 -5.1 -0.3 -4.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTTTTTTTTTGACCACAGAG

100 5EQ ID N0:2783 -9.8 -19.9 61.1 -10.1 0 -2.3 GTGTCTTCCTCTGGCAGCTG

137 SEQ ID N0:2784 -9.8 -28.8 84.4 -18.5 0 -8.2 ATGTGGCTTGGCTTCAGTTT

241 SEQ ID N0:2785 -9.8 -25.7 76.4 -15.9 0.4 -4.4 AGATTCTTTGCTCCTTTCCC

316 SEQ ID N0:2786 -9.8 -26.8 77.1 -17 0 -3.6 GAAGCTTTCAGGTCCTGGGG

494 SEQ ID N0:2787 -9.8 -27.2 77.9 -16 -0.4 -10.8 CTTTCTCTTCTGCAGCACGT

552 SEQ ID N0:2788 -9.8 -26.3 76.6 -16 0 -8.3 GGGCTTCTTGGCTTTCTCTT

563 SEQ ID N0:2789 -9.8 -27.4 81.3 -16.6 -0.9 -3.7 ATAAATGGAAGGTTCTTTCC

643 SEQ ID N0:2790 -9.8 -19.8 60.2 -8.4 -1.6 -6.5 AGTGACTCAAAGGTATAGAT

961 SEQ ID N0:2791 -9.8 -19 59.4 -9.2 0 -3.1 CCAATGAGAGCAAACACGCT

1246 SEQ ID N0:2792 -9.8 -22.9 63.4 -12.2 -0.7 -4.7 TTATTCCTCAGATTGCCCAT

1279 5EQ ID N0:2793 -9.8 -25.5 72.2 -15.7 0 -3 TCTCTCTCTCCTTTGTTGTT

2014 SEQ ID N0:2794 -9.8 -25.3 77.9 -15.5 0 0 TGCAATCCACAGTGCTGTGC

2403 SEQ ID N0:2795 -9.8 -26.7 75.7 -14.7 -1.7 -12.4 TAACCTTCTCTTTCTGACTT

2510 SEQ ID N0:2796 -9.8 -22.1 66.7 -12.3 0 -2.6 CTTCCAGAGGAATCCTCCAG

2557 SEQ ID N0:2797 -9.8 -26.2 73.7 -14.2 -2.2 -11.2 CATAAAGAGGGTATTTAAGA

2783 SEQ ID N0:2798 -9.8 -16.7 53.4 -6.9 0 -3 TCCACATTTGACAGACCAAG

2986 SEQ ID N0:2799 -9.8 -22.5 64.7 -12.7 0 -2.9 GAAGTCGTTCATGTGCCACC

3239 SEQ ID N0:2800 -9.8 -26.6 74.6 -16.1 -0.4 -4.7 GGTTCAGAACCACAAGGTTT

3384 SEQ ID N0:2801 -9.8 -23.2 67.7 -10.8 -2.6 -11.3 ATAATCAATTCCCTTTTGCA

3503 SEQ ID N0:2802 -9.8 -21.6 63.1 -11.8 0 -4.8 CAGTTTCAGCTTGTTCACCT

3924 SEQ ID N0:2803 -9.8 -25.5 75.6 -15.7 0 -4.5 ATATCTTCAAAGGCCAATGC

4135 SEQ ID N0:2804 -9.8 -21.6 63.1 -11.2 -0.3 -7.7 ATAAGGCTCTTAGAGGTCTT

4398 SEQ ID N0:2805 -9.8 -22.4 68.7 -12 -0.3 -6.1 CGTTGTCATGTTAACACAGT

4559 SEQ ID N0:2806 -9.8 -21.7 64.9 -11.1 -0.5 -8.7 AGCGCCAACATTATCAAAGT

4667 SEQ ID N0:2807 -9.8 -22 62.8 -11.7 0 -7.6 TGGGCGAGGTATGGGTTTCT

4985 SEQ ID N0:2808 -9.8 -26.8 76.8 -17 0 -4 CTGGGCGAGGTATGGGTTTC

4986 SEQ ID N0:2809 -9.8 -26.8 76.8 -17 0 -4 ACCATTCCTTGGAATTTGTT

5008 SEQ ID N0:2810 -9.8 -23 66.8 -11.5 -1.7 -8.4 ATGTATTTGCCCTGGTCATC

5113 SEQ ID N0:2811 -9.8 -25.7 74.6 -15.9 0 -4~2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Tntra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ACGAGCTTCAGCACAAATTC

5185 SEQ TD N0:2812 -9.8 -22 64.2 -11.3 -0.7 -6.6 CCAGCCTATAGTGAAGTAGT

5219 SEQ ID N0:2813 -9.8 -24 70.5 -14.2 0 -4.6 CGGATCCCCTTTGCTCCTTT

5377 SEQ ID N0:2814 -9.8 -30.3 80.3 -19.8 -0.4 -7.5 GAGAACTCTATAAACTGGGT

5884 SEQ ID N0:2815 -9.8 -19.6 59.7 -9.8 0 -4.9 AGAGAACTCTATAAACTGGG

5885 SEQ ID N0:2816 -9.8 -18.4 57 -8.6 0 -6.4 AACTCCCATCTGTTTTTTCT

6336 SEQ ID N0:2817 -9.8 -23.8 70 -14 0 -3.1 TTGTCTTTCTCAAACTTTTC

6406 SEQ ID N0:2818 -9.8 -19.6 61.9 -9.8 0 -2.6 CCTTTGCTTTCTTTTTCTGG

6427 SEQ ID N0:2819 -9.8 -24.2 71.9 -14.4 0 -2.9 ATGAGTATTTGTTAAAACAG

6575 SEQ ID N0:2820 -9.8 -15.5 51.1 -5.7 0 -6.1 TTGGGTTTCTCCTCAGCAGT

6700 SEQ ID N0:2821 -9.8 -27.3 8l -16.6 -0.8 -4.9 TGACATAGCATTATGAATAA

6880 SEQ ID N0:2822 -9.8 -16.4 52.4 -5.1 -1.4 -4.9 TAGTGACATAGCATTATGAA

6883 SEQ TD N0:2823 -9.8 -18.3 57.3 -7 -1.4 -4.9 ATTAGATTACTTTTTGGAAA

7235 SEQ ID N0:2824 -9.8 -16.1 52.5 -6.3 0 -2.3 AATTATTCTGCTTGACATAA

7668 SEQ ID N0:2825 -9.8 -17.8 55.9 -8 0 -3.8 GAATAAAAGGTTATATTGAG

8138 SEQ ID N0:2826 -9.8 -14.1 47.9 -3.7 -0.3 -3 TGAATAAAAGGTTATATTGA

8139 SEQ ID N0:2827 -9.8 -14.1 47.8 -3.7 -0.3 -3 TGTTATATTTGTAATAGCTT

8237 SEQ ID N0:2828 -9.8 -17.6 56.8 -7.8 0 -7.1 TTTAGCAATAGCAATTCCCT

8407 SEQ ID N0:2829 -9.8 -22.6 65.4 -11.1 -1.7 -4.6 CTTGGTGAATCATGCACTAG

8618 SEQ TD N0:2830 -9.8 -21.7 64.8 -10.7 -1.1 -6.2 GAGTTTAGATGCTTATAGCC

8783 SEQ ID N0:2831 -9.8 -22.3 67.6 -11.2 -1.2 -4.6 GTTTATATAATGCATTTAAG

8873 SEQ TD N0:2832 -9.8 -15.5 51.3 -5 0 -8.8 AGTTTATATAATGCATTTAA

8874 SEQ ID N0:2833 -9.8 -15.5 51.3 -5 0 -8.8 CTAGTAAAAAGGCGGAAGCT

508 SEQ ID N0:2834 -9.7 -20.3 59.2 -8.2 -2.4 -6.9 ACGTTTTTCGATAGCAGCAA

536 SEQ ID N0:2835 -9.7 -22.4 64.7 -11.7 -0.9 -6.9 CAAGATTTTTATAAGTGACT

974 SEQ ID N0:2836 -9.7 -16.6 53.6 -6.9 0 -4.1 GCTCGGAGAACTCTGAATGT

1120 SEQ ID N0:2837 -9.7 -23.1 66.9 -12.7 -0.4 -8.2 TATTCCTCAGATTGCCCATG

1278 SEQ ID N0:2838 -9.7 -25.4 71.7 -15.7 0 -3.9 ACATTTATTCCTCAGATTGC

1283 SEQ ID N0:2839 -9.7 -21.8 65.8 -12.1 0 -2.6 TTTCCATCCATGGAGAAAAG

2083 SEQ ID N0:2840 -9.7 -20.7 60.6 -9.1 -1.7 -11.5 kcal/

mol kcal/mo1deg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGTTTCCATCCATGGAGAAA

2085 SEQ ID N0:2841 -9.7 -22.6 65.3 -10.8 -1.7 -12.1 AAACAGGGAGCCACGGATAC

2153 SEQ ID N0:2842 -9.7 -23.7 65.8 -14 0.5 -4.3 TTGCGTCGCTCTCCATGTCT

2317 SEQ ID N0:2843 -9.7 -28.7 80.2 -18.4 -0.3 -6.9 TTTGCTGGAAGCCCTGGCAC

2374 SEQ ID N0:2844 -9.7 -28.6 77.9 -16.7 -2.2 -8.6 ACCATTGCAATCCACAGTGC

2408 SEQ ID N0:2845 -9.7 -25.8 72.1 -15.2 -0.7 -9 GGGGGAAGTTGTCCAGTAGG

2467 SEQ TD N0:2846 -9.7 -26.4 76.8 -14.7 -2 -5.5 TTTGGCCAGCGACCTCCATA

3324 SEQ TD N0:2847 -9.7 -29 77.4 -18.6 -0.1 -8.8 TAGCAGCAAGGTTGTCTGAG

3435 SEQ TD N0:2848 -9.7 -23.6 70.9 -12.5 -l.3 -8.2 GTTGTTTATGAATGACATAT

3740 SEQ ID N0:2849 -9.7 -17.5 55.8 -6.7 -1 -6 TTGGCACTGTGACGGTGAGG

3765 SEQ ID N0:2850 -9.7 -25.9 73.3 -14.4 -1.8 -7.6 ACTGTAGCAGGTTTTTCGAA

4055 SEQ ID N0:2851 -9.7 -22.4 66.7 -11.8 -0.7 -7.5 AACATCAACGATCAAGAAAT

4298 SEQ ID N0:2852 -9.7 -15.8 50 -6.1 0 -4.9 AAGAAACATCAACGATCAAG

4302 SEQ ID N0:2853 -9.7 -15.8 50.2 -6.1 0 -4.9 ATTGCATTGTAATATTTTTT

4939 SEQ ID N0:2854 -9.7 -17.1 55 -7.4 0 -5.2 GGTCATCCGTTTCCACCATC

5100 SEQ ID N0:2855 -9.7 -28.5 79 -18.1 -0.4 -4.1 CAGATCATGCTGTTGCCAAA

5554 SEQ TD N0:2856 -9.7 -23.5 67.1 -12.9 -0.8 -6.6 CCTTAACTGAGCTGCCAGGG

5667 SEQ ID N0:2857 -9.7 -27.3 75.1 -16.7 -0.6 -9.3 TGTTCACCACAACCAGGAAG

5745 SEQ ID N0:2858 -9.7 -23.5 66.5 -13.8 0 -5.5 GGACTTTGTTGGGTTTTGCT

5949 SEQ ID N0:2859 -9.7 -25 74.2 -15.3 0 -3.6 GCGGCAGACACCTCCTCTTG

6166 SEQ ID NO:2860 -9.7 -29.6 80.2 -19.4 -0.1 -5.9 AGCGGCAGACACCTCCTCTT

6167 SEQ ID N0:2861 -9.7 -29.6 80.8 -19.4 -0.1 -6.6 TGTTTTTTCTGGAGTGGAGT

6326 SEQ ID N0:2862 -9,7 -23 71 -13.3 0 -2.7 CTATGAGTATTTGTTAAAAC

6577 SEQ ID N0:2863 -9.7 -15.4 51 -5.7 0 -3.3 GCACTGACTATGAGTATTTG

6584 SEQ TD N0:2864 -9.7 -20.7 63.3 -10.5 -0.1 -3.5 TTGTATAGGCACTGACTATG

6592 SEQ ID N0:2865 -9.7 -20.9 63.6 -10.5 -0.4 -4.8 GGTAGCCATTGGGTTTCTCC

6708 SEQ ID N0:2866 -9.7 -28.2 81.3 -18 -0.2 -5.6 CTGTACTAAAGGTGTTTGGT

6770 SEQ ID N0:2867 -9.7 -21.4 65.2 -10.9 -0.6 -5.1 AGTTAAAAATAGAATGGATG

6796 SEQ ID N0:2868 -9.7 -13.6 46.6 -3.9 0 -2.8 TTCTTGTATACTGTTTCTTC

6926 SEQ ID N0:2869 -9.7 -20.4 65.4 -10.7 0 -5.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GCACATAACTGTTCAGATAG

7101 SEQ ID N0:2870 -9.7 -20.2 61.8 -l0 -0.2 -5.6 TTATTTTAGGAAATTATCCA

8002 5EQ ID N0:2871 -9.7 -17.1 54.3 -5.2 -2.2 -7.2 AAACCCATTATCTAACTTTT

8020 SEQ ID N0:2872 -9.7 -19 57.3 -9.3 0 -1.6 TCCATGTTATATTTGTAATA

8241 SEQ TD N0:2873 -9.7 -17.9 56.7 -8.2 0 -4.3 TACACTACAGTAAATAGTTT

8371 SEQ ID N0:2874 -9.7 -16.9 54.6 -6.7 -0.1 -3.3 TTTATTAGCCACCAAGTAAT

8836 SEQ ID N0:2875 -9.7 -20.6 61.1 -10.9 0 -3.2 TGTCTTCCTCTGGCAGCTGT

136 SEQ ID N0:2876 -9.6 -28.8 84.4 -18.5 0 -8.7 GGCTTGGCTTCAGTTTTCTT

237 SEQ ID N0:2877 -9.6 -26 78 -15.7 -0.4 -6 CACTATTTGGCTTTGGTTTG

612 SEQ ID N0:2878 -9.6 -22.4 67.1 -12.8 0 -3.7 GAAGGTTCTTTCCAGCTTCC

636 SEQ ID N0:2879 -9.6 -26.2 76.3 -15.9 -0.5 -4.7 CCTTTCCTTTATTCATTACT

744 SEQ ID N0:2880 -9.6 -22.4 66.7 -12.8 0 -0.7 AATACACAGTTGGTCAAAAT

883 SEQ ID N0:2881 -9.6 -17.5 54.6 -7.9 0 -3.8 TTTTTATAAGTGACTCAAAG

969 SEQ TD N0:2882 -9.6 -15.7 51.7 -6.1 0 -4.7 GCTTCTTTACCGACTGGATC

1191 SEQ ID N0:2883 -9.6 -24.9 71.5 -14.4 -0.7 -4.1 GAATCGCTTGGGGGCCACTG

1306 SEQ ID N0:2884 -9.6 -28.3 76.1 -18 -0.4 -6.8 CCCATTTGAATCCATTGTGC

1361 SEQ ID N0:2885 -9.6 -25 69.7 -15.4 0 -2.6 GAACTCAACTTTGATGCTTC

1930 SEQ ID N0:2886 -9.6 -20.5 62.1 -10.9 0 -5 TTCCTCCATTCTTTAGCACC

1954 SEQ ID N0:2887 -9.6 -26.5 75.8 -16.9 0 -4.1 TTGCTGGAAGCCCTGGCACC

2373 SEQ ID N0:2888 -9.6 -30.5 80.9 -18.7 -2.2 -8.7 GGAAGTTGTCCAGTAGGTGA

2464 SEQ ID N0:2889 -9.6 -24.6 73.4 -13.7 -1.2 -4.8 TAAGCAGTCTGAATGATCGC

3021 SEQ ID N0:2890 -9.6 -21.5 63.6 -11.2 -0.4 -6.3 GTTTCCTAGAGCCCCCACAG

3104 SEQ ID N0:2891 -9.6 -30.4 82.1 -20.8 0 -3.3 ACCTCCATACAGTCCCACAT

3313 SEQ ID N0:2892 -9.6 -28 76.5 -18.4 0 -2.8 TCCCTTTTGCATTCTTCCTA

3494 SEQ ID N0:2893 -9.6 -26.6 75.9 -17 0 -5.1 ATCTCTAAGATAATTAAGCT

3650 5EQ ID N0:2894 -9.6 -17 54.6 -6.8 -0.3 -5.4 TTTGCTTTCTTCTAGTTCTG

3842 SEQ ID N0:2895 -9.6 -22.1 69.4 -12.5 0 -4 TTCTTCGGGTTCAGTTTCAG

3935 SEQ ID N0:2896 -9.6 -24 72.8 -14.4 0 -2.4 TCTGTACTTACTTGACAGAA

4006 SEQ ID N0:2897 -9.6 -19.7 61 -8.2 -1.9 -6.3 TTTGCCTTCTTCTGTACTTA

4016 SEQ ID N0:2898 -9.6 -23.5 70.9 -13.9 0 -4.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATCTTCCCTTTGCCTTCTTC

4024 SEQ ID N0:2899 -9.6 -27.5 79.2 -17.9 0 -3 CAACAATACTGTAGCAGGTT

4062 SEQ ID N0:2900 -9.6 -20.9 62.7 -10.4 -0.7 -5.3 GCATTCACAACCACCCTCAT

4432 SEQ ID N0:2901 -9.6 ~ -27 73.3 -17.4 0 -3.4 TTACTTTCACGTTTTTCCAC

4647 SEQ ID NO:2902 -9.6 -22.1 65.8 -12.5 0 -4.7 ACATGTACAGATTTTCTTCA

4785 SEQ ID N0:2903 -9.6 -20.1 62.6 -10.5 0 -6.7 TTCCACCATCATGGTGACCA

5090 SEQ ID N0:2904 -9.6 -27.1 74.7 -13.8 -3.7 -11.1 TGGTCATCCGTTTCCACCAT

5101 SEQ ID N0:2905 -9.6 -28.1 77.1 -17.3 -1.1 -5.4 TTCAGCACAAATTCTCCAGT

5179 SEQ ID N0:2906 -9.6 -22.9 67.4 -13.3 0 -4.1 TTTTCTATCATCTCAGCCAG

5287 SEQ ID N0:2907 -9.6 -23.3 70.4 -13.7 0 -3.2 GGATCCCCTTTGCTCCTTTG

5376 SEQ ID N0:2908 -9.6 -29.5 80.5 -19.9 0 -6.9 CAGGAGGCCGATGTTAAACA

5435 SEQ ID N0:2909 -9.6 -23.4 65.6 -13.2 -0.3 -6.9 AAAGATGGCATAGATAAACA

5468 SEQ ID N0:2910 -9.6 -16.4 51.9 -6.8 0 -4 CCATCCCAGCCAGCAGAGGT

5590 SEQ ID N0:2911 -9.6 -31.8 85.1 -22.2 0 -4.6 AGCTGGACTTTGTTGGGTTT

5953 SEQ ID N0:2912 -9.6 -24.9 74.1 -15.3 0 -4.3 GGTCACCACTGACCATGGGC

5988 SEQ ID N0:2913 -9.6 -29.2 80 -15.7 -3.9 -10.6 TATATTTTTTAACCTTTGCT

6221 SEQ ID NO:2914 -9.6 -18.9 58.6 -9.3 0 -3.6 TGGAGTGGAGTTCCCATTTA

6317 SEQ ID N0:2915 -9.6 -25.1 73.1 -14.6 -0.7 -6.7 GACCTCTTTTCCTTTGCTTT

6437 SEQ ID N0:2916 -9.6 -25.7 74.8 -16.1 0 -3.6 ACACATATACTTTACCTTCA

6519 SEQ TD N0:2917 -9.6 -20.5 62 -10.9 0 -2.2 CACTGACTATGAGTATTTGT

6583 SEQ ID N0:2918 -9.6 -20.1 62.2 -10.5 0.5 -4 GGTCACTTCACTGTCTTGTA

6607 SEQ ID N0:2919 -9.6 -24.4 74.7 -14.8 0 -2.6 GTTAAAAATAGAATGGATGC

6795 SEQ ID N0:2920 -9.6 -15.4 50.1 -5.8 0 -2.8 AAATATGGCAGATATGGAAG

6814 SEQ ID N0:2921 -9.6 -17.4 54.2 -6.4 -1.3 -5.2 AATTGGGGACCATGGAAATG

6854 SEQ ID N0:2922 -9.6 -20.9 60.2 -8.9 -2.4 -8.8 CATTACAATTCTTTTTCTGG

7008 SEQ ID N0:2923 -9.6 -19 59.3 -9.4 0 -2.5 AAGCCATCTAGAAAATGGAA

7045 SEQ ID N0:2924 -9.6 -18.4 55.5 -7.2 -1.6 -8.3 AGAATGGATTTATTAGATTA

7246 SEQ ID N0:2925 -9.6 -15.8 52.1 -6.2 0 -2.4 TTTGATTTAATAGAAGTTGT

7447 SEQ ID N0:2926 -9.6 -16.1 53 -6.5 0 -2.5 ATAATGGACAGAGCAGGTTG

7652 SEQ ID N0:2927 -9.6 -21.3 63.9 -11.7 0 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GCTTCAAATTATTCTGCTTG

7674 SEQ ID N0:2928 -9.6 -20.5 62.3 -10.9 0 -3.6 TATTTTAGGAAATTATCCAT

8001 SEQ ID N0:2929 -9.6 -17 54 -5.2 -2.2 -7.2 ATACACTACAGTAAATAGTT

8372 5EQ ID N0:2930 -9.6 -16.8 54.2 -6.7 -0.1 -3.6 ACGTGTCTTCCTCTGGCAGC

139 SEQ ID N0:2931 -9.5 -28.9 82.6 -19.4 0 -4.4 TGGCTTGGCTTCAGTTTTCT

238 SEQ ID N0:2932 -9.5 -25.9 77.3 -15.7 -0.4 -6 TTTTCCGGAAAAGCTCCAGG

382 SEQ ID N0:2933 -9.5 -24.2 67.1 -11.9 -1.6 -13.8 CTTCTGCAGCACGTTTTTCG

546 SEQ ID N0:2934 -9.5 -25.1 71.5 -14.7 -0.5 -9.3 AAGTCACTATTTGGCTTTGG

616 SEQ ID N0:2935 -9.5 -21.9 66 -11 -1.3 -4.7 AGTCAGGAGGGTTGCTCAAG

909 SEQ ID N0:2936 -9.5 -24.9 74.4 -13.4 -2 -4.6 ATTGCCCAGGTCCACAAACT

1085 SEQ ID N0:2937 -9.5 -26.7 72.6 -17.2 0 -3.6 TCTCAACGCTGAGACATTGC

1100 SEQ ID N0:2938 -9.5 -23.4 67.5 -11.4 -2.5 -8.4 CTCGGAGAACTCTGAATGTT

1119 SEQ ID N0:2939 -9.5 -21.4 63.2 -11.2 -0.4 -8.2 CCTCAGATTGCCCATGAACA

1274 SEQ ID N0:2940 -9.5 -26 71.1 -16.5 0 -4.5 CATAAAAGTGACTGTCATCT

1431 SEQ ID N0:2941 -9.5 -18.8 58.2 -8.6 -0.1 -8.8 GCTGATGCTGCCGCAACTGC

1852 SEQ TD N0:2942 -9.5 -29.3 78.2 -18.6 -1.1 -7.5 ATTTGCTGGAAGCCCTGGCA

2375 SEQ ID N0:2943 -9.5 -28.4 77.3 -16.7 -2.2 -8.6 TTGCTCAGTCATGGGGTAGT

2813 SEQ ID N0:2944 -9.5 -25.9 77.9 -16.4 0 -4.7 ACACACTACTGAATTGCTCA

2826 SEQ ID N0:2945 -9.5 -21.5 63.8 -12 0 -3.6 GAGGTTTCCTAGAGCCCCCA

3107 SEQ ID N0:2946 -9.5 -31.3 84.3 -21.1 -0.5 -4.4 GCAGCAAGGTTGTCTGAGCT

3433 SEQ ID N0:2947 -9.5 -26.6 77.9 -15.7 -1.3 -8.2 CATAATCAATTCCCTTTTGC

3504 SEQ ID N0:2948 -9.5 -21.6 63.1 -12.1 0 -2.7 GCATCTTATTTTTCACATAA

3519 SEQ ID N0:2949 -9.5 -19 59.3 -9.5 0 -3.4 GCTTTCTAAAAAAGGCTTTT

3552 SEQ ID N0:2950 -9.5 -18.6 57 -7.6 -1.4 -7.2 CTAAGATAATTAAGCTCTTT

3646 SEQ ID N0:2951 -9.5 -16.8 54 -6.8 -0.1 -6.1 TCCAACAGCAATTGGCACTG

3776 SEQ ID N0:2952 -9.5 -24 67.6 -12.9 -1.5 -8.l TGATTTGATGGCACCGAGTT

4358 SEQ ID N0:2953 -9.5 -24 68.2 -14.5 0 -4 CGGGATAAGGCTCTTAGAGG

4402 SEQ ID N0:2954 -9.5 -23.6 68.2 -13.5 -0.3 -5.6 GTGGTAGAACTTGCCAGCAA

4541 SEQ ID N0:2955 -9.5 -24.7 70.6 -13.9 -1.2 -7.3 CCACCGAGCTTGCTTGCCAA

4631 SEQ ID N0:2956 -9.5 -29.7 77.2 -19.6 -0.3 -6.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATATCCATCCAGCCTTTAAA

4717 SEQ ID N0:2957 -9.5 -23.1 65.8 -13.6 0 -4 TCCACCATCATGGTGACCAT

5089 SEQ ID N0:2958 -9.5 -27 74.3 -13.8 -3.7 -11.1 ACTAGGGTCATGTATTTGCC

5122 SEQ ID N0:2959 -9.5 -24.4 72.3 -14.9 0 -4.7 CAAAGGTCTCAAAGTTGAAC

5538 SEQ ID N0:2960 -9.5 -18.1 56.1 -8.6 0 -5.4 AATCCCAACAGATGGGTCCC

5696 SEQ ID N0:2961 -9.5 -27.2 73.4 -14.5 -3.2 -8.6 TTCACCACAACCAGGAAGGA

5743 SEQ ID N0:2962 -9.5 -24.1 67.3 -13.8 -0.6 -4.3 GGATCAAACTTTTCCCAAAC

5857 SEQ ID N0:2963 -9.5 -20.8 60.4 -11.3 0 -5.3 ACCACTGACCATGGGCAGAT

5984 SEQ ID N0:2964 -9.5 -27 74 -15.7 -1.8 -8.8 TCCATCTCTCCACTCTCACA

6055 SEQ ID N0:2965 -9.5 -27.2 78.5 -17.7 0 -0.5 CAAACTTTTCCTTGTCTGGT

6396 SEQ ID N0:2966 -9.5 -23 67.8 -13.5 0 -3.2 CTGACCTCTTTTCCTTTGCT

6439 SEQ ID N0:2967 -9.5 -26.4 75.8 -16.9 0 -3.6 GTCCAGTTGACACATATACT

6528 SEQ ID N0:2968 -9.5 -22.5 67.2 -11.9 -1 -4.4 TTAAAAATAGAATGGATGCA

6794 SEQ ID N0:2969 -9.5 -14.9 48.7 -5.4 0 -5.2 TTGTATACTGTTTCTTCAAC

6923 SEQ TD N0:2970 -9.5 -19.2 60.9 -9.7 0 -6.3 AAAAATAATCCTTTAAATAT

7138 SEQ ID N0:2971 -9.5 -11.7 42.7 -2.2 0 -4.3 TTTTGGAAAGCATTTGACCT

7224 SEQ ID N0:2972 -9.5 -21.1 62.1 -10.8 -0.6 -4.1 ACATCCCTGTTGGAAGCTTG

7494 SEQ ID N0:2973 -9.5 -25.4 71.9 -14.8 -1 -7.2 TGTCAGTAGGCAGTATCCAG

7775 SEQ ID N0:2974 -9.5 -24.9 75 -14.9 -0.2 -4 TAGGAAATTATCCATTGAAA

7996 SEQ ID N0:2975 -9.5 -16.3 51.7 -4.6 -2.2 -7.2 GAGTGTTTGACCAATGTATC

8085 SEQ ID N0:2976 -9.5 -21.3 64.7 -11.8 0 -3.4 AAAGAACAAAGTCCATGTTA

8252 SEQ ID N0:2977 -9.5 -17.3 53.8 -6.5 -1.2 -4.7 ACAACTTTGCCACTTTGTTC

8280 SEQ ID N0:2978 -9.5 -23 67.7 -13.5 0 -3.8 TATATCATGTTAGGTAATTG

8301 SEQ ID N0:2979 -9.5 -17 55.2 -7.5 0 -4.1 CACTACAGTAAATAGTTTTG

8369 SEQ ID N0:2980 -9.5 -17.1 54.9 -7.1 -0.1 -3.3 ATGCAATAAAAAATGAAATT

8466 SEQ ID N0:2981 -9.5 -11.3 41.7 -1.8 0 -5.6 AATGCAATAAAAAATGAAAT

8467 SEQ ID N0:2982 -9.5 -10.5 40.3 -0.9 0 -5.6 CACTAGTTTGTTGTAAAATT

8604 SEQ ID N0:2983 -9.5 -17.1 54.8 -6.9 -0.4 -6.3 CAAACCTGAAAGTTGGAACA

8710 SEQ ID N0:2984 -9.5 -18.9 56.1 -8.7 -0.4 -4.8 TATGTATACATAGGAGTTTA

ggg8 SEQ ID N0:2985 -9.5 -17.8 57.4 -7.1 0 -10.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CCTCTCTGCTTATGCCTCCG

8 SEQ ID N0:2986 -9.4 -29.9 81 -19.6 -0.8 -4.6 TTCTTACAATATCCCTAGAA

337 SEQ ID N0:2987 -9.4 -20 60.2 -10.6 0 -3.8 AGCTCCAGGTCCCTTCTGTG

371 SEQ ID N0:2988 -9.4 -30.1 85.5 -20.7 0.3 -6.3 CTTTCAGGTCCTGGGGGTAC

490 SEQ ID N0:2989 -9.4 -27.8 80.8 -17.7 -0.4 -6.8 GAATCGGAAAATTGCCTTTC

758 SEQ ID N0:2990 -9.4 -20.2 59 -10.3 -0.2 -3.9 CAGTTGGTCAAAATAGTGCA

877 SEQ ID N0:2991 -9.4 -20.8 62.6 -11.4 0 -5.2 ACACAGTTGGTCAAAATAGT

880 SEQ ID N0:2992 -9.4 -19.4 59.6 -l0 0 -3.8 CAGCCCAATGAGAGCAAACA

1250 SEQ ID N0:2993 -9.4 -23.7 65.5 -13.5 -0.6 -4.l GGGGGCCACTGCAAACATTT

1297 SEQ ID N0:2994 -9.4 -26.6 72.5 -15.7 -1.4 -7.7 AAAAATATCATGTATGTTTT

1663 SEQ ID N0:2995 -9.4 -14.4 48.6 -4 -0.9 -7.5 CCATGGCCACCACAGCCAGG

1728 SEQ ID N0:2996 -9.4 -31.9 82.3 -19.7 -2.8 -9.1 CTTCAAGGTGCTCTCTCTGT

1992 SEQ ID N0:2997 -9.4 -25.6 77.2 -16.2 0 -3.6 TGCCCGACCTCTGAAACTGA

2204 SEQ ID N0:2998 -9.4 -26.5 70.5 -17.1 0 -3 AGTCTCTCCTGCTTTCGCCG

2280 SEQ TD N0:2999 -9.4 -29.8 82 -19.7 -0.5 -3.6 ATTCACAAGATGTTTTACTT

2714 SEQ ID N0:3000 -9.4 -18.4 58.1 -9 0 -3 AATTGCCAATGATCTTAATT

3084 SEQ ID N0:3001 -9.4 -18.3 56 -8.9 0 -4.9 AAGACGATGATGGCCAACAC

3133 SEQ TD N0:3002 -9.4 -22.2 62.5 -12.8 0 -6.3 AGTCCCACATGGTCTCTATC

3303 SEQ ID N0:3003 -9.4 -26.3 77.4 -16.9 0 -5.2 CAGAACCACAAGGTTTCCAA

3380 SEQ ID N0:3004 -9.4 -22.7 64.4 -10.7 -2.6 -7.6 AGCAGCAAGGTTGTCTGAGC

3434 SEQ ID N0:3005 -9.4 -25.7 76.2 -14.9 -1.3 -8.2 ATTGGCACTGTGACGGTGAG

3766 SEQ ID N0:3006 -9.4 -24.7 70.7 -14.1 -1.1 -6.9 TGCTTTCTTCTAGTTCTGAC

3840 SEQ ID N0:3007 -9.4 -22.7 70.7 -13.3 0 -4 GCATTTAATTTCTCTTTGCT

3856 5EQ ID N0:3008 -9.4 -21.4 65.3 -11.5 -0.2 -4 CCCTTCAGTAAAACAAGCTT

3968 SEQ ID N0:3009 -9.4 -21.9 63 -12.5 0 -6.2 TTCTTCTGTACTTACTTGAC

4010 SEQ ID N0:3010 -9.4 -20.6 64.7 -11.2 0 -4.8 ATGATTTGATGGCACCGAGT

4359 SEQ ID N0:3011 -9.4 -23.9 67.9 -14.5 0 -4 ATTCACAACCACCCTCATGC

4430 SEQ ID N0:3012 -9.4 -26.3 72.1 -16.9 0 -4.4 CTTGCCAGCAAACAAATTCA

4532 SEQ ID N0:3013 -9.4 -21.3 61.1 -11.9 0 -7.4 TTTTCCACCGAGCTTGCTTG

4635 SEQ ID N0:3014 -9.4 -26.6 73.8 -16.7 -0.2 -6.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TACATAATATCCATCCAGCC

4723 SEQ ID N0:3015 -9.4 -23.3 66.5 -13.9 0 -3.2 CTTCATATACAGGCTGAAGT

4770 SEQ ID N0:3016 -9.4 -21.4 64.6 -10.5 -1.4 -5.9 GTTTCTTGGATCCAAGTTTC

4962 SEQ ID N0:3017 -9.4 -22.8 69.3 -9.7 -1.3 -15.5 GAGGTTTCTTGGATCCAAGT

4965 SEQ ID N0:3018 -9.4 -24 71.3 -10.9 -1.3 -15.5 AATTCTCCAGTGAACAGAAC

5170 SEQ ID N0:3019 -9.4 -19.9 60 -10 -0.2 -4.8 GAATACTTTTCTATCATCTC

5293 SEQ ID N0:3020 -9.4 -18.8 60 -9.4 0 -2.6 ATAGGCAAAGTTGGACATCC

5489 SEQ ID N0:3021 -9.4 -22.4 65.5 -13 0 -4.5 GTCATCAATTCCAGCTTCCT

5516 SEQ ID N0:3022 -9.4 -26 75.1 -16.6 0 -4.5 TCACCACAACCAGGAAGGAT

5742 SEQ ID N0:3023 -9.4 -24 66.9 -13.8 -0.6 -4.3 ATGACCGCGATGTACATGTT

5761 SEQ ID N0:3024 -9.4 -23.9 66.8 -13.8 0 -8.9 AGTTTGGCATGGACCTCCTC

6553 SEQ ID N0:3025 -9.4 -27.7 78.9 -18.3 0.1 -5.1 AAATAGAATGGATGCAAGGA

6790 SEQ ID N0:3026 -9.4 -17.6 54.2 -8.2 0 -5.6 ATAGTGACATAGCATTATGA

6884 SEQ TD N0:3027 -9.4 -19 59.3 -8.1 -l.4 -4.9 CTTCAACGTAAACCTCATTT

6910 SEQ ID N0:3028 -9.4 -20.3 59.7 -10.9 0 -4.8 TGAGAGACAGGGTTCTTGTA

6938 5EQ ID N0:3029 -9.4 -22.7 69.4 -12.6 -0.4 -5 ATAACTGTTCAGATAGAAAC

7097 SEQ ID N0:3030 -9.4 -16.2 52.6 -6.1 -0.5 -5.6 GGAAGCTTGCAAAAGACACA

7483 SEQ ID N0:3031 -9.4 -20.7 60.4 -9.8 -1.4 -7 ATTCTTTAACCAAGACTTGT

7606 SEQ ID N0:3032 -9.4 -19.8 60.3 -9.4 -0.9 -5.4 AATTCATTCTATCACCAATA

7632 SEQ ID N0:3033 -9.4 -18.6 57.4 -9.2 0 -2.3 TATGAAGAATAGGTTTTGTC

7791 SEQ ID N0:3034 -9.4 -17.5 56.3 -8.1 0 -1.9 TTAGACATATTTTTAGCAAA

7812 SEQ ID N0:3035 -9.4 -16.2 52.6 -6.8 0 -4.1 CTTTTATTTTAGGAAATTAT

8005 SEQ ID N0:3036 -9.4 -15.1 50.5 -5.1 -0.3 -4.6 ACTTTTATTTTAGGAAATTA

8006 SEQ ID N0:3037 -9.4 -15.3 51 -5.1 -0.6 -4.6 AAATGCAATAAAAAATGAAA

8468 SEQ ID N0:3038 -9.4 -9.8 39.1 0 0 -5.6 GTTGGAACATGTGAACTTGA

g6g9 SEQ ID N0:3039 -9.4 -20.2 60.9 -10 -0.6 -7 TCAAACCTGAAAGTTGGAAC

8711 SEQ ID N0:3040 -9.4 -18.6 56.1 -8.7 -O.l -4.6 AAAGCTATTGTAGGTGGTTA

8743 SEQ ID N0:3041 -9.4 -20.6 63.3 -11.2 0 -5.1 CTCACATAAATCCAAGGGTT

8958 SEQ ID N0:3042 -9.4 -21.3 62.4 -11.9 0 -4.1 GTTTGGTTTTGACCTCACAT

8971 SEQ ID N0:3043 -9.4 -23.8 70.4 -12.1 -2.3 -6.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TATAATTTATTATGGGAGAA

9067 SEQ ID N0:3044 -9.4 -15.3 50.6 -5.1 -0.6 -4.9 AATCCTTGACAGGTATCTCA

277 5EQ ID N0:3045 -9.3 -22.7 67.6 -12.4 -0.9 -3.4 TTGCTCCTTTCCCAGTAAGC

309 SEQ ID N0:3046 -9.3 -27.4 77.5 -17.4 -0.4 -4.1 TCACTATTTGGCTTTGGTTT

613 SEQ ID N0:3047 -9.3 -22.8 68.9 -13.5 0 -3.7 ACCAAAATCTTGATAGCAAT

829 SEQ ID N0:3048 -9.3 -18.3 55.5 -8.4 -0.3 -0.5 CCAGTCAGGAGGGTTGCTCA

911 SEQ ID N0:3049 -9.3 -28.3 81.8 -l7 -2 -5.1 CATTGCCCAGGTCCACAAAC

1086 SEQ ID N0:3050 -9.3 -26.5 71.8 -17.2 0 -3.6 CGGAGAACTCTGAATGTTCT

1117 SEQ ID N0:3051 -9.3 -21.4 63.2 -9.8 -2.3 -8.4 GCTGCAGCCCAATGAGAGCA

1254 SEQ TD N0:3052 -9.3 -28.7 78 -17.4 -1 -12 GAGCCATTTCCACAGAGTAA

1474 SEQ ID N0:3053 -9.3 -24.1 69.5 -14.8 0 -3.2 GACACTGGCCTGCATCTGAG

1491 5EQ ID N0:3054 -9.3 -26.9 75.9 -17.6 0 -7.2 CGAGCATCTGCTGAAATTCG

1800 SEQ ID N0:3055 -9.3 -22.4 63.5 -12.1 -0.9 -7.9 ACTTTGATGCTTCTGAAGAA

1923 SEQ ID N0:3056 -9.3 -19.8 60.4 -10 0 -8.3 ACCATGCATCACAGCAGTCC

2691 SEQ ID N0:3057 -9.3 -27.5 77.4 -16.9 -1.2 -7.5 AATTGCTCAGTCATGGGGTA

2815 SEQ ID NO:3058 -9.3 -24 71.2 -14.7 0 -4.7 TGAATTGCTCAGTCATGGGG

2817 SEQ ID N0:3059 -9.3 -23.7 69.6 -14.4 0 -4.7 CCACATTTGACAGACCAAGC

2985 SEQ ID N0:3060 -9.3 -23.9 67.2 -14.6 0 -2.9 GTCCCACATGGTCTCTATCC

3302 SEQ ID N0:3061 -9.3 -28.3 80.8 -19 0 -5.2 CCTCCATACAGTCCCACATG

3312 SEQ ID NO:3062 -9.3 -27.8 75.8 -18.5 0 -4.7 ACATAATCAATTCCCTTTTG

3505 SEQ ID N0:3063 -g.3 -20 59.7 -10.7 0 -2.6 CATCTCTAAGATAATTAAGC

3651 SEQ ID N0:3064 -9.3 -16.8 54 -6.8 -0.5 -4.9 CTCGGGGTAGAACAACATCA

3903 SEQ ID N0:3065 -9.3 -22.7 65.2 -13.4 0 -3.5 TCAGTTTCAGCTTGTTCACC

3925 SEQ ID N0:3066 -9.3 -25 75.4 -15.7 0 -4.5 ACCAACAGCACATTCATGAT

4474 SEQ ID N0:3067 -9.3 -22.3 64.4 -13 0 -6.4 TCCATCCAGCCTTTAAATGT

4714 SEQ ID N0:3068 -9.3 -24.6 69.3 -15.3 0 -4.3 CCTCTGTCATAAAGATGTCT

4914 SEQ ID N0:3069 -9.3 -21.9 65.7 -11.9 -0.4 -4.5 CTTCAGCACAAATTCTCCAG

5180 SEQ ID N0:3070 -9.3 -22.6 66.1 -13.3 0 -3.3 AACAGATGGGTCCCCACGGT

5690 SEQ ID N0:3071 -g,3 -28.9 76.8 -18.7 -0.6 -8.8 GTACATGTTCACCACAACCA

5750 5EQ ID N0:3072 -g,3 -24.2 68.6 -14.9 0.1 -6.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CCATCTCTCCACTCTCACAC

6054 SEQ TD N0:3073 -9.3 -27 77.3 -17.7 0 -0.5 GCATCCATCTCTCCACTCTC

6058 SEQ ID N0:3074 -9.3 -28.1 81.4 -18.8 0 -3.4 TTATAGGTAAGTCAATCCTC

6273 SEQ ID N0:3075 -9.3 -20.2 62.6 -10.4 -0.2 -3.5 TCTGGTTTTGTTACACTATC

6382 SEQ ID N0:3076 -9.3 -21.1 65.9 -11.8 0 -2.7 CCTTGTCTGGTTTTGTTACA

6387 SEQ ID N0:3077 -9.3 -24.1 72 -14.8 0 -2.7 TTACAATTCTTTTTCTGGTT

7006 SEQ ID N0:3078 -9.3 -19.6 61.5 -10.3 0 -2.6 TGACCTAAATTAAATATAGA

7210 SEQ ID N0:3079 -9.3 -14.4 48 -5.1 0 -3.2 ATTTATTAGATTACTTTTTG

7239 SEQ TD N0:3080 -9.3 -15.6 52.3 -6.3 0 -2.4 TAGAATGGATTTATTAGATT

7247 SEQ ID N0:3081 -9.3 -15.8 52.1 -6.5 0 -2.4 ACATAGGGTATTTAATTGAC

7365 SEQ ID N0:3082 -9.3 -18 56.9 -8.7 0 -2.9 TAGCCTTTAAACTATGTTTA

7530 SEQ ID N0:3083 -9.3 -18.8 58.3 -8.5 -0.9 -5.7 AGGCAATATTAACATGCATT

7555 SEQ ID N0:3084 -9.3 -19.3 58.4 -8 -2 -6.8 AGCAGGTTGAATTCATTCTA

7641 SEQ ID N0:3085 -9.3 -21.2 64.9 -11.2 -0.4 -8.4 TGTTGAATTAAAAGTGCAAA

7714 5EQ TD N0:3086 -9.3 -15.2 49.5 -5.9 0 -5.4 TCCAGTGTGTTTCCTTGAAA

7760 SEQ TD N0:3087 -9.3 -23.5 68.8 -13.5 -0.4 -4.5 GCAGTATCCAGTGTGTTTCC

7766 SEQ ID N0:3088 -9.3 -26.7 79.3 -17.4 0 -3.7 ATTTTAGGAAATTATCCATT

8000 SEQ ID N0:3089 -9.3 -17.4 54.9 -5.9 -2.2 -7.2 ATTATCTAACTTTTATTTTA

8014 SEQ ID N0:3090 -9.3 -15.2 51.3 -5.9 0 -l.6 AATACACTACAGTAAATAGT

8373 SEQ ID N0:3091 -9.3 -16 52.1 -6.7 0 -3.6 ATAGCAATTCCCTGCATGCA

8400 SEQ ID N0:3092 -9.3 -25.9 72.1 -14.7 -1.9 -10.8 AGCAATAGCAATTCCCTGCA

8404 SEQ ID N0:3093 -9.3 -25.2 70.3 -14.2 -1.7 -7.5 TGAAAGAAGATGAGTTTAGA

8794 SEQ ID N0:3094 -9.3 -16 52.7. -6.7 0 -2.2 GACCTCACATAAATCCAAGG

8961 SEQ ID N0:3095 -9.3 -21.6 62.1 -12.3 0 -3.7 CCTTATATAATTTATTATGG

9072 SEQ ID N0:3096 -9.3 -16.3 52.6 -6.2 -0.6 -5.3 TAAATGGAAGGTTCTTTCCA

642 SEQ ID N0:3097 -9.2 -20.5 61.4 -8.4 -2.9 -9.1 AAAAGTTTTCTTATTGATAT

722 SEQ ID N0:3098 -9.2 -14.9 50.1 -4.6 , -1 -5.7 ATAAAAGTTTTCTTATTGAT

724 SEQ ID N0:3099 -9.2 -14.9 50.1 -4.6 -1 -6.3 ATATACAAGGCAGAGGTGGC

781 SEQ ID N0:3100 -9.2 -23.3 68.6 -14.1 0 -4 TTGGTCAAAATAGTGCACAT

874 SEQ ID N0:3101 -9.2 -19.8 59.8 -9.6 0 -9.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GATTTTTATAAGTGACTCAA

971 SEQ ID N0:3102 -9.2 -l7 54.7 -7.8 0 -4.4 CCAAGATTTTTATAAGTGAC

975 SEQ ID N0:3103 -9.2 -17.7 55.6 -8.5 0 -4.1 ATTTATTCCTCAGATTGCCC

1281 SEQ ID N0:3104 -9.2 -24.9 71.5 -15.7 0 -3 TCCCATTTGAATCCATTGTG

1362 SEQ TD N0:3105 -9.2 -23.6 67.2 -14.4 0 -2.3 CAGATGTATCCTTCTGGACA

1507 5EQ ID N0:3106 -9.2 -23.6 69.6 -13 -1.3 -6.6 TTTTTGTTCTGCTTCTTCCA

1775 SEQ ID N0:3107 -9.2 -24.3 73.2 -15.1 0 -3.6 TGCTTTCGCCGTCTTCAAAT

2271 SEQ ID N0:3108 -9.2 -25 69.4 -15.1 -0.5 -3.6 CTCTCCTGCTTTCGCCGTCT

2277 SEQ ID N0:3109 -9.2 -30.7 83.6 -20.8 -0.5 -3.6 GAGTCTCTCCTGCTTTCGCC

2281 5EQ ID N0:3110 -9.2 -29.6 84 -19.7 -0.5 -4.1 CATCTTCCCATTTGCTGGAA

2384 SEQ TD N0:3111 -9.2 -25.2 71 -14.9 -1 -4.4 ATCCACAGTGCTGTGCATCT

2399 SEQ 2D N0:3112 -9.2 -26.9 77.8 -15.5 -l.7 -12.4 CCATTGCAATCCACAGTGCT

2407 SEQ ID N0:3113 -9.2 -26.5 73.4 -16.3 -0.8 -9 ATTGGCAAATCTATACCAGC

2657 SEQ ID N0:3114 -9.2 -21.8 63.8 -11.9 -0.4 -4.4 CATGGCCATAAAGAGGGTAT

2789 SEQ ID N0:3115 -9.2 -22.6 65.1 -12.2 0 -10.4 AAATCCCAGTAAAGACCAGG

2859 SEQ ID N0:3116 -9.2 -21.9 62.2 -12.7 0 -3.6 GACCTCCATACAGTCCCACA

3314 SEQ ID N0:3117 -9.2 -28.6 77.9 -19.4 0 -3.3 CAATAAGGCACATGGTTTGG

3339 SEQ ID N0:3118 -9.2 -21.4 62.8 -12.2 0 -5.2 TCCAGTATTATTGGACATGC

3614 SEQ ID N0:3119 -9.2 -22.4 66.7 -12 -1.1 -7.9 GCAGGTTTTTCGAAGATTCC

4049 SEQ ID N0:3120 -9.2 -23.5 68.6 -13.6 -0.4 -6.8 ACAATGAAAGTCTCAAACCA

4090 SEQ ID N0:3121 -9.2 -18.5 55.8 -8.7 -0.3 -3.1 TTTGTCAGCATATTCTAGCA

4187 SEQ ID N0:3122 -9.2 -22.3 68.2 -12.1 -0.9 -4.1 GCTCCAACAAGAGCATTCAC

4444 SEQ ID N0:3123 -9.2 -23.8 68.4 -12.1 -2.5 -7.8 TTCTGTTCCTCTGTCATAAA

4921 SEQ ID N0:3124 -9.2 -21.9 66.4 -12.7 0 -1.8 AAAGGTCTCAAAGTTGAACA

5537 SEQ ID N0:3125 -9.2 -18.1 56.1 -8 -0.8 -6.5 AGCAGAGGTTGTAATTTGGA

5579 SEQ ID N0:3126 -9.2 -21.8 66.1 -12.6 0 -5.7 AGTTTAGAGAACTCTATAAA

5g90 SEQ ID N0:3127 -9.2 -16 52.6 -5.6 -1.1 -8 GAGTGGAGTTCCCATTTAGT

6315 SEQ ID N0:3128 -9.2 -25.1 74.4 -14.6 -1.2 -6.4 GGAGTGGAGTTCCCATTTAG

6316 SEQ ID N0:3129 -9.2 -25.1 73.5 -14.6 -l.2 -6 GTCTGGTTTTGTTACACTAT

6383 SEQ ID N0:3130 -9.2 -21.9 67.8 -12.7 0 -2.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAACTTTTCCTTGTCTGGTT

6395 SEQ ID N0:3131 -9.2 -22.4 67 -13.2 0 -3.5 CTCTTTTCCTTTGCTTTCTT

6434 5EQ ID N0:3132 -9.2 -24.3 73.2 -15.1 0 -3.6 GCTTCACAGAGTTGCAGTGA

6633 SEQ TD N0:3133 -9.2 -25 74.7 -14.5 -1.2 -7.2 CTGTTTCTTCAACGTAAACC

6916 SEQ ID N0:3134 -9.2 -20.8 61.4 -10.5 -1 -6.6 TAAAAATAATCCTTTAAATA

7139 SEQ ID N0:3135 -9.2 -11.4 42.2 -2.2 0 -4.3 GATTACTTTTTGGAAAGCAT

7231 SEQ ID N0:3136 -9.2 -18.8 57.9 -8.7 -0.7 -4.6 GCTGAGAGCAGAAGATGGCA

7329 SEQ ID N0:3137 -9.2 -24.6 71.1 -14 -1.3 -6.5 CAGCATAGGCAATATTAACA

7561 SEQ ID N0:3138 -9.2 -19.6 59 -8.8 -l.6 -6.7 GAATTCATTCTATCACCAAT

7633 SEQ ID N0:3139 -9.2 -19.5 59.2 -10.3 0 -6.7 GCAGGTTGAATTCATTCTAT

7640 SEQ ID N0:3140 -9.2 -21.2 64.6 -11.3 -0.4 -8.4 AAATTATTCTGCTTGACATA

7669 SEQ ID N0:3141 -9.2 -17.8 55.9 -8.6 0 -3.6 GCAAAATATGAAGAATAGGT

7797 SEQ ID N0:3142 -9.2 -16 51.1 -6.8 0 -3.4 AAACAAAAATTTATATCATG

8312 SEQ ID N0:3143 -9.2 -11.4 42.2 -2.2 0 -5.2 GTTTAGATGCTTATAGCCAA

8781 5EQ ID NO:3144 -9.2 -21.7 64.9 -11.2 -1.2 -4.6 TATACATAGGAGTTTATATA

8884 SEQ ID N0:3145 -9.2 -16.3 53.8 -7.1 0 -3.2 GCTTGGCTTCAGTTTTCTTG

236 SEQ ID N0:3146 -9.1 -24.8 74.9 -15.7 0 -4.4 CTCATCCCTGTCAAACCTTG

261 SEQ ID N0:3147 -9.1 -25.4 70.9 -16.3 0 -2.5 CTCCAGGTCCCTTCTGTGAA

369 SEQ ID N0:3148 -9.1 -28.2 79.2 -18.6 -0.2 -4.2 GCTTTGGTTTGTTCTCATCA

603 SEQ TD N0:3149 -9.1 -24.3 74 -15.2 0 -2.8 TGTCTCCATAAATAAATGGA

654 SEQ ID N0:3150 -9.1 -18 55.3 -6.8 -2.1 -7.5 TTCATTACTATAAAAGTTTT

733 SEQ ID N0:3151 -9.1 -14.9 50.1 -5.2 -0.3 -5.9 AAATATACAAGGCAGAGGTG

783 SEQ ID N0:3152 -9.1 -18.9 57.7 -9.8 0 -4 ACATTGCCCAGGTCCACAAA

1087 SEQ ID N0:3153 -9.1 -26.5 71.8 -17.4 0 -3.6 GCCACTGCAAACATTTATTC

1293 SEQ ID N0:3154 -9.1 -22 64 -12.1 -0.6 -4.7 TTGGGGGCCACTGCAAACAT

1299 SEQ ID N0:3155 -9.1 -26.5 72 -15.9 -1.4 -6.9 CTTCCAGTTAAATGTGCTCA

1400 SEQ ID N0:3156 -9.1 -22.7 67 -13.6 0 -5.6 AATGTCAACTGGTAAAGATT

1630 SEQ ID N0:3157 -9.1 -17.4 54.7 -8.3 0 -3.2 CATGTCTGTGCGGCACAAAC

2304 SEQ ID N0:3158 -9.1 -24.4 68.6 -12.7 -1.4 -13.2 GTTGTCCAGTAGGTGACGTT

2460 SEQ ID N0:3159 -9.1 -25.8 76 -15.6 -1 -5.4 kcal/

mol kcal/moldeg kcal/mol'kcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CTTAACCTTCTCTTTCTGAC

2512 SEQ ID N0:3160 -9.1 -22.1 66.7 -13 0 -2.6 CTCCAGCATCTCCATTGAAA

2543 SEQ ID N0:3161 -9.1 -24.2 68.6 -15.1 0 -3.3 CACTGGTGGTTCCATTCCCA

3669 SEQ ID N0:3162 -9.1 -29 80 -18.7 -1.1 -6.4 ATCATTTTCATCGATTACGT

3719 SEQ ID N0:3163 -9.1 -20.3 61.1 -10.3 -0.7 -6.3 GCCTTCTTCTGTACTTACTT

4013 SEQ ID N0:3164 -9.1 -24.5 73.4 -15.4 0 -4.8 CTCAAACCAGTTGTGCTCAA

4079 SEQ ID N0:3165 -9.l -23.1 66.8 -13.3 -0.5 -6.4 TAGCCAGCACCAGGCATTAG

4277 SEQ ID N0:3166 -9.1 -27.1 75.8 -15.3 -2.7 -7.4 CCAACAGCACATTCATGATA

4473 SEQ ID N0:3167 -9.1 -21.8 63.3 -12.7 0 -6.4 GACCAACAGCACATTCATGA

4475 SEQ ID N0:3168 -9.1 -22.9 65.7 -13.8 0 -6.1 ACAGACCAACAGCACATTCA

4478 SEQ ID N0:3169 -9.l -23.2 66.4 -14.1 0 -4.1 TTTCTTGGATCCAAGTTTCT

4961 SEQ ID N0:3170 -9.1 -22.5 67.9 -9.7 -1.3 -15.5 GCTGGGCGAGGTATGGGTTT

4987 SEQ ID N0:3171 -9.1 -28.2 79.4 -19.1 0 -3.7 CAATTCCAGCTTCCTTTTTA

5511 SEQ ID N0:3172 -9.1 -23.4 68.2 -14.3 0 -4.5 GTTCTCCAGGATGACCGCGA

5771 SEQ ID N0:3173 -9.1 -28.3 76.2 -17.8 -1.3 -7.2 CTCTGCACTTTCTTCAGTAG

5801 SEQ ID N0:3174 -9.1 -23.4 71.8 -13.8 -0.1 -4.8 ATCTCAAAGTCATCCTCACT

5827 SEQ ID N0:3175 -9.1 -22.8 68 -13.7 0 -2.6 CGGCAGACACCTCCTCTTGT

6165 SEQ ID N0:3176 -9.1 -29 79.4 -19.4 -0.1 -4 ATATTTTTTAACCTTTGCTT

6220 SEQ ID N0:3177 -9.1 -19.3 59.5 -10.2 0 -3.6 TTGCCTCTTTGTTATAGTTA

6246 SEQ ID N0:3178 -9.1 -22 67.6 -12.9 0 -3 GTTTTTTCTGGAGTGGAGTT

6325 SEQ ID N0:3179 -9.1 -23.1 71.6 -14 0 -2.7 TTCCTTTGCTTTCTTTTTCT

6429 SEQ ID N0:3180 -9.1 -23.5 71.5 -14.4 0 -3.6 TGAGTATTTGTTAAAACAGT

6574 SEQ ID N0:3181 -9.1 -16.7 54 -7.6 0 -6.1 ATGAATAAACTATGAATTGG

6868 SEQ ID N0:3182 -9.1 -14.2 47.6 -5.1 0 -3.1 GACATAGCATTATGAATAAA

6879 SEQ ID N0:3183 -9.1 -15.7 50.8 -5.1 -1.4 -5 TGTATACTGTTTCTTCAACG

6922 SEQ ID N0:3184 -9.1 -19.9 61 -9.7 -1 -7.3 TCATTTGAGAGACAGGGTTC

6943 SEQ ID N0:3185 -9.1 -22.1 67.8 -13 0 -3.1 TTTCAAAATTAAAGCCATCT

7056 SEQ ID N0:3186 -9.1 -17.4 53.9 -8.3 0 -3.2 CTTTCAAAATTAAAGCCATC

7057 SEQ ID N0:3187 -9.1 -17.4 53.9 -8.3 0 -3.9 ATTGACATAGACTATCAGCT

7351 SEQ ID N0:3188 -9.1 -20.5 62.9 -11.4 0 -4.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATTAACATGCATTAGTGATA

7548 SEQ ID N0:3189 -9.1 -17.8 56.2 -8.7 0 -6.8 GAGCAGGTTGAATTCATTCT

7642 SEQ ID N0:3190 -9.1 -22.1 66.9 -12.3 -0.4 -8.4 ATTTAAACAAGTTTTAGACA

7825 SEQ ID N0:3191 -9.l -15.4 50.8 -5.6 -0.4 -5.5 AGGAAATTATCCATTGAAAA

7995 SEQ ID N0:3192 -9.1 -15.9 50.6 -4.6 -2.2 -7.2 AGCTTTAGTGCAGCAGGAAT

8222 SEQ ID N0:3193 -9.1 -23.9 70.7 -13.2 -1.5 -6.6 GCTCAATAATGACGTAGCTC

8436 SEQ ID N0:3194 -9.1 -21.7 64.1 -11.9 -0.5 -6.7 TCTCTTCTGCAGCACGTTTT

549 SEQ ID N0:3195 -9 -25.5 74.9 -16 0 -8.3 TTCTCTTCTGCAGCACGTTT

550 SEQ ID N0:3196 -9 -25.5 74.9 -16 0 -8.3 TTTCTCTTCTGCAGCACGTT

551 SEQ ID N0:3197 -9 -25.5 74.9 -16 0 -8.3 TTCTCATCATCATTATCTTG

592 SEQ ID N0:3198 -9 -19.9 62.8 -10.9 0 -1.6 CCATAAATAAATGGAAGGTT

649 SEQ ID N0:3199 -9 -16.9 52.4 -6.8 -1 -5.3 ATTACTATAAAAGTTTTCTT

730 SEQ ID N0:3200 -9 -15.1 50.7 -5.2 -0.8 -6.3 ATTCATTACTATAAAAGTTT

734 SEQ ID N0:3201 -9 -14.8 49.8 -5.2 -0.3 -4.3 CTTGGGGGCCACTGCAAACA

1300 SEQ ID N0:3202 -9 -27.4 73.8 -16.9 -1.4 -7.6 CATGGCCACCACAGCCAGGA

1727 SEQ ID N0:3203 -9 -30.5 80.3 -18.7 -2.8 -9.1 AGCTTCTTCCTGTTGCTTTT

1829 SEQ ID N0:3204 -9 -25.8 76.9 -16.3 -0.2 -4.3 TCCACTGAAATCTCTTGAAG

1874 SEQ ID N0:3205 -9 -20 60.2 -ll 0 -2.7 CTAACCCACCTACTCCACTG

1887 SEQ ID N0:3206 -9 -26.9 72.5 -17.9 0 -1.4 TTCTTTTGACGCTGTCTTCA

2055 SEQ ID N0:3207 -9 -23.7 70.6 -13.8 -0.8 -7.6 GAAGCTGCTTCTTTTGACGC

2063 SEQ ID N0:3208 -9 -24.4 70.3 -13.2 -1.2 -12.4 GGAAAACAGGGAGCCACGGA

2156 SEQ ID N0:3209 -9 -24.9 67.4 -15.3 -0.3 -4.3 TCCAGCATCTCCATTGAAAT

2542 SEQ ID N0:3210 -9 -23.3 66.7 -14.3 0 -4.1 GCAAAI~ATGAAGACGATGAT

3142 SEQ ID N0:3211 -9 -16.4 50.9 -7.4 0 -3.5 AGTCGTTCATGTGCCACCGT

3237 SEQ ID N0:3212 -9 -28.7 78.9 -19.7 0 -4.7 CACGCGGAACACAATCAGGA

3269 SEQ ID N0:3213 -9 -23.4 64.2 -13.9 0 -7.9 TTATTTTTCACATAATCAAT

3514 SEQ ID N0:3214 -9 -15.6 51.4 -6.6 0 -2.1 TAAAGTCTTCTTCGGGTTCA

3942 SEQ ID N0:3215 -9 -22.3 67 -12.6 -0.5 -3.8 TGATAGTCTTTCGCTGTTCA

4161 SEQ ID N0:3216 -9 -23.3 70.3 -14.3 0 -3.4 AAACATCAACGATCAAGAAA

4299 SEQ ID N0:3217 -9 -15.1 48.6 -6.1 0 -4.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAAGAAACATCAACGATCAA

4303 5EQ ID N0:3218 -9 -15.1 48.6 -6.1 0 -4.9 GGCTCTTAGAGGTCTTAAAG

4394 SEQ TD N0:3219 -9 -21.7 66.3 -12.1 -0.3 -5.3 GCACATTCATGATAGAGGGA

4467 SEQ ID N0:3220 -9 -22.5 66.8 -13.5 0 -6.4 TACTTTCACGTTTTTCCACC

4646 SEQ TD N0:3221 -9 -24 69.2 -15 0 -4.7 CCTCCAAACTTCTTTTTCTG

4891 SEQ ID N0:3222 -9 -22.8 66.5 -13.8 0 -1.8 CAGATGAGGATCATGATGCT

5062 SEQ ID N0:3223 -9 -22.1 65.6 -11 -2.1 -8.5 TTTCCACCATCATGGTGACC

5091 SEQ ID N0:3224 -9 -26.5 74 -13.8 -3.7 -11.1 TCAGCACAAATTCTCCAGTG

5178 5EQ ID N0:3225 -9 -22.8 66.9 -13.3 -0.2 -4.5 CCTTTGATCAGACGTAGGAT

5362 SEQ ID N0:3226 -9 -23.1 67.1 -14.1 0 -6.5 GAGCAGGAGGCCGATGTTAA

5438 SEQ ID N0:3227 -9 -25.6 71.6 -15.7 -0.8 -7.7 GGACATCCCAAAGATGGCAT

5477 SEQ ID N0:3228 -9 -24.7 68.3 -13.4 -2.3 -8 AGATCATGCTGTTGCCAAAG

5553 5EQ ID N0:3229 -9 -22.8 66.2 -12.9 -0.8 -6.6 TTAATTGCCTCTTTGTTATA

6250 SEQ ID N0:3230 -9 -20.1 61.8 -11.1 0 -3 TCCTTTGCTTTCTTTTTCTG

6428 5EQ ID N0:3231 -9 -23.4 70.9 -14.4 0 -3.6 TGGGTTTCTCCTCAGCAGTG

6699 SEQ ID N0:3232 -9 -27.2 80.3 -17.3 -0.8 -5.7 TTGGGGACCATGGAAATGCA

6852 SEQ ID N0:3233 -9 -24.1 67 -12.7 -2.4 -9.9 GAATTGGGGACCATGGAAAT

6855 SEQ ID N0:3234 -9 -21.5 61.4 -10.1 -2.4 -8.8 CAATACTTTAGATATATTTT

7269 SEQ ID N0:3235 -9 -15 50.4 -6 0 -4.3 TGCTGAGAGCAGAAGATGGC

7330 SEQ ID N0:3236 -9 -23.9 69.8 -13 -1.9 -6.5 GTCCATGTTATATTTGTAAT

8242 SEQ ID N0:3237 -9 -19.4 60.4 -10.4 0 -4.3 TTTTTAGCAATAGCAATTCC

8409 SEQ ID N0:3238 -9 -19.9 60.5 -9.2 , -1.7 -4.6 AGCTATTGTAGGTGGTTACT

8741 SEQ ID N0:3239 -9 -23.1 70.8 -13.6 -O.l -4.4 TGACAGGTATCTCATCCCTG

271 SEQ ID N0:3240 -8.9 -25.3 73.2 -14.6 -1.8 -6.1 CACATTTAATTACGTGTAGC

440 SEQ ID N0:3241 -8.9 -19.5 59.4 -10.6 0 -5 AGTAAAAAGGCGGAAGCTTT

506 5EQ ID N0:3242 -8.9 -19.9 58.6 -8.6 -2.4 -7.2 TCATCATCATTATCTTGTTC

589 SEQ ID N0:3243 -8.9 -20.2 64.1 -11.3 0 -1.9 AGCTTCCAAGTCACTATTTG

623 SEQ ID N0:3244 -8.9 -22.5 67.4 -13.6 0 -4.3 AATATACAAGGCAGAGGTGG

7g2 SEQ ID N0:3245 -8.9 -20.8 62.2 -11.9 0 -4 AAATACACAGTTGGTCAAAA

884 SEQ ID N0:3246 -8.9 -16.8 52.9 -7.9 0 -3.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GGTTGCTCAAGGTCATAAAT

900 SEQ ID N0:3247 -8.9 -21.4 64 -12.5 0 -3.6 AGGAGGGTTGCTCAAGGTCA

905 SEQ TD N0:3248 -8.9 -26.1 76.9 -15.2 -2 -4.7 GTCCAGTCAGGAGGGTTGCT

913 SEQ ID N0:3249 -8.9 -28.8 84.6 -18.4 -1.4 -5.9 GTGACTCAAAGGTATAGATT

960 SEQ ID N0:3250 -8.9 -19.1 59.5 -10.2 0 -3.1 AAATTGTTTTCAGTGCTCGG

1134 SEQ ID N0:3251 -8.9 -21.6 64.3 -12.7 0 -4.5 GAGCAAACACGCTCAGACAG

1239 SEQ ID N0:3252 -8.9 -22.9 65.3 -11.5 -2.5 -7.8 ACACAGATGTATCCTTCTGG

1510 SEQ ID N0:3253 -8.9 -23.2 68.8 -12.7 -1.6 -5.3 TTGTTCTGCTTCTTCCAAGG

1772 SEQ ID N0:3254 -8.9 -24.5 72.5 -15.1 -0.1 -3.6 GAAGCCCTGGCACCATCCTG

2367 SEQ ID N0:3255 -8.9 -30.5 80 -20.7 -0.7 -8.2 ACACATTGGCAAATCTATAC

2661 SEQ ID N0:3256 -8.9 -19.1 58.2 -10.2 0 -4.4 AACCATGCATCACAGCAGTC

2692 SEQ ID N0:3257 -8.9 -24.8 71.3 -15 -0.7 -7.5 CACAAGATGTTTTACTTTTA

2711 SEQ ID N0:3258 -8.9 -17.8 56.5 -8.9 0 -3 AAACTCTAAGCAGTCTGAAT

3027 SEQ ID N0:3259 -8.9 -18.9 58.2 -10 0 -4.1 TCCTAGAGCCCCCACAGAAT

3101 SEQ ID N0:3260 -8.9 -28.9 76.7 -20 0 -3.8 AAGGTGAGGTTTCCTAGAGC

3112 SEQ ID N0:3261 -8.9 -24.3 72.5 -14.7 -0.5 -4.4 ACTGGTGGTTCCATTCCCAT

3668 SEQ ID N0:3262 -8.9 -28.3 79 -18.2 -1.1 -6.6 CTGGGGTTGTTTATGAATGA

3745 SEQ ID N0:3263 -8.9 -21.4 64.2 -12.5 0 -1.8 CTTCAAAGGCCAATGCACCA

4131 SEQ ID N0:3264 -8.9 -25.1 68.5 -14.7 -1.4 -7.7 CAACGATCAAGAAATCTAGC

4293 SEQ ID N0:3265 -8.9 -18 54.9 -9.1 0 -4.9 ATGTCCGTAATGATTTGATG

4368 SEQ ID N0:3266 -8.9 -20 59.9 -11.1 0 -2.3 CAGACCAACAGCACATTCAT

4477 SEQ ID N0:3267 -8.9 -23 65.9 -14.1 0 -4.1 ACTTGCCAGCAAACAAATTC

4533 SEQ ID N0:3268 -8.9 -20.8 60.5 -11.9 0 -7.4 GTACAGATTTTCTTCATATA

4781 SEQ ID NO:3269 -8.9 -18.6 59.6 -9.7 0 -4.6 TGTTTGCTGGGCGAGGTATG

4992 SEQ ID N0:3270 -8.9 -25.8 73.8 -16.9 0.1 -4 GATGAGGATCATGATGCTGA

5060 SEQ ID N0:3271 -8.9 -22 65.4 -11 -2.1 -8.5 CATGGTGACCATGTTGAGGC

5081 SEQ ID N0:3272 -8.9 -25.5 73 -14 -2.6 -10.3 GCTCTGCACTTTCTTCAGTA

5802 SEQ ID N0:3273 -8.9 -25.2 76.3 -15.8 -0.1 -4.8 AGAACTCTATAAACTGGGTC

5883 SEQ ID N0:3274 -8.9 -19.4 59.8 -10.5 0 -2.6 ATCAGAGAGTTTAGAGAACT

5897 SEQ ID N0:3275 -8.9 -19 60.2 -8.6 -1.4 -5.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Tntra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGGGCAGATCCATGGCAATA

5973 SEQ ID N0:3276 -8.9 -25.3 71 -15.4 -0.9 -8 AATGATAGCGGCAGACACCT

6173 SEQ ID N0:3277 -8.9 -24.5 68.2 -15.6 0 -6.6 ATAGGTAAGTCAATCCTCCC

6271 SEQ ID N0:3278 -8.9 -24.4 70.5 -15 -0.2 -3.5 TAGAATGGATGCAAGGACTG

6787 SEQ ID N0:3279 -8.9 -20.1 60.1 -11.2 0 -5.6 CCATTACAATTCTTTTTCTG

7009 SEQ TD N0:3280 -8.9 -19.8 60.5 -10.9 0 -2.5 CTGTTCAGATAGAAACAAAC

7093 SEQ ID N0:3281 -8.9 -16.5 52.7 -6.8 -0.6 -4.1 ACTGTTCAGATAGAAACAAA

7094 SEQ ID N0:3282 -8.9 -16.5 52.7 -6.8 -0.6 -5.6 AACTGTTCAGATAGAAACAA

7095 SEQ TD N0:3283 -8.9 -16.5 52.7 -6.8 -0.6 -5.6 TTAGAGGAGACTTTACAGGC

7119 SEQ ID N0:3284 -8.9 -21.7 66.2 -11.9 -0.7 -5.4 CTTTAAATATTAGAGGAGAC

7128 SEQ ID N0:3285 -8.9 -16.1 52.6 -7.2 0 -4.3 TTTGACCTAAATTAAATATA

7212 SEQ ID N0:3286 -8.9 -14 47.3 -5.1 0 -3.2 GTTCATGGCTAAAAAGAACA

8264 SEQ ID N0:3287 -8.9 -18 55.2 -7.7 -1.3 -5.7 ATTCTTTTGGCTCAATAATG

8445 SEQ ID N0:3288 -8.9 -19 58.8 -10.1 0 -5.3 AATTATATTGAAAGAAGATG

8802 SEQ ID N0:3289 -8.9 -12.6 44.7 -3.7 0 -2.3 CAATTATATTGAAAGAAGAT

8803 SEQ ID N0:3290 -8.9 -13.3 46 -3.7 -0.4 -3.4 TTTCTCTGTGGATAAGAAAA

48 SEQ TD N0:3291 -8.8 -17.3 54.8 -7.4 -1 -4.5 GCTCCAGGTCCCTTCTGTGA

370 SEQ ID N0:3292 -8.8 -30.7 86.5 -21.4 -0.2 -4 GATTCTCTAGTAAAAAGGCG

514 SEQ ID N0:3293 -8.8 -18.9 57.5 -10.1 0 -4 TTGGGCTTCTTGGCTTTCTC

565 SEQ ID N0:3294 -8.8 -26.5 78.9 -16.7 -0.9 -3.7 GCTTCCAAGTCACTATTTGG

622 SEQ ID N0:3295 -8.8 -23.7 69.8 -13.6 -1.2 -5.2 TATACAAGGCAGAGGTGGCA

780 SEQ ID N0:3296 -8.8 -24 69.8 -14.3 -0.8 -4.1 AAATCTTGATAGCAATTTTC

825 SEQ ID N0:3297 -8.8 -16.8 54 -7.5 -0.1 -0.3 CTCAGATTGCCCATGAACAG

1273 SEQ ID N0:3298 -8.8 -24 67.9 -15.2 0 -4.5 CCAAGGTGGCCTGATTCTGC

1758 SEQ ID N0:3299 -8.8 -28.1 77.8 -18.6 -0.4 -7.2 TTTTTTGTCACTGGTCAGTC

2105 SEQ ID N0:3300 -8.8 -23 71.8 -14.2 0 -6.6 ACTGATGAGGGGAGCAGAAT

2124 SEQ ID N0:3301 -8.8 -22.9 66.9 -13.4 -0.5 -4.1 AAATGCTTGTTTTGCTATTG

2184 SEQ ID N0:3302 -8.8 -19.4 59.6 -9.9 -0.4 -4.3 GCTATGCTCACGGCTCTTTG

2578 SEQ ID N0:3303 -8.8 -26.6 75.7 -17.1 -0.4 -4.7 TAACCATGCATCACAGCAGT

2693 SEQ ID N0:3304 -8.8 -24.1 69.1 -14 -1.2 -6.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ACATTTGACAGACCAAGCTC

2983 SEQ ID N0:3305 -8.8 -22.5 65.8 -13.7 0 -5 CACATTTGACAGACCAAGCT

2984 SEQ ID N0:3306 -8.8 -22.8 65.5 -14 0 -4.8 AATGATCGCAGTACAGACAA

3010 SEQ ID N0:3307 -8.8 -20.2 59.9 -10.7 -0.4 -5.7 TGAGGTTTCCTAGAGCCCCC

3108 SEQ ID N0:3308 -8.8 -30.6 83.1 -21.1 -0.5 -4.4 TAATCAATTCCCTTTTGCAT

3502 SEQ ID N0:3309 -8.8 -21.6 63.1 -12.8 0 -5.1 ATAATTAAGCTCTTTGCTTA

3641 SEQ ID N0:3310 -8.8 -18.8 58.8 -7.5 -2.5 -8 CATTTTCATCGATTACGTAT

3717 SEQ ID N0:3311 -8.8 -19.6 59.2 -9.9 -0.7 -6.3 ACAGCAATTGGCACTGTGAC

3772 SEQ ID N0:3312 -8.8 -23.6 68.4 -13.2 -1.5 -8.7 TCTTAGAGGTCTTAAAGCTC

4391 SEQ TD N0:3313 -8.8 -20.9 65.2 -12.1 0.7 -5 AACAGCACATTCATGATAGA

4471 SEQ ID N0:3314 -8.8 -19.7 59.9 -10.9 0 -6.4 AATGTCAAACATGTTACCCG

4577 SEQ ID N0:3315 -8.8 -21.1 60.4 -11.1 -1.1 -9 CAGTGCAAGATAGCCAGCGC

4682 SEQ TD N0:3316 -8.8 -26.9 74.4 -17.2 -0.7 -7.3 TCCTCTGTCATAAAGATGTC

4915 SEQ ID N0:3317 -8.8 -21.4 65.2 -11.9 -0.4 -3.1 CTGGTCATCCGTTTCCACCA

5102 SEQ TD N0:3318 -8.8 -29 79 -19 -1.1 -5.4 TGCCCTGGTCATCCGTTTCC

5106 SEQ ID N0:3319 -8.8 -31.2 83.9 -21.2 -1.1 -4.4 AGTAGTAGTGTCTGAGGGAA

5205 SEQ ID N0:3320 -8.8 -22.4 69.6 -13.6 0 -2.9 CAACAGATGGGTCCCCACGG

5691 SEQ ID N0:3321 -8.8 -28.4 74.6 -18.7 -0.6 -8.8 CCACAACCAGGAAGGATATG

5739 SEQ ID N0:3322 -8.8 -22.4 63.3 -12.8 -0.6 -4.4 GAGTTTAGAGAACTCTATAA

5891 SEQ ID N0:3323 -8.8 -17.3 55.9 -5.6 -2.9 -8.5 GCCATAAACCTGTCTTCCAT

6091 SEQ ID N0:3324 -8.8 -25.6 71.1 -16.8 0 -2 ATAGGAAGGAGGAGGGGTGG

6362 SEQ ID N0:3325 -8.8 -24.4 71.1 -15.6 0 -0.9 TCTGGTTTGTCTTTCTCAAA

6412 SEQ ID N0:3326 -8.8 -21.6 66.6 -12.3 -0.2 -6.4 TACTAAAGGTGTTTGGTGTA

6767 SEQ ID N0:3327 -8.8 -20.2 62.5 -10.5 -0.8 -6.2 ACTGTACTAAAGGTGTTTGG

6771 SEQ ID N0:3328 -8.8 -20.4 62.5 -11.6 0 -5.1 TATACTGTTTCTTCAACGTA

6920 SEQ ID N0:3329 -8.8 -19.6 60,5 -9.7 -1 -5.3 TAACTGTTCAGATAGAAACA

7096 SEQ ID N0:3330 -8.8 -16.9 53.9 -7.4 -0.5 -5.6 TTGACCTAAATTAAATATAG

7211 SEQ ID N0:3331 -8.8 -13.9 47.1 -5.1 0 -3.2 GCATTTGACCTAAATTAAAT

7215 SEQ ID N0:3332 -8.8 -17.1 53.1 -7,7 -0.3 -4.8 CTAGAATGGATTTATTAGAT

7248 SEQ ID N0:3333 -8.8 -16.6 53.7 -7.8 0 -3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ACCAATATGTGACATTCTTT

7619 SEQ ID N0:3334 -8.8 -20.2 61 -11.4 0 -3.9 ATGTTGAATTAAAAGTGCAA

7715 SEQ ID N0:3335 -8.8 -15.9 51.1 -7.1 0 -5.4 TAGCTTTAGTGCAGCAGGAA

8223 SEQ ID N0:3336 -8.8 -23.6 70.1 -13.,2 -l.5 -6.6 TACAGTAAATAGTTTTGTAA

8366 SEQ ID N0:3337 -8.8 -15.5 51.6 -6.7 0 -4.1 TTCTTCAATACACTACAGTA

8379 SEQ ID N0:3338 -8.8 -19.1 59.9 -10.3 0 -3.4 AAACCCCAGAGCCCAATAAG

8490 SEQ ID N0:3339 -8.8 -24.9 66.1 -16.1 0 -3.2 TTATTAGCCACCAAGTAATT

8835 SEQ ID N0:3340 -8.8 -20.6 61.1 -11.8 0 -3.2 TGGGAGAATTATGAAATACA

9055 SEQ ID NO:3341 -8.8 -16.5 52.3 -7.7 0 -3.2 TCTGTGAATTATCTCATTTA

357 SEQ ID N0:3342 -8.7 -18.5 58.9 -9.8 0.4 -3.2 TTCCGGAAAAGCTCCAGGTC

380 SEQ ID NO:3343 -8.7 -25.6 70.9 -15 -1.9 -9.8 ATAAATAAATGGAAGGTTCT

647 SEQ ID NO:3344 -8.7 -15.5 50.5 -6.8 0 -4.2 ATCCAGGTCCTCCAGGGGCT

695 SEQ ID N0:3345 -8.7 -32.2 88.8 -22.1 -1.3 -8.4 TTATTCATTACTATAAAAGT

736 SEQ ID N0:3346 -8.7 -14.4 49 -5.2 -0.1 -2.7 TTGTCCAGTCAGGAGGGTTG

915 SEQ ID N0:3347 -8.7 -26.2 77.8 -16.1 -1.3 -5.3 TCTTCTAAGCAAAACCCTCT

997 SEQ ID N0:3348 -8.7 -22.5 64.7 -13.8 0 -4.1 GAGAGCAAACACGCTCAGAC

1241 SEQ ID N0:3349 -8.7 -22.8 65.4 -10.8 -3.3 -8.5 AGATGTATCCTTCTGGACAC

1506 SEQ ID N0:3350 -8.7 -23.1 69 -13 -1.3 -5.8 CTTCACACAGATGTATCCTT

1514 SEQ ID N0:3351 -8.7 -22.8 67.8 -13.4 -0.5 -3.7 CTTGAGTCATGAGTCGAAAT

1599 SEQ ID N0:3352 -8.7 -20.1 60.7 -9.7 0 -11.6 TAATGTCAACTGGTAAAGAT

1631 SEQ ID N0:3353 -8.7 -17 53.9 -8.3 0 -3.2 CTCCTTTGTTGTTTCCTTCA

2007 SEQ ID NO:3354 -8.7 -25.6 75.7 -16.9 0 -1.7 TCTCTCTCCTTTGTTGTTTC

2012 SEQ ID N0:3355 -8.7 -24.5 76.1 -15.8 0 0 CAGTCTGTTTCCATCCATGG

2090 SEQ ID N0:3356 -8.7 -26 75.3 -16.6 -0.5 -7.7 GTCAGTCTGTTTCCATCCAT

2092 SEQ ID N0:3357 -8.7 -26.4 78.2 -17.7 0 -3.6 GGTGCCCTCTGGGGGAAGTT

2477 SEQ ID NO:3358 -8.7 -30.4 83.9 -19.5 -2.2 -8.7 AGCTTAACCTTCTCTTTCTG

2514 SEQ ID N0:3359 -8.7 -23.1 69.4 -14.4 0 -4.3 TTGGCAAATCTATACCAGCA

2656 SEQ ID N0:3360 -8.7 -22.5 64.9 -13.1 -0.5 -4.6 GGGTATTTAAGACAATGCAA

2775 SEQ ID NO:3361 -8.7 -19.1 58 -9.9 -0.1 -5.6 ACCAGGTTTCCTACAGTCAA

2845 SEQ ID NO:3362 -8.7 -25 72.4 -15.8 -0.1 -4.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGTAAAGACCAGGTTTCCTA

2852 SEQ ID N0:3363 -8.7 -22.6 66.6 -13.4 -0.1 -5.9 CCATCAAAGATATTCCAGCC

2938 SEQ ID N0:3364 -8.7 -23.7 66.7 -14.3 -0.4 -3.8 ACTTGAAAACTCTAAGCAGT

3033 SEQ ID N0:3365 -8.7 -18.8 57.8 -10.1 0 -4.1 CCACAGAATTGCCAATGATC

3090 SEQ ID N0:3366 -8.7 -22.4 63.7 -13.7 0 -3.9 GCTAAATGAACTCAATAATA

3416 SEQ ID N0:3367 -8.7 -15.1 49.4 -6.4 0 -3.9 ATAGTCTTTCGCTGTTCAAT

4159 SEQ TD N0:3368 -8.7 -22 66.6 -13.3 0 -3.1 CACCCTCATGCCTTCAAACC

4421 SEQ TD N0:3369 -8.7 -27.6 73.5 -18.9 0 -4.4 GGAATTGCTCCAACAAGAGC

4450 SEQ ID N0:3370 -8.7 -22.9 65.6 -12.1 -2.1 -7.7 TTTCACGTTTTTCCACCGAG

4643 SEQ ID N0:3371 -8.7 -24.6 68.9 -15.9 0 -4.7 TGATGCTGATATCAAAGACT

5049 SEQ ID N0:3372 -8.7 -l9 58.2 -9.6 -0.4 -7.7 GTTTCCACCATCATGGTGAC

5092 SEQ ID N0:3373 -8.7 -25.7 73.8 -13.8 -3.2 -10.6 AGAGTTTAGAGAACTCTATA

5892 SEQ ID N0:3374 -8.7 -18 58.1 -5.6 -3.7 -9.1 TCAGAGAGTTTAGAGAACTC

5896 SEQ ID N0:3375 -8.7 -19.4 61.7 -8.6 -2.1 -7.6 GAGGATCCAGGGCAGCTGCA

5919 SEQ ID N0:3376 -8.7 -29.6 82.5 -18 -1 -14 TAATAGGCTCATAAGAGACT

6126 SEQ ID N0:3377 -8.7 -19 58.9 -8.9 -1.3 -5.2 TAGCGGCAGACACCTCCTCT

6168 SEQ ID N0:3378 -8.7 -29.2 79.8 -20 -0.1 -6.6 TCAAACTTTTCCTTGTCTGG

6397 SEQ ID N0:3379 -8.7 -22.2 66.1 -13.5 0 -2.9 TTTACTTTTGATTTTCTCTG

6456 SEQ ID N0:3380 -8.7 -18.8 60.1 -10.1 0 -2.2 GTTTCTTTTTACTTTTGATT

6463 SEQ TD N0:3381 -8.7 -18.9 60.6 -10.2 0 -2.2 CTCTTGAAGTCCAGTTGACA

6536 SEQ ID N0:3382 -8.7 -23.3 69.2 -12.8 -1.8 -5.1 TCCTCTTGAAGTCCAGTTGA

6538 SEQ ID N0:3383 -8.7 -24.8 72.9 -16.1 0 -3 TAGGCACTGACTATGAGTAT

6587 SEQ ID N0:3384 -8.7 -21.4 65 -10.5 -2.2 -7.3 ATAGGCACTGACTATGAGTA

6588 SEQ ID N0:3385 -8.7 -21.4 65 -10.5 -2.2 -7.3 TATAGGCACTGACTATGAGT

6589 SEQ ID N0:3386 -8.7 -21.4 65 -10.5 -2.2 -7.3 TGTCTTGTATAGGCACTGAC

6596 SEQ TD N0:3387 -8.7 -22.8 69.3 -13.2 -0.8 -4.7 CATTGGGTTTCTCCTCAGCA

6702 SEQ ID N0:3388 -8.7 -26.8 77.9 -17.6 -0.1 -4.1 AACTATCCCTATAGTCTAGG

6726 SEQ ID N0:3389 -8.7 -22.2 66.3 -11.5 -2 -7.6 ACATAGCATTATGAATAAAC

6878 SEQ ID N0:3390 -8.7 -15.3 50.1 -5.1 -1.4 -5 TTCAAAATTAAAGCCATCTA

7055 SEQ ID N0:3391 -8.7 -17 53.1 -8.3 0 -3.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGTTCAGATAGAAACAAACA

7092 SEQ ID N0:3392 -8.7 -16.3 52.1 -6.9 -0.5 -4.4 TAACCAAGACTTGTGAAGAA

7600 SEQ ID N0:3393 -8.7 -18 55.1 -8.8 -0.2 -5.4 ATTTATATTTAAACAAGTTT

7831 SEQ ID N0:3394 -8.7 -13.7 47.4 -4.4 -0.3 -5.3 ATTTTCTTATGTACAAAATG

7883 SEQ ID N0:3395 -8.7 -15.1 50.2 -6.4 0 -6.8 TTTTATTTTAGGAAATTATC

8004 SEQ ID N0:3396 -8.7 -14.6 49.7 -5.1 -0.6 -4.4 TAACTTTTATTTTAGGAAAT

8008 SEQ ID N0:3397 -8.7 -14.5 49 -5.1 -0.4 -4.6 CATTATCTAACTTTTATTTT

8015 SEQ ID N0:3398 -8.7 -16.2 53.3 -7.5 0 -1.6 CTTTAGTGCAGCAGGAATTA

8220 SEQ ID N0:3399 -8.7 -21.9 65.8 -13.2 0 -6.6 TTCTTTTGGCTCAATAATGA

8444 SEQ ID N0:3400 -8.7 -19.6 60.1 -10.3 -0.3 -5 TAGTTTGTTGTAAAATTCAT

8601 SEQ ID N0:3401 -8.7 -16.4 53.6 -7 -0.4 -4.4 AAACCTGAAAGTTGGAACAT

8709 SEQ ID N0:3402 -8.7 -18.2 54.9 -8.7 -0.6 -4.8 ATAACGTGTCTTCCTCTGGC

142 SEQ ID N0:3403 -8.6 -25.4 73.3 -16.8 0 -5.3 TGGCTTCAGTTTTCTTGCTC

233 SEQ ID N0:3404 -8.6 -25.1 76.4 -15.8 -0.4 -3.7 GACAGGTATCTCATCCCTGT

270 SEQ ID N0:3405 -8.6 -26.5 77 -15 -2.9 -8.3 AAAGTTTTCTTATTGATATA

721 SEQ ID N0:3406 -8.6 -15.3 51.3 -5.6 -1 -4.6 TATTCATTACTATAAAAGTT

735 SEQ ID N0:3407 -8.6 -14.4 49 -5.2 -0.3 -2.8 CCCCCACAATGGTCTTTAAA

1167 SEQ ID N0:3408 -8.6 -25.1 67.9 -15.6 -0.7 -5.2 GCCTGATTCTGCTCCTCATA

1750 SEQ ID N0:3409 -8.6 -27.5 78.3 -18.9 0 -3.6 ATAATTAAGCTCTTTGCTTA

1832 SEQ ID N0:3410 -8.6 -27 79 -17.7 -0.5 -5.2 TCAGTCTGTTTCCATCCATG

2091 SEQ ID N0:3411 -8.6 -25.2 74.3 -16.6 0 -3.9 GAATTTTTTGTCACTGGTCA

2108 SEQ ID N0:3412 -8.6 -21.3 65.2 -12.7 0 -3.3 CAGTGCTGTGCATCTTCCCA

2394 SEQ ID N0:3413 -8.6 -28.8 81.3 -19.1 -1 -5.9 TTAACCTTCTCTTTCTGACT

2511 SEQ ID N0:3414 -8.6 -22.1 66.7 -13.5 0 -2.6 GAGCTTAACCTTCTCTTTCT

2515 SEQ ID N0:3415 -8.6 -23.7 70.9 -15.1 0 -5.2 AATTCACAAGATGTTTTACT

2715 SEQ TD N0:3416 -8.6 -17.6 55.8 -9 0 -4 TTCCATCAAAGATATTCCAG

2940 5EQ ID N0:3417 -8.6 -20.4 60.9 -11.1 -0.4 -3.9 TTGCCAATGATCTTAATTAG

3082 SEQ ID N0:3418 -8.6 -18.7 57.4 -10.1 0 -4.9 CTTATTTTTCACATAATCAA

3515 SEQ ID N0:3419 -8.6 -16.5 53.3 -7.9 0 -2.2 GTCTATCTTATTGCCTTCAT

3590 SEQ ID N0:3420 -8.6 -23.3 70.5 -14.7 0 -3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TAAGATAATTAAGCTCTTTG

3645 SEQ ID N0:3421 -8.6 -15.9 52.1 -6.8 -0.1 -6.1 ACTACTGAGAAGGATCATGA

4112 SEQ ID N0:3422 -8.6 -19.9 60.7 -10.4 -0.8 -7.8 TCTTTCGCTGTTCAATGTAT

4155 SEQ ID N0:3423 -8.6 -22 66.2 -13.4 0 -3.1 CTTAAAGCTCTTAATGTCCG

4381 SEQ ID N0:3424 -8.6 -20.8 61.3 -12.2 0 -5.9 CAAGAGCATTCACAACCACC

4437 SEQ ID N0:3425 -8.6 -23.6 66.2 -14.4 -0.3 -3.3 GGTAGAACTTGCCAGCAAAC

4539 SEQ ID N0:3426 -8.6 -23 65.9 -13.9 -O.l -7.5 TGATAAAGATGACAAAGTAT

4809 SEQ TD N0:3427 -8.6 -14.5 48.4 -5.9 0 -2.8 TCTTCATTGCATTGTAATAT

4944 SEQ ID N0:3428 -8.6 -19.1 59.7 -10.5 0 -6.2 ACCATCATGGTGACCATGTT

5086 SEQ ID N0:3429 -8.6 -25.2 71.6 -14 -2.6 -10.9 CACCATCATGGTGACCATGT

5087 SEQ ID N0:3430 -8.6 -25.8 72.3 -14 -3.2 -11.5 ATCCGTTTCCACCATCATGG

5096 SEQ ID N0:3431 -8.6 -26.9 73.8 -17.4 -0.8 -5.5 GTCTCAAAGTTGAACATGTC

5533 SEQ ID N0:3432 -8.6 -19.9 61.6 -11.3 0 -7 AAGCAGATCATGCTGTTGCC

5557 SEQ ID N0:3433 -8.6 -25.3 72.7 -13.9 -2.8 -10.6 GGGGCTCTGCACTTTCTTCA

5805 SEQ ID N0:3434 -8.6 -27.9 81.2 -19.3 ~ 4.6 -4.8 ACTTTTCCCAAACCTCATAG

5850 SEQ ID N0:3435 -8.6 -23 65.6 -14.4 0 -1.7 TTGGGTTTTGCTATGAGAAG

5941 5EQ ID N0:3436 -8.6 -20.9 63.6 -12.3 0 -3.6 AGGAACTCCCATCTGTTTTT

6339 SEQ ID N0:3437 -8.6 -24.2 70.3 -14.6 -0.9 -5.1 TTGTCTGGTTTTGTTACACT

6385 SEQ TD N0:3438 -8.6 -22.3 68.7 -13.7 0 -2.9 TTCTCAAACTTTTCCTTGTC

6400 SEQ ID N0:3439 -8.6 -21.5 65.5 -12.9 0 -2.8 TCTCCTCAGCAGTGTCAGCT

6693 SEQ ID N0:3440 -8.6 -28.4 84.6 -19 -0.6 -4.6 TTCTCCTCAGCAGTGTCAGC

6694 SEQ ID N0:3441 -8.6 -27.6 82.8 -l9 0 -4.2 ATTGGGTTTCTCCTCAGCAG

6701 SEQ ID N0:3442 -8.6 -26.1 77.1 -16.6 -0.8 -4.9 GCAAAACACCTTTGTCTGAT

6962 SEQ ID N0:3443 -8.6 -21.7 63.1 -11.7 -1.3 -5.9 ATTAAAGCCATCTAGAAAAT

7049 SEQ ID N0:3444 -8.6 -16.4 51.8 -7.8 0 -6.2 TATAGAGCTCTGAAACTTAG

7196 SEQ ID N0:3445 -8.6 -18.1 57.2 -8.5 0 -10 TTCTAGAATGGATTTATTAG

7250 SEQ ID N0:3446 -8.6 -16.5 54 -7.9 0 -7.2 GTTGAATTAAAAGTGCAAAA

7713 SEQ ID N0:3447 -8.6 -14.5 48 -5.9 0 -5,4 AACTTTTATTTTAGGAAATT

8007 SEQ ID N0:3448 -8.6 -14.9 49.8 -5.5 -0.6 -4.6 AATGAAATTTATTCTTTTGG

8455 SEQ ID N0:3449 -8.6 -15.3 50.6 -5.6 -1 -5.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Tnter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ACATAGGAGTTTATATAATG

8881 SEQ TD N0:3450 -8.6 -16.2 53 -7.6 0 -5.5 AAGAGACCTTTCCAGAATCC

26 SEQ ID N0:3451 -8.5 -23.2 66.6 -14.7 0 -2.9 GTCCCTTCTGTGAATTATCT

363 SEQ ID N0:3452 -8.5 -24.1 71.1 -15.1 -O.l -3.2 CATAAATAAATGGAAGGTTC

648 SEQ ID N0:3453 -8.5 -15.3 49.9 -6.8 0 -3.3 TTACTATAAAAGTTTTCTTA

729 SEQ TD N0:3454 -8.5 -14.8 50.1 -5.2 -1 -6.3 TGTCCAGTCAGGAGGGTTGC

914 SEQ ID N0:3455 -8.5 -27.9 82.2 -18 -1.3 -5.3 CACATATGCCATCACAATGA

1061 SEQ ID N0:3456 -8.5 -21.7 62.7 -12.5 -0.5 -8.3 TTTTCAGTGCTCGGAGAACT

1128 SEQ ID N0:3457 -8.5 -23.3 68.5 -14 -0.6 -6.4 CCATTTCCACAGAGTAAAGG

1471 SEQ ID N0:3458 -8.5 -22.2 64.4 -13.7 0 -3.3 ATGTATCCTTCTGGACACTG

1504 SEQ ID N0:3459 -8.5 -23.4 69.2 -14.9 1.5 -5.8 GATGTATCCTTCTGGACACT

1505 SEQ ID N0:3460 -8.5 -24 70.7 -15.5 l.5 -5.9 GGTAAAGATTTTCCCAATAG

1620 SEQ TD N0:3461 -8.5 -19.7 59.1 -11.2 0 -3.3 CATGTATGTTTTCCCAGCAG

1655 SEQ ID N0:3462 -8.5 -24.7 71.7 -16.2 0 -4.1 TCACCAAATAAAATGAGCCC

1701 SEQ ID N0:3463 -8.5 -20.6 58.6 -12.1 0 -3.2 TCCTCCATTCTTTAGCACCT

1953 SEQ ID N0:3464 -8.5 -27.3 77.4 -18.8 0 -4.1 AATCCACAGTGCTGTGCATC

2400 SEQ ID N0:3465 -8.5 -25.3 73.2 -14.6 -1.7 -12.4 GAAGTTGTCCAGTAGGTGAC

2463 SEQ ID N0:3466 -8.5 -23.6 71.2 -14.5 -0.3 -4.6 GCTTAACCTTCTCTTTCTGA

2513 SEQ ID N0:3467 -8.5 -23.7 70.5 -15.2 0 -2.8 GCTGGCTATGCTCACGGCTC

2582 SEQ ID N0:3468 -8.5 -29.4 82 -19.7 -1.1 -6.7 ACAATCCCTCCACATTTGAC

2994 SEQ TD N0:3469 -8.5 -24.5 68.6 -16 0 -3.2 CAGAATTGCCAATGATCTTA

3087 SEQ ID N0:3470 -8.5 -20.2 60.1 -11.7 0 -4.9 CCCACAGAATTGCCAATGAT

3091 SEQ ID N0:3471 -8.5 -24 65.8 -15.5 0 -4.3 TCTTATTTTTCACATAATCA

3516 SEQ TD N0:3472 -8.5 -17.6 56.5 -9.1 0 -2.2 CAGTAAAACAAGCTTCCGGT

3963 SEQ ID N0:3473 -8.5 -22.1 63 -13.6 0 -6.6 TGTACTTACTTGACAGAATG

4004 SEQ ID N0:3474 -8.5 -18.4 57.6 -9.9 0 -4.8 CTTCTTCTGTACTTACTTGA

4011 SEQ ID N0:3475 -8.5 -21.3 66.2 -12.8 0 -4.8 TATATAGGTAAAGACTTTGT

4202 SEQ ID N0:3476 -8.5 -16.9 54.7 -7.9 -0.1 -4.8 GCATTTCCAGAATGAATATA

4218 SEQ ID N0:3477 -8.5 -19 57.8 -8.9 -1.6 -6.4 AGAGCATTGGCTACCAGGCT

4327 SEQ ID N0:3478 -8.5 -27.6 78 -17.3 -1.8 -8.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGCATTCACAACCACCCTCA

4433 5EQ ID N0:3479 -8.5 -27 73.6 -18.5 0 -4.1 TAACATCTCGTGAATCAACA

4749 SEQ ID N0:3480 -8.5 -18.8 57 -10.3 0 -4.6 TTCCTCTGTCATAAAGATGT

4916 SEQ ID N0:3481 -8.5 -21.1 64.1 -11.9 -0.4 -3.1 AGTTTCTTCATTGCATTGTA

4948 SEQ ID N0:3482 -8.5 -21.5 66.9 -l3 0 -6.2 AAGGTCTCAAAGTTGAACAT

5536 SEQ ID N0:3483 -8.5 -18.8 58 -9.4 -0.8 -6.5 GGCAGCTGCAAAATCAGAGA

5909 SEQ ID N0:3484 -8.5 -23.1 66.5 -11.7 -0.7 -14 TTTTTTAACCTTTGCTTTAA

6217 SEQ ID N0:3485 -8.5 -18.7 57.9 -10.2 0 -3.6 GAGGAACTCCCATCTGTTTT

6340 SEQ ID N0:3486 -8.5 -24.7 71.3 -14.6 -1.6 -5.7 GGGGTGGTAGAGGAACTCCC

6349 SEQ ID N0:3487 -8.5 -28.1 79 -17.2 -2.4 -7.2 GTTTTGTTACACTATCATAG

6378 SEQ ID N0:3488 -8.5 -19 60.5 -10.5 0 -3.1 CTTTACCTTCATAGGCTGTA

6510 SEQ ID N0:3489 -8.5 -23.6 70.2 -14.6 -0.2 -3.7 ACTGACTATGAGTATTTGTT

6582 SEQ ID N0:3490 -8.5 -19.5 61.3 -10.5 -0.1 -3.8 TCAACGTTGATACCCTGCTT

6649 SEQ ID N0:3491 -8.5 -24.9 69.3 -14.5 0 -l2 AAAGCCATCTAGAAAATGGA

7046 SEQ ID N0:3492 -8.5 -18.4 55.5 -8.3 -1.6 -8.3 TGATACTCATGTTGAATTAA

7723 SEQ ID N0:3493 -8.5 -16.8 53.8 -8.3 0 -4.7 ATGATACTCATGTTGAATTA

7724 SEQ ID N0:3494 -8.5 -17.5 55.7 -8.3 -0.5 -4.7 AAAACAAAAATTTATATCAT

8313 SEQ ID N0:3495 -8.5 -10.7 40.8 -2.2 0 -5.2 GACGTAGCTCACCATTCTTT

8426 SEQ ID N0:3496 -8.5 -24.8 71.6 -16.3 0 -5.3 TATATTTTAAACTGCCAACA

8528 SEQ ID N0:3497 -8.5 -17.8 54.8 -9.3 0 -4.1 TATTAATTATATATATTTTA ?

8539 SEQ ID N0:3498 -8.5 -11.7 43.5 -3.2 0 -5.2 TCATTAATACCTTATGTATA

8900 SEQ ID N0:3499 -8.5 -17.4 55.4 -8 -0.8 -4.2 TCCCTGTCAAACCTTGATGT

257 SEQ ID N0:3500 -8.4 -25.2 70.7 -15.6 -1.1 -4.5 TACTATAAAAGTTTTCTTAT

728 SEQ ID N0:3501 -8.4 -14.7 49.8 -5.2 -1 -6.3 AAAAGTGACTGTCATCTCCA

1428 SEQ ID N0:3502 -8.4 -21.5 63.9 -12.5 0.1 -8.6 CAGCCTTCACACAGATGTAT

1518 SEQ ID N0:3503 -8.4 -23.9 69.7 -14.8 -0.5 -4.2 TTTTGTTCTGCTTCTTCCAA

1774 5EQ ID N0:3504 -8.4 -23.5 70.3 -15.1 0 -3.6 TTTTTGTCACTGGTCAGTCT

2104 SEQ ID N0:3505 -8.4 -23.8 73.6 -15.4 0 -6.6 ATTTTTTGTCACTGGTCAGT

2106 SEQ ID N0:3506 -8.4 -22.6 70 -14.2 0 -6.4 TGACAATAATTCCATCAAAG

2949 SEQ ID N0:3507 -8.4 -16.7 52.4 -8.3 0 -2.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGGTGAGGTTTCCTAGAGCC

3111 SEQ ID N0:3508 -8.4 -27 79.1 -18.1 -0.1 -3.9 GAGCTGCATGCCGACCACAG

3161 SEQ TD N0:3509 -8.4 -28.8 76.8 -19.5 -0.8 -8.4 CAGTCCCACATGGTCTCTAT

3304 SEQ ID N0:3510 -8.4 -26.6 76.7 -18.2 0 -5.2 CCAATGACCATGACCAACAT

3364 5EQ ID N0:3511 -8.4 -23.2 63.6 -14.3 -O.l -4.5 TGGTGGTTCCATTCCCATCT

3666 SEQ ID N0:3512 -8.4 -28.5 80.1 -19 -1 -6.4 TGGGGTTGTTTATGAATGAC

3744 SEQ ID N0:3513 -8.4 -20.7 62.8 -12.3 0 -3.1 GTTTCAGCTTGTTCACCTTC

3922 SEQ ID N0:3514 -8.4 -25.3 76.4 -16.9 0 -4.3 TCTTCAAAGGCCAATGCACC

4132 SEQ ID N0:3515 -8.4 -24.8 68.9 -14.9 -1.4 -7.7 GGTCTTAAAGCTCTTAATGT

4384 SEQ ID N0:3516 -8.4 -20.4 62.7 -12 0 -5.9 TCCAACAAGAGCATTCACAA

4442 SEQ ID N0:3517 -8.4 -21.1 61.6 -12.1 -0.3 -4.1 GGGAATTGCTCCAACAAGAG

4451 SEQ ID N0:3518 -8.4 -22.3 64 -12.1 -1.8 -5.3 AGGGAATTGCTCCAACAAGA

4452 SEQ ID N0:3519 -8.4 -22.3 64 -12.1 -1.8 -5.2 TATCCATCCAGCCTTTAAAT

4716 SEQ ID N0:3520 -8.4 -23.1 65.8 -14.7 0 -4.3 TTCTTCATATACAGGCTGAA

4772 SEQ ID N0:3521 -8.4 -20.7 63 -12.3 0 -4.1 AAACTAGGGTCATGTATTTG

5124 SEQ ID N0:3522 -8.4 -19.2 59.6 -10.8 0 -4.7 ACACAGAATACTTTTCTATC

5298 SEQ ID N0:3523 -8.4 -18.2 57.8 -8.8 -0.9 -4.4 CAACGCAGGAAGGGACATCA

5417 SEQ ID N0:3524 -8.4 -23.6 66 -15.2 0 -3.6 GGAGGCCGATGTTAAACAAC

5433 SEQ ID N0:3525 -8.4 -22.2 62.8 -13.2 -0.2 -8.l ATGTTCACCACAACCAGGAA

5746 SEQ ID N0:3526 -8.4 -23.5 66.3 -14.4 -0.4 -5.7 TGCACTTTCTTCAGTAGCAA

5798 SEQ ID N0:3527 -8.4 -23 69.2 -13.1 -1.4 -5.5 TGATAGCGGCAGACACCTCC

6171 SEQ ID N0:3528 -8.4 -27.6 75.4 -19.2 0 -5.8 TTTGTCTTTCTCAAACTTTT

6407 SEQ ID N0:3529 -8.4 -19.3 60.8 -10.2 -0.4 -3.6 GTTAAAACAGTCAGTTTGGC

6565 SEQ ID N0:3530 -8.4 -20.5 62.7 -10.5 -1.6 -6.1 TCTTGTATACTGTTTCTTCA

6925 SEQ ID N0:3531 -8.4 -21 66.3 -12.6 0 -6.3 AACCAAGACTTGTGAAGAAT

7599 SEQ ID N0:3532 -8.4 -18.3 55.6 -9.4 -0.2 -5.4 TGACATTCTTTAACCAAGAC

7610 SEQ ID N0:3533 -8.4 -19.1 58.2 -9.7 -0.9 -5 CATGTTGAATTAAAAGTGCA

7716 SEQ ID N0:3534 -8.4 -17.3 54 -8.9 0 -5.2 CATATGATACTCATGTTGAA

7727 SEQ ID N0:3535 -8.4 -18.1 56.7 -8.8 -0.8 -5.2 CAGTATCCAGTGTGTTTCCT

7765 SEQ ID N0:3536 -8.4 -25.8 76.6 -17.4 0 -3.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TATATTTAAACAAGTTTTAG

7828 SEQ ID N0:3537 -8.4 -13.3 46.6 -4.9 0 -5.5 TTTTAGGAAATTATCCATTG

7999 SEQ ID N0:3538 -8.4 -17.4 54.8 -7.5 -1.4 -6.4 TTTATTTTAGGAAATTATCC

8003 SEQ ID N0:3539 -8.4 -16.5 53.4 -6.9 -1.1 -5.9 ATGAATAAAAGGTTATATTG

8140 SEQ ID N0:3540 -8.4 -13.5 46.6 -4.5 -0.3 -3 TGACGTAGCTCACCATTCTT

8427 SEQ ID N0:3541 -8.4 -24.7 71.1 -16.3 0 -5.3 AACATGTGAACTTGATCCTT

8694 SEQ ID N0:3542 -8.4 -20.5 61.1 -12.1 0 -7 ATTTATTAGCCACCAAGTAA

8837 SEQ ID N0:3543 -8.4 -20.6 61.1 -12.2 0 -3.2 TTACAACTGGAGTTTTCCAC

9002 SEQ ID N0:3544 -8.4 -21.7 64.8 -11.2 -2.1 -5.6 TTTGCTCCTTTCCCAGTAAG

310 SEQ ID N0:3545 -8.3 -25.7 73.5 -17.4 0 -3.6 TTCTGTGAATTATCTCATTT

358 SEQ ID N0:3546 -8.3 -18.9 59.9 -10.6 0.9 -3.2 TCCATAAATAAATGGAAGGT

650 SEQ ID N0:3547 -8.3 -17.2 53.2 -6.8 -2.1 -7.5 ATGTCTCCATAAATAAATGG

655 SEQ ID N0:3548 -8.3 -17.4 54.1 -7.8 -1.2 -5.7 TATAAAAGTTTTCTTATTGA

725 SEQ TD N0:3549 -8.3 -14.6 49.5 -5.6 -0.5 -6.3 ATAAATACACAGTTGGTCAA

886 SEQ ID N0:3550 -8.3 -17.9 55.9 -9.6 0 -3.6 TTCTTTACCGACTGGATCAG

1189 SEQ ID N0:3551 -8.3 -22.9 66.7 -13.7 -0.7 -5.3 CGCTTGGGGGCCACTGCAAA

1302 SEQ ID N0:3552 -8.3 -29.1 76.1 -18.9 -1.9 -6.9 ATGGCCACCACAGCCAGGAT

1726 SEQ ID N0:3553 -8.3 -29.8 79.3 -18.7 -2.8 -9.1 TTTGTTCTGCTTCTTCCAAG

1773 SEQ ID N0:3554 -8.3 -23.4 70.2 -15.1 0 -3.6 GTTTCCATCCATGGAGAAAA

2084 SEQ ID N0:3555 -8.3 -21.9 63.3 -11.4 -1.7 -12.3 AGGGGAGCAGAATTTTTTGT

2117 SEQ ID N0:3556 -8.3 -22.3 66.9 -14 0 -4.1 TGCGTCGCTCTCCATGTCTG

2316 SEQ ID N0:3557 -8.3 -28.6 79.6 -19.7 -0.3 -6.9 CTGGCCTGACTAACGTTACT

2338 SEQ ID N0:3558 -8.3 -24.6 69.1 -15.8 0 -7.9 TTTACTTTTAACCATGCATC

2701 SEQ ID N0:3559 -8.3 -20.1 60.9 -11.8 0 -6.6 CATCAAAGATATTCCAGCCT

2937 SEQ ID N0:3560 -8.3 -22.6 65 -14.3 0,1 -3.5 TGATCTTAATTAGCATATTT

3075 SEQ TD N0:3561 -8.3 -17.2 55.3 -8.9 0 -4.9 TTTCCTAGAGCCCCCACAGA

3103 5EQ ID N0:3562 -8.3 -29.8 79.9 -21.5 0 -3.8 TTTTCATCGATTACGTATTT

3715 SEQ ID N0:3563 -8.3 -19.1 58.6 -9.9 -0.7 -6.1 CTGTAGCAGGTTTTTCGAAG

4054 SEQ ID N0:3564 -8.3 -22.2 66.4 -13.9 0 -6.8 TAATGTCCGTAATGATTTGA

4370 SEQ ID N0:3565 -8.3 -19 57.6 -10.7 0 -2.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Tnter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGCTCCAACAAGAGCATTCA

4445 SEQ ID N0:3566 -8.3 -23.6 67.7 -12.1 -3.2 -9.1 GACAGACCAACAGCACATTC

4479 SEQ ID N0:3567 -8.3 -23.1 66.6 -14.8 0 -4.1 GAACTTGCCAGCAAACAAAT

4535 SEQ ID N0:3568 -8.3 -20.2 58.3 -11.9 0 -7.4 TACCCGTTGTCATGTTAACA

4563 SEQ ID N0:3569 -8.3 -23.5 67.1 -14.7 0.2 -8.4 CTTCATTGCATTGTAATATT

4943 SEQ ID N0:3570 -8.3 -18.8 58.6 -10.5 0 -6.2 GGGCGAGGTATGGGTTTCTG

4984 SEQ ID N0:3571 -8.3 -26.8 76.8 -18.5 0 -4 AGATGAGGATCATGATGCTG

5061 SEQ ID N0:3572 -8.3 -21.4 64.3 -l1 -2.1 -8.5 ACATGTCATCAATTCCAGCT

5520 SEQ ID N0:3573 -8.3 -23.5 68.9 -15.2 0 -6.1 TTCTTCAGTAGCAACACTGA

5792 SEQ ID N0:3574 -8.3 -21.9 66.3 -11.6 -2 -7.8 GCTGAATGATAGCGGCAGAC

6177 SEQ ID N0:3575 -8.3 -24 68.1 -14.4 -1.2 -6.6 CCTCTTTGTTATAGTTACTT

6243 SEQ ID N0:3576 -8.3 -21.3 65.9 -13 0 -2.5 GTAGAGGAACTCCCATCTGT

6343 SEQ ID N0:3577 -8.3 -25.3 73.3 -14.6 -2.4 -6.7 GGTAGAGGAACTCCCATCTG

6344 SEQ ID N0:3578 -8.3 -25.3 72.5 -14.6 -2.4 -7.3 GTCTTTCTCAAACTTTTCCT

6404 SEQ ID N0:3579 -8.3 -22.4 67.6 -14.1 0 -1.9 TGTCTTTCTCAAACTTTTCC

6405 SEQ ID N0:3580 -8.3 -21.5 65.5 -13.2 0 -2.4 ACTTTACCTTCATAGGCTGT

6511 SEQ ID N0:3581 -8.3 -24.1 71.4 -14.6 -1.1 -4.1 CTTGAAGTCCAGTTGACACA

6534 SEQ ID N0:3582 -8.3 -22.9 67.4 -12.8 -1.8 -5.7 CATTATGAATAAACTATGAA

6872 SEQ ID N0:3583 -8.3 -13.4 46 -5.1 0 -3.5 GTAGCCATTACAATTCTTTT

7013 SEQ ID N0:3584 -8.3 -21.1 63.8 -12.3 -0.1 -3.2 TTAGATTACTTTTTGGAAAG

7234 SEQ ID N0:3585 -8.3 -16.1 52.6 -6.3 -1.4 -4.9 GAATGGATTTATTAGATTAC

7245 SEQ ID N0:3586 -8.3 -16 52.5 -7.7 0 -2.4 ATTAGTGATAGCCTTTAAAC

7538 SEQ ID N0:3587 -8.3 -18.7 58 -10.4 0 -4.2 ATGGATTGAGTAAAATAGAG

7581 SEQ ID N0:3588 -8.3 -15.6 51.1 -7.3 0 -2.3 CCAATATGTGACATTCTTTA

7618 SEQ ID N0:3589 -8.3 -19.7 59.9 -11.4 0 -3.9 TAATGGACAGAGCAGGTTGA

7651 SEQ ID N0:3590 -8.3 -21.9 65.2 -13.6 0 -4.1 AGCTTCAAATTATTCTGCTT

7675 SEQ ID N0:3591 -8.3 -20.5 62.6 -12.2 0 -4.3 TATTTATATTTAAACAAGTT

7832 SEQ ID N0:3592 -8.3 -13.3 46.6 -4.4 -0.3 -4.9 TTATTTATATTTAAACAAGT

7833 SEQ ID N0:3593 -8.3 -13.3 46.6 -4.4 -0.3 -4.9 CAAAAGTTTGGAATTGTGAT

7963 SEQ ID N0:3594 -8.3 -17 53.5 -8.7 0 -4.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTAACATTAAACTTTTGGTT

8340 SEQ ID N0:3595 -8.3 -16.3 52.6 -7.4 -0.3 -3.9 TAGCAATTCCCTGCATGCAG

8399 SEQ ID N0:3596 -8.3 -25.9 72.4 -14.7 -1.9 -13.9 TAGCAATAGCAATTCCCTGC

8405 SEQ ID N0:3597 -8.3 -24.2 68.6 -14.2 -1.7 -6.2 ATTACAACTGGAGTTTTCCA

9003 SEQ ID N0:3598 -8.3 -21.5 64.3 -11.2 -2 -5.5 GGGAGAATTATGAAATACAT

9054 SEQ ID N0:3599 -8.3 -16.5 52.4 -7.7 -0.2 -3.8 CAATTTTTTTTCTTTCTTTC

64 SEQ ID N0:3600 -8.2 -18.1 58.6 -9.9 0 -2.6 GATGGTTAGGGTATAACGTG

154 SEQ TD N0:3601 -8.2 -21.3 63.6 -11.1 -2 -5.3 AAAGCTCCAGGTCCCTTCTG

373 SEQ TD N0:3602 -8.2 -27.5 76.7 -19.3 0 -5 ACAGTGCCTGTGCCATCTTT

468 SEQ ID N0:3603 -8.2 -28 79.4 -18.7 -1 -7.8 TCTTTCCAGCTTCCAAGTCA

630 SEQ ID N0:3604 -8.2 -26.1 75.9 -17.9 0 -4.5 GTCTCCATAAATAAATGGAA

653 SEQ ID N0:3605 -8.2 -17.3 53.7 -6.8 -2.3 -7.8 CAAAATCTTGATAGCAATTT

827 SEQ ID N0:3606 -8.2 -16.3 52 -7.5 -0.3 -0.5 TAAATACACAGTTGGTCAAA

885 SEQ ID N0:3607 -8.2 -17.2 54.1 -9 0 -3.8 AGCAAACACGCTCAGACAGA

1238 SEQ ID N0:3608 -8.2 -22.9 65.3 -14 -0.4 -4.3 CATTTATTCCTCAGATTGCC

1282 SEQ ID N0:3609 -8.2 -23.6 69 -15.4 0 -3 AAACATAAAAGTGACTGTCA

1434 SEQ ID N0:3610 -8.2 -16.3 52 -7.5 0 -8.6 CCAGCCTTCACACAGATGTA

1519 SEQ ID N0:3611 -8.2 -25.9 73.4 -17 -0.5 -4.2 GTGCGGCACAAACAGTGAGT

2297 SEQ ID N0:3612 -8.2 -24.9 70.3 -14.8 -1.5 -11.7 TGTGCGGCACAAACAGTGAG

2298 SEQ ID N0:3613 -8.2 -23.7 67 -13 -l.5 -13 CTCTCCATGTCTGTGCGGCA

2309 SEQ ID N0:3614 -8.2 -29.3 81.9 -20.6 0 -7.7 CCAGATCAAGAACACATTGG

2672 SEQ ID N0:3615 -8.2 -20.7 60.7 -12.5 0 -5.4 TCACAAGATGTTTTACTTTT

2712 SEQ ID N0:3616 -8.2 -18.5 58.5 -10.3 0 -3 ACTCTAAGCAGTCTGAATGA

3025 SEQ ID N0:3617 -8.2 -20.9 63.6 -12.7 0 -6.3 AACTCTAAGCAGTCTGAATG

3026 SEQ ID N0:3618 -8.2 -19.6 60.1 -11.4 0 -5.8 ATGATCTTAATTAGCATATT

3076 SEQ ID N0:3619 -8.2 -17.1 55 -8.9 0 -4.9 ACAATAAGGCACATGGTTTG

3340 SEQ ID N0:3620 -8.2 -20.4 60.9 -12.2 0 -5.2 ATTTTCATCGATTACGTATT

3716 SEQ ID N0:3621 -8.2 -19 58.3 -9.9 -0.7 -6.3 GATAGTCTTTCGCTGTTCAA

4160 SEQ ID N0:3622 -8.2 -22.6 68 -14.4 0 -3.1 TCTTAAAGCTCTTAATGTCC

4382 SEQ ID N0:3623 -8.2 -20.4 62.2 -12.2 0 -5.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Tnter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AACCACCCTCATGCCTTCAA

4424 SEQ ID N0:3624 -8.2 -27.6 73.5 -19.4 0 -4.4 AGACCAACAGCACATTCATG

4476 SEQ ID N0:3625 -8.2 -22.3 64.6 -14.1 0 -4,1 CTCCAAACTTCTTTTTCTGC

4890 SEQ ID N0:3626 -8.2 -22.6 66.9 -14.4 0 -2.6 ACCTCCAAACTTCTTTTTCT

4892 SEQ ID N0:3627 -8.2 -23 67.1 -14.8 0 -1.8 GCATTGTAATATTTTTTCTG

4936 SEQ TD N0:3628 -8.2 -18.3 58 -10.1 0 -4.2 TTCTTGGATCCAAGTTTCTT

4960 SEQ ID N0:3629 -8.2 -22.5 67.9 -10.6 -1.3 -15.5 GATGCTGATATCAAAGACTT

5048 SEQ ID N0:3630 -8.2 -19.1 58.5 -10.4 0 -7.5 CGAGCTTCAGCACAAATTCT

5184 SEQ ID N0:3631 -8.2 -22.7 65.5 -13.6 -0.7 -6.6 AAGTAGTAGTGTCTGAGGGA

5206 SEQ ID N0:3632 -8.2 -22.4 69.6 -14.2 0 -2.9 TCCTTTGATCAGACGTAGGA

5363 SEQ ID N0:3633 -8.2 -23.5 68.7 -14.8 -0.1 -7.5 CATGTCATCAATTCCAGCTT

5519 SEQ ID N0:3634 -8.2 -23.4 68.7 -15.2 0 -4.5 CACAACCAGGAAGGATATGA

5738 SEQ ID N0:3635 -8.2 -21 61 -12.8 0.1 -3.7 AATCAGAGAGTTTAGAGAAC

5898 SEQ ID N0:3636 -8.2 -17.4 56.1 -8.6 -0.3 -3.2 GCAGATCCATGGCAATAAGC

5970 SEQ ID N0:3637 -8.2 -24 68.2 -15.1 -0.5 -8 TGGTAGAGGAACTCCCATCT

6345 5EQ TD N0:3638 -8.2 -25.3 72.5 -14.6 -2.5 -7.9 TGTCTGGTTTTGTTACACTA

6384 SEQ ID N0:3639 -8.2 -21.9 67.7 -13.7 0 -2.7 CACTTTGCACAACTATCCCT

6736 SEQ ID N0:3640 -8.2 -24.8 69.6 -16.6 0 -4.7 AGAATGGATGCAAGGACTGT

6786 SEQ ID N0:3641 -8.2 -21.6 63.7 -13.4 0 -5.6 TTCAACGTAAACCTCATTTA

6909 SEQ ID N0:3642 -8.2 -19.1 57.4 -10.4 -0.2 -5.3 AAGTAACTGAAACTGTAGCC

7027 SEQ ID N0:3643 -8.2 -19.6 59.1 -11.4 0 -3.3 AAATTAAATATAGAGCTCTG

7204 SEQ ID N0:3644 -8.2 -15 49.8 -5.8 0 -10 TACCATATGATACTCATGTT "

7730 SEQ ID N0:3645 -8.2 -20.1 61.2 -11 -0.8 -6 AATTTTCTTATGTACAAAAT

7884 SEQ ID N0:3646 -8.2 -14.4 48.6 -5.6 -0.3 -6.8 ATATCATGTTAGGTAATTGC

8300 SEQ ID N0:3647 -8.2 -19.1 60 -10.9 0 -4.7 GAAAGAAGATGAGTTTAGAT

8793 SEQ ID N0:3648 -8.2 -16 52.2 -7.8 0 -2.2 TTGACCTCACATAAATCCAA

8.963 SEQ ID N0:3649 -8.2 -20.5 59.8 -12.3 0 -2.9 TATTATGGGAGAATTATGAA

9060 SEQ ID N0:3650 -8.2 -16.1 52 -7.9 0 -3.2 CTTATATAATTTATTATGGG

9071 SEQ ID N0:3651 -8.2 -15.5 51.3 -6.7 -0.3 -5.3 GTCTTCCTCTGGCAGCTGTT

135 SEQ ID N0:3652 -8.1 -28.9 85.1 -20.1 0 -8.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CAGCATAACAAAGCCCAGCA

179 SEQ ID N0:3653 -8.1 -24.7 67.6 -15.7 -0.7 -5 TCCAGGTCCTCCAGGGGCTC

694 SEQ TD N0:3654 -8.1 -32.6 91 -23.2 -1.2 -8.4 TTTATTCATTACTATAAAAG

737 SEQ ID N0:3655 -8.1 -13.3 46.6 -5.2 0 -3.2 TCTTTGTCCAGTCAGGAGGG

918 SEQ ID N0:3656 -8.1 -26.3 78.3 -16.8 -1.3 -5 CTCAACGCTGAGACATTGCC

1099 SEQ TD N0:3657 -8.1 -25 69.6 -15.4 -1.4 -5.8 CTGCAGCCCAATGAGAGCAA

1253 SEQ ID N0:3658 -8.1 -26.2 71.6 -17.4 -0.5 -7.2 GTCATCTCCAATGTAATCCT

1418 SEQ ID N0:3659 -8.l -23.5 68.7 -14.8 -0.3 -3 TTGCTTTTTAAGCTGTTCGA

1817 SEQ ID N0:3660 -8.l -22 65.6 -11.3 -2.6 -9 AGGTGCTCTCTCTGTCTTCT

1987 SEQ ID N0:3661 -8.1 -26.9 83.3 -18.8 0 -3.6 GCTTCTTTTGACGCTGTCTT

2057 SEQ ID N0:3662 -8.1 -25.3 74.2 -15.8 -1.3 -7.6 TGCTGGAAGCCCTGGCACCA

2372 SEQ ID N0:3663 -8.1 -31.1 81.5 -20.8 -2.2 -10.4 ACAGTGCTGTGCATCTTCCC

2395 SEQ ID N0:3664 -8.1 -28.3 80.9 -19.1 -1 -8.5 TTCTGACTTCCGTTTCAGTG

2499 SEQ ID N0:3665 -8.1 -24.1 71.3 -14.8 -1.1 -5.7 ACACTACTGAATTGCTCAGT

2824 SEQ ID N0:3666 -8.1 -21.8 65.4 -11.4 -2.3 -7.5 CACACTACTGAATTGCTCAG

2825 SEQ ID N0:3667 -8.1 -21.3 63.4 -11.4 -1.8 -6.2 CTGCAGATTATTCATTTCAT

3467 SEQ ID N0:3668 -8.1 -20.3 62.5 '12.2 0.6 -7.6 AATCATTTTCATCGATTACG

3720 SEQ ID N0:3669 -8.1 -18.4 56.3 -9.6 -0.4 -6.3 CTTCAGATGAGCTGGTTGCA

3873 5EQ ID N0:3670 -8.1 -25 73.5 -15.3 -1.5 -6.8 AACTTTTTAATACACCCTTC

3982 SEQ ID N0:3671 -8.1 -20 60 -11.9 0 -2.5 AAGTCTCAAACCAGTTGTGC

4083 SEQ ID N0:3672 -8.1 -22.7 67.2 -13.9 -0.4 -3.4 GTCTTTCGCTGTTCAATGTA

4156 SEQ ID N0:3673 -8.1 -23.2 69.6 -15.1 0 -3.1 ATCTAGCCAGCACCAGGCAT

4280 SEQ ID N0:3674 -8.1 -28.6 79.3 -18.6 -1.9 -7.4 CCTCATGCCTTCAAACCGGG

4418 5EQ ID N0:3675 -8.1 -27.9 73.3 -19.8 0 -7.l AACTTGCCAGCAAACAAATT

4534 SEQ ID N0:3676 -8.1 -19.7 57.5 -11.6 0 -7.4 CAGTGGTAGAACTTGCCAGC

4543 SEQ ID N0:3677 -8.1 -25.4 73.3 -16 -1.2 -4.8 AAATTCTCCAGTGAACAGAA

5171 SEQ ID N0:3678 -8.1 -19 57.6 -10.9 0 -4.5 AATACTTTTCTATCATCTCA

5292 SEQ ID N0:3679 -8.1 -18.9 59.9 -10.8 0 -1.4 AGGAGGCCGATGTTAAACAA

5434 SEQ ID N0:3680 -8.1 -22 62.5 -13.2 -0.5 -8.1 CTTTTCCCAAACCTCATAGA

5849 SEQ ID N0:3681 -8.1 -23.4 66.4 -15.3 0 -2.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTGGTAGAGGAACTCCCATC

6346 5EQ ID N0:3682 -8.1 -25.6 73.9 -14.6 -2.9 -8.2 TTGAAGTCCAGTTGACACAT

6533 SEQ ID N0:3683 -8.1 -22 65.4 -12.8 -1 -5.7 ATCATTTGAGAGACAGGGTT

6944 SEQ ID N0:3684 -8.1 -21.7 66.1 -13.6 0 -4 AGTAACTGAAACTGTAGCCA

7026 SEQ ID N0:3685 -8.1 -21 62.2 -12.9 0 -3.3 CATAACTGTTCAGATAGAAA

7098 SEQ ID N0:3686 -8.1 -16.7 53.4 -7.9 -0.5 -5 CATTAGTGATAGCCTTTAAA

7539 SEQ ID N0:3687 -8.1 -19.2 58.8 -11.1 0 -4 ACTACAGTAAATAGTTTTGT

8368 SEQ ID NO:3688 -8.1 -17.6 56.6 -9.5 0 -3.6 CTTTTTAGCAATAGCAATTC

8410 SEQ ID N0:3689 -8.1 -18.8 58.6 -9 -l.7 -4.6 GTAGCTCACCATTCTTTTTA

8423 SEQ ID N0:3690 -8.1 -23.1 69.8 -15 0 -4.8 AAAACCCCAGAGCCCAATAA

8491 SEQ TD N0:3691 -8.1 -24.2 64.1 -16.1 0 -3.2 ATTAATTATATATATTTTAA

8538 SEQ ID NO:3692 -8.1 -11.3 42.5 -3.2 0 -6.8 ATTGTAGGTGGTTACTACTA

8737 SEQ ID N0:3693 -8.1 -21.2 65.9 -11.5 -1.6 -5.2 GCATCAATTATATTGAAAGA

8807 SEQ ID N0:3694 -8.1 -16.3 52.3 -6.2 -2 -7.9 CATTTATTAGCCACCAAGTA

8838 SEQ ID NO:3695 -8.1 -22 64.3 -13.9 0 -3.2 TGACCTCACATAAATCCAAG

8962 SEQ ID N0:3696 -8.1 -20.4 59.7 -12.3 0 -2.6 TTTTGACCTCACATAAATCC

8965 SEQ ID N0:3697 -8.1 -20.7 61.2 -12.6 0 -2.9 ACAGCATAACAAAGCCCAGC

180 SEQ ID N0:3698 -8 -24.2 67.1 -15.7 -0.2 -4.1 TAGCTTCTTGCATACGAATT

424 SEQ ID N0:3699 -8 -21.1 62.7 -11.5 -1.5 -5.2 GCGGAAGCTTTCAGGTCCTG

497 SEQ ID N0:3700 -8 -27.4 76.8 -17.7 -1.6 -10.7 CCAAGTCACTATTTGGCTTT

618 SEQ ID N0:3701 -8 -23.4 68.5 -13.8 -1.5 -5 CTCTTGCCAAGATTTTTATA

981 SEQ TD N0:3702 -8 -20.5 62.1 -11.7 -0.6 -6.3 CAGGGCCCCCACAATGGTCT ' 1172 SEQ ID N0:3703 -8 -31.5 82.2 -21.7 -0.7 -11.8 ATTGCCCATGAACAGCTGCA

1268 SEQ ID N0:3704 -8 -26.6 72.8 -17.9 -0.2 -8.9 TGTATCCTTCTGGACACTGG

1503 SEQ ID N0:3705 -8 -24.6 71.9 -16.6 1.5 -5.9 ACTGGTAAAGATTTTCCCAA

1623 SEQ ID N0:3706 -8 -21.1 61.8 -13.1 0 -4.8 TCCATTCTTTAGCACCTTTG

1950 SEQ ID N0:3707 -8 -24.2 70.6 -16.2 0 -4.l GGAAGCTGCTTCTTTTGACG

2064 SEQ ID N0:3708 -8 -23.8 68.7 -13.2 -1.8 -13.3 AGTCTGTTTCCATCCATGGA

2089 SEQ ID N0:3709 -8 -25.9 75.5 -16.3 -1.5 -10.1 GATCTTAATTAGCATATTTA

3074 SEQ ID N0:3710 -8 -16.9 54.8 -8.9 0 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CATCATCAGTAGCAGCAAGG

3444 SEQ ID N0:3711 -8 -23.3 69.1 -15.3 0 -5.4 TTTCACATAATCAATTCCCT

3509 SEQ ID N0:3712 -8 -2l 62 -13 0 -2.5 AAACACTCCCGCATCTTATT

3529 SEQ ID N0:3713 -8 -23.5 65.7 -15.5 0 -3.6 CTTTCTAAAAAAGGCTTTTT

3551 SEQ ID N0:3714 -8 -16.9 53.5 -7.6 -1.2 -8.2 CCAGTATTATTGGACATGCA

3613 SEQ ID N0:3715 -8 -22.7 66.4 -14.7 0 -5.2 CCATCTCTAAGATAATTAAG

3652 SEQ ID N0:3716 -8 -17 53.9 -8.3 -0.5 -4.9 GGGTTCAGTTTCAGCTTGTT

3929 SEQ TD N0:3717 -8 -25.4 77.7 -17.4 0 -4.5 AAAGTCTCAAACCAGTTGTG

4084 SEQ ID N0:3718 -8 -20.2 60.9 -11.5 -0.4 -3.4 AGGCCAATGCACCACTACTG

4125 SEQ ID N0:3719 -8 -26.3 72.1 -16.8 -1.4 -7.7 GTTCAATGTATATATCTTCA

4146 SEQ ID N0:3720 -8 -18.5 59.2 -10.5 0 -3.9 AAGAGCATTGGCTACCAGGC

4328 5EQ ID N0:3721 -8 -26 73.6 -16.2 -1.8 -8.4 TGGTAGAACTTGCCAGCAAA

4540 SEQ ID N0:3722 -8 -22.8 65.3 -13.9 -0.8 -7.5 CAGGCTGAAGTTTAACATCT

4761 SEQ ID N0:3723 -8 -21.1 63.4 -13.1 0 -4.6 TCCAGCCTATAGTGAAGTAG

5220 5EQ ID N0:3724 -8 -23.2 68.7 -15.2 0 -5.1 AAGATGTTCCAGCCTATAGT

5227 SEQ ID N0:3725 -8 -23.6 69.4 -15.6 0 -5.1 ATCATCAAAGCAAAGAGCAG

5401 SEQ ID N0:3726 -8 -l9 57.7 -9.4 -1.6 -4.4 CAGAGGTTGTAATTTGGAAC

5577 SEQ ID N0:3727 -8 -19.5 60 -11.5 0 -3 ACCACAACCAGGAAGGATAT

5740 SEQ ID N0:3728 -8 -22.6 63.9 -13.8 -0.6 -4.3 TTTCTTCAGTAGCAACACTG

5793 SEQ ID N0:3729 -8 -21.4 65.3 -12.3 -1 -5.8 AGGGGCTCTGCACTTTCTTC

5806 SEQ ID N0:3730 -8 -27.2 80.5 -19.2 4.6 -4.8 CGCATCGGGATCAAACTTTT

5864 SEQ ID N0:3731 -8 -22.6 63.7 -14.6 0 -5.3 CTTGTCTGGTTTTGTTACAC

6386 SEQ ID N0:3732 -8 -22.3 68.7 -14.3 0 -2.9 TCTTGAAGTCCAGTTGACAC

6535 SEQ ID N0:3733 -8 -22.6 67.8 -12.8 -1.8 -5.1 ATGGATGCAAGGACTGTACT

6783 SEQ ID N0:3734 -8 -22.5 66.2 -14.5 0 -5.1 AAAATATGGCAGATATGGAA

6815 SEQ ID N0:3735 -8 -16.7 52.4 -7.3 -1.3 -5.2 GACCTAAATTAAATATAGAG

7209 SEQ ID NO:3736 -8 -14.4 48.1 -6.4 0 -3.2 AGCAAAATATGAAGAATAGG

7798 SEQ ID N0:3737 -8 -14.8 48.7 -6.8 0 -4.1 ATTGTGATGTCATCAACCTG

7909 SEQ ID N0:3738 -8 -21.7 64.7 -12.6 -1 -7.2 ACGTAGCTCACCATTCTTTT

8425 SEQ ID N0:3739 -8 -24.3 70.7 -16.3 0 -4.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATTATATATATTTTAAACTG

8534 SEQ ID N0:3740 -8 -12.6 45.1 -4.6 0 -5.2 GTTTTGACCTCACATAAATC

8966 SEQ ID N0:3741 -8 -19.9 60.5 -11.9 0 -2.9 CAGGTCCCTTCTGTGAATTA

366 SEQ ID N0:3742 -7.9 -24.7 71.6 -16.8 0 -4.2 GCTTTCTCTTCTGCAGCACG

553 SEQ ID N0:3743 -7.9 -26.9 77.5 -18.3 -0.4 -8.3 GTTCTCATCATCATTATCTT

593 SEQ ID N0:3744 -7.9 -21.1 66.4 -13.2 0 -0.9 CAGTCAGGAGGGTTGCTCAA

910 SEQ ID N0:3745 -7.9 -25.6 75.3 -15.7 -2 -5 CAAAACCCTCTTGCCAAGAT

988 SEQ ID N0:3746 -7.9 -23.5 64.6 -14.8 -0.6 -6.3 GGGGCCACTGCAAACATTTA

1296 SEQ ID N0:3747 -7.9 -25.1 69.5 -15.7 -1.4 -7.6 ATTTCCACAGAGTAAAGGGT

1469 SEQ ID N0:3748 -7.9 -21.9 65.2 -14 0 -3.6 CTTTTTGTTCTGCTTCTTCC

1776 SEQ ID N0:3749 -7.9 -24.5 74.1 -16.6 0 -3.6 CTCTCCTAACCCACCTACTC

1892 SEQ ID N0:3750 -7.9 -27.7 76.1 -19.8 0 -0.3 GGATACTCAAGAGAGACTGA

2139 SEQ ID N0:3751 -7.9 -20.5 62.4 -11.8 -0.6 -4.5 CGTCTTGGGGAAAACAGGGA

2164 SEQ ID N0:3752 -7.9 -23.4 65.8 -15.5 0 -2.7 GAAAATCCCAGTAAAGACCA

2861 SEQ ID N0:3753 -7.9 -20.6 59.1 -12.7 0 -2.1 GAATGATCGCAGTACAGACA

3011 SEQ ID N0:3754 -7.9 -21.5 63.2 -12.9 -0.4 -5.7 CCATACAGTCCCACATGGTC

3309 SEQ ID N0:3755 -7.9 -27.3 76.3 -18.7 -0.5 -5.2 TATTTTTCACATAATCAATT

3513 SEQ ID N0:3756 -7.9 -15.6 51.4 -7.7 0 -2.3 TGGCACTGTGACGGTGAGGC

3764 SEQ ID N0:3757 -7.9 -27.6 77.3 -17.9 -1.8 -7.6 AGTAAAACAAGCTTCCGGTT

3962 SEQ ID N0:3758 -7.9 -21.5 62.2 -13.6 0 -6.6 TCTTCCCTTTGCCTTCTTCT

4023 SEQ TD N0:3759 -7.9 -28.4 81.3 -20.5 0 -3 ATCAAGAAATCTAGCCAGCA

4288 SEQ ID N0:3760 -7.9 -21.6 63.3 -13.7 0 -4.6 CCAAGAGCATTGGCTACCAG

4330 SEQ ID N0:3761 -7.9 -25.7 71.5 -16.2 -1.6 -7.2 TACAGATTTTCTTCATATAC

4780 SEQ ID N0:3762 -7.9 -17.6 57 -9.7 0 -2.6 TATTTTTTCTGTTCCTCTGT

4927 SEQ ID N0:3763 -7.9 -22.6 69.9 -14.7 0 -0.7 AAAGACCATTCCTTGGAATT

5012 SEQ ID N0:3764 -7.9 -20.8 60.6 -11.2 -1.7 -8.4 TGGTGACCATGTTGAGGCAG

5079 SEQ ID N0:3765 -7.9 -25.5 73.3 -17.1 -0.2 -5.5 TGAAGTAGTAGTGTCTGAGG

5208 SEQ ID N0:3766 -7.9 -21.2 66.5 -13.3 0 -2.9 GTGAAGTAGTAGTGTCTGAG

5209 SEQ ID N0:3767 -7.9 -21.2 67.3 -13.3 0 -2.9 AGTGAAGTAGTAGTGTCTGA

5210 SEQ ID N0:3768 -7,g -21.2 67.3 -13.3 0 -2.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATGTCATCAATTCCAGCTTC

5518 SEQ ID N0:3769 -7.9 -23.1 69.1 -15.2 0 -4.5 CCAGGGTGAATTGTGTCAGG

5653 SEQ ID N0:3770 -7.9 -25 72.4 -17.1 0 -3.3 TGTACATGTTCACCACAACC

5751 SEQ TD NO:3771 -7.9 -23.5 67.4 -14.9 -0.4 -7.1 AGCAACACTGAAGTTCTCCA

5783 SEQ ID N0:3772 -7.9 -23.4 68.1 -15.5 0 -6.3 CTTTCTTCAGTAGCAACACT

5794 SEQ ID N0:3773 -7.9 -22.3 67.5 -13.7 -0.5 -0.8 GACCATGGGCAGATCCATGG

5978 SEQ ID N0:3774 -7.9 -27.3 75 -14.3 -5.1 -12.8 TTTTTAACCTTTGCTTTAAA

6216 SEQ ID N0:3775 -7.9 -17.9 55.7 -10 0 -4 TGCTTTCTTTTTCTGGTTTG

6423 SEQ ID N0:3776 -7.9 -22.5 69.3 -14.6 0 -3.6 TTAAAACAGTCAGTTTGGCA

6564 SEQ ID N0:3777 -7.9 -20 60.8 -10.5 -1.6 -7.7 TCTGATCATTTGAGAGACAG

6948 SEQ ID N0:3778 -7.9 -19.9 61.9 -11.5 -0.1 -6.2 TCTAGAAAATGGAAGTAACT

7039 SEQ ID N0:3779 -7.9 -15.9 51.4 -8 0 -5.2 AATTAAAGCCATCTAGAAAA

7050 SEQ ID N0:3780 -7.9 -15.7 50.2 -7.8 0 -6.2 AAATTAAAGCCATCTAGAAA

7051 SEQ ID N0:3781 -7.9 -15.7 50.2 -7.8 0 -6.2 CACATAACTGTTCAGATAGA

7100 SEQ ID N0:3782 -7.9 -19 59 -10.6 -0.2 -5.6 TATTAGAGGAGACTTTACAG

7121 SEQ ID N0:3783 -7.9 -18.4 58.5 -9.6 -0.7 -5.5 CATTTGACCTAAATTAAATA

7214 SEQ ID N0:3784 -7.9 -15 49 -6.5 -0.3 -5 TTTGGAAAGCATTTGACCTA

7223 SEQ ID N0:3785 -7.9 -20.7 61.2 -12 -0.6 -4.1 GCAATACTTTAGATATATTT

7270 SEQ.ID N0:3786 -7.9 -16.7 54 -8.8 0 -4.3 AAAGCAATACTTTAGATATA

7273 SEQ ID N0:3787 -7.9 -15.1 50 -6.l -1 -4.7 AATGGATTGAGTAAAATAGA

7582 SEQ ID N0:3788 -7.9 -14.9 49.3 -7 0 -2.1 CACCAATATGTGACATTCTT

7620 SEQ ID N0:3789 -7.9 -20.8 61.9 -12.9 0 -4 TTCATTCTATCACCAATATG

7630 SEQ ID N0:3790 -7.9 -19.3 59.3 -11.4 0 -2.7 TAGACATATTTTTAGCAAAA

7811 SEQ ID N0:3791 -7.9 -15.4 50.6 -6.8 -0.4 -5 TTTAAACAAGTTTTAGACAT

7824 SEQ ID N0:3792 -7.9 -15.4 50.8 -6.8 -0.4 -5.5 GCAATAGCAATTCCCTGCAT

8403 SEQ ID N0:3793 -7.9 -25.2 70 -15.6 -1.7 -6.8 TTTTGGCTCAATAATGACGT

8441 SEQ ID N0:3794 -7.9 -20.4 60.6 -11.8 -0.5 -5.3 ACAAAGCTGCTTTTAATTTG

8657 SEQ ID N0:3795 -7.9 -18.4 56.8 -9 -0.5 -10.9 CTATTGTAGGTGGTTACTAC

g739 SEQ ID N0:3796 -7.9 -21.2 65.9 -12.8 -0.1 -4.3 CATAGGAGTTTATATAATGC

8880 SEQ ID N0:3797 -7.9 -17.8 56.6 -9.9 0 -5.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTGGTTTTGACCTCACATAA

8969 SEQ ID N0:3798 -7.9 -21.5 63.9 -11.3 -2.3 -5 AGTTTGGTTTTGACCTCACA

8972 5EQ ID N0:3799 -7.9 -23.8 70.7 -13.6 -2.3 -5.7 TTTTTTTTTTTTTTTTCCAC

9091 SEQ ID N0:3800 -7.9 -18.8 59.8 -10.9 0 0 AGCTGTTCAGCTTTTTTTTT

122 SEQ ID N0:3801 -7.8 -22.6 69.8 -13 -1.7 -10.8 GGCTTCAGTTTTCTTGCTCC

232 SEQ ID N0:3802 -7.8 -27.1 80.5 -18.6 -0.4 -3.7 GAATCCTTGACAGGTATCTC

278 SEQ ID N0:3803 -7.8 -22.6 67.8 -13.8 -0.9 -3.6 TCTTACAATATCCCTAGAAG

336 SEQ ID N0:3804 -7.8 -19.9 60 -12.1 0 -3.8 TCTTCTGCAGCACGTTTTTC

547 SEQ ID N0:3805 -7.8 -24.7 73.3 -16.4 0 -8.3 TAAATAAATGGAAGGTTCTT

646 SEQ TD N0:3806 -7.8 -15.6 50.7 -7.8 0 -4.2 CTCCATAAATAAATGGAAGG

651 SEQ ID N0:3807 -7.8 -16.9 52.4 -6.8 -2.3 -7.8 GGAATGTCTCCATAAATAAA

658 SEQ ID N0:3808 -7.8 -17.3 53.7 -8.4 -1 -4 AGGAATGTCTCCATAAATAA

659 SEQ ID N0:3809 -7.8 -18 55.5 -8.4 -1.8 -4.8 CATCTCTGGAGGAATGTCTC

668 SEQ ID N0:3810 -7.8 -23.1 69.8 -14.6 -0.4 -6.1 CTTGATAGCAATTTTCCTAA

821 SEQ ID N0:3811 -7.8 -19.7 59.7 -11.3 -0.3 -0.5 AGATTTTTATAAGTGACTCA

972 SEQ ID N0:3812 -7.8 -17.7 56.9 -9.9 0 -4.7 GGTTTCGACCAGCCTTCACA

1527 SEQ ID N0:3813 -7.8 -28.1 77.5 -18.4 -1.9 -7.2 TAGCCATAGTTGGGGTTTCG

1540 5EQ ID N0:3814 -7.8 -25.4 73.2 -16.1 -1.4 -5.1 AAAAAATATCATGTATGTTT

1664 SEQ ID N0:3815 -7.8. -13.6 46.7 -5.3 -0.1 -5.8 ATTCACCAAATAAAATGAGC

1703 SEQ ID N0:3816 -7.8 -16.7 52.1 -8.9 0 -3 TCCTTTGTTGTTTCCTTCAA

2006 5EQ ID N0:3817 -7.8 -24 71.1 -16.2 0 -2.9 GAAAACAGGGAGCCACGGAT

2155 SEQ ID N0:3818 -7.8 -23.7 65.1 -15.3 -0.3 -4.3 GCCCGACCTCTGAAACTGAA

2203 SEQ ID N0:3819 -7.8 -25.8 68.6 -18 0 -2.8 CAATCCACAGTGCTGTGCAT

2401 SEQ ID N0:3820 -7.8 -25.6 72.6 -15.9 -1.2 -11.9 CATGCATCACAGCAGTCCCA

2689 SEQ ID N0:3821 -7.8 -28 77.8 -18.9 -1.2 -7.5 ATTAAATTCACAAGATGTTT

271g SEQ ID N0:3822 -7.8 -15.8 51.6 -8 0 -3.2 TCCCTCCACATTTGACAGAC

2gg0 SEQ TD N0:3823 -7.8 -25.8 72.4 -18 0 -2.4 CCAGCGACCTCCATACAGTC

3319 SEQ ID N0:3824 -7.8 -28.3 77.1 -20.5 0 0 CAAGGTTTCCAATGACCATG

3372 SEQ ID N0:3825 -7.8 -22.5 64.3 -13.1 -1.5 -5.8 AGGTTCAGAACCACAAGGTT

3385 SEQ ID N0:3826 -7.8 -23.1 67.6 -12.6 -2.7 -12 kcal/

mot kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CGGTTTAAAGTCTTCTTCGG

3947 SEQ ID N0:3827 -7.8 -22.1 64.9 -13.6 -0.5 -4.7 TATATCTTCAAAGGCCAATG

4136 SEQ ID N0:3828 -7.8 -19.5 58.7 -11.2 0 -7.7 CAGGCTAACCAAAGAAACAT

4313 SEQ ID N0:3829 -7.8 -19.2 56.5 -10 -1.3 -3.7 GATTTGATGGCACCGAGTTC

4357 SEQ ID N0:3830 -7.8 -24.4 69.9 -16.6 0 -4 GAGGTCTTAAAGCTCTTAAT

4386 SEQ ID N0:3831 -7.8 -19.8 61.2 -11.5 -0.1 -5.9 GATAAGGCTCTTAGAGGTCT

4399 SEQ ID N0:3832 -7.8 -22.9 69.8 -14.5 -0.3 -6.1 CCGGGATAAGGCTCTTAGAG

4403 SEQ ID N0:3833 -7.8 -24.4 69.3 -16.1 -0.2 -6.1 TGTCAAACATGTTACCCGTT

4575 SEQ ID N0:3834 -7.8 -23.1 65.6 -14.7 0 -8.5 GTTTCTTCATTGCATTGTAA

4947 SEQ ID N0:3835 -7.8 -20.8 64.3 -13 0 -6.2 GAGGCAGATGAGGATCATGA

5066 SEQ ID N0:3836 -7.8 -23 68 -13.1 -2.1 -7.8 CCATCATGGTGACCATGTTG

5085 SEQ ID N0:3837 -7.8 -25 70.9 -14 -3.2 -11.5 GATGTTCCAGCCTATAGTGA

5225 SEQ ID N0:3838 -7.8 -24.9 72.8 -17.1 0 -5.1 GTGAATTGTGTCAGGGTCAC

5648 SEQ ID N0:3839 -7.8 -23.6 71.4 -15.2 -0.3 -5.2 GGGATCAAACTTTTCCCAAA

5858 SEQ ID N0:3840 -7.8 -21.8 62.2 -11.9 -2.1 -7.7 TAGAGAACTCTATAAACTGG

5886 SEQ ID N0:3841 -7.8 -16.9 53.9 -8.6 0 -7.5 AAGCTGGACTTTGTTGGGTT

5954 SEQ ID N0:3842 -7.8 -24.1 71.2 -16.3 0 -5.1 ATCCATCTCTCCACTCTCAC

6056 SEQ ID N0:3843 -7.8 -26.5 77.4 -18.7 0 -0.5 GAGACTTTGGAGGGGTTTGA

6112 SEQ ID N0:3844 -7.8 -23.9 70.9 -16.1 0 -2.6 TTGCTTTAAAAGATAACATC

6206 SEQ ID N0:3845 -7.8 -15.5 50.7 -7.7 0 -4.3 GTAAGTCAATCCTCCCTTTA

6267 SEQ ID N0:3846 -7.8 -24.3 70.3 -16.5 0 -2.4 GTTTGTCTTTCTCAAACTTT

6408 SEQ ID N0:3847 -7.8 -20.4 63.7 -10.2 -2.4 -7.4 AGATAATTCTTTGTTTCTTT

6475 SEQ ID N0:3848 -7.8 -18.1 58.5 -10.3 0 -2.9 ACTATGAGTATTTGTTAAAA

6578 SEQ ID N0:3849 -7.8 -15.4 51 -7.6 0 -2.8 AGGTCACTTCACTGTCTTGT

6608 SEQ ID N0:3850 -7.8 -24.7 75.7 -16.9 0 -2.6 AATAGTGACATAGCATTATG

6885 SEQ ID N0:3851 -7.8 -17.7 56 -8.8 -1 -4.7 AGTGCAAAAGTAAAGGTGTT

7702 SEQ ID N0:3852 -7.8 -18.8 57.9 -11 0 -5.4 GATACTCATGTTGAATTAAA

7722 SEQ ID N0:3853 -7.8 -16.1 52.1 -8.3 0 -4.7 AGTTTTAGACATATTTTTAG

7816 SEQ ID N0:3854 -7.8 -16.5 54.7 -8 -0.4 -2.9 AAAACCCATTATCTAACTTT

8021 SEQ ID N0:3855 -7.8 -18.2 55.2 -10.4 0 -1.9 kcal) mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TAATTATATATATTTTAAAC

8536 SEQ ID N0:3856 -7.8 -10.7 41.2 -2.9 0 -5 ACATGTGAACTTGATCCTTT

8693 SEQ ID N0:3857 -7.8 -21.3 63.5 -13.5 0 -6.1 TATTGTAGGTGGTTACTACT

8738 SEQ ID N0:3858 -7.8 -21.2 65.9 -11.8 -1.6 -5.2 TGAGTTTAGATGCTTATAGC

8784 SEQ ID N0:3859 -7.8 -20.3 63.5 -11.6 -0.8 -4.2 ACCTTATGTATACATAGGAG

8892 SEQ ID N0:3860 -7.8 -19.9 61.1 -9.9 -1.1 -12.6 TGCTCCTTTCCCAGTAAGCC

308 SEQ ID N0:3861 -7.7 -29.3 80.6 -20.7 -0.8 -4.5 GGAAGCTTTCAGGTCCTGGG

495 SEQ ID N0:3862 -7.7 -27.2 77.9 -18 -0.8 -10.8 TACCAAAATCTTGATAGCAA

830 SEQ ID N0:3863 -7.7 -18 55 -9.7 -0.3 -4.5 GTCAAAATAGTGCACATGAT

871 SEQ ID N0:3864 -7.7 -19.1 58.3 -10.4 0 -9.9 ACATATGCCATCACAATGAC

1060 SEQ ID N0:3865 -7.7 -21.2 62 -12.8 -0.5 -8.3 ACTCTGAATGTTCTCAACGC

1111 SEQ ID N0:3866 -7.7 -22.1 65.1 -13.5 -0.8 -4.6 GCCCCCACAATGGTCTTTAA

1168 SEQ ID N0:3867 -7.7 -27.6 73.9 -19 -0.7 -4.3 CCCATGAACAGCTGCAGCCC

1264 SEQ ID N0:3868 -7.7 -30.5 79.5 -20.3 0 -13.2 GCTTGGGGGCCACTGCAAAC

1301 SEQ ID N0:3869 -7.7 -28.5 76.9 -19.3 -l.4 -7.1 CATTTCCACAGAGTAAAGGG

1470 SEQ ID N0:3870 -7.7 -21.4 63.3 -13.7 0 -3.5 CCTTTGTTGTTTCCTTCAAG

2005 SEQ ID N0:3871 -7.7 -23.6 69.7 -15.9 0 -3.1 TGCTTCTTTTGACGCTGTCT

2058 SEQ ID N0:3872 -7.7 -25.2 73.6 -15.5 -2 -7.6 GCTCTCCATGTCTGTGCGGC

2310 SEQ ID N0:3873 -7.7 -30.4 85.5 -22.7 0 -5.1 TGGTGCCCTCTGGGGGAAGT

2478 SEQ ID N0:3874 -7.7 -30.3 83.3 -21 -1.5 -8 AGAGGGTATTTAAGACAATG

2778 5EQ TD N0:3875 -7.7 -17.9 56.3 -10.2 0 -2.6 TCTGCTGTGAAAATCCCAGT

2869 SEQ ID N0:3876 -7.7 -24 68.4 -15.8 -0.1 -3.6 GGCTGACAATAATTCCATCA

2952 SEQ ID N0:3877 -7.7 -22 63.9 -14.3 0 -3.7 CTCTAAGCAGTCTGAATGAT

3024 SEQ ID N0:3878 -7.7 -20.7 63 -13 0 -6.3 CGGGTTCAGTTTCAGCTTGT

3930 SEQ ID N0:3879 -7.7 -26.1 76.9 -18.4 0 -4.5 TCAGTAAAACAAGCTTCCGG

3964 SEQ ID N0:3880 -7.7 -21.3 61.5 -13.6 0 -6.4 GAAGCATTTCCAGAATGAAT

4221 SEQ ID N0:3881 -7.7 -19.5 58.4 -10.2 -1.6 -5.7 GAAATATGTTTGAAATCCAT

4256 SEQ ID N0:3882 -7.7 -16.6 52.3 -8.9 0 -4.1 TGAAATATGTTTGAAATCCA

4257 SEQ ID N0:3883 -7.7 -16.6 52.3 -8.9 0 -4.1 AGGTCTTAAAGCTCTTAATG

4385 SEQ ID N0:3884 -7.7 -19.2 59.8 -11.5 0 -5.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CAGCAAACAAATTCACACCC

4527 SEQ ID N0:3885 -7.7 -21.6 61 -13.9 0 -4.1 CCAGCAAACAAATTCACACC

4528 SEQ ID N0:3886 -7.7 -21.6 61 -13.9 0 -4.1 TAATGTCAAACATGTTACCC

4578 SEQ ID N0:3887 -7.7 -20 59.4 -11.1 -1.1 -9 GGCAGATGAGGATCATGATG

5064 SEQ ID N0:3888 -7.7 -22.4 66.3 -13.1 -l.6 -8.5 GTTGAGGCAGATGAGGATCA

5069 SEQ ID N0:3889 -7.7 -23.7 70.5 -14.8 -1.1 -6 GTCATCCGTTTCCACCATCA

5099 SEQ ID N0:3890 -7.7 -28 77.5 -20.3 0 -2.6 AAAACTAGGGTCATGTATTT

5125 SEQ ID N0:3891 -7.7 -18.5 57.6 -10.8 0 -4.7 CAAATTCTCCAGTGAACAGA

5172 SEQ ID N0:3892 -7.7 -20.4 60.7 -12.7 0 -3.9 TTCTATCATCTCAGCCAGAA

5285 SEQ ID N0:3893 -7.7 -23 68.6 -15.3 0 -3.6 TTCCCAAACCTCATAGAACA

5846 SEQ TD N0:3894 -7.7 -22.5 63.5 -14.8 0 -2.7 ACATCTGAAATTACGCTGAA

6191 SEQ ID N0:3895 -7.7 -18.8 56.4 -11.1 0 -3.3 AACATCTGAAATTACGCTGA

6192 SEQ ID N0:3896 -7.7 -18.8 56.4 -11.1 0 -3.3 GGGTGGTAGAGGAACTCCCA

6348 SEQ ID N0:3897 -7.7 -27.6 77.5 -17.2 -2.7 -8.2 GTAGCCATTGGGTTTCTCCT

6707 SEQ ID N0:3898 -7.7 -27.9 80.6 -19.3 -0.8 -6.3 TCACTTTGCACAACTATCCC

6737 SEQ ID N0:3899 -7.7 -24.3 69.2 -16.6 0 -5 TTATGAATAAACTATGAATT

6870 SEQ ID N0:3900 -7.7 -12.8 45.1 -5.1 0 -3.1 6896 SEQ ID N0:3901 -7.7 -14.8 49.3 -6.4 -0.5 -5 CCATCTAGAAAATGGAAGTA

7042 SEQ ID N0:3902 -7.7 -18.2 55.7 -9.5 -0.9 -7.2 AAAATTAAAGCCATCTAGAA

7052 SEQ ID N0:3903 -7.7 -15.7 50.2 -8 0 -6.2 TCTAGAATGGATTTATTAGA

7249 SEQ ID N0:3904 -7.7 -17 55 -9.3 0 -5.2 TGAAGAATGGATTGAGTAAA

7587 SEQ ID N0:3905 -7.7 -15.8 51 -8.l 0 -2.1 TATGATACTCATGTTGAATT

7725 SEQ ID N0:3906 -7.7 -17.5 55.7 -8.9 -0.8 -4.9 ATATGATACTCATGTTGAAT

7726 SEQ ID N0:3907 -7.7 -17.4 55.4 -8.8 -0.8 -5.1 AGACATATTTTTAGCAAAAT

7810 SEQ ID N0:3908 -7.7 -15.7 51.1 -6.8 -1.1 -5.3 GGAAATTATCCATTGAAAAA

7994 SEQ ID N0:3909 -7.7 -15.2 49 -6 -1.4 -6.8 GTAATAGCTTTAGTGCAGCA

8227 SEQ ID N0:3910 -7.7 -22.7 68.6 -13.4 -1.5 -6.5 AGTTCTTCAATACACTACAG

8381 SEQ ID N0:3911 -7.7 -19.4 60.7 -11.7 0 -3.1 AGCAATTCCCTGCATGCAGT

8398 SEQ ID N0:3912 -7.7 -27.4 76.3 -16.6 -1.9 -14.3 TATTCTTTTGGCTCAATAAT

8446 SEQ ID N0:3913 -7.7 -18.7 58.3 -11 0 -4.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATCAAACCTGAAAGTTGGAA

8712 SEQ TD N0:3914 -7.7 -18.4 55.6 -10 -0.4 -3.8 TTTGGTTTTGACCTCACATA

8970 SEQ ID N0:3915 -7.7 -22.3 66.5 -12.3 -2.3 -4.8 CCAAGATGGTTAGGGTATAA

158 SEQ ID N0:3916 -7.6 -21.1 62.7 -13 -0.2 -3.7 AGTTTTCTTGCTCCATAATC

226 SEQ ID N0:3917 -7.6 -22.1 67.2 -14.5 0 -4.4 CTTCTGTGAATTATCTCATT

359 SEQ ID N0:3918 -7.6 -19.7 61.6 -12.1 0.4 -3.2 TTGCATACGAATTACCTTAG

417 SEQ ID N0:3919 -7.6 -19.9 59.2 -12.3 0 -5.1 TTCTTTCCAGCTTCCAAGTC

631 SEQ ID N0:3920 -7.6 -25.5 75.1 -17.9 0 -4.5 CCATGAACAGCTGCAGCCCA

1263 5EQ ID N0:3921 -7.6 -29.2 77.2 -19.1 0 -13.2 ACAAATGTCCCATTTGAATC

1369 SEQ ID N0:3922 -7.6 -20.3 59.9 -8.9 -3.8 -10.4 GTCAACTGGTAAAGATTTTC

1627 SEQ ID N0:3923 -7.6 -18.7 58.6 -11.1 0 -3.2 ACAAAAAATATCATGTATGT

1666 SEQ ID N0:3924 -7.6 -14.3 47.9 -6.7 0 -4.7 GGAGCAGAATTTTTTGTCAC

2114 SEQ ID N0:3925 -7.6 -21.2 64.6 -13.6 0 -4.l TTCATCATCAGCAAAGTCAT

2243 SEQ ID N0:3926 -7.6 -20.8 63.4 -13.2 0 -4.1 ATACTGGCCTGACTAACGTT

2341 SEQ ID N0:3927 -7.6 -23.7 67.2 -15.6 0 -7.8 TTGTCCAGTAGGTGACGTTA

2459 SEQ ID N0:3928 -7.6 -24.3 71.8 -15.6 -1 -7 AAGCTCCATTAAACTGAGGC

2969 SEQ TD N0:3929 -7.6 -22.2 64.5 -14.1 -0.1 -5 ATTGCCAATGATCTTAATTA

3083 SEQ ID N0:3930 -7.6 -18.7 57.3 -11.1 0 -4.9 CACAGAATTGCCAATGATCT

3089 SEQ ID N0:3931 -7.6 -21.3 62 -13.7 0 -4.9 CAGGCATTAGTGAAATATGT

4267 SEQ ID N0:3932 -7.6 -19.5 59.6 -11.9 0 -4 TGCCAGCAAACAAATTCACA

4530 SEQ ID N0:3933 -7.6 -21.2 60.7 -13.6 0 -5.2 TTGCATTGTAATATTTTTTC

4938 SEQ ID N0:3934 -7.6 -17.5 56.4 -9.9 0 -5.1 ATGATGCTGATATCAAAGAC

5050 SEQ ID N0:3935 -7.6 -18.1 56.3 -9.6 -0.8 -7.7 AGATGTTCCAGCCTATAGTG

5226 SEQ ID N0:3936 -7.6 -24.3 71.7 -16.7 0 -5.1 AGGATTCGGCCAATCCTGGC

5347 SEQ ID N0:3937 -7.6 -28.4 76.7 -17.1 -3.3 -15.1 TCTCAAAGTTGAACATGTCA

5532 SEQ ID N0:3938 -7.6 -19.4 59.8 -11.3 -0.1 -7 CAGGGTGAATTGTGTCAGGG

5652 SEQ ID N0:3939 -7.6 -24.2 71.3 -16.6 0 -3.3 TCTTCAGTAGCAACACTGAA

5791 SEQ ID N0:3940 -7.6 -21.1 63.7 -10.6 -2.9 -9.6 CATCTCAAAGTCATCCTCAC

5828 SEQ ID N0:3941 -7.6 -22.6 67.2 -15 0 -1.9 TCCCAAACCTCATAGAACAT

5845 SEQ ID N0:3942 -7.6 -22.4 63.2 -14.8 0 -2.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GAACTCTATAAACTGGGTCG

5882 SEQ TD N0:3943 -7.6 -20.2 60.1 -12.6 0 -2.7 TGGGTTTTGCTATGAGAAGA

5940 SEQ ID N0:3944 -7.6 -21.4 64.5 -13.8 0 -3.6 AGAGGAACTCCCATCTGTTT

6341 SEQ ID N0:3945 -7.6 -24.6 71.2 -14.6 -2.4 -6.7 GTTTCTCCTCAGCAGTGTCA

6696 SEQ ID N0:3946 -7.6 -27.1 81.9 -19.5 0 -4.2 TTCACTTTGCACAACTATCC

6738 SEQ ID N0:3947 -7.6 -22.4 66 -14.8 0 -5 TGGGGACCATGGAAATGCAC

6851 SEQ ID N0:3948 -7.6 -24.2 67.2 -14.4 -2.2 -9.8 TATGAATAAACTATGAATTG

6869 SEQ ID N0:3949 -7.6 -12.7 44.8 -5.1 0 -3.1 ATTATGAATAAACTATGAAT

6871 SEQ ID N0:3950 -7.6 -12.7 44.8 -5.1 0 -3.5 GTTCAGATAGAAACAAACAT

7091 SEQ ID N0:3951 -7.6 -16.3 52.1 -8 -0.5 -3.7 TATATATATATTTGCCCAAA

7418 SEQ ID N0:3952 -7.6 -17.8 54.8 -10.2 0 -6.7 TTATATTTAAACAAGTTTTA

7829 SEQ ID N0:3953 -7.6 -13.4 46.8 -5.8 0 -5.5 CACAAAGCTGCTTTTAATTT

8658 5EQ TD N0:3954 -7.6 -19.1 58.1 -10 -0.3 -10.9 TTTATATAATGCATTTAAGT

8872 SEQ ID N0:3955 -7.6 -15.5 51.3 -7.2 0 -8.8 GAGTTTGGTTTTGACCTCAC

8973 SEQ TD N0:3956 -7.6 -23.7 70.9 -13.8 -2.3 -6.2 TAAGAGTTTGGTTTTGACCT

8976 SEQ TD N0:3957 -7.6 -21.4 64.8 -11.5 -2.3 -6.3 TTCTCTGTGGATAAGAAAAC

47 SEQ ID N0:3958 -7.5 -17.4 55 -9.9 0 -3.5 TCTAGTAAAAAGGCGGAAGC

509 SEQ TD N0:3959 -7.5 -19.8 58.7 -10.7 -1.6 -7.2 CGTTTTTCGATAGCAGCAAG

535 SEQ ID N0:3960 -7.5 -22.2 64.4 -14.7 0 -5.4 CAGCTTCCAAGTCACTATTT

624 SEQ TD N0:3961 -7.5 -23.2 68.7 -15.7 0 -4.5 AAATAAATGGAAGGTTCTTT

645 SEQ TD N0:3962 -7.5 -16 51.6 -8.5 0 -4.2 AAGGTCATAAATACACAGTT

892 SEQ ID N0:3963 -7.5 -17.9 56.1 -10.4 0 -2.8 TCCAGTCAGGAGGGTTGCTC

912 SEQ ID N0:3964 -7.5 -28 82.7 -19.5 -0.9 -6.2 GAAATCCAGCCAGTTCCATG

1037 SEQ ID N0:3965 -7.5 -25.2 70.3 -17.7 0 -3.9 GGGCCCCCACAATGGTCTTT

1170 SEQ ID N0:3966 -7.5 -31 81.6 -21.7 -0.7 -11.8 ACGGATACTCAAGAGAGACT

2141 SEQ ID N0:3967 -7.5 -20.9 62.2 -11.8 -1.5 -6.2 GACATACTGGCCTGACTAAC

2344 SEQ ID N0:3968 -7.5 -23.1 66.5 -15.6 0 -7.2 GCTGGAAGCCCTGGCACCAT

2371 SEQ ID N0:3969 -7.5 -31.1 81.6 -22 -1.5 -10.6 TCCACAGTGCTGTGCATCTT

2398 SEQ ID N0:3970 -7.5 -27 78.3 -17.3 -1.7 -12.4 TGTCCAGTAGGTGACGTTAG

2458 SEQ ID N0:3971 -7.5 -24.2 71.7 -15.6 -1 -7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GAGGAATCCTCCAGCATCTC

2551 SEQ ID N0:3972 -7.5 -26.3 75.4 -16.9 -1.9 -10 ACAAGATGTTTTACTTTTAA

2710 5EQ ID N0:3973 -7.5 -16.4 53.3 -8.9 0 -2.9 TCCATCAAAGATATTCCAGC

2939 SEQ ID N0:3974 -7.5 -22.1 64.6 -13.9 -0.4 -3.5 AACAATAAGGCACATGGTTT

3341 SEQ TD N0:3975 -7.5 -19.7 59 -12.2 0 -5.2 AATCAATTCCCTTTTGCATT

3501 SEQ ID N0:3976 -7.5 -22 64 -14.5 0 -5.1 TCATTTTCATCGATTACGTA

3718 SEQ ID N0:3977 -7.5 -20 60.5 -11.6 -0.7 -6.3 TATAATCATTTTCATCGATT

3723 SEQ TD N0:3978 -7.5 -17.1 54.5 -9.6 0 -6.3 CTGTGCTTCCTTCAGATGAG

3882 SEQ ID N0:3979 -7.5 -24.6 72.7 -17.1 0 -3.7 GTTCAGTTTCAGCTTGTTCA

3927 SEQ ID N0:3980 -7.5 -24.1 74.9 -16.6 0 -4.5 GTAGCAGGTTTTTCGAAGAT

4052 SEQ ID N0:3981 -7.5 -21.9 65.8 -13.7 -0.4 -6.8 CCACTACTGAGAAGGATCAT

4114 SEQ ID N0:3982 -7.5 -22 64.5 -13.6 -0.8 -6.3 ATCTTCAAAGGCCAATGCAC

4133 SEQ ID N0:3983 -7.5 -22.8 65.4 -13.8 -1.4 -7.7 AATGATTTGATGGCACCGAG

4360 SEQ ID NO:3984 -7.5 -22 62.8 -14.5 0 -4 TAGAGGTCTTAAAGCTCTTA

4388 SEQ ID N0:3985 -7.5 -20.2 63 -11.5 -1.1 -5.2 TTGCTCCAACAAGAGCATTC

4446 SEQ ID N0:3986 -7.5 -23 66.9 -12.1 -3.4 -9.5 TTGCCAGCAAACAAATTCAC

4531 SEQ ID N0:3987 -7.5 -20.6 59.9 -13.1 0 -7.1 TGCTGGTTGAAGTTATCTAT

4873 SEQ ID N0:3988 -7.5 -21.1 64.8 -13.6 0 -3.6 ATATTTTTTCTGTTCCTCTG

4928 SEQ ID N0:3989 -7.5 -21.4 66.3 -13.9 0 -1.8 CATTGTAATATTTTTTCTGT

4935 SEQ TD N0:3990 -7.5 -17.7 56.9 -10.2 0 -4.2 TGCATTGTAATATTTTTTCT

4937 SEQ ID NO:3991 -7.5 -18.3 58 -10.8 0 -4.7 TCATTGCATTGTAATATTTT

4941 SEQ ID N0:3992 -7.5 -18 57 -10.5 0 -6.2 TTCATTGCATTGTAATATTT

4942 SEQ ID N0:3993 -7.5 -18 57 -10.5 0 -6.2 TTCTTCATTGCATTGTAATA

4945 SEQ TD N0:3994 -7.5 -19.2 60 -11.7 0 -5.6 TTGGATCCAAGTTTCTTCAT

4957 SEQ ID N0:3995 -7.5 -22.2 66.7 -13 0 -11.5 CATGATGCTGATATCAAAGA

5051 SEQ ID N0:3996 -7.5 -18.6 57 -10.2 -0.8 -7.7 TTGAGGCAGATGAGGATCAT

5068 SEQ ID N0:3997 -7.5 -22.5 67 -13.1 -1.9 -7.8 GCCCTGGTCATCCGTTTCCA

5105 SEQ ID N0:3998 -7.5 -31.9 85.1 -23.7 -0.4 -4.2 CACAAATTCTCCAGTGAACA

5174 SEQ ID N0:3999 -7.5 -20.7 61 -13.2 0 -4.2 TTTGCTTTAAAAGATAACAT

6207 SEQ ID N0:4000 -7.5 -15.2 4g,g -7.7 0 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATAGTTACTTGATATATTTT

6233 SEQ ID N0:4001 -7.5 -16.2 53.7 -8.7 0 -4.3 TCTTTTCCTTTGCTTTCTTT

6433 5EQ ID N0:4002 -7.5 -23.5 71.5 -16 0 -3.6 GGGTTTCTCCTCAGCAGTGT

6698 SEQ ID N0:4003 -7.5 -28.4 84.6 -20 -0.8 -5.7 GTACTAAAGGTGTTTGGTGT

6768 SEQ ID N0:4004 -7.5 -21.7 66.5 -13.3 -0.8 -4.6 CTTTTTCTGGTTTTGAGCAA

6998 SEQ ID N0:4005 -7.5 -21.7 65.9 -14.2 0 -4.1 GTGAAGAATGGATTGAGTAA

7588 SEQ ID N0:4006 -7.5 -17.7 55.6 -10.2 0 -2.1 TGAATTCATTCTATCACCAA

7634 SEQ ID N0:4007 -7.5 -19.5 59.2 -11.3 -0.4 -8 CAAAATATGAAGAATAGGTT

7796 SEQ ID N0:4008 -7.5 -14.3 47.8 -6.8 0 -2.7 TTCATGGCTAAAAAGAACAA

8263 SEQ ID N0:4009 -7.5 -16.1 51 -8.6 0 -4.7 ATTAACATTAAACTTTTGGT

8341 SEQ TD N0:4010 -7.5 -16.2 52.3 -8.7 0 -3.5 TGCAATAAAAAATGAAATTT

8465 SEQ ID N0:4011 -7.5 -11.4 41.9 -2.8 -1 -6.7 AAATATGCATTACAACTGGA

9011 SEQ ID N0:4012 -7.5 -17.7 54.6 -10.2 0 -6.8 AGCATAACAAAGCCCAGCAT

178 SEQ ID N0:4013 -7.4 -24 66.5 -15.7 -0.7 -5 GAGATTCTTTGCTCCTTTCC

317 5EQ ID N0:4014 -7.4 -25.4 74.8 -18 0 -4.2 CCTTCTGTGAATTATCTCAT

360 SEQ ID N0:4015 -7.4 -21.6 65.1 -14.2 0.4 -3.2 CCGGAAAAGCTCCAGGTCCC

378 SEQ ID N0:4016 -7.4 -29.1 75.6 -20.1 -1.5 -5.6 TGGGCTTCTTGGCTTTCTCT

564 SEQ ID N0:4017 -7.4 -27.3 80.6 -18.9 -0.9 -3.7 CAAGTCACTATTTGGCTTTG

617 SEQ ID N0:4018 -7.4 -21.4 64.6 -12.4 -1.5 -5 CCATCTCTGGAGGAATGTCT

669 SEQ ID N0:4019 -7.4 -24.7 71.9 -16.5 -0.6 -6.l AGTTTTCTTATTGATATAGT

719 SEQ ID N0:4020 -7.4 -17.9 58.6 -10.5 0 -2.7 TGAATCGGAAAATTGCCTTT

759 SEQ ID N0:4021 -7.4 -19.8 57.7 -12.4 0 -4.4 AAAATATACAAGGCAGAGGT

784 SEQ ID N0:4022 -7.4 -18.2 55.9 -10.8 0 -4 AAAATCTTGATAGCAATTTT

826 SEQ ID N0:4023 -7.4 -15.7 51 -7.5 -0.6 -1.2 AGGGCCCCCACAATGGTCTT

1171 SEQ ID N0:4024 -7.4 -30.9 81.6 -21.7 -0.7 -11.8 TCTCCAATGTAATCCTTCCA

1414 SEQ ID N0:4025 -7.4 -24.4 69.5 -17 0 -2.2 ACCAGCCTTCACACAGATGT

1520 SEQ ID N0:4026 -7.4 -26.4 74.5 -18.4 -0.3 -3.8 TAGTTGGGGTTTCGACCAGC

1534 SEQ TD N0:4027 -7.4 -26.5 75.8 -16.4 -2.7 -7.1 CAACTTTGATGCTTCTGAAG

1925 SEQ ID N0:4028 -7.4 -19.9 60.3 -12 0 -7.8 AGCATCTCCATTGAAATCTG

2539 SEQ ID N0:4029 -7.4 -21.5 63.7 -14.1 0 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAAATGAAGACGATGATGGC

3139 SEQ ID N0:4030 -7.4 -17.6 53.5 -10.2 0 -3.5 AGAGCTGCATGCCGACCACA

3162 SEQ ID N0:4031 -7.4 -28.8 76.8 -20.4 -0.8 -9.l TGCAGATTATTCATTTCATT

3466 SEQ ID N0:4032 -7.4 -19.5 60.8 -11.6 -0.2 -4.9 TTTCATCGATTACGTATTTT

3714 SEQ ID N0:4033 -7.4 -19.1 58.6 -10.8 -0.7 -6.3 ACATATAATCATTTTCATCG

3726 SEQ ID N0:4034 -7.4 -17.3 54.6 -9.9 0 -2.5 GGTTCAGTTTCAGCTTGTTC

3928 SEQ ID N0:4035 -7.4 -24.6 76.7 -17.2 0 -4.5 TATCTTCAAAGGCCAATGCA

4134 SEQ ID N0:4036 -7.4 -22.3 64.3 -13.4 -1.4 -7.7 GTATATATCTTCAAAGGCCA

4139 SEQ ID N0:4037 -7.4 -21.1 63.2 -13.2 0 -7.7 TCTAGCCAGCACCAGGCATT

4279 SEQ ID N0:4038 -7.4 -28.7 79.8 -18.6 -2.7 -7.4 CAACAGCACATTCATGATAG

4472 SEQ ID N0:4039 -7.4 -19.8 59.8 -12.4 0 -6.4 TGGTTGAAGTTATCTATGAT

4870 SEQ ID N0:4040 -7.4 -19 59.8 -11.6 0 -2.6 CTATAGTGAAGTAGTAGTGT

5214 SEQ ID N0:4041 -7.4 -19.6 62.9 -12.2 0 -4.3 AGCCTATAGTGAAGTAGTAG

5217 SEQ ID N0:4042 -7.4 -21 65 -13.6 0 -5.1 TGTCATCAATTCCAGCTTCC

5517 SEQ ID N0:4043 -7.4 -25.1 72.9 -17.7 0 -4.5 TACATGTTCACCACAACCAG

5749 SEQ ID N0:4044 -7.4 -23 65.8 -14.9 -0.4 -6.5 TGACCGCGATGTACATGTTC

5760 SEQ ID N0:4045 -7.4 -24.3 68.3 -16.2 0 -8.9 GCAACACTGAAGTTCTCCAG

5782 SEQ ID N0:4046 -7.4 -23.4 68.1 -15.5 -0.2 -6.3 GGCAGATCCATGGCAATAAG

5971 SEQ ID N0:4047 -7.4 -23.4 66.7 -15.1 -0.7 -8 TGACCATGGGCAGATCCATG

5979 SEQ ID N0:4048 -7.4 -26.1 72.4 -15.8 -2.9 -8.8 TGGATCCGGTCACCACTGAC

5995 SEQ ID N0:4049 -7.4 -27.3 74.9 -16.5 -l.8 -14.9 TGAATGATAGCGGCAGACAC

6175 SEQ ID N0:4050 -7.4 -22.2 64 -14.8 0 -6.6 TAGTTACTTGATATATTTTT

6232 SEQ ID N0:4051 -7.4 -16.3 54.1 -8.9 0 -4.3 CATAGGAAGGAGGAGGGGTG

6363 SEQ ID N0:4052 -7.4 -23.9 69.7 -16.5 0 -1.2 CTTGTATAGGCACTGACTAT

6593 SEQ ID N0:4053 -7.4 -21.8 65.8 -13.7 -0.4 -4.8 GGTTTCTCCTCAGCAGTGTC

6697 SEQ ID N0:4054 -7.4 -27.6 83.8 -20.2 0 -4.2 CAATGTTCACTTTGCACAAC

6743 SEQ ID N0:4055 -7.4 -20.6 61.5 -13.2 0 -5 AAATAGTGACATAGCATTAT

6886 SEQ ID N0:4056 -7.4 -17 54.2 -9.6 0 -4.1 CTCATTTACAAAAATAGTGA

6897 SEQ ID N0:4057 -7.4 -15.5 50.6 -7.4 -0.5 -5 ATTTGATTTAATAGAAGTTG

7448 SEQ ID N0:4058 -7.4 -14.9 50.1 -7.5 0 -2.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AACTATGTTTAATGAATGAT

7521 SEQ ID N0:4059 -7.4 -15 49.7 -7.6 0 -3.1 TTTTTAGCAAAATATGAAGA

7803 SEQ ID N0:4060 -7.4 -14.7 48.9 -6.8 -0.1 -5.3 TTTTCTTATGTACAAAATGC

7882 SEQ ID N0:4061 -7.4 -16.9 54.1 -9.5 0 -6.8 TATCATGTTAGGTAATTGCA

8299 SEQ ID NO:4062 -7.4 -19.8 61.3 -12.4 0 -5.1 TAGCTCACCATTCTTTTTAG

8422 5EQ ID N0:4063 -7.4 -21.9 66.6 -14.5 0 -4.8 TCTTTTGGCTCAATAATGAC

8443 SEQ ID N0:4064 -7.4 -19.7 60.3 -11.6 -0.5 -5.3 ACAAAAAACCCCAGAGCCCA

8495 SEQ ID N0:4065 -7.4 -25.4 66 -18 0 -3.2 TTTTAAACTGCCAACAGCAA

8524 SEQ ID NO:4066 -7.4 -20.2 59 -11.2 -1.5 -8.9 CCTTTCCAGAATCCTCTCTG

20 SEQ ID N0:4067 -7.3 -26 73.8 -17.8 -0.7 -4.2 GTAAAAAGGCGGAAGCTTTC

505 SEQ ID N0:4068 -7.3 -20.3 59.7 -10.6 -2.4 -7.2 CTTTCCAGCTTCCAAGTCAC

629 SEQ TD NO:4069 -7.3 -25.9 74.7 -18.6 0 -4.5 CTTTATTCATTACTATAAAA

738 SEQ ID NO:4070 -7.3 -14.2 48.3 -6.9 0 -3.3 GTACCAAAATCTTGATAGCA

831 SEQ ID N0:4071 -7.3 -19.9 59.6 -12 -0.3 -4.4 GATCACGAAGAAACGTAAAA

1017 SEQ ID N0:4072 -7.3 -15.6 49.2 -7.4 -0.8 -6.1 CCCAATGAGAGCAAACACGC

1247 SEQ ID N0:4073 -7.3 -24 65 -16.7 0 -4.1 CATGAACAGCTGCAGCCCAA

1262 SEQ ID N0:4074 -7.3 -26.5 71.7 -16.7 0 -13.2 AAGCAGAATCGCTTGGGGGC

1311 SEQ ID N0:4075 -7.3 -26.3 72.8 -17.6 -1.3 -8.4 TAAAAGTGACTGTCATCTCC

1429 5EQ ID N0:4076 -7.3 -20.5 62.1 -12.5 -0.1 -8.8 CACACAGATGTATCCTTCTG

1511 SEQ ID N0:4077 -7.3 -22.7 67.3 -14.1 -1.2 -5 TCACACAGATGTATCCTTCT

1512 SEQ ID N0:4078 -7.3 -23.1 69.1 -15.1 -0.5 -3.7 TGCTTTTTAAGCTGTTCGAG

1816 SEQ ID N0:4079 -7.3 -21.9 65.5 -12 -2.6 -9.4 TTTGGAACTCAACTTTGATG

1934 SEQ ID N0:4080 -7.3 -18.7 57.6 -10.8 -0.3 -5 GAGCAGAATTTTTTGTCACT

2113 SEQ ID N0:4081 -7.3 -20.9 64 -13.6 0 -4.1 CGGATACTCAAGAGAGACTG

2140 SEQ ID N0:4082 -7.3 -20.7 61.5 -11.8 -1.5 -5.4 TATTGCGTCTTGGGGAAAAC

2169 SEQ ID N0:4083 -7.3 -21.3 62 -14 0 -5.2 AAAATGCTTGTTTTGCTATT

2185 SEQ ID N0:4084 -7.3 -18.7 57.7 -10.9 -0.2 -4.7 CACTGTGACGGTGAGGCTGG

3761 SEQ ID N0:4085 -7.3 -26.7 74.9 -18.2 -1.1 -6.5 CTCCAACAGCAATTGGCACT

3777 SEQ ID N0:4086 -7.3 -24.9 69.5 -16 -l.5 -8.1 TCTTCTTCGGGTTCAGTTTC

3937 SEQ ID N0:4087 -7.3 -24.6 75.2 -17.3 0 -3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Tntra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligO

TCAACAATACTGTAGCAGGT

4063 SEQ ID N0:4088 -7.3 -21.2 63.8 -13 -0.7 -5.3 ATATATAGGTAAAGACTTTG

4203 5EQ ID N0:4089 -7.3 -15.7 51.8 -7.9 -0.1 -4.9 ACCAAAGAAACATCAACGAT

4306 SEQ ID N0:4090 -7.3 -17.6 52.8 -10.3 0 -3.5 GTTCTGAGTAGCCAAGAGCA

4341 SEQ ID N0:4091 -7.3 -25 73.6 -16.2 -1.4 -4.7 AGAGGTCTTAAAGCTCTTAA

4387 SEQ TD N0:4092 -7.3 -19.8 61.4 -11.5 -0.9 -5.6 ACCACCCTCATGCCTTCAAA

4423 SEQ ID N0:4093 -7.3 -27.6 73.5 -20.3 0 -4.4 AAGTTTAACATCTCGTGAAT

4754 SEQ ID N0:4094 -7.3 -18.2 56.4 -10.9 0 -3.9 GGACATCATCAAAGCAAAGA

5405 SEQ ID N0:4095 -7.3 -19.2 57.7 -11.3 -0.3 -4.4 CAACACTGAAGTTCTCCAGG

5781 SEQ ID N0:4096 -7.3 -22.8 66.4 -15.5 0.3 -6.3 CAGAGAGTTTAGAGAACTCT

5895 SEQ ID N0:4097 -7.3 -19.9 62.3 -8.6 -4 -9.5 CGGTCACCACTGACCATGGG

5989 5EQ ID N0:4098 -7.3 -28.2 75.5 -16.5 -4.4 -9.6 TGCTTTAAAAGATAACATCT

6205 SEQ ID N0:4099 -7.3 -16.3 52.2 -8.2 -0.6 -6.5 TAATTGCCTCTTTGTTATAG

6249 SEQ ID N0:4100 -7.3 -20 61.6 -12.7 0 -2.9 TAAAAATAGAATGGATGCAA

6793 SEQ ID N0:4101 -7.3 -14.1 47 -6.8 0 -5.6 TCTTTTTCTGGTTTTGAGCA

6999 SEQ ID N0:4102 -7.3 -22.8 69.9 -15.5 0 -4.1 TAAAGCCATCTAGAAAATGG

7047 SEQ ID N0:4103 -7.3 -17.5 53.8 -9.5 -0.5 -7.1 TGGATTGAGTAAAATAGAGC

7580 SEQ ID N0:4104 -7.3 -17.4 55 -10.1 0 -2.8 TATGAATAAAAGGTTATATT

8141 SEQ ID N0:4105 -7.3 -13.2 46 -5.9 0.2 -3 ACACTACAGTAAATAGTTTT

8370 SEQ TD N0:4106 -7.3 -17.3 55.5 -9.5 -0.1 -3.3 AAAAACCCCAGAGCCCAATA

8492 SEQ ID N0:4107 -7.3 -24.2 64.1 -16.9 0 -3.2 TTACAGCATAACAAAGCCCA

182 SEQ ID N0:4108 -7.2 -22.2 62.8 -15 0.6 -3.9 CTTGACAGGTATCTCATCCC

273 SEQ ID N0:4109 -7.2 -25.4 73.8 -18.2 0 -2.9 AAGTTTTCTTATTGATATAG

720 SEQ ID N0:4110 -7.2 -16 53.3 -8.1 -0.5 -3.7 GGCAGAGGTGGCACTGAATC

773 SEQ ID N0:4111 -7.2 -25.8 74.4 -17.7 -0.8 -4.7 GACTCAAAGGTATAGATTCC

958 SEQ ID N0:4112 -7.2 -20.3 61.8 -13.1 0 -2.5 AAAGTGACTGTCATCTCCAA

1427 SEQ ID N0:4113 -7.2 -21.5 63.9 -13.6 -0.1 -8.8 CAAAAAATATCATGTATGTT

1665 SEQ ID N0:4114 -7.2 -14.2 47.7 -7 0 -4.7 CTTCTTTTGACGCTGTCTTC

2056 SEQ ID N0:4115 -7.2 -23.9 71.4 -15.8 -0.8 -7.6 AGGAAGCTGCTTCTTTTGAC

2065 SEQ ID N0:4116 -7.2 -23 68.8 -13.2 -1.8 -13.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTCCAGTAGGTGACGTTAGA

2457 5EQ ID N0:4117 -7.2 -24.8 73.3 -17 -0.3 -7 TGGCAAATCTATACCAGCAT

2655 SEQ ID N0:4118 -7.2 -22.4 64.6 -14.5 -0.5 -4.5 AACACATTGGCAAATCTATA

2662 SEQ ID N0:4119 -7.2 -18.2 55.8 -11 0 -4.4 TTCACAAGATGTTTTACTTT

2713 SEQ ID N0:4120 -7.2 -18.5 58.5 -11.3 0 -3 GAGGCTGACAATAATTCCAT

2954 SEQ ID N0:4121 -7.2 -21.5 62.8 -14.3 0 -3.7 TGAGGCTGACAATAATTCCA

2955 SEQ ID N0:4122 -7.2 -21.5 62.8 -14.3 0 -3.7 AGCTCCATTAAACTGAGGCT

2968 SEQ ID N0:4123 -7.2 -23.8 68.5 -16.1 -O.l -4.6 ACAGAATTGCCAATGATCTT

3088 SEQ ID N0:4124 -7.2 -20.7 61.2 -13.5 0 -4.9 AGAAGTCGTTCATGTGCCAC

3240 SEQ ID N0:4125 -7.2 -24.6 71.3 -16.1 -1.2 -4.8 GAACCACAAGGTTTCCAATG

3378 SEQ ID N0:4126 -7.2 -22 62.9 -12.2 -2.6 -7.6 TGTCTATCTTATTGCCTTCA

3591 SEQ ID N0:4127 -7.2 -23.3 70.3 -16.1 0 -3 GATAATTAAGCTCTTTGCTT

3642 SEQ ID N0:4128 -7.2 -19.7 60.7 -10.3 -2.2 -8 AGATAATTAAGCTCTTTGCT

3643 SEQ ID N0:4129 -7.2 -19.6 60.5 -10.9 -1.4 -8 ATCGATTACGTATTTTTCAA

3710 SEQ ID N0:4130 -7.2 -18.3 56.4 -10.2 -0.7 -6.l GACATATAATCATTTTCATC

3727 SEQ ID N0:4131 -7.2 -17.1 55.1 -9.9 0 -2.5 AATTGGCACTGTGACGGTGA

3767 SEQ ID N0:4132 -7.2 -24 68.2 -15 -l.8 -7.6 GCTTTCTTCTAGTTCTGACT

3839 SEQ ID N0:4133 -7.2 -23.6 73.1 -15.9 -0.2 -4 TCAGATGAGCTGGTTGCATT

3871 SEQ ID N0:4134 -7.2 -24.1 71.5 -15.3 -1.5 -8 TTCAGATGAGCTGGTTGCAT

3872 SEQ ID N0:4135 -7.2 -24.1 71.5 -15.3 -1.5 -8 GTAAAACAAGCTTCCGGTTT

3961 SEQ ID N0:4136 -7.2 -21.6 62.3 -13.6 -0.5 -8.6 TGTATATATCTTCAAAGGCC

4140 SEQ ID N0:4137 -7.2 -20.4 61.9 -13.2 0 -6.4 TCAAGAAATCTAGCCAGCAC

4287 SEQ ID N0:4138 -7.2 -21.8 63.8 -14.6 0 -4.6 TAATGATTTGATGGCACCGA

4361 SEQ ID N0:4139 -7.2 -21.7 62.1 -14.5 0 -4 CAGTCACTCAAATTGTTAAC

4603 SEQ ID N0:4140 -7.2 -18.4 57,5 -11.2 0 -5.8 CATTGCATTGTAATATTTTT

4940 SEQ ID N0:4141 -7.2 -17.7 56 -10.5 0 -5.6 AGGCAGATGAGGATCATGAT

5065 SEQ ID N0:4142 -7.2 -22.4 66.7 -13.1 -2.1 -8.5 TGAGGCAGATGAGGATCATG

5067 SEQ ID N0:4143 -7.2 -22.4 66.6 -13.1 -2.1 -7.8 GAGCTTCAGCACAAATTCTC

5183 SEQ ID N0:4144 -7.2 -22.3 66.7 -14.2 -0.7 -6.6 GGATTCGGCCAATCCTGGCA

5346 SEQ ID N0:4145 -7.2 -29.1 77.4 -17.6 -4.3 -13.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CTCCTTTGATCAGACGTAGG

5364 SEQ ID N0:4146 -7.2 -23.8 69.3 -16.6 0 -6.5 TTCCTTTTTAACATAGGCAA

5501 SEQ TD N0:4147 -7.2 -20.4 61.2 -13.2 0 -4 CATGTTCACCACAACCAGGA

5747 SEQ ID N0:4148 -7.2 -24.9 69.5 -17 -0.4 -5.7 ACGCTTTGTAAAGGCAAATA

6029 SEQ ID N0:4149 -7.2 -19.4 57.6 -10.2 -2 -7.2 AGAGACTTTGGAGGGGTTTG

6113 SEQ ID N0:4150 -7.2 -23.3 69.8 -16.1 0 -2.6 TAGAGGAACTCCCATCTGTT

6342 SEQ ID N0:4151 -7.2 -24.2 70.2 -14.6 -2.4 -6.7 TTGATCACAAAGATAATTCT

6485 SEQ ID N0:4152 -7.2 -16.3 52.5 -8.4 -0.5 -6.5 TGTCAACGTTGATACCCTGC

6651 SEQ ID N0:4153 -7.2 -25.1 70.1 -15.6 -0.1 -12.8 CTCCTCAGCAGTGTCAGCTG

6692 SEQ ID N0:4154 -7.2 -28 82.2 -19 -l.8 -8 GTAACTGAAACTGTAGCCAT

7025 SEQ ID N0:4155 -7.2 -21 62 -13.8 0 -3.3 ATCTAGAAAATGGAAGTAAC

7040 SEQ ID N0:4156 -7.2 -15 49.6 -7.8 0 -6.2 ACATAACTGTTCAGATAGAA

7099 SEQ ID N0:4157 -7.2 -17.6 55.7 -9.8 -0.3 -5.6 ATTTGACCTAAATTAAATAT

7213 SEQ TD N0:4158 -7.2 -14.3 47.8 -6.6 -O.l -3.2 ACTATTTACATAACATAGGG

7377 SEQ ID N0:4159 -7.2 -17.6 55.5 -9.9 -0.1 -4.2 GTGCAAAAGTAAAGGTGTTT

7701 SEQ ID N0:4160 -7.2 -18.9 58.1 -11.7 0 -5.4 TAATTTTCTTATGTACAAAA

7885 SEQ ID N0:4161 -7.2 -14.1 48 -6.9 0 -6.8 AGTGTTTGACCAATGTATCT

8084 SEQ ID N0:4162 -7.2 -21.6 65.3 -14.4 0 -3.1 TTAGGTAATTGCACAACTTT

8292 SEQ ID N0:4163 -7.2 -19.3 59.2 -12.1 0 -5.1 GCTATTGTAGGTGGTTACTA

8740 5EQ ID N0:4164 -7.2 -22.8 69.9 -15.1 -0.1 -4 TTGAAAGCTATTGTAGGTGG

8746 SEQ ID N0:4165 -7.2 -20.3 62 -13.1 0 -4.6 TTATATAATTTATTATGGGA

9070 SEQ ID N0:4166 -7.2 -15.2 50.7 -7.2 -0.6 -5 TTTTTTTTTGACCACAGAGG

99 SEQ ID N0:4167 -7.1 -21 63.4 -13.4 -0.2 -3.6 TTTTTCCGGAAAAGCTCCAG

383 SEQ ID N0:4168 -7.1 -23.1 65 -12.9 -1.5 -14.4 GCAAACACGCTCAGACAGAA

1237 SEQ ID N0:4169 -7.1 -22.2 63 -15.1 0 -3.6 CTCCTCATAGGCCATGGCCA

1739 SEQ ID N0:4170 -7.1 -30.4 82.2 -18.7 -2.5 -17.4 TTCTGCTCCTCATAGGCCAT

1744 SEQ ID N0:4171 -7.1 -27.9 79.2 -20.1 -0.5 -7.7 CTTCTGAAGAACTTTCCAAC

1914 SEQ ID N0:4172 -7.1 -20 60.1 -12.9 0.3 -7.2 CATCCATGGAGAAAAGGAAG

2079 SEQ ID N0:4173 -7.1 -19.2 57.1 -10.8 -0.4 -10.4 GGGAGCAGAATTTTTTGTCA

2115 5EQ ID N0:4174 -7.1 -22.2 66.7 -15.1 0 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTTTACTTTTAACCATGCAT

2702 SEQ ID N0:4175 -7.1 -19.8 59.9 -12.7 0 -6.4 CCCCACAGAATTGCCAATGA

3092 SEQ ID N0:4176 -7.1 -26 69.1 -18.9 0 -4.3 CACATAATCAATTCCCTTTT

3506 SEQ ID N0:4177 -7.1 -20.7 61 -13.6 0 -2.5 TTCATCGATTACGTATTTTT

3713 SEQ ID N0:4178 -7.1 -19.1 58.6 -11.5 -0.1 -6.3 TTGTTTATGAATGACATATA

3739 SEQ ID N0:4179 -7.1 -16 52.3 -8 -0.7 -6 TCGGGGTAGAACAACATCAA

3902 SEQ ID N0:4180 -7.1 -21.1 61.3 -14 0 -3 AAAGTCTTCTTCGGGTTCAG

3941 SEQ ID N0:4181 -7.1 -22.6 67.9 -14.8 -0.5 -3.8 AGGCTAACCAAAGAAACATC

4312 SEQ ID N0:4182 -7.1 -18.9 56.5 -10.4 -1.3 -3.7 AGGCTCTTAGAGGTCTTAAA

4395 SEQ ID N0:4183 -7.1 -21.7 66.3 -14 -0.3 -5.3 GAGCATTCACAACCACCCTC

4434 SEQ ID N0:4184 -7.1 -26.9 73.8 -19.8 0 -4.1 GAATTGCTCCAACAAGAGCA

4449 SEQ ID N0:4185 -7.1 -22.4 64.3 -12.1 -3.2 -9.2 ATGATAGAGGGAATTGCTCC

4459 SEQ ID N0:4186 -7.1 -22.4 66 -14.4 -0.7 -6.1 AGAACTTGCCAGCAAACAAA

4536 SEQ ID N0:4187 -7.1 -20.2 58.5 -13.1 0 -7.2 TTTCCACCGAGCTTGCTTGC

4634 SEQ ID N0:4188 -7.1 -28.3 77.7 -20.6 -0.3 -6.4 CCATCCAGCCTTTAAATGTG

4713 SEQ ID N0:4189 -7.1 -24.2 67.7 -17.1 0 -4.3 AACATCTCGTGAATCAACAG

4748 SEQ ID N0:4190 -7.1 -19.1 57.7 -12 0 -4.6 TTGTAATATTTTTTCTGTTC

4933 SEQ ID N0:4191 -7.1 -17.5 57.3 -10.4 0 -4.2 GCCTATAGTGAAGTAGTAGT

5216 SEQ ID N0:4192 -7.1 -22.2 68.3 -15.1 0 -5.1 GTAGGATTCGGCCAATCCTG

5349 SEQ ID N0:4193 -7.1 -26.3 72.8 -15.3 -3.5 -15.6 CTGCCAGGGTGAATTGTGTC

5656 SEQ ID N0:4194 -7.1 -25.8 74.6 -18.7 0 -3.8 ACACTGAAGTTCTCCAGGAT

5779 SEQ ID N0:4195 -7.1 -23.4 68.8 -15.5 -0.6 -5.6 TTTCCCAAACCTCATAGAAC

5847 SEQ TD N0:4196 -7.1 -21.9 62.7 -14.8 0 -2.8 GACTTTGTTGGGTTTTGCTA

5948 SEQ ID N0:4197 -7.1 -23.5 70.8 -16.4 0 -3.6 CATCCATCTCTCCACTCTCA

6057 SEQ ID N0:4198 -7.1 -27 77.8 -19.9 0 -0.5 TTGCTTTCTTTTTCTGGTTT

6424 SEQ ID N0:4199 -7.1 -22.6 69.9 -15.5 0 -3.6 CACAGAGTTGCAGTGACAGA

6629 SEQ ID N0:4200 -7.1 -23.3 69.4 -16.2 0 -5.5 TTACAATGTTCACTTTGCAC

6746 SEQ ID N0:4201 -7.1 -20.4 62.2 -13.3 0 -5.4 AAAAATATGGCAGATATGGA

6816 SEQ ID N0:4202 -7.1 -16.7 52.4 -8.2 -1.3 -5.2 TGAATTGGGGACCATGGAAA

6856 SEQ ID N0:4203 -7.1 -21.5 61.4 -12.3 -2.1 -8.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGTAGCCATTACAATTCTTT

7014 SEQ ID N0:4204 -7.l -21 63.4 -13 -0.8 -3.7 ATAGAGCTCTGAAACTTAGA

7195 SEQ ID N0:4205 -7.1 -19 59.1 -10.9 0 -10 AGCCTTTAAACTATGTTTAA

7529 SEQ ID N0:4206 -7.1 -18.4 56.9 -9.7 -1.6 -7.4 AGAGCAGGTTGAATTCATTC

7643 SEQ ID N0:4207 -7.1 -21.2 65.1 -13.5 0 -8.4 CAAATTATTCTGCTTGACAT

7670 SEQ ID N0:4208 -7.1 -18.8 57.7 -11.7 0 -3.6 TTTTAGCAAAATATGAAGAA

7802 SEQ ID N0:4209 -7.1 -13.9 47.1 -6.8 0 -4.8 TTTATATTTAAACAAGTTTT

7830 SEQ ID N0:4210 -7.1 -13.8 47.7 -6.7 0 -5.5 ACCATAGTTGGTAGAAAATG

8115 SEQ ID N0:4211 -7.1 -18.5 56.8 -9.5 -1.9 -5.4 TTTAGTGCAGCAGGAATTAA

8219 SEQ ID N0:4212 -7.1 -20.3 61.7 -13.2 0 -6.1 CAATACACTACAGTAAATAG

8374 SEQ ID N0:4213 -7.1 -15.5 50.6 -8.4 0 -3.6 TATCAAACCTGAAAGTTGGA

8713 SEQ ID N0:4214 -7.l -18.8 56.8 -11 -0.4 -4.3 ATCCAAGGGTTCTTGAAAAA

8949 SEQ TD N0:4215 -7.1 -19.3 57.8 -10.1 -2.1 -5.7 TGGTTTTGACCTCACATAAA

8968 SEQ ID N0:4216 -7.1 -20.7 61.5 -11.3 -2.3 -5 TGCATACGAATTACCTTAGA

416 SEQ ID N0:4217 -7 -20.4 60.1 -13.4 0 -4.7 TCTCCATAAATAAATGGAAG

652 SEQ ID N0:4218 -7 -16.1 51.2 -6.8 -2.3 -7.8 AGTAGGGATCCAGGTCCTCC

702 SEQ ID N0:4219 -7 -29.1 83.4 -20.8 -1.2 -8.2 TTGCCCAGGTCCACAAACTC

1084 SEQ ID N0:4220 -7 -27.1 74.2 -20.1 0 -3.6 TGTTCTCAACGCTGAGACAT

1103 SEQ ID N0:4221 -7 -22.8 66.5 -13 -2.8 -8.2 ATGTTCTCAACGCTGAGACA

1104 SEQ ID N0:4222 -7 -22.8 66.5 -13 -2.8 -8.4 TTACCGACTGGATCAGGGCC

1185 SEQ ID N0:4223 -7 -27.6 75.2 -19.7 -0.8 -7.5 CTCCAATGTAATCCTTCCAG

1413 SEQ ID N0:4224 -7 -24 68.3 -17 0 -2.2 AGCCTTCACACAGATGTATC

1517 SEQ ID N0:4225 -7 -23.6 70.1 -15.9 -0.5 -4.2 TGGTAAAGATTTTCCCAATA

1621 SEQ ID N0:4226 -7 -19.7 58.9 -12.7 0 -4.6 GACAAAAAATATCATGTATG

1667 SEQ ID N0:4227 -7 -13.7 46.6 -6.7 0 -4.7 GGCCACCACAGCCAGGATCA

1724 SEQ ID N0:4228 -7 -30.9 82.2 -22.2 -1.7 -8.7 CCTCATAGGCCATGGCCACC

1737 SEQ ID N0:4229 -7 -31.3 82.4 -19.7 -2.5 -17.4 TTCCTTCAAGGTGCTCTCTC

1995 SEQ ID N0:4230 -7 -26 77.6 -18.2 -0.6 -5.3 TCTCCTTTGTTGTTTCCTTC

2008 SEQ ID N0:4231 -7 -25.3 76.4 -18.3 0 0 CATTGGCAAATCTATACCAG

2658 SEQ ID N0:4232 -7 -20.7 61 -13 -0.4 -3.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAGAGGGTATTTAAGACAAT

2779 SEQ ID N0:4233 -7 -17.2 54.5 -10.2 0 -2.9 CTGCTGTGAAAATCCCAGTA

2868 SEQ ID NO:4234 -7 -23.3 66.3 -15.8 -0.1 -3.7 AGTAATAAGGATCCATGGCA

2907 SEQ ID N0:4235 -7 -21.8 64 -14.1 0 -9 AGGCTGACAATAATTCCATC

2953 SEQ ID NO:4236 -7 -21.3 63 -14.3 0 -3.7 CAGCAAAAATGAAGACGATG

3144 SEQ ID N0:4237 -7 -16.5 5l -9.5 0 -4.1 GTGGGAGCGTACAGTCATCA

3219 SEQ ID N0:4238 -7 -26 75.7 -19 0 -4.8 ACAGCACGCGGAACACAATC

3273 SEQ ID NO:4239 -7 -23.6 65 -16.1 0 -7.9 TTTCTAAAAAAGGCTTTTTG

3550 SEQ ID N0:4240 -7 -l6 51.7 -7.6 -1.3 -7.7 TAATCATTTTCATCGATTAC

3721 SEQ ID N0:4241 -7 -17.3 55 -9.6 -0.5 -6.3 CACAATGAAAGTCTCAAACC

4091 SEQ ID N0:4242 -7 -18.5 55.8 -11.5 0.2 -3.1 TAGAACTTGCCAGCAAACAA

4537 SEQ ID N0:4243 -7 -20.6 59.8 -13.6 0 -7.4 TCTGTCATAAAGATGTCTTG

4912 SEQ ID N0:4244 -7 -19.1 60 -12.1 0.2 -6.8 GTAATATTTTTTCTGTTCCT

4931 SEQ ID N0:4245 -7 -20.3 63.2 -13.3 0 -4.2 CTTCCTTTTTAACATAGGCA

5502 SEQ ID N0:4246 -7 -22 65.2 -15 0 -4 AGGTCTCAAAGTTGAACATG

5535 SEQ ID N0:4247 -7 -19.5 59.9 -11.6 -0.8 -6.5 AGGGTGAATTGTGTCAGGGT

5651 SEQ ID NO:4248 -7 -24.7 73.7 -17.7 0 -3.3 TTAACCTTTGCTTTAAAAGA

6213 SEQ TD N0:4249 -7 -17.5 54.4 -10 -0.1 -4.8 GGAGTTCCCATTTAGTTTGT

6311 SEQ ID N0:4250 -7 -24.7 73.5 -17.2 -0.1 -3.4 ACTATCATAGGAAGGAGGAG

6368 SEQ ID N0:4251 -7 -20.3 62.1 -13.3 0 -3.4 AAGATAATTCTTTGTTTCTT

6476 SEQ ID NO:4252 -7 -17.3 56.1 -9.4 -0.8 -3.9 GAAGTCCAGTTGACACATAT

6531 SEQ ID N0:4253 -7 -21.6 64.6 -12.8 -1.8 -4.5 TGAAGTCCAGTTGACACATA

6532 SEQ ID N0:4254 -7 -21.6 64.5 -12.8 -1.8 -5.6 TGTTAAAACAGTCAGTTTGG

6566 SEQ ID N0:4255 -7 -18.7 58.4 -10.5 -1.1 -6.7 GAGAGGTCACTTCACTGTCT

6611 SEQ ID N0:4256 -7 -24.6 74.9 -17.6 0 -3.4 AATTCTTTTTCTGGTTTTGA

7002 SEQ ID N0:4257 -7 -19.7 61.9 -12.7 0 -2.3 ACTGTAGCCATTACAATTCT

7016 SEQ ID NO:4258 -7 -21.9 65.2 -13.9 -0.9 -3.9 ATAACATAGGGTATTTAATT

7368 SEQ ID N0:4259 -7 -16.2 52.6 -9.2 0 -2.9 ATATATATATTTGCCCAAAC

7417 SEQ ID N0:4260 -7 -18.3 55.8 -11.3 0 -6.3 TTAACATGCATTAGTGATAG

7547 SEQ ID N0:4261 -7 -17.8 56.3 -10.8 0 -6.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Tntra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GAAGAATGGATTGAGTAAAA

7586 SEQ ID N0:4262 -7 -15.1 49.4 -8.1 0 -2.1 AGTATCCAGTGTGTTTCCTT

7764 SEQ ID N0:4263 -7 -25.2 75.9 -18.2 0 -3.7 TTTAGCAAAATATGAAGAAT

7801 SEQ ID N0:4264 -7 -13.8 46.8 -6.8 0 -4.2 AACCTGAAAGTTGGAACATG

8708 SEQ ID N0:4265 -7 -18.9 56.6 -11.1 -0.6 -4.8 TTACTACTATTATTATCAAA

8726 SEQ ID N0:4266 -7 -14.7 49.6 -7.7 0 -1.2 ATAAGAGTTTGGTTTTGACC

8977 SEQ ID N0:4267 -7 -20.5 62.8 -11.9 -1.5 -6.3 CCTCTGGCAGCTGTTCAGCT

130 SEQ ID N0:4268 -6.9 -29.7 85 -21 -1.7 -10.8 TTCAGTTTTCTTGCTCCATA

229 SEQ ID N0:4269 -6.9 -23.6 71.3 -16.7 0 -4.4 TCCAAGTCACTATTTGGCTT

619 SEQ ID N0:4270 -6.9 -23.7 69.8 -15.3 -1.4 -5.1 CATATGCCATCACAATGACA

1059 SEQ ID N0:4271 -6.9 -21.7 62.7 -14.1 -0.4 -7.5 TCAGAAAGCTTCTTTACCGA

1198 SEQ ID N0:4272 -6.9 -21.9 63.9 -14.1 -0.7 -6.5 CAATGAGAGCAAACACGCTC

1245 SEQ ID N0:4273 -6.9 -21.3 61.3 -12.2 -2.2 -7.2 GATTGCCCATGAACAGCTGC

1269 SEQ ID N0:4274 -6.9 -26.5 73 -18.9 -0.2 -8.9 TCAGATTGCCCATGAACAGC

1272 SEQ ID N0:4275 -6.9 -24.9 70.1 -18 0 -4.5 AGAATCGCTTGGGGGCCACT

1307 SEQ ID N0:4276 -6.9 -28.3 76.6 -20.7 -0.5 -7 TGGCCACCACAGCCAGGATC

1725 SEQ ID N0:4277 -6.9 -30.2 81.1 -20.9 -2.4 -8.3 CCTGATTCTGCTCCTCATAG

1749 SEQ ID N0:4278 -6.9 -25.7 74.2 -18.8 0 -3.6 TCTGCTTCTTCCAAGGTGGC

1768 SEQ ID N0:4279 -6.9 -27.3 79 -19.5 -0.7 -4.9 CCACTGAAATCTCTTGAAGC

1873 SEQ TD N0:4280 -6.9 -21.4 62.8 -14.5 0 -2.8 GACTGATGAGGGGAGCAGAA

2125 SEQ TD N0:4281 -6.9 -23.5 68.2 -15.9 -0.5 -4.1 CAGATCAAGAACACATTGGC

2671 SEQ ID N0:4282 -6.9 -20.5 61 -13.6 0 -5.4 TTGCCAACTTGAAAACTCTA ' 3039 SEQ ID N0:4283 -6.9 -19.7 58.5 -12.8 0 -3 CAATGATCTTAATTAGCATA

3078 SEQ ID N0:4284 -6.9 -17 54.1 -10.1 0 -4.9 ATTCCCTTTTGCATTCTTCC

3496 SEQ ID N0:4285 -6.9 -26.1 74.9 -19.2 0 -5.1 AGGCTTTTTGGAAACACTCC

3540 SEQ ID N0:4286 -6.9 -23.1 66.9 -15.4 -0.6 -5.4 TCAAACCAGTTGTGCTCAAC

4078 SEQ TD N0:4287 -6.9 -22.4 65.5 -13.3 -2.2 -5.3 ATATATCTTCAAAGGCCAAT

4137 SEQ ID N0:4288 -6.9 -19.5 58.7 -12.1 0 -7.7 CAAGAGCATTGGCTACCAGG

4329 SEQ ID N0:4289 -6.9 -24.9 70.4 -16.2 -1.8 -7 TGATAGAGGGAATTGCTCCA

4458 SEQ ID N0:4290 -6.9 -23.1 67.2 -14.4 -1.8 -5.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CACATTCATGATAGAGGGAA

4466 SEQ ID N0:4291 -6.9 -20 60.5 -13.1 0 -6.l GCAGATGAGGATCATGATGC

5063 SEQ ID N0:4292 -6.9 -23 67.9 -13 -3.1 -8.6 TCATGGTGACCATGTTGAGG

5082 SEQ ID N0:4293 -6.9 -24.1 70.3 -14 -3.2 -11.5 TTGCCCTGGTCATCCGTTTC

5107 SEQ ID N0:4294 -6.9 -29.3 80.9 -21.2 -1.1 -4.4 ACTAGGTTGATCCGGGACAA

5143 SEQ ID N0:4295 -6.9 -24.3 68.6 -16.2 -1.1 -8.5 GGCCGATGTTAAACAACGCA

5430 SEQ ID N0:4296 -6.9 -23.7 64.3 -16.8 2.1 -7.4 TAGCAACACTGAAGTTCTCC

5784 SEQ ID N0:4297 -6.9 -22.4 66.3 -15.5 0 -6.3 GTTGGGTTTTGCTATGAGAA

5942 SEQ ID N0:4298 -6.9 -22.1 66.6 -15.2 0 -3.6 ATAAGCTGGACTTTGTTGGG

5956 SEQ ID N0:4299 -6.9 -22.5 66.8 -15 -0.3 -5.1 TCAAGACAGTGGATCCGGTC

6004 5EQ ID N0:4300 -6.9 -24.9 71.6 -17.1 0 -9.6 GAACTCCCATCTGTTTTTTC

6337 SEQ ID N0:4301 -6.9 -23.5 69.4 -16.6 0 -3.5 GAGGGGTGGTAGAGGAACTC

6351 SEQ ID N0:4302 -6.9 -24.7 73.3 -15.8 -2 -5.5 CTCAAACTTTTCCTTGTCTG

6398 SEQ ID N0:4303 -6.9 -21.9 65.5 -15 0 -2.8 GATAATTCTTTGTTTCTTTT

6474 SEQ ID N0:4304 -6.9 -18.2 58.6 -11.3 0 -2.9 TGGATGCAAGGACTGTACTA

6782 SEQ ID N0:4305 -6.9 -22.2 65.6 -15.3 0 -5.6 GAATGGATGCAAGGACTGTA

6785 SEQ ID N0:4306 -6.9 -21.3 62.9 -14.4 0 -5.6 GACCATGGAAATGCACTAGA

6847 SEQ ID N0:4307 -6.9 -21.8 62.9 -13.5 -1.3 -8.8 GGGGACCATGGAAATGCACT

6850 SEQ ID N0:4308 -6.9 -25.1 69.1 -16.6 -1.5 -8.5 CCTCATTTACAAAAATAGTG

6898 SEQ ID N0:4309 -6.9 -16.9 53.1 -9.3 -0.5 -4.1 GTTTCTTCAACGTAAACCTC

6914 5EQ ID N0:4310 -6.9 -21.2 62.8 -13.7 -0.3 -5.6 GGCAAAACACCTTTGTCTGA

6963 SEQ ID N0:4311 -6.9 -22.9 65.5 -14.4 -1.6 -6.5 TTGCACAACTTTGCCACTTT

8284 SEQ ID N0:4312 -6.9 -23.9 68.2 -16 -0.9 -5 TAACATTAAACTTTTGGTTT

8339 SEQ ID N0:4313 -6.9 -16.3 52.6 -8.2 -1.1 -4.7 GCAATTCCCTGCATGCAGTT

8397 SEQ ID N0:4314 -6.9 -27.5 76.3 -17.5 -1.1 -14.3 TTATTAATTATATATATTTT

8540 SEQ ID N0:4315 -6.9 -12.1 44.3 -5.2 0 -5.2 GTTTGTTGTAAAATTCATGT

8599 SEQ ID N0:4316 -6.9 -17.9 56.9 -11 0.1 -6.6 TCAATTATATTGAAAGAAGA

8804 SEQ ID N0:4317 -6.9 -13.7 47 -5.2 -1.5 -5.6 AAGAGTTTGGTTTTGACCTC

8975 SEQ ID N0:4318 -6.9 -22.1 67 -12,g -2.3 -6.3 ATTATGGGAGAATTATGAAA

9059 SEQ ID N0:4319 -6.9 -15.7 50.9 -g,g 0 -3.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGATGGTTAGGGTATAACGT

155 SEQ ID N0:4320 -6.8 -21.3 63.9 -13.2 -1.2 -5 GATGTGGCTTGGCTTCAGTT

242 SEQ ID N0:4321 -6.8 -26.2 77.5 -18.7 -0.4 -6 GGCTTCTTGGCTTTCTCTTC

562 SEQ ID N0:4322 -6.8 -26.6 80.4 -18.8 -0.9 -3.7 AGGCAGAGGTGGCACTGAAT

774 SEQ ID N0:4323 -6.8 -25.4 73 -17.7 -0.8 -4.7 CAAGGTCATAAATACACAGT

893 SEQ ID N0:4324 -6.8 -18.5 57.1 -11.7 0 -2.8 GCCAAGATTTTTATAAGTGA

976 SEQ ID N0:4325 -6.8 -19.3 59 -12.5 0 -4.1 ATCTTCTAAGCAAAACCCTC

998 SEQ ID N0:4326 -6.8 -21.6 62.9 -14.8 0 -4.l GCCCATGAACAGCTGCAGCC

1265 SEQ ID N0:4327 -6.8 -30.3 80.4 -21 0 -13.2 CACAGAGTAAAGGGTCTTTT

1464 SEQ ID N0:4328 -6.8 -20.9 63.7 -13.4 -0.4 -4.1 ATTCTGCTCCTCATAGGCCA

1745 SEQ ID N0:4329 -6.8 -27.9 79.2 -20.4 -0.5 -7.7 ATGCTGCCGCAACTGCCTGA

1848 SEQ ID N0:4330 -6.8 -29.5 77.4 -21.1 -1.6 -8 GGAGAAAAGGAAGCTGCTTC

2072 SEQ ID N0:4331 -6.8 -21.3 62.9 -12.4 -1 -12.4 GGGGAGCAGAATTTTTTGTC

2116 SEQ ID N0:4332 -6.8 -22.7 68.2 -15.9 0 -3.3 GAGAACCATTTCTGCTGTGA

2879 SEQ ID N0:4333 -6.8 -23.4 68.3 -15.2 -1.3 -5.8 AAAACTCTAAGCAGTCTGAA

3028 SEQ ID N0:4334 -6.8 -18.2 56.3 -11.4 0 -4.1 TGACATATAATCATTTTCAT

3728 SEQ ID N0:4335 -6.8 -16.7 53.8 -9.9 0 -3.4 GTCTCAAACCAGTTGTGCTC

4081 SEQ ID N0:4336 -6.8 -24.7 72.9 -17.2 -0.4 -4.9 AGAAGCATTTCCAGAATGAA

4222 SEQ ID N0:4337 -6.8 -19.5 58.6 -11.7 -0.9 -6 CCATTTGAGAAGCATTTCCA

4229 SEQ ID N0:4338 -6.8 -23.2 66.6 -15.6 -0.6 -4.1 AAATATGTTTGAAATCCATA

4255 SEQ ID N0:4339 -6.8 -15.7 50.6 -8.9 0 -3.3 AAATCTAGCCAGCACCAGGC

4282 SEQ ID N0:4340 -6.8 -26.5 73.4 -18.1 -1.6 -6.1 CCAGGCTAACCAAAGAAACA ' 4314 SEQ ID N0:4341 -6.8 -21.2 59.8 -13 -1.3 -4.2 ACCGGGATAAGGCTCTTAGA

4404 SEQ ID N0:4342 -6.8 -24.6 69.6 -17.3 -0.2 -7.4 GTTGTCATGTTAACACAGTG

4558 SEQ ID N0:4343 -6.8 -20.9 64.4 -13.3 -0.5 -8.7 TACATGTACAGATTTTCTTC

4786 SEQ ID N0:4344 -6.8 -19.1 60.7 -11.6 0 -8.7 ATGATAAAGATGACAAAGTA

4810 SEQ ID N0:4345 -6.8 -14.5 48.4 -7.7 0 -2.8 CCCAAAGATGATAAAGATGA

4817 SEQ ID N0:4346 -6.8 -18 54.2 -11.2 0 -2.1 AATATTTTTTCTGTTCCTCT

4929 SEQ ID N0:4347 -6.8 -20.7 64.1 -13.9 0 -3.8 AAGTTTCTTCATTGCATTGT

4949 SEQ ID N0:4348 -6.8 -21.1 65.1 -14.3 0 -6.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CAAAGACCATTCCTTGGAAT

5013 SEQ ID N0:4349 -6.8 -21.4 61.4 -13 -1.5 -8.1 CCTGGTCATCCGTTTCCACC

5103 SEQ ID N0:4350 -6.8 -30.3 81.4 -22.3 -1.1 -4.5 GGACACAGAATACTTTTCTA

5300 SEQ ID N0:4351 -6.8 -19.6 60.3 -11.8 -0.9 -4.4 GAGGCCGATGTTAAACAACG

5432 SEQ ID N0:4352 -6.8 -21.8 61 -15 3.3 -8.1 CCAACAGATGGGTCCCCACG

5692 SEQ ID N0:4353 -6.8 -29.2 75.5 -21.4 -0.9 -8.8 AAATCCCAACAGATGGGTCC

5697 SEQ ID N0:4354 -6.8 -24.5 67.9 -14.5 -3.2 -7.9 CAATAAGCTGGACTTTGTTG

5958 SEQ ID N0:4355 -6.8 -20.1 60.7 -12.7 -0.3 -4.6 ATATATTTTTTAACCTTTGC

6222 SEQ ID N0:4356 -6.8 -18 56.7 -11.2 0 -3.3 TACTTTACCTTCATAGGCTG

6512 SEQ ID N0:4357 -6.8 -22.6 67.4 -14.6 -1.1 -3.7 ACAAAAATAGTGACATAGCA

6890 SEQ ID N0:4358 -6.8 -16.7 52.6 -9.9 0 -4.1 TAGAGGAGACTTTACAGGCA

7118 SEQ ID N0:4359 -6.8 -22.3 67.1 -14.6 -0.7 -5.2 TCATTCTATCACCAATATGT

7629 SEQ ID N0:4360 -6.8 -20.4 62 -13.6 0 -2.8 TAGCTTCAAATTATTCTGCT

7676 SEQ ID N0:4361 -6.8 -20.1 61.7 -13.3 0 -4.6 AGTCCATGTTATATTTGTAA

8243 SEQ ID N0:4362 -6.8 -19.4 60.6 -12.6 0 -4.3 ATTGCACAACTTTGCCACTT

8285 SEQ ID N0:4363 -6.8 -23.8 67.9 -16 -0.9 -5 TTATTCTTTTGGCTCAATAA

8447 SEQ ID N0:4364 -6.8 -18.8 58.6 -12 0 -4.9 ATATATTTTAAACTGCCAAC

8529 SEQ ID N0:4365 -6.8 -17.1 53.5 -10.3 0 -4.4 GAAAGCTATTGTAGGTGGTT

8744 SEQ ID N0:4366 -6.8 -21.5 65.3 -14.7 0 -5.1 TTTAGATGCTTATAGCCAAG

8780 SEQ TD N0:4367 -6.8 -20.5 62 -12.4 -1.2 -4.6 AGCATCAATTATATTGAAAG

8808 SEQ ID N0:4368 -6.8 -15.7 51.2 -6.9 -2 -8.1 ATAGGAGTTTATATAATGCA

8879 SEQ ID N0:4369 -6.8 -17.8 56.6 -11 0 -5.5 TTCCAGAATCCTCTCTGCTT

17 SEQ ID N0:4370 -6.7 -25.8 74.6 -18.1 -0.9 -4.5 GTTTTCTTGCTCCATAATCT

225 SEQ ID N0:4371 -6.7 -23 69 -16.3 0 -3.6 GGGCCACTGCAAACATTTAT

1295 SEQ ID N0:4372 -6.7 -23.9 67.1 -15.7 -1.4 -7.6 TTCCAGTTAAATGTGCTCAT

1399 SEQ ID N0:4373 -6.7 -21.8 65.1 -14.4 -0.4 -5.6 ATCTCCAATGTAATCCTTCC

1415 SEQ ID N0:4374 -6.7 -23.7 68.4 -17 0 -1.7 CCACAGAGTAAAGGGTCTTT

1465 SEQ ID N0:4375 -6.7 -22.8 67.2 -15.6 -0.2 -3.9 AATGACCAGGACAAAAAATA

1676 SEQ ID N0:4376 -6.7 -15.7 49.6 -9 0 -3.1 CACCAAATAAAATGAGCCCA

1700 SEQ ID N0:4377 -6.7 -20.9 58.5 -14.2 0 -3.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTTCCTTCAAGGTGCTCTCT

1996 SEQ ID N0:4378 -6.7 -25.7 76.2 -18.2 -0.6 -5.3 GTCTTGGGGAAAACAGGGAG

2163 SEQ TD N0:4379 -6.7 -22.6 65.8 -15,9 0 -2.7 TCAGATCCAACATCCTTTGC

2221 SEQ ID N0:4380 -6.7 -24.3 69.8 -17.1 -0.1 -4.5 CAGTGGTGGTGCCCTCTGGG

2484 SEQ ID N0:4381 -6.7 -31.1 86.6 -23.4 -0.9 -5.7 GGCTATGCTCACGGCTCTTT

2579 SEQ ID N0:4382 -6.7 -27.8 78.6 -19.9 -1.1 -5.3 CTATACCAGCATGGCGGACA

2647 SEQ ID N0:4383 -6.7 -26.2 71.8 -18.3 -1.1 -7.4 TCTAAGCAGTCTGAATGATC

3023 SEQ ID N0:4384 -6.7 -20.2 62.4 -13.5 0 -6.3 CTGCATGCCGACCACAGCAA

3158 SEQ ID N0:4385 -6.7 -28.2 74 -20.3 -1.1 -9.1 CTCCCGCATCTTATTTTTCA

3524 5EQ ID N0:4386 -6.7 -25.2 71.5 -18.5 0 -3.1 ATATAATCATTTTCATCGAT

3724 SEQ ID N0:4387 -6.7 -17 54.1 -10.3 0 -6 CCTTCAGATGAGCTGGTTGC

3874 SEQ ID N0:4388 -6.7 -26.3 76.2 -18.4 -1.1 -5 TCGGGTTCAGTTTCAGCTTG

3931 SEQ ID N0:4389 -6.7 -25.3 75 -18.6 0 -4.5 CTTCAGTAAAACAAGCTTCC

3966 SEQ ID N0:4390 -6.7 -20.3 60.7 -13.6 0 -6.4 AGCATTTCCAGAATGAATAT

4219 SEQ ID N0:4391 -6.7 -19.3 58.5 -11 -1.6 -5.2 GAGCATTGGCTACCAGGCTA

4326 SEQ ID N0:4392 -6.7 -27.3 77.1 -18.6 -2 -8.4 GCCTTTAAATGTGGCCACTT

4706 SEQ ID N0:4393 -6.7 -25.3 70.6 -16.2 -1 -13 CAAAACTAGGGTCATGTATT

5126 SEQ TD N0:4394 -6.7 -19.1 58.5 -12.4 0 -4.7 CACTAGGTTGATCCGGGACA

5144 SEQ ID N0:4395 -6.7 -25.7 72 -18.4 -0.3 -6.8 TTGCTCCTTTGATCAGACGT

5367 SEQ ID N0:4396 -6.7 -24.8 71.5 -18.1 0 -6.5 GACATCATCAAAGCAAAGAG

5404 SEQ ID N0:4397 -6.7 -18 55.5 -11.3 0 -4.1 TTGTGTCAGGGTCACAGTCG

5643 SEQ ID N0:4398 -6.7 -25.6 76.1 -17.3 -1.6 -5.8 AAATCAGAGAGTTTAGAGAA ' 5899 SEQ TD N0:4399 -6.7 -16.5 53.6 -9.8 0 -3.2 TGTTGGGTTTTGCTATGAGA

5943 SEQ ID N0:4400 -6.7 -22.8 68.8 -16.1 0 -3.6 GGGCAGATCCATGGCAATAA

5972 SEQ ID N0:4401 -6.7 -24.6 68.9 -16.9 -0.9 -8 ATCAAGACAGTGGATCCGGT

6005 SEQ ID N0:4402 -6.7 -24.5 69.9 -17.1 0 -8.9 CCACTCTCACACAAAACACG

6046 SEQ ID N0:4403 -6.7 -21.8 61.4 -15.1 0 -3 GCTCATAAGAGACTTTGGAG

6120 SEQ ID N0:4404 -6.7 -21.1 64.1 -13 -1.3 -6.3 ATAGCGGCAGACACCTCCTC

6169 SEQ ID N0:4405 -6.7 -28.3 77.8 -21.6 0.4 -6.6 GATAGCGGCAGACACCTCCT

6170 SEQ ID N0:4406 -6.7 -28.5 77.4 -21.3 -0.1 -6.6 kcal) mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGATCACAAAGATAATTCTT

6484 SEQ ID N0:4407 -6.7 -16.3 52.5 -8.4 -1.l -8.2 TATTTGTTAAAACAGTCAGT

6570 SEQ ID N0:4408 -6.7 -17.2 55.3 -10.5 0 -5.9 ATATTAGAGGAGACTTTACA

7122 SEQ ID N0:4409 -6.7 -18.4 58.3 -10.8 -0.7 -5.5 GACATATTTTTAGCAAAATA

7809 SEQ ID N0:4410 -6.7 -15.4 50.5 -6.8 -1.9 -6.6 ATAATTTTCTTATGTACAAA

7886 SEQ ID N0:4411 -6.7 -14.8 49.7 -8.1 0 -6.8 AAATAAATTGTGATGTCATC

7915 SEQ ID N0:4412 -6.7 -15.5 50.9 -8.8 0 -5.1 ACAGTAAATAGTTTTGTAAA

8365 SEQ ID N0:4413 -6.7 -15.1 50.4 -8.4 0 -3.5 CGTAGCTCACCATTCTTTTT

8424 SEQ ID N0:4414 -6.7 -24.2 70.4 -17.5 0 -4.8 TTCTCAATAACTATATCATT

8915 SEQ ID N0:4415 -6.7 -16.5 53.6 -9.8 0 -2.4 CAGCTGTTCAGCTTTTTTTT

123 SEQ ID N0:4416 -6.6 -23.2 70.7 -14.7 -1.8 -11 TTGGCTTCAGTTTTCTTGCT

234 SEQ ID N0:4417 -6.6 -24.8 74.9 -17.5 -0.4 -3.7 TCAAACCTTGATGTGGCTTG

251 SEQ ID N0:4418 -6.6 -22.7 65.7 -15.3 -0.6 -4.9 CATAAATACACAGTTGGTCA

887 SEQ ID N0:4419 -6.6 -19.3 59.1 -12.7 0 -2.8 TCACATATGCCATCACAATG

1062 SEQ ID N0:4420 -6.6 -21.5 62.8 -14.4 -0.1 -6.5 AAAAGCAGAATCGCTTGGGG

1313 SEQ ID N0:4421 -6.6 -21.9 62.3 -13.5 -1.8 -9.3 GGACAAAAAATATCATGTAT

1668 SEQ ID N0:4422 -6.6 -14.9 48.9 -8.3 0 -4.7 GCTCCTCATAGGCCATGGCC

1740 SEQ ID N0:4423 -6.6 -31.5 85.6 -20.9 -1.4 -16.1 TTCTGCTTCTTCCAAGGTGG

1769 SEQ ID N0:4424 -6.6 -25.6 74.9 -18.3 -0.5 -4.6 AATTCGGCCTCTTTTTGTTC

1786 SEQ ID N0:4425 -6.6 -23.9 69.9 -17.3 0 -6.2 CTGCCGCAACTGCCTGAGCT

1845 SEQ ID N0:4426 -6.6 -30.4 79.7 -22.6 -1.1 -7.3 TCATCATCAGCAAAGTCATT

2242 SEQ ID N0:4427 -6.6 -20.8 63.4 -14.2 0 -4.1 AAAATCCCAGTAAAGACCAG

2860 SEQ ID N0:4428 -6.6 -20 58.1 -13.4 0 -2.1 CGTGGGAGCGTACAGTCATC

3220 SEQ ID N0:4429 -6.6 -26.1 74.4 -19 -0.2 -4.8 TCCCACATGGTCTCTATCCA

3301 SEQ ID N0:4430 -6.6 -27.8 78.2 -20.5 -0.5 -4.9 ATAATCATTTTCATCGATTA

3722 SEQ ID N0:4431 -6.6 -17.1 54.5 -9.6 -0.8 -6.3 GCTGGTTGCATTTAATTTCT

3863 SEQ ID N0:4432 -6.6 -22.4 67.2 -14.9 -0.7 -5.1 TTCAGTAAAACAAGCTTCCG

3965 SEQ TD N0:4433 -6.6 -20.2 59.4 -13.6 0 -6.4 AAAGGCCAATGCACCACTAC

4127 SEQ ID N0:4434 -6.6 -24 66.2 -15.9 -1.4 -7.7 AATATATAGGTAAAGACTTT

4204 SEQ ID N0:4435 -6.6 -15 50.1 -7.9 -0.1 -4.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGTTCTGAGTAGCCAAGAGC

4342 SEQ ID N0:4436 -6.6 -24.3 72.7 -17.1 -0.3 -3.4 GTCATGTTAACACAGTGGTA

4555 SEQ TD N0:4437 -6.6 -21.7 66.3 -14.3 -0.5 -8.7 CTAGGTTGATCCGGGACAAA

5142 SEQ ID N0:4438 -6.6 -23.4 66 -15.6 -1.1 -8.2 TTCCAGCCTATAGTGAAGTA

5221 SEQ ID N0:4439 -6.6 -23.3 68.8 -16.7 0 -5.1 GAGAGTTTAGAGAACTCTAT

5893 SEQ ID N0:4440 -6.6 -18.9 60.1 -8.1 -4.2 -9.6 CAGATCCATGGCAATAAGCT

5969 SEQ ID N0:4441 -6.6 -23.1 66.1 -15.1 -1.3 -7.4 CACTGACCATGGGCAGATCC

5982 SEQ ID N0:4442 -6.6 -27.2 75 -18.8 -1.8 -8.8 ATATACTTTACCTTCATAGG

6515 SEQ ID N0:4443 -6.6 -19.6 60.6 -12 -0.9 -4.2 ATACTGTTTCTTCAACGTAA

6919 SEQ ID N0:4444 -6.6 -19.2 59.1 -11:3 -1.2 -5.3 AATGGAAGTAACTGAAACTG

7032 SEQ ID N0:4445 -6.6 -16 51.1 -9.4 0 -3 TCCTTTAAATATTAGAGGAG

7130 SEQ ID N0:4446 -6.6 -17.7 55.9 -9.3 -1.8 -7.4 TAAATTAAATATAGAGCTCT

7205 SEQ ID N0:4447 -6.6 -14.7 49.3 -7.2 0 -9.6 AGCATTTGACCTAAATTAAA

7216 SEQ ID N0:4448 -6.6 -17.1 53.2 -9.9 -0.3 -4 GAGCAGAAGATGGCAAAGCA

7324 SEQ ID N0:4449 -6.6 -22.4 64.7 -14.9 -0.8 -5.6 GAATGATACAGGATACATCC

7508 SEQ ID N0:4450 -6.6 -19.9 59.9 -12.6 -0.5 -6.5 GTGACATTCTTTAACCAAGA

7611 SEQ ID N0:4451 -6.6 -20.1 60.6 -12.6 -0.7 -5.9 TTAGCAAAATATGAAGAATA

7800 SEQ ID N0:4452 -6.6 -13.4 46 -6.8 0 -4.1 AAATGAAATTTATTCTTTTG

8456 SEQ ID N0:4453 -6.6 -13.4 46.5 -5.6 -1.1 -4.7 TTTAAACTGCCAACAGCAAA

8523 SEQ ID N0:4454 -6.6 -19.4 57 -11.2 -1.5 -8.9 TATTAGCCACCAAGTAATTA

8834 SEQ ID N0:4455 -6.6 -20.2 60.2 -13.1 -0.1 -4.1 AATCCTCTCTGCTTATGCCT

11 5EQ ID N0:4456 -6.5 -26.4 75.3 -19 -0.8 -4.6 TCTCTGATAAAACAGAGCCT

208 SEQ TD N0:4457 -6.5 -2l 62.2 -11.5 -3 -8.7 GTGGCTTGGCTTCAGTTTTC

239 SEQ ID N0:4458 -6.5 -26.2 79.1 -19 -0.4 -6 GGTCCCTTCTGTGAATTATC

364 SEQ ID N0:4459 -6.5 -24.4 71.8 -17.9 0 -3.2 GCTTCTTGGCTTTCTCTTCT

561 SEQ ID N0:4460 -6.5 -26.3 79,7 -19.3 -0.1 -3.7 GTAGGGATCCAGGTCCTCCA

701 SEQ ID N0:4461 -6.5 -29.8 84 -21.3 -2 -10.3 ATACAAGGCAGAGGTGGCAC

779 SEQ ID N0:4462 -6.5 -24.5 71 -17.1 -0.8 -4.l TACACAGTTGGTCAAAATAG

881 SEQ ID N0:4463 -6.5 -17.9 56.1 -11.4 0 -3.8 TGACTCAAAGGTATAGATTC

959 SEQ ID N0:4464 -6.5 -18.3 57,g -11.8 0 -2.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCTTGCCAAGATTTTTATAA

980 SEQ ID N0:4465 -6.5 -18.9 58.1 -11.7 -0.4 -6 GTCACATATGCCATCACAAT

1063 SEQ ID N0:4466 -6.5 -22.7 65.9 -16.2 0 -6.5 GGCCCCCACAATGGTCTTTA

1169 SEQ ID N0:4467 -6.5 -29.5 78.6 -22.3 -0.2 -8.5 CAGAAAGCTTCTTTACCGAC

1197 SEQ ID NO:4468 -6.5 -21.7 63 -14.3 -0.7 -7 GCAGCCCAATGAGAGCAAAC

1251 SEQ ID N0:4469 -6.5 -24.8 68.3 -17.5 -0.6 -4.8 AGCCATTTCCACAGAGTAAA

1473 SEQ ID N0:4470 -6.5 -22.8 66 -16.3 0 -3.2 CCACCACAGCCAGGATCAAA

1722 5EQ ID N0:4471 -6.5 -26.5 71.1 -20 0 -5.3 GAAATTCGGCCTCTTTTTGT

1788 SEQ ID N0:4472 -6.5 -23.3 67.1 -16.8 0 -6.7 TTTTGTCACTGGTCAGTCTG

2103 SEQ ID N0:4473 -6.5 -23.7 73 -17.2 0 -6.6 AAAACAGGGAGCCACGGATA

2154 SEQ ID N0:4474 -6.5 -22.8 63.4 -15.7 -0.3 -4.3 GCAATCCACAGTGCTGTGCA

2402 5EQ ID N0:4475 -6.5 -27.4 77 -18.7 -1.7 -12.4 ATGCTGGCTATGCTCACGGC

2584 SEQ ID N0:4476 -6.5 -28.1 78 -20.9 -0.4 -6.5 TGAAAATCCCAGTAAAGACC

2862 SEQ ID N0:4477 -6.5 -19.9 57.9 -13.4 0 -2.1 CTGACAATAATTCCATCAAA

2950 SEQ ID N0:4478 -6.5 -17.6 54 -11.1 0 -2.9 TTAAACTGAGGCTGACAATA

2961 SEQ ID N0:4479 -6.5 -18.5 56.6 -12 0 -3.7 TTCCTAGAGCCCCCACAGAA

3102 SEQ ID N0:4480 -6.5 -29 77.1 -22.5 0 -3.8 AGCTGCATGCCGACCACAGC

3160 SEQ ID N0:4481 -6.5 -30 79.7 -21.2 -2.3 -9.3 ACAGTCATCATTGATCTTGC

3209 SEQ ID N0:4482 -6.5 -22.2 67.8 -15 -0.5 -5.6 AGTTTCAGCTTGTTCACCTT

3923 SEQ ID N0:4483 -6.5 -24.9 74.8 -18.4 0 -4.5 CTTTCGCTGTTCAATGTATA

4154 SEQ ID N0:4484 -6.5 -21.3 64.1 -14.8 0 -2.7 GTAGAACTTGCCAGCAAACA

4538 SEQ ID N0:4485 -6.5 -22.5 64.6 -16 0 -7.4 TTTCTTCATTGCATTGTAAT

4946 SEQ ID N0:4486 -6.5 -19.6 61 -13.1 0 -6.2 GATCATGATGCTGATATCAA

5054 SEQ ID NO:4487 -6.5 -19.7 60 -12.3 -0.6 -8.5 ACCATGTTGAGGCAGATGAG

5074 SEQ ID N0:4488 -6.5 -23.7 69.1 -17.2 0 -4.3 CATCATCAAAGCAAAGAGCA

5402 SEQ ID NO:4489 -6.5 -19.7 58.7 -11.6 -1.6 -4.4 TTCAGTAGCAACACTGAAGT

5789 SEQ ID N0:4490 -6.5 -21 63.7 -11.6 -2.9 -9.6 TTAGAGAACTCTATAAACTG

5887 SEQ ID NO:4491 -6.5 -15.8 51.8 -8.6 -0.3 -8 GTTTAGAGAACTCTATAAAC

5889 SEQ ID N0:4492 -6.5 -16.2 53 -8.1 -1.5 -8.1 GGAGGATCCAGGGCAGCTGC

5920 SEQ ID N0:4493 -6.5 -30.1 84.2 -19.5 -1.8 -16.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CACTCTCACACAAAACACGC

6045 SEQ ID N0:4494 -6.5 -21.6 61.7 -15.1 0 -3.3 AAGAGACTTTGGAGGGGTTT

6114 SEQ TD N0:4495 -6.5 -22.6 67.6 -16.1 0 -2.8 GAGTTCCCATTTAGTTTGTC

6310 SEQ ID N0:4496 -6.5 -23.9 72.5 -17.4 0 -1.7 AGGGGTGGTAGAGGAACTCC

6350 SEQ ID N0:4497 -6.5 -26.1 75.7 -17.2 -2.4 -6.3 TGTTTCTTTTTACTTTTGAT

6464 SEQ TD N0:4498 -6.5 -18.8 60.2 -12.3 0 -2.2 AGAGGTCACTTCACTGTCTT

6610 SEQ ID N0:4499 -6.5 -24.1 73.9 -17.6 0 -3.4 AGAAAATGGAAGTAACTGAA

7036 SEQ ID N0:4500 -6.5 -14.8 48.6 -8.3 0 -3 AGCAATACTTTAGATATATT

7271 SEQ ID N0:4501 -6.5 -16.6 53.9 -10.1 0 -4.3 TATATATATTTGCCCAAACA

7416 SEQ ID N0:4502 -6.5 -19 57 -12.5 0 -5.4 GAAGCTTGCAAAAGACACAT

7482 SEQ ID N0:4503 -6.5 -19.5 58 -10.9 -2.1 -7 TAACATGCATTAGTGATAGC

7546 SEQ ID N0:4504 -6.5 -19.5 60.1 -13 0 -6.8 ATACTCATGTTGAATTAAAA

7721 SEQ ID N0:4505 -6.5 -14.8 49.2 -8.3 0 -4.1 ATACCATATGATACTCATGT

7731 SEQ ID N0:4506 -6.5 -20 60.9 -12.6 -0.8 -6 TAGCAAAATATGAAGAATAG

7799 SEQ ID N0:4507 -6.5 -13.3 45.9 -6.8 0 -4.1 AAGTTTTAGACATATTTTTA

7817 SEQ ID N0:4508 -6.5 -15.8 52.6 -8.6 -0.4 -2.9 CAAAAAACCCCAGAGCCCAA

8494 SEQ ID N0:4509 -6.5 -24.5 63.8 -l8 0 -3.2 TCACAAAGCTGCTTTTAATT

8659 SEQ ID N0:4510 -6.5 -19.4 59 -11.4 -0.3 -10.9 TTGGAACATGTGAACTTGAT

8698 SEQ ID N0:4511 -6.5 -19 57.9 -11.7 -0.6 -6.5 ACCTTATATAATTTATTATG

9073 SEQ ID N0:4512 -6.5 -15.3 50.7 -8 -0.6 -5.3 AATGTCTCCATAAATAAATG

656 SEQ ID N0:4513 -6.4 -15.5 50.1 -8.5 -0.3 -2.9 ATAGTAGGGATCCAGGTCCT

704 SEQ ID N0:4514 -6.4 -26.4 77.1 -18 -2 -8.9 CTACATTCTTTGTCCAGTCA

924 SEQ ID N0:4515 -6.4 -23.7 71.4 -16.7 -0.3 -3.1 AAAACCCTCTTGCCAAGATT

987 SEQ ID N0:4516 -6.4 -22.9 63.8 -15.7 -0.6 -6.3 ACGAAGAAACGTAAAATCTT

1013 SEQ ID N0:4517 -6.4 -15.3 48.8 -8.1 -0.6 -5.6 TGCCCAGGTCCACAAACTCT

1083 5EQ ID N0:4518 -6.4 -27.9 75.7 -21.5 0 -3.6 AACTCTGAATGTTCTCAACG

1112 SEQ ID N0:4519 -6.4 -19.6 59.1 -12.3 -0.8 -5.3 GGAGAACTCTGAATGTTCTC

1116 SEQ ID N0:4520 -6.4 -21 64.3 -10.8 -3.8 -11.4 CCCCACAATGGTCTTTAAAC

1166 SEQ ID N0:4521 -6.4 -23.3 65.1 -16 -0.7 -5.4 AGTTGGGGTTTCGACCAGCC

1533 SEQ ID N0:4522 -6.4 -28.8 79.9 -19.7 -2.7 -7.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCCTCATAGGCCATGGCCAC

1738 SEQ ID N0:4523 -6.4 -29.7 80.9 -18.7 -2.5 -17.4 TTCTTCCAAGGTGGCCTGAT

1763 SEQ ID N0:4524 -6.4 -26.8 75.7 -19.7 -0.4 -7.2 ATTCGGCCTCTTTTTGTTCT

1785 SEQ ID N0:4525 -6.4 -25.5 74.4 -19.1 0 -6.7 GCTCTCCTAACCCACCTACT

1893 SEQ ID N0:4526 -6.4 -29.1 78.6 -22.7 0 -2.8 GCAGAATTTTTTGTCACTGG

2111 SEQ ID N0:4527 -6.4 -21.5 64.9 -14.5 -0.3 -4.1 ATAAAGAGGGTATTTAAGAC

2782 SEQ ID N0:4528 -6.4 -16.2 52.7 -9.8 0 -3 TTGAGAACCATTTCTGCTGT

2881 SEQ ID N0:4529 -6.4 -22.9 67.4 -15.1 -1.3 -5.8 ATCCCTCCACATTTGACAGA

2991 SEQ ID N0:4530 -6.4 -25.6 71.8 -19.2 0 -2.4 TGGAAGAAGTCGTTCATGTG

3244 SEQ ID N0:4531 -6.4 -21 63.1 -13.9 -0.4 -4.9 TATGAATGACATATAATCAT

3734 SEQ ID N0:4532 -6.4 -14.9 49.4 -7.6 -0.7 -5.2 AAACTTTTTAATACACCCTT

3983 SEQ ID N0:4533 -6.4 -18.9 56.9 -12.5 0 -2.5 AAGGCCAATGCACCACTACT

4126 SEQ ID N0:4534 -6.4 -25.6 70.1 -17.7 -1.4 -7.7 TAGTCTTTCGCTGTTCAATG

4158 SEQ ID N0:4535 -6.4 -22 66.5 -15.6 0 -3.1 GTGAAATATGTTTGAAATCC

4258 SEQ ID N0:4536 -6.4 -17.1 53.8 -10.7 0 -4.1 CTTAGAGGTCTTAAAGCTCT

4390 SEQ ID N0:4537 -6.4 -21.4 65.7 -14 -0.9 -5 ACAGTCACTCAAATTGTTAA

4604 SEQ ID N0:4538 -6.4 -18.4 57.5 -12 0 -4.6 ATCCATCCAGCCTTTAAATG

4715 SEQ ID N0:4539 -6.4 -23.4 66.2 -17 0 -4.3 AGCTTCAGCACAAATTCTCC

5182 SEQ ID N0:4540 -6.4 -23.7 69.2 -16.4 -0.7 -6.6 TCCTTTTTAACATAGGCAAA

5500 SEQ ID N0:4541 -6.4 -19.6 58.9 -13.2 0 -4 AAAGTTGAACATGTCATCAA

5528 SEQ ID N0:4542 -6.4 -17.4 54.6 -9.9 -0.8 -9.7 GAGGTTGTAATTTGGAACAA

5575 SEQ ID N0:4543 -6.4 -18.8 57.8 -10.9 -1.4 -7.3 TCCATCCCAGCCAGCAGAGG

5591 SEQ ID N0:4544 -6.4 -31 83.4 -24.6 0 -4.6 AACACTGAAGTTCTCCAGGA

5780 SEQ ID N0:4545 -6.4 -22.7 66.6 -15.5 -0.6 -6.3 CTTTGCTTTAAAAGATAACA

6208 SEQ ID N0:4546 -6.4 -16.1 51.7 -9.2 -0.1 -4.3 AGTTCCCATTTAGTTTGTCA

6309 SEQ ID N0:4547 -6.4 -24 72.2 -17.6 0 -1.7 TCATAGGAAGGAGGAGGGGT

6364 SEQ ID N0:4548 -6.4 -24.3 71.5 -17.9 0 -2.4 TTTGCTTTCTTTTTCTGGTT

6425 SEQ ID N0:4549 -6.4 -22.6 69.9 -16.2 0 -3.6 TCTTTGTTTCTTTTTACTTT

6468 SEQ ID N0:4550 -6.4 -19.6 62.9 -13.2 0 -1.8 ATTTGTTAAAACAGTCAGTT

6569 SEQ ID N0:4551 -6.4 -17.6 56.2 -10.5 -0.4 -5.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GAGTATTTGTTAAAACAGTC

6573 SEQ ID NO:4552 -6.4 -17.1 55.3 -10.7 0 -6.l TGTACTAAAGGTGTTTGGTG

6769 SEQ ID N0:4553 -6.4 -20.5 63 -13.2 -0.8 -5.1 AATGGATGCAAGGACTGTAC

6784 SEQ ID N0:4554 -6.4 -20.9 62.2 -14.5 0 -5.6 AGATTACTTTTTGGAAAGCA

7232 SEQ ID N0:4555 -6.4 -18.8 58.1 -10.8 -1.6 -5.5 AATGGATTTATTAGATTACT

7244 SEQ ID N0:4556 -6.4 -16.3 53.1 -9.9 0 -2.4 AAGAATGGATTGAGTAAAAT

7585 SEQ ID N0:4557 -6.4 -14.5 48.3 -8.1 0 -2.2 ATATTTTTACATTATTTATA

7844 SEQ ID N0:4558 -6.4 -14.1 48.8 -7.7 0 -1.8 TATATTTTTACATTATTTAT

7845 SEQ ID N0:4559 -6.4 -14.1 48.8 -7.7 0 -2.2 CTCAATAATGACGTAGCTCA

8435 SEQ ID NO:4560 -6.4 -20.6 61.3 -13.5 -0.5 -5.6 GGCTCAATAATGACGTAGCT

8437 SEQ ID N0:4561 -6.4 -22.5 65.2 -14.8 -1.2 -6.5 TACCTTATGTATACATAGGA

8893 SEQ ID N0:4562 -6.4 -19.6 60.3 -11 -1.1 -12.6 AATCCAAGGGTTCTTGAAAA

8950 SEQ TD N0:4563 -6.4 -19.3 57.8 -10.8 -2.1 -6.7 TCACATAAATCCAAGGGTTC

8957 SEQ ID N0:4564 -6.4 -20.8 61.9 -14.4 0 -4.3 TTTGACCTCACATAAATCCA

8964 SEQ ID N0:4565 -6.4 -21.3 62 -14.9 0 -2.9 GGAGAATTATGAAATACATA

9053 SEQ TD N0:4566 -6.4 -15 49.4 -7.7 -0.8 -4.5 TCCAGAATCCTCTCTGCTTA

16 SEQ ID N0:4567 -6.3 -25.4 73.6 -18.1 -0.9 -4.5 CTTGGCTTCAGTTTTCTTGC

235 SEQ ID N0:4568 -6.3 -24.8 74.9 -18.5 0 -3.7 CGGAAAAGCTCCAGGTCCCT

377 SEQ ID N0:4569 -6.3 -28 74.2 -20.1 -1.5 -6.7 CGGAAGCTTTCAGGTCCTGG

496 SEQ ID N0:4570 -6.3 -26.8 75 -19 -0.8 -10.8 CTCTGGAGGAATGTCTCCAT

665 SEQ ID N0:4571 -6.3 -24.7 71.9 -15.1 -3.3 -8.5 AATTTTCCTAACAGGGTTTA

812 SEQ ID N0:4572 -6.3 -20.3 61.5 -13.1 -0.7 -4.1 TTCCAGTGAATGTGTACTCT ' 942 SEQ TD N0:4573 -6.3 -22.8 68.6 -16.5 0 -4.9 CTCTGAATGTTCTCAACGCT

1110 SEQ ID N0:4574 -6.3 -22.8 66.4 -15.6 -0.8 -5.3 TTTCAGTGCTCGGAGAACTC

1127 SEQ ID N0:4575 -6.3 -23.6 69.8 -16.4 -0.7 -6.4 AGCCATAGTTGGGGTTTCGA

1539 SEQ ID N0:4576 -6.3 -26.3 75.1 -18.5 -1.4 -7.6 AATTTTTTGTCACTGGTCAG

2107 SEQ ID N0:4577 -6.3 -20.7 64 -14.4 0 -5 GCCACGGATACTCAAGAGAG

2144 SEQ ID N0:4578 -6.3 -23.7 67.3 -16.2 -1.1 -5.7 GTAATAAGGATCCATGGCAA

2906 SEQ ID N0:4579 -6.3 -21.1 61.8 -14.1 0 -9 GTGGGCCAGGATTTTGCCAA

3052 SEQ ID N0:4580 -6.3 -28 76.8 -19.4 -2.3 -7.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGCAAAAATGAAGACGATGA

3143 SEQ ID N0:4581 -6.3 -16.4 5l -10.1 0 -4.1 TCTGCAGATTATTCATTTCA

3468 SEQ ID N0:4582 -6.3 -20.7 64.1 -13.4 -0.2 -9.8 GGCACTGTGACGGTGAGGCT

3763 SEQ ID N0:4583 -6.3 -28.5 79.4 -20.4 -1.8 -7.6 TGCATTTAATTTCTCTTTGC

3857 SEQ ID N0:4584 -6.3 -20.5 63.2 -13.7 -O.l -4.7 TTCAATGTATATATCTTCAA

4145 SEQ ID N0:4585 -6.3 -16.6 54 -10.3 0 -3.9 CCAAAGAAACATCAACGATC

4305 SEQ ID N0:4586 -6.3 -17.8 53.5 -11.5 0 -3.5 TGGCACCGAGTTCTGAGTAG

4350 SEQ TD N0:4587 -6.3 -25.4 73.2 -19.1 0 -4 TCTTAATGTCCGTAATGATT

4373 SEQ ID N0:4588 -6.3 -19.7 59.5 -13.4 0 -2.6 CTAATGTCAAACATGTTACC

4579 SEQ ID N0:4589 -6.3 -18.9 57.6 -11.4 -1.1 -9 CTGGTTGAAGTTATCTATGA~

4871 SEQ ID N0:4590 -6.3 -19.9 61.8 -13.6 0 -2.6 ATTGTAATATTTTTTCTGTT

4934 SEQ TD N0:4591 -6.3 -17.1 55.9 -10.8 0 -3.9 GATCCAAGTTTCTTCATTGC

4954 SEQ ID N0:4592 -6.3 -22.8 68.4 -16.5 0 -4.3 CCTATAGTGAAGTAGTAGTG

5215 SEQ ID N0:4593 -6.3 -20.4 63.6 -14.1 0 -5.1 CAAAGATGGCATAGATAAAC

5469 SEQ ID N0:4594 -6.3 -16.4 51.9 -10.1 0 -4 GCTTCCTTTTTAACATAGGC

5503 SEQ ID N0:4595 -6.3 -23.1 68.3 -16.8 0 -3.6 CAAAGTTGAACATGTCATCA

5529 SEQ ID N0:4596 -6.3 -18.8 57.7 -11.9 -0.3 -7.3 AATAAGCTGGACTTTGTTGG

5957 SEQ ID N0:4597 -6.3 -20.6 62.1 -13.7 -0.3 -5.1 CGCTTTGTAAAGGCAAATAA

6028 SEQ ID N0:4598 -6.3 -18.5 55.4 -10.2 -2 -6.4 CAGACACCTCCTCTTGTTTA

6162 SEQ ID N0:4599 -6.3 -25.1 72.9 -18.8 0 -3 GCAGACACCTCCTCTTGTTT

6163 SEQ ID N0:4600 -6.3 -27.2 77.9 -20.9 0 -3.5 CTTTTCCTTTGCTTTCTTTT

6432 SEQ ID N0:4601 -6.3 -23.2 70.1 -16.9 0 -3.6 AAAGATAATTCTTTGTTTCT

6477 SEQ ID N0:4602 -6.3 -16.5 53.8 -8.4 -1.8 -5.2 TAAAACAGTCAGTTTGGCAT

6563 SEQ ID N0:4603 -6.3 -19.9 60.5 -12 -1.6 -7.7 TCACAGAGTTGCAGTGACAG

6630 SEQ ID N0:4604 -6.3 -23.1 69.7 -15.6 -1.1 -7 GTCAACGTTGATACCCTGCT

6650 SEQ ID N0:4605 -6.3 -26 72.1 -17.4 -0.1 -12.8 CTGGCAAAACACCTTTGTCT

6965 SEQ ID N0:4606 -6.3 -23.2 66.1 -14.6 -2.3 -5.8 ATGGATTTATTAGATTACTT

7243 SEQ ID N0:4607 -6.3 -17.1 55.3 -10.8 0 -2.4 CAGAGCAGGTTGAATTCATT

7644 SEQ ID N0:4608 -6.3 -21.5 64.8 -14.6 0 -8.4 GTTGATTATATTTTTACATT

7851 SEQ ID N0:4609 -6.3 -16.5 54.2 -10.2 0 -2.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAAATAAATTGTGATGTCAT

7916 SEQ ID N0:4610 -6.3 -14.4 48.2 -8.1 0 -5.1 TTAGTGCAGCAGGAATTAAA

8218 SEQ ID N0:4611 -6.3 -19.5 59.3 -13.2 0 -6.6 TAGGTAATTGCACAACTTTG

8291 SEQ ID N0:4612 -6.3 -19.2 58.8 -12.9 0 -5.1 TCAATACACTACAGTAAATA

8375 SEQ TD N0:4613 -6.3 -15.9 51.6 -9.6 0 -3.6 TATTATCAAACCTGAAAGTT

8716 SEQ ID N0:4614 -6.3 -16.8 53.1 -10.5 0.1 -3.1 AGAAGATGAGTTTAGATGCT

8790 SEQ ID N0:4615 -6.3 -19.5 60.8 -13.2 0 -3.6 ACATTTATTAGCCACCAAGT

8839 SEQ ID N0:4616 -6.3 -22.5 65.4 -16.2 0 -3.2 TCTCAATAACTATATCATTA

8914 SEQ TD N0:4617 -6.3 -16.1 52.8 -9.8 0 -2.4 AAATTCTCAATAACTATATC

8918 SEQ ID N0:4618 -6.3 -14.3 48.5 -8 0 -3.1 GAATCCTCTCTGCTTATGCC

12 SEQ ID N0:4619 -6.2 -26.1 74.6 -19 -0.8 -4.6 GCCTTATGAATTACAGCATA

192 SEQ ID N0:4620 -6.2 -21.3 63 -15.1 0 -4.1 ATCCCTGTCAAACCTTGATG

258 SEQ ID N0:4621 -6.2 -24 67.5 -16.6 -1.1 -4.5 TGAATCCTTGACAGGTATCT

279 SEQ ID N0:4622 -6.2 -22.2 66.1 -14.6 -1.3 -3.8 CTTTGGTTTGTTCTCATCAT

602 SEQ ID N0:4623 -6.2 -22.5 69.3 -16.3 0 -1.9 CCTTTATTCATTACTATAAA

739 SEQ TD N0:4624 -6.2 -16.9 53.8 -10.7 0 -3.2 AAACCCTCTTGCCAAGATTT

986 SEQ ID N0:4625 -6.2 -23.7 66.1 -16.7 -0.6 -6.3 GTCCACAAACTCTGTCACAT

1076 SEQ ID N0:4626 -6.2 -23.4 68.2 -16.7 -0.1 -4.2 TTGCCCATGAACAGCTGCAG

1267 SEQ ID N0:4627 -6.2 -26.6 73.1 -19.5 -0.3 -9.4 ACAGAGTAAAGGGTCTTTTT

1463 SEQ ID N0:4628 -6.2 -20.3 62.8 -13.4 -0.4 -4.1 TTGAGTCATGAGTCGAAATA

1598 SEQ ID N0:4629 -6.2 -18.9 58.3 -11 0 -11.6 CAACTGGTAAAGATTTTCCC

1625 SEQ ID N0:4630 -6.2 -21.1 61.8 -14.9 0 -3.3 GAGCATCTGCTGAAATTCGG

1799 SEQ ID N0:4631 -6.2 -22.8 65.6 -15.6 -0.9 -7.9 ACTGGTCAGTCTGTTTCCAT

2096 SEQ ID N0:4632 -6.2 -25.6 76.8 -18.9 -0.1 -7.3 GGGAAAACAGGGAGCCACGG

2157 SEQ ID N0:4633 -6.2 -25.5 68.5 -18.7 -0.3 -4.3 AGTTGTCCAGTAGGTGACGT

2461 SEQ ID N0:4634 -6.2 -25.7 76 -18.4 -l -5.8 AAATTCACAAGATGTTTTAC

2716 SEQ ID N0:4635 -6.2 -16 52.1 -9.8 0 -4.3 TAAAGAGGGTATTTAAGACA

2781 SEQ TD N0:4636 -6.2 -16.9 53.9 -10.7 0 -2.9 CTGAATTGCTCAGTCATGGG

2818 SEQ ID N0:4637 -6.2 -23.4 68.9 -15.9 -1.2 -8.7 GCTGACAATAATTCCATCAA

2951 SEQ ID N0:4638 -6.2 -20.1 59.5 -13.9 0 -2.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CTCCATTAAACTGAGGCTGA

2966 SEQ ID N0:4639 -6.2 -22.6 65.3 -16.4 0 -3.7 TTTTTCACATAATCAATTCC

3511 SEQ ID N0:4640 -6.2 -18.3 57 -12.1 0 -2.5 CTGGTGGTTCCATTCCCATC

3667 SEQ ID N0:4641 -6.2 -28.5 80.1 -21.1 -1.1 -6.6 GGTTGCATTTAATTTCTCTT

3860 SEQ ID N0:4642 -6.2 -21.1 64.9 -14.9 0 -5.1 AACCAGTTGTGCTCAACAAT

4075 SEQ ID N0:4643 -6.2 -22 64 -13.3 -2.5 -5.8 ATATAGGTAAAGACTTTGTC

4201 SEQ ID N0:4644 -6.2 -17.6 56.6 -10.9 -0.1 -4.1 TAAAGCTCTTAATGTCCGTA

4379 SEQ ID N0:4645 -6.2 -20.7 61.5 -14.5 0 -5 GATAGAGGGAATTGCTCCAA

4457 SEQ ID N0:4646 -6.2 -22.4 65.1 -14.4 -1.8 -6.l GTATAAATACATGTACAGAT

4793 SEQ ID N0:4647 -6.2 -16.2 52.6 -9.5 0 -8.2 ATCATGATGCTGATATCAAA

5053 SEQ ID N0:4648 -6.2 -18.4 56.8 -11.3 -0.8 -7.7 AGGCCGATGTTAAACAACGC

5431 5EQ ID N0:4649 -6.2 -23 63.4 -16.8 2.6 -8.1 AGCAATCCATCCCAGCCAGC

5596 SEQ ID N0:4650 -6.2 -30.3 80.9 -24.1 0 -4.1 GTGGAGTTCCCATTTAGTTT

6313 SEQ ID NO:4651 -6.2 -24.7 73.5 -17.2 -l.2 -4.4 GATCACAAAGATAATTCTTT

6483 SEQ ID N0:4652 -6.2 -16.4 52.8 -8.4 -l.8 -8.3 GACTGTACTAAAGGTGTTTG

6772 SEQ ID N0:4653 -6.2 -19.8 61.2 -13.6 0 -5.l TAGCATTATGAATAAACTAT

6875 SEQ ID N0:4654 -6.2 -15 49.6 -8.8 0 -4.1 ATAGCATTATGAATAAACTA

6876 SEQ ID N0:4655 -6.2 -15 49.6 -8.8 0 -4.1 CAGATAGAAACAAACATAAC

7088 SEQ ID N0:4656 -6.2 -13.8 46.6 -7.6 0 -1.2 AATTGACATAGACTATCAGC

7352 SEQ ID N0:4657 -6.2 -18.9 58.8 -12.7 0 -3.3 AAACTATGTTTAATGAATGA

7522 SEQ ID N0:4658 -6.2 -14.3 48.1 -7.6 -0.2 -4.3 ATTTTTAGCAAAATATGAAG

7804 SEQ ID N0:4659 -6.2 -14.1 47.7 -6.8 -1 -5.3 TTAAACAAGTTTTAGACATA

7823 SEQ ID N0:4660 -6.2 -15 50 -8.1 -0.4 -5.5 AGTTGATTATATTTTTACAT

7852 SEQ ID N0:4661 -6.2 -16.4 54 -10.2 0 -2.6 TTTCTTATGTACAAAATGCT

7881 SEQ ID N0:4662 -6.2 -17.7 55.6 -11.5 0 -6.8 TAAATTGTGATGTCATCAAC

7912 SEQ ID N0:4663 -6.2 -17.1 54.5 -9.8 -1 -7.2 AAAAGTTTGGAATTGTGATT

7962 SEQ ID N0:4664 -6.2 -16.4 52.6 -10.2 0 -4.6 TTAGCAATAGCAATTCCCTG

8406 SEQ ID N0:4665 -6.2 -22.5 64.9 -14.6 -1.7 -4.9 TATATATTTTAAACTGCCAA

8530 SEQ ID N0:4666 -6.2 -16.6 52.5 -10.4 0 -4.4 TATATCATTAATACCTTATG

8904 SEQ ID N0:4667 -6.2 -16.2 52.5 -10 0 -4.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAAATATGCATTACAACTGG

9012 SEQ ID N0:4668 -6.2 -16.4 51.7 -10.2 0 -6.5 ATTACAGCATAACAAAGCCC

183 SEQ ID N0:4669 -6.1 -21.5 61.7 -14.9 -0.2 -4.1 GCTCCTTTCCCAGTAAGCCA

307 SEQ ID N0:4670 -6.l -30 81.8 -23.4 -0.1 -3.6 GCACATTTAATTACGTGTAG

441 SEQ ID N0:4671 -6.1 -19.5 59.4 -12.8 -0.3 -5.4 AACAGTGCCTGTGCCATCTT

469 SEQ ID N0:4672 -6.1 -27.2 76.4 -19.5 -1.5 -8.7 TCCTAACAGGGTTTAGTGGA

807 SEQ ID N0:4673 -6.1 -23.7 70 -16.7 -0.7 -4.3 AAGAAACGTAAAATCTTCTA

1010 SEQ ID N0:4674 -6.1 -14.7 48.5 -8.1 -0.2 -6.1 TGAAATCCAGCCAGTTCCAT

1038 SEQ ID N0:4675 -6.1 -25.2 70.3 -19.1 0 -3.2 AAATAAAATGAGCCCAAGAA

1696 SEQ ID N0:4676 -6.1 -16.5 50.8 -10.4 0 -3.2 AAATTCGGCCTCTTTTTGTT

1787 SEQ ID N0:4677 -6.l -22.8 66.2 -16.7 0 -6.7 TGTCTCTCTCTCCTTTGTTG

2016 SEQ ID N0:4678 -6.1 -25.2 77.2 -19.1 0 0 CAAGAGAGACTGATGAGGGG

2132 SEQ TD N0:4679 -6.1 -21.7 64.3 -15.6 0 -2.2 CCTGGATGACATACTGGCCT

2351 SEQ ID N0:4680 -6.1 -26.9 74 -20.8 0 -7.2 TTGCAATCCACAGTGCTGTG

2404 SEQ ID N0:4681 -6.1 -25 71.8 -16.8 -1.5 -12.1 GTAAAGACCAGGTTTCCTAC

2851 SEQ ID N0:4682 -6.1 -22.8 66.9 -16.2 -0.1 -5.9 GCCAACTTGAAAACTCTAAG

3037 SEQ ID N0:4683 -6.1 -18.9 56.6 -12.8 0 -2.9 TGCCAACTTGAAAACTCTAA

3038 SEQ ID N0:4684 -6.1 -18.9 56.4 -12.8 0 -3 AAGTCGTTCATGTGCCACCG

3238 SEQ ID N0:4685 -6.1 -26.8 73.2 -20.7 0 -4.7 GCGACCTCCATACAGTCCCA

3316 SEQ ID N0:4686 -6.1 -30.3 80.1 -23.7 -0.1 -3.4 CATATAATCATTTTCATCGA

3725 SEQ ID N0:4687 -6.1 -17.7 55.4 -11.6 0 -4.8 TGGTTGCATTTAATTTCTCT

3861 SEQ ID N0:4688 -6.1 -21 64.4 -14.9 0 -5.1 CTGGTTGCATTTAATTTCTC ' 3862 SEQ ID N0:4689 -6.1 -21 64.4 -14.9 0 -4.8 GGAAACTTTTTAATACACCC

3985 SEQ ID N0:4690 -6.1 -19.7 58.3 -13.6 0 -2.5 CAAACCAGTTGTGCTCAACA

4077 SEQ ID N0:4691 -6.1 -22.7 65.2 -13.3 -3.3 -6.6 TTCGCTGTTCAATGTATATA

4152 SEQ ID N0:4692 -6.1 -20 61.2 -13.9 0 -3.6 TCTGAGTAGCCAAGAGCATT

4339 5EQ ID N0:4693 -6.1 -23.8 70.1 -16.2 -1.4 -4.1 TTCTGAGTAGCCAAGAGCAT

4340 SEQ ID N0:4694 -6.1 -23.8 70.1 -16.2 -1.4 -4.8 CCACCCTCATGCCTTCAAAC

4422 SEQ ID N0:4695 -6.1 -27.6 73.5 -21.5 0 -4.4 ATTGCTCCAACAAGAGCATT

4447 SEQ ID N0:4696 -6.1 -22.6 65.4 -13.1 -3.4 -9.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TATACAGGCTGAAGTTTAAC

4765 SEQ ID N0:4697 -6.1 -18.7 58.2 -12.6 0 -4.1 CTGTCATAAAGATGTCTTGA

4911 SEQ ID N0:4698 -6.l -19.3 60 -12.5 -0.4 -6.8 CTCTGTCATAAAGATGTCTT

4913 SEQ ID N0:4699 -6.1 -20 62.2 -13.2 -0.4 -6.5 TGTAATATTTTTTCTGTTCC

4932 SEQ ID N0:4700 -6.l -19.4 61.1 -13.3 0 -4.2 TCTTGGATCCAAGTTTCTTC

4959 SEQ ID N0:4701 -6.1 -22.8 69.2 -13 -1.3 -15.5 TGTTGAGGCAGATGAGGATC

5070 SEQ ID N0:4702 -6.l -23 69.1 -16.9 0 -4.1 AGGTTGATCCGGGACAAAAC

5140 SEQ ID N0:4703 -6.l -22.3 63.2 -15 -1.1 -8.7 GGAAGGGACATCATCAAAGC

5410 SEQ ID N0:4704 -6.1 -21.6 63.4 -14.9 -0.3 -3.1 AATTCCAGCTTCCTTTTTAA

5510 SEQ ID N0:4705 -6.1 -22 64.9 -15.9 0 -4.5 CTTCAGTAGCAACACTGAAG

5790 SEQ ID N0:4706 -6.1 -20.7 62.5 -10.6 -4 -11.8 ACTTTCTTCAGTAGCAACAC

5795 SEQ ID N0:4707 -6.1 -21.6 66.1 -14.8 -0.5 -0.8 CTCAAAGTCATCCTCACTCA

5825 SEQ ID N0:4708 -6.l -23.5 69.2 -17.4 0 -2.6 TAAGAGACTTTGGAGGGGTT

6115 SEQ ID N0:4709 -6.1 -22.2 66.6 -16.1 0 -2.9 CTCATAAGAGACTTTGGAGG

6119 SEQ ID N0:4710 -6.1 -20.5 62.5 -12.6 -1.8 -5.4 GAATGATAGCGGCAGACACC

6174 SEQ ID N0:4711 -6.l -24.2 67.6 -18.1 0 -6.6 ATTGCCTCTTTGTTATAGTT

6247 SEQ ID N0:4712 -6.1 -22.3 68.2 -16.2 0 -3 ATTGATCACAAAGATAATTC

6486 SEQ ID N0:4713 -6.1 -15.4 50.7 -8.4 -0.8 -6.3 TTCACAGAGTTGCAGTGACA

6631 SEQ ID N0:4714 -6.l -23.2 69.8 -15.6 -1.4 -7.4 ACCTCATTTACAAAAATAGT

6899 SEQ TD N0:4715 -6.1 -17.1 53.6 -10.4 -0.3 -3.9 CAAAATTAAAGCCATCTAGA

7053 SEQ ID N0:4716 -6.1 -17.1 53 -11 0 -5.8 TTTACAGGCACATAACTGTT

7108 SEQ ID N0:4717 -6.1 -20.8 62.7 -13.1 -1.5 -5.2 TTTAAATATTAGAGGAGACT

7127 SEQ ID N0:4718 -6.1 -16.1 52.6 -9.3 -0.5 -5.7 AAAAGTGCAAAAGTAAAGGT

7705 SEQ ID N0:4719 -6.1 -15.4 49.7 -9.3 0 -4.9 AATAATTTTCTTATGTACAA

7887 SEQ ID N0:4720 -6.1 -14.8 49.7 -8.7 0 -6.8 TTGTGATGTCATCAACCTGA

7908 SEQ ID N0:4721 -6.1 -22.3 66 -15.1 -1 -6.9 ATAAATTGTGATGTCATCAA

7913 SEQ ID N0:4722 -6.1 -16.9 53.9 -9.7 -1 -6.9 ATTATGAAATACATATTTAG

9048 SEQ ID N0:4723 -6.1 -13.2 46.2 -6 -1 -5.2 TTCCAAGTCACTATTTGGCT

620 SEQ ID N0:4724 -6 -23.7 69.8 -16.2 -1.4 -5.1 AATAAATGGAAGGTTCTTTC

644 SEQ ID N0:4725 -6 -17.1 54.5 -11.1 0 -4.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TAGTAGGGATCCAGGTCCTC

703 SEQ ID N0:4726 -6 -26.8 79 -18.8 -2 -8.9 CTGAATCGGAAAATTGCCTT

760 SEQ ID N0:4727 -6 -20.6 59.1 -14.6 0 -4.4 TGGTCAAAATAGTGCACATG

873 SEQ ID N0:4728 -6 -19.7 59.4 -12.7 0 -9.9 TTCTCAACGCTGAGACATTG

1101 SEQ ID N0:4729 -6 -21.7 63.8 -12.9 -2.8 -8.2 TCTTTACCGACTGGATCAGG

1188 SEQ ID N0:4730 -6 -24 68.9 -17.1 -0.7 -5.3 AAAGCAGAATCGCTTGGGGG

1312 SEQ ID N0:4731 -6 -23.8 66.7 -16 -1.8 -9.3 GCCATTTCCACAGAGTAAAG

1472 SEQ ID N0:4732 -6 -22.8 66 -16.8 0 -2.7 CCTTCAAGGTGCTCTCTCTG

1993 SEQ ID N0:4733 -6 -26.4 77.2 -20.4 0 -4.8 GAGAAAAGGAAGCTGCTTCT

2071 SEQ ID N0:4734 -6 -21 62.3 -12.4 -1.8 -13.3 CACTGGTCAGTCTGTTTCCA

2097 SEQ ID N0:4735 -6 -26.3 78 -20.3 0 -6.6 ATCTTCCCATTTGCTGGAAG

2383 SEQ ID N0:4736 -6 -24.5 70.1 -l6 -2.5 -7.5 AGAGCTTAACCTTCTCTTTC

2516 SEQ ID NO:4737 -6 -22.8 69.2 -16 -0.6 -5.2 AGGAATCCTCCAGCATCTCC

2550 SEQ ID N0:4738 -6 -27.7 77.6 -19.8 -1.9 -7.1 TGCTGGCTATGCTCACGGCT

2583 SEQ ID N0:4739 -6 -29 80 -21.8 -1.1 -7.5 GCAAATCTATACCAGCATGG

2653 SEQ ID N0:4740 -6 -22.4 64.6 -14.6 -1.8 -7 AAGACCAGGTTTCCTACAGT

2848 SEQ ID N0:4741 -6 -24.5 71.2 -18 -0.1 -5.9 AAACTGAGGCTGACAATAAT

2959 SEQ ID N0:4742 -6 -18 55 -12 0 -3.7 AACTTGAAAACTCTAAGCAG

3034 SEQ ID N0:4743 -6 -16.9 53.2 -10.9 0 -4.1 ACCGTGGGAGCGTACAGTCA

3222 SEQ ID N0:4744 -6 -27.9 76.9 -21 -0.7 -4.8 CACCGTGGGAGCGTACAGTC

3223 SEQ ID N0:4745 -6 -27.9 76.9 -21 -0.7 -4.8 CCACCGTGGGAGCGTACAGT

3224 SEQ ID N0:4746 -6 -29.5 78.6 -22.6 -0.7 -7.8 CTACTGCAATCTGCAGATTA

3477 SEQ ID N0:4747 -6 -21.7 64.7 -11.3 -4.4 -14 AATTCCCTTTTGCATTCTTC

3497 SEQ ID N0:4748 -6 -23.4 68.8 -17.4 0 -5.1 ATGAATGACATATAATCATT

3733 SEQ TD N0:4749 -6 -15.3 50.2 -7.6 -1.7 -7.9 CAGCAATTGGCACTGTGACG

3771 SEQ ID N0:4750 -6 -24.2 68 -16.6 -1.5 -7.1 CGCTGTTCAATGTATATATC

4150 SEQ ID N0:4751 -6 -19.9 60.8 -13.9 0 -3.9 TCGCTGTTCAATGTATATAT

4151 SEQ ID N0:4752 -6 -19.9 60.8 -13.9 0 -3.8 AGAATGAATATATAGGTAAA

4210 SEQ ID N0:4753 -6 -13.6 46.8 -7.6 0 -4.2 TTGAGAAGCATTTCCAGAAT

4225 SEQ ID N0:4754 -6 -20.3 60.9 -13.5 -0.6 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAGGCTCTTAGAGGTCTTAA

4396 SEQ ID N0:4755 -6 -21.7 66.3 -15.1 -0.3 -5.3 AAGAGCATTCACAACCACCC

4436 SEQ ID N0:4756 -6 -24.9 68.6 -18.3 -0.3 -4.1 CAGCGCCAACATTATCAAAG

4668 SEQ ID N0:4757 -6 -21.5 61.1 -l5 0 -7.6 TAGGTTGATCCGGGACAAAA

5141 SEQ ID N0:4758 -6 -21.8 62.2 -14.6 -1.1 -8.7 GAAGTAGTAGTGTCTGAGGG

5207 SEQ ID NO:4759 -6 -22.4 69.6 -16.4 0 -2.9 GATCCCCTTTGCTCCTTTGA

5375 SEQ TD N0:4760 -6 -28.9 79.3 -22.9 0 -3.6 CCTTTTTAACATAGGCAAAG

5499 SEQ ID N0:4761 -6 -19.2 57.8 -13.2 0 -4 AAGTTGAACATGTCATCAAT

5527 SEQ TD N0:4762 -6 -18.1 56.5 -10.8 -1 -9.9 GGGTGGTGCACTATTAAGAA

5624 SEQ ID N0:4763 -6 -22.3 65.9 -15.1 -1.1 -8.7 TCCCTTAACTGAGCTGCCAG

5669 SEQ ID N0:4764 -6 -27.3 75.2 -20.6 -0.2 -8.8 GACCGCGATGTACATGTTCA

5759 SEQ ID N0:4765 -6 -25 69.5 -18.4 0 -8.4 AAAATCAGAGAGTTTAGAGA

5900 5EQ ID N0:4766 -6 -16.5 53.6 -10.5 0 -4.2 GTTTTGCTATGAGAAGAGGA

5937 SEQ ID N0:4767 -6 -20.8 63.6 -14.8 0 -2.9 ATAAGAGACTTTGGAGGGGT

6116 SEQ TD N0:4768 -6 -22.1 66.2 -16.1 0 -2.9 AGTCCAGTTGACACATATAC

6529 SEQ TD NO:4769 -6 -21.6 65.5 -13.8 -1.8 -5.1 CAGTTTGGCATGGACCTCCT

6554 SEQ ID N0:4770 -6 -28 78.2 -21.5 -0.1 -5.1 GCCATTGGGTTTCTCCTCAG

6704 SEQ ID N0:4771 -6 -28.1 80.5 -21.2 -0.8 -6.3 AACCTCATTTACAAAAATAG

6900 SEQ ID N0:4772 -6 -15.2 49.4 -8.5 -0.5 -3 ATTCTTTTTCTGGTTTTGAG

7001 SEQ ID N0:4773 -6 -20.4 64.4 -14.4 0 -2.3 TAAAGCAATACTTTAGATAT

7274 SEQ ID N0:4774 -6 -15.1 50 -7.5 -1.6 -5.7 TGTGAAGAATGGATTGAGTA

7589 SEQ ID N0:4775 -6 -18.4 57.4 -12.4 0 -2.1 AATAAAGTTGATTATATTTT

7857 SEQ ID N0:4776 -6 -13.3 46.5 -7.3 0 -2.9 TAAAACCCATTATCTAACTT

8022 SEQ ID N0:4777 -6 -17.8 54.4 -11.8 0 -1.6 AATTGCACAACTTTGCCACT

8286 SEQ ID N0:4778 -6 -23 65.4 -16 -0.9 -5 GTTAGGTAATTGCACAACTT

8293 SEQ ID N0:4779 -6 -20.4 61.9 -14.4 0 -5.3 AAAAACAAAAATTTATATCA

8314 SEQ ID N0:4780 -6 -10 39.6 -4 0 -5.2 AAAAAACCCCAGAGCCCAAT

8493 SEQ ID N0:4781 -6 -23.8 62.8 -17.8 0 -3.2 AACAAAAAACCCCAGAGCCC

8496 SEQ ID N0:4782 -6 -24 63.3 -18 0 -3.2 AATTATATATATTTTAAACT

8535 SEQ ID N0:4783 -6 -11.9 43.6 -5.9 0 -5.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGTTTGTTGTAAAATTCATG

8600 SEQ ID N0:4784 -6 -16.7 54.1 -10 -0.4 -5.2 ATTGAAAGCTATTGTAGGTG

8747 SEQ ID N0:4785 -6 -19.1 59.3 -13.1 0 -5.1 TTATATAATGCATTTAAGTA

8871 SEQ ID N0:4786 -6 -15.1 50.4 -8.4 0 -8.8 CTCTGCTTATGCCTCCGCTT

SEQ ID N0:4787 -5.9 -29.4 80.5 -22.6 -0.8 -5 CCAGAATCCTCTCTGCTTAT

SEQ ID N0:4788 -5.9 -25 71.9 -18.1 -0.9 -4.5 CTTACAATATCCCTAGAAGA

335 SEQ ID N0:4789 -5.9 -20.1 60 -14.2 0 -3.8 CCCTTCTGTGAATTATCTCA

36l SEQ ID N0:4790 -5.9 -23.6 68.9 -17.2 -0.1 -3.2 GCATACGAATTACCTTAGAT

415 SEQ ID N0:4791 -5.9 -20.4 60.2 -14.5 0 -3.4 TAGGGATCCAGGTCCTCCAG

700 SEQ ID N0:4792 -5.9 -28.6 80.7 -20.7 -2 -10.3 CTTTCCTTTATTCATTACTA

743 SEQ ID N0:4793 -5.9 -20.1 62.3 -14.2 0 -0.7 TACAAGGCAGAGGTGGCACT

778 SEQ ID N0:4794 -5.9 -25.4 73 -18.6 -0.8 -4.5 ATTTTCCTAACAGGGTTTAG

811 SEQ ID N0:4795 -5.9 -21 63.8 -14.6 -0.1 -3.6 TTCTTTGTCCAGTCAGGAGG

919 SEQ ID N0:4796 -5.9 -25.2 75.8 -17.9 -1.3 -4.7 AATGTTCTCAACGCTGAGAC

1105 SEQ ID N0:4797 -5.9 -21.4 63.2 -13 -2.5 -7.9 ATGAACAGCTGCAGCCCAAT

1261 SEQ ID N0:4798 -5.9 -25.8 70.6 -17.4 0 -13.2 GGCCACTGCAAACATTTATT

1294 SEQ ID N0:4799 -5.9 -22.8 65 -15.4 -l.4 -7 AAAACATAAAAGTGACTGTC

1435 SEQ ID N0:4800 -5.9 -14.9 49.1 -9 0 -6 GTTGGGGTTTCGACCAGCCT

1532 SEQ ID N0:4801 -5.9 -29.7 81.5 -21.9 -1.9 -7 CAGGACAAAAAATATCATGT

1670 SEQ ID N0:4802 -5.9 -15.9 50.7 -10 0 -4.7 GCTGCCGCAACTGCCTGAGC

1846 SEQ ID N0:4803 -5.9 -31.3 82 -24.5 -0.8 -6.4 TACTCAAGAGAGACTGATGA

2136 SEQ TD N0:4804 -5.9 -19.3 59.7 -11.8 -1.5 -5.4 GATACTCAAGAGAGACTGAT

2138 SEQ ID N0:4805 -5.9 -19.3 59.8 -11.8 -1.5 -5.4 TCTGACTTCCGTTTCAGTGG

2498 SEQ ID N0:4806 -5.9 -25.2 73.6 -18.1 -1.1 -5.7 ACAGCAAAAATGAAGACGAT

3145 SEQ ID N0:4807 -5.9 -16.7 51.5 -10.8 0 -4.1 AGAACCACAAGGTTTCCAAT

3379 SEQ ID N0:4808 -5.9 -22 63.2 -13.5 -2.6 -7.6 AGAGGTTCAGAACCACAAGG

3387 SEQ ID N0:4809 -5.9 -22.4 65.6 -13.8 -2.7 -12 GCAGATTATTCATTTCATTG

3465 SEQ ID N0:4810 -5.9 -19.5 60.8 -13.1 -0.2 -3.7 TTTTGGCTTTCTAAAAAAGG

3557 SEQ ID N0:4811 -5.9 -17.1 53.8 -9.7 -1.4 -5.5 CCACTGGTGGTTCCATTCCC

3670 SEQ ID N0:4812 -5.9 -30.3 82.5 -23.2 -1,1 -9.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGAATGACATATAATCATTT

3732 SEQ ID N0:4813 -5.9 -15.4 50.5 -7.6 -1.9 -8.1 TTCAGTTTCAGCTTGTTCAC

3926 SEQ ID N0:4814 -5.9 -23,1 71.7 -17.2 0 -4.5 GTCTTCTTCGGGTTCAGTTT

3938 SEQ ID N0:4815 -5.9 -25.4 77.2 -19.5 0 -3 GATCAAGAAATCTAGCCAGC

4289 SEQ ID N0:4816 -5.9 -21.5 63.4 -15.6 0 -4.7 GAGGGAATTGCTCCAACAAG

4453 SEQ ID N0:4817 -5.9 -22.3 64 -14.6 -1.8 -5.2 TAGAGGGAATTGCTCCAACA

4455 SEQ ID N0:4818 -5.9 -22.7 65.6 -15.7 -1 -6.1 ACAGGCTGAAGTTTAACATC

4762 SEQ ID N0:4819 -5.9 -20.4 62 -14.5 0 -4.5 ATATACAGGCTGAAGTTTAA

4766 SEQ ID N0:4820 -5.9 -18.5 57.7 -12.6 0 -3.9 GTTCCTCTGTCATAAAGATG

4917 SEQ ID N0:4821 -5.9 -21.1 64.1 -14.7 -O.l -3.5 TCATCCGTTTCCACCATCAT

5098 SEQ ID N0:4822 -5.9 -26.8 74.1 -20.9 0 -2.1 ACAAATTCTCCAGTGAACAG

5173 SEQ ID N0:4823 -5.9 -20 60 -14.1 0 -3.9 ACAACGCAGGAAGGGACATC

5418 SEQ ID N0:4824 -5.9 -23.1 65.4 -17.2 0 -3.3 GGTCTCAAAGTTGAACATGT

5534 SEQ ID N0:4825 -5.9 -20.7 62.8 -13.9 -0.8 -6.8 ATTGTGTCAGGGTCACAGTC

5644 SEQ ID N0:4826 -5.9 -24.8 76.3 -17.3 -1.6 -5.8 GCCAGGGTGAATTGTGTCAG

5654 SEQ ID N0:4827 -5.9 -25.6 74.2 -19.7 0 -3.8 GGGCTCTGCACTTTCTTCAG

5804 SEQ ID N0:4828 -5.9 -26.7 78.8 -19.9 -0.7 -6.4 TCTATAAACTGGGTCGCATC

5878 SEQ ID N0:4829 -5.9 -22.1 65 -16.2 0 -3.6 AGATCCATGGCAATAAGCTG

5968 SEQ ID N0:4830 -5.9 -22.4 64.8 -15.1 -1.3 -8 CCGGTCACCACTGACCATGG

5990 SEQ ID N0:4831 -5.9 -29 76.4 -18.7 -4.4 -10.3 GGGTTTGATGCCATAAACCT

6100 SEQ TD N0:4832 -5.9 -24.3 68.1 -15.3 -3.1 -9.5 GCTTTAAAAGATAACATCTG

6204 SEQ ID N0:4833 -5.9 -16.3 52.2 -9.5 -0.8 -6.5 TCTTTCTCAAACTTTTCCTT

6403 SEQ ID N0:4834 -5.9 -21.3 64.7 -15.4 0 -1.7 TATACTTTACCTTCATAGGC

6514 SEQ ID N0:4835 -5.9 -21.4 64.9 -14.3 -1.1 -3.7 TTTCTCCTCAGCAGTGTCAG

6695 SEQ ID N0:4836 -5.9 -25.9 78.3 -20 0 -4.2 AAAAATAGAATGGATGCAAG

6792 SEQ TD N0:4837 -5.9 -14.4 47.6 -8.5 0 -5.6 TTTTTCTGGTTTTGAGCAAA

6997 SEQ ID N0:4838 -5.9 -20.1 61.7 -14.2 0 -5.5 TAGCCATTACAATTCTTTTT

7012 SEQ ID N0:4839 -5.9 -20 61 -14.1 0 -3.2 AAATGGAAGTAACTGAAACT

7033 SEQ ID N0:4840 -5.9 -15.3 49.6 -9.4 0 -3 TATTTGATTTAATAGAAGTT

7449 SEQ ID N0:4841 -5.9 -14.6 49.6 -8.7 0 -2.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GCCTTTAAACTATGTTTAAT

7528 SEQ ID N0:4842 -5.9 -18.4 56.7 -10.4 -2.1 -8 ATTATTTATATTTAAACAAG

7834 SEQ ID N0:4843 -5.9 -12.1 44 -5.6 -0.3 -4.9 ATAAAGTTGATTATATTTTT

7856 SEQ ID N0:4844 -5.9 -14.1 48.5 -8.2 0 -2.6 AATAAATTGTGATGTCATCA

7914 SEQ ID N0:4845 -5.9 -16.9 53.9 -10.1 -0.8 -6.7 AAGTCCATGTTATATTTGTA

8244 SEQ ID N0:4846 -5.9 -19.4 60.6 -13.5 0 -3.8 TGTTAGGTAATTGCACAACT

8294 SEQ ID N0:4847 -5.9 -20.3 61.5 -14.4 0 -6.1 TTCAATACACTACAGTAAAT

8376 SEQ ID N0:4848 -5.9 -16.3 52.5 -10.4 0 -3.6 ATAAAAAATGAAATTTATTC

8461 SEQ ID N0:4849 -5.9 -9.8 39.3 -3 -0.7 -5.2 TTAAACTGCCAACAGCAAAA

8522 SEQ ID N0:4850 -5.9 -18.6 55 -11.2 -1.4 -6.6 TTAATTATATATATTTTAAA

8537 SEQ ID N0:4851 -5.9 -10.6 41 -4.7 0 -6.2 TTTATTAATTATATATATTT

8541 SEQ ID N0:4852 -5.9 -12.1 44.3 -6.2 0 -5.2 ATTATCAAACCTGAAAGTTG

8715 SEQ ID N0:4853 -5.9 -17.1 53.5 -10.5 -0.4 -3.6 TAGGAGTTTATATAATGCAT

8878 SEQ ID N0:4854 -5.9 -17.8 56.6 -11.9 0 -7 ACATAAATCCAAGGGTTCTT

8955 SEQ ID N0:4855 -5.9 -20.7 61.6 -14.8 0 -3.8 ATCCTCTCTGCTTATGCCTC

SEQ ID N0:4856 -5.8 -27.5 79.7 -20.8 -0.8 -4.6 TCTCTGTGGATAAGAAAACA

46 SEQ ID N0:4857 -5.8 -18 55.9 -12.2 0 -2.8 AGCATCCAAGATGGTTAGGG

163 SEQ ID N0:4858 -5.8 -24.1 70 -16.8 -1.4 -6.2 CCTTTCCCAGTAAGCCACTC

304 SEQ ID N0:4859 -5.8 -28.4 78.1 -22.6 0 -3.4 TCCTTTCCCAGTAAGCCACT

305 SEQ ID N0:4860 -5.8 -28.4 78.1 -22.6 0 -3.4 CTCCTTTCCCAGTAAGCCAC

306 SEQ ID N0:4861 -5.8 -28.4 78.1 -22.6 0 -3.2 TACAATATCCCTAGAAGAGA

333 SEQ ID N0:4862 -5.8 -19.7 59.2 -13.9 0 -3.8 AGGTCCCTTCTGTGAATTAT

365 SEQ ID N0:4863 -5.8 -24 70.4 -18.2 0 -4 AAAAGCTCCAGGTCCCTTCT

374 SEQ ID N0:4864 -5.8 -26.8 74.4 -21 0 -5 GAACTCTGAATGTTCTCAAC

1113 SEQ ID N0:4865 -5.8 -19.4 59.8 -12.3 -1.2 -6.2 TACCGACTGGATCAGGGCCC

1184 SEQ ID N0:4866 -5.8 -29.5 78.2 -22.1 -0.8 -11.1 TTTACCGACTGGATCAGGGC

1186 SEQ ID N0:4867 -5.8 -25.7 72.1 -19 -0.8 -5.3 TGTCATCTCCAATGTAATCC

1419 SEQ ID N0:4868 -5.8 -22.6 66.7 -16.2 -0.3 -3 TCTCTCCTGCTTTCGCCGTC

2278 SEQ ID N0:4869 -5.8 -30.2 83.5 -23.7 -0.5 -3.6 CATACTGGCCTGACTAACGT

2342 SEQ ID N0:4870 -5.8 -24.3 68 -18.5 0 -7.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CAAATCTATACCAGCATGGC

2652 SEQ ID N0:4871 -5.8 -22.4 64.6 -14.6 -2 -7.3 TACAGTCATCATTGATCTTG

3210 SEQ ID N0:4872 -5.8 -20.1 62.7 -14.3 0 -5.6 CCGTGGGAGCGTACAGTCAT

3221 SEQ ID N0:4873 -5.8 -27.7 76.3 -2l -0.7 -4.8 CTCCATACAGTCCCACATGG

3311 SEQ ID N0:4874 -5.8 -27 74.8 -20.3 -0.8 -5.2 AAAAAAGGCTTTTTGGAAAC

3545 SEQ ID N0:4875 -5.8 -15.4 49.6 -8.2 -1.3 -7.7 AGCAGGTTTTTCGAAGATTC

4050 SEQ ID N0:4876 -5.8 -21.5 65 -15 -0.4 -6.8 TGTTCAATGTATATATCTTC

4147 SEQ ID N0:4877 -5.8 -17.8 57.7 -12 0 -3.9 AAAGCTCTTAATGTCCGTAA

4378 SEQ ID N0:4878 -5.8 -20.3 60.1 -14.5 0 -5 ATACATAATATCCATCCAGC

4724 SEQ ID N0:4879 -5.8 -21.3 62.8 -15.5 0 -2.8 GCTGGTTGAAGTTATCTATG

4872 SEQ ID N0:4880 -5.8 -21.1 64.8 -15.3 0 -2.8 CCACCATCATGGTGACCATG

5088 SEQ ID NO:4881 -5.8 -26.6 72.6 -17.1 -3.7 -11.1 TCATCTCAGCCAGAAACATA

5280 SEQ ID N0:4882 -5.8 -21.8 64.2 -16 0 -3.6 GCAATAAGCTGGACTTTGTT

5959 SEQ ID N0:4883 -5.8 -21.9 64.9 -15.4 -0.5 -5.l TATAGTTACTTGATATATTT

6234 SEQ ID N0:4884 -5.8 -15.8 52.8 -10 0 -4.3 CTTTGCTTTCTTTTTCTGGT

6426 SEQ ID N0:4885 -5.8 -23.4 71.6 -17.6 0 -3.6 ATACTTTACCTTCATAGGCT

6513 SEQ ID N0:4886 -5.8 -22.6 67.5 -15.6 -1.1 -3.7 TACAAAAATAGTGACATAGC

6891 SEQ ID N0:4887 -5.8 -15.7 50.9 -9.9 0 -3.5 CATTTACAAAAATAGTGACA

6895 SEQ ID N0:4888 -5.8 -15.1 49.4 -8.6 -0.5 -4.8 ACCTAAATTAAATATAGAGC

7208 SEQ ID N0:4889 -5.8 -15.6 50.6 -9.8 0 -3 TGTGACATTCTTTAACCAAG

7612 SEQ ID N0:4890 -5.8 -19.5 59.2 -13.7 0 -2.9 CATAAAGTAAAATAAATTGT

7924 SEQ TD N0:4891 -5.8 -11.4 42.2 -5.6 0 -3.2 CACAACTTTGCCACTTTGTT

8281 SEQ ID N0:4892 -5.8 -23.3 67.3 -17 -0.2 -3.8 CTTTTGGCTCAATAATGACG

8442 SEQ ID N0:4893 -5.8 -20.1 59.5 -13.6 -0.5 -5.3 TTATGGGAGAATTATGAAAT

9058 SEQ ID N0:4894 -5.8 -15.7 50.9 -9.9 0 -3.2 CTATAAAAGTTTTCTTATTG

726 SEQ ID N0:4895 -5.7 -14.9 50.2 -8.1 -l -6.3 TGTACTCTACATTCTTTGTC

930 SEQ ID N0:4896 -5.7 -21.1 66.5 -14.7 -0.4 -5.4 GGTCTTTTTGCCCATCCAAA

1452 SEQ ID N0:4897 -5.7 -26.3 72.8 -20.1 -0.2 -3.5 AATAAAATGAGCCCAAGAAA

1695 SEQ ID N0:4898 -5.7 -16.5 50.8 -10.8 0 -3.2 ATTGCGTCTTGGGGAAAACA

2168 SEQ ID N0:4899 -5.7 -22.3 63.7 -16.6 0 -5.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAGTTGTCCAGTAGGTGACG

2462 SEQ ID N0:4900 -5.7 -23.8 69.9 -17.4 -0.4 -5.2 GGTATTTAAGACAATGCAAA

2774 SEQ ID N0:4901 -5.7 -17.2 53.8 -11 -0.1 -5.6 TAAACTGAGGCTGACAATAA

2960 SEQ ID N0:4902 -5.7 -17.7 54.5 -12 0 -3.7 GTCGTTCATGTGCCACCGTG

3236 SEQ ID N0:4903 -5.7 -28.7 78.4 -23 0 -4.7 ATCAATTCCCTTTTGCATTC

3500 SEQ ID N0:4904 -5.7 -23.1 67.6 -17.4 0 -5.1 TTCAAAGGCCAATGCACCAC

4130 SEQ TD NO:4905 -5.7 -24.4 67.2 -17.2 -1.4 -7.7 CTTTGTCAGCATATTCTAGC

4188 SEQ ID N0:4906 -5.7 -22.5 69.1 -16.8 0 -4.1 CTGAGTAGCCAAGAGCATTG

4338 SEQ ID N0:4907 -5.7 -23.4 68.3 -16.2 -1.4 -4.6 CCAACAAGAGCATTCACAAC

4441 SEQ ID N0:4908 -5.7 -20.9 60.8 -14.6 -0.3 -4.1 GACAGTCACTCAAATTGTTA

4605 SEQ TD N0:4909 -5.7 -19.7 60.8 -14 0 -5.7 ACATCTCGTGAATCAACAGC

4747 SEQ ID N0:4910 -5.7 -21.6 63.6 -15.9 0 -4.6 GGTTGAAGTTATCTATGATG

4869 SEQ ID N0:4911 -5.7 -19 59.8 -13.3 0 -3.2 ACAACCAGGAAGGATATGAT

5737 SEQ ID N0:4912 -5.7 -20.3 59.9 -13.8 -0.6 -4.5 CTCCAGGATGACCGCGATGT

5768 SEQ ID N0:4913 -5.7 -27.8 74.1 -21.4 -0.5 -7.2 TCAGTAGCAACACTGAAGTT

5788 SEQ ID N0:4914 -5.7 -21 63.7 -13.4 -1.9 -7.6 CAAGACAGTGGATCCGGTCA

6003 SEQ ID N0:4915 -5.7 -25.2 71.1 -17.9 -1.1 -10.8 CGCTGAATGATAGCGGCAGA

6178 SEQ ID N0:4916 -5.7 -24.6 67.7 -16.2 -2.7 -9.5 TTTTAACCTTTGCTTTAAAA

6215 SEQ ID N0:4917 -5.7 -17.1 53.6 -10.5 -0.7 -4.3 TTTTTTCTGGAGTGGAGTTC

6324 SEQ ID N0:4918 -5.7 -22.3 69.7 -16.6 0 -2.4 TTGTTTCTTTTTACTTTTGA

6465 SEQ ID NO:4919 -5.7 -18.9 60.6 -13.2 0 -2.2 TAGGCTGTAAACAATTGATC

6499 SEQ ID N0:4920 -5.7 -18.4 57.1 -12.7 0 -7.2 TTTACCTTCATAGGCTGTAA

6509 SEQ ID N0:4921 -5.7 -22 65.8 -15.1 -1.1 -4.5 TTTGTTAAAACAGTCAGTTT

6568 SEQ ID N0:4922 -5.7 -17.7 56.6 -10.5 -1.4 -5.9 TCCTCAGCAGTGTCAGCTGG

6691 SEQ ID N0:4923 -5.7 -28.3 82.9 -19.8 -2.8 -9.2 TTGTAAAAATATGGCAGATA

6820 SEQ ID N0:4924 -5.7 -15.9 51.2 -10.2 0 -4 AAAATAGTGACATAGCATTA

6887 SEQ ID N0:4925 -5.7 -16.3 52.4 -10.6 0 -4.1 AAGCATTTGACCTAAATTAA

7217 SEQ ID N0:4926 -5.7 -17.1 53.2 -10.8 -0.3 -4 ATATGTGACATTCTTTAACC

7615 SEQ ID N0:4927 -5.7 -19.2 59.2 -13.5 0 -3.9 TTGAATTAAAAGTGCAAAAG

7712 SEQ ID N0:4928 -5.7 -13.3 45.7 -7.6 0 -5.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAAATATGAAGAATAGGTTT

7795 SEQ ID N0:4929 -5.7 -13.7 46.8 -8 0 -2.7 TAAAGTAAAATAAATTGTGA

7922 SEQ ID N0:4930 -5.7 -11.3 42.1 -5.6 0 -3.2 TCAAAAGTTTGGAATTGTGA

7964 SEQ ID N0:4931 -5.7 -17.4 54.7 -11.2 -0.1 -6.9 GAAATTATCCATTGAAAAAT

7993 SEQ ID N0:4932 -5.7 -14 46.8 -7.7 -0.3 -3.8 GTGTTTGACCAATGTATCTC

8083 SEQ ID N0:4933 -5.7 -22 66.7 -16.3 0 -3.4 AAAAACATCTATGAATAAAA

8150 SEQ ID N0:4934 -5.7 -10.6 40.6 -4.9 0 -2.9 AAAAAACATCTATGAATAAA

8151 SEQ ID N0:4935 -5.7 -10.6 40.6 -4.9 0 -3.8 GCAATAAAAAATGAAATTTA

8464 SEQ ID N0:4936 -5.7 -ll.l 41.5 -4.1 -1.2 -5.7 ATTTTAAACTGCCAACAGCA

8525 SEQ ID N0:4937 -5.7 -20.9 60.9 -13.6 -1.5 -7.3 TGAAAGCTATTGTAGGTGGT

8745 SEQ ID N0:4938 -5.7 -21.4 64.8 -15.7 0 -5.l CTCTGATAAAACAGAGCCTT

207 SEQ ID N0:4939 -5.6 -20.7 61.2 -12.7 -2.4 -7.6 ATCTCTGATAAAACAGAGCC

209 SEQ ID N0:4940 -5.6 -20.1 60.3 -11.5 -3 -8.7 TCTTGCTCCATAATCTCTGA

221 SEQ ID N0:4941 -5.6 -23.4 69.4 -17.8 0 -3.6 CTTCAGTTTTCTTGCTCCAT

230 SEQ ID N0:4942 -5.6 -24.8 74.1 -19.2 0 -4.4 TCCCTTCTGTGAATTATCTC

362 SEQ ID N0:4943 -5.6 -23.3 69.4 -17.2 -0.1 -3.2 GTTTTTCGATAGCAGCAAGA

534 SEQ ID N0:4944 -5.6 -22 65.4 -16.4 0 -5.4 TGTTCTCATCATCATTATCT

594 SEQ ID N0:4945 -5.6 -21 65.8 -15.4 0 -1.7 ATGGAAGGTTCTTTCCAGCT

639 SEQ ID N0:4946 -5.6 -24.9 72.9 -16.2 -3.1 -9.4 TTCCTAACAGGGTTTAGTGG

808 SEQ ID N0:4947 -5.6 -23.2 69 -16.7 -0.7 -4.1 GTACTCTACATTCTTTGTCC

929 SEQ ID N0:4948 '5.6 -23.1 70.8 -16.9 -0.3 -4.6 CGAAGAAACGTAAAATCTTC

1012 SEQ ID N0:4949 -5.6 -15.5 49.4 -8.1 -1.8 -8 AAGCTTCTTTACCGACTGGA

1193 SEQ ID N0:4950 -5.6 -23.8 67.9 -17.3 -0.7 -7.3 TCCTTCCAGTTAAATGTGCT

1402 SEQ ID NO:4951 -5.6 -24 69.6 -18.4 0 -5.6 TCGGCCTCTTTTTGTTCTGC

1783 SEQ ID N0:4952 -5.6 -27.2 78.4 -21.6 0 -6.7 CTGAAATCTCTTGAAGCAGC

1870 SEQ ID N0:4953 -5.6 -21 62.8 -15.4 0 -4.1 AAGAGAGACTGATGAGGGGA

2131 SEQ ID N0:4954 -5.6 -21.6 64.4 -16 0 -2.2 TGCGGCACAAACAGTGAGTC

2296 SEQ ID N0:4955 -5.6 -24.1 68.6 -17.6 -0.8 -7.2 CACCATTGCAATCCACAGTG

2409 SEQ ID N0:4956 -5.6 -24.7 69.1 -18.5 0 -8.6 TCTATACCAGCATGGCGGAC

2648 SEQ ID N0:4957 -5.6 -25.9 72.3 -18.3 -2 -7.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CCGGTTTAAAGTCTTCTTCG

3948 SEQ ID N0:4958 -5.6 -22.9 66 -16.6 -0.5 -6.2 TTTGAGAAGCATTTCCAGAA

4226 SEQ ID N0:4959 -5.6 -20.4 61.2 -14.2 -0.3 -4.1 TCAAACCGGGATAAGGCTCT

4408 SEQ TD N0:4960 -5.6 -23.9 66.6 -18.3 0 -6.2 TCCAGCCTTTAAATGTGGCC

4710 SEQ ID N0:4961 -5.6 -26.5 73.1 -19.1 -l.8 -8.6 TTTGCTCCTTTGATCAGACG

5368 SEQ ID N0:4962 -5.6 -23.7 68.6 -18.1 0 -6.5 CACCACAACCAGGAAGGATA

5741 SEQ ID N0:4963 -5.6 -23.3 65 -16.9 -0.6 -4.3 GCATCGGGATCAAACTTTTC

5863 SEQ ID N0:4964 -5.6 -22.2 64.7 -16.6 0 -5.3 AGGCTGTAAACAATTGATCA

6498 SEQ ID N0:4965 -5.6 -19.4 58.8 -13.8 0 -7.2 CTTTGCACAACTATCCCTAT

6734 SEQ ID N0:4966 -5.6 -23.6 67.4 -18 0 -5 TGTAAAAATATGGCAGATAT

6819 SEQ ID N0:4967 -5.6 -15.8 50.9 -10.2 0 -4.4 CAATATGTGACATTCTTTAA

7617 SEQ TD N0:4968 -5.6 -17 54.2 -11.4 0 -3.9 TCAAATTATTCTGCTTGACA

7671 SEQ ID N0:4969 -5.6 -19.2 59 -13.6 0 -3.6 TGGAATTGTGATTAAAAATC

7955 SEQ ID N0:4970 -5.6 -14.4 48.2 -8.1 -0.5 -4.l AAAGTTTGGAATTGTGATTA

7961 SEQ ID N0:4971 -5.6 -16.8 53.8 -11.2 0 -3.7 AAGAAGATGAGTTTAGATGC

8791 SEQ ID N0:4972 -5.6 -17.9 56.7 -12.3 0 -2.6 CATCAATTATATTGAAAGAA

8806 SEQ ID N0:4973 -5.6 -13.8 47 -6.2 -2 -6.6 TAGCATCAATTATATTGAAA

8809 SEQ TD N0:4974 -5.6 -15.4 50.5 -8.2 -1.5 -8.1 TAAATTCTCAATAACTATAT

8919 SEQ ID N0:4975 -5.6 -13.6 46.9 -8 0 -3.1 ATAAATTCTCAATAACTATA

8920 SEQ ID N0:4976 -5.6 -13.6 46.9 -8 0 -3.1 TATAAATTCTCAATAACTAT

8921 SEQ ID N0:4977 -5.6 -13.6 46.9 -8 0 -3.l ACAATTTTTTTTCTTTCTTT

65 SEQ ID N0:4978 -5.5 -17.9 57.7 -12.4 0 -2.6 CATCCCTGTCAAACCTTGAT

259 SEQ ID N0:4979 -5.5 -24.7 68.8 -18 -l.1 -4.5 TTGATAGCAATTTTCCTAAC

820 SEQ ID N0:4980 -5.5 -19 58.3 -13 -O.l -0.2 GTTGCTCAAGGTCATAAATA

899 SEQ ID N0:4981 -5.5 -19.9 60.9 -14.4 0 -3.6 TACATTCTTTGTCCAGTCAG

g23 SEQ ID N0:4982 -5.5 -22.8 69.6 -16.7 -0.3 -3.1 TCCAGTGAATGTGTACTCTA

941 SEQ ID N0:4983 -5.5 -22.4 67.6 -16.9 0 -4.9 CTCTGTCACATATGCCATCA

1067 SEQ ID N0:4984 -5.5 -24.7 71.9 -19.2 0 -6.5 GAAATTGTTTTCAGTGCTCG

1135 SEQ ID N0:4985 -5.5 -21 63 -14 -1.4 -6.4 AACAAATGTCCCATTTGAAT

1370 SEQ ID N0:4986 -5.5 -19.2 56.9 -9.9 -3.8 -10.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTATCCTTCTGGACACTGGC

1502 SEQ ID N0:4987 -5.5 -26.4 76.6 -19.5 -1.3 -5.3 GCTTGTGTAGCCATAGTTGG

1547 SEQ ID N0:4988 -5.5 -25.6 75.5 -19 -1 -4.8 GTCATGAGTCGAAATAGAGA

1594 SEQ ID N0:4989 -5.5 -19.4 59.5 -13.9 0 -6.6 AGTCATGAGTCGAAATAGAG

1595 SEQ ID N0:4990 -5.5 -18.8 58.4 -13.3 0 -6.6 CCAGGACAAAAAATATCATG

1671 SEQ ID N0:4991 -5.5 -16.7 51.7 -11.2 0 -4.1 GAAAAGGAAGCTGCTTCTTT

2069 SEQ ID N0:4992 -5.5 -20.6 61.5 -12.4 -1.8 -13.5 AGAAAAGGAAGCTGCTTCTT

2070 SEQ ID N0:4993 -5.5 -20.5 61.3 -12.4 -1.8 -13.3 AGACTGATGAGGGGAGCAGA

2126 SEQ ID N0:4994 -5.5 -24.2 70.8 -18 -0.5 -4.1 ATGTTTTACTTTTAACCATG

2705 SEQ ID N0:4995 -5.5 -18.5 57.6 -13 0 -3.9 GTATTTAAGACAATGCAAAT

2773 SEQ ID N0:4996 -5.5 -16 51.4 -10 -0.1 -5.6 CAGGTTTCCTACAGTCAACA

2843 SEQ TD N0:4997 -5.5 -23.7 69.8 -17.7 -0.2 -3.9 TGAGAACCATTTCTGCTGTG

2880 5EQ ID N0:4998 -5.5 -22.8 66.9 -15.9 -1.3 -5.8 GCAATGATCTTGAGAACCAT

2890 SEQ ID N0:4999 -5.5 -21.2 62.1 -15 -0.4 -6.2 TCCATTAAACTGAGGCTGAC

2965 SEQ ID N0:5000 -5.5 -21.9 64 -16.4 0 -3.7 GAGGTTCAGAACCACAAGGT

3386 SEQ ID N0:5001 -5.5 -23.6 68.5 -15.4 -2.7 -12 TGTCATCATCAGTAGCAGCA

3447 SEQ ID N0:5002 -5.5 -24.4 73.7 -18.9 0 -5.4 CACTACTGAGAAGGATCATG

4113 SEQ ID N0:5003 -5.5 -20 60.7 -13.8 -0.5 -5.9 CAGAATGAATATATAGGTAA

4211 SEQ ID N0:5004 -5.5 -15 49.7 -9.5 0 -4.2 GAGAAGCATTTCCAGAATGA

4223 SEQ ID N0:5005 -5.5 -20.8 61.8 -13.7 -1.6 -6 GTCCGTAATGATTTGATGGC

4366 SEQ ID N0:5006 -5.5 -23 66.5 -17.5 0 -3.3 GCAGTGCAAGATAGCCAGCG

4683 SEQ ID N0:5007 -5.5 -26.9 74.4 -20.7 -0.2 -8.9 CATATACAGGCTGAAGTTTA

4767 SEQ ID N0:5008 -5.5 -19.9 61 -14.4 0 -4.1 AAAGATGTTCCAGCCTATAG

5228 SEQ ID N0:5009 -5.5 -21.7 63.9 -16.2 0 -4.7 ACCAGGAAGGATATGATGAT

5734 SEQ ID NO:5010 -5.5 -20.7 61.3 -14.4 -0.6 -4 TTTAGAGAACTCTATAAACT

5888 SEQ ID N0:5011 -5.5 -15.9 52.1 -9.7 -0.3 -8 TTGGAGGGGTTTGATGCCAT

6106 SEQ ID N0:5012 -5.5 -26 74 -19.4 -1 -4.8 GGTGGTAGAGGAACTCCCAT

6347 SEQ ID N0:5013 -5.5 -26.4 74.8 -18 -2.9 -8.2 CACTATCATAGGAAGGAGGA

6369 SEQ ID N0:5014 -5.5 -21 63.1 -15.5 0 -3.4 TTGTTAAAACAGTCAGTTTG

6567 SEQ TD N0:5015 -5.5 -17.6 56.2 -10.5 -1.6 -7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GACTATGAGTATTTGTTAAA

6579 SEQ ID N0:5016 -5.5 -16.7 54.1 -10.7 -0.1 -3 TTAAAGCCATCTAGAAAATG

7048 SEQ ID N0:5017 -5.5 -16.4 51.8 -10.9 0 -6.2 TTCAGATAGAAACAAACATA

7090 SEQ ID N0:5018 -5.5 -14.8 49 -9.3 0 -3.4 TAACATAGGGTATTTAATTG

7367 SEQ ID N0:5019 -5.5 -16.2 52.6 -10.7 0 -2.9 ACATATTTTTAGCAAAATAT

7808 SEQ TD N0:5020 -5.5 -14.8 49.2 -6.8 -2.5 -7.8 CATTATTTATATTTAAACAA

7835 SEQ ID N0:5021 -5.5 -12.8 45.2 -7.3 0.2 -4.9 AAGTTGATTATATTTTTACA

7853 SEQ ID N0:5022 -5.5 -15.7 52.1 -10.2 0 -2.6 TAGTGCAGCAGGAATTAAAA

8217 SEQ ID N0:5023 -5.5 -18.7 57.1 -13.2 0 -6.6 TAATAGCTTTAGTGCAGCAG

8226 SEQ ID N0:5024 -5.5 -21.5 65.4 -15.1 -0.7 -6.6 TCCCTGCATGCAGTTCTTCA

8392 SEQ ID N0:5025 -5.5 -28.1 80.1 -19.5 -0.2 -14.3 TTATCAAACCTGAAAGTTGG

8714 SEQ ID N0:5026 -5.5 -18.3 55.9 -12.1 -0.4 -3.6 ATTCTCAATAACTATATCAT

8916 SEQ ID N0:5027 -5.5 -16.4 53.3 -10.9 0 -2.4 TCCAAGGGTTCTTGAAAAAA

8948 SEQ ID N0:5028 -5.5 -18.6 56 -11 -2.1 -5.7 ATATGCATTACAACTGGAGT

9009 SEQ ID N0:5029 -5.5 -20.3 61.4 -14.8 0 -6.8 CACCTTATATAATTTATTAT

9074 SEQ ID N0:5030 -5.5 -16 52 -9.8 -0.4 -5.3 TCTTCCTCTGGCAGCTGTTC

134 SEQ ID NO:5031 -5.4 -28.1 83.2 -22 0 -8.9 GCCCAGCATCCAAGATGGTT

167 SEQ ID N0:5032 -5.4 -28.5 77.6 -21.6 -1.4 -6.2 GCTTCAGTTTTCTTGCTCCA

231 SEQ ID N0:5033 -5.4 -26.6 78.8 -21.2 0 -4.4 CTCTTCTGCAGCACGTTTTT

548 SEQ ID N0:5034 -5.4 -25.2 73.6 -19.3 0 -7.7 AAGGCAGAGGTGGCACTGAA

775 SEQ ID N0:5035 -5.4 -24.7 70.6 -18.4 -0.8 -4.7 GAAGAAACGTAAAATCTTCT

1011 SEQ ID N0:5036 -5.4 -15.6 50.2 -8.1 -2.1 -8.6 AGACAGAACACAGTCAGGAT

1225 SEQ ID N0:5037 -5.4 -21.3 64.1 -14.4 -1.4 -4.6 CAGATTGCCCATGAACAGCT

1271 SEQ ID N0:5038 -5.4 -25.4 70.4 -19.5 -0.2 -4.5 AAATGACCAGGACAAAAAAT

1677 SEQ ID N0:5039 -5.4 -15.3 48.6 -9.9 0 -4 GCCACCACAGCCAGGATCAA

1723 SEQ ID N0:5040 -5.4 -29 77.3 -23.6 0 -5.3 CTTCTTCCAAGGTGGCCTGA

1764 SEQ ID N0:5041 -5.4 -27.7 77.7 -21.4 -0.7 -7.2 TGCTGCCGCAACTGCCTGAG

1847 SEQ ID N0:5042 -5.4 -29.5 77.7 -22.8 -1.2 -7.1 CATCATCAGCAAAGTCATTT

2241 SEQ ID N0:5043 -5.4 -20.5 62.2 -15.1 0 -4.l GAATGCTGGCTATGCTCACG

2586 SEQ ID N0:5044 -5.4 -25 70.2 -18.9 -0.4 -5.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GCATGGCGGACATTTCTGTC

2639 SEQ ID N0:5045 -5.4 -26.1 74.5 -18.8 -1.9 -8.8 ATACCAGCATGGCGGACATT

2645 SEQ ID N0:5046 -5.4 -25.7 70.8 -18.3 -2 -7.4 GATGTTTTACTTTTAACCAT

2706 SEQ ID N0:5047 -5.4 -19.1 58.9 -13.7 0 -2.8 CAATCTGCAGATTATTCATT

3471 SEQ ID N0:5048 -5.4 -19.5 60 -11.2 -0.2 -l4 TCCTACTGCAATCTGCAGAT

3479 SEQ ID N0:5049 -5.4 -24.3 70.1 -15.4 -3.5 -12.2 AAGATAATTAAGCTCTTTGC

3644 SEQ ID N0:5050 -5.4 -18 56.6 -11.9 -0.4 -5.7 TGAGAAGCATTTCCAGAATG

4224 SEQ ID N0:5051 -5.4 -20.2 60.5 -13.5 -1.2 -6 AACCAAAGAAACATCAACGA

4307 SEQ ID N0:5052 -5.4 -16.9 51.3 -11.5 0 -3.5 ATGGCACCGAGTTCTGAGTA

4351 SEQ ID N0:5053 -5.4 -25.4 72.8 -20 0 -4 TTGATGGCACCGAGTTCTGA

4354 SEQ ID N0:5054 -5.4 -25.2 71.3 -19.8 0 -4 CATTCATGATAGAGGGAATT

4464 SEQ ID N0:5055 -5.4 -19.2 59 -13.3 -0.1 -6.4 GCCAGCAAACAAATTCACAC

4529 SEQ ID N0:5056 -5.4 -21.4 61.2 -16 0 -4.1 TAATATTTTTTCTGTTCCTC

4930 SEQ ID N0:5057 -5.4 -19.5 61.4 -14.1 0 -4.2 CGTTTCCACCATCATGGTGA

5093 SEQ ID NO:5058 -5.4 -26.3 73.1 -17.2 -3.7 -ll.l CTCCAGTGAACAGAACAATG

5166 SEQ ID N0:5059 -5.4 -20.1 59.5 -14.2 -0.1 -3.9 GGGTGAATTGTGTCAGGGTC

5650 SEQ ID N0:5060 -5.4 -25.1 75.2 -19.7 0 -3.3 AACCAGGAAGGATATGATGA

5735 SEQ ID N0:5061 -5.4 -20 59.3 -13.8 -0.6 -4 AACTCTATAAACTGGGTCGC

5881 SEQ ID N0:5062 -5.4 -21.4 62.8 -16 0 -2.7 GCTGCAAAATCAGAGAGTTT

5905 SEQ ID N0:5063 -5.4 -20.8 62.5 -14.7 -0.4 -4.9 TCTTTGTTATAGTTACTTGA

6241 SEQ ID N0:5064 -5.4 -19 61 -13.6 0 -2.5 TGGAGTTCCCATTTAGTTTG

6312 SEQ ID N0:5065 -5.4 -23.5 69.8 -17.2 -0.8 -4.4 AACTGTAGCCATTACAATTC

7017 SEQ ID N0:5066 -5.4 -20.3 61.1 -13.9 -0.9 -3.9 AAATACTTTCAAAATTAAAG

7062 SEQ ID N0:5067 -5.4 -ll 41.5 -5.6 0.4 -4.4 CAGAAGATGGCAAAGCAATA

7321 SEQ ID N0:5068 -5.4 -19 56.9 -12.7 -0.7 -4.3 ATATATATTTGCCCAAACAT

7415 SEQ ID N0:5069 -5.4 -19.3 57.5 -13.9 0 -5.4 CTTGCAAAAGACACATACAC

7478 SEQ ID N0:5070 -5.4 -18.6 56.3 -11.7 -l.4 -6.9 GAATGGATTGAGTAAAATAG

7583 SEQ ID N0:5071 -5.4 -14.9 49.3 -9.5 0 -2.3 TATTTTTAGCAAAATATGAA

7805 SEQ ID N0:5072 -5.4 -13.8 47.1 -6.8 -1.6 -6 CTATGAATAAAAGGTTATAT

8142 SEQ ID N0:5073 -5.4 -14 47.6 -8 -0.3 -3.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAAGAAGATGAGTTTAGATG

8792 SEQ ID N0:5074 -5.4 -15.4 50.9 -10 0 -1.9 ATGTAACATTTATTAGCCAC

8844 SEQ ID N0:5075 -5.4 -19.5 59.6 -14.1 0 -3.6 CTATATCATTAATACCTTAT

8905 SEQ ID N0:5076 -5.4 -17.1 54.5 -11.7 0 -4.2 CATAAATCCAAGGGTTCTTG

8954 SEQ ID N0:5077 -5.4 -20.5 61 -13.4 -1.7 -5.1 ATTGTCTTTAAAAATATGCA

9022 SEQ ID N0:5078 -5.4 -15.9 51.5 -10.5 0 -5.2 AGAATCCTCTCTGCTTATGC

13 SEQ ID N0:5079 -5.3 -24.1 71.2 -18.1 -0.4 -4.2 AAATTAGTTACAATTTTTTT

74 SEQ ID N0:5080 -5.3 -14.2 48.5 -8.3 -0.3 -3.7 CCCAGCATCCAAGATGGTTA

166 SEQ ID N0:5081 -5.3 -26.4 72.9 -20.2 -0.8 -5.7 CTTATGAATTACAGCATAAC

190 SEQ ID N0:5082 -5.3 -17 53.9 -10.6 -1 -5.1 TTTTCGATAGCAGCAAGAGA

532 SEQ ID N0:5083 -5.3 -21.3 63.5 -16 0 -5.4 AAATGGAAGGTTCTTTCCAG

641 SEQ ID N0:5084 -5.3 -20.8 62.2 -12.4 -3.1 -9.4 CAGCTGCAGCCCAATGAGAG

1256 SEQ ID N0:5085 -5.3 -26.9 74.1 -19.1 0 -13.2 TCATCTCCAATGTAATCCTT

1417 SEQ ID N0:508& -5.3 -22.4 65.9 -16.5 -0.3 -3 TCTGCTCCTCATAGGCCATG

1743 SEQ ID N0:5087 -5.3 -27.8 78.6 -21.8 -0.5 -8.3 TCTTCCAAGGTGGCCTGATT

1762 SEQ ID N0:5088 -5.3 -26.8 75.7 -20.6 -0.7 -7.2 AGCTGATGCTGCCGCAACTG

1853 SEQ TD N0:5089 -5.3 -27.5 74.4 -20.6 -1.6 -8 TCACTGGTCAGTCTGTTTCC

2098 SEQ ID N0:5090 -5.3 -26 78.8 -20.7 0 -6.6 ATACTCAAGAGAGACTGATG

2137 SEQ ID N0:5091 -5.3 -18.7 58.4 -11.8 -1.5 -5.4 TCAGTGGTGGTGCCCTCTGG

2485 SEQ ID N0:5092 -5.3 -30.3 85.9 -23.4 -1.5 -5.8 TTTCAGTGGTGGTGCCCTCT

2487 SEQ ID N0:5093 -5.3 -29.3 84.2 -23.4 -0.3 -5.l ACATGGTCTCTATCCACTCT

3297 SEQ ID N0:5094 -5.3 -25.1 74.4 -18.9 -0.7 -5.2 GAAAGAGGTTCAGAACCACA

3390 SEQ ID N0:5095 -5.3 -21.1 62 -13.1 -2.7 -12 ATGACATATAATCATTTTCA

3729 SEQ ID N0:5096 -5.3 -16.7 53.8 -10.8 -0.3 -5.1 TCTCCAACAGCAATTGGCAC

3778 SEQ TD N0:5097 -5.3 -24.4 69.2 -17.5 -1.5 -8.1 GAAGGATCATGAACACAATG

4104 SEQ ID N0:5098 -5.3 -17.8 54.8 -12.5 0 -6.9 AATTGCTCCAACAAGAGCAT

4448 SEQ ID N0:5099 -5.3 -21.8 63 -13.1 -3.4 -9.5 CCAAAGATGATAAAGATGAC

4816 SEQ ID N0:5100 -5.3 -16.2 51.2 -10.9 0 -2.1 ATCCAAGTTTCTTCATTGCA

4953 SEQ ID N0:5101 -5.3 -22.9 68.2 -17.6 0 -4.8 ATGCTGATATCAAAGACTTG

5047 SEQ ID N0:5102 -5.3 -18.5 57.2 -12.7 0 -7.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ACAAAACTAGGGTCATGTAT

5127 SEQ ID N0:5103 -5.3 -19.2 58.7 -13.4 -0.1 -4.7 GCACAAATTCTCCAGTGAAC

5175 SEQ ID N0:5104 -5.3 -21.8 63.7 -15.7 -0.6 -4.6 GTTCCAGCCTATAGTGAAGT

5222 SEQ ID N0:5105 -5.3 -24.8 72.9 -19.5 0 -5.1 ATACTTTTCTATCATCTCAG

5291 SEQ ID N0:5106 -5.3 -19.6 62.3 -14.3 0 -1.6 GACACAGAATACTTTTCTAT

5299 SEQ ID N0:5107 -5.3 -18.4 57.7 -12.2 -0.7 -4.4 AACGCAGGAAGGGACATCAT

5416 SEQ ID N0:5108 -5.3 -22.9 64.9 -17.6 0 -3.6 ATCCAGGGCAGCTGCAAAAT

5915 SEQ ID N0:5109 -5.3 -25.1 69.7 -16.9 -1 -14 AGTGGAGTTCCCATTTAGTT

6314 SEQ TD N0:5110 -5.3 -24.6 73.4 -18 -1.2 -6.4 ATAGGCTGTAAACAATTGAT

6500 SEQ ID N0:5ll1 -5.3 -18 55.8 -12.7 0 -7.2 CAACTATCCCTATAGTCTAG

6727 SEQ ID N0:5112 -5.3 -21.7 64.9 -14.4 -2 -7.4 GGATGCAAGGACTGTACTAA

6781 SEQ TD N0:5113 -5.3 -21.5 63.6 -16.2 0 -5.6 CTATGAATTGGGGACCATGG

6859 SEQ ID N0:5114 -5.3 -22.9 65.3 -15.2 -2.4 -8.3 AAACCTCATTTACAAAAATA

6901 SEQ TD N0:5115 -5.3 -14.5 47.8 -8.5 -0.5 -3 TGGCAAAACACCTTTGTCTG

6964 SEQ ID N0:5116 -5.3 -22.3 64.2 -14.7 -2.3 -6.6 TTCTTTTTCTGGTTTTGAGC

7000 SEQ ID N0:5117 -5.3 -22.2 69.1 -16.9 0 -2.8 CTGAAACTGTAGCCATTACA

7021 SEQ ID N0:5118 -5.3 -21.3 62.5 -15.1 -0.8 -3.7 TTACAGGCACATAACTGTTC

7107 SEQ TD N0:5119 -5.3 -21.1 63.8 -14.2 -1.5 -5.2 AGCAGAAGATGGCAAAGCAA

7323 SEQ ID N0:5120 -5.3 -21.1 61.4 -14.9 -0.8 -5.6 CATAACATAGGGTATTTAAT

7369 SEQ ID N0:5121 -5.3 -16.8 53.6 -11.5 0 -2.9 TTGCAAAAGACACATACACT

7477 SEQ ID N0:5122 -5.3 -18.6 56.3 -13.3 0 -6.6 AAATAATTTTCTTATGTACA

7888 SEQ ID N0:5123 -5.3 -14.8 49.7 -9.5 0 -6.4 AAAGTAAAATAAATTGTGAT

7921 SEQ ID N0:5124 -5.3 -11.6 42.6 -6.3 0 -3.2 TAATTGCACAACTTTGCCAC

8287 SEQ ID N0:5125 -5.3 -21.8 63.1 -16 -0.2 -5 TTTGGCTCAATAATGACGTA

8440 SEQ ID N0:5126 -5.3 -20 59.7 -14 -0.5 -5.3 AATTGAAAGCTATTGTAGGT

8748 SEQ ID N0:5127 -5.3 -18.4 57.4 -13.1 0 -5.1 TCTCTGCTTATGCCTCCGCT

6 SEQ ID N0:5128 -5.2 -29.7 81.9 -23.6 -0.8 -4.3 AATTAGTTACAATTTTTTTT

73 SEQ ID N0:5129 -5.2 -15 50.6 -9.8 0 -2.6 ' CCTTATGAATTACAGCATAA

191 SEQ ID N0:5130 -5.2 -18.8 57.1 -12.7 -0.8 -4.9 CTTGCTCCATAATCTCTGAT

220 SEQ ID N0:5131 -5.2 -23 67.7 -17.8 0 -3.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGCACATTTAATTACGTGTA

442 SEQ ID N0:5132 -5.2 -19.5 59.2 -13.7 -0.3 -5.4 TTCGATAGCAGCAAGAGATT

530 SEQ ID N0:5133 -5.2 -21.2 63.1 -16 0 -5.4 TTTCGATAGCAGCAAGAGAT

531 SEQ ID N0:5134 -5.2 -21.2 63.1 -16 0 -5.4 GAGGAATGTCTCCATAAATA

660 SEQ ID N0:5135 -5.2 -19.3 58.6 -12.3 -1.8 -4.8 TTTTCCTAACAGGGTTTAGT

810 SEQ ID N0:5136 -5.2 -22.2 67.2 -16.1 -0.7 -4.1 ACCGACTGGATCAGGGCCCC

1183 SEQ ID N0:5137 -5.2 -31.8 82 -24.9 -0.8 -11.5 AGAAAGCTTCTTTACCGACT

1196 SEQ ID N0:5138 -5.2 -21.9 63.7 -15.8 -0.7 -7 GTTAAATGTGCTCATTGTTA

1394 SEQ ID N0:5139 -5.2 -19.7 61.1 -12.9 -1.6 -4 CATGAGTCGAAATAGAGACA

1592 SEQ ID N0:5140 -5.2 -18.7 57 -12.7 -0.6 -5.8 TCAACTGGTAAAGATTTTCC

1626 SEQ ID N0:5141 -5.2 -19.5 59.4 -14.3 0 -3.3 CTGCTTCTTTTGACGCTGTC

2059 SEQ ID N0:5142 -5.2 -25.2 73.6 -18 -2 -6.6 AGCAGAATTTTTTGTCACTG

2112 SEQ ID N0:5143 -5.2 -20.3 62.5 -15.1 0 -4.1 GAACACATTGGCAAATCTAT

2663 SEQ ID N0:5144 -5.2 -19.1 57.6 -13.9 0 -4.4 ATCAAAGATATTCCAGCCTT

2936 SEQ ID N0:5145 -5.2 -22 64.2 -16.1 -0.4 -3.8 AATGATCTTAATTAGCATAT

3077 SEQ ID N0:5146 -5.2 -16.3 52.8 -11.1 0 -4.9 TGCATGCCGACCACAGCAAA

3157 SEQ ID N0:5147 -5.2 -26.6 70.2 -20.3 -1 -9.1 CCTACTGCAATCTGCAGATT

3478 SEQ ID N0:5148 -5.2 -24 68.9 -14.4 -4.4 -13.6 TACACCACTGGTGGTTCCAT

3674 SEQ ID NO:5149 -5.2 -26.6 75 -18.8 -2.6 -12 CCTTCAGTAAAACAAGCTTC

3967 SEQ ID N0:5150 -5.2 -20.3 60.7 -15.1 0 -6.4 GAGAAGGATCATGAACACAA

4106 SEQ ID N0:5151 -5.2 -18.4 56.2 -12.5 -0.5 -6.9 GATGGCACCGAGTTCTGAGT

4352 5EQ ID N0:5152 -5.2 -26.3 74.8 -21.1 0 -4 AACAAGAGCATTCACAACCA

4439 SEQ ID N0:5153 -5.2 -20.9 60.8 -15.7 0.5 -4.1 AGCAAACAAATTCACACCCA

4526 SEQ ID N0:5154 -5.2 -21.6 61 -16.4 0 -4.1 GGATCCAAGTTTCTTCATTG

4955 SEQ ID N0:5155 -5.2 -22.2 66.7 -16.5 0 -8.3 TCATGATGCTGATATCAAAG

5052 SEQ ID N0:5156 -5.2 -18.4 57 -12.3 -0.8 -7.7 CATCCGTTTCCACCATCATG

5097 SEQ ID N0:5157 -5.2 -26.4 72.4 -21.2 0 -4.1 CCCTGGTCATCCGTTTCCAC

5104 SEQ ID N0:5158 -5.2 -30.3 81.4 -23.9 -1.1 -4.5 GAAGGGACATCATCAAAGCA

5409 SEQ ID N0:5159 -5.2 -21.1 62.1 -15.9 0.5 -3.6 CATAGGCAAAGTTGGACATC

5490 SEQ ID N0:5160 -5.2 -21.1 63 -15.9 0 -4.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TATCAAGACAGTGGATCCGG

6006 SEQ TD N0:5161 -5.2 -23 66.2 -17.1 0 -8.9 GCTTTGTAAAGGCAAATAAA

6027 SEQ ID N0:5162 -5.2 -17 53 -10.2 -1.6 -5.9 CATCTGAAATTACGCTGAAT

6190 SEQ ID N0:5163 -5.2 -18.6 56 -13.4 0 -3.3 TCATGTTGAATTAAAAGTGC

7717 SEQ ID N0:5164 -5.2 -17 54 -11.8 0 -4.7 ATATTTTTAGCAAAATATGA

7806 SEQ ID N0:5165 -5.2 -14.5 48.7 -6.8 -2.5 -7.8 CTAAAAAGAACAAAGTCCAT

8256 SEQ ID N0:5166 -5.2 -15.5 49.5 -10.3 0 -2.8 TCATGGCTAAAAAGAACAAA

8262 SEQ ID N0:5167 -5.2 -15.3 49.2 -10.1 0 -4.7 TGGCTCAATAATGACGTAGC

8438 5EQ ID NO:5168 -5.2 -21.6 63.2 -15.7 -0.5 -5.6 ATGCATTTAAGTAACAAAGA

8864 SEQ ID NO:5169 -5.2 -16 51.5 -10.8 0 -6.4 GGAGTTTTCCACTGAGAATA

8994 SEQ ID N0:5170 -5.2 -21.7 65 -15.1 -1.3 -4.8 TTTTTTTTGACCACAGAGGT

98 SEQ ID NO:5171 -5.1 -22.1 66.2 -15.6 -1.3 -4.7 TTCTTGCTCCATAATCTCTG

222 SEQ TD NO:5172 -5.1 -22.9 68.4 -17.8 0 -2.9 ATGAATCCTTGACAGGTATC

280 SEQ ID N0:5173 -5.1 -21.3 64.1 -15.2 -0.9 -3.5 GGAAAAGCTCCAGGTCCCTT

376 SEQ ID N0:5174 -5.1 -27.3 74.7 -21.1 -1 -6.6 TTTCCAGCTTCCAAGTCACT

628 SEQ ID N0:5175 -5.1 -25.9 74.7 -20.8 0 -4.3 TCTGGAGGAATGTCTCCATA

664 SEQ ID N0:5176 -5.1 -23.5 69.3 -15.1 -3.3 -8.5 ATCTCTGGAGGAATGTCTCC

667 SEQ ID N0:5177 -5.1 -24.4 72.5 -17.2 -2.1 -7.4 GCAGAGGTGGCACTGAATCG

772 SEQ ID N0:5178 -5.l -25.4 71.7 -19.4 -0.7 -4.5 CTGTCACATATGCCATCACA

1065 SEQ ID N0:5179 -5.1 -24.3 70 -19.2 0 -6.5 TCATTGTTACATTAACAAAT

1383 SEQ ID N0:5180 -5.l -15.7 51.1 -7.8 -2.8 -8.5 ATCCTTCCAGTTAAATGTGC

1403 SEQ ID NO:5181 -5.1 -23.1 67.6 -18 0 -5.6 TTCACACAGATGTATCCTTC

1513 SEQ ID N0:5182 -5.1 -22.3 67.4 -16.5 -0.5 -3.6 TCATGAGTCGAAATAGAGAC

1593 SEQ ID N0:5183 -5.1 -18.4 57 -12.7 -0.3 -6.9 TGCTCCTCATAGGCCATGGC

1741 SEQ ID N0:5184 -5.1 -29.5 81.9 -22.2 -0.5 -12.5 ATCTATACCAGCATGGCGGA

2649 SEQ ID N0:5185 -5.1 -25.7 71.7 -18.6 -2 -7.4 AGAACCATTTCTGCTGTGAA

2878 SEQ ID N0:5186 -5.l -22.1 64.8 -16.1 -0.8 -5.5 TGTTTATGAATGACATATAA

3738 SEQ ID N0:5187 -5.1 -15.2 50.2 -9 -1 -5.8 GTACTTACTTGACAGAATGG

4003 SEQ ID N0:5188 -5.1 -19.6 60.3 -13.8 -0.4 -4.5 TGTAGCAGGTTTTTCGAAGA

4053 SEQ ID N0:5189 -5.1 -21.9 65.7 -16.3 -0.2 -6.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GGTAAAGACTTTGTCAGCAT

4196 SEQ ID N0:5190 -5.1 -21.4 64.6 -15 -1.2 -5.3 AGGTAAAGACTTTGTCAGCA

4197 SEQ ID N0:5191 -5.1 -21.4 64.9 -15 -1.2 -5.3 CGATCAAGAAATCTAGCCAG

4290 SEQ ID N0:5192 -5.1 -20.5 59.9 -15.4 0 -4.9 TTAAAGCTCTTAATGTCCGT

4380 SEQ ID N0:5193 -5.1 -21.1 62.4 -16 0 -5.6 GTCTTAAAGCTCTTAATGTC

4383 SEQ ID N0:5194 -5.1 -19.6 61.5 -14.5 0 -5.9 TATAAATACATGTACAGATT

4792 SEQ ID N0:5195 -5.1 -15.1 50.1 -9.5 0 -8.2 CAGCACAAATTCTCCAGTGA

5177 SEQ ID N0:5196 -5.1 -23 66.7 -17.1 -0.6 -4.6 GGGACACAGAATACTTTTCT

5301 SEQ ID N0:5197 -5.1 -21.1 63.5 -15.1 -0.8 -4.2 CCCCTTTGCTCCTTTGATCA

5372 SEQ TD N0:5198 -5.1 -29 79 -23.9 0 -6.1 GGTTGTAATTTGGAACAAGC

5573 SEQ ID N0:5199 -5.1 -20 60.5 -13.3 -l.6 -7.3 AGAGGTTGTAATTTGGAACA

5576 SEQ ID N0:5200 -5.1 -19.5 60 -13.7 -0.4 -4.7 GGTCACAGTCGGGTGGTGCA

5634 SEQ ID N0:5201 -5.1 -29.3 83.7 -23 -0.7 -10.2 AAACTTTTCCCAAACCTCAT

5852 SEQ ID N0:5202 -5.1 -21.9 62 -16.8 0 -2.4 CATCGGGATCAAACTTTTCC

5862 SEQ ID N0:5203 -5.1 -22.4 64.3 -17.3 0 -5.3 CACAAAACACGCTTTGTAAA

6037 SEQ ID N0:5204 -5.1 -17.8 53.6 -11.2 -1.4 -6.6 TGGAGGGGTTTGATGCCATA

6105 SEQ ID N0:5205 -5.1 -25.6 73 -19.4 -1 -4.6 ATAATTCTTTGTTTCTTTTT

6473 SEQ ID N0:5206 -5.1 -17.7 57.6 -12.6 0 -2.9 TTTGTAAAAATATGGCAGAT

6821 SEQ ID N0:5207 -5.1 -16.3 52 -10.2 -0.9 -4.9 TGAAACTGTAGCCATTACAA

7020 SEQ ID N0:5208 -5.1 -19.7 58.7 -13.6 -0.9 -3.9 AATATGTGACATTCTTTAAC

7616 SEQ ID N0:5209 -5.1 -16.5 53.4 -11.4 0 -3.9 TAAAAGTGCAAAAGTAAAGG

7706 SEQ ID N0:5210 -5.1 -13.9 46.8 -8.8 0 -5.4 ATAAAGTAAAATAAATTGTG

7923 SEQ ID N0:5211 -5.1 -10.7 40.9 -5.6 0 -3.2 GCATAAAGTAAAATAAATTG

7925 SEQ ID N0:5212 -5.1 -12 43.2 -6.9 0 -3.4 AAAACATCTATGAATAAAAG

8149 SEQ ID N0:5213 -5.1 -11.3 42 -6.2 0 -3.8 AGTGCAGCAGGAATTAAAAA

8216 SEQ ID N0:5214 -5.1 -18.3 55.8 -13.2 0 -6.6 GCTAAAAAGAACAAAGTCCA

8257 SEQ ID N0:5215 -5.1 -17.3 53 -12.2 0 -2.8 CATGTTAGGTAATTGCACAA

8296 SEQ ID N0:5216 -5.1 -19.9 60.3 -14.8 0 -5.3 TTCACAAAGCTGCTTTTAAT

8660 SEQ ID N0:5217 -5.1 -19.4 59 -12.9 -0.1 -10.7 TAGATGCTTATAGCCAAGGG

8778 SEQ ID NO:5218 -5.1 -22.7 66.4 -16.9 -0.5 -4.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATACCTTATGTATACATAGG

8894 SEQ ID N0:5219 -5.1 -19 59 -11.9 -0.7 -12.1 ATCCAAGATGGTTAGGGTAT

160 SEQ ID N0:5220 -5 -22.5 66.9 -16.6 -0.8 -4.9 CATAACAAAGCCCAGCATCC

176 SEQ ID N0:5221 -5 -24.6 67.2 -18.7 -0.7 -4.1 AGCCTTATGAATTACAGCAT

193 SEQ ID N0:5222 -5 -21.6 63.7 -15.9 -0.4 -4.1 TCAAGGTCATAAATACACAG

894 SEQ ID N0:5223 -5 -17.7 55.5 -12.7 0 -2 TGTGTACTCTACATTCTTTG

932 SEQ ID N0:5224 -5 -20.7 64.7 -14.8 -0.8 -4.8 ATGTGTACTCTACATTCTTT

933 SEQ ID N0:5225 -5 -20.7 64.8 -14.8 -0.8 -5.4 ATCACGAAGAAACGTAAAAT

1016 SEQ ID N0:5226 -5 -15 48.1 -9.1 -0.8 -6.l TTCAGTGCTCGGAGAACTCT

1126 SEQ ID N0:5227 -5 -24.4 71.4 -18.5 -0.7 -6.4 TGCCCATGAACAGCTGCAGC

1266 SEQ ID N0:5228 -5 -28.3 76.8 -21 -0.2 -12.8 ATTGTTACATTAACAAATGT

1381 SEQ ID N0:5229 -5 -15.8 51.4 -8 -2.8 -8.5 TCCAGTTAAATGTGCTCATT

1398 SEQ ID N0:5230 -5 -21.8 65.1 -15.3 -1.4 -3.7 GGGTTTCGACCAGCCTTCAC

1528 SEQ ID N0:5231 -5 -28.6 79.1 -20.9 -2.7 -6 AGCTTGTGTAGCCATAGTTG

1548 SEQ ID N0:5232 -5 -24.4 73.1 -17.8 -1.5 -4.6 CTGGTAAAGATTTTCCCAAT

1622 SEQ ID N0:5233 -5 -20.9 61.2 -15.9 0 -4.8 TCTTTAGCACCTTTGGAACT

1945 SEQ TD N0:5234 -5 -23.2 68 -18.2 0 -4.l GAAAATGCTTGTTTTGCTAT

2186 SEQ ID N0:5235 -5 -19.2 58.6 -13.3 -0.8 -4.8 AGAATGCTGGCTATGCTCAC

2587 SEQ ID N0:5236 -5 -24.2 70.4 -18.5 -0.4 -5.2 TATACCAGCATGGCGGACAT

2646 SEQ ID N0:5237 -5 -25.3 69.9 -18.3 -2 -7.4 ACCATTTCTGCTGTGAAAAT

2875 SEQ ID N0:5238 -5 -20.8 61.3 -14.9 -0.7 -5 CAACTTGAAAACTCTAAGCA

3035 SEQ ID N0:5239 -5 -17.6 54.2 -12.6 0 -4.1 GCACGCGGAACACAATCAGG

3270 SEQ ID N0:5240 -5 -24.6 66.7 -19.1 0 -7.9 CAGCACGCGGAACACAATCA

3272 5EQ ID N0:5241 -5 -24.1 65.5 -19.1 0 -7 TGAACTCAATAATAAGGCCA

3410 SEQ ID N0:5242 -5 -19.1 57 -13.6 0 -7.7 AATCTGCAGATTATTCATTT

3470 SEQ ID N0:5243 -5 -18.9 59.1 -11.2 -0.2 -13.6 AGCTGGTTGCATTTAATTTC

3864 SEQ ID N0:5244 -5 -21.5 65.5 -14.9 -1.5 -5.1 ATCAACTGTGCTTCCTTCAG

3887 SEQ ID N0:5245 -5 -24 70.8 -19 0.6 -3.6 AGTCTTTCGCTGTTCAATGT

4157 SEQ ID N0:5246 -5 -23.5 70.5 -18.5 0 I -3.1 CTAGCCAGCACCAGGCATTA

4278 SEQ ID N0:5247 -5 -28 77.4 -20.3 -2.7 -7.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AATCTAGCCAGCACCAGGCA

4281 SEQ ID N0:5248 -5 -27.9 76.8 -20.2 -2.7 -7.4 ACCAGGCTAACCAAAGAAAC

4315 SEQ TD N0:5249 -5 -20.7 59.2 -14.3 -1.3 -5 AGTAGCCAAGAGCATTGGCT

4335 SEQ ID N0:5250 -5 -25.8 74.1 -15.6 -5.2 -14.4 TCCGTAATGATTTGATGGCA

4365 SEQ ID N0:5251 -5 -22.5 64.6 -17.5 0 -4 ACAGTGGTAGAACTTGCCAG

4544 SEQ ID N0:5252 -5 -23.8 69.5 -17.5 -1.2 -5.1 AGCCTTTAAATGTGGCCACT

4707 SEQ ID N0:5253 -5 -25.2 70.5 -17.7 -1.8 -13 TACAGGCTGAAGTTTAACAT

4763 SEQ ID N0:5254 -5 -19.7 60 -14.7 0 -4.1 ATACATGTACAGATTTTCTT

4787 SEQ ID N0:5255 -5 -18.7 59.2 -13 0 -9.1 AAATACATGTACAGATTTTC

4789 SEQ ID N0:5256 -5 -16.3 53 -10.8 0 -8.2 AGTATAAATACATGTACAGA

4794 SEQ TD N0:5257 -5 -16.2 52.7 -10.5 -0.5 -8.2 GTCATAAAGATGTCTTGACC

4909 SEQ ID N0:5258 -5 -20.6 62.5 -14.1 -1.4 -7.7 TGTCATAAAGATGTCTTGAC

4910 SEQ ID N0:5259 -5 -18.6 58.5 -12.7 -0.8 -8.3 GGACAAAACTAGGGTCATGT

5129 SEQ ID N0:5260 -5 -21.3 63.1 -15 -1.2 -5 GGGACAAAACTAGGGTCATG

5130 SEQ ID N0:5261 -5 -21.3 62.6 -l5 -1.2 -5 ATGTTCCAGCCTATAGTGAA

5224 SEQ ID N0:5262 -5 -23.6 69 -18.6 0 -5.1 CGGAACAAGGTAGGGGACAC

5314 SEQ ID N0:5263 -5 -23.5 66.3 -17.9 -0.3 -3.2 CCCTTTGCTCCTTTGATCAG

5371 SEQ ID N0:5264 -5 -27 75.9 -22 0 -6.5 AGTTGAACATGTCATCAATT

5526 SEQ ID N0:5265 -5 -18.9 58.8 -12.6 -1 -9.9 GCACTATTAAGAATAGGTGC

5617 SEQ ID N0:5266 -5 -20.2 61.4 -12.6 -2.6 -8.1 AGAGAGTTTAGAGAACTCTA

5894 SEQ ID N0:5267 -5 -18.9 60.3 -9.7 -4.2 -9.6 CAGCTGCAAAATCAGAGAGT

5907 SEQ ID N0:5268 -5 -21.3 63.2 -15.6 -0.4 -7.2 CCAGGGCAGCTGCAAAATCA

5913 SEQ ID N0:5269 -5 -25.8 70.8 -18.3 -1 -13.2 GGCAGACACCTCCTCTTGTT

6164 SEQ ID N0:5270 -5 -28.3 80.2 -23.3 0 -4 AAATTACGCTGAATGATAGC

6184 SEQ ID N0:5271 -5 -18.1 55.2 -12.3 -0.6 -4.4 GTGTCAGCTGGTAATAAAAA

6682 SEQ ID N0:5272 -5 -18.5 57 -12.9 0 -8.6 ACCATGGAAATGCACTAGAA

6846 SEQ ID N0:5273 -5 -20.5 59.7 -14.1 -1.3 -8.8 TCTGGTTTTGAGCAAAAATT

6993 SEQ ID N0:5274 -5 -18.4 56.7 -12.8 -0.3 -7.6 GCAGAAGATGGCAAAGCAAT

7322 SEQ ID N0:5275 -5 -21.1 61.2 -15.2 -0.7 -4.9 GTGTTTGTAAATAGCTTCAA

7687 SEQ ID N0:5276 -5 -19 59.3 -14 0 -4.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AACAAGTTTTAGACATATTT

7820 SEQ ID N0:5277 -5 -16.1 52.6 -11.1 0,4 -3.1 TAAACAAGTTTTAGACATAT

7822 SEQ ID N0:5278 -5 -14.9 49.7 -9.2 -0.4 -5.5 TTGGAATTGTGATTAAAAAT

7956 SEQ ID N0:5279 -5 -14.1 47.4 -9.1 0 -3.1 ATGTTAGGTAATTGCACAAC

8295 SEQ ID N0:5280 -5 -19.4 59.6 -14.4 0 -5.6 ATCATGTTAGGTAATTGCAC

8298 SEQ ID N0:5281 -5 -20.3 62.5 -15.3 0 -5.3 TAAACTGCCAACAGCAAAAA

8521 SEQ ID N0:5282 -5 -17.8 53.2 -11.2 -1.5 -4.5 TAACTATATCATTAATACCT

8908 SEQ ID N0:5283 -5 -16.5 52.9 -11.5 0 -4.2 CTTTCCAGAATCCTCTCTGC

19 SEQ ID N0:5284 -4.9 -25.8 74.6 -20.2 -0.4 -4 TCCGGAAAAGCTCCAGGTCC

379 SEQ ID N0:5285 -4.9 -27.5 73.9 -20.7 -1.9 -7.8 CAACAGTGCCTGTGCCATCT

470 SEQ ID N0:5286 -4.9 -27.8 77.1 -21.3 -1.5 -8.7 AAAGGCGGAAGCTTTCAGGT

501 SEQ ID N0:5287 -4.9 -23.9 68 -16.6 -2.4 -7.6 AATGGAAGGTTCTTTCCAGC

640 SEQ ID N0:5288 -4.9 -23.3 68.5 -15.3 -3.1 -9.4 TAAAATATACAAGGCAGAGG

785 SEQ ID N0:5289 -4.9 -16.7 52.7 -11.8 0 -4 TCAAAATAGTGCACATGATA -870 SEQ ID N0:5290 -4.9 -17.6 54.9 -11.7 0 -9.9 ACTCTGTCACATATGCCATC

1068 SEQ ID N0:5291 -4.9 -24.2 71.3 -19.3 0 -6.5 ATCAGAAAGCTTCTTTACCG

1199 SEQ ID N0:5292 -4.9 -21.3 &2.6 -15.6 -0.6 -7 ATGTGCTCATTGTTACATTA

1389 SEQ ID N0:5293 -4.g -20.8 64.1 -15.4 -0.1 -1.1 AAGCTTGTGTAGCCATAGTT

1549 SEQ ID N0:5294 -4.9 -23.7 70.7 -17.2 -1.5 -7.5 AGGACAAAAAATATCATGTA

1669 SEQ ID N0:5295 -4.9 -14.9 49 -10 0 -4.7 GTCTCTCCTGCTTTCGCCGT

2279 SEQ TD N0:5296 -4.9 -31 85.3 -25.4 -0.5 -3.6 CAAGATGTTTTACTTTTAAC

2709 SEQ ID N0:5297 -4.9 -16.4 53.3 -11.5 0 -2.9 GCTCCATTAAACTGAGGCTG

2967 SEQ ID N0:5298 -4.9 -23.8 68.1 -18.4 -0.1 -4.1 CACAGCAAAAATGAAGACGA

3146 SEQ ID N0:5299 -4.9 -17.4 52.6 -12.5 0 -4.1 ATGTGCCACCGTGGGAGCGT

3229 SEQ ID N0:5300 -4.9 -30.7 81 -24.6 -1.1 -8.8 TTGTCATCATCAGTAGCAGC

3448 SEQ ID N0:5301 -4.9 -23.8 72,9 -18.9 0 -4.1 AAAAAGGCTTTTTGGAAACA

3544 SEQ ID N0:5302 -4.9 -16.8 52.3 -10.8 -1 -7.9 TTCTAAAAAAGGCTTTTTGG

3548 SEQ TD N0:5303 -4.9 -17.1 53.8 -10.7 -1.4 -7.7 AAGCTCTTAATGTCCGTAAT

4377 SEQ ID N0:5304 -4.9 -2l 62.1 -16.1 0 -5 AGTCACTCAAATTGTTAACA

4602 SEQ ID N0:5305 -4.9 -18.4 57.5 -l3 0 -8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGACCCAAAGATGATAAAGA

4820 SEQ ID N0:5306 -4.9 -18.2 54.7 -13.3 0 -1.9 TGTTCCTCTGTCATAAAGAT

4918 SEQ ID N0:5307 -4.9 -21.1 64.1 -16.2 0 -3.5 CTTGGATCCAAGTTTCTTCA

4958 SEQ ID N0:5308 -4.9 -23.1 68.7 -15.1 -0.7 -14.3 GCTCCTTTGATCAGACGTAG

5365 SEQ ID N0:5309 -4.9 -24.4 71 -19.5 0 -6.5 AGGGACATCATCAAAGCAAA

5407 SEQ ID N0:5310 -4.9 -19.8 58.9 -14.3 -0.3 -4.4 CCAAAGATGGCATAGATAAA

5470 SEQ ID N0:5311 -4.9 -18.2 54.9 -12.4 -0.8 -5 CAAAGTCATCCTCACTCAGG

5823 SEQ ID N0:5312 -4.9 -23.4 68.5 -17.8 -0.4 -3.1 AACATCTCAAAGTCATCCTC

5830 SEQ ID N0:5313 -4.9 -21.2 63.8 -16.3 0 -2.8 TTTTCCCAAACCTCATAGAA

5848 SEQ ID N0:5314 -4.9 -21.8 62.5 -16.9 0 -2.8 TCCACTCTCACACAAAACAC

6047 SEQ ID N0:5315 -4.9 -21.4 62.3 -16.5 0 0 TCATAAGAGACTTTGGAGGG

6118 SEQ ID N0:5316 -4.9 -20.8 63.1 -15.9 0 -3.4 AATTGCCTCTTTGTTATAGT

6248 SEQ ID N0:5317 -4.9 -21.5 65.5 -16.6 0 -3 TTGTCAACGTTGATACCCTG

6652 SEQ ID N0:5318 -4.9 -23.4 66.5 -16.2 -0.1 -12.8 TTAAATATTAGAGGAGACTT

7126 SEQ ID N0:5319 -4.9 -16.1 52.6 -10.3 -0.7 -5.9 CTAAATTAAATATAGAGCTC

7206 SEQ ID N0:5320 -4.9 -14.7 49.3 -9.3 0 -8 TAAACTATGTTTAATGAATG

7523 SEQ ID N0:5321 -4.9 -13.4 46.3 -7.6 -0.8 -5.5 ATAGCTTCAAATTATTCTGC

7677 SEQ ID N0:5322 -4.9 -19.2 59.7 -14.3 0 -4.6 CAAGTTTTAGACATATTTTT

7818 SEQ ID N0:5323 -4.9 -16.8 54.6 -11.2 -0.4 -2.9 AACCATAGTTGGTAGAAAAT

8116 SEQ ID N0:5324 -4.9 -17.8 55 -10.5 -2.4 -6.3 CAGTTCTTCAATACACTACA

8382 SEQ ID N0:5325 -4.9 -20.1 61.8 -15.2 0 -3.1 TTCCCTGCATGCAGTTCTTC

8393 SEQ ID N0:5326 -4.9 -27.5 79.4 -19.5 -0.2 -14.3 GGTTTTGACCTCACATAAAT

8967 SEQ ID N0:5327 -4.9 -20.7 61.6 -14:2 -1.6 -4.2 TATATAATTTATTATGGGAG

9069 SEQ ID N0:5328 -4.9 -15.1 50.5 -9.4 -0.6 -4.9 GCAGCTGTTCAGCTTTTTTT

124 SEQ ID N0:5329 -4.8 -24.9 75 -18.2 -1.8 -11 TAACGTGTCTTCCTCTGGCA

141 SEQ ID N0:5330 -4.8 -26.1 74.4 -21.3 0 -5.3 CAATATCCCTAGAAGAGATT

331 SEQ ID N0:5331 -4.8 -19.9 59.6 -15.1 0 -0.4 CTTTTTCCGGAAAAGCTCCA

384 SEQ ID N0:5332 -4.8 -24 66.6 -16.1 -1.6 -14.4 TCCTTTATTCATTACTATAA

740 SEQ ID N0:5333 -4.8 -18 57 -13.2 0 -2.3 GTGGCACTGAATCGGAAAAT

766 SEQ ID N0:5334 -4.8 -20.8 60 -16 0 -4.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AATCTTCTAAGCAAAACCCT

999 SEQ ID N0:5335 -4.8 -20.5 59.7 -15.7 0 -4.1 ACAGCTGCAGCCCAATGAGA

1257 SEQ ID N0:5336 -4.8 -27.1 74.4 -19.8 0 -13.2 TAAATGTGCTCATTGTTACA

1392 SEQ ID N0:5337 -4.8 -19.3 59.5 -12.9 -1.6 -4 TTTTTGCCCATCCAAAACAT

1448 SEQ ID N0:5338 -4.8 -22.8 63.7 -16.5 -1.4 -5.4 GATTCTGCTCCTCATAGGCC

1746 SEQ ID N0:5339 -4.8 -27.8 79.5 -22.3 -0.5 -6.4 CTTTGGAACTCAACTTTGAT

1935 SEQ ID N0:5340 -4.8 -19.6 59.6 -14.2 -0.3 -5 AGAGCCCCCACAGAATTGCC

3097 SEQ TD N0:5341 -4.8 -29.8 78 -25 0 -3.2 GCTGCATGCCGACCACAGCA

3159 SEQ ID N0:5342 -4.8 -30.7 80.3 -23.3 -2.6 -9.7 TCGTTCATGTGCCACCGTGG

3235 SEQ ID N0:5343 -4.8 -28.7 77.5 -23.1 -0.6 -8.4 ATGAACTCAATAATAAGGCC

3411 SEQ ID N0:5344 -4.8 -18.4 55.8 -13.6 0 -6.4 TCATCATCAGTAGCAGCAAG

3445 SEQ ID N0:5345 -4.8 -22.5 68 -17.7 0 -5.4 AACTGTGCTTCCTTCAGATG

3884 SEQ ID NO:5346 -4.8 -23.5 69.2 -17.8 -0.7 -4 AAGGATCATGAACACAATGA

4103 SEQ ID N0:5347 -4.8 -17.8 54.8 -12.5 -0.1 -7.4 TATATATCTTCAAAGGCCAA

4138 SEQ ID N0:5348 -4.8 -19.2 58.2 -13.9 0 -7.7 ATTCATGATAGAGGGAATTG

4463 SEQ ID N0:5349 -4.8 -18.5 57.7 -13.7 0 -6.4 GATGACAAAGTATAAATACA

4802 SEQ ID N0:5350 -4.8 -14.5 48.3 -9 -0.5 -5.8 AGATGACAAAGTATAAATAC

4803 SEQ ID N0:5351 -4.8 -13.8 47.1 -9 0 -4.5 ATGACCCAAAGATGATAAAG

4821 SEQ ID N0:5352 -4.8 -17.6 53.5 -12.8 0 -2.1 TCAAAGACCATTCCTTGGAA

5014 SEQ ID N0:5353 -4.8 -21.8 62.8 -15.4 -1.5 -6.9 TGAATTGTGTCAGGGTCACA

5647 SEQ ID N0:5354 -4.8 -23.1 69.1 -17.1 -1.1 -5.6 CACTTTCTTCAGTAGCAACA

5796 SEQ ID N0:5355 -4.8 -22.1 66.7 -16.6 -0.5 -0.8 TCAAAGTCATCCTCACTCAG

5824 SEQ ID N0:5356 -4.8 -22.6 67.5 -17.8 0 -2.6 AGAAGAGGAGGATCCAGGGC

5926 SEQ ID N0:5357 -4.8 -25.4 73.1 -18 -2.6 -9 GGAGGGGTTTGATGCCATAA

6104 SEQ ID N0:5358 -4.8 -24.9 70.8 -19.4 -0.5 -4 GACTTTGGAGGGGTTTGATG

6110 SEQ ID N0:5359 -4.8 -23.3 69.1 -18.5 0 -2.6 TCTCAAACTTTTCCTTGTCT

6399 SEQ ID N0:5360 -4.8 -22.3 67.1 -17.5 0 -2.8 CTGGTTTGTCTTTCTCAAAC

6411 SEQ ID N0:5361 -4.8 -21.4 65.6 -14.2 -2.4 -7.4 CCTCTTGAAGTCCAGTTGAC

6537 SEQ ID N0:5362 -4.8 -24.6 71.8 -18.9 -0.7 -3.7 AGTATTTGTTAAAACAGTCA

6572 SEQ ID N0:5363 -4.8 -17.2 55.3 -12.4 0 -5.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GAGGTCACTTCACTGTCTTG

6609 SEQ ID N0:5364 -4.8 -24.1 73.3 -19.3 0 -3 ACTTTGCACAACTATCCCTA

6735 SEQ ID N0:5365 -4.8 -23.8 67.9 -19 0 -5 TAAAAATATGGCAGATATGG

6817 SEQ ID N0:5366 -4.8 -15.8 50.7 -9.6 -1.3 -4.7 TCAACGTAAACCTCATTTAC

6908 SEQ ID N0:5367 -4.8 -19.2 57.6 -13.2 -1.1 -5.3 ACTGAAACTGTAGCCATTAC

7022 SEQ ID N0:5368 -4.8 -20.8 61.9 -16 0 -3.3 TAACTGAAACTGTAGCCATT

7024 SEQ ID N0:5369 -4.8 -19.9 59.4 -15.1 0 -3.3 GTATCCAGTGTGTTTCCTTG

7763 5EQ ID N0:5370 -4.8 -25.2 75.3 -20.4 0 -3.2 GCTGACAAATAAAGTTGATT

7864 SEQ ID N0:5371 -4.8 -17.1 53.7 -12.3 0.8 -3.2 AAAAAAACATCTATGAATAA

8152 SEQ ID N0:5372 -4.8 -10.6 40.6 -5.8 0 -3.8 TACGTAAATACTACAAAAGT

8179 SEQ ID N0:5373 -4.8 -14.7 48.4 -9.2 -0.4 -5.8 AGGTAATTGCACAACTTTGC

8290 SEQ ID N0:5374 -4.8 -21.3 63.4 -15.8 -0.5 -5.3 ATAATGACGTAGCTCACCAT

8431 SEQ ID N0:5375 -4.8 -22.2 64.2 -17.4 0 -5.3 AATTATGAAATACATATTTA

9049 SEQ ID N0:5376 -4.8 -12.5 44.6 -6.6 -1 -5.2 AAAGAGACCTTTCCAGAATC

27 SEQ ID N0:5377 -4.7 -20.5 60.9 -14.9 -0.8 -3.7 CAAACCTTGATGTGGCTTGG

250 SEQ ID N0:5378 -4.7 -23.5 66.7 -18.8 0 -3.7 AAGGCGGAAGCTTTCAGGTC

500 SEQ ID N0:5379 -4.7 -25 71.8 -17.9 -2.4 -7.4 TTCCTTTATTCATTACTATA

741 SEQ ID N0:5380 -4.7 -18.8 59.3 -14.1 0 -1.5 TCCACAAACTCTGTCACATA

1075 SEQ ID N0:5381 -4.7 -21.9 64.4 -16.7 -0.1 -4.2 CTGAATGTTCTCAACGCTGA

1108 SEQ ID N0:5382 -4.7 -22.1 64.3 -16.5 -0.8 -4.8 CCTGGAATGACTGAAATTGT

1147 SEQ ID N0:5383 -4.7 -20.2 59.5 -15.5 0 -3.6 AGCTGCAGCCCAATGAGAGC

1255 SEQ TD N0:5384 -4.7 -28 77.3 -20.8 -0.7 -13.2 CAGAATCGCTTGGGGGCCAC

1308 SEQ ID N0:5385 -4.7 -28.1 75.8 -22.7 -0.5 -7.6 TGTGCTCATTGTTACATTAA

1388 SEQ ID NO:5386 -4.7 -20.1 61.9 -15.4 0 -4.1 TTTTGCCCATCCAAAACATA

1447 5EQ ID N0:5387 -4.7 -22.4 62.8 -16.5 -1.1 -5 TCCAAGGTGGCCTGATTCTG

1759 SEQ ID N0:5388 -4.7 -26.7 75.2 -21.1 -0.7 -7.2 CTGCTTCTTCCAAGGTGGCC

1767 SEQ ID N0:5389 -4.7 -28.9 80.8 -23.3 -0.7 -6.3 TTCGGCCTCTTTTTGTTCTG

1784 SEQ ID N0:5390 -4.7 -25.5 74.2 -20.8 0 -6.7 TCTGCTGAAATTCGGCCTCT

1794 SEQ ID N0:5391 -4.7 -25.7 71.7 -19 -2 -10.2 TTCTGAAGAACTTTCCAACA

1913 SEQ ID N0:5392 -4.7 -19.8 59.5 -14.4 -0.4 -4.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Tnter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGTGGTGGTGCCCTCTGGGG

2483 SEQ ID N0:5393 -4.7 -31.6 88.3 -25.1 -1.8 -6.6 TCCGTTTCAGTGGTGGTGCC

2491 SEQ TD N0:5394 -4.7 -29.5 83.3 -24.8 0 -4.7 CCAGGTTTCCTACAGTCAAC

2844 SEQ ID N0:5395 -4.7 -25 72.4 -19.8 -0.1 -3.9 AGACCAGGTTTCCTACAGTC

2847 SEQ ID N0:5396 -4.7 -25.6 75.5 -20.2 -0.5 -5.9 AACCATTTCTGCTGTGAAAA

2876 SEQ ID N0:5397 -4.7 -20.1 59.4 -14.9 -0.2 -4.5 CCACTCTCCACACAGCACGC

3284 SEQ ID N0:5398 -4.7 -29.3 78.6 -24.6 0 -4.1 CATGGTCTCTATCCACTCTC

3296 SEQ ID N0:5399 -4.7 -25.3 75.6 -19.7 -0.7 -3.9 CATACAGTCCCACATGGTCT

3308 SEQ ID N0:5400 -4.7 -26.2 74.7 -21.5 0 -5.2 TCCATACAGTCCCACATGGT

3310 SEQ ID N0:5401 -4.7 -27.3 76.3 -21.4 -1.1 -5.2 CTAAATGAACTCAATAATAA

3415 SEQ ID N0:5402 -4.7 -12.6 44.4 -7.9 0 -3.4 ACACCACTGGTGGTTCCATT

3673 SEQ ID N0:5403 -4.7 -27 76 -19.7 -2.6 -12 AAACCAGTTGTGCTCAACAA

4076 SEQ ID N0:5404 -4.7 -21.3 62 -13.3 -3.3 -6.6 TCATGAACACAATGAAAGTC

4098 SEQ ID N0:5405 -4.7 -16.9 53.3 -11.6 -0.3 -6.9 CTGTTCAATGTATATATCTT

4148 SEQ ID N0:5406 -4.7 -18.3 58.4 -13.6 0 -3.9 CCCATTTGAGAAGCATTTCC

4230 SEQ ID N0:5407 -4.7 -24.5 69 -19 -0.6 -4.1 AGTGAAATATGTTTGAAATC

4259 SEQ TD N0:5408 -4.7 -15.1 50.1 -10.4 0 -4.1 TCATGCCTTCAAACCGGGAT

4416 5EQ ID N0:5409 -4.7 -25.6 69.4 -20.9 0 -7.1 ATACAGGCTGAAGTTTAACA

4764 SEQ ID N0:5410 -4.7 -19.7 60 -15 0 -4.l TCCGTTTCCACCATCATGGT

5095 SEQ TD N0:5411 -4.7 -28.1 77.1 -21.8 -1.5 -6.9 AGCACAAATTCTCCAGTGAA

5176 SEQ ID N0:5412 -4.7 -21.6 63.4 -16.1 -0.6 -4.6 TCGGAACAAGGTAGGGGACA

5315 SEQ ID N0:5413 -4.7 -23.7 67.2 -18.4 -0.3 -3.3 CAGGGTCACAGTCGGGTGGT

5637 SEQ ID N0:5414 -4.7 -28.7 82.3 -23.2 -0.6 -5.6 AAAATCCCAACAGATGGGTC

5698 SEQ ID N0:5415 -4.7 -21.8 62.4 -14.5 -2.6 -7.5 GTTTGATGCCATAAACCTGT

6098 SEQ ID N0:5416 -4.7 -23.1 66.1 -17.9 -0.1 -4.2 AGAGAGGTCACTTCACTGTC

6612 SEQ ID N0:5417 -4.7 -23.7 73.1 -18.5 -0.2 -4.1 TGCAAGGACTGTACTAAAGG

6778 SEQ ID N0:5418 -4.7 -20.2 60.5 -15.5 0 -4.8 ATGAATTGGGGACCATGGAA

6857 SEQ ID N0:5419 -4.7 -22.2 63.3 -15.1 -2.4 -8.8 TATGAATTGGGGACCATGGA

6858 SEQ ID N0:5420 -4.7 -22.6 64.7 -15.5 -2.4 -8.8 TTCTGGTTTTGAGCAAAAAT

6994 SEQ ID N0:5421 -4.7 -18.4 56.7 -12.8 -0.8 -8.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTTTCTGGTTTTGAGCAAAA

6996 SEQ ID N0:5422 -4.7 -19.3 59.3 -14.1 -0.1 -7.9 TACAGGCACATAACTGTTCA

7106 SEQ ID N0:5423 -4.7 -21.7 64.7 -15.4 -1.5 -5.2 TACTCATGTTGAATTAAAAG , 7720 SEQ ID N0:5424 -4.7 -14.8 49.3 -10.1 0 -4.7 GCACAACTTTGCCACTTTGT

8282 SEQ ID N0:5425 -4.7 -25 71.1 -19.8 -0.2 -3.8 AATTAACATTAAACTTTTGG

8342 SEQ ID N0:5426 -4.7 -14.3 48.1 -9.6 0 -3.5 ATGACGTAGCTCACCATTCT

8428 SEQ ID N0:5427 -4.7 -24.6 70.7 -19.9 0 -5.3 ATATAAATTCTCAATAACTA

8922 SEQ ID N0:5428 -4.7 -13.6 46.9 -8.9 0 -2.6 CTTTCCCAGTAAGCCACTCT

303 SEQ ID N0:5429 -4.6 -27.3 76.5 -22.7 0 -3.4 ACCATCTCTGGAGGAATGTC

670 SEQ ID N0:5430 -4.6 -24 70.5 -17.8 -l.5 -6 GAGAACTCTGAATGTTCTCA

1115 SEQ ID N0:5431 -4.6 -20.5 62.8 -11.8 -4.1 -12 TGAAATTGTTTTCAGTGCTC

1136 SEQ ID N0:5432 -4.6 -20.2 62.5 -13.5 -2.1 -7.7 CAGAGTAAAGGGTCTTTTTG

1462 SEQ ID N0:5433 -4.6 -20.1 62.1 -14.8 -0.4 -4.1 CTTGTGTAGCCATAGTTGGG

1546 SEQ ID N0:5434 -4.6 -25 73.7 -18.9 -1.4 -4.3 TGAAATTCGGCCTCTTTTTG

1789 SEQ ID N0:5435 -4.6 -22.1 63.9 -17.5 0 -6.7 CCATTCTTTAGCACCTTTGG

1949 SEQ ID N0:5436 -4.6 -25 71.6 -20.4 0 -4.1 AGAGAGACTGATGAGGGGAG

2130 SEQ ID N0:5437 -4.6 -22.3 66.9 -17.7 0 -2.l GCACAAACAGTGAGTCTCTC

2292 SEQ ID N0:5438 -4.6 -22.9 68.9 -16.7 -1.5 -5.2 TTCAGTGGTGGTGCCCTCTG

2486 SEQ ID N0:5439 -4.6 -29.2 83.5 -23.4 -1.1 -5.6 AAAGAGGGTATTTAAGACAA

2780 SEQ ID N0:5440 -4.6 -16.5 52.7 -11.9 0 -2.9 ATTTAGTGTGGGCCAGGATT

3059 SEQ ID N0:5441 -4.6 -25.1 73.3 -20 0 -7.6 ATCTTAATTAGCATATTTAG

3073 SEQ ID N0:5442 -4.6 -16.3 53.6 -11.7 0 -4.1 CCCCCACAGAATTGCCAATG

3093 SEQ ID N0:5443 -4.6 -27.4 71.1 -22.8 0 -4.3 AAACAATAAGGCACATGGTT

3342 SEQ TD N0:5444 -4.6 -18.9 56.8 -14.3 0 -5.2 AGAAAGAGGTTCAGAACCAC

3391 SEQ ID N0:5445 -4.6 -20.4 61.1 -13.1 -2.7 -12 ACTGTGCTTCCTTCAGATGA

3883 SEQ ID N0:5446 -4.6 -24.8 73 -19.3 -0.7 -4.2 CTTCCGGTTTAAAGTCTTCT

3951 SEQ ID N0:5447 -4.6 -23 67.7 -18.4 0 -6.6 AGAAGGATCATGAACACAAT

4105 SEQ ID N0:5448 -4.6 -17.8 55 -12.5 -0.5 -6.9 TCAATGTATATATCTTCAAA

4144 SEQ ID N0:5449 -4.6 -15.8 51.9 -11.2 0 -3.9 CATTTGAGAAGCATTTCCAG

4228 SEQ'ID N0:5450 -4.6 -21.2 63.2 -15.8 -0.6 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GAAATCCATAAGCAACCCAT

4245 SEQ ID N0:5451 -4.6 -22 61.5 -17.4 0 -4.1 TTGAAATCCATAAGCAACCC

4247 SEQ ID N0:5452 -4.6 -21.4 60.7 -16.8 0 -4.1 TTTGAAATCCATAAGCAACC

424$ SEQ ID N0:5453 -4.6 -19.5 57.5 -14.9 0 -4.1 GGCACCGAGTTCTGAGTAGC

4349 SEQ ID N0:5454 -4.6 -27.2 77.8 -22.6 0 -4 CTCTTAATGTCCGTAATGAT

4374 SEQ ID N0:5455 -4.6 -20.5 61.1 -15.9 0 -2.6 AACCGGGATAAGGCTCTTAG

4405 5EQ ID N0:5456 -4.6 -23.3 66.2 -18.2 -0.2 -7.4 GACCCAAAGATGATAAAGAT

4819 SEQ ID N0:5457 -4.6 -18.2 54.7 -13.6 0 -2.1 CAAGTTTCTTCATTGCATTG

4950 SEQ ID N0:5458 -4.6 -20.6 63.1 -16 0 -5.6 ACATCATCAAAGCAAAGAGC

5403 SEQ ID N0:5459 -4.6 -19.2 58.1 -13.9 -0.5 -4.1 AAGGGACATCATCAAAGCAA

5408 SEQ ID N0:5460 -4.6 -19.8 58.9 -14.6 -0.3 -4.4 CCAGGATGACCGCGATGTAC

5766 SEQ ID N0:5461 -4.6 -26.4 70.8 -20.4 -1.3 -7.2 TCCAGGATGACCGCGATGTA

5767 SEQ ID N0:5462 -4.6 -26.6 71.8 -20.6 -1.3 -7.2 GCAGCTGCAAAATCAGAGAG

5908 SEQ ID N0:5463 -4.6 -21.9 64.2 -14.7 -0.4 -13.4 TCCAGGGCAGCTGCAAAATC

5914 SEQ ID N0:5464 -4.6 -25.5 71.2 -18 -1 -14 CACGCTTTGTAAAGGCAAAT

6030 SEQ ID N0:5465 -4.6 -20.4 59.2 -13.8 -2 -7.2 ATCTCTCCACTCTCACACAA

6052 SEQ ID N0:5466 -4.6 -24.3 71.1 -19.7 0 -0.3 CTCTTTGTTATAGTTACTTG

6242 SEQ ID N0:5467 -4.6 -19.3 61.7 -14.7 0 -2.5 TTTTGTTACACTATCATAGG

6377 SEQ ID N0:5468 -4.6 -19 60 -14.4 0 -3.3 GGGACCATGGAAATGCACTA

6849 SEQ ID N0:5469 -4.6 -23.6 66.2 -18.1 -0.8 -8.8 TGTTTCTTCAACGTAAACCT

6915 SEQ ID N0:5470 -4.6 -20.8 61.4 -15.1 -1 -6.6 CATCTAGAAAATGGAAGTAA

7041 SEQ ID N0:5471 -4.6 -15.5 50.4 -10.9 0 -6.2 GTTTAATGAATGATACAGGA

7515 SEQ ID N0:5472 -4.6 -17.3 54.7 -12.7 0 -2.5 AAAGTGCAAAAGTAAAGGTG

7704 SEQ ID N0:5473 -4.6 -16.1 51.3 -11.5 0 -5.4 AAATTGTGATGTCATCAACC

7911 SEQ ID N0:5474 -4.6 -19.4 58.8 -13.7 -1 -7.2 CAGTAAATAGTTTTGTAAAA

8364 SEQ ID N0:5475 -4.6 -14.2 48.2 -9.6 0 -4.1 ATGCAGTTCTTCAATACACT

8385 SEQ ID N0:5476 -4.6 -21.3 64.7 -16.7 0 -5.5 AAACTGCCAACAGCAAAAAA

8520 5EQ ID N0:5477 -4.6 -17.4 52.2 -11.2 -1.5 -4.5 TTAGATGCTTATAGCCAAGG

8779 SEQ ID N0:5478 -4.6 -21.6 64.2 -15.7 -1.2 -4.9 AATAAGAGTTTGGTTTTGAC

8978 SEQ ID N0:5479 -4.6 -17.8 56.8 -13.2 0 -2.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TACAACTGGAGTTTTCCACT

9001 SEQ ID N0:5480 -4.6 -22.5 66.4 -15.8 -2.1 -5.6 AATATGCATTACAACTGGAG

9010 SEQ ID N0:5481 -4.6 -18.4 56.5 -13.8 0 -6.8 GAATTATGAAATACATATTT

9050 SEQ 2D N0:5482 -4.6 -13.4 46.3 -7.7 -l -5.l AACGTGTCTTCCTCTGGCAG

140 SEQ ID N0:5483 -4.5 -26.4 75.3 -21.9 0 -5.3 TGCTCCATAATCTCTGATAA

218 SEQ ID N0:5484 -4.5 -2l 62.7 -16.5 0 -3.6 AGTCACAGCACCTTTTTCCG

395 SEQ ID N0:5485 -4.5 -26.4 74.4 -21.9 0 -4.1 TTTCAGGTCCTGGGGGTACC

489 SEQ ID N0:5486 -4.5 -28.9 82.4 -21.1 -3.3 -12.6 GGTGGCACTGAATCGGAAAA

767 SEQ ID N0:5487 -4.5 -22 62.3 -17.5 0 -4.5 CCAGTGAATGTGTACTCTAC

940 SEQ ID N0:5488 -4.5 -22.2 66.6 -17.7 0 -4.9 AAAATCTTCTAAGCAAAACC

1001 SEQ ID N0:5489 -4.5 -16.2 51.1 -11.7 0 -4.1 AGAAACGTAAAATCTTCTAA

1009 SEQ ID N0:5490 -4.5 -14.7 48.5 -10.2 0 -5.3 GGTCCACAAACTCTGTCACA

1077 SEQ ID N0:5491 -4.5 -24.6 70.8 -19.4 -0.5 -3 GAGTCATGAGTCGAAATAGA

1596 SEQ ID N0:5492 -4.5 -19.4 59.5 -13.4 0 -10.9 CACCACAGCCAGGATCAAAT

1721 SEQ ID N0:5493 -4.5 -24.5 67.7 -20 0 -5.3 CTGTCTCTCTCTCCTTTGTT

2017 SEQ ID N0:5494 -4.5 -26.1 79.7 -21.6 0 0 TTCAGATTCGGATTTGGGAA

2039 SEQ ID N0:5495 -4.5 -21.4 63.2 -16.9 0 -5 AAAGGAAGCTGCTTCTTTTG

2067 SEQ ID N0:5496 -4.5 -20.8 62.5 -13.1 -2 -14.4 CTGGTCAGTCTGTTTCCATC

2095 SEQ ID N0:5497 -4.5 -25.8 78 -20.8 -0.1 -4.4 CCCTGGCACCATCCTGGATG

2363 SEQ ID N0:5498 -4.5 -30.6 80.2 -24.4 -1,2 -11.2 GAGCTGAAGGTCCACCCACC

2439 SEQ ID N0:5499 -4.5 -29.8 79,8 -24.4 -0.8 -5.8 CAATGATCTTGAGAACCATT

2889 SEQ ID N0:5500 -4.5 -19.5 58.5 -15 0 -7.1 CAATCCCTCCACATTTGACA ' 2993 SEQ ID N0:5501 -4.5 -25 69.2 -20.5 0 -2.4 GAGCCCCCACAGAATTGCCA

3096 SEQ ID N0:5502 -4.5 -30.5 78,7 -26 0 -3.2 GCATGCCGACCACAGCAAAA

3156 SEQ ID N0:5503 -4.5 -25.9 68.3 -20.3 -1 -8.1 ATACAGTCCCACATGGTCTC

3307 SEQ ID N0:5504 -4.5 -25.9 75.3 -21.4 0 -5.2 TTTCGCTGTTCAATGTATAT

4153 SEQ ID N0:5505 -4,5 -20.4 62.1 -15.9 0 -3.1 AAGCATTTCCAGAATGAATA

4220 SEQ ID N0:5506 -4.5 -18.6 56.6 -12.5 -1.6 -4.9 CTTAATGTCCGTAATGATTT

4372 SEQ ID N0:5507 -4.5 -19.4 58.5 -14.9 0 -2.6 GCTCTTAATGTCCGTAATGA

4375 SEQ ID N0:5508 -4.5 -22.3 65.1 -17.8 0 -2.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ACATTCATGATAGAGGGAAT

4465 5EQ ID N0:5509 -4.5 -19.3 59.2 -14.8 0 -6.4 GTTAACATCACTAATGTCAA

4589 SEQ ID N0:5510 -4.5 -18 56.4 -11.9 -1.5 -6.6 GCAGGAAGGGACATCATCAA

5413 SEQ ID N0:5511 -4.5 -23 66.6 -17.9 -0.3 -3.7 TCAAAGTTGAACATGTCATC

5530 SEQ ID N0:5512 -4.5 -18.5 57.8 -13.4 -0.3 -7 AAAAAGAAAATCCCAACAGA

5704 SEQ ID N0:5513 -4.5 -15.6 49 -11.1 0 -2 ACATGTTCACCACAACCAGG

5748 SEQ ID N0:5514 -4.5 -24.5 68.8 -19.3 -0.4 -6.1 ACACAAAACACGCTTTGTAA

6038 SEQ ID N0:5515 -4.5 -18.7 55.7 -12.7 -1.4 -6.6 TAAGTCAATCCTCCCTTTAA

6266 SEQ ID N0:5516 -4.5 -22.4 64.9 -17.9 0 -2.4 TTTTCCTTTGCTTTCTTTTT

6431 SEQ ID N0:5517 -4.5 -22.4 68.4 -17.9 0 -3.6 CAAAGATAATTCTTTGTTTC

6478 SEQ ID N0:5518 -4.5 -16.3 53.2 -8.4 -3.4 -8.4 TGACTATGAGTATTTGTTAA

6580 SEQ ID N0:5519 -4.5 -17.4 56 -12.4 -0.1 -3 CTTCACAGAGTTGCAGTGAC

6632 5EQ ID N0:5520 -4.5 -23.4 70.7 -17.4 -1.4 -7.4 AAAAATAGTGACATAGCATT

6888 SEQ ID N0:5521 -4.5 -15.9 51.2 -11.4 0 -4.1 CTAGAAAATGGAAGTAACTG

7038 SEQ ID N0:5522 -4.5 -15.5 50.3 -11 0 -3 ACAGGCACATAACTGTTCAG

7105 SEQ ID N0:5523 -4.5 -22 65.5 -16.2 -1.2 -6.7 ATGTGACATTCTTTAACCAA

7613 SEQ ID N0:5524 -4.5 -19.5 59 -15 0 -3.5 TGAATTAAAAGTGCAAAAGT

7711 SEQ ID N0:5525 -4.5 -14.4 47.8 -9.9 0 -5.4 TGTCATCAACCTGAAAATAA

7902 SEQ ID N0:5526 -4.5 -17.3 53.5 -12.8 0.4 -3 TACTACAAAAGTTGAATAAA

8171 SEQ ID N0:5527 -4.5 -12.8 44.9 -7.7 -0.3 -4.2 GTTCACAAAGCTGCTTTTAA

8661 SEQ ID N0:5528 -4.5 -20.6 62 -14.6 -0.3 -10.9 ATTAGTTACAATTTTTTTTC

72 SEQ ID N0:5529 -4.4 -16.1 53.7 -11.7 0 -2.6 CTGATAAAACAGAGCCTTAT

205 SEQ ID N0:5530 -4.4 -19.1 57.5 -14.7 0 -4.5 GAATGTCTCCATAAATAAAT

657 SEQ ID N0:5531 -4.4 -16.1 51.3 -11.1 -0.3 -2.8 AATAGTGCACATGATAAGCA

866 SEQ ID N0:5532 -4.4 -19.7 59.6 -14.3 -0.4 -9.9 GCCCAATGAGAGCAAACACG

1248 SEQ ID N0:5533 -4.4 -24 65 -19.6 0 -4.1 GCTCATTGTTACATTAACAA

1385 SEQ ID N0:5534 -4.4 -19.1 58.7 -12.6 -2.1 -8 CGAAATAGAGACAGGAAAGC

1585 SEQ ID N0:5535 -4.4 -18 54.7 -13.6 0 -2.8 CTGCTGAAATTCGGCCTCTT

1793 SEQ ID N0:5536 -4.4 -25.4 70.6 -19 -2 -10.2 CTGGATGACATACTGGCCTG

2350 SEQ ID N0:5537 -4.4 -24.9 70.4 -20.5 0 -7.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TAGATTCTTCAAGTTCTTCC

2619 SEQ ID N0:5538 -4.4 -21.3 66.2 -16.4 -0.2 -3.6 TAAATTCACAAGATGTTTTA

2717 SEQ ID N0:5539 -4.4 -15.5 51 -11.1 0 -4.3 CACTACTGAATTGCTCAGTC

2823 SEQ ID N0:5540 -4.4 -22 66.4 -14.8 -2.8 -8.2 CTGAGGCTGACAATAATTCC

2956 SEQ ID N0:5541 -4.4 -21.7 63.5 -17.3 0 -2.9 TTTTCACATAATCAATTCCC

3510 SEQ ID N0:5542 -4.4 -20.2 60.4 -15.8 0 -2.5 CAGTATTATTGGACATGCAG

3612 SEQ ID N0:5543 -4.4 -20.7 62.8 -16.3 0 -5.5 AGGATCATGAACACAATGAA

4102 SEQ ID N0:5544 -4.4 -17.8 54.8 -12.5 -0.8 -7.4 ATGTATATATCTTCAAAGGC

4141 SEQ ID N0:5545 -4.4 -18.4 58 -14 0 -4.2 CCAGCCTTTAAATGTGGCCA

4709 SEQ ID N0:5546 -4.4 -26.8 72.6 -20.6 -1.8 -8.8 CCATGTTGAGGCAGATGAGG

5073 SEQ ID N0:5547 -4.4 -24.7 71.1 -20.3 0 -4.3 CAAAGATGTTCCAGCCTATA

5229 SEQ ID N0:5548 -4.4 -22.4 64.9 -18 0 -3.2 CCTTTGCTCCTTTGATCAGA

5370 SEQ ID N0:5549 -4.4 -25.6 73.6 -21.2 0 -6.5 GCCGATGTTAAACAACGCAG

5429 SEQ ID N0:5550 -4.4 -22.5 62.2 -18.1 2.6 -6 CTTTTTAACATAGGCAAAGT

5498 SEQ ID N0:5551 -4.4 -18.4 57 -14 0 -4 GGTG~GTGCACTATTAAGAAT

5623 SEQ ID N0:5552 -4.4 -21.1 63.3 -15.1 -1.1 -10.8 ATGTACATGTTCACCACAAC

5752 SEQ ID N0:5553 -4.4 -21.5 63.7 -16.4 -0.4 -7.6 ATCAAACTTTT'CCCAAACCT

5855 SEQ ID N0:5554 -4.4 -21.9 62 -17.5 0 -2.5 AGCTGCAAAATCAGAGAGTT

5906 SEQ ID N0:5555 -4.4 -20.7 62.3 -15.6 -0.4 -6.5 TCTCTCCACTCTCACACAAA

6051 SEQ ID N0:5556 -4.4 -23.6 68.8 -19.2 0 0 AGACTTTGGAGGGGTTTGAT

6111 SEQ ID N0:5557 -4.4 -23.3 69.5 -18.9 0 -2.6 CCTTTGCTTTAAAAGATAAC

6209 SEQ ID N0:5558 -4.4 -17.4 54.1 -12.2 -0.6 -4.8 CTTGATATATTTTTTAACCT

6226 SEQ ID N0:5559 -4.4 -17.6 55.6 -13.2 0 -4.3 TGGTTTGTCTTTCTCAAACT

6410 SEQ ID N0:5560 -4.4 -21.4 65.6 -13.8 -3.2 -8.3 CCATTGGGTTTCTCCTCAGC

6703 SEQ ID N0:5561 -4.4 -28.1 80.5 -22.8 -0.8 -5 ACAACTATCCCTATAGTCTA

6728 SEQ ID N0:5562 -4.4 -21.9 65.3 -15.5 -2 -5.4 TACTGTTTCTTCAACGTAAA

6918 SEQ ID N0:5563 -4.4 -18.5 57.2 -13 -l -5.3 AATATTAGAGGAGACTTTAC

7123 SEQ ID N0:5564 -4.4 -17 55 -11.7 -0.7 -6.7 ATATTTGATTTAATAGAAGT

7450 SEQ ID N0:5565 -4.4 -14.5 49.3 -10.1 0 -2.6 AAACAAGTTTTAGACATATT

7821 SEQ ID N0:5566 -4.4 -15.3 50.5 -10.2 -0.4 -5.l kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTGCAGCAGGAATTAAAAAA

8215 SEQ ID N0:5567 -4.4 -17.6 53.9 -13.2 0 -6.6 TTTTATTAATTATATATATT

8542 SEQ ID N0:5568 -4.4 -12.1 44.3 -7.7 0 -5.2 TTATTATCAAACCTGAAAGT

8717 SEQ ID N0:5569 -4.4 -16.8 53.1 -11.7 -0.4 -2.9 ATCAATTATATTGAAAGAAG

8805 SEQ ID N0:5570 -4.4 -13.1 45.8 -6.7 -2 -6.6 AATGTAACATTTATTAGCCA

8845 SEQ ID N0:5571 -4.4 -18.6 57.2 -13.7 -0.2 -5.5 TATATAATGCATTTAAGTAA

8870 SEQ ID N0:5572 -4.4 -14.3 48.4 -9.3 0 -8.5 CTCTGTGGATAAGAAAACAA

45 5EQ ID N0:5573 -4.3 -16.9 53 -12.6 0 -2.9 CTTCCAAGTCACTATTTGGC

621 SEQ ID N0:5574 -4.3 -23.7 69.8 -17.9 -1.4 -5.1 GCACTGAATCGGAAAATTGC

763 SEQ ID N0:5575 -4.3 -20.3 58.9 -16 0 -4.3 CTCTACATTCTTTGTCCAGT

926 SEQ ID N0:5576 -4.3 -23.9 72.3 -19 -0.3 -3.5 GTGTACTCTACATTCTTTGT

931 SEQ ID N0:5577 -4.3 -21.9 68.4 -16.7 -0.8 -5 GTTCTCAACGCTGAGACATT

1102 SEQ ID N0:5578 -4.3 -22.9 67 -15.8 -2.8 -8.2 TTCCAAGGTGGCCTGATTCT

1760 SEQ ID N0:5579 -4.3 -26.8 75.7 -21.6 -0.7 -7.2 GCTGAAATTCGGCCTCTTTT

1791 SEQ ID N0:5580 -4.3 -24.7 69.5 -19 -1.3 -9.2 CTTCAGATTCGGATTTGGGA

2040 SEQ ID N0:5581 -4.3 -23 67.3 -18.7 0 -5 CCCGACCTCTGAAACTGAAA

2202 SEQ TD N0:5582 -4.3 -23.3 63.1 -19 0 -2.8 AAGAGCTTAACCTTCTCTTT

2517 SEQ ID N0:5583 -4.3 -21.7 65.2 -15.8 -1.5 -5.5 GCGGACATTTCTGTCTAGAT

2634 SEQ ID N0:5584 -4.3 -23.6 69.6 -16.6 -2.7 -8 AGATGTTTTACTTTTAACCA

2707 SEQ ID N0:5585 -4.3 -19.1 59.1 -14.8 0 -2.8 ATGATCTTGAGAACCATTTC

2887 SEQ ID N0:5586 -4.3 -20 61 -15.7 0 -5.1 GAAAACTCTAAGCAGTCTGA

3029 SEQ ID N0:5587 -4.3 -19.5 59.5 -15.2 0 -4.1 AAAGAGCTGCATGCCGACCA

3164 SEQ ID N0:5588 -4.3 -26.5 70.8 -21.5 0 -9.1 ACAGTCCCACATGGTCTCTA

3305 SEQ ID N0:5589 -4.3 -26.8 77.4 -22.5 0 -5.2 TACAGTCCCACATGGTCTCT

3306 SEQ ID N0:5590 -4.3 -26.8 77.4 -22.5 0 -5.2 CTACACCACTGGTGGTTCCA

3675 SEQ ID N0:5591 -4.3 -27.5 77 -20.6 -2.6 -12 GAAACTTTTTAATACACCCT

3984 SEQ ID N0:5592 -4.3 -19.4 57.8 -15.1 0 -2.5 TCAAAGGCCAATGCACCACT

4129 SEQ ID N0:5593 -4.3 -25.2 68.7 -19.4 -1.4 -6.9 AGCATTGGCTACCAGGCTAA

4325 SEQ ID N0:5594 -4.3 -26 73.3 -20.1 -1.5 -7.1 TTAGAGGTCTTAAAGCTCTT

4389 SEQ ID N0:5595 -4.3 -20.6 64 -15.1 -1.1 -5.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CATGCCTTCAAACCGGGATA

4415 SEQ ID N0:5596 -4.3 -24.9 67.5 -20.6 0 -7.1 CATCTCGTGAATCAACAGCT

4746 SEQ TD N0:5597 -4.3 -22.3 64.9 -18 0 -4.6 TAAATACATGTACAGATTTT

4790 SEQ ID N0:5598 -4.3 -15.6 51.2 -10.8 0 -8.2 TCCAAGTTTCTTCATTGCAT

4952 SEQ ID N0:5599 -4.3 -22.9 68.2 -18.6 0 -5.1 ATCAAAGACCATTCCTTGGA

5015 SEQ TD N0:5600 -4.3 -22.5 64.8 -16.6 -1.5 -4.8 GTGAACAGAACAATGAACAC

5161 SEQ ID N0:5601 -4.3 -17.1 53.2 -12.8 0 -3.2 ATCATCTCAGCCAGAAACAT

5281 SEQ TD N0:5602 -4.3 -22.1 64.8 -17.8 0 -3.6 AGGAAGGGACATCATCAAAG

5411 SEQ ID N0:5603 -4.3 -19.8 59.6 -14.9 -0.3 -2.9 AACATGTCATCAATTCCAGC

5521 SEQ ID N0:5604 -4.3 -21.9 64.7 -17.6 0 -6.9 TTGAACATGTCATCAATTCC

5524 SEQ ID N0:5605 -4.3 -20.1 60.7 -14.9 -0.5 -9.1 GAAAATCCCAACAGATGGGT

5699 SEQ ID N0:5606 -4.3 -22 62.3 -14.5 -3.2 -7.9 GGGTTTTGCTATGAGAAGAG

5939 SEQ ID N0:5607 -4.3 -21.4 64.9 -17.1 0 -3.6 GGTTTGATGCCATAAACCTG

6099 SEQ ID N0:5608 -4.3 -23.1 65.5 -16.5 -2.3 -8.6 AATTACGCTGAATGATAGCG

6183 SEQ ID N0:5609 -4.3 -19.6 57.6 -l3 -2.3 -7.6 ACTGTTTCTTCAACGTAAAC

6917 SEQ ID N0:5610 -4.3 -19 58.2 -13.6 -1 -6.2 TGTGATGTCATCAACCTGAA

7907 SEQ ID N0:5611 -4.3 -21.5 63.5 -16.1 -1 -6.9 AAATTATCCATTGAAAAATC

7992 SEQ ID N0:5612 -4.3 -13.8 46.6 -8.8 -0.5 -4 GGAATGGTAGAAAATATATT

8058 SEQ ID N0:5613 -4.3 -15.2 49.9 -10.9 0 -5.9 TTGGAATGGTAGAAAATATA

8060 SEQ ID N0:5614 -4.3 -15.2 49.9 -10.9 0 -2.7 AAAAATAAGACTAGTCTGCA

8199 SEQ ID N0:5615 -4.3 -16.6 52.8 -10.6 0 -11.5 TAAAAAGAACAAAGTCCATG

8255 SEQ ID N0:5616 -4.3 -14.6 47.9 -10.3 0 -3.9 TP~AAAAATGAAATTTATTCT ' 8460 SEQ ID N0:5617 -4.3 -10.7 41 -5.1 -1.2 -5.2 ATAGCATCAATTATATTGAA

8810 SEQ TD N0:5618 -4.3 -16.1 52.2 -9.8 -2 -8.1 CTCAATAACTATATCATTAA

8913 SEQ ID N0:5619 -4.3 -15 49.8 -10.7 0 -2.6 AAAAATATGCATTACAACTG

9013 SEQ ID N0:5620 -4.3 -14.5 47.9 -10.2 0 -6.8 TATGGGAGAATTATGAAATA

9057 SEQ ID N0:5621 -4.3 -15.3 50.1 -11 0 -3.2 CAGAATCCTCTCTGCTTATG

14 SEQ ID N0:5622 -4.2 -23 68 -18.1 -0.4 -3.7 TTTCCAGAATCCTCTCTGCT

l8 SEQ ID N0:5623 -4.2 -25.8 74.6 -20.6 -0.9 -4.5 GCATCCAAGATGGTTAGGGT

162 SEQ ID N0:5624 -4.2 -25.3 73.1 -19.6 -1.4 -6.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGCCCAGCATCCAAGATGGT

168 SEQ ID N0:5625 -4.2 -28.4 77.5 -22.6 -1.5 -7 TCTGATAAAACAGAGCCTTA

206 SEQ ID N0:5626 -4.2 -19.5 58.8 -14.1 -1.1 -5.9 TAAAAAGGCGGAAGCTTTCA

504 SEQ ID N0:5627 -4.2 -19.8 58.1 -13.2 -2.4 -7.6 ATAGTGCACATGATAAGCAT

865 SEQ ID N0:5628 -4.2 -20.4 61.6 -14.9 -1.1 -9.4 ATTCCAGTGAATGTGTACTC

943 SEQ ID N0:5629 -4.2 -21.9 66.5 -16.8 -0.7 -6.2 ATATGCCATCACAATGACAC

1058 SEQ ID N0:5630 -4.2 -21.2 62 -16.3 -0.4 -4.2 TGCAGCCCAATGAGAGCAAA

1252 SEQ ID N0:5631 -4.2 -24.6 67.6 -19.6 -0.6 -5.4 TGCTCATTGTTACATTAACA

1386 SEQ ID N0:5632 -4.2 -19.8 60.7 -14.1 -1.4 -6.4 TTAAATGTGCTCATTGTTAC

1393 SEQ ID N0:5633 -4.2 -18.7 58.6 -12.9 -1.6 -4 ATAAAATGAGCCCAAGAAAA

1694 SEQ ID N0:5634 -4.2 -16.5 50.8 -12.3 0 -3.2 TTCTTTAGCACCTTTGGAAC

1946 SEQ ID N0:5635 -4.2 -22.4 66.4 -18.2 0 -4.1 TCTTCAGATTCGGATTTGGG

2041 SEQ ID N0:5636 -4.2 -22.8 67.5 -18.6 0 -5 AAAAGGAAGCTGCTTCTTTT

2068 SEQ ID N0:5637 -4.2 -20.1 60.5 -12.4 -2.3 -l5 GGCACCATCCTGGATGACAT

2359 SEQ ID N0:5638 -4.2 -27.2 74.7 -21.3 -1.2 -11.3 CCACACCATTGCAATCCACA

2412 SEQ ID N0:5639 -4.2 -26.4 70.9 -21.6 0 -8.6 AAGATGTTTTACTTTTAACC

2708 SEQ ID N0:5640 -4.2 -17.7 55.9 -13.5 0 -2.8 TTTCTGCTGTGAAAATCCCA

2871 SEQ ID N0:5641 -4.2 -23 65.7 -18.3 -0.2 -4 TATTTAGTGTGGGCCAGGAT

3060 SEQ ID N0:5642 -4.2 -24.7 72.3 -20 0 -7.6 TTGCATTTAATTTCTCTTTG

3858 SEQ ID N0:5643 -4.2 -18.8 59.3 -14.6 0 -5.1 GTAAAGACTTTGTCAGCATA

4195 SEQ ID N0:5644 -4.2 -19.9 61.4 -l5 -0.4 -4.6 TGGATCCAAGTTTCTTCATT

4956 SEQ ID N0:5645 -4.2 -22.2 66.7 -17.1 0 -9.6 TGCTGATATCAAAGACTTGT

5046 SEQ ID N0:5646 -4.2 -19.7 60.2 -14.9 -0.3 -7.7 TACTTTTCTATCATCTCAGC

5290 SEQ ID N0:5647 -4.2 -21.4 66.9 -17.2 0 -2.8 ATTCCAGCTTCCTTTTTAAC

5509 SEQ ID N0:5648 -4.2 -22.9 67.6 -18.7 0 -4.3 AGGGTCACAGTCGGGTGGTG

5636 SEQ TD N0:5649 -4.2 -28 81 -23.8 0 -5.6 CCAAACCTCATAGAACATCT

5843 SEQ ID N0:5650 -4.2 -21.3 61.5 -17.1 0 -2.6 CTCTATAAACTGGGTCGCAT

5879 SEQ ID N0:5651 -4.2 -22.6 65.4 -18.4 0 -3.6 CTCCCTTTAATTGCCTCTTT

6256 SEQ ID N0:5652 -4.2 -25.7 72.6 -21.5 0 -3 GGTTTGTCTTTCTCAAACTT

6409 SEQ ID N0:5653 -4.2 -21.5 66.1 -14.1 -3.2 -8.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAGTCCAGTTGACACATATA

6530 SEQ ID N0:5654 -4.2 -20.7 62.7 -14.7 -1.8 -4.9 ACATAACAGACTAAAATACT

7075 SEQ ID N0:5655 -4.2 -14.9 49 -10.7 0 -2 TAGATTACTTTTTGGAAAGC

7233 SEQ ID N0:5656 -4.2 -17.8 56.3 -12 -1.6 -5.2 AGAAGATGGCAAAGCAATAC

7320 SEQ ID N0:5657 -4.2 -18.5 56.2 -13.4 -0.7 -4.3 AAGCTTGCAAAAGACACATA

7481 SEQ ID N0:5658 -4.2 -18.6 56.3 -12.3 -2.1 -6.9 TGAATGATACAGGATACATC

7509 SEQ ID N0:5659 -4.2 -17.9 56.1 -13.7 0 -3.2 AAGTGCAAAAGTAAAGGTGT

7703 SEQ ID N0:5660 -4.2 -18 55.7 -13.8 0 -5.4 TAAAGTTGATTATATTTTTA

7855 SEQ ID N0:5661 -4.2 -13.8 47.9 -9.6 0 -2.6 TGCTGACAAATAAAGTTGAT

7865 SEQ ID N0:5662 -4.2 -17 53.3 -12.3 -0.1 -3.7 ATGCTGACAAATAAAGTTGA

7866 SEQ ID N0:5663 -4.2 -17 53.3 -12.3 -0.1 -3.7 TTCAAAAGTTTGGAATTGTG

7965 SEQ ID N0:5664 -4.2 -16.9 53.8 -12.2 -O.l -6.9 TGGAATGGTAGAAAATATAT

8059 SEQ ID N0:5665 -4.2 -15.1 49.6 -10.9 0 -3.9 AAAATAAGACTAGTCTGCAT

8198 SEQ ID N0:5666 -4.2 -17.3 54.5 -11.4 0 -11.5 ACTGGAGTTTTCCACTGAGA

8997 SEQ ID N0:5667 -4.2 -23.8 70.4 -17.5 -2.1 -6.3 CTGTCAAACCTTGATGTGGC

254 SEQ ID N0:5668 -4.l -23.8 68.5 -18.5 -1.1 -5 CCAACAGTGCCTGTGCCATC

471 SEQ ID N0:5669 -4.1 -28.9 78.6 -23.2 -1.5 -8.7 TGCCAAGATTTTTATAAGTG

977 SEQ ID N0:5670 -4.1 -18.7 57.7 -14.6 0 -4.l CTCATTGTTACATTAACAAA

1384 SEQ ID N0:5671 -4.1 -16.6 53 -9.7 -2.8 -8.5 AATTCACCAAATAAAATGAG

1704 SEQ ID N0:5672 -4.1 -14.2 47.1 -10.1 0 -3 AGCTCTCCTAACCCACCTAC

1894 SEQ ID N0:5673 -4.1 -28.2 77 -24.1 0 -4.3 GTTTCCTTCAAGGTGCTCTC

1997 SEQ ID N0:5674 -4.1, -26 77.8 -21.1 -0.6 -5.3 TTCAGATCCAACATCCTTTG

2222 SEQ ID N0:5675 -4.1 -22.6 66 -18 -0.1 -4.5 TACCAGCATGGCGGACATTT

2644 SEQ ID N0:5676 -4.1 -25.8 71.2 -19.7 -2 -7.4 AAATCTATACCAGCATGGCG

2651 SEQ ID N0:5677 -4.1 -22.5 63.8 -16.4 -2 -7.4 GTTTCCTACAGTCAACACAC

2840 SEQ ID N0:5678 -4.1 -22.9 68.1 -18.3 -0.1 -3 AGGTTTCCTACAGTCAACAC

2842 SEQ ID N0:5679 -4.l -23.2 69.2 -18.6 -0.2 -3.6 AAAGACCAGGTTTCCTACAG

2849 SEQ ID N0:5680 -4.1 -22.6 65.7 -18 -0.1 -5.9 CCATTTCTGCTGTGAAAATC

2874 SEQ ID N0:5681 -4.1 -21 62.1 -16 -0.7 -5 CTTCTTGGAAATAGTAATAA

2919 SEQ ID N0:5682 -4.1 -15.7 51.3 -11.6 0 -3.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CTAAGCAGTCTGAATGATCG

3022 SEQ ID N0:5683 -4.1 -20.6 61.5 -15.8 -0.4 -5.6 GGAGCGTACAGTCATCATTG

3216 SEQ ID N0:5684 -4.1 -23.7 69.9 -19.6 0 -5 TCTAAAAAAGGCTTTTTGGA

3548 SEQ ID N0:5685 -4.1 -17.6 54.7 -11.4 -2.1 -7.7 AACATCAACTGTGCTTCCTT

3890 SEQ ID N0:5686 -4.1 -23.1 67.2 -18.3 -0.4 -4.1 GGGTAGAACAACATCAACTG

3899 SEQ ID N0:5687 -4.1 -19.8 59.4 -15.7 0 -2.9 TGGAAACTTTTTAATACACC

3986 SEQ ID N0:5688 -4.1 -17.7 54.7 -13.6 0 -2.8 GAGTAGCCAAGAGCATTGGC

4336 SEQ ID N0:5689 -4.1 -25.5 73.4 -17.8 -3.6 -12 TGATGGCACCGAGTTCTGAG

4353 SEQ ID N0:5690 -4.1 -25.1 71.2 -21 0 -3.9 AAAGATGACAAAGTATAAAT

4805 SEQ ID N0:5691 -4.1 -12.5 44.2 -8.4 0 -2.8 CCGTTTCCACCATCATGGTG

5094 SEQ ID N0:5692 -4.1 -27.7 75.3 -20.3 -3.3 -10.5 CAAGCAGATCATGCTGTTGC

5558 SEQ ID N0:5693 -4.1 -24 70.2 -17.1 -2.8 -10.6 TAGCAATCCATCCCAGCCAG

5597 SEQ ID N0:5694 -4.l -28.2 76.2 -24.1 0 -4.1 AACTTTTCCCAAACCTCATA

5851 5EQ TD N0:5695 -4.1 -22.3 63.4 -18.2 0 -1.7 GACAGTGGATCCGGTCACCA

6000 SEQ ID N0:5696 -4.1 -28.1 77.3 -22.8 -0.7 -10.1 CTGAAATTACGCTGAATGAT

6187 SEQ ID N0:5697 -4.1 -18.1 54.8 -14 0 -3.3 .

GGACTGTACTAAAGGTGTTT

6773 5EQ ID N0:5698 -4.1 -21 63.9 -16.9 0 -4.1 ATTTACAAAAATAGTGACAT

6894 SEQ ID N0:5699 -4.1 -14.4 48.2 -9.7 -0.3 -4.8 TAAAATAAATTGTGATGTCA

7917 SEQ ID N0:5700 -4.1 -14.1 47.6 -10 0 -4.9 CAATAATGACGTAGCTCACC

8433 SEQ ID N0:5701 -4.l -21.5 62.2 -17.4 0 -5.3 ATAACTATATCATTAATACC

8909 SEQ ID N0:5702 -4.1 -15.6 51 -11.5 0 -4.2 TTATGAAATACATATTTAGA

9047 SEQ ID N0:5703 -4.1 -13.8 47.4 -8.6 -1 -4.6 ATAACAAAGCCCAGCATCCA

175 SEQ ID N0:5704 -4 -24.6 67.2 -19.7 -0.7 -4.1 TTATGAATTACAGCATAACA

189 SEQ ID N0:5705 -4 -16.8 53.3 -11.9 -0.8 -4.9 CTGGAGGAATGTCTCCATAA

663 SEQ ID N0:5706 -4 -22.4 65.5 -15.1 -3.3 -8.5 ACTGAATCGGAAAATTGCCT

761 SEQ ID N0:5707 -4 -20.7 59.3 -16.7 0 -4.4 CCGACTGGATCAGGGCCCCC

1182 SEQ ID N0:5708 -4 -33.6 84.5 -28.2 -0.7 -10.5 AGAACACAGTCAGGATCATC

1221 SEQ ID N0:5709 -4 -21.3 65 -17.3 0 -5.3 TGTAATCCTTCCAGTTAAAT

1407 SEQ ID N0:5710 -4 -20.3 60.9 -16.3 0 -2.8 ACTGTCATCTCCAATGTAAT

1421 SEQ ID N0:5711 -4 -21.3 63.9 -17.3 0.3 -3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTTGCCCATCCAAAACATAA

1446 SEQ ID N0:5712 -4 -21.6 60.7 -17.1 -O.l -3.4 ATAGTTGGGGTTTCGACCAG

1535 SEQ ID N0:5713 -4 -24.7 71.3 -18 -2.7 -6.4 AACTGGTAAAGATTTTCCCA

1624 SEQ ID N0:5714 -4 -21.1 61.8 -17.1 0 -4.6 TGCCGCAACTGCCTGAGCTT

1844 SEQ ID N0:5715 -4 -29.6 78.3 -24.4 -1.1 -7.1 AACTTTGATGCTTCTGAAGA

1924 SEQ ID N0:5716 -4 -19.8 60.4 -15.3 0 -8.3 GCGGCACAAACAGTGAGTCT

2295 SEQ ID N0:5717 -4 -25 70.7 -19.4 -1.5 -7 TGGCACCATCCTGGATGACA

2360 SEQ ID N0:5718 -4 -27.2 74.6 -21.3 -1.2 -11.8 TTAAGACAATGCAAATAGTG

2769 SEQ ID N0:5719 -4 -15.9 51.2 -11.4 -0.1 -5.6 TAGTAATAAGGATCCATGGC

2908 SEQ TD N0:5720 -4 -20.8 62.3 -15.9 0 -9.5 TCTTAATTAGCATATTTAGT

3072 SEQ ID N0:5721 -4 -17.5 56.7 -13.5 0 -4.1 AAGAGGTTCAGAACCACAAG

3388 SEQ ID N0:5722 -4 -20.5 61 -13.8 -2.7 -12 ATCTGCAGATTATTCATTTC

3469 SEQ ID N0:5723 -4 -20 62.7 -14.3 -0.2 -11.6 TTTGCATTCTTCCTACTGCA

3489 SEQ ID N0:5724 -4 -24.8 72.4 -19.3 -1.4 -5.4 CTGTCTATCTTATTGCCTTC

3592 SEQ ID N0:5725 -4 -23.5 71.2 -19.5 0 -3 TACCTACACCACTGGTGGTT

3678 SEQ ID N0:5726 -4 -26.3 74.2 -19.7 -2.6 -12 TCATCGATTACGTATTTTTC

3712 SEQ ID N0:5727 -4 -19.4 59.6 -14.5 -0.7 -6.3 GTTTATGAATGACATATAAT

3737 5EQ ID N0:5728 -4 -15.2 50.3 -10.1 -1 -5.1 GATGAGCTGGTTGCATTTAA

3868 SEQ ID N0:5729 -4 -22.1 65.8 -17.2 -0.7 -5.1 CAGATGAGCTGGTTGCATTT

3870 SEQ ID N0:5730 -4 -23.8 70.2 -18.2 -1.5 -6.1 TTCCTTCAGATGAGCTGGTT

3876 SEQ ID N0:5731 -4 -25 74 -19.8 -1.1 -5 TCAACTGTGCTTCCTTCAGA

3886 SEQ TD N0:5732 -4 -24.6 72.2 -19.7 -0.7 -4 CATCAACTGTGCTTCCTTCA

3888 SEQ ID N0:5733 -4 -24.7 71.7 -20.7 0 -3.6 CAACATCAACTGTGCTTCCT

3891 SEQ ID N0:5734 -4 -23.7 68 -19 -0.4 -4.1 CGGGGTAGAACAACATCAAC

3901 SEQ ID N0:5735 -4 -20.9 60.6 -16.9 0 -2.9 TGAACACAATGAAAGTCTCA

4095 SEQ TD N0:5736 -4 -17.8 55.1 -13.3 -0.1 -3.3 TATAGGTAAAGACTTTGTCA

4200 SEQ ID N0:5737 -4 -18.3 58 -13 -1.2 -5.2 TCCATAAGCAACCCATTTGA

4241 SEQ ID N0:5738 -4 -23.6 65.9 -18.9 -0.4 -4.1 GCATTGGCTACCAGGCTAAC

4324 SEQ ID N0:5739 -4 -26.2 73.6 -20.9 -1.2 -7.2 AGCTCTTAATGTCCGTAATG

4376 SEQ ID N0:5740 -4 -21.7 64 -17.7 0 -4.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGTCATGTTAACACAGTGGT

4556 SEQ ID N0:5741 -4 -22 66.8 -17.3 -0.3 -8.2 TCATAAAGATGTCTTGACCT

4908 SEQ ID N0:5742 -4 -20.3 61.4 -15.5 -0.6 -6.3 TGAACAGAACAATGAACACT

5160 SEQ ID N0:5743 -4 -16.8 52.3 -12.8 0 -3.l CATCTCAGCCAGAAACATAC

5279 SEQ ID N0:5744 -4 -21.6 63.4 -17.6 0 -3.6 TGCTCCTTTGATCAGACGTA

5366 SEQ ID N0:5745 -4 -24.4 70.6 -20.4 0 -6.5 ACATAGGCAAAGTTGGACAT

5491 SEQ ID N0:5746 -4 -20.9 62.1 -16.9 0 -4.2 CAAACTTTTCCCAAACCTCA

5853 SEQ ID N0:5747 -4 -22.6 63.1 -18.6 0 -2.4 ATATCAAGACAGTGGATCCG

6007 SEQ ID N0:5748 -4 -21.8 63.7 -17.1 0 -8.9 TCAGTTTGGCATGGACCTCC

6555 SEQ ID N0:5749 -4 -27.5 78 -23 -0.1 -5.4 TGTCAGCTGGTAATAAAAAC

6681 SEQ ID N0:5750 -4 -17.5 54.7 -12.9 0 -8.6 AAAATAGAATGGATGCAAGG

6791 SEQ ID N0:5751 -4 -16.3 51.4 -7.2.3 0 -5.6 TCTGGCAAAACACCTTTGTC

6966 SEQ ID N0:5752 -4 -22.7 65.7 -17.1 -1.5 -6.6 TTTCTGGTTTTGAGCAAAAA

6995 SEQ ID N0:5753 -4 -18.5 57 -13.7 -0.6 -8.1 TCAAAATTAAAGCCATCTAG

7054 SEQ ID N0:5754 -4 -16.9 52.9 -12.9 0 -3.4 AACTATTTACATAACATAGG

7378 SEQ ID N0:5755 -4 -15.7 51.3 -11.2 -0.1 -4.2 TTAAAAGTGCAAAAGTAAAG

7707 SEQ ID N0:5756 -4 -12.8 44.8 -8.8 0 -5.4 CATATTTTTAGCAAAATATG

7807 SEQ ID N0:5757 -4 -14.6 48.7 -6.8 -3.8 -10.4 GAAAATATATTATAACAAAG

8049 SEQ ID N0:5758 -4 -10.2 40 -6.2 0 -6.2 TGGAACATGTGAACTTGATC

8697 SEQ TD N0:5759 -4 -19.3 58.9 -14.6 -0.4 -7 TACTATTATTATCAAACCTG

8722 SEQ ID N0:5760 -4 -16.9 53.7 -12.9 0 -1.5 TAAAAATATGCATTACAACT

9014 SEQ ID N0:5761 -4 -14.2 47.4 -10.2 0 -6.8 AATTGTCTTTAAAAATATGC

9023 SEQ ID N0:5762 -4 -14.5 48.6 -10.5 0 -4.3 CTCTCTGCTTATGCCTCCGC

7 SEQ TD N0:5763 -3.9 -29.7 81.9 -24.9 -0.8 -4.6 TTTTTTTGACCACAGAGGTT

97 SEQ ID N0:5764 -3.9 -22.1 66.2 -16.6 -1.5 -4.7 ACCAACAGTGCCTGTGCCAT

472 SEQ ID N0:5765 -3.9 -28.7 77,5 -23.2 -1.5 -8.7 TTGTTCTCATCATCATTATC

595 SEQ ID N0:5766 -3.9 -20.2 64.1 -16.3 0 -1.9 TGATAGCAATTTTCCTAACA

819 SEQ ID N0:5767 -3.9 -19.6 59.2 -15.7 0 -3.3 ACTCTACATTCTTTGTCCAG

927 SEQ ID N0:5768 -3.9 -22.9 69.3 -19 0 -2,9 ACTCAAAGGTATAGATTCCA

957 SEQ ID N0:5769 -3.9 -20.4 61.7 -15.8 -0.4 -3.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGTCACATATGCCATCACAA

1064 SEQ TD N0:5770 -3.9 -22.7 65.8 -18.8 0 -5.6 AGAACTCTGAATGTTCTCAA

1114 SEQ ID N0:5771 -3.9 -19.2 59.4 -13.3 -2 -7.7 AAAGCTTCTTTACCGACTGG

1194 SEQ ID N0:5772 -3.9 -22.5 64.6 -17.9 -0.4 -7.6 TTCCTCAGATTGCCCATGAA

1276 SEQ TD N0:5773 -3.9 -25.6 71.3 -21.7 0 -4.5 GCCCAAGAAAATGACCAGGA

1685 SEQ ID N0:5774 -3.9 -23.6 64.4 -19.7 0 -4 ACCACAGCCAGGATCAAATT

1720 SEQ ID N0:5775 -3.9 -23.9 67 -20 0 -5.3 CTGAAATTCGGCCTCTTTTT

1790 SEQ ID N0:5776 -3.9 -23 65.9 -19.1 0 -6.7 CCTTTGGAACTCAACTTTGA

1936 SEQ TD N0:5777 -3.9 -21.6 63.2 -17.1 -0.3 -4.7 CTTTGTTGTTTCCTTCAAGG

2004 SEQ ID N0:5778 -3.9 -22.8 68.6 -18.3 -0.3 -4.6 TCAAGAGAGACTGATGAGGG

2133 SEQ ID N0:5779 -3.9 -20.9 63.2 -17 0 -2.8 ATCATCAGCAAAGTCATTTT

2240 SEQ ID N0:5780 -3.9 -19.9 61.3 -16 0 -4.1 TCCTGGATGACATACTGGCC

2352 SEQ ID N0:5781 -3.9 -26.4 73.8 -22.5 0 -6.2 CTTCCCATTTGCTGGAAGCC

2381 SEQ ID N0:5782 -3.9 -27.9 76.2 -21.8 -2.2 -7.9 TTAAATTCACAAGATGTTTT

2718 SEQ ID N0:5783 -3.9 -15.9 51.9 -12 0 -4.3 AACAATTAAATTCACAAGAT

2723 SEQ ID N0:5784 -3.9 -13.9 46.9 -10 0 -3.1 GACCAGGTTTCCTACAGTCA

2846 SEQ ID N0:5785 -3.9 -26.3 76.3 -21.5 -0.8 -5.9 TAAAGACCAGGTTTCCTACA

2850 SEQ ID N0:5786 -3.9 -22.3 64.9 -17.9 -0.1 -5.3 AATGATCTTGAGAACCATTT

2888 SEQ ID N0:5787 -3.9 -18.9 57.7 -15 0 -7.1 AATCCCTCCACATTTGACAG

2992 SEQ ID N0:5788 -3.9 -24.3 68.3 -20.4 0 -2.4 TTGAAAACTCTAAGCAGTCT

3031 SEQ ID N0:5789 -3.9 -19 58.5 -15.1 0 -4.1 CAAAAATGAAGACGATGATG

3141 SEQ ID N0:5790 -3.9 -14.6 47.5 -10.7 0 -3.5 AAGAGCTGCATGCCGACCAC

3163 SEQ ID N0:5791 -3.9 -27.4 73.5 -22.5 -0.8 -9.1 ACACAGCACGCGGAACACAA

3275 SEQ ID N0:5792 -3.9 -24.1 65.2 -19.7 0 -7.9 GTAGCCAAGAGCATTGGCTA

4334 SEQ ID N0:5793 -3.9 -25.5 73.2 -15.6 -6 -16 GAGTTCTGAGTAGCCAAGAG

4343 SEQ ID N0:5794 -3.9 -23.1 69.6 -19.2 0 -3.2 TTTGATGGCACCGAGTTCTG

4355 SEQ ID N0:5795 -3.9 -24.7 70.4 -20.8 0 -4 AGAGGGAATTGCTCCAACAA

4454 SEQ ID N0:5796 -3.9 -22.3 64 -16.6 -1.8 -6.1 GGAACAAGGTAGGGGACACA

5313 SEQ ID N0:5797 -3.9 -23.4 67.2 -18.9 -0.3 -3.2 CAGTAGCAACACTGAAGTTC

57g7 SEQ ID N0:5798 -3.9 -21 63.7 -16.2 -0.8 -5.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GATCCATGGCAATAAGCTGG

5967 SEQ ID N0:5799 -3.9 -23.6 67 -18.3 -1.3 -8 TTTTGTAAAAATATGGCAGA

6822 SEQ ID N0:5800 -3.9 -16.4 52.3 -11.2 -1.2 -6.3 ACTATTCTAAAGCAATACTT

7281 SEQ ID N0:5801 -3.9 -17.2 54.6 -12.7 -0.3 -4.1 AACATAGGGTATTTAATTGA

7366 SEQ ID N0:5802 -3.9 -17.1 54.5 -13.2 0 -2.9 GATACCATATGATACTCATG

7732 SEQ ID N0:5803 -3.9 -19.4 59.2 -14.6 -0.8 -5.5 AAAAAAAACATCTATGAATA

8153 SEQ TD N0:5804 -3.9 -10.6 40.6 -6.7 0 -3.8 TCATGTTAGGTAATTGCACA

8297 SEQ ID N0:5805 -3.9 -21 63.8 -17.1 0 -5.3 AAAAAACAAAAATTTATATC

8315 SEQ ID N0:5806 -3.9 -8.6 37.2 -4.7 0 -5.2 CTTCAATACACTACAGTAAA

8377 SEQ ID N0:5807 -3.9 -17.2 54.4 -13.3 0 -3.6 ATATTTTAAACTGCCAACAG

8527 SEQ ID N0:5808 -3.9 -18.1 55.4 -12.8 -1.3 -7.3 CTGGAGTTTTCCACTGAGAA

8996 SEQ ID N0:5809 -3.9 -22.9 67.5 -16.9 -2.1 -6.3 GCTCCATAATCTCTGATAAA

217 SEQ ID N0:5810 -3.8 -20.3 60.8 -16.5 0 -3.3 CCTGTCAAACCTTGATGTGG

255 SEQ ID N0:5811 -3.8 -24 68 -19 -1.1 -4.5 GTCCTGGGGGTACCAACAGT

483 SEQ ID N0:5812 -3.8 -28.5 79.7 -21.9 -2.8 -10.6 CAATTTTCCTAACAGGGTTT

813 SEQ ID N0:5813 -3.8 -21.3 63.3 -16.6 -0.7 -4.1 AATGAGAGCAAACACGCTCA

1244 SEQ ID N0:5814 -3.8 -21.3 61.3 -15 -2.5 -7.7 AGGATCAAATTCACCAAATA

1711 SEQ ID N0:5815 -3.8 -17.9 54.8 -13.5 -0.3 -5.3 CTGCTCCTCATAGGCCATGG

1742 SEQ ID N0:5816 -3.8 -28.6 79.4 -23.6 -0.3 -10.3 CTGATTCTGCTCCTCATAGG

1748 SEQ ID N0:5817 -3.8 -24.9 73.1 -20.5 -0.3 -4 ATCTGCTGAAATTCGGCCTC

1795 SEQ ID N0:5818 -3.8 -24.8 69.9 -19 -2 -10.2 TTTGTTGTTTCCTTCAAGGT

2003 SEQ ID N0:5819 -3.8 -23.1 70 -18.5 -0.6 -5.2 GTGAGTCTCTCCTGCTTTCG

2283 SEQ ID N0:5820 -3.8 -27 79.2 -22.6 -0.3 -4.3 CCTGGCACCATCCTGGATGA

2362 SEQ ID N0:5821 -3.8 -29.2 78.2 -23.5 -1.2 -11.8 CCGTTTCAGTGGTGGTGCCC

2490 SEQ ID N0:5822 -3.8 -31.1 84.8 -26.7 -0.3 -5.1 CGGACATTTCTGTCTAGATT

2633 SEQ ID N0:5823 -3.8 -21.9 65.6 -15.4 -2.7 -8.7 GGCAAATCTATACCAGCATG

2654 SEQ ID N0:5824 -3.8 -22.4 64.6 -17.9 -0.5 -4.3 AGCACGCGGAACACAATCAG

3271 SEQ ID N0:5825 -3.8 -23.4 64.7 -19.1 0 -7.9 GAACTCAATAATAAGGCCAG

3409 SEQ ID N0:5826 -3.8 -19.1 57.2 -14.8 0 -7.7 CATGCAGCTGTCTATCTTAT

3599 SEQ ID N0:5827 -3.8 -23.2 70 -18.7 0 -8.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GCTTCCGGTTTAAAGTCTTC

3952 SEQ ID N0:5828 -3.8 -23.9 70.1 -20.1 0 -6.6 ATGAACACAATGAAAGTCTC

4096 SEQ ID N0:5829 -3.8 -17.1 53.9 -13.3 0 -3.1 TACCAGGCTAACCAAAGAAA

4316 , SEQ ID N0:5830-3.8 -20.2 58.2 -15.7 -0.5 -4.3 AGCCAAGAGCATTGGCTACC

4332 SEQ ID N0:5831 -3.8 -26.8 74.5 -17.8 -5.2 -14.3 TTCATGATAGAGGGAATTGC

4462 SEQ ID N0:5832 -3.8 -20.3 61.8 -16.5 0 -6.4 TTGTCATGTTAACACAGTGG

4557 SEQ ID N0:5833 -3.8 -20.9 63.8 -16.3 -0.5 -8.7 AATACATGTACAGATTTTCT

4788 SEQ ID N0:5834 -3.8 -17.9 56.9 -13.6 0 -8.2 GATTCGGCCAATCCTGGCAA

5345 SEQ ID N0:5835 -3.8 -27.2 72.8 -19.1 -4.3 -10.3 AACAACGCAGGAAGGGACAT

5419 SEQ ID N0:5836 -3.8 -22 62.1 -18.2 0 -3.6 AGGAAGGATATGATGATGTA

5731 SEQ ID N0:5837 -3.8 -18.7 58.1 -14.9 0 -2.6 CACTGAAGTTCTCCAGGATG

5778 SEQ ID N0:5838 -3.8 -23.2 68.1 -18.6 -0.6 -6.3 GCAAATAAAATATCAAGACA

6016 SEQ ID N0:5839 -3.8 -14.3 47.5 -10.5 0 -3.4 ACTCTCACACAAAACACGCT

6044 SEQ ID N0:5840 -3.8 -21.8 62.3 -l8 0 -3.3 ATAACATCTGAAATTACGCT

6194 SEQ ID N0:5841 -3.8 -17.9 54.8 -14.1 0 -3.3 TTTAACCTTTGCTTTAAAAG

6214 SEQ ID N0:5842 -3.8 -17 53.5 -12.7 -0.2 -4.3 CATAGGCTGTAAACAATTGA

6501 SEQ ID N0:5843 -3.8 -18.7 57 -14.9 0 -7.2 CTCAGCAGTGTCAGCTGGTA

6689 SEQ ID N0:5844 -3.8 -26.8 80.4 -20.2 -2.8 -10 GAAAATGGAAGTAACTGAAA

7035 SEQ ID N0:5845 -3.8 -14.1 47.1 -10.3 0 -3 TTGATTATATTTTTACATTA

7850 SEQ ID N0:5846 -3.8 -15 50.7 -11.2 0 -2.2 TTCTTATGTACAAAATGCTG

7880 SEQ ID N0:5847 -3.8 -17.6 55.3 -13.8 0 -6.8 AAAAAATGAAATTTATTCTT

8459 SEQ 2D N0:5848 -3.8 -11.1 41.7 -6 -1.2 -5.2 GATAGCATCAATTATATTGA

8811 SEQ ID N0:5849 -3.8 -17.4 55.3 -11.8 -1.8 -7.5 AATTCTCAATAACTATATCA

8917 SEQ ID N0:5850 -3.8 -15.7 51.5 -11.9 0 -2.4 TTGTCTTTAAAAATATGCAT

9021 SEQ ID N0:5851 -3.8 -15.9 51.5 -12.1 0 -6.6 GAAATACATATTTAGATCCA

9043 SEQ ID N0:5852 -3.8 -17.1 53.9 -12.7 -0.3 -5.8 CCACAGAGGTTTACAAATTA

88 SEQ ID N0:5853 -3.7 -19.8 59.5 -16.1 0 -3.2 GCATAACAAAGCCCAGCATC

177 SEQ ID N0:5854 -3.7 -24.4 67.7 -19.8 -0.7 -4.9 GCCACTCTACTATGAATCCT

291 SEQ ID N0:5855 -3.7 -24.6 70.2 -20.9 0 -2.2 ATCCCTAGAAGAGATTCTTT

327 SEQ ID N0:5856 -3.7 -21.7 65 -15.7 -2.3 -4.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GGCACTGAATCGGAAAATTG

764 SEQ ID N0:5857 -3.7 -19.7 57.6 -16 0 -4.3 TGGCACTGAATCGGAAAATT

765 SEQ ID N0:5858 -3.7 -19.7 57.6 -16 0 -4 ACCTGGAATGACTGAAATTG

1148 SEQ TD N0:5859 -3.7 -19.2 57.2 -15.5 0 -5.l CCATGGAGAAAAGGAAGCTG

2076 SEQ ID N0:5860 -3.7 -20.8 60.2 -17.1 0 -7.3 ACTCAAGAGAGACTGATGAG

2135 SEQ ID N0:5861 -3.7 -19.6 60.5 -14.3 -1.5 -6.3 TTTCAGATCCAACATCCTTT

2223 SEQ ID N0:5862 -3.7 -22.7 66.5 -19 0.7 -3.7 TCTTCCCATTTGCTGGAAGC

2382 SEQ ID N0:5863 -3.7 -26.3 74.4 -19.9 -2.7 -8 ACACCATTGCAATCCACAGT

2410 SEQ ID N0:5864 -3.7 -24.9 69.8 -20.6 0 -8.4 ACCACACCATTGCAATCCAC

2413 SEQ ID N0:5865 -3.7 -25.9 70.4 -21.6 0 -8.6 GTGGTGCCCTCTGGGGGAAG

2479 SEQ ID N0:5866 -3.7 -30.3 83.3 -24.4 -2.2 -8.7 GTAAGAGCTTAACCTTCTCT

2519 SEQ ID N0:5867 -3.7 -22.4 67.2 -17.8 -0.8 -4.9 AATGCTGGCTATGCTCACGG

2585 SEQ ID N0:5868 -3.7 -25.6 71.4 -21.2 -0.4 -5.9 TTCTTCCATTGTGTTGGTCA

2606 SEQ ID N0:5869 -3.7 -24.7 74.3 -21 0.2 -3.7 ACAGACCAAGCTCCATTAAA

2976 SEQ ID N0:5870 -3.7 -21.9 62.8 -18.2 0 -5 TTAATTAGCATATTTAGTGT

3070 SEQ ID N0:5871 -3.7 -17.4 56.3 -13.7 0 -6.1 CCCACATGGTCTCTATCCAC

3300 SEQ ID N0:5872 -3.7 -27.6 77.1 -23 -0.7 -5.2 GTCATCATCAGTAGCAGCAA

3446 SEQ ID N0:5873 -3.7 -23.7 71.3 -20 0 -5.4 AGATGAGCTGGTTGCATTTA

3869 SEQ ID N0:5874 -3.7 -22.8 68.4 -17.5 -1.5 -5.1 AGTCTTCTTCGGGTTCAGTT

3939 SEQ ID N0:5875 -3.7 -25.3 77.1 -21.6 0 -3 TGTCCGTAATGATTTGATGG

4367 SEQ ID N0:5876 -3.7 -21.2 62.4 -17.5 0 -3.2 CAACAAGAGCATTCACAACC

4440 SEQ ID N0:5877 -3.7 -20.9 60.8 -16.6 -0.3 -4.l GCAAACAAATTCACACCCAT

4525 SEQ ID N0:5878 -3.7 -21.6 60.8 -17.9 0 -3.4 GTCACTCAAATTGTTAACAT

4601 SEQ ID N0:5879 -3.7 -18.4 57.3 -14.2 0 -8.3 ACCCAAAGATGATAAAGATG

4818 SEQ ID N0:5880 -3.7 -17.6 53.5 -13.9 0 -2.1 TAGGATTCGGCCAATCCTGG

5348 SEQ ID N0:5881 -3.7 -26.3 72.1 -18.7 -3.5 -15.6 TTCCAGCTTCCTTTTTAACA

5508 SEQ ID N0:5882 -3.7 -23.6 68.8 -19.9 0 -4.5 GTTGTAATTTGGAACAAGCA

5572 SEQ ID N0:5883 -3.7 -19.5 59.2 -14.2 -1.6 -7.3 AAAAGAAAATCCCAACAGAT

5703 SEQ ID N0:5884 -3.7 -16.3 50.5 -12.6 0 -2.9 GGCTCTGCACTTTCTTCAGT

5803 SEQ ID N0:5885 -3.7 -26.7 79.8 -22.1 -0.7 -6.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCAAACTTTTCCCAAACCTC

5854 SEQ ID N0:5886 -3.7 -22.3 63.3 -18.6 0 -2.5 TTTGTTACACTATCATAGGA

6376 SEQ ID N0:5887 -3.7 -19.5 61 -15.8 0 -3.3 CTTTCTCAAACTTTTCCTTG

6402 SEQ ID N0:5888 -3.7 -20.9 63.1 -I7.2 0 -2.5 TTTCCTTTGCTTTCTTTTTC

6430 SEQ ID N0:5889 -3.7 -22.7 69.7 -19 0 -3.6 CAGCAGTGTCAGCTGGTAAT

6687 SEQ ID N0:5890 -3.7 -24.8 73.5 -I8.9 -2.2 -9.2 TCAGATAGAAACAAACATAA

7089 SEQ ID N0:5891 -3.7 -14 47.1 -10.3 0 -2.4 AACTATTCTAAAGCAATACT

7282 SEQ ID N0:5892 -3.7 -16.4 52.5 -12.7 0 -4.1 CTGCAGCAGAAAGTGGAACA

7301 SEQ ID N0:5893 -3.7 -22.3 64.5 -17.2 -0.9 -10.4 GGAATTGTGATTAAAAATCA

7954 SEQ ID N0:5894 -3.7 -15.1 49.4 -9.6 -1.8 -6.9 ATTGGAATGGTAGAAAATAT

8061 SEQ ID N0:5895 -3.7 -15.5 50.4 -11.8 0 -2.4 TACTACTATTATTATCAAAC

8725 SEQ ID N0:5896 -3.7 -14.8 49.8 -11.1 0 -1.3 AAATCCAAGGGTTCTTGAAA

8952 SEQ ID N0:5897 -3.7 -19.3 57.8 -14 -1.6 -6.7 ATAAATCCAAGGGTTCTTGA

8953 SEQ ID N0:5898 -3.7 -20.4 61.1 -14.6 -2.1 -6.7 CCTAGAAGAGATTCTTTGCT

324 SEQ ID N0:5899 -3.6 -22 65.8 -16.1 -2.3 -8.3 GAATTACCTTAGATAGTCAC

409 SEQ ID N0:5900 -3.6 -19.4 60.2 -15.8 0 -3.2 GATAGCAATTTTCCTAACAG

818 SEQ ID N0:5901 -3.6 -19.6 59.5 -16 0 -4.1 CTGAAATCCAGCCAGTTCCA

1039 5EQ ID N0:5902 -3.6 -26.1 72.1 -22 -0.2 -4.9 TCTGTCACATATGCCATCAC

1066 SEQ ID N0:5903 -3.6 -24 70.5 -20.4 0 -6.5 TAATCCTTCCAGTTAAATGT

1405 SEQ ID N0:5904 -3.6 -20.3 60.9 -16.7 0 -5.6 AAAGCTTGTGTAGCCATAGT

1550 SEQ ID N0:5905 -3.6 -22.9 67.9 -17.7 -1.5 -7.9 TCAAATTCACCAAATAAAAT

1707 SEQ ID N0:5906 -3.6 -14 46.6 -10.4 0 -3.1 CAGGATCAAATTCACCAAAT ' 1712 SEQ ID N0:5907 -3.6 -18.9 56.5 -14.7 -0.3 -5.3 TGCTGAAATTCGGCCTCTTT

1792 SEQ ID N0:5908 -3.6 -24.6 69.1 -19 -2 -10.2 TGTTTCCTTCAAGGTGCTCT

1998 SEQ ID N0:5909 -3.6 -25.6 75.7 -22 0 -5.3 GCTGTCTCTCTCTCCTTTGT

2018 SEQ ID N0:5910 -3.6 -27.8 84.3 -24.2 p -2,g CTGAAAATGCTTGTTTTGCT

2188 SEQ ID N0:5911 -3.6 -20.4 61 -15.9 -0.8 -4.8 TAAGAGCTTAACCTTCTCTT

2518 SEQ ID N0:5912 -3.6 -21.3 64.3 -16.1 -I.6 -5.7 TAACAATTAAATTCACAAGA

2724 SEQ ID N0:5913 -3.6 -13.6 46.4 -10 0 -3.1 TCAACACACTACTGAATTGC

.2829 SEQ ID N0:5914 -3.6 -19.9 59.8 -15.8 -0.1 -3.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CCTACAGTCAACACACTACT

2836 SEQ ID N0:5915 -3.6 -22.8 66.5 -18.7 -0.1 -2.9 GAACCATTTCTGCTGTGAAA

2877 SEQ ID N0:5916 -3.6 -21.4 62.6 -17.1 -0.5 -4.6 TTCTTGGAAATAGTAATAAG

2918 SEQ ID N0:5917 -3.6 -14.8 49.6 -11.2 0 -3.1 ATTAAACTGAGGCTGACAAT

2962 SEQ ID N0:5918 -3.6 -18.8 57.1 -15.2 0 -3.7 AACTCAATAATAAGGCCAGA

3408 SEQ ID N0:5919 -3.6 -19.1 57.2 -15 0 -7.7 TCGATTACGTATTTTTCAAC

3709 SEQ ID N0:5920 -3.6 -18.5 56.9 -14 -0.7 -5.8 TTTATGAATGACATATAATC

3736 SEQ ID N0:5921 -3.6 -14.4 48.7 -9.7 -1 -4 AGCAATTGGCACTGTGACGG

3770 SEQ ID N0:5922 -3.6 -24.7 69.4 -19.5 -1.5 -7.1 CTTTCTTCTAGTTCTGACTC

3838 SEQ ID N0:5923 -3.6 -22.2 70 -17.9 -0.4 -4 GTTGCATTTAATTTCTCTTT

3859 SEQ ID N0:5924 -3.6 -20 62.6 -16.4 0 -5.1 GCCAAGAGCATTGGCTACCA

4331 SEQ ID N0:5925 -3.6 -27.5 75.3 -19.5 -4.4 -12.8 TCTTCATATACAGGCTGAAG

4771 SEQ ID N0:5926 -3.6 -20.6 62.9 -15.3 -1.7 -6.5 GTTGAAGTTATCTATGATGA

4868 SEQ ID N0:5927 -3.6 -18.4 58.4 -14.8 0 -3.2 GATGTACATGTTCACCACAA

5753 SEQ ID N0:5928 -3.6 -21.9 64.5 -17.6 -0.4 -8.4 TCTCCAGGATGACCGCGATG

5769 SEQ ID N0:5929 -3.6 -27 72.5 -22 -1.3 -7.2 AAACCTCATAGAACATCTCA

5841 5EQ ID N0:5930 -3.6 -19.7 59.3 -16.1 0 -2.8 CAGTGGATCCGGTCACCACT

5998 SEQ ID N0:5931 -3.6 -28.4 77.9 -21.7 -3 -13.5 CATCTCTCCACTCTCACACA

6053 SEQ ID N0:5932 -3.6 -25.7 74.8 -22.1 0 -0.5 ACTTTGGAGGGGTTTGATGC

6109 SEQ ID N0:5933 -3.6 -24.5 72.2 -20.9 0 -2.6 CCTCCCTTTAATTGCCTCTT

6257 SEQ ID N0:5934 -3.6 -27.6 75.8 -24 0 -3 CTGACTATGAGTATTTGTTA

6581 SEQ ID N0:5935 -3.6 -19 60.1 -14.9 -0.1 -3 CACAACTATCCCTATAGTCT

6729 SEQ ID N0:5936 -3.6 -22.9 67 -17.3 -2 -5.4 CCATGGAAATGCACTAGAAC

6845 SEQ ID N0:5937 -3.6 -20.5 59.7 -15.5 -1.3 -7.3 AATACTTTCAAAATTAAAGC

7061 SEQ TD N0:5938 -3.6 -13.5 46.4 -9 -0.7 -5.6 GGTGTTTGTAAATAGCTTCA

7688 SEQ ID N0:5939 -3.6 -20.9 64.1 -17.3 0 -4.9 AAATAAAGTTGATTATATTT

7858 SEQ ID N0:5940 -3.6 -12.5 44.7 -8.9 0 -3.2 TTTGGAATTGTGATTAAAAA

7957 SEQ ID N0:5941 -3.6 -14.2 47.7 -10.6 0 -3 ATACGTAAATACTACAAAAG

8180 SEQ ID N0:5942 -3.6 -13.5 46 -9.2 -0.4 -6.6 AAAAAGAACAAAGTCCATGT

8254 SEQ ID N0:5943 -3.6 -16.1 50.8 -12.5 0 -4.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAAAATGAAATTTATTCTTT

8458 SEQ ID N0:5944 -3.6 -11.9 43.3 -7 -1.2 -5.2 CACAGGTTTTATTAATTATA

8548 SEQ ID N0:5945 -3.6 -16.6 53.9 -13 0 -4.2 CAAATTAGTTACAATTTTTT

75 SEQ ID N0:5946 -3.5 -14.8 49,5 -10.6 -0.5 -4 TTGCTCCATAATCTCTGATA

219 SEQ ID N0:5947 -3.5 -21.8 65.2 -18.3 0 -3.6 TACCAACAGTGCCTGTGCCA

473 SEQ ID N0:5948 -3.5 -28.4 77 -23.8 -1 -8.2 CACCATCTCTGGAGGAATGT

671 SEQ ID N0:5949 -3.5 -24,3 70.1 -19.2 -1.5 -6.1 ACTATAAAAGTTTTCTTATT

727 SEQ ID N0:5950 -3.5 -15.1 50.7 -10.5 -1 -6 CCACAAACTCTGTCACATAT

1074 SEQ ID N0:5951 -3.5 -21.5 63 -17.5 -0.1 -4.2 CAGACAGAACACAGTCAGGA

1226 SEQ ID N0:5952 -3.5 -22 65.4 -17 -1.4 -4.6 CAAACACGCTCAGACAGAAC

1236 SEQ ID N0:5953 -3.5 -20.6 59.8 -17.1 0 -3.4 TCCAATGTAATCCTTCCAGT

1412 SEQ ID N0:5954 -3.5 -24.3 69.6 -20.8 0 -2.2 ATAAAAGTGACTGTCATCTC

1430 SEQ ID N0:5955 -3.5 -18.5 58.3 -14.3 -0.1 -8.8 AGCATCTGCTGAAATTCGGC

1798 SEQ ID N0:5956 -3.5 -24 68.4 -18.9 -1.6 -9.8 TGAGTCTCTCCTGCTTTCGC

2282 SEQ ID N0:5957 -3.5 -27.6 80.1 -23.4 -0.4 -4.5 TCTTGAGAACCATTTCTGCT

2883 SEQ ID N0:5958 -3.5 -23 67.7 -18.6 -0.7 -5.2 TGGGCCAGGATTTTGCCAAC

3051 SEQ ID N0:5959 -3.5 -27 74 -21.2 -2.3 -7.9 ATATTTAGTGTGGGCCAGGA

3061 SEQ ID N0:5960 -3.5 -24.7 72.3 -20.7 0 -7.6 CACTCTCCACACAGCACGCG

3283 SEQ ID N0:5961 -3.5 -28.1 75 -24.6 0 -7.4 CAGCGACCTCCATACAGTCC

3318 SEQ ID N0:5962 -3.5 -28.3 77.1 -24.3 -0.1 -3.5 CAGATTATTCATTTCATTGT

3464 SEQ ID N0:5963 -3.5 -18.9 59.7 -14.9 -0.2 -3 TTTTGCATTCTTCCTACTGC

3490 SEQ ID N0:5964 -3.5 -24.2 71.7 -20.1 -0.3 -5.1 AAAGGCTTTTTGGAAACACT

3542 SEQ ID N0:5965 -3.5 -19.3 58 -15 -0.6 -7.3 TTTCTTCTAGTTCTGACTCA

3837 SEQ ID N0:5966 -3.5 -22 69.1 -17.8 -0.4 -4 CAGTTGTGCTCAACAATACT

4072 SEQ ID N0:5967 -3.5 -21.3 63.8 -14.5 -3.3 -6.6 GCATTAGTGAAATATGTTTG

4264 SEQ ID N0:5968 -3.5 -17.8 56.2 -14.3 0 -4.1 CCTTTAAATGTGGCCACTTG

4705 SEQ ID N0:5969 -3.5 -23.5 66.5 -17.6 0 -l3 ATGACAAAGTATAAATACAT

4801 SEQ ID N0:5970 -3.5 -13.9 47.1 -9.7 -0.5 -5.8 AAGTTATCTATGATGACACC

4864 SEQ ID N0:5971 -3.5 -19.6 60 -14.6 -1.4 -5.8 ATCAAAGACTTGTCTGGTTA

5039 SEQ ID N0:5972 -3.5 -20.2 62.3 -15.7 -0,9 -6.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ACGCAGGAAGGGACATCATC

5415 SEQ ID N0:5973 -3.5 -24 68.4 -20.5 0 -3.6 GGGTCACAGTCGGGTGGTGC

5635 SEQ ID N0:5974 -3.5 -29.8 85.4 -25.5 -0.6 -5.6 CCAGGAAGGATATGATGATG

5733 SEQ ID N0:5975 -3.5 -20.5 60.6 -l7 0 -3.1 ACTCTATAAACTGGGTCGCA

5880 SEQ ID N0:5976 -3.5 -22.8 66 -19.3 0 -3.6 CAAAATCAGAGAGTTTAGAG

5901 SEQ ID N0:5977 -3.5 -16.6 53.6 -12.6 -0.1 -4.4 CTGCAAAATCAGAGAGTTTA

5904 SEQ ID N0:5978 -3.5 -18.7 57.8 -14.7 -0.1 -4.9 ACAGTGGATCCGGTCACCAC

5999 SEQ ID N0:5979 -3.5 -27.7 76.5 -21.9 -2.2 -12 ATGCAAGGACTGTACTAAAG

6779 SEQ TD N0:5980 -3.5 -19 58 -15.5 0 -5.6 ACCTTTGTCTGATCATTTGA

6955 SEQ ID N0:5981 -3.5 -22.3 67 -18.8 0 -6.2 CATAAAAATAATCCTTTAAA

7141 SEQ ID N0:5982 -3.5 -12.4 43.9 -8.9 0 -4 CAACTATTCTAAAGCAATAC

7283 SEQ ID N0:5983 -3.5 -16.2 51.9 -12.7 0 -4.1 GAATTGTGATTAAAAATCAA

7953 SEQ ID N0:5984 -3.5 -13.2 45.6 -7.7 -2 -8.2 ACAGGTTTTATTAATTATAT

8547 5EQ ID N0:5985 -3.5 -15.9 52.5 -12.4 0 -4.2 TAGCCAAGGGAGTTAATTGA

8768 SEQ ID N0:5986 -3.5 -21.5 63.6 -18 0 -3.7 AACATTTATTAGCCACCAAG

8840 SEQ ID N0:5987 -3.5 -20.6 60.4 -17.1 0 -3.2 ACCTCACATAAATCCAAGGG

8960 SEQ ID N0:5988 -3.5 -22.2 63.3 -18.1 -0.3 -3.9 TATGAATCCTTGACAGGTAT

281 SEQ ID N0:5989 -3.4 -20.6 62.1 -16.2 -0.9 -3.4 ACTATGAATCCTTGACAGGT

283 SEQ ID N0:5990 -3.4 -22 65.1 -17.6 -0.9 -3.4 CTTCTTGGCTTTCTCTTCTG

560 SEQ ID N0:5991 -3.4 -24.5 74.6 -21.1 0 -3.7 TTCCAGCTTCCAAGTCACTA

627 SEQ ID N0:5992 -3.4 -25.5 73.8 -22.1 0 -4.5 GGAAGGTTCTTTCCAGCTTC

637 SEQ ID N0:5993 -3.4 -25.4 75.3 -20 -2 -8.6 ACAAGGCAGAGGTGGCACTG

777 SEQ ID N0:5994 -3.4 -25.7 73.4 -21.7 -0.3 -4.5 ATGACACTGAAATCCAGCCA

1045 SEQ ID N0:5995 -3.4 -23.4 65.7 -18.8 -1.1 -4.9 GCCCAGGTCCACAAACTCTG

1082 SEQ ID N0:5996 -3.4 -27.9 75.7 -24.5 0 -3.4 GAATGTTCTCAACGCTGAGA

1106 SEQ ID N0:5997 -3.4 -21.8 64 -15.8 -2.6 -8 CTTTACCGACTGGATCAGGG

1187 SEQ ID N0:5998 -3.4 -24.8 69.9 -20.5 -0.7 -5.3 CAGAACACAGTCAGGATCAT

1222 SEQ ID N0:5999 -3.4 -21.6 64.7 -18.2 0 -5.3 CTTTTTGCCCATCCAAAACA

1449 SEQ ID N0:6000 -3.4 -23.7 65.4 -18.8 -1.4 -5.4 GAAATAGAGACAGGAAAGCC

1584 5EQ ID N0:6001 -3.4 -19.2 57.5 -15.8 0 -3.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CATGGAGAAAAGGAAGCTGC

2075 SEQ ID N0:6002 -3.4 -20.6 60.4 -17.2 0 -5.2 TAAGACAATGCAAATAGTGA

2768 SEQ ID NO:6003 -3.4 -16.4 52.1 -12.1 -0.7 -5.6 CCTTCTTGGAAATAGTAATA

2920 5EQ ID N0:6004 -3.4 -18.4 56.8 -15 0 -3.2 CCATTAAACTGAGGCTGACA

2964 SEQ TD N0:6005 -3.4 -22.2 63.8 -18.8 0 -3.7 CTTAATTAGCATATTTAGTG

3071 SEQ ID N0:6006 -3.4 -17.1 55.3 -13.7 0 -5 TCCACACAGCACGCGGAACA

3278 SEQ ID NO:6007 -3.4 -27 71.3 -23.1 0 -7.9 ATTTTTCACATAATCAATTC

3512 SEQ ID N0:6008 -3.4 -16.3 53.2 -12.9 0 -2.5 CATCTTATTTTTCACATAAT

3518 SEQ ID N0:6009 -3.4 -17.2 55.1 -13.8 0 -2.2 GCACTGTGACGGTGAGGCTG

3762 SEQ ID N0:6010 -3.4 -27.3 76.6 -22.1 -1.8 -7.6 AAAACAAGCTTCCGGTTTAA

3959 SEQ ID N0:6011 -3.4 -19.7 57.7 -15.4 -0.7 -8.9 TAAAACAAGCTTCCGGTTTA

3960 SEQ TD N0:6012 -3.4 -20.1 59 -15.8 -0.7 -8.9 TCATGATAGAGGGAATTGCT

4461 SEQ ID NO:6013 -3.4 -21.1 63.4 -17.7 0 -5.9 CACAGTGGTAGAACTTGCCA

4545 SEQ ID NO:6014 -3.4 -24.5 70.4 -20.4 -0.5 -5.2 TGACAGTCACTCAAATTGTT

4606 SEQ ID NO:6015 -3.4 -20 61.3 -16.6 0 -7 TGAACACTAGGTTGATCCGG

5148 SEQ ID N0:6016 -3.4 -23.1 66 -18.5 -1.1 -6.8 GGGGACACAGAATACTTTTC

5302 SEQ ID NO:6017 -3.4 -21.4 64.1 -18 0 -3.5 AGAAAATCCCAACAGATGGG

5700 SEQ ID N0:6018 -3.4 -20.8 59.7 -14.5 -2.9 -7.4 GATCCGGTCACCACTGACCA

5993 SEQ ID NO:6019 -3.4 -28.8 77 -21.5 -3.9 -11.8 TTTGGAGGGGTTTGATGCCA

6107 SEQ ID N0:6020 -3.4 -26.1 74.4 -21.6 -1 -4.7 ATCTGAAATTACGCTGAATG

6189 SEQ ID NO:6021 -3.4 -17.9 54.7 -14.5 0 -3.3 GATATATTTTTTAACCTTTG

6223 SEQ ID N0:6022 -3.4 -16.8 54 -13.4 0 -4.3 CCTTCATAGGCTGTAAACAA

6505 SEQ ID N0:6023 -3.4 -21.4 62.6 -17.5 -0.2 -5.7 ACAGAGTTGCAGTGACAGAG

6628 SEQ ID N0:6024 -3.4 -22.6 68.5 -19.2 0 -4.9 CTGTAGCCATTACAATTCTT

7015 SEQ ID N0:6025 -3.4 -21.8 65 -17.4 -0.9 -3.9 TTTGCACTTGCTGAAACAGA

7169 SEQ ID N0:6026 -3.4 -21.3 62.8 -17.2 -0.5 -6.5 AGAGCAGAAGATGGCAAAGC

7325 SEQ ID N0:6027 -3.4 -21.7 63.7 -17.4 -0.8 -4.7 TTTAATGAATGATACAGGAT

7514 SEQ ID N0:6028 -3.4 -16.1 51.9 -12.7 0 -2.5 CCTTTAAACTATGTTTAATG

7527 SEQ ID NO:6029 -3.4 -16.6 52.9 -11.1 -2.1 -8 CAAATAAAGTTGATTATATT

7859 SEQ ID N0:6030 -3.4 -13.1 45.7 -9,7 0 -2.9 kcal/

mol kcal/moldeg kcal/molkoal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AATGCTGACAAATAAAGTTG

7867 SEQ ID N0:6031 -3.4 -15.7 50.5 -12.3 0 -3.6 TCTATGAATAAAAGGTTATA

8143 SEQ ID N0:6032 -3.4 -14.4 48.6 -10.4 -0.3 -3.1 GTCTGCATACGTAAATACTA

8186 SEQ ID N0:6033 -3.4 -19.7 59.6 -16.3 0 -6.6 AAAATGAAATTTATTCTTTT

8457 SEQ ID N0:6034 -3.4 -12.7 45 -7.8 -1.4 -6.5 TGCATTTAAGTAACAAAGAA

8863 SEQ ID N0:6035 -3.4 -15.3 49.9 -11.9 0 -4.7 AAGATGGTTAGGGTATAACG

156 SEQ ID N0:6036 -3.3 -19.4 58.8 -14.1 -2 -4.6 TCCAAGATGGTTAGGGTATA

159 SEQ ID N0:6037 -3.3 -22.2 66.4 -18 -0.8 -4.9 CATCCAAGATGGTTAGGGTA

161 SEQ ID N0:6038 -3.3 -23.2 68.2 -19 -0.8 -4.9 CCCTAGAAGAGATTCTTTGC

325 SEQ ID N0:6039 -3.3 -23.1 67.6 -17.5 -2.3 -7.5 CCTTTTTCCGGAAAAGCTCC

385 SEQ ID N0:6040 -3.3 -25.3 68.9 -18.8 -2.1 -14.4 AAAAGGCGGAAGCTTTCAGG

502 SEQ ID N0:6041 -3.3 -22 62.9 -16.6 -2.1 -7.6 GGCTCTGACACCATCTCTGG

679 SEQ ID N0:6042 -3.3 -27.4 78.1 -22.9 -1.1 -5.9 AGTGCTCGGAGAACTCTGAA

1123 SEQ ID N0:6043 -3.3 -23.1 67.2 -19 -0.6 -8 TCCTCAGATTGCCCATGAAC

1275 SEQ ID N0:6044 -3.3 -25.7 71.5 -22.4 0 -4.5 AAAATGACCAGGACAAAAAA

1678 SEQ ID N0:6045 -3.3 -14.6 47.2 -11.3 0 -4 TGGAGAAAAGGAAGCTGCTT

2073 SEQ ID N0:6046 -3.3 -20.9 61.4 -16.8 0 -9.4 TGATCTTGAGAACCATTTCT

2886 SEQ ID N0:6047 -3.3 -20.9 62.9 -16.4 -1.1 -7.1 GCCAGGATTTTGCCAACTTG

3048 SEQ ID N0:6048 -3.3 -25.6 71.3 -21.7 -0.3 -3.8 TGTGGGCCAGGATTTTGCCA

3053 SEQ ID N0:6049 -3.3 -28.7 79.1 -23.1 -2.3 -7.9 AAGAAGTCGTTCATGTGCCA

3241 SEQ ID N0:6050 -3.3 -23.7 68.4 -19.1 -1.2 -4.7 GGTCTCTATCCACTCTCCAC

3293 SEQ ID N0:6051 -3.3 -27.5 80.3 -24.2 0 -2.8 CACATGGTCTCTATCCACTC

3298 SEQ ID N0:6052 -3.3 -24.9 73.5 -20.7 -0.7 -5.2 CAATGACCATGACCAACATG

3363 SEQ ID N0:6053 -3.3 -21.2 60.2 -16 -1.9 -5.4 CATTAGTGAAATATGTTTGA

4263 SEQ ID N0:6054 -3.3 -16.6 53.5 -13.3 0 -4.1 GTTGATCCGGGACAAAACTA

5138 SEQ ID N0:6055 -3.3 -21.7 61.9 -17.7 0 -8.7 ACACTAGGTTGATCCGGGAC

5145 SEQ ID N0:6056 -3.3 -25.2 71.4 -20.7 -1.1 -6.8 ATGAACACTAGGTTGATCCG

5149 SEQ ID N0:6057 -3.3 -21.9 63.6 -17.4 -1.1 -6 AGGTTGTAATTTGGAACAAG

5574 SEQ ID N0:6058 -3.3 -18.2 56.7 -13.3 -1.6 -7.3 AAGAAAATCCCAACAGATGG

5701 SEQ ID N0:6059 -3.3 -18.9 55.8 -14.5 -1 -4.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GAACATCTCAAAGTCATCCT

5831 SEQ ID N0:6060 -3.3 -21.4 63.6 -18.1 0 -2.8 CCACTGACCATGGGCAGATC

5983 SEQ ID N0:6061 -3.3 -27.2 75 -22.1 -1.8 -8.8 ACGCTGAATGATAGCGGCAG

6179 SEQ ID N0:6062 -3.3 -24.2 67.1 -17.3 -3.6 -10.6 CTTTAAAAGATAACATCTGA

6203 SEQ ID N0:6063 -3.3 -15.1 49.7 -10.9 -0.8 -6.5 ACTTGATATATTTTTTAACC

6227 SEQ ID N0:6064 -3.3 -16.9 54.2 -13.6 0 -3.7 GGAACTCCCATCTGTTTTTT

6338 SEQ ID N0:6065 -3.3 -24.3 70.4 -20.5 -0.1 -3.9 AAAACAGTCAGTTTGGCATG

6562 SEQ ID N0:6066 -3.3 -20.2 60.9 -15.3 -1.6 -7.7 AAGGACTGTACTAAAGGTGT

6775 SEQ ID N0:6067 -3.3 -20.1 61.3 -16.8 0 -4.8 AAAATACTTTCAAAATTAAA

7063 SEQ ID N0:6068 -3.3 -10.3 40.2 -7 0 -3.2 TTGCATAAAAATAATCCTTT

7144 SEQ ID N0:6069 -3.3 -16 50.9 -12.7 0 -5.1 TTTGCATAAAAATAATCCTT

7145 SEQ TD N0:6070 -3.3 -16 50.9 -12.7 0 -5.1 ACTGCAGCAGAAAGTGGAAC

7302 SEQ ID N0:6071 -3.3 -21.8 63.9 -17.2 -0.7 -10.4 ACATAACATAGGGTATTTAA

7370 SEQ ID N0:6072 -3.3 -17 54.1 -13.7 0 -2.9 AGAATGGATTGAGTAAAATA

7584 SEQ ID N0:6073 -3.3 -14.9 49.3 -11.6 0 -2.3 AAGGTGTTTGTAAATAGCTT

7690 SEQ ID N0:6074 -3.3 -19.1 59.5 -15.8 0 -4.9 AAAATATATTATAACAAAGA

8048 SEQ ID N0:6075 -3.3 -10.2 40 -6.9 0 -6.2 AAGACTAGTCTGCATACGTA

8193 SEQ ID N0:6076 -3.3 -21.3 64 -16.3 0 -11.5 TAAGACTAGTCTGCATACGT

8194 SEQ TD N0:6077 -3.3 -21.3 64 -16.3 0 -11.5 TGCAGTTCTTCAATACACTA

8384 SEQ ID N0:6078 -3.3 -21 64.1 -17.7 0 -4.7 CAATAGCAATTCCCTGCATG

8402 SEQ TD N0:6079 -3.3 -23.4 65.9 -19.1 -0.9 -4.9 AAACAAAAAACCCCAGAGCC

8497 SEQ ID N0:6080 -3.3 -21.3 58.5 -18 0 -3.2 TTTGTTGTAAAATTCATGTA ' 8598 SEQ ID N0:6081 -3.3 -16.4 53.4 -12.4 -0.4 -6.6 ATATAATTTATTATGGGAGA

9068 SEQ ID N0:6082 -3.3 -16 52.3 -11.9 -0.6 -4.9 ACCACAGAGGTTTACAAATT

89 SEQ ID N0:6083 -3.2 -20.3 60.6 -16.1 -0.9 -4.7 GCAAGAGATTCTCTAGTAAA

520 SEQ ID N0:6084 -3.2 -18.9 59.1 -14.4 -1.2 -9.3 CCTAACAGGGTTTAGTGGAG

8,06 SEQ ID N0:6085 -3.2 -23.3 68.6 -19.6 -0.1 -3.6 CTCAAGGTCATAAATACACA

895 SEQ ID N0:6086 -3.2 -18.6 57.2 -15.4 0 -2.8 ATTCTTTGTCCAGTCAGGAG

920 SEQ TD N0:6087 -3.2 -24 72.9 -19.4 -1.3 -4.4 GTAAAATCTTCTAAGCAAAA

1003 SEQ ID N0:6088 -3.2 -14.9 49.1 -11.7 0 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TATGCCATCACAATGACACT

1057 SEQ ID N0:6089 -3.2 -22.1 63.9 -18.4 -0.1 -4.2 TAAAATGAGCCCAAGAAAAT

1693 5EQ ID N0:6090 -3.2 -16.5 50.8 -13.3 0 -3.2 ATGGAGAAAAGGAAGCTGCT

2074 SEQ ID N0:6091 -3.2 -20.8 61.1 -17.6 0 -7.2 TGGTCAGTCTGTTTCCATCC

2094 5EQ ID N0:6092 -3.2 -26.9 79.8 -23.7 0 -4.2 GAGACTGATGAGGGGAGCAG

2127 SEQ ID N0:6093 -3.2 -24.2 70.8 -20.5 -O.l -4.1 CAGAATGCTGGCTATGCTCA

2588 SEQ ID N0:6094 -3.2 -24.7 71 -20.7 -0.6 -5.9 TACAGTCAACACACTACTGA

2834 SEQ ID N0:6095 -3.2 -20.5 62 -15.6 -1.7 -5.6 TTAGTGTGGGCCAGGATTTT

3057 SEQ ID N0:6096 -3.2 -25.2 73.8 -21.5 0 -7.6 GGGAGCGTACAGTCATCATT

3217 SEQ ID N0:6097 -3.2 -24.9 72.7 -21.7 0 -4.8 GAAGAAGTCGTTCATGTGCC

3242 SEQ ID N0:6098 -3.2 -23.6 68.5 -19.1 -1.2 -4.7 CCACACAGCACGCGGAACAC

3277 SEQ ID NO:6099 -3.2 -26.8 70.4 -23.1 0 -7.9 AGCGACCTCCATACAGTCCC

3317 SEQ ID N0:6100 -3.2 -29.6 79.4 -25.9 -O.l -3.5 TTCCTACTGCAATCTGCAGA

3480 SEQ ID N0:6101 -3.2 -24.4 70.5 -16.8 -4.4 -11.4 AAAAGGCTTTTTGGAAACAC

3543 SEQ ID N0:6102 -3.2 -17.7 54.4 -13.6 -0.7 -7.2 ACCACTGGTGGTTCCATTCC

3671 SEQ ID N0:6103 -3.2 -28.5 79.6 -23.4 -1.5 -11.7 TAGCCAAGAGCATTGGCTAC

4333 SEQ ID N0:6104 -3.2 -24.5 70.4 -15.6 -5.7 -15.4 CGAGTTCTGAGTAGCCAAGA

4344 SEQ ID N0:6105 -3.2 -23.9 69.4 -20.7 0 -3.2 ATCCAGCCTTTAAATGTGGC

4711 SEQ ID N0:6106 -3.2 -24.5 69.6 -20.5 -0.6 -7.4 AGAAACATACCTACAATGGA

5269 SEQ ID N0:6107 -3.2 -18.6 56.1 -15.4 0.2 -4 CCGCGATGTACATGTTCACC

5757 SEQ ID N0:6108 -3.2 -26.4 71.7 -22.7 0 -7.8 AAAGTCATCCTCACTCAGGG

5822 SEQ ID N0:6109 -3.2 -23.9 70 -19.8 -0.8 -3.4 ACTTTGTTGGGTTTTGCTAT

5947 SEQ ID N0:6110 -3.2 -22.9 69.3 -19.7 0 -3.6 ATCCGGTCACCACTGACCAT

5992 SEQ ID N0:6111 -3.2 -28.2 75.7 -20.6 -4.4 -9.6 AAGACAGTGGATCCGGTCAC

6002 SEQ ID N0:6112 -3.2 -24.7 70.5 -19.9 -1.1 -10.8 CCCTTTAATTGCCTCTTTGT

6254 SEQ ID N0:6113 -3.2 -25.6 72.3 -22.4 0 -3 TCCCTTTAATTGCCTCTTTG

6255 SEQ ID N0:6114 -3.2 -24.8 70.6 -21.6 0 -3 TTTTTCTGGAGTGGAGTTCC

6323 SEQ ID N0:6115 -3.2 -24.2 73.3 -20.5 -0.1 -4 TAATTCTTTGTTTCTTTTTA

6472 SEQ ID N0:6116 -3.2 -17.4 57 -14.2 0 -2.9 TCAGCAGTGTCAGCTGGTAA

6688 SEQ ID N0:6117 -3.2 -25.2 75.4 -19.2 -2.8 -10 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ACTTTCAAAATTAAAGCCAT

7058 SEQ ID N0:6118 -3.2 -17.2 53.2 -13.1 -0.7 -5.6 TGAGAGCAGAAGATGGCAAA

7327 SEQ TD N0:6119 -3.2 -20.5 60.7 -17.3 0 -4.l CTGAGAGCAGAAGATGGCAA

7328 SEQ ID N0:6120 -3.2 -22.1 64.6 -18 -0.8 -5.2 ATATATTTGCCCAAACATGC

7413 SEQ TD N0:6121 -3.2 -21.4 61.7 -18.2 0 -5 AAACATCTATGAATAAAAGG

8148 SEQ ID N0:6122 -3.2 -13.2 45.5 -10 0 -3.8 GCAGTTCTTCAATACACTAC

8383 SEQ ID N0:6123 -3.2 -21.2 64.8 -18 0 -3.4 CATGCAGTTCTTCAATACAC

8386 SEQ ID N0:6124 -3.2 -21.1 63.9 -17.9 0 -5.5 CAATTCCCTGCATGCAGTTC

8396 SEQ TD N0:6125 -3.2 -26.1 73.7 -19.8 -0.2 -14.3 TATTTTAAACTGCCAACAGC

8526 SEQ ID N0:6126 -3.2 -19.9 59.2 -15.1 -l.5 -7.3 ACTACTATTATTATCAAACC

8724 SEQ ID N0:6127 -3.2 -17.1 54.2 -13.9 0 -1.3 CAGCATCCAAGATGGTTAGG

164 SEQ ID N0:6128 -3.1 -23.6 68.5 -19 -1.4 -6.2 TACAGCATAACAAAGCCCAG

l81 SEQ ID N0:6129 -3.1 -22.1 62.7 -18.5 -0.2 -4.l GGAGGAATGTCTCCATAAAT

661 SEQ ID N0:6130 -3.1 -20.8 61.7 -15.2 -2.5 -7 TTGCCAAGATTTTTATAAGT

978 SEQ ID N0:6131 -3.1 -18.8 58.1 -15.7 0 -4.1 GAACACAGTCAGGATCATCA

1220 SEQ ID N0:6132 -3.l -22 66 -18.9 0 -5.3 TTGGGGTTTCGACCAGCCTT

1531 SEQ ID N0:6133 -3.1 -28.6 78.4 -22.8 -2.7 -7.8 ATGAGCCCAAGAAAATGACC

1689 SEQ ID N0:6134 -3.1 -21.7 61 -18.6 0 -3.2 CAGCTGATGCTGCCGCAACT

1854 SEQ ID N0:6135 -3.1 -28.2 75.6 -22.4 -2.7 -12 CCAACAGCTCTCCTAACCCA

1899 SEQ ID N0:6136 -3.1 -28.1 74.9 -25 0 -4.2 CCACGGATACTCAAGAGAGA

2143 SEQ ID N0:6137 -3.1 -22.5 64.5 -17.8 -l.5 -6.2 TGACATACTGGCCTGACTAA

2345 SEQ ID N0:6138 -3.1 -22.9 65.9 -19.8 0 -7.2 ATGACATACTGGCCTGACTA

2346 SEQ ID N0:6139 -3.1 -23.6 68 -20.5 0 -7.2 TTCTGCTGTGAAAATCCCAG

2870 SEQ ID N0:6140 -3.1 -22.9 65.6 -19.8 0 -3.6 CATTAAACTGAGGCTGACAA

2963 SEQ ID NO:6141 -3.1 -19.5 58.3 -16.4 0 -3.7 CCAGGATTTTGCCAACTTGA

3047 SEQ ID N0:6142 -3.1 -24.4 68.5 -20.7 -0.3 -3.5 ATTGTCATCATCAGTAGCAG

3449 SEQ ID N0:6143 -3.1 -22 68.1 -18.9 0 -4.1 TCTTCTAGTTCTGACTCACT

3835 SEQ ID N0:6144 -3.1 -22.9 71.2 -19.1 -0.4 -4 AAACAAGCTTCCGGTTTAAA

3958 SEQ ID N0:6145 -3.1 -19.7 57.7 -16 -0.2 -8 TGAAATCCATAAGCAACCCA

4246 SEQ ID N0:6146 -3.1 -22 61.4 -18.9 0 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATTGGCTACCAGGCTAACCA

4322 SEQ ID N0:6147 -3.1 -26.4 72.9 -21.7 -1.5 -7.2 ATAAAGATGACAAAGTATAA

4807 SEQ ID N0:6148 -3.1 -12.9 45.2 -9.8 0 -2.8 ATAGTGAAGTAGTAGTGTCT

5212 SEQ ID N0:6149 -3.1 -20.3 65.2 -17.2 0 -1.9 GGAAGGATATGATGATGTAA

5730 SEQ ID N0:6150 -3.1 -18 56 -14.9 0 -2.6 CGATGTACATGTTCACCACA

5754 SEQ ID N0:6151 -3.1 -23.4 66.9 -19.6 -0.2 -8.4 CAAACCTCATAGAACATCTC

5842 SEQ ID N0:6152 -3.1 -19.7 59.3 -16.6 0 -2.8 AAATAAAATATCAAGACAGT

6014 SEQ ID N0:6153 -3.1 -13 45.3 -9.9 0 -2.9 GGGGTTTGATGCCATAAACC

6101 SEQ ID N0:6154 -3.1 -24.6 68.7 -19.2 -2.3 -8.6 CTTTGTTTCTTTTTACTTTT

6467 SEQ ID N0:6155 -3.1 -19.3 61.7 -16.2 0 -2.2 TTTACAAAAATAGTGACATA

6893 SEQ ID N0:6156 -3.1 -14.1 47.7 -11 0 -3.5 GGTTTTGAGCAAAAATTTTA

6990 SEQ ID N0:6157 -3.1 -17 53.8 -13 -0.8 -8.1 CATAACAGACTAAAATACTT

7074 SEQ ID N0:6158 -3.1 -14.8 48.9 -11.7 0 -2 GAAGATGGCAAAGCAATACT

7319 SEQ ID N0:6159 -3.1 -19.4 57.9 -15.4 -0.7 -4.3 ATTTAATTGACATAGACTAT

7356 SEQ ID N0:6160 -3.1 -15.9 52.1 -12.8 0 -3.3 TAATGAATGATACAGGATAC

7512 SEQ 2D N0:6161 -3.1 -15.8 51.3 -12.7 0 -2.4 AGCATAAAGTAAAATAAATT

7926 SEQ ID N0:6162 -3.1 -12 43.3 -8.9 0 -4.1 ATTCAAAAGTTTGGAATTGT

7966 SEQ ID N0:6163 -3.1 -16.9 53.8 -13.3 -0.2 -6.5 TTTGACCAATGTATCTCCTA

8080 SEQ ID N0:6164 -3.1 -22.2 65.5 -19.1 0 -3.4 TGTTTGACCAATGTATCTCC

8082 SEQ ID N0:6165 -3.1 -22.8 67.2 -19.7 0 -3.4 TCTTCAATACACTACAGTAA

8378 SEQ ID N0:6166 -3.1 -18.3 57.5 -15.2 0 -3.6 AATAATGACGTAGCTCACCA

8432 SEQ ID N0:6167 -3.1 -21.5 62.2 -18.4 0 -5.3 AGCCAAGGGAGTTAATTGAA

8767 SEQ ID N0:6168 -3.1 -21.1 62.1 -18 0 -3.7 AAATACATATTTAGATCCAA

9042 SEQ ID N0:6169 -3.1 -15.8 51 -12.7 0 -5.7 TTTTTTGACCACAGAGGTTT

96 SEQ ID N0:6170 -3 -22.1 66.2 -17.5 -1.5 -4.7 CTTCCTCTGGCAGCTGTTCA

133 SEQ ID N0:6171 -3 -28.4 82.2 -24.7 0 -8.9 AAACCTTGATGTGGCTTGGC

249 SEQ ID N0:6172 -3 -24.6 69.7 -21.6 0 -3.7 TCTACTATGAATCCTTGACA

2g6 SEQ ID N0:6173 -3 -20.6 62 -17.6 0 -2.3 TTCAGGTCCTGGGGGTACCA

4gg SEQ ID N0:6174 -3 -29.5 83 -23.2 -3.3 -12.6 GAGTTAAAATATACAAGGCA

7gg SEQ ID N0:6175 -3 -16.8 53.1 -13.8 0 -4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TAGTGCACATGATAAGCATG

864 SEQ ID N0:6176 -3 -20.4 61.5 -15.3 -2.1 -9.9 TACTCTACATTCTTTGTCCA

928 SEQ ID N0:6177 -3 -22.6 68.4 -19 -0.3 -2.9 AACCTGGAATGACTGAAATT

1149 SEQ ID N0:6178 -3 -18.5 55.5 -15.5 0 -5.1 TTGTTACATTAACAAATGTC

1380 SEQ ID N0:6179 -3 -16.2 52.6 -10.9 -2.3 -7.5 AAGTGACTGTCATCTCCAAT

1426 SEQ ID N0:6180 -3 -22.2 66.1 -18.5 -0.1 -8.8 CATAGTTGGGGTTTCGACCA

1536 SEQ ID N0:6181 -3 -25.4 72.2 -19.7 -2.7 -6.4 CACTGAAATCTCTTGAAGCA

1872 SEQ ID N0:6182 -3 -20.1 60.3 -17.1 0 -4.1 ACTTTCCAACAGCTCTCCTA

1904 SEQ ID N0:6183 -3 -25.6 73.5 -22.6 0 -4.4 GAGAGACTGATGAGGGGAGC

2129 SEQ ID N0:6184 -3 -24.1 71 -21.1 0 -2.8 TAATAAGGATCCATGGCAAT

2905 SEQ ID N0:6185 -3 -19.9 58.9 -16.2 0 -9 TGAAAACTCTAAGCAGTCTG

3030 SEQ ID N0:6186 -3 -18.9 58.1 -15.9 0 -4.1 CTTGAAAACTCTAAGCAGTC

3032 SEQ ID N0:6187 -3 -19 58.5 -16 0 -4.1 CCTAGAGCCCCCACAGAATT

3100 SEQ ID N0:6188 -3 -28.6 75.5 -25.6 0 -3.8 AAAACAATAAGGCACATGGT

3343 SEQ ID N0:6189 -3 -18.1 54.8 -15.1 0 -5.2 CCTTTTGCATTCTTCCTACT

3492 SEQ ID N0:6190 -3 -25.3 73.1 -22.3 0 -4.8 CAAAGGCCAATGCACCACTA

4128 5EQ ID N0:6191 -3 -24.5 66.8 -20.7 -0.6 -7.7 TTCAAACCGGGATAAGGCTC

4409 SEQ ID N0:6192 -3 -23.1 65.1 -20.1 0 -7.1 ACTAATGTCAAACATGTTAC

4580 SEQ ID N0:6193 -3 -17.1 54.4 -12.9 -1.1 -9 CTTTAAATGTGGCCACTTGA

4704 SEQ ID N0:6194 -3 -22.1 64.2 -16.7 0 -Z3 TAAAGATGACAAAGTATAAA

4806 SEQ ID N0:6195 -3 -12.2 43.7 -9.2 0 -2.8 TATCAAAGACTTGTCTGGTT

5040 SEQ ID N0:6196 -3 -20.2 62.3 -15.7 -1.4 -7 GACAAAACTAGGGTCATGTA

5128 SEQ ID N0:6197 -3 -19.8 60 -15.9 -0.7 -4.7 CGGGTGGTGCACTATTAAGA

5625 SEQ ID N0:6198 -3 -23.8 68.3 -19.5 -1.1 -9.5 TTACCTTCATAGGCTGTAAA

6508 SEQ ID N0:6199 -3 -21.2 63.3 -17 -1.1 -4.4 GTAAAAATATGGCAGATATG

6818 SEQ ID N0:6200 -3 -15.8 50.9 -11.8 -0.9 -4.5 GCCATTACAATTCTTTTTCT

7010 SEQ ID N0:6201 -3 -21.6 64.8 -18.6 0 -2.5 GCATAAAAATAATCCTTTAA

7142 SEQ ID N0:6202 -3 -14.9 48.6 -11.9 0 -3.4 TGCAAAAGACACATACACTG

7476 5EQ ID N0:6203 -3 -18.5 56 -15.5 0 -4.7 TTAATGAATGATACAGGATA

7513 SEQ ID N0:6204 -3 -15.7 51.1 -12.7 0 -2.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTACAAAATGCTGACAAATA

7873 SEQ ID N0:6205 -3 -16.2 51.3 -13.2 0 -4.6 GTGATGTCATCAACCTGAAA

7906 SEQ ID N0:6206 -3 -20.8 61.6 -16.7 -1 -6.9 TGACCAATGTATCTCCTATT

8078 SEQ Ib N0:6207 -3 -22.1 65.1 -19.1 0 -2.4 TTGACCAATGTATCTCCTAT

8079 SEQ ID N0:6208 -3 -22.1 65.1 -19.1 0 -2.9 TCAATAATGACGTAGCTCAC

8434 SEQ ID N0:6209 -3 -19.9 59.9 -16.3 -0.3 -5.4 AGTTCACAAAGCTGCTTTTA

8662 SEQ ID N0:6210 -3 -21.3 64.4 -16.8 -0.3 -10.9 GAATGTAACATTTATTAGCC

8846 SEQ ID N0:6211 -3 -18.5 57,2 -14.6 -0.8 -6.5 CAACTGGAGTTTTCCACTGA

8999 SEQ ID N0:6212 -3 -23.2 67,6 -18.1 -2.1 -7.1 CATTACAACTGGAGTTTTCC

9004 SEQ ID N0:6213 -3 -21.5 64.3 -17.5 -0.9 -5.2 TATGAAATACATATTTAGAT

9046 5EQ ID N0:6214 -3 -13.7 47.2 -9.8 -0.7 -5.2 AACCTTGATGTGGCTTGGCT

248 SEQ ID N0:6215 -2.9 -26.2 73.9 -22.6 -0.4 -3.8 AATTACCTTAGATAGTCACA

408 SEQ ID N0:6216 -2.9 -19.5 60.1 -16.6 0 -2.7 GGCTTTCTCTTCTGCAGCAC

554 SEQ ID N0:6217 -2.9 -27.3 80.7 -23.1 -1.2 -8.3 AGTGCACATGATAAGCATGC

863 SEQ ID N0:6218 -2.9 -22.5 66.2 -17.3 -2.3 -9.9 AGTAAAGGGTCTTTTTGCCC

1459 SEQ ID N0:6219 -2.9 -24.6 71.3 -20.5 -1.1 -4.8 AGAGTAAAGGGTCTTTTTGC

1461 SEQ ID N0:6220 -2.9 -21.2 65.2 -17.8 -0.1 -3.8 CTTCCAAGGTGGCCTGATTC

1761 SEQ ID N0:6221 -2.9 -26.8 75.7 -23 -0.7 -7.2 AAGCTGTCTCTCTCTCCTTT

2020 SEQ ID N0:6222 -2.9 -25.9 77.9 -23 0 -5.1 ACTGAAAATGCTTGTTTTGC

2189 SEQ ID N0:6223 -2.9 -19.7 59.6 -15.9 -0.8 -4.7 GATGACATACTGGCCTGACT

2347 SEQ ID N0:6224 -2.9 -24.5 69.9 -21.6 0 -7.2 ACAATTAAATTCACAAGATG

2722 SEQ ID N0:6225 -2.9 -14.6 48.5 -11.7 0 -3.1 GTCAACACACTACTGAATTG

2830 SEQ ID N0:6226 -2.9 -19.3 58.8 -16.4 0 -3.6 GTGAAAATCCCAGTAAAGAC

2863 SEQ ID N0:6227 -2.9 -19.1 57.1 -16.2 0 -2.1 CCAACTTGAAAACTCTAAGC

3036 SEQ ID N0:6228 -2.9 -18.9 56.6 -16 0 -2.g GCCCCCACAGAATTGCCAAT

3094 SEQ ID N0:6229 -2.9 -29.2 75 -26.3 0 -4.2 CCACAGCAAAAATGAAGACG

3147 SEQ ID N0:6230 -2.9 -18.8 54.8 -15.9 0 -3.3 ATGACCAACATGAAAACAAT

3355 SEQ ID N0:6231 -2.9 -16.4 50.8 -13 -0.1 -5.2 CGATTACGTATTTTTCAACA

3708 SEQ ID N0:6232 -2.g -18.8 56.9 -15.4 -0.1 -6.1 _ TTATGAATGACATATAATCA

3735 SEQ ID N0:6233 -2.9 -15 49.7 -11 -1 -4 kcal) mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CTTCTAGTTCTGACTCACTG

3834 SEQ ID N0:6234 -2.9 -22.5 69.2 -19.1 -0.1 -4 TTCTTCTAGTTCTGACTCAC

3836 SEQ ID N0:6235 -2.9 -22.1 69,4 -18.5 -0.4 -3.4 GAATGAATATATAGGTAAAG

4209 5EQ ID N0:6236 -2.9 -13.6 46.8 -10.7 0 -4.2 TGTTAACATCACTAATGTCA

4590 SEQ ID N0:6237 -2.9 -18.7 58.3 -14.7 -1 -7.9 ACTCAAATTGTTAACATCAC

4598 SEQ ID N0:6238 -2.9 -17.4 54.9 -14 0 -8.3 GATAAAGATGACAAAGTATA

4808 SEQ ID N0:6239 -2.9 -7.4.2 47.9 -11.3 0 -2.8 ATATCAAAGACTTGTCTGGT

5041 SEQ ID N0:6240 -2.9 -20.1 61.9 -15.7 -1.4 -7 ATTAAGAATAGGTGCTAGCA

5612 SEQ ID N0:6241 -2.9 -20 61.1 -15.8 0 -10.6 GAGAAGAGGAGGATCCAGGG

5927 SEQ ID N0:6242 -2.9 -24.2 70.1 -18.7 -2.6 -9 ACACTATCATAGGAAGGAGG

6370 SEQ ID N0:6243 -2.9 -20.6 62.4 -17.7 0 -3.4 CAAAAATAGTGACATAGCAT

6889 SEQ ID N0:6244 -2.9 -16.5 52.2 -13.6 0 -4.1 ATTTGCACTTGCTGAAACAG

7170 SEQ ID N0:6245 -2.9 -20.7 61.5 -17.2 -0.3 -6.5 ATGAATGATACAGGATACAT

7510 SEQ ID N0:6246 -2.9 -17.5 54.8 -14.6 0 -3.2 AGGTGTTTGTAAATAGCTTC

7689 SEQ ID N0:6247 -2.9 -20.2 63.1 -17.3 0 -4.9 GAATTAAAAGTGCAAAAGTA

7710 SEQ ID N0:6248 -2.9 -14.1 47.3 -11.2 0 -5.4 GTAAAATAAATTGTGATGTC

7918 SEQ ID N0:6249 -2.9 -14.6 49 -11.7 0 -3.2 AAATAAGACTAGTCTGCATA

8197 SEQ ID N0:6250 -2.9 -17.7 55.8 -13.3 0 -11 AAAAAATAAGACTAGTCTGC

8200 SEQ ID N0:6251 -2.9 -15.2 49.9 -10.6 0 -12.5 TA~1AAAATAAGACTAGTCT

8202 SEQ ID N0:6252 -2.9 -12.4 44.3 -7.9 0 -11.2 TTAAAAAAATAAGACTAGTC

8203 SEQ ID N0:6253 -2.9 -11.6 42.8 -7.9 0 -9.2 AATAGCTTTAGTGCAGCAGG

8225 SEQ ID N0:6254 -2.9 -23 68.7 -18.5 -1.5 -6.6 GGCTAAAAAGAACAAAGTCC

8258 SEQ ID N0:6255 -2.9 -17.8 54.1 -14.9 0 -3.7 ATAGCCAAGGGAGTTAATTG

8769 SEQ ID N0:6256 -2.9 -20.9 62.3 -18 0 -3.7 ACAACTGGAGTTTTCCACTG

9000 SEQ ID N0:6257 -2.9 -22.8 66.9 -18 -1.9 -5.9 CCAGCATCCAAGATGGTTAG

165 SEQ ID N0:6258 -2.8 -24.4 69.6 -20.1 -1.4 -6.2 AAAGCCCAGCATCCAAGATG

170 SEQ ID N0:6259 -2.8 -24.6 67.5 -20.7 -1 -5.5 GAGCCTTATGAATTACAGCA

194 SEQ ID N0:6260 -2.8 -22.2 65.1 -18.7 -0.4 -4.1 AGCCACTCTACTATGAATCC

292 SEQ ID N0:6261 -2.8 -23.7 68.6 -20.9 0 -3.2 ACAATATCCCTAGAAGAGAT

332 SEQ ID N0:6262 -2.8 -20 59.8 -17.2 0 -3.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTCACAGCACCTTTTTCCGG

394 5EQ ID N0:6263 -2.8 -27.6 76.6 -24.8 0 -6 AAAAAGGCGGAAGCTTTCAG

503 SEQ ID N0:6264 -2.8 -20.1 58.7 -14.9 -2.4 -7.6 GTGCACATGATAAGCATGCT

862 SEQ ID N0:6265 -2.8 -23.4 67.9 -18.8 -1.7 -10.9 CAAAATAGTGCACATGATAA

869 SEQ ID N0:6266 -2.8 -16.5 52 -12.7 0 -9.9 CAGGTCCACAAACTCTGTCA

1079 SEQ ID N0:6267 -2.8 -24.4 70.5 -21.1 -0.1 -3.6 CTGAAATTGTTTTCAGTGCT

1137 SEQ ID N0:6268 -2.8 -20.7 63 -14.5 -3.4 -11.5 CGACTGGATCAGGGCCCCCA

1181 SEQ ID N0:6269 -2.8 -32.3 82.3 -28.1 -0.7 -10.5 CCAATGTAATCCTTCCAGTT

1411 SEQ ID N0:6270 -2.B -24 68.4 -21.2 0 -2.2 TGACTGTCATCTCCAATGTA

1423 SEQ ID N0:6271 -2.8 -22.6 67.4 -19.1 -0.3 -8.1 GTGACTGTCATCTCCAATGT

1424 5EQ ID N0:6272 -2.8 -24.1 71.4 -20.6 -0.3 -$.8 CAAAACATAAAAGTGACTGT

1436 SEQ ID N0:6273 -2.8 -15.2 49.3 -12.4 0 -3.2 TTGCCCATCCAAAACATAAA

1445 SEQ ID N0:6274 -2.8 -20.8 58.7 -18 0 -3 AAGGAAGCTGCTTCTTTTGA

2066 SEQ ID N0:6275 -2.8 -22.1 65,9 -16.7 -1.8 -13.3 TGAAAATGCTTGTTTTGCTA

2187 SEQ ID N0:6276 -2.8 -19.2 58.6 -15.5 -0.8 -4.8 TTTCCTACAGTCAACACACT

2839 SEQ ID N0:6277 -2.8 -22.6 66.7 -19.3 -0.1 -2.9 CTTGAGAACCATTTCTGCTG

2882 SEQ ID N0:6278 -2.8 -22.6 66.1 -18.4 -1.3 -S.8 TCAAAGATATTCCAGCCTTC

2935 SEQ ID N0:6279 -2.8 -22.4 65.7 -18.9 -0.4 -3.8 ATTTTGCCAACTTGAAAACT

3042 SEQ ID N0:6280 -2.8 -18.9 56.6 -16.1 0 -4 TCTATCCACTCTCCACACAG

3289 SEQ ID N0:6281 -2.8 -25.4 73.3 -22.6 0 -1.6 AATAAGGCCAGAAAGAGGTT

3400 SEQ ID N0:6282 -2.8 -20.3 60.1 -17.5 0 -6.9 CTCTCCAACAGCAATTGGCA

3779 SEQ ID N0:6283 -2.8 -25.1 70.5 -20.7 -1.5 -8.1 CTTCCTTCAGATGAGCTGGT

3877 SEQ ID N0:6284 -2.8 -25.8 75.6 -21.8 -1.1 -5 TCCGGTTTAAAGTCTTCTTC

3949 SEQ ID N0:6285 -2.8 -22.5 67.3 -19 -0.5 -7.4 TGAGAAGGATCATGAACACA

4107 SEQ ID N0:6286 -2.8 -19.1 58 -15.6 -0.5 -6.9 AAAGACTTTGTCAGCATATT

4193 SEQ ID N0:6287 -2.8 -19.1 59.2 -15 -1.2 -5.3 ATAAATACATGTACAGATTT

4791 SEQ ID N0:6288 -2.8 -15.5 50.9 -12.2 0 -7,7 AGTTATCTATGATGACACCA

4863 SEQ ID N0:6289 -2.8 -21 63.4 -26.7 -1.4 -5.8 TTTTTAACATAGGCAAAGTT

5497 SEQ ID N0:6290 -2.8 -17.6 55.4 -14.8 0 -4 TCAGGGTCACAGTCGGGTGG

5638 SEQ ID N0:6291 -2.8 -27.9 80.5 -24.3 -0.6 -5.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GCACTTTCTTCAGTAGCAAC

5797 SEQ TD N0:6292 -2.8 -23.2 69.9 -19.5 -0.7 -4.2 TTTGTTGGGTTTTGCTATGA

5945 SEQ ID N0:6293 -2.8 -22.4 67.9 -19.6 0 -3.6 GGATCCGGTCACCACTGACC

5994 SEQ ID N0:6294 -2.8 -29.3 78.4 -23.2 -3.3 0 AATTGATCACAAAGATAATT

6487 SEQ ID N0:6295 -2.8 -14.3 47.9 -10.6 -0.8 -6.3 TGCACAACTATCCCTATAGT

6731 SEQ ID N0:6296 -2.8 -23.4 67.6 -18.8 -1.8 -8.3 CTTTGTCTGATCATTTGAGA

6953 SEQ ID N0:6297 -2.8 -20.7 64.1 -17.9 0 -6.2 AGATAGAAACAAACATAACA

7087 SEQ ID N0:6298 -2.8 -13.8 46.6 -1l 0 -1.2 CTTTGCATAAAAATAATCCT

7146 SEQ ID N0:6299 -2.8 -16.8 52.4 -14 0 -5.1 AGCAGAAAGTGGAACAACTA

7297 SEQ ID N0:6300 -2.8 -19 57.6 -15.2 -0.9 -5.6 TACTGCAGCAGAAAGTGGAA

7303 SEQ ID N0:6301 -2.8 -21.3 62.8 -17.2 -0.7 -10.4 GAATGGTAGAAAATATATTA

8057 SEQ ID N0:6302 -2.8 -13.7 47 -10.9 0 -6.2 ATCTATGAATAAAAGGTTAT

8144 SEQ ID N0:6303 -2.8 -14.7 49.2 -11.4 -0.1 -2.9 CTAGTCTGCATACGTAAATA

8189 5EQ ID N0:6304 -2.8 -19.5 59.3 -16.7 0 -6.6 ATAGCTTTAGTGCAGCAGGA

8224 SEQ ID N0:6305 -2.8 -24.3 72.6 -19.9 -1.5 -6.6 CTTATAGCCAAGGGAGTTAA

8772 SEQ ID N0:6306 -2.8 -21.5 63.8 -18.7 0 -3.7 TAAATCCAAGGGTTCTTGAA

8952 SEQ ID N0:6307 -2.8 -19.7 59.1 -14.8 -2.1 -6.7 ATGCATTACAACTGGAGTTT

9007 SEQ ID N0:6308 -2.8 -20.8 62.7 -18 0 -6.4 AACAGAGCCTTATGAATTAC

198 SEQ ID N0:6309 -2.7 -19.2 58.4 -16.5 0 -3.2 TTCTTGGCTTTCTCTTCTGC

559 SEQ ID N0:6310 -2.7 -25.4 77.3 -22.7 0 -3.7 TCTCTGGAGGAATGTCTCCA

666 SEQ ID N0:6311 -2.7 -25.1 73.7 -19.3 -3.1 -8.2 AGTTAAAATATACAAGGCAG

788 SEQ ID N0:6312 -2.7 -16.2 52 -13.5 0 -4 GTGCTCGGAGAACTCTGAAT

1122 SEQ ID N0:6313 -2.7 -23.1 66.9 -19.7 -0.4 -8.2 TTAAACCTGGAATGACTGAA

1152 SEQ ID N0:6314 -2.7 -18.2 55 -15.5 0 -4.2 TCAGGATCATCACATCAGAA

1212 SEQ ID N0:6315 -2.7 -21 63.6 -17.8 -0.1 -5.3 GTGCTCATTGTTACATTAAC

1387 SEQ ID N0:6316 -2.7 -20.3 62.6 -l7 -0.3 -4.2 GGGTCTTTTTGCCCATCCAA

1453 SEQ ID N0:6317 -2.7 -28.2 77,7 -24 -1.4 -5.4 GAAAATGACCAGGACAAAAA

1679 SEQ ID N0:6318 -2.7 -15.9 49.7 -13.2 0 -4 ATCAAATTCACCAAATAAAA

1708 SEQ ID N0:6319 -2.7 -14 46.6 -11.3 0 -3.1 AATOTATACCAGCATGGCGG

2650 SEQ ID N0:6320 -2.7 -24.4 68.2 -19.7 -2 -7.4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ACTGAATTGCTCAGTCATGG

2819 SEQ ID N0:6321 -2.7 -22.4 66.9 -17.2 -2.5 -10.5 CACAGCACGCGGAACACAAT

3274 SEQ ID N0:6322 -2.7 -23.9 64.7 -20.7 0 -7.9 ACTGCAATCTGCAGATTATT

3475 SEQ ID N0:6323 -2.7 -21.2 63.6 -14.1 -4.4 -13.8 AACAAGCTTCCGGTTTAAAG

3957 SEQ ID N0:6324 -2.7 -20.4 59.7 -17.7 0 -6.6 ATTAGTGAAATATGTTTGAA

4262 SEQ ID N0:6325 -2.7 -15.2 50.4 -12.5 0 -4.1 AAGATGACAAAGTATAAATA

4804 SEQ TD N0:6326 -2.7 -12.9 45.2 -10.2 0 -2.8 AGTGAAGAATGACCCAAAGA

4829 SEQ TD N0:6327 -2.7 -19.7 58 -17 0 -2.1 GATCCGGGACAAAACTAGGG

5135 SEQ ID N0:6328 -2.7 -22.8 63.7 -20.1 0 -6.8 GAACAGAACAATGAACACTA

5159 SEQ ID N0:6329 -2.7 -16.5 51.9 -13.8 0 -3.1 GGGACATCATCAAAGCAAAG

5406 SEQ TD N0:6330 -2.7 -19.8 58.9 -16.5 -0.3 -4.4 GAATTGTGTCAGGGTCACAG

5646 SEQ ID N0:6331 -2.7 -23.1 69.6 -18.8 -1.6 -5.8 TTTTGCTATGAGAAGAGGAG

5936 SEQ ID N0:6332 -2.7 -19.6 60.6 -16.9 0 -3.6 AATATCAAGACAGTGGATCC

6008 SEQ ID N0:6333 -2.7 -20.3 61.2 -17.1 0 -7.5 CTTTGTAAAGGCAAATAAAA

6026 SEQ ID N0:6334 -2.7 -14.5 47.9 -10.2 -1.6 -5 CTCTCACACAAAACACGCTT

6043 SEQ ID N0:6335 -2.7 -21.7 62.1 -19 0 -3.3 CATAAGAGACTTTGGAGGGG

6117 SEQ ID N0:6336 -2.7 -21.6 64.2 -18.9 0 -2.9 AACCTTTGCTTTAAAAGATA

6211 SEQ ID N0:6337 -2.7 -17.4 54.1 -13.9 -0.6 -4.8 TTGATATATTTTTTAACCTT

6225 SEQ ID N0:6338 -2.7 -16.8 54 -14.1 0 -4.3 CTTCATAGGCTGTAAACAAT

6504 SEQ ID N0:6339 -2.7 -19.4 59 -16.7 0 -3.7 AACAGTCAGTTTGGCATGGA

6560 SEQ ID N0:6340 -2.7 -23.4 69.3 -20 -0.5 -5.5 TTCTATCACCAATATGTGAC

7626 SEQ ID N0:6341 -2.7 -20.1 61.2 -16.4 -0.9 -6 CTCATGTTGAATTAAAAGTG

7718 SEQ ID N0:6342 -2.7 -16.1 52 -13.4 0 -4.7 AAATGCTGACAAATAAAGTT

7868 SEQ TD N0:6343 -2.7 -15 48.9 -12.3 0 -3.6 AAAAGAACAAAGTCCATGTT

8253 SEQ ID N0:6344 -2.7 -16.9 52.7 -13.5 -0.4 -4.3 ATTCCCTGCATGCAGTTCTT

8394 SEQ ID N0:6345 -2.7 -27.1 77.5 -21.6 0.3 -13.8 TAATGACGTAGCTCACCATT

8430 SEQ ID N0:6346 -2.7 -22.3 64.5 -19.6 0 -4.8 CAATAAAAAATGAAATTTAT

8463 SEQ ID N0:6347 -2.7 -9.3 38.3 -5.3 -1.2 -5.2 GTAACAAAGAATGTAACATT

8854 SEQ ID N0:6348 -2.7 -14.8 48.9 -11.2 -0.8 -7.2 GAGTTTTCCACTGAGAATAA

8993 SEQ TD N0:6349 -2.7 -19.8 60.3 -16.5 -0.3 -3.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CAGAGCCTTATGAATTACAG

196 SEQ ID N0:6350 -2.6 -20.4 61.2 -17.8 0 -3.2 CTACTATGAATCCTTGACAG

285 SEQ ID N0:6351 -2.6 -20.2 60.9 -17.6 0 -2.4 TCGATAGCAGCAAGAGATTC

529 SEQ ID N0:6352 -2.6 -21.5 64.2 -18.9 0 -5.4 TCACGAAGAAACGTAAAATC

1015 SEQ ID N0:6353 -2.6 -15.4 49.1 -11.9 -0.8 -5.3 TGAATGTTCTCAACGCTGAG

1107 SEQ ID N0:6354 -2.6 -21.2 62.6 -17 -1.6 -6 TGAGCCCAAGAAAATGACCA

1688 SEQ TD N0:6355 -2.6 -22.4 62 -19.8 0 -3.2 TGAAATCTCTTGAAGCAGCT

1869 SEQ ID N0:6356 -2.6 -21 62.8 -18.4 0 -6.1 AACAGCTCTCCTAACCCACC

1897 SEQ ID N0:6357 -2.6 -27.6 74.4 -25 0 -4.4 TCCAGTAGGTGACGTTAGAG

2456 SEQ ID N0:6358 -2.6 -23.6 70.1 -20.4 -0.3 -7 TCATTGATCTTGCAGACACA

3202 SEQ ID N0:6359 -2.6 -22.1 66.1 -19.5 0 -5.2 TACTGCAATCTGCAGATTAT

3476 SEQ ID N0:6360 -2.6 -20.8 62.7 -13.8 -4.4 -Z4 CTTTTGCATTCTTCCTACTG

3491 SEQ ID N0:6361 -2.6 -23.3 69.2 -20.7 0 -5.1 GGGGTAGAACAACATCAACT

3900 SEQ ID N0:6362 -2.6 -21 61.9 -18.4 0 -2.9 ATAGGTAAAGACTTTGTCAG

4199 SEQ ID N0:6363 -2.6 -18.6 58.7 -14.7 -1.2 -5.2 ACCCATTTGAGAAGCATTTC

4231 SEQ ID N0:6364 -2.6 -22.7 66 -19.6 -0.2 -4.1 ACAAGAGCATTCACAACCAC

4438 SEQ TD N0:6365 -2.6 -21.8 63.2 -18.6 -0.3 -4.1 TTAACATCACTAATGTCAAA

4588 SEQ ID N0:6366 -2.6 -16.1 51.8 -11.9 -1.5 -4.9 CTGTTCCTCTGTCATAAAGA

4919 SEQ ID N0:6367 -2.6 -22 66.1 -19.4 0 -2.7 TGTTCCAGCCTATAGTGAAG

5223 SEQ ID N0:6368 -2.6 -23.6 69.3 -21 0 -5.1 AAGTCATCCTCACTCAGGGG

5821 SEQ ID N0:6369 -2.6 -25.8 75.1 -22.3 -0.8 -3.5 AACCTCATAGAACATCTCAA

5840 SEQ ID N0:6370 -2.6 -19.7 59.3 -17.1 0 -2.8 TGCAAAATCAGAGAGTTTAG

5903 SEQ ID N0:6371 -2.6 -17.8 56.1 -14.7 -0.1 -4.7 TCCGGTCACCACTGACCATG

5991 SEQ ID N0:6372 -2.6 -28.2 75.6 -21.2 -4.4 -9.6 AATAAAATATCAAGACAGTG

6013 SEQ ID N0:6373 -2.6 -13.7 46.8 -11.1 0 -4.1 GAAATTACGCTGAATGATAG

6185 SEQ ID N0:6374 -2.6 -16.9 52.7 -14.3 0 -3.3 CTCTGGCAAAACACCTTTGT

6967 SEQ ID N0:6375 -2.6 -23.2 66.1 -19.2 -1.3 -6.6 TGGTTTTGAGCAAAAATTTT

6991 SEQ ID N0:6376 -2.6 -17.3 54.3 -13.8 -0.8 -8.1 AAACTGTAGCCATTACAATT

7018 SEQ ID N0:6377 -2.6 -19.2 57.9 -15.6 -0.9 -3.9 GAAACAAACATAACAGACTA

7082 SEQ ID N0:6378 -2.6 -14.9 48.7 -12.3 0 -2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTGCACTTGCTGAAACAGAA

7168 SEQ ID N0:6379 -2.6 -20.5 60.5 -17.2 -0.5 -6.5 ATTCTATCACCAATATGTGA

7627 SEQ Ib NO:6380 -2.6 -19.9 60.6 -16 -1.2 -6.2 CCAGTGTGTTTCCTTGAAAT

7759 SEQ TD N0:6381 -2.6 -23.1 67.3 -19.7 -0.6 -4.7 TGTACAAAATGCTGACAAAT

7874 SEQ ID N0:6382 -2.6 -16.5 51.8 -13.9 0 -5.9 AAAAAAAAACATCTATGAAT

8154 SEQ ID NO:6383 -2.6 -10.2 39.9 -7.6 0 -3.8 ACGTAAATACTACAAAAGTT

8178 SEQ ID NO:6384 -2.6 -15.1 49.2 -11.8 -0.4 -4.8 CTGCATACGTAAATACTACA

8184 SEQ ID N0:6385 -2.6 -19 57.2 -15.7 -0.4 -6.1 GGTAATTGCACAACTTTGCC

82$9 SEQ ID N0:6386 -2.6 -23.3 66.9 -19.7 -0.9 -5.3 GGTTTGTGCP~AAAAACAAAA

8324 SEQ ID N0:6387 -2.6 -15.6 49.6 -11 -2 -10 GCTTATAGCCAAGGGAGTTA

8773 SEQ ID N0:6388 -2.6 -24 70.3 -20.7 -0.5 -4.2 AGTAACAAAGAATGTAACAT

8855 SEQ ID NO:6389 -2.6 -14.7 48.7 -11.2 -0.8 -6.1 AATTACAGCATAACAAAGCC

184 SEQ ID N0:6390 -2.5 -18.8 56.4 -15.8 -0.2 -4.1 TAGTCACAGCACCTTTTTCC

396 SEQ ID N0:6391 -2.5 -25.3 73.9 -22.8 0 -4.1 ATACGAATTACCTTAGATAG

413 SEQ ID N0:6392 -2.5 -17.6 54.8 -15.1 0 -3.5 CAAGAGATTCTCTAGTAAAA

519 SEQ ID N0:6393 -2.5 -16.4 53.1 -12.6 -1.2 -9.3 ACAGAACACAGTCAGGATCA

1223 SEQ ID N0:6394 -2.5 -2I.8 65.3 -19.3 0 -5.3 CATCTCCAATGTAATCCTTC

1416 SEQ TD N0:6395 -2.5 -22.4 65.9 -19.9 0 -2.4 AGCTGTCTCTCTCTCCTTTG

2019 SEQ ID N0:6396 -2.5 -26.6 80.6 -24.1 0 -4.3 TCCATGGAGAAAAGGAAGCT

2077 SEQ ID N0:6397 -2.5 -21.2 61.5 -17.8 0 -9.5 ATCTTGAGAACCATTTCTGC

2884 SEQ ID N0:6398 -2.5 -22.1 65.8 -18.2 -1.3 -5.5 CGACCTCCATACAGTCCCAC

3315 SEQ ID N0:6399 -2.S -28.7 76.6 -25.7 -0.1 -3.4 GCTGTTCAATGTATATATCT "

4149 SEQ ID N0:6400 -2.5 -20 62.3 -17.5 0 -3.9 ATTTGATGGCACCGAGTTCT

4356 SEQ ID N0:6401 -2.5 -24.7 70.5 -22.2 0 -4 CTGACAGTCACTCAAATTGT

4607 SEQ ID NO:6402 -2.5 -20.8 62.9 -18.3 0 -7.1 GATGATAAAGATGACAAAGT

4811 SEQ ID N0:6403 -2.5 -15.4 50.1 -12.9 0 -2.9 CGGGACAAAACTAGGGTCAT

5131 SEQ ID N0:6404 -2.5 -22.1 63.1 -18.3 -1.2 -S

CGCAGGAAGGGACATCATCA

5414 SEQ ID N0:6405 -2.5 -24.5 69 -21.4 -0.3 -3.9 AGTAGCAACACTGAAGTTCT

5786 SEQ ID N0:6406 -2.5 -21.2 64.5 -18 -0.5 -6.8 GATCAAACTTTTCCCAAACC

5856 SEQ ID N0:6407 -2.5 -21.6 61.5 -19.1 0 -4.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTGCTATGAGAAGAGGAGGA

5934 SEQ ID N0:6408 -2.5 -21.2 63.9 -18.7 0 -3.6 TAAGCTGGACTTTGTTGGGT

5955 SEQ ID N0:6409 -2.5 -23.7 70.2 -20.6 -0.3 -5.1 ACCTTTGCTTTAAAAGATAA

6210 5EQ ID N0:6410 -2.5 -17.4 54.1 -14.1 -0.6 -4.8 TGATATATTTTTTAACCTTT

6224 SEQ ID N0:6411 -2.5 -16.8 54 -14.3 0 -4.3 GTTACTTGATATATTTTTTA

6230 SEQ ID N0:6412 -2.5 -16.4 54.3 -13.9 0 -4.3 TCACAAAGATAATTCTTTGT

6481 SEQ ID N0:6413 -2.5 -17 54.4 -9.8 -4.7 -11 TTCATAGGCTGTAAACAATT

6503 SEQ ID N0:6414 -2.5 -18.6 57.4 -16.1 0 -3.7 AAACAGTCAGTTTGGCATGG

6561 SEQ TD N0:6415 -2.5 -22.1 65.6 -18.4 -1.1 -7.2 CGTAAACCTCATTTACAAAA

6904 SEQ ID N0:6416 -2.5 -17.2 52.6 -12 -2.7 -6.1 TTTGTCTGATCATTTGAGAG

6952 SEQ ID N0:6417 -2.5 -19.8 62.3 -17.3 0 -6.2 ATAAAAATAATCCTTTAAAT

7140 SEQ ID N0:6418 -2.5 -11.7 42.7 -9.2 0 -4.3 TGCATAAAAATAATCCTTTA

7143 SEQ ID N0:6419 -2.5 '15.6 50.2 -13.1 0 -4.7 AAAGGTGTTTGTAAATAGCT

7691 SEQ ID N0:6420 -2.5 -18.3 57.1 -15.8 0 -4.7 ACAAGTTTTAGACATATTTT

7819 SEQ ID N0:6421 -2.5 -16.9 54.8 -13.7 -0.4 -2.9 TGATTATATTTTTACATTAT

7849 SEQ ID N0:6422 -2.5 -14.9 50.3 -12.4 0 -2 AAAGTTGATTATATTTTTAC

7854 SEQ ID N0:6423 -2.5 -14.3 49 -11.8 0 -2.6 GACCAATGTATCTCCTATTG

8077 SEQ ID N0:6424 -2.5 -22.1 65.1 -19.1 -0.1 -3.4 ATTAAAAAAATAAGACTAGT

8204 SEQ ID N0:6425 -2.5 -11.2 41.9 -8.7 0 -6.2 TGCAGCAGGAATTAAAAAAA

8214 SEQ ID N0:6426 -2.5 -15.7 49.8 -13.2 0 -6 AATTCCCTGCATGCAGTTCT

8395 SEQ ID N0:6427 -2.5 -26.3 74.6 -20.7 -0.2 -14.3 CTACTATTATTATCAAACCT

8723 5EQ ID N0:6428 -2.5 -17.8 55.6 -15.3 0 -1.3 CTTGATGTGGCTTGGCTTCA

245 SEQ ID N0:6429 -2.4 -25.9 75.3 -22.8 -0.4 -6 ACCTTGATGTGGCTTGGCTT

247 SEQ ID N0:6430 -2.4 -27 76.7 -23.9 -0.4 -3.8 AATGTGTACTCTACATTCTT

934 SEQ ID N0:6431 -2.4 -19.9 62.2 -16.1 -1.3 -7.4 GTGAATGTGTACTCTACATT

937 SEQ ID N0:6432 -2.4 -20.3 62.8 -16.2 -1.7 -8.2 CTTGCCAAGATTTTTATAAG

979 SEQ ID N0:6433 -2.4 -18.5 57 -16.1 0 -4.1 CATTGTTACATTAACAAATG

1382 SEQ ID N0:6434 -2.4 -15.3 50 -10.1 -2.8 -8.5 AATGTGCTCATTGTTACATT

1390 SEQ ID N0:6435 -2.4 -20.4 62.5 -16.8 -1.1 -3.1 ATGTAATCCTTCCAGTTAAA

1408 5EQ ID N0:6436 -2.4 -20.3 60.9 -179 0 -2.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Tnter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AATGTAATCCTTCCAGTTAA

1409 SEQ ID N0:6437 -2.4 -20.3 60.9 -17.9 0 -2.6 CCATAGTTGGGGTTTCGACC

1537 SEQ ID N0:6438 -2.4 -26.7 74.7 -21.4 -2.9 -7 AATCTCTTGAAGCAGCTGAT

1866 SEQ ID N0:6439 -2.4 -21.7 64.9 -18.6 0 -8.7 CTTTCCAACAGCTCTCCTAA

1903 SEQ ID N0:6440 -2.4 -24.7 70.5 -22.3 0 -4.4 CAACACACTACTGAATTGCT

2828 SEQ ID N0:6441 -2.4 -20.4 60.4 -18 0 -3.6 GGCCAGGATTTTGCCAACTT

3049 SEQ ID N0:6442 -2.4 -26.8 73.9 -22.8 -1.5 -7.4 CATCATTGATCTTGCAGACA

3204 SEQ ID N0:6443 -2.4 -21.9 65.5 -19.5 0 -5.6 GCCACCGTGGGAGCGTACAG

3225 SEQ ID N0:6444 -2.4 -30.1 79.4 -26.6 -1 -9.1 GGAAGAAGTCGTTCATGTGC

3243 SEQ ID N0:6445 -2.4 -22.8 67.4 -19.1 -1.2 -4.9 GACCAACATGAAAACAATAA

3353 SEQ ID N0:6446 -2.4 -15.4 48.9 -13 0 -5.2 CCCTTTTGCATTCTTCCTAC

3493 SEQ ID N0:6447 -2.4 -26.4 74.8 -24 0 -5.1 AATGACATATAATCATTTTC

3730 SEQ ID N0:6448 -2.4 -15.3 50.7 -11.5 -1.3 -7.1 TCCTTCAGATGAGCTGGTTG

3875 SEQ ID N0:6449 -2.4 -24.9 73.4 -21.8 -0.5 -5 CCAGTTGTGCTCAACAATAC

4073 SEQ ID N0:6450 -2.4 -22.4 65.6 -16.7 -3.3 -6.6 ACTTTGTCAGCATATTCTAG

4189 SEQ ID N0:6451 -2.4 -20.9 65.1 -18.5 0 -4.1 TAAAGACTTTGTCAGCATAT

4194 SEQ ID N0:6452 -2.4 -18.7 58.3 -15 -1.2 -5.3 TTGGCTACCAGGCTAACCAA

4321 SEQ ID N0:6453 -2.4 -25.7 70.7 -21.7 -1.5 -7.2 CAAATTGTTAACATCACTAA

4595 SEQ ID N0:6454 -2.4 -15.8 51 -12.9 0 -8 TCAAATTGTTAACATCACTA

4596 SEQ ID N0:6455 -2.4 -16.9 53.9 -14 0 -8.3 AATGACCCAAAGATGATAAA

4822 SEQ ID N0:6456 -2.4 -16.9 51.8 -14.5 0 -2.1 TTGATCCGGGACAAAACTAG

5137 5EQ TD N0:6457 -2.4 -20.5 59.4 -17.6 0 -7.9 CAGAAACATACCTACAATGG

5270 SEQ ID N0:6458 -2.4 -18.7 56 -15.8 -0.1 -3.3 TCCCCTTTGCTCCTTTGATC

5373 SEQ ID N0:6459 -2.4 -28.7 79.8 -26.3 0 -3.9 CAACCAGGAAGGATATGATG

5736 SEQ TD N0:6460 -2.4 -20.1 59.3 -16.9 -0.6 -4 AGAACATCTCAAAGTCATCC

5832 SEQ ID N0:6461 -2.4 -20.5 61.9 -18.1 0 -2.8 GGCAATAAGCTGGACTTTGT

5960 SEQ TD N0:6462 -2.4 -23 67.1 -19.2 -1.3 -5.9 CTCCACTCTCACACAAAACA

6048 SEQ ID N0:6463 -2.4 -22.1 63.6 -19.7 0 0 ATTACGCTGAATGATAGCGG

6182 SEQ ID N0:6464 -2.4 -21.5 61.7 -15.5 -3.6 -8.9 TAACATCTGAAATTACGCTG

6193 SEQ ID N0:6465 -2.4 -17.9 54.7 -15.5 0 -3.3 kcal/

mol koal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGTTACTTGATATATTTTTT

6231 SEQ ID N0:6466 -2.4 -16.7 55 -14.3 0 -4.3 AACAATTGATCACAAAGATA

6490 SEQ ID N0:6467 -2.4 -15.1 49.4 -l2 -0.5 -7.3 ACCTTCATAGGCTGTAAACA

6506 SEQ ID N0:6468 -2.4 -22.3 65.2 -18.7 -1.1 -5.4 GCACAACTATCCCTATAGTC

6730 SEQ ID N0:6469 -2.4 -23.8 69.3 -19.8 -1.5 -7.2 GATGCAAGGACTGTACTAAA

6780 SEQ ID N0:6470 -2.4 -19.6 59.1 -17.2 0 -5.6 CAGACTAAAATACTTTCAAA

7069 SEQ ID N0:6471 -2.4 -14.7 48.7 -12.3 0 -2.5 ACAGACTAAAATACTTTCAA

7070 SEQ ID N0:6472 -2.4 -15.6 50.7 -13.2 0 -2.5 CAGCAGAAAGTGGAACAACT

7298 SEQ ID N0:6473 -2.4 -20 59.3 -16.6 -0.9 -5.6 TGTTTGTAAATAGCTTCAAA

7686 SEQ ID N0:6474 -2.4 -17.1 54.4 -14 -0.5 -4.9 AAAATAATTTTCTTATGTAC

7889 SEQ ID N0:6475 -2.4 -13.4 46.7 -11 0 -4.1 CTACAAAAGTTGAATAAAAA

8169 SEQ ID N0:6476 -2.4 -11.5 42.3 -8.4 -0.4 -3.4 GTAAATACTACAAAAGTTGA

8176 SEQ ID N0:6477 -2.4 -14.7 48.9 -11.6 -0.4 -4.2 TCTGCATACGTAAATACTAC

8185 SEQ ID N0:6478 -2.4 -18.7 57.3 -16.3 0 -6.6 ACTGCCAACAGCAAAAAACA

8518 SEQ ID N0:6479 -2.4 -19.7 56.8 -15.7 -1.5 -4.5 CTATTATTATCAAACCTGAA

8720 SEQ ID N0:6480 -2.4 -16.9 53.2 -13.8 -0.4 -2.9 ACTATTATTATCAAACCTGA

8721 SEQ ID N0:6481 -2.4 -17.8 55.5 -14.9 -0.2 -2.7 AGGGAGTTAATTGAAATTGA

8762 SEQ ID N0:6482 -2.4 -17.3 54.6 -14.9 0 -3.9 GCATTTAAGTAACAAAGAAT

8862 SEQ ID N0:6483 -2.4 -15.3 49.9 -12.9 0 -3.4 TGCATTACAACTGGAGTTTT

9006 SEQ ID N0:6484 -2.4 -20.9 63.1 -18.5 0 -5.2 TGTCTTTAAAAATATGCATT

9020 SEQ ID N0:6485 -2.4 -15.9 51.5 -13.5 0 -6.8 ACAGAGCCTTATGAATTACA

197 SEQ ID N0:6486 -2.3 -20.6 61.5 -18.3 0 -3.2 CCTTGATGTGGCTTGGCTTC

246 SEQ ID N0:6487 -2.3 -27.2 77.9 -24.2 -0.4 -3.8 TTTCCCAGTAAGCCACTCTA

302 SEQ ID N0:6488 -2.3 -26.1 74 -23.8 0 -3.4 AGAAGAGATTCTTTGCTCCT

321 SEQ ID N0:6489 -2.3 -22.7 68 -18.3 -2.1 -8.1 GCTCTGACACCATCTCTGGA

67g SEQ ID N0:6490 -2.3 -26.8 76.8 -22.9 -1.5 -5.5 TTAAAATATACAAGGCAGAG

7g6 SEQ ID N0:6491 -2.3 -15.6 50.6 -13.3 0 -4 GGAGTTAAAATATACAAGGC

790 SEQ ID N0:6492 -2.3 -17.3 54.3 -15 0 -2.8 CAATGTAATCCTTCCAGTTA

1410 SEQ ID N0:6493 -2.3 -21.7 64.2 -19.4 0 -1.9 TGATTCTGCTCCTCATAGGC

1747 SEQ TD N0:6494 -2.3 -25.8 75.6 -22.8 -0.5 -5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Tnter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAGTCATTTTCAGATCCAAC

2230 SEQ ID N0:6495 -2.3 -20.2 61.7 -17.9 0 -4.5 CGTTTCAGTGGTGGTGCCCT

2489 SEQ ID N0:6496 -2.3 -30 83.3 -27.1 -0.3 -5.1 CAAAGATATTCCAGCCTTCT

2934 SEQ ID N0:6497 -2.3 -22.9 66.1 -19.9 -0.4 -3.8 GGGCCAGGATTTTGCCAACT

3050 SEQ ID N0:6498 -2.3 -27.9 76.1 -23.3 -2.3 -7.9 CAGTCATCATTGATCTTGCA

3208 SEQ ID N0:6499 -2.3 -22.7 68.4 -19.7 -0.5 -5.8 CGTACAGTCATCATTGATCT

3212 SEQ ID N0:6500 -2.3 -22 66.1 -19 -0.5 -5,6 TGGGAGCGTACAGTCATCAT

3218 SEQ ID N0:6501 -2.3 -24.8 72.2 -22.5 0 -4.8 CATGACCAACATGAAAACAA

3356 SEQ ID N0:6502 -2.3 -17.1 52 -13 -1.8 -5.2 ATAAGGCCAGAAAGAGGTTC

3399 5EQ ID N0:6503 -2.3 -21.4 63.5 -18.6 -0.1 -7.8 ATTTCATTGTCATCATCAGT

3454 SEQ ID N0:6504 -2.3 -21.1 66 -18.8 0 -2.4 CTAAAAAAGGCTTTTTGGAA

3547 SEQ ID N0:6505 -2.3 -16.5 51.9 -12.7 -1.4 -7.7 ACTCTTCAGTATTTAAGTTT

3810 SEQ ID N0:6506 -2.3 -19.1 61.3 -16.8 0 -2.7 TTCCGGTTTAAAGTCTTCTT

3950 SEQ ID N0:6507 -2.3 -22.2 66.1 -19.3 -0.3 -7.2 ACTGAGAAGGATCATGAACA

4109 SEQ ID N0:6508 -2.3 -19.3 58.6 -16.1 -0.8 -8.2 TGAATATATAGGTAAAGACT

4206 SEQ ID N0:6509 -2.3 -15.4 50.7 -12.6 -0.1 -4.3 CGTAATGATTTGATGGCACC

4363 SEQ ID N0:6510 -2.3 -22.3 63.8 -20 0 -4 AGCAGTGCAAGATAGCCAGC

4684 SEQ ID N0:6511 -2.3 -26.1 74.9 -22.8 -0.7 -9.6 AATCAAAGACCATTCCTTGG

5016 SEQ ID N0:6512 -2.3 -21.2 61.5 -17.7 -1.1 -3.9 GATATCAAAGACTTGTCTGG

5042 SEQ TD N0:6513 -2.3 -19.5 60.1 -15.7 -1.4 -8.9 GGTTGATCCGGGACAAAACT

5139 SEQ ID N0:6514 -2.3 -23.2 64.8 -20.2 -0.3 -8.7 TGTAACTGACAAAAAAGAAA

5715 SEQ ID N0:6515 -2.3 -12.5 44 -9.7 -0.2 -2.9 TTGTAAAGGCAAATAAAATA

6024 SEQ ID N0:6516 -2.3 -13.2 45.4 -10.2 -0.4 -4.8 GTATTTGTTAAAACAGTCAG

6571 SEQ ID N0:6517 -2.3 -17.2 55.3 -14.9 0 -5.6 CAGCAACCTCTTGTCAACGT

6662 SEQ ID N0:6518 -2.3 -24.8 69.8 -21.9 -0.3 -5.5 GTTTTGAGCAAAAATTTTAT

6989 SEQ ID N0:6519 -2.3 -15.8 51.3 -12.6 -0.8 -8.1 GAAGTAACTGAAACTGTAGC

7028 SEQ ID N0:6520 -2.3 -18.2 56.6 -15.9 0 -3.3 AAAATGGAAGTAACTGAAAC

7034 SEQ ID N0:6521 -2.3 -13.7 46.4 -11.4 0 -3 TAGAAAATGGAAGTAACTGA

7037 SEQ ID N0:6522 -2.3 -15.2 49.7 -12.9 0 -3 TAAAATACTTTCAAAATTAA

7064 SEQ ID N0:6523 -2.3 -10.7 41 -8.4 0 -3.2 kcal/

mol kcal/moldeg kcal/molkcal/molkoal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATAGAAACAAACATAACAGA

7085 SEQ ID N0:6524 -2.3 -13.8 46.6 -11.5 0 -1 TAAAATTTGCACTTGCTGAA

7174 SEQ ID N0:6525 -2.3 -18.1 55.4 -15.2 -0.3 -6.5 CTAAAGCAATACTTTAGATA

7275 SEQ ID N0:6526 -2.3 -16 51.9 -10.6 -3.1 -7.9 GAGAGCAGAAGATGGCAAAG

7326 SEQ ID N0:6527 -2.3 -20.5 61 -17.3 -0.8 -4.1 GAATF~1AAP~AACATCTAT

8158 SEQ ID N0:6528 -2.3 -9.9 39.4 -7.6 0 -1.8 TGAATF~AACATCTA

8159 SEQ ID N0:6529 -2.3 -9.9 39.4 -7.6 0 -2.5 AAATACTACAAAAGTTGAAT

8174 SEQ ID N0:6530 -2.3 -13.1 45.4 -10.1 -0.4 -4.2 TATAGCCAAGGGAGTTAATT

8770 SEQ ID N0:6531 -2.3 -20.6 61.8 -18.3 0 -3.7 ACTATATCATTAATACCTTA

8906 SEQ ID N0:6532 -2.3 -17.3 55 -15 0 -4.2 AACTATATCATTAATACCTT

8907 SEQ ID N0:6533 -2.3 -16.9 53.7 -14.6 0 -4.2 GTTCTTGAAAAAAAAGTTAA

8941 SEQ ID N0:6534 -2.3 -12.7 44.8 -10.4 0 -3.5 ATGGGAGAATTATGAAATAC

9056 SEQ ID N0:6535 -2.3 -15.8 51.1 -13.5 0 -3.2 ACAGAGGTTTACAAATTAGT

86 SEQ ID N0:6536 -2.2 -18.3 57.6 -16.1 0 -3.2 TATCCCTAGAAGAGATTCTT

328 SEQ ID N0:6537 -2.2 -21.3 64.1 -16.8 -2.3 -5.3 AAATCTTCTAAGCAAAACCC

1000 SEQ ID N0:6538 -2.2 -18.9 56.2 -16.7 0 -4.1 TAAAATCTTCTAAGCAAAAC

1002 5EQ ID N0:6539 -2.2 -13.9 47.1 -11.7 0 -4.1 AAACCTGGAATGACTGAAAT

1150 SEQ ID N0:6540 -2.2 -17.7 53.6 -15.5 0 -5.1 ACTGGATCAGGGCCCCCACA

1179 SEQ ID N0:6541 -2.2 -31.8 83.1 -28.3 -0.7 -10.4 AATCCTTCCAGTTAAATGTG

1404 SEQ ID N0:6542 -2.2 -20.6 61.4 -18.4 0 -5.6 GTAATCCTTCCAGTTAAATG

1406 SEQ ID N0:6543 -2.2 -20.3 60.9 -18.1 0 -5.2 GAGCCCAAGAAAATGACCAG

1687 SEQ ID N0:6544 -2.2 -22.4 62.3 -20.2 0 -3.2 CCAGGATCAAATTCACCAAA

1713 SEQ ID N0:6545 -2.2 -20.9 59.9 -18.1 -0.3 -5.3 GCATCTGCTGAAATTCGGCC

1797 SEQ ID N0:6546 -2.2 -26 71.6 -21.8 -2 -10.2 ATCCATGGAGAAAAGGAAGC

2078 SEQ ID N0:6547 -2.2 -20.3 59.7 -16.8 -0.4 -10.4 GGTCCACCCACCAAGGAAAC

2431 SEQ ID N0:6548 -2.2 -27.2 72 -24.3 -0.5 -5 TTCCGTTTCAGTGGTGGTGC

2492 SEQ ID N0:6549 -2.2 -27.6 80 -25.4 0 -4.1 CATTGTGTTGGTCAGAATGC

2600 SEQ ID N0:6550 -2.2 -22.5 67.6 -20.3 2.5 -1.2 CAAGTTCTTCCATTGTGTTG

2610 5EQ ID N0:6551 -2.2 -22.4 67.5 -20.2 0 -2.9 TTCTTCAAGTTCTTCCATTG

2615 SEQ ID N0:6552 -2.2 -21.8 66.7 -19.6 0 -2.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GGACATTTCTGTCTAGATTC

2632 SEQ ID N0:6553 -2.2 -21.5 67 -16.6 -2.7 -6.8 ACAATGCAAATAGTGATGGC

2764 SEQ ID N0:6554 -2.2 -19.8 59.2 -17 -0.3 -5.6 ACAGTCAACACACTACTGAA

2833 SEQ ID N0:6555 -2.2 -20.1 60.5 -16.2 -1.7 -5.6 TTTAGTGTGGGCCAGGATTT

3058 SEQ ID N0:6556 -2.2 -25.2 73.8 -22.5 0 -7.6 ATGCCGACCACAGCAAAAAT

3154 5EQ ID N0:6557 -2.2 -22.7 61.9 -19.4 -1 -4.6 CATTGATCTTGCAGACACAT

3201 SEQ ID N0:6558 -2.2 -21.7 64.5 -19.5 0 -5.2 CGTTCATGTGCCACCGTGGG

3234 SEQ ID N0:6559 -2.2 -29.5 78.3 -26.2 -1 -9.1 AAAGAGGTTCAGAACCACAA

3389 SEQ ID N0:6560 -2.2 -19.8 58.9 -15.6 -1.9 -11.2 CTCTTCAGTATTTAAGTTTT

3809 5EQ ID N0:6561 -2.2 -19 61 -16.8 0 -2.7 GGTAGAACAACATCAACTGT

3898 SEQ ID N0:6562 -2.2 -19.8 59.8 -17.1 -0.2 -3.3 ACACAATGAAAGTCTCAAAC

4092 SEQ ID N0:6563 -2.2 -16.7 52.7 -13.9 -0.3 -3.1 ACACAGTGGTAGAACTTGCC

4546 SEQ ID N0:6564 -2.2 -24 69.8 -21.8 0 -6.2 CCAAGTTTCTTCATTGCATT

4951 SEQ ID N0:6565 -2.2 -22.6 67 -20.4 0 -5.1 AATGAACACTAGGTTGATCC

5150 SEQ ID N0:6566 -2.2 -20.4 61.1 -17.4 -0.6 -6 TCAAAGATGTTCCAGCCTAT

5230 SEQ ID N0:6567 -2.2 -23.1 66.9 -20.9 0 -3.2 CCAGCTTCCTTTTTAACATA

5506 SEQ ID N0:6568 -2.2 -22.8 66.3 -20.6 0 -4.5 TTTGCTATGAGAAGAGGAGG

5935 SEQ ID N0:6569 -2.2 -20.7 62.9 -18.5 0 -3.6 TCTGAAATTACGCTGAATGA

6188 SEQ ID N0:6570 -2.2 -18.5 55.9 -16.3 0 -3.3 CAGAGTTGCAGTGACAGAGA

6627 SEQ ID N0:6571 -2.2 -23 69.3 -20.8 0 -5.5 AGCCATTACAATTCTTTTTC

7011 SEQ ID N0:6572 -2.2 -20.7 63 -18.5 0 -3.2 ATGGAAGTAACTGAAACTGT

7031 SEQ ID N0:6573 -2.2 -17.9 55.5 -15.7 , 0 -3 CTATTCTAAAGCAATACTTT

7280 SEQ ID N0:6574 -2.2 -17.1 54.4 -13.7 -1.1 -4.1 TTTAATTGACATAGACTATC

7355 SEQ ID N0:6575 -2.2 -16.3 53.3 -14.1 0 -3.3 TTTTACATTATTTATATTTA

7840 SEQ ID N0:6576 -2.2 -14.2 49.1 -12 0 -2.2 AATGGTAGAAAATATATTAT

8056 SEQ ID N0:6577 -2.2 -13.1 45.8 -10.9 0 -6.2 ACTACAAAAGTTGAATAAAA

8170 SEQ ID N0:6578 -2.2 -12.4 44 -9.5 -0.4 -4.1 TATTATTATCAAACCTGAAA

8719 SEQ ID N0:6579 -2.2 -15.3 49.8 -12.4 -0.4 -2.9 AAGTAACAAAGAATGTAACA

8856 SEQ ID N0:6580 -2.2 -14 47.2 -11.2 -0.3 -5.2 TCAATAACTATATCATTAAT

8912 SEQ ID N0:6581 -2.2 -14.1 48 -11.9 0 -3.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGGGTTCTTGAAAAAAAAGT

8944 SEQ ID N0:6582 -2.2 -16 51.2 -13.8 0 -3.5 GGCAGCTGTTCAGCTTTTTT

125 SEQ ID N0:6583 -2.1 -26 77.4 -22 -1.8 -11 CTATGAATCCTTGACAGGTA

282 SEQ ID N0:6584 -2.1 -21.5 64 -18.4 -0.9 -3.4 TACTATGAATCCTTGACAGG

284 SEQ ID N0:6585 -2.1 -20.5 61.5 -17.6 -0.6 -3.1 CTCAAAGGTATAGATTCCAG

956 SEQ ID N0:6586 -2.1 -20.2 61.4 -17.4 -0.4 -3.6 CACGAAGAAACGTAAAATCT

1014 SEQ ID N0:6587 -2.1 -15.9 49.7 -12.9 -0.8 -5.3 TGACACTGAAATCCAGCCAG

1044 SEQ ID N0:6588 -2.1 -23.4 66 -20.8 -0.2 -5.5 GATCAAATTCACCAAATAAA

1709 SEQ TD N0:6589 -2.1 -15.3 49.2 -13.2 0 -4.7 CTTTAGCACCTTTGGAACTC

1944 SEQ ID N0:6590 -2.1 -23.2 68 -21.1 0 -3.3 TTTTCAGATCCAACATCCTT

2224 SEQ ID N0:6591 -2.1 -22.7 66.5 -20.1 -0.1 -4.5 ACGTTAGAGCTGAAGGTCCA

2445 SEQ ID N0:6592 -2.1 -24.7 70.9 -22.6 0 -5.8 GTGACGTTAGAGCTGAAGGT

2448 SEQ ID N0:6593 -2.1 -23.4 69 -21.3 0 -6.6 GGTGGTGCCCTCTGGGGGAA

2480 SEQ ID N0:6594 -2.1 -31.5 85.5 -27.2 -2.2 -8.7 TTCAAGTTCTTCCATTGTGT

2612 SEQ ID N0:6595 -2.1 -22.8 69.3 -20.7 0 -3.1 TCTTCAAGTTCTTCCATTGT

2614 5EQ ID N0:6596 -2.l -22.9 69.8 -20.8 0 -3.1 TATTTAAGACAATGCAAATA

2772 SEQ ID N0:6597 -2.1 -14.5 48.3 -11.8 -0.3 -5.6 TAGTGTGGGCCAGGATTTTG

3056 SEQ ID N0:6598 -2.1 -25.1 73.2 -22.5 0 -7.6 AGCCCCCACAGAATTGCCAA

3095 SEQ ID N0:6599 -2.1 -29.2 75.3 -27.1 0 -3.2 TAGAGCCCCCACAGAATTGC

3098 5EQ ID N0:6600 -2.1 -27.5 74.2 -25.4 0 -3.2 TCATCATTGATCTTGCAGAC

3205 SEQ ID N0:6601 -2.1 -21.6 65.8 -19.5 0 -5.6 TGTGCCACCGTGGGAGCGTA

3228 SEQ ID N0:6602 -2.1 -30.4 80.5 -27.1 -1.1 -9.1 CCAGAAAGAGGTTCAGAACC

3393 SEQ ID N0:6603 -2.1 -22.2 64.2 -18.4 -1.6 -10.7 ATCTTATTTTTCACATAATC

3517 SEQ ID N0:6604 -2.1 -16.9 55.1 -14.8 0 -2.2 ATGGAAACTTTTTAATACAC

3987 SEQ ID N0:6605 -2.1 -15.7 51.1 -13.6 0 -2.5 TACTTACTTGACAGAATGGA

4002 SEQ ID N0:6606 -2.1 -19 58.5 -16.2 -0.4 -3.2 TTAGTGAAATATGTTTGAAA

4261 SEQ ID N0:6607 -2.1 -14.5 48.8 -12.4 0 -4.1 CCAGGCATTAGTGAAATATG

4268 SEQ ID N0:6608 -2.1 -20.3 60.4 -18.2 0 -4 TGAGTAGCCAAGAGCATTGG

4337 SEQ ID N0:6609 -2.1 -23.7 68.9 -19.5 -2.1 -8.2 CTTCAAACCGGGATAAGGCT

4410 SEQ ID N0:6610 -2.1 -23.6 65.5 -21.5 0 -7.1 kcal/

mot kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTGAATCAACAGCTGCATAC

4740 SEQ ID N0:6611 -2.1 -21.5. 63.8 -18.7 0 -9 AGATGATAAAGATGACAAAG

4812 SEQ ID N0:6612 -2.1 -14.2 47.7 -12.1 0 -2.5 CCAATGAATAGATTCAGAGT

4846 SEQ ID N0:6613 -2.1 -19.2 58.6 -15.9 -1.1 -7.8 GAAGTTATCTATGATGACAC

4865 SEQ ID N0:6614 -2.1 -18.2 57.5 -14.6 -1.4 -5.8 TGATCCGGGACAAAACTAGG

5136 SEQ ID N0:6615 -2.1 -21.6 61.4 -19.5 0 -6.8 CCAGAAACATACCTACAATG

5271 SEQ ID N0:6616 -2.1 -19.5 57.2 -17.4 0 -3 GTTGAACATGTCATCAATTC

5525 SEQ ID N0:6617 -2.l -19.3 60 -15.9 -1 -9.9 TTAAGAATAGGTGCTAGCAA

5611 SEQ ID N0:6618 -2.1 -19.3 59.1 -15.8 0 -10.8 CCCAAACCTCATAGAACATC

5844 SEQ ID N0:6619 -2.1 -22.4 63.2 -20.3 0 -2.5 AGGCAAATAAAATATCAAGA

6018 SEQ ID N0:6620 -2.1 -14.6 48.2 -12.5 0 -4 TAAAGGCAAATAAAATATCA

6021 SEQ TD N0:6621 -2.1 -13 45 -10.9 0 -3.2 GATAACATCTGAAATTACGC

6195 5EQ ID N0:6622 -2.1 -17.6 54.2 -15.5 0 -3.3 TTTGTTTCTTTTTACTTTTG

6466 SEQ ID N0:6623 -2.1 -18.4 59.5 -16.3 0 -2.2 TTGTCTGATCATTTGAGAGA

6951 SEQ 2D N0:6624 -2.1 -20.3 63.3 -18.2 0 -6 CTGGTTTTGAGCAAAAATTT

6992 SEQ ID N0:6625 -2.1 -18.1 55.8 -15.1 -0.8 -8.l TACTTTCAAAATTAAAGCCA

7059 SEQ ID N0:6626 -2.1 -16.9 52.7 -13.9 -0.7 -5.6 ATAAAATTTGCACTTGCTGA

7175 SEQ ID N0:6627 -2.1 -18.8 57.2 -16.1 -0.3 -6.5 TATATATTTGCCCAAACATG

7414 5EQ ID N0:6628 -2.1 -19.3 57.4 -17.2 0 -5.4 ACTCATGTTGAATTAAAAGT

7719 SEQ ID N0:6629 -2.1 -16.3 52.6 -14.2 0 -4.7 TATTGGAATGGTAGAAAATA

8062 SEQ ID N0:6630 -2.1 -15.2 49.9 -13.1 0 -2.2 TTGGCTCAATAATGACGTAG

8439 SEQ ID N0:6631 -2.1 -19.9 59.6 -17.2 -0.3 -5.3 GTTTTATTAATTATATATAT

8543 SEQ ID N0:6632 -2.1 -13.2 46.8 -11.1 0 -5.4 TTATAGCCAAGGGAGTTAAT

8771 SEQ ID N0:6633 -2.1 -20.6 61.8 -18.5 0 -3.7 AACTGGAGTTTTCCACTGAG

ggg8 SEQ ID N0:6634 -2.1 -22.5 66.7 -18.3 -2.1 -6.3 ATGAAATACATATTTAGATC

9045 SEQ ID N0:6635 -2.1 -14.4 48.8 -11.7 -0.3 -5.2 CAAGATGGTTAGGGTATAAC

157 SEQ ID N0:6636 -2 -19.3 59.5 -16.3 -0.9 -3.8 CCAGTAAGCCACTCTACTAT

2g8 SEQ ID N0:6637 -2 -24.3 70 -22.3 0 -4.7 TTACAATATCCCTAGAAGAG

334 SEQ ID N0:6638 -2 -19.2 58.3 -17.2 0 -3.8 TCCTGGGGGTACCAACAGTG

482 SEQ ID N0:6639 -2 -27.3 76 -22.5 -2.8 -10.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GCAGCAAGAGATTCTCTAGT

523 SEQ ID N0:6640 -2 -23.1 70.1 -19.8 -1.2 -9.3 CACTGAATCGGAAAATTGCC

762 SEQ ID N0:6641 -2 -20.5 58.7 -18.5 0 -4.3 AGTGAATGTGTACTCTACAT

938 SEQ ID N0:6642 -2 -20.2 62.7 -17.3 -0.8 -5.5 ATAGATTCCAGTGAATGTGT

947 SEQ ID N0:6643 -2 -2l 64 -17.6 -1.3 -4.1 AGGTCCACAAACTCTGTCAC

1078 SEQ ID N0:6644 -2 -23.9 69.9 -21.4 -0.1 -3.6 TGAGAGCAAACACGCTCAGA

1242 SEQ ID N0:6645 -2 -22.6 64.7 -17.3 -3.3 -8.5 ATGAGAGCAAACACGCTCAG

1243 SEQ ID N0:6646 -2 -22 63.5 -16.7 -3.3 -8.5 GTAAAGGGTCTTTTTGCCCA

1458 SEQ ID N0:6647 -2 -25.3 72.1 -21.8 -1.4 -5.3 TGGGGTTTCGACCAGCCTTC

1530 SEQ ID N0:6648 -2 -28.9 79.8 -24.2 -2.7 -7.8 TTGTTGTTTCCTTCAAGGTG

2002 SEQ ID N0:6649 -2 -23 69.5 -20.2 -0.6 -5.2 CCACAGTGCTGTGCATCTTC

2397 SEQ ID N0:6650 -2 -27 78.3 -22.8 -1.7 -12.4 TCTGGTAAGAGCTTAACCTT

2523 SEQ ID N0:6651 -2 -22.3 66.2 -19.2 -1 -8 GAGCGTACAGTCATCATTGA

3215 SEQ ID N0:6652 -2 -23.1 68.6 -20.5 -0.3 -5.5 CACACAGCACGCGGAACACA

3276 5EQ ID N0:6653 -2 -25.5 68.2 -23 0 -7.9 ATGGTCTCTATCCACTCTCC

3295 SEQ ID N0:6654 -2 -26.6 78.3 -23.7 -0.7 -3.9 TCTTCCTACTGCAATCTGCA

3482 SEQ ID N0:6655 -2 -25.1 72.5 -21 -2.1 -7 TCAATTCCCTTTTGCATTCT

3499 SEQ ID N0:6656 -2 -24 69.6 -22 0 -5.l AGTATTATTGGACATGCAGC

3611 SEQ ID N0:6657 -2 -21.8 65.9 -19.8 0 -5.5 TTTCAAAGTCAGACTCTCCA

3792 SEQ ID N0:6658 -2 -22.3 66.8 -20.3 0 -7.3 TCTAGTTCTGACTCACTGCT

3832 SEQ ID N0:6659 -2 -24.2 73.5 -21.5 -0.4 -4.4 CAACTGTGCTTCCTTCAGAT

3885 SEQ ID N0:6660 -2 -24.2 70.5 -21.3 -0.7 -4 TAGTGAAATATGTTTGAAAT

4260 SEQ ID N0:6661 -2 -14.4 48.5 -12.4 0 -3.5 CATTGGCTACCAGGCTAACC

4323 SEQ ID N0:6662 -2 -26.4 72.9 -23.1 -1.2 -7.2 ATGCCTTCAAACCGGGATAA

4414 SEQ ID N0:6663 -2 -23.5 64.5 -21.5 0 -7.l AAAGTATAAATACATGTACA

4796 SEQ ID N0:6664 -2 -14.2 47.9 -11.5 -0.5 -8.2 GCTGATATCAAAGACTTGTC

5045 SEQ ID N0:6665 -2 -20.1 61.7 -17.4 -0.3 -8.1 AGGGGACACAGAATACTTTT

5303 SEQ ID N0:6666 -2 -21 62.9 -19 0 -1.9 CTCGGAACAAGGTAGGGGAC

5316 SEQ ID N0:6667 -2 -23.9 67.9 -21.9 0 -3 AACATAGGCAAAGTTGGACA

5492 SEQ ID N0:6668 -2 -20.2 60.1 -18.2 0 -4.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATCCATCCCAGCCAGCAGAG

5592 SEQ ID N0:6669 -2 -29.8 80.8 -27.8 0 -4.6 TAAGAATAGGTGCTAGCAAT

5610 SEQ ID N0:6670 -2 -19.2 58.7 -15.8 0 -10.8 AAAAE~1GAAAATCCCAACAG

5705 SEQ ID N0:6671 -2 -14.3 46.6 -12.3 0 -2.2 ACATCTCAAAGTCATCCTCA

5829 SEQ ID N0:6672 -2 -22.6 67.2 -20.6 0 -2.5 AACACGCTTTGTAAAGGCAA

6032 SEQ ID NO:6673 -2 -20.6 59.7 -17 -l.5 -5.9 CTTTGGAGGGGTTTGATGCC

6108 SEQ TD N0:6674 -2 -26.3 75.3 -24.3 0 -3 CACAAAGATAATTCTTTGTT

6480 SEQ ID N0:6675 -2 -16.7 53.5 -9.8 -4.9 -11.3 GAAACTTAGAATAAAATTTG

7185 SEQ ID N0:6676 -2 -11.8 43 -9.8 0 -5.2 TGAAACTTAGAATAAAATTT

7186 SEQ ID N0:6677 -2 -11.8 43 -9.8 0 -4.9 CTTTAAACTATGTTTAATGA

7526 SEQ ID N0:6678 -2 -15.2 50.3 -11.1 -2.1 -8 ATATGAAGAATAGGTTTTGT

7792 SEQ ID N0:6679 -2 -17.1 55 -15.1 0 -l.8 TGCACAACTTTGCCACTTTG

8283 SEQ ID N0:6680 -2 -23.8 67.8 -20.8 -0.9 -4.7 AATGACGTAGCTCACCATTC

8429 SEQ ID N0:6681 -2 -23 66.5 -21 0 -5.3 AACTGCCAACAGCAAAAAAC

8519 SEQ ID NO:6682 -2 -18.3 54.1 -14.7 -1.5 -4.5 TGGAGTTTTCCACTGAGAAT

8995 SEQ ID N0:6683 -2 -22 65.5 -18 -2 -6.2 AGAATTATGAAATACATATT

9051 SEQ ID N0:6684 -2 -13.3 46.1 -10.4 -0.7 -4.6 CAAAGAGACCTTTCCAGAAT

28 SEQ ID N0:6685 -1.9 -20.8 60.8 -17.8 -1 -3.9 GACCACAGAGGTTTACAAAT

90 SEQ ID N0:6686 -1.9 -20.8 61.5 -17.3 -1.5 -7.1 TAACAAAGCCCAGCATCCAA

174 SEQ ID N0:6687 -1.9 -23.9 65.3 -21.1 -0.7 -4.1 TTTCTTGCTCCATAATCTCT

223 SEQ ID N0:6688 -1.9 -23 68.9 -21.1 0 -3.6 CAGCAAGAGATTCTCTAGTA

522 SEQ ID N0:6689 -1.9 -21 64.9 -18.2 -0.7 -8.8 TGTACCAAAATCTTGATAGC

832 SEQ ID N0:6690 -1.9 -19.2 58.3 -17.3 0.2 -4.8 TAAAGAATGTACCAAAATCT

839 SEQ ID N0:6691 -1.9 -15.2 49.3 -13.3 0 -4.2 TCTACATTCTTTGTCCAGTC

925 SEQ ID N0:6692 -1.9 -23.4 71.9 -20.9 -0.3 -3.1 TAAACCTGGAATGACTGAAA

1151 SEQ ID N0:6693 -1.9 -17.4 53.1 -15.5 0 -5.1 GAAAGCTTCTTTACCGACTG

1195 SEQ ID N0:6694 -1.9 -21.9 63.4 -19.1 -0.7 -7 CCACAGCCAGGATCAAATTC

1719 SEQ ID N0:6695 -1.9 -24.1 67.9 -22.2 0 -5.3 ATCTGGTAAGAGCTTAACCT

2524 SEQ ID N0:6696 -1.9 -22.2 65.8 -19.2 -1 -8 TCAAGTTCTTCCATTGTGTT

2611 SEQ ID N0:6697 -1.9 -22.8 69.3 -20.9 0 -3.1 kcal) mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATCATTGATCTTGCAGACAC

3203 SEQ ID N0:6698 -1.9 -21.4 64.8 -19.5 0 -5.4 CCACATGGTCTCTATCCACT

3299 SEQ ID N0:6699 -1.9 -26.5 75.5 -24.1 -0.2 -5.2 TAATAAGGCCAGAAAGAGGT

3401 SEQ ID N0:6700 -1.9 -19.9 59.3 -17.5 0 -7.7 ACATGCAGCTGTCTATCTTA

3600 SEQ ID N0:6701 -1.9 -23.4 70.6 -20.9 0 -8.6 TCAGTATTTAAGTTTTCAAA

3805 SEQ ID N0:6702 -1.9 -16.4 53.8 -14.5 0 -2.7 ACAAGCTTCCGGTTTAAAGT

3956 SEQ ID N0:6703 -1.9 -22.3 64.5 -20.4 0 -6.6 CAGAATGGAAACTTTTTAAT

3991 SEQ ID N0:6704 -1.9 -15.5 50.3 -13.6 0 -2.5 CATGATAGAGGGAATTGCTC

4460 SEQ TD N0:6705 -1.9 -21.1 63.4 -18.7 -0.2 -5.7 CTCAAATTGTTAACATCACT

4597 SEQ ID N0:6706 -1.9 -18.1 56.3 -15.7 0 -8.3 CAAAGATGATAAAGATGACA

4815 SEQ ID N0:6707 -1.9 -14.9 48.8 -13 0 -2.3 TGAAGTTATCTATGATGACA

4866 SEQ ID N0:6708 -1.9 -18 56.9 -14.6 -1.4 -5.8 TATAGTGAAGTAGTAGTGTC

5213 SEQ ID N0:6709 -1.9 -19.1 62.3 -17.2 0 -1.7 TCCAGCTTCCTTTTTAACAT

5507 SEQ ID N0:6710 -1.9 -23.5 68.4 -21.6 0 -4.5 CTCAAAGTTGAACATGTCAT

5531 SEQ ID N0:6711 -1.9 -19 58.4 -16.5 -0.3 -7 ATGTAACTGACAAAAAAGAA

5716 SEQ ID N0:6712 -1.9 -13.2 45.4 -10.5 -0.6 -3.4 GCAAAATCAGAGAGTTTAGA

5902 SEQ ID N0:6713 -1.9 -18.4 57.5 -16 -0.1 -4.4 TTGTTGGGTTTTGCTATGAG

5944 SEQ ID N0:6714 -1.9 -22.3 67.8 -20.4 0 -3.6 TTTGTAAAGGCAAATAAAAT

6025 SEQ ID N0:6715 -1.9 -13.6 46.2 -10.2 -1.4 -4.8 TCTCACACAAAACACGCTTT

6042 SEQ ID N0:6716 -1.9 -20.9 60.7 -19 0 -3.3 GAGGGGTTTGATGCCATAAA

6103 SEQ TD N0:6717 -1.9 -23 66.1 -20 -1 -3.7 TTACTTGATATATTTTTTAA

6229 SEQ ID N0:6718 -1.9 -14.5 49.5 -12.6 0 -4.3 ACGTAAACCTCATTTACAAA

6905 SEQ ID N0:6719 -1.9 -18.1 54.7 -13.5 -2.7 -6.1 ATACTTTCAAAATTAAAGCC

7060 SEQ ID N0:6720 -1.9 -16.2 51.5 -13.4 -0.7 -5.6 GCAGAAAGTGGAACAACTAT

7296 SEQ ID N0:6721 -1.9 -19 57.4 -16.1 -0.9 -4.9 TAACTATTTACATAACATAG

7379 5EQ ID N0:6722 -1.9 -14.2 48.3 -12.3 0 -3.7 TTTACATTATTTATATTTAA

7839 SEQ ID N0:6723 -1.9 -13.4 47.1 -11.5 0 -2.2 AAAATGCTGACAAATAAAGT

7869 SEQ ID N0:6724 -1.9 -14.2 47.2 -12.3 0 -2.9 AAATCCATAAAACCCATTAT

8029 SEQ ID N0:6725 -1.9 -18 53.7 -16.1 0 -2.4 AAATATATTATAACAAAGAA

8047 SEQ ID N0:6726 -1.9 -10.2 40 -8.3 0 -6.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTTTGACCAATGTATCTCCT

8081 SEQ ID N0:6727 -1.9 -23.7 69.3 -21.8 0 -3.4 ACTAGTCTGCATACGTAAAT

8190 SEQ ID N0:6728 -1.9 -20 60.4 -18.1 0 -6.6 AATAAGACTAGTCTGCATAC

8196 SEQ ID N0:6729 -1.9 -18.6 58.3 -l5 0 -11.5 CATGGCTAAAAAGAACAAAG

8261 SEQ ID N0:6730 -1.9 -14.9 48.3 -13 0 -3.7 CCAAGGGTTCTTGAAAAAAA

8947 SEQ ID N0:6731 -1.9 -17.5 53.3 -13.5 -2.1 -5.7 TTTAGATCCAAATTGTCTTT

9033 SEQ ID N0:6732 -1.9 -19 59 -17.1 0 -4.5 AAACAGAGCCTTATGAATTA

199 SEQ ID N0:6733 -1.8 -18.3 56 -16.5 0 -3.2 TGATAAAACAGAGCCTTATG

204 SEQ ID N0:6734 -1.8 -18.2 55.7 -16.4 0 -3.2 TAGAAGAGATTCTTTGCTCC

322 SEQ ID N0:6735 -l.8 -21.5 65.4 -17.4 -2.3 -8.3 AGCAAGAGATTCTCTAGTAA

521 SEQ ID N0:6736 -1.8 -19.6 61.4 -16.5 -1.2 -9.3 ATGTACCAAAATCTTGATAG

833 SEQ ID N0:6737 -1.8 -17.4 54.5 -15 -0.3 -4.8 AATGTACCAAAATCTTGATA

834 SEQ ID N0:6738 -1.8 -16.7 52.6 -14.3 -0.3 -4.8 GAATGTACCAAAATCTTGAT

835 SEQ TD N0:6739 -1.8 -17.6 54.3 -15.2 -0.3 -4.8 GGAATGACTGAAATTGTTTT

1144 SEQ ID N0:6740 -1.8 -17.6 55 -15.8 0 -3.2 AACTTTCCAACAGCTCTCCT

1905 5EQ ID N0:6741 -1.8 -25.2 71.6 -23.4 0 -4.4 TCCTTCAAGGTGCTCTCTCT

1994 SEQ ID N0:6742 -1.8 -26.8 79.3 -24.2 -0.6 -5.3 TCATCAGCAAAGTCATTTTC

2239 SEQ ID N0:6743 -1.8 -20.3 62.8 -18.5 0 -4.1 CTGGCACCATCCTGGATGAC

2361 SEQ ID N0:6744 -1.8 -27.4 75.4 -23.7 -1.2 -11.8 AGCTGAAGGTCCACCCACCA

2438 SEQ ID N0:6745 -1.8 -29.9 79.5 -26.9 -1.1 -5.6 GGTGACGTTAGAGCTGAAGG

2449 SEQ ID N0:6746 -1.8 -23.4 68.3 -21.6 0 -5.4 TAATTAGCATATTTAGTGTG

3069 SEQ ID N0:6747 -1.8 -17.3 55.9 -15.5 0 -5.4 GTACCTACACCACTGGTGGT

3679 SEQ ID N0:6748 -1.8 -27.4 77.3 -23 -2.6 -11.7 ATGAGCTGGTTGCATTTAAT

3867 SEQ ID N0:6749 -1.8 -21.5 64.4 -18.1 -1.5 -5.1 AACCCATTTGAGAAGCATTT

4232 SEQ ID N0:6750 -1.8 -21.6 62.5 -19.8 0 -4.1 AATCCATAAGCAACCCATTT

4243 SEQ ID N0:6751 -1.8 -22.3 62.8 -20.5 0 -4.1 GCTAAAGATCAACCAGAAGA

4502 SEQ ID N0:6752 -1.8 -19.4 58 -17.6 0 -5.4 TCCAGTGAACAGAACAATGA

5165 SEQ ID N0:6753 -1.8 -19.8 58.9 -17.3 -0.5 -3.9 ACAAGCAGATCATGCTGTTG

5559 SEQ ID N0:6754 -1.8 -22.4 66.5 -17.8 -2.8 -10.6 TAGAACATCTCAAAGTCATC

5833 SEQ ID N0:6755 -1.8 -18.2 57.5 -16.4 0 -2.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CTATGAGAAGAGGAGGATCC

5931 SEQ ID N0:6756 -1.8 -21.7 64.7 -18.3 -1.5 -7.7 AAAGGCAAATAAAATATCAA

6020 SEQ ID N0:6757 -1.8 -12.6 44.1 -10.8 0 -4 GTAAAGGCAAATAAAATATC

6022 SEQ ID N0:6758 -1.8 -13.5 46.1 -11.7 0 -4 ACAAAACACGCTTTGTAAAG

6036 SEQ ID N0:6759 -1.8 -17.1 52.6 -14 -1.2 -6.2 CTCTCCACTCTCACACAAAA

6050 SEQ ID N0:6760 -1.8 -22.5 65.1 -20.7 0 0 CCTTTAATTGCCTCTTTGTT

6253 SEQ ID N0:6761 -1.8 -23.7 69 -21.9 0 -3 TTTCTCAAACTTTTCCTTGT

6401 SEQ ID N0:6762 -l.8 -21.2 64.3 -19.4 0 -2.8 AAACAATTGATCACAAAGAT

6491 SEQ ID N0:6763 -1.8 -14.7 48.3 -12 -0.8 -7.2 AGGACTGTACTAAAGGTGTT

6774 SEQ ID N0:6764 -1.8 -20.9 63.8 -19.1 0 -4.8 TGGAAGTAACTGAAACTGTA

7030 SEQ ID N0:6765 -1.8 -17.6 55 -15.8 0 -3.3 TAAATATTAGAGGAGACTTT

7125 SEQ ID N0:6766 -1.8 -16.1 52.6 -13.4 -0.7 -7.1 AATTTGCACTTGCTGAAACA

7171 SEQ ID N0:6767 -1.8 -20 59.4 -18.2 0.4 -5.8 GCAGCAGAAAGTGGAACAAC

7299 SEQ ID N0:6768 -1.8 -20.9 61.3 -18.1 -0.9 -6.2 AAATATGAAGAATAGGTTTT

7794 SEQ ID N0:6769 -1.8 -14.5 48.7 -12.7 0 -2.7 ACAAAAGTTGAATAAAAAAA

8167 SEQ ID N0:6770 -1.8 -9.5 38.7 -7 -0.4 -3.4 AATACTACAAAAGTTGAATA

8173 SEQ ID N0:6771 -l.8 -13.5 46.4 -11 -0.4 -4.2 TAAATACTACAAAAGTTGAA

8175 SEQ ID N0:6772 -1.8 -12.8 44.9 -10.3 -0.4 -4.2 TGCCAACAGCAAAAAACAAA

8516 SEQ ID N0:6773 -1.8 -17.2 51.7 -14 -1.3 -4.1 ATTTAGATCCAAATTGTCTT

9034 SEQ ID N0:6774 -1.8 -18.9 58.7 -17.1 0 -2 CACAGAGGTTTACAAATTAG

87 SEQ ID N0:6775 -1.7 -17.8 55.9 -16.1 0 -3.2 AATCTCTGATAAAACAGAGC

210 SEQ ID N0:6776 -1.7 -17.4 54.7 -12.7 -3 -8.7 TGATGTGGCTTGGCTTCAGT

243 SEQ ID N0:6777 -1.7 -26.1 76.9 -23.7 -0.4 -6 CCCAGTAAGCCACTCTACTA

299 SEQ ID N0:6778 -l.7 -26.3 73.6 -24.6 0 -4.7 TTCCCAGTAAGCCACTCTAC

301 SEQ ID N0:6779 -1.7 -26.2 74.2 -24.5 0 -3.2 ACGAATTACCTTAGATAGTC

411 SEQ ID N0:6780 -1.7 -19.5 59.5 -17.8 0 -3.5 TGGAAGGTTCTTTCCAGCTT

638 SEQ ID N0:6781 -1.7 -25 73.3 -20.6 -2.7 -9.2 TTTCCTAACAGGGTTTAGTG

gOg SEQ ID N0:6782 -1.7 -22.1 66.7 -19.5 -0.7 -4.1 AAAGAATGTACCAAAATCTT

gag SEQ ID N0:6783 -1.7 -15.6 50 -13.3 -0.3 -4.2 CAGTGAATGTGTACTCTACA

gag SEQ ID N0:6784 -1.7 -20.9 64 -18.4 -0.6 -4.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Tntra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TAGATTCCAGTGAATGTGTA

946 SEQ ID N0:6785 -1.7 -20.7 63.4 -17.6 -1.3 -6 AGTGACTGTCATCTCCAATG

1425 SEQ ID N0:6786 -1.7 -22.9 68.3 -20.5 -0.1 -8.8 CACCTTTGGAACTCAACTTT

1938 SEQ ID N0:6787 -l.7 -21.9 63.8 -20.2 0 -4 TGGATGACATACTGGCCTGA

2349 SEQ ID N0:6788 -1.7 -24.6 69.8 -22.9 0 -7.2 ATTGTGTTGGTCAGAATGCT

2599 SEQ ID N0:6789 -1.7 -22.7 68.4 -21 0 0 GTACAGTCATCATTGATCTT

3211 SEQ ID N0:6790 -1.7 -21.3 66.3 -18.9 -0.5 -5.8 TTTCATTGTCATCATCAGTA

3453 SEQ ID N0:6791 -1.7 -20.8 65.4 -19.1 0 -2.4 AGTACCTACACCACTGGTGG

3680 SEQ ID N0:6792 -l.7 -26.2 74.2 -21.9 -2.6 -11 CAGTATTTAAGTTTTCAAAG

3804 SEQ ID N0:6793 -1.7 -16 52.7 -14.3 0 -2.7 TTCAGTATTTAAGTTTTCAA

3806 SEQ TD N0:6794 -1.7 -17.2 56.1 -15.5 0 -2.7 TCTTCAGTATTTAAGTTTTC

3808 SEQ ID N0:6795 -1.7 -18.5 60.4 -16.8 0 -2.7 CTACTGAGAAGGATCATGAA

4111 SEQ ID N0:6796 -1.7 -19 58.2 -16.4 -0.8 -8.2 CATAAGCAACCCATTTGAGA

4239 SEQ ID N0:6797 -1.7 -21.8 62.5 -19.4 -0.4 -3.3 GTTATCTATGATGACACCAA

4862 SEQ ID N0:6798 -1.7 -20.3 61.1 -17.8 -0.6 -5.1 CAAAATCAAAGACCATTCCT

5019 SEQ ID N0:6799 -1.7 -19.2 56.5 -17.5 0 -2.8 AGAACAATGAACACTAGGTT

5155 SEQ ID N0:6800 -1.7 -18.2 56.2 -15.8 -0.4 -6 TGGAACAAGCAGATCATGCT

5563 SEQ ID N0:6801 -1.7 -22.2 64.7 -17.1 -3.4 -11.7 TTGGAACAAGCAGATCATGC

5564 SEQ ID N0:6802 -1.7 -21.4 63.2 -16.3 -3.4 -11.9 GGTGCTAGCAATCCATCCCA

5602 SEQ ID N0:6803 -1.7 -28.8 78.6 -25.6 0 -10.9 TCGGGTGGTGCACTATTAAG

5626 SEQ ID N0:6804 -1.7 -23.6 68.5 -20.6 -1.1 -9.5 TGTGTCAGGGTCACAGTCGG

5642 SEQ ID N0:6805 -1.7 -26.7 78.5 -23.5 -1.4 -5.7 TTAAAAGATAACATCTGAAA

6201 SEQ ID N0:6806 -1.7 -12.7 44.7 -10.4 -0.3 -3.7 TAACCTTTGCTTTAAAAGAT

6212 SEQ ID N0:6807 -1.7 -17.4 54.1 -14.9 -0.6 -4.8 AGCAACCTCTTGTCAACGTT

6661 SEQ ID N0:6808 -1.7 -24.2 69.1 -21.9 -0.3 -6.9 TTGCACAACTATCCCTATAG

6732 SEQ ID N0:6809 -1.7 -22.3 64.8 -19.7 -0.8 -6.6 TTTGCACAACTATCCCTATA

6733 SEQ ID N0:6810 -1.7 -22.4 64.9 -20.7 0 -5 ATTTTGTAAAAATATGGCAG

6823 SEQ ID N0:6811 -1.7 -15.8 51.1 -12.4 -1.7 -7.4 GATTATATTTTTACATTATT

7848 SEQ ID N0:6812 -1.7 -15 50.7 -13.3 0 -2.2 AATTGTGATTAAAAATCAAA

7g52 SEQ ID N0:6813 -1.7 -11.9 43.1 -8.6 -1.5 -7.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CATCTATGAATAAAAGGTTA

8145 SEQ ID N0:6814 -1.7 -15.4 50.4 -13.7 0 -2.5 AAACTTTTGGTTTGTGCAAA

8332 SEQ ID N0:6815 -1.7 -18.9 57.7 -16.1 -1 -8.8 GAATTAACATTAAACTTTTG

8343 5EQ ID N0:6816 -1.7 -13.7 46.9 -12 0 -3.5 GCCAACAGCAAAAAACAAAA

8515 SEQ ID N0:6817 -1.7 -16.5 50.2 -14 -0.6 -4.1 AAGTTCACAAAGCTGCTTTT

8663 SEQ ID N0:6818 -1.7 -20.9 62.8 -17.7 -0.3 -10.9 GGGAGTTAATTGAAATTGAA

8761 SEQ ID N0:6819 -1.7 -16.6 52.7 -14.9 0 -3.9 AAGAATGTACCAAAATCTTG

837 SEQ ID N0:6820 -1.6 -16.3 51.6 -14.7 0 -4.8 TTGCTCAAGGTCATAAATAC

898 SEQ ID N0:6821 -1.6 -18.9 58.4 -17.3 0 -3.6 ACACGCTCAGACAGAACACA

1233 5EQ ID N0:6822 -1.6 -22.9 65.3 -21.3 0 -3.4 GGGGTTTCGACCAGCCTTCA

1529 SEQ ID N0:6823 -l.6 -29.6 81 -25.3 -2.7 -7.7 TGAGTCATGAGTCGAAATAG

1597 SEQ ID N0:6824 -1.6 -18.8 58.1 -15.5 0 -11.5 CAGCTCTCCTAACCCACCTA

1895 SEQ ID N0:6825 -1.6 -28.7 77.5 -27.1 0 -4.4 AGAGACTGATGAGGGGAGCA

2128 SEQ ID N0:6826 -1.6 -24.2 70.8 -22.6 0 -4.1 GTGGTGGTGCCCTCTGGGGG

2482 SEQ ID N0:6827 -1.6 -32.8 90.6 -29 -2.2 -8.2 CATCTCCATTGAAATCTGGT

2537 SEQ ID N0:6828 -1.6 -22.1 65 -20.5 0.3 -3.1 GGCGGACATTTCTGTCTAGA

2635 SEQ ID N0:6829 -1.6 -24.8 72.3 -20.5 -2.7 -8.6 CAATTAAATTCACAAGATGT

2721 SEQ ID N0:6830 -1.6 -15.6 50.6 -14 0 -3.1 CTACAGTCAACACACTACTG

2835 SEQ ID N0:6831 -1.6 -20.8 62.6 -17.8 -1.3 -5.1 GGCAATGATCTTGAGAACCA

2891 SEQ ID N0:6832 -1.6 -22.4 64.6 -20.1 -0.4 -6.2 TTTTGCCAACTTGAAAACTC

3041 SEQ ID N0:6833 -1.6 -19.3 57.8 -17.7 0 -3.1 TGGTCTCTATCCACTCTCCA

3294 SEQ ID N0:6834 -1.6 -27.3 79.4 -25.1 -0.3 -3.7 ACCAACATGAAAACAATAAG

3352 SEQ ID N0:6835 -l.6 -14.8 47.9 -13.2 0 -4.3 TAGCAGGTTTTTCGAAGATT

4051 SEQ ID N0:6836 -1.6 -20.8 62.9 -18.5 -0.4 -6.8 AGTTGTGCTCAACAATACTG

4071 SEQ ID N0:6837 -1.6 -20.6 62.4 -15.7 -3.3 -6.6 GGATCATGAACACAATGAAA

4101 SEQ ID N0:6838 -1.6 -17.1 52.9 -14.6 -0.8 -7.4 CCATAAGCAACCCATTTGAG

4240 SEQ ID N0:6839 -1.6 -23.2 64.7 -20.9 -0.4 -4.1 GCCTTCAAACCGGGATAAGG

4412 SEQ ID N0:6840 -1.6 -24.7 67.1 -22.2 -0.8 -8.6 ~

ATAGAGGGAATTGCTCCAAC

4456 SEQ ID N0:6841 -1.6 -22 64.4 -18.6 -1.8 -6.1 AAATTGTTAACATCACTAAT

4594 SEQ ID N0:6842 -1.6 -15.1 49.7 -13 0 -8.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformation,Duplexstructureoligo oligo GAATCAACAGCTGCATACAT

4738 SEQ ID N0:6843 -1.6 -21 62 -18.7 0 -8.9 TGAATCAACAGCTGCATACA

4739 SEQ ID N0:6844 -l.6 -21 61.9 -18.7 0 -8.9 AAAGTCAAAGATGTTCCAGC

5234 SEQ ID N0:6845 -l.6 -20.3 6l -18.7 0 -2.9 CTTTGCTCCTTTGATCAGAC

5369 SEQ ID N0:6846 -1.6 -23.8 70.5 -22.2 0 -6.5 TGAACATGTCATCAATTCCA

5523 SEQ ID N0:6847 -1.6 -20.7 61.6 -18.4 -0.5 -7.2 CTTTGTTGGGTTTTGCTATG

5946 SEQ ID N0:6848 -1.6 -22.7 68.6 -21.1 0 -3.6 TACGCTGAATGATAGCGGCA

6180 SEQ ID N0:6849 -1.6 -23.9 66.3 -18.7 -3.6 -10.6 TTTAAAAGATAACATCTGAA

6202 SEQ ID N0:6850 -1.6 -13.5 46.4 -11 -0.8 -6.5 AGCAGTGTCAGCTGGTAATA

6686 SEQ ID N0:6851 -l.6 -23.8 71.7 -20.6 -1.6 -8.6 AGATGGCAAAGCAATACTGC

7317 SEQ ID N0:6852 -1.6 -21.3 62.3 -18.2 -1.4 -6 TTAATTGACATAGACTATCA

7354 SEQ ID N0:6853 -1.6 -16.9 54.3 -15.3 0 -3.3 TATATTTGCCCAAACATGCA

7412 SEQ ID N0:6854 -1.6 -22.1 62.9 -19.8 -0.5 -5.4 TCTATCACCAATATGTGACA

7625 SEQ ID N0:6855 -1.6 -20.7 62 -17.6 -1.4 -6.5 TGTAAATAGCTTCAAATTAT

7682 SEQ ID N0:6856 -1.6 -15.5 50.8 -13.9 0 -4.6 TTTTTACATTATTTATATTT

7841 SEQ ID N0:6857 -1.6 -14.6 50 -13 0 -2.2 ATTGTGATTAAAAATCAAAA

7951 SEQ ID N0:6858 -1.6 -11.9 43.1 -8.3 -2 -7 ATGGTAGAAAATATATTATA

8055 SEQ ID N0:6859 -1.6 -13.5 46.8 -11.9 0 -6.2 TACAAAAGTTGAATAAAAAA

8168 SEQ ID N0:6860 -l.6 -9.9 39.4 -7.6 -0.4 -3.4 CAGGTTTTATTAATTATATA

8546 SEQ ID N0:6861 -1.6 -15.4 51.4 -13.8 0 -4.2 TTGTTGTAAAATTCATGTAA

8597 SEQ ID N0:6862 -1.6 -15.6 51.3 -13.3 -0.4 -6.6 GGGTTCTTGAAAAAAAAGTT

8943 SEQ ID N0:6863 -1.6 -16.1 51.4 -14.5 0 -3.5 TTTTTCGATAGCAGCAAGAG

533 SEQ ID N0:6864 -1.5 -20.8 62.5 -19.3 0 -5.4 CAGAGGTGGCACTGAATCGG

771 SEQ ID N0:6865 -1.5 -24.8 70 -23.3 0 -4 GGTTTAGTGGAGTTAAAATA

798 SEQ ID N0:6866 -1.5 -17.9 56.8 -16.4 0 -2.8 TCTGAATGTTCTCAACGCTG

1109 SEQ ID N0:6867 -1.5 -21.9 64.4 -19.5 -0.8 -5.3 GAAATCTCTTGAAGCAGCTG

1868 SEQ ID N0:6868 -1.5 -21 62.8 -19 0 -8.2 GTTCTTCCATTGTGTTGGTC

2607 5EQ ID N0:6869 -1.5 -25.2 76.9 -23 -0.4 -3.7 ATCCATAACAATTAAATTCA

2729 SEQ ID N0:6870 -1.5 -15.9 50.9 -14.4 0 -3.1 CATGGCAATGATCTTGAGAA

2894 SEQ ID N0:6871 -1.5 -20.2 60.3 -18.7 0 -5.5 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GCGTACAGTCATCATTGATC

3213 SEQ ID N0:6872 -1.5 -22.9 68.5 -20.7 -0.5 -5.7 CTCTATCCACTCTCCACACA

3290 SEQ ID N0:6873 -1.5 -26.3 75 -24.8 0 -0.9 GCTTCCTTCAGATGAGCTGG

3878 SEQ ID N0:6874 -1.5 -26.4 76.6 -23.7 -1.1 -5.2 GAATATATAGGTAAAGACTT

4205 SEQ ID N0:6875 -1.5 -15.5 51 -13.5 -0.1 -4.3 AGATGAGACAGACCAACAGC

4485 SEQ ID N0:6876 -1.5 -22.2 64.8 -20.7 0 -2.8 CTAAAGATCAACCAGAAGAT

4501 SEQ ID N0:6877 -1.5 -17.6 54.2 -16.1 0 -4.6 CGTGAATCAACAGCTGCATA

4741 SEQ ID N0:6878 -1.5 -22.1 63.6 -19.9 0 -9 GAACAATGAACACTAGGTTG

5154 SEQ ID N0:6879 -1.5 -18.2 56 -15.9 -0.6 -6 TTGTTACACTATCATAGGAA

6375 SEQ ID N0:6880 -l.5 -18.7 58.6 -17.2 0 -3.4 ATTCTTTGTTTCTTTTTACT

6470 SEQ ID N0:6881 -1.5 -19.5 62.5 -18 0 -2.2 TAAACAATTGATCACAAAGA

6492 SEQ ID N0:6882 -1.5 -14.4 47.8 -l2 -0.8 -7.2 CTGGTAATAAAAACAGCAAC

6675 5EQ ID N0:6883 -1.5 -16.1 50.9 -14.6 0 -4.1 CAAGGACTGTACTAAAGGTG

6776 SEQ ID N0:6884 -1.5 -19.6 59.5 -18.1 0 -4.8 TGCAGCAGAAAGTGGAACAA

7300 SEQ ID N0:6885 -1.5 -20.7 60.7 -18.2 -0.9 -7.5 CTATCACCAATATGTGACAT

7624 SEQ ID N0:6886 -1.5 -20.3 60.7 -17.3 -1.4 -6.5 ATGTACAAAATGCTGACAAA

7875 SEQ ID N0:6887 -1.5 -16.5 51.8 -15 0 -6.8 AAGCATAAAGTAAAATAAAT

7927 SEQ ID N0:6888 -1.5 -11.2 41.7 -9.7 0 -4.1 ACAAAAACAAAAAACCCCAG

8501 SEQ ID N0:6889 -l.5 -16.4 49.6 -14.9 0 0 AGATAGCATCAATTATATTG

8812 SEQ ID N0:6890 -1.5 -16.8 54.2 -14.4 -0.7 -5.7 GTAACATTTATTAGCCACCA

8842 SEQ ID N0:6891 -l.5 -22.2 64.7 -20.7 0 -3.2 TTCCACTGAGAATAAGAGTT

8988 SEQ ID N0:6892 -1.5 -19.7 60.2 -18.2 0 -2.8 TATGCATTACAACTGGAGTT

9008 SEQ ID N0:6893 -1.5 -20.4 61.8 -18.9 0 -6.8 TTAGATCCAAATTGTCTTTA

9032 SEQ ID N0:6894 -1.5 -18.6 58.1 -17.1 0 -4.6 AAAACAGAGCCTTATGAATT

200 SEQ ID N0:6895 -l.4 -17.9 54.8 -16.5 0 -3.2 CTAGAAGAGATTCTTTGCTC

323 SEQ ID N0:6896 -1.4 -20.4 63.5 -16.7 -2.3 -8.3 CATACGAATTACCTTAGATA

414 SEQ ID N0:6897 -1.4 -18.3 55.8 -16.9 0 -3.5 GGTCCTGGGGGTACCAACAG

484 SEQ ID N0:6898 -1.4 -28.5 78.7 -24.6 -2.5 -10.2 CGATAGCAGCAAGAGATTCT

528 SEQ ID N0:6899 -1.4 -22 64.7 -20.6 0 -4.6 GATTCCAGTGAATGTGTACT

944 SEQ ID N0:6900 -1.4 -22.1 66.3 -19.3 -1.3 -7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TGCTCGGAGAACTCTGAATG

1121 SEQ ID N0:6901 -1.4 -21.9 63.7 -19.8 -0.4 -8.2 CAGTGCTCGGAGAACTCTGA

1124 SEQ TD N0:6902 -l.4 -24.5 70.6 -22.2 -0.7 -7.9 CACGCTCAGACAGAACACAG

1232 SEQ ID N0:6903 -1.4 -22.7 65 -21.3 0 -3.4 CTGTCATCTCCAATGTAATC

1420 SEQ ID N0:6904 -1.4 -21.5 64.8 -19.5 -0.3 -3 GAGTAAAGGGTCTTTTTGCC

1460 SEQ ID N0:6905 -1.4 -23.2 68.9 -21.1 -0.4 -4.l CAAAGCTTGTGTAGCCATAG

1551 SEQ ID N0:6906 -1.4 -22.4 65.8 -19.4 -l.5 -7.9 CCCAAGAAAATGACCAGGAC

1684 SEQ ID N0:6907 -1.4 -22 61.3 -20.6 0 -4 AGCCCAAGAAAATGACCAGG

1686 SEQ ID N0:6908 -1.4 -23 63.4 -21.6 0 -3.6 TCTCTTGAAGCAGCTGATGC

1864 SEQ ID N0:6909 -1.4 -24.2 71.5 -21.2 -1.6 -8.7 ATTCTTTAGCACCTTTGGAA

1947 5EQ ID N0:6910 -1.4 -22.2 65.8 -20.8 0 -4.1 CATTCTTTAGCACCTTTGGA

1948 SEQ ID N0:6911 -1.4 -23.6 69.2 -22.2 0 -4.1 AGGTCCACCCACCAAGGAAA

2432 SEQ ID N0:6912 -1.4 -27 71.7 -24.4 -1.1 -5.8 TCTTCCATTGTGTTGGTCAG

2605 SEQ ID N0:6913 -1.4 -24.6 74.2 -22.5 -0.4 -3.7 CTTCAAGTTCTTCCATTGTG

2613 SEQ ID N0:6914 -1.4 -22.5 67.9 -21.1 0 -3.1 CAGCATGGCGGACATTTCTG

2641 SEQ ID N0:6915 -l.4 -25.2 70.9 -22.9 -0.7 -5.4 GATTTTGCCAACTTGAAAAC

3043 5EQ ID N0:6916 -1.4 -18.6 56 -17.2 0 -4 CATGTGCCACCGTGGGAGCG

3230 SEQ ID N0:6917 -1.4 -30.2 78.7 -27.7 -1 -9.1 CAGAAAGAGGTTCAGAACCA

3392 SEQ ID N0:6918 -1.4 -20.9 61.7 -16.8 -2.7 -12 TTGCATTCTTCCTACTGCAA

3488 SEQ ID N0:6919 -1.4 -24 69.7 -20.5 -2.1 -6.8 TACTGAGAAGGATCATGAAC

4110 SEQ ID N0:6920 -l.4 -18.3 56.9 -16 -0.8 -8.2 CCTTCAAACCGGGATAAGGC

4411 SEQ ID N0:6921 -1.4 -24.7 67.1 -22.7 -0.3 -7.6 ATCTCAGCCAGAAACATACC

5278 SEQ ID N0:6922 -1.4 -22.9 65.8 -21.5 0 -3.6 CAATCCATCCCAGCCAGCAG

5594 SEQ ID N0:6923 -1.4 -29.2 77.7 -27.8 0 -4.6 GTCACAGTCGGGTGGTGCAC

5633 SEQ ID N0:6924 -l.4 -28.3 81.6 -25.8 -0.6 -9.9 GCTGTAAACAATTGATCACA

6496 SEQ ID N0:6925 -1.4 -19.1 57.9 -17.7 0 -7.2 GTCTTGTATAGGCACTGACT

6595 SEQ ID N0:6926 -1.4 -23.7 71.5 -21.3 -0.9 -4.3 TAGAAACAAACATAACAGAC

7084 SEQ ID N0:6927 -1.4 -14 47 -12.6 0 -1 ATACTGCAGCAGAAAGTGGA

7304 SEQ ID N0:6928 -1.4 -22 64.9 -19.3 -0.7 -10.4 TGGCAAAGCAATACTGCAGC

7314 SEQ ID N0:6929 -l.4 -23.2 66.2 -19.3 -2.5 -9.6 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGATACCATATGATACTCAT

7733 SEQ ID N0:6930 -1.4 -19.4 59.4 -17.2 -0.6 -6 CAAAAGTTGAAT~

8166 SEQ ID N0:6931 -1.4 -8.6 37.2 -7.2 0 -2.6 TAGTCTGCATACGTAAATAC

8188 SEQ ID N0:6932 -1.4 -18.8 57.9 -17.4 0 -6.6 AAAAAAATAAGACTAGTCTG

8201 SEQ ID N0:6933 -1.4 -12.7 44.8 -9.6 0 -11.5 CAAAAAACAAAAATTTATAT

8316 SEQ ID N0:6934 -1.4 -8.9 37.6 -7.5 0 -5.2 ATGTGAACTTGATCCTTTGC

8691 SEQ ID N0:6935 -1.4 -22.2 65.8 -20.8 0 -4.3 TAACATTTATTAGCCACCAA

8841 SEQ ID N0:6936 -1.4 -20.3 59.7 -18.9 0 -3.2 ACAAATTAGTTACAATTTTT

76 SEQ 2D N0:6937 -1.3 -14.9 49.7 -12.9 -0.5 -4 AGAGCCTTATGAATTACAGC

195 SEQ ID N0:6938 -1.3 -21.5 64.1 -20.2 0 -3.2 CTCCATAATCTCTGATAAAA

216 SEQ ID N0:6939 -l.3 -17.8 55.1 -16.5 0 -3.3 TCCCAGTAAGCCACTCTACT

300 SEQ ID N0:6940 -1.3 -27 75.8 -25.7 0 -4.5 TGGCTTTCTCTTCTGCAGCA

555 SEQ ID N0:6941 -1.3 -27.1 79.8 -24.5 -1.2 -8.3 GAGACAGGAAAGCCCAGCTA

1578 SEQ ID N0:6942 -1.3 -25.4 70.8 -23.1 -0.9 -6 AAAATGAGCCCAAGAAAATG

1692 SEQ ID N0:6943 -1.3 -16.8 51.2 -15.5 0 -3.2 AAATCTCTTGAAGCAGCTGA

1867 SEQ ID N0:6944 -1.3 -21 62.8 -19 0 -8.7 CAACAGCTCTCCTAACCCAC

1898 SEQ ID N0:6945 -1.3 -26.3 72.1 -25 0 -4.4 GTTGTTTCCTTCAAGGTGCT

2000 SEQ ID N0:6946 -1.3 -25.6 75.9 -23.5 -0.6 -5.3 ATTTTCAGATCCAACATCCT

2225 SEQ ID N0:6947 -1.3 -22.6 66.1 -20.8 -0.1 -4.5 CACACCATTGCAATCCACAG

2411 SEQ ID N0:6948 -1.3 -24.4 67.8 -22.5 0 -8.6 GGTTTCCTACAGTCAACACA

2841 SEQ ID N0:6949 -1.3 -23.9 70.1 -22.1 -0.2 -3.6 AGATATTCCAGCCTTCTTGG

2931 SEQ ID N0:6950 -1.3 -24.9 72.4 -23.1 -0.2 -6.7 GCCGACCACAGCAAAAATGA

3152 SEQ ID N0:6951 -1.3 -23.3 63 -22 0 -4.1 AGTCATCATTGATCTTGCAG

3207 SEQ ID N0:6952 -1.3 -22 67.5 -20 -0.5 -6.4 TTCTTCCTACTGCAATCTGC

3483 SEQ ID N0:6953 -1.3 -24.5 71.7 -22.1 -1 -4.9 CACCACTGGTGGTTCCATTC

3672 SEQ ID N0:6954 -l.3 -27.2 77.1 -23.8 -1.7 -12 TCAAAGTCAGACTCTCCAAC

3790 SEQ ID N0:6955 -1.3 -21.6 64.5 -20.3 0 -7.3 GTTGTGCTCAACAATACTGT

4070 SEQ ID N0:6956 -1.3 -21.8 65.4 -18 ~ -2.5 -8.8 CATCACTAATGTCAAACATG

4584 SEQ ID N0:6957 -1.3 -17.7 55 -15.2 -1.1 -5 CCTGACAGTCACTCAAATTG

4608 SEQ ID N0:6958 -1.3 -21.6 63.6 -20.3 0 -7.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATCTCGTGAATCAACAGCTG

4745 SEQ ID N0:6959 -1.3 -21.6 63.7 -19.7 0 -8.6 CCGGGACAAAACTAGGGTCA

5132 SEQ ID N0:6960 -1.3 -24.1 66.5 -21.5 -1.2 -7.1 AAGACGGATCACTCGGAACA

5327 SEQ ID N0:6961 -1.3 -22.1 62.6 -20.1 -0.4 -5.3 AATCCATCCCAGCCAGCAGA

5593 SEQ ID N0:6962 -1.3 -29.1 78 -27.8 0 -4.6 AAAGAAAATCCCAACAGATG

5702 SEQ ID NO:6963 -1.3 -l7 52 -15.7 0 -3 CGCGATGTACATGTTCACCA

5756 SEQ ID NO:6964 -l.3 -25.1 69.3 -23.2 0 -8.4 ATCCATGGCAATAAGCTGGA

5966 SEQ ID N0:6965 -1.3 -23.6 67 -19.8 -2.5 -8 ATAAAATATCAAGACAGTGG

6012 SEQ ID N0:6966 -1.3 -15.6 50.7 -14.3 0 -4.6 ATCACAAAGATAATTCTTTG

6482 SEQ ID N0:6967 -1.3 -15.8 51.6 -10.5 -4 -9.6 ACAAATTTTGTAAAAATATG

6827 SEQ ID N0:6968 -l.3 -11.6 42.6 -7.7 -2.6 -7.8 GGACCATGGAAATGCACTAG

6848 SEQ ID N0:6969 -1.3 -22.4 64 -19.7 -1.3 -8.8 AAACAAACATAACAGACTAA

7081 SEQ ID N0:6970 -1.3 -13.6 46.1 -12.3 0 -2 AGAAACAAACATAACAGACT

7083 SEQ 2D N0:6971 -l.3 -15.2 49.3 -13.9 0 -1.8 GATAGAAACAAACATAACAG

7086 SEQ ID N0:6972 -1.3 -13.8 46.6 -12.5 0 -1.2 AAATATTAGAGGAGACTTTA

7124 SEQ ID N0:6973 -1.3 -16.1 52.6 -13.9 -0.7 -7.1 AGCTCTGAAACTTAGAATAA

7191 SEQ ID N0:6974 -1.3 -l7 53.9 -15.7 0 -4.3 GATGGCAAAGCAATACTGCA

7316 SEQ ID N0:6975 -l.3 -22 63.2 -18.2 -2.5 -8.1 CTATGTTTAATGAATGATAC

7519 SEQ ID N0:6976 -1.3 -15.4 50.8 -14.1 0 -2.5 ACTATGTTTAATGAATGATA

7520 SEQ ID N0:6977 -1.3 -15.4 50.8 -14.1 0 -2.5 GTGTGTTTCCTTGAAATCCA

7756 SEQ ID N0:6978 -1.3 -23.5 68.5 -21.7 -0.1 -3.8 CAGTGTGTTTCCTTGAAATC

7758 5EQ ID N0:6979 -1.3 -21.5 65 -19.4 -0.6 -5.1 ACCAATGTATCTCCTATTGG

8076 SEQ ID N0:6980 -1.3 -22.7 66.3 -19.1 -2.3 -7.1 TAAACTTTTGGTTTGTGCAA

8333 SEQ ID NO:6981 -1.3 -19.3 59 -16.1 -1.3 -11.8 TCTGTGGATAAGAAAACAAA

44 SEQ ID N0:6982 -1.2 -15.3 49.6 -14.1 0 -2.9 TGCTGAATAAAGAATGTACC

846 SEQ ID N0:6983 -1.2 -17.9 54.9 -16.7 0 -4.2 ATCATCACATCAGAAAGCTT

1207 SEQ ID N0:6984 -1.2 -20.2 61.4 -19 0 -6.8 GTCAGGATCATCACATCAGA

1213 SEQ ID N0:6985 -1.2 -22.9 69.3 -21.7 0.7 -4.5 ACGCTCAGACAGAACACAGT

1231 SEQ ID N0:6986 -1.2 -23.2 67 -22 0 -3.4 GCCCATCCAAAACATAAAAG

1443 SEQ ID N0:6987 -1.2 -20 57 -18.8 0 -2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GAGTCGAAATAGAGACAGGA

1589 SEQ ID N0:6988 -1.2 -19.8 59.8 -17.1 -1.4 -5 AGAAAATGACCAGGACAAAA

1680 SEQ ID N0:6989 -1.2 -16.6 51.3 -15.4 0 -4 TCTGAAGAACTTTCCAACAG

1912 SEQ TD N0:6990 -1.2 -19.7 59.3 -17.8 -0.4 -4.1 TGGTGGTGCCCTCTGGGGGA

2481 SEQ ID N0:6991 -1.2 -32.2 88.1 -28.8 -2.2 -8.7 AGTCAACACACTACTGAATT

2831 SEQ ID N0:6992 -1.2 -19.3 59.1 -17.6 -0.1 -3.2 CTGTGAAAATCCCAGTAAAG

2865 SEQ ID N0:6993 -1.2 -19.2 57.1 -18 0 -2.9 ATAGTAATAAGGATCCATGG

2909 SEQ ID N0:6994 -1.2 -19 58.2 -16.9 0 -9.5 GTGCCACCGTGGGAGCGTAC

3227 SEQ ID N0:6995 -1.2 -30.6 81.3 -28.2 -1.1 -9.1 TTCAAAGTCAGACTCTCCAA

3791 SEQ ID N0:6996 -1.2 -21.5 64.2 -20.3 0 -6.8 GTATTTAAGTTTTCAAAGTC

3802 SEQ ID N0:6997 -1.2 -16.9 55.5 -15.7 0 -2.7 CTTCAGTATTTAAGTTTTCA

3807 SEQ ID N0:6998 -1.2 -18.8 60.3 -17.6 0 -2.6 CTGCTGAACTCTTCAGTATT

3817 SEQ ID N0:6999 -1.2 -22.1 66.9 -18.2 -2.7 -10.1 ACTGCTGAACTCTTCAGTAT

3818 SEQ ID N0:7000 -1.2 -22.2 67.2 -18.3 -2.7 -10.1 AATGGAAACTTTTTAATACA

3988 SEQ ID N0:7001 -1.2 -14.8 49 -13.6 0 -2.5 GCTCAACAATACTGTAGCAG

4065 SEQ 2D N0:7002 -1.2 -21.5 64.2 -18.2 -2.1 -6.6 CTACCAGGCTAACCAAAGAA

4317 SEQ ID N0:7003 -1.2 -21.8 61.7 -19.2 -1.3 -5 GTAATGATTTGATGGCACCG

4362 SEQ 2D N0:7004 -1.2 -22.3 63.8 -21.1 0 -4 CACCCATGATGCTAAAGATC

4512 SEQ ID N0:7005 -1.2 -22.2 63.6 -21 0 -4.5 GGTTACAAAATCAAAGACCA

5024 SEQ ID N0:7006 -1.2 -18.2 55.1 -16.5 -0.2 -4.1 GCAATCCATCCCAGCCAGCA

5595 SEQ ID N0:7007 -1.2 -31 81.5 -29.8 0 -4.6 GCGATGTACATGTTCACCAC

5755 SEQ ID N0:7008 -1.2 -24.5 69.8 -22.7 0 -8.4 ACCTCATAGAACATCTCAAA

5839 SEQ ID N0:7009 -1.2 -19.7 59.3 -18.5 0 -2.8 CCTTTGTCTGATCATTTGAG

6954 SEQ ID N0:7010 -1.2 -22.1 66.7 -20.9 0 -6.2 ACAAATAAAGTTGATTATAT

7860 SEQ ID N0:7011 -1.2 -13.2 45.9 -11.5 -0.1 -3.7 AGTAAATAGTTTTGTAAAAA

8363 SEQ ID N0:7012 -1.2 -12.8 45.3 -10.4 -1.1 -4.3 AATAAAAAATGAAATTTATT

8462 SEQ ID N0:7013 -1.2 -8.7 37.3 -6.2 -1.2 -5.5 ATATAATGCATTTAAGTAAC

8869 SEQ ID N0:7014 -1.2 -14.8 49.5 -12.9 0 -8.8 TTAAAAATATGCATTACAAC

9015 SEQ ID N0:7015 -1.2 -13.4 46 -12.2 0 -6.8 AAGCCCAGCATCCAAGATGG

l69 SEQ ID N0:7016 -1.1 -26.5 72 -23.9 -1.4 -6.9 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TCCCTAGAAGAGATTCTTTG

326 SEQ ID N0:7017 -1.1 -21.7 64.9 -18.6 -2 -4.7 GTACCAACAGTGCCTGTGCC

474 SEQ ID N0:7018 -1.1 -28.9 79.4 -26.2 -1.5 -8.7 AGATTCTCTAGTAAAAAGGC

515 SEQ ID N0:7019 -1.1 -18.1 57 -l7 0 -4 AAGAGATTCTCTAGTAAAAA

518 5EQ ID N0:7020 -1.1 -15 50 -12.6 -1.2 -9.3 GATAGCAGCAAGAGATTCTC

527 SEQ ID N0:7021 -1.1 -21.6 65.9 -20.5 0 -7 CATTCTTTGTCCAGTCAGGA

921 SEQ ID N0:7022 -1.1 -24.7 73.8 -22.4 -1.1 -4.5 ATGCCATCACAATGACACTG

1056 SEQ ID N0:7023 -1.1 -22.4 64.3 -20.6 -0.4 -3.8 ATCCAAAACATAAAAGTGAC

1439 SEQ ID N0:7024 -1.1 -15.5 49.7 -14.4 0 -2.9 GACGTTAGAGCTGAAGGTCC

2446 SEQ ID N0:7025 -1.1 -24.6 71.1 -22.6 -0.7 -8.8 AAGTTCTTCCATTGTGTTGG

2609 SEQ ID N0:7026 -1.1 -22.9 69 -21.3 -0.1 -4.3 CTAGATTCTTCAAGTTCTTC

2620 SEQ ID N0:7027 -1.l -20.2 64.2 -18.6 -0.2 -3.6 ACATTTCTGTCTAGATTCTT

2630 SEQ ID N0:7028 -1.1 -20.7 65.2 -19.6 0 -6 CCATAACAATTAAATTCACA

2727 SEQ ID N0:7029 -1.1 -16.4 51.5 -15.3 0 -3.1 AACACACTACTGAATTGCTC

2827 SEQ ID N0:7030 -1.1 -20.1 60.5 -19 0 -3.6 CCAAAGAGCTGCATGCCGAC

3166 SEQ ID N0:7031 -1.1 -26.5 70.8 -24.4 -0.8 -9.1 GTCATCATTGATCTTGCAGA

3206 SEQ ID N0:7032 -1.1 -22.6 68.6 -20.8 -0.5 -6.4 CAATTCCCTTTTGCATTCTT

3498 SEQ ID N0:7033 -1.1 -23.7 68.4 -22.6 0 -5.1 TGGACATGCAGCTGTCTATC

3603 SEQ ID N0:7034 -1.1 -24.5 72.8 -21.5 -1.9 -10.5 GTTCTGACTCACTGCTGAAC

3828 SEQ ID N0:7035 -1.1 -23.3 69.4 -22.2 0 -4.2 ACAACATCAACTGTGCTTCC

3892 SEQ ID N0:7036 -1.1 -23 66.7 -21.2 -0.4 -4.1 GAATGGAAACTTTTTAATAC

3989 SEQ ID N0:7037 -1.1 -14.7 48.9 -13.6 0 -2.5 CCTTCTTCTGTACTTACTTG

4012 SEQ ID N0:7038 -1.1 -22.7 68.7 -21.6 0 -4.8 AACACAATGAAAGTCTCAAA

4093 5EQ ID N0:7039 -1.1 -15.8 50.6 -14.1 -0.3 -3.1 TCAGAGTGAAGAATGACCCA

4833 SEQ ID N0:7040 -1.1 -22.2 64.3 -21.1 0 -2.7 AGCTTCCTTTTTAACATAGG

5504 SEQ ID N0:7041 -1.1 -21.3 64.2 -20.2 0 -4.3 TATTAAGAATAGGTGCTAGC

5613 SEQ ID N0:7042 -1.1 -19 59.3 -17.4 0 -8 GGTTTTGCTATGAGAAGAGG

5938 SEQ ID N0:7043 -1.1 -21.4 64.9 -20.3 0 -3.6 AACGTAAACCTCATTTACAA

6906 SEQ ID N0:7044 -1.1 -18.1 54.7 -14.3 -2.7 -6.1 ATGTTTAATGAATGATACAG

7517 SEQ ID N0:7045 -1.1 -15.5 50.9 -14.4 0 -3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TATGTTTAATGAATGATACA

7518 SEQ ID N0:7046 -l.1 -15.2 50.2 -14.1 0 -2.9 TACAAAATGCTGACAAATAA

7872 SEQ ID N0:7047 -1.1 -14.3 47.3 -13.2 0 -3.6 CATGTGAACTTGATCCTTTG

8692 SEQ ID N0:7048 -1.1 -21.1 62.9 -20 0 -4.3 AAGGGAGTTAATTGAAATTG

8763 SEQ ID N0:7049 -1.1 -16 51.6 -14.9 0 -3.9 TCTTGAAAAAAAAGTTAATA

8939 SEQ ID N0:7050 -1.1 -11.1 41.7 -l0 0 -2.9 TAAAACAGAGCCTTATGAAT

201 SEQ ID N0:7051 -1 -17.5 54 -16.5 0 -3.2 ATAAAACAGAGCCTTATGAA

202 SEQ ID N0:7052 -1 -17.5 54 -16.5 0 -3.2 TTTTCTTGCTCCATAATCTC

224 SEQ TD N0:7053 -1 -22.2 67.2 -21.2 0 -3.6 TACGAATTACCTTAGATAGT

412 SEQ ID N0:7054 -1 -18.8 57.6 -17.8 0 -3.5 GGTACCAACAGTGCCTGTGC

475 SEQ ID N0:7055 -1 -28.1 78.5 -25.2 -1.5 -11.6 ATAGCAGCAAGAGATTCTCT

526 SEQ ID N0:7056 -1 -21.9 66.6 -19.8 -1 -9 TGGAGGAATGTCTCCATAAA

662 SEQ ID N0:7057 -1 -20.8 61.6 -16.6 -3.2 -8.3 GGGTTTAGTGGAGTTAAAAT

799 SEQ ID N0:7058 -1 -19.4 60 -18.4 0 -2.8 TATAGATTCCAGTGAATGTG

948 SEQ TD N0:7059 -1 -19.5 60.2 -17.6 -0.7 -5.3 GAAACGTAAAATCTTCTAAG

1008 SEQ ID N0:7060 -1 -14.7 48.5 -13.7 0 -5.3 ACTGAAATCCAGCCAGTTCC

1040 SEQ ID N0:7061 -1 -25.6 71.6 -23.4 -1.1 -6.2 CCCAGGTCCACAAACTCTGT

1081 SEQ ID N0:7062 -1 -27.3 74.8 -25.8 -0.2 -3.7 GTCTTTAAACCTGGAATGAC

1156 SEQ 2D N0:7063 -1 -20 60 -19 0 -5.1 GGATCATCACATCAGAAAGC

1209 SEQ ID N0:7064 -1 -21 63 -19.5 -0.1 -5.3 GACTGTCATCTCCAATGTAA

1422 SEQ ID N0:7065 -1 -21.9 65.3 -20.3 -0.3 -6.8 TGCCCATCCAAAACATAAAA

1444 SEQ ID N0:7066 -1 -20 56.8 -19 0 -3 TTTAGCACCTTTGGAACTCA

1943 SEQ ID N0:7067 -1 -23 67.2 -22 0 -4.1 CCAGCATGGCGGACATTTCT

2642 SEQ ID N0:7068 -1 -27.2 74.6 -25 -1.1 -7.4 TGTGAAAATCCCAGTAAAGA

2864 SEQ ID N0:7069 -1 -18.9 56.6 -17.9 0 -2.1 GATCTTGAGAACCATTTCTG

2885 SEQ ID N0:7070 -1 -20.9 62.9 -18.5 -1.3 -6.3 GCCTTCTTGGAAATAGTAAT

2921 SEQ ID N0:7071 -1 -20.5 61.3 -18.9 -0.3 -3.2 CAGGATTTTGCCAACTTGAA

3046 SEQ 2D N0:7072 -1 -21.7 62.9 -20.1 -0.3 -3.5 CCAACATGAAAACAATAAGG

3351 SEQ ID N0:7073 -1 -15.8 49.6 -14.8 0 -5.2 TGACCAACATGAAAACAATA

3354 SEQ ID N0:7074 -1 -16.1 50.4 -15.1 0 -5.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Tnter-total dupleac Tm target molecularmolecular of Positionoligo bindingformationDupleacstructureoligo oligo GATTACGTATTTTTCAACAC

3707 SEQ ID N0:7075 -1 -18.2 56.7 -17.2 0 -6.1 CTCAACAATACTGTAGCAGG

4064 SEQ ID N0:7076 -1 -20.9 62.6 -l9 -0.7 -5.3 CTGAGAAGGATCATGAACAC

4108 SEQ ID N0:7077 -1 -19.3 58.6 -17.6 -0.5 -6.9 AATGAATATATAGGTAAAGA

4208 SEQ ID N0:7078 -1 -13.6 46.8 -12.6 0 -3.9 ACACCCATGATGCTAAAGAT

4513 SEQ ID N0:7079 -1 -22 62.7 -21 0 -4.4 GCCTGACAGTCACTCAAATT

4609 SEQ ID N0:7080 -1 -23.4 67.8 -22.4 0 -7.1 ACAAAATCAAAGACCATTCC

5020 SEQ ID N0:7081 -1 -18.5 55.3 -17.5 0 -2.8 TAGTGAAGTAGTAGTGTCTG

5211 SEQ TD N0:7082 -1 -20.3 65.1 -19.3 0 -2.3 TCACTCGGAACAAGGTAGGG

5319 SEQ ID N0:7083 -1 -23.2 66.7 -22.2 0 -2.9 TTTTAACATAGGCAAAGTTG

5496 SEQ ID N0:7084 -1 -17.5 55.1 -16.5 0 -4 GGAACAAGCAGATCATGCTG

5562 SEQ ID N0:7085 -l -22.2 64.7 -18.4 -2.8 -10.6 AGTTCTCCAGGATGACCGCG

5772 SEQ ID N0:7086 -1 -27.7 75.3 -25.3 -1.3 -6.6 TTATAGTTACTTGATATATT

6235 SEQ ID N0:7087 -1 -15.8 52.8 -14.8 0 -4.3 AGACTAAAATACTTTCAAAA

7068 SEQ ID N0:7088 -1 -13.3 45.9 -12.3 0 -2.5 AACAGACTAAAATACTTTCA

7071 SEQ ID N0:7089 -1 -15.6 50.7 -14.6 0 -2.5 TCTAAAGCAATACTTTAGAT

7276 5EQ ID N0:7090 -1 -16.7 53.6 -11.5 -4.2 -9.9 TTACATAACATAGGGTATTT

7372 SEQ ID N0:7091 -1 -17.8 56.3 -16.8 0 -3.3 TTTACATAACATAGGGTATT

7373 SEQ ID N0:7092 -1 -17.8 56.3 -16.8 0 -3.3 GACAAATAAAGTTGATTATA

7861 SEQ ID N0:7093 -1 -13.8 47.1 -12.3 -0.1 -3.7 TAAAAAAAAACATCTATGAA

8155 SEQ ID N0:7094 -1 -9.9 39.4 -8.9 0 -3.8 ATGGCTAAAAAGAACAAAGT

8260 SEQ ID N0:7095 -1 -15.4 49.5 -14.4 0 -3.7 TGTGAACTTGATCCTTTGCA

8690 SEQ ID N0:7096 -1 -22.9 67 -21.9 0 -4.8 AGAATGTAACATTTATTAGC

8847 SEQ ID N0:7097 -1 -16.5 53.5 -14.6 -0.8 -6.5 TCCACTGAGAATAAGAGTTT

8987 SEQ ID N0:7098 -1 -19.7 60.2 -18.7 0 -2.8 ATATCCCTAGAAGAGATTCT

329 SEQ ID N0:7099 -0.9 -21.2 63.7 -18.2 -2.1 -6 TCACAGCACCTTTTTCCGGA

393 SEQ ID N0:7100 -0.9 -27 74.6 -25.6 0 -8 GCTCAAGGTCATAAATACAC

896 SEQ ID N0:7101 -0.9 -19.7 59.9 -18.8 0 -2.8 AGTCGAAATAGAGACAGGAA

1588 SEQ ID N0:7102 -0.9 -18.5 56.6 -16.1 -1.4 -5 CAAATTCACCAAATAAAATG

1706 SEQ ID N0:7103 -0.9 -13.6 45.7 -12.7 0 -3.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGTGAGTCTCTCCTGCTTTC

2284 SEQ ID N0:7104 -0.9 -26.2 80.1 -24.7 -0.3 -4.6 CACAGTGCTGTGCATCTTCC

2396 SEQ ID N0:7105 -0.9 -27 78.3 -24.3 -1.2 -11.5 AGTTCTTCCATTGTGTTGGT

2608 SEQ ID N0:7106 -0.9 -24.8 75.3 -23.2 -0.4 -3.7 TGGCAATGATCTTGAGAACC

2892 SEQ ID N0:7107 -0.9 -21.7 63.3 -20.1 -0.4 -6.2 TTACCAAAGAGCTGCATGCC

3169 SEQ ID N0:7108 -0.9 -24.9 69.4 -23 -0.8 -9.1 ATGAAAACAATAAGGCACAT

3346 SEQ ID N0:7109 -0.9 -16.3 51.1 -15.4 0 -4 GACCATGACCAACATGAAAA

3359 SEQ ID N0:7110 -0.9 -19.7 56.9 -16.5 -2.3 -5.9 TTCATTGTCATCATCAGTAG

3452 SEQ ID N0:7111 -0.9 -20.7 65.3 -19.8 0 -2.4 CTGCAATCTGCAGATTATTC

3474 SEQ ID N0:7112 -0.9 -21.4 64.5 -17 -3.5 -14 CACTGCTGAACTCTTCAGTA

3819 SEQ ID N0:7113 -0.9 -22.9 68.4 -19.4 -2.6 -7.7 ACATCAACTGTGCTTCCTTC

3889 SEQ ID N0:7114 -0.9 -24.2 71.1 -23.3 0 -3.9 GCAACCCATTTGAGAAGCAT

4234 SEQ ID N0:7115 -0.9 -23.9 66.9 -22.3 -0.4 -4.6 AGCAACCCATTTGAGAAGCA

4235 SEQ ID N0:7116 -0.9 -23.9 67.1 -22.3 -0.4 -4.6 CCATGATGCTAAAGATCAAC

4509 SEQ ID N0:7117 -0.9 -19.5 58.1 -17.8 -0.6 -5.4 AACAATGAACACTAGGTTGA

5153 SEQ ID N0:7118 -0.9 -18.2 56 -16.5 -0.6 -6 GGTAGGGGACACAGAATACT

5306 SEQ ID N0:7119 -0.9 -22.8 67 -21.3 -0.3 -3.2 ACAAGGTAGGGGACACAGAA

5310 SEQ ID N0:7120 -0.9 -22.2 64.9 -21.3 0.5 -2.1 ATTCGGCCAATCCTGGCAAG

5344 SEQ ID N0:7121 -0.9 -26.6 71.8 -22.2 -3.5 -10.3 ATCCCCTTTGCTCCTTTGAT

5374 SEQ ID N0:7122 -0.9 -28.3 78 -27.4 0 -3.6 AACTGACAAAAAAGAAAATC

5712 SEQ ID N0:7123 -0.9 -11.3 41.8 -10.4 0 -2.2 AATTCTTTGTTTCTTTTTAC

6471 SEQ ID N0:7124 -0.9 -17.9 58.2 -17 0 -2.3 ACAAAGATAATTCTTTGTTT

6479 SEQ ID N0:7125 -0.9 -16.1 52.5 -10.5 -4.7 -11 AACATAACAGACTAAAATAC

7076 SEQ ID N0:7126 -0.9 -13.3 45.8 -12.4 0 -2 GAGATACCATATGATACTCA

7734 SEQ ID N0:7127 -0.9 -20 60.7 -19.1 0 -6.6 AATATATTATAACAAAGAAA

8046 SEQ ID N0:7128 -0.9 -10.2 40 -9.3 0 -5.3 GACTAGTCTGCATACGTAAA

8191 SEQ ID N0:7129 -0.9 -20.6 61.7 -18.9 0 -9.4 ATAAGACTAGTCTGCATACG

8195 SEQ ID N0:7130 -0.9 -20.1 60.9 -17.5 0 -11.5 AATAGTTTTGTAAAAAGAAT

8359 SEQ ID N0:7131 -0.9 -12.5 44.6 -10.3 -1.2 -4.3 AATAGCAATTCCCTGCATGC

8401 SEQ ID N0:7132 -0.9 -24.5 68.8 -21.9 -1.7 -8.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TTTTTGACCACAGAGGTTTA

95 SEQ ID N0:7133 -0.8 -21.7 65.3 -19.3 -1.5 -7.1 TCTGGCAGCTGTTCAGCTTT

128 SEQ ID N0:7134 -0.8 -27 80 -24.3 -1.8 -Z1 TATGAATTACAGCATAACAA

188 SEQ ID N0:7135 -0.8 -16 51.3 -15.2 0 -4.1 AAGAGATTCTTTGCTCCTTT

319 SEQ ID N0:7136 -0.8 -22.3 67.1 -20.8 -0.4 -5.3 TGGGGGTACCAACAGTGCCT

479 SEQ ID N0:7137 -0.8 -28.7 78.5 -25.6 -2.3 -8.8 TCAGGTCCTGGGGGTACCAA

487 SEQ ID NO:7138 -0.8 -28.7 79.9 -24.6 -3.3 -12.6 AGCAGCAAGAGATTCTCTAG

524 SEQ ID N0:7139 -0.8 -21.9 66.8 -19.8 -1.2 -9.3 AGAATGTACCAAAATCTTGA

836 SEQ ID N0:7I40 -0.8 -17.6 54.5 -16.2 -0.3 -4 GAATGTGTACTCTACATTCT

935 SEQ TD N0:7141 -0.8 -20.4 63.2 -16.4 -3.2 -11.2 GAATGACTGAAATTGTTTTC

1143 SEQ ID N0:7142 -0.8 -16.8 53.8 -14.9 -1 -3.6 CTGGATCAGGGCCCCCACAA

1178 SEQ ID NO:7143 -0.8 -30.9 80.1 -28.9 -0.3 -10.4 AAACACGCTCAGACAGAACA

1235 SEQ ID N0:7144 -0.8 -20.6 59.8 -19.8 0 -3.1 AGACAGGAAAGCCCAGCTAA

1577 SEQ ID N0:7145 -0.8 -24.1 67.3 -22.4 -0.8 -5 GTCGAAATAGAGACAGGAAA

1587 SEQ ID N0:7146 -0.8 -17.8 54.7 -I6.2 -0.6 -5 GCCAGGATCAAATTCACCAA

1714 SEQ ID N0:7147 -0.8 -23.4 65.6 -22 -0.3 -4.5 ACAGCTCTCCTAACCCACCT

1896 SEQ ID N0:7148 -0.8 -29.2 78.6 -28.4 0 -4.4 TTTCCAACAGCTCTCCTAAC

1902 SEQ ID N0:7149 -0.8 -24 69.2 -23.2 0 -4.4 AAAGCTGTCTCTCTCTCCTT

2021 SEQ ID N0:7150 -0.8 -25.1 74.8 -24.3 0 -5.1 AAAGTCATTTTCAGATCCAA

2231 SEQ ID N0:7151 -0.8 -19.3 59.1 -18.5 0 -4.5 ATTCTTCAAGTTCTTCCATT

2616 SEQ ID NO:7152 -0.8 -21.8 66.8 -21 0 -2.9 TCTTGGAAATAGTAATAAGG

2917 SEQ ID N0:7153 -0.8 -15.9 51.7 -15.1 0 -2.8 CCAGCCTTCTTGGAAATAGT ' 2924 SEQ ID N0:7154 -0.8 -24.2 69 -22.8 -0.3 -4.9 TACCAAAGAGCTGCATGCCG

3168 SEQ ID N0:7155 -0.8 -25.6 69.1 -23.8 -0.8 -9.1 TCCACTCTCCACACAGCACG

3285 SEQ ID N0:7156 -0.8 -27.9 76.1 -27.1 0 -4.1 TTCTGACTCACTGCTGAACT

3827 SEQ ID N0:7157 -0.8 -23 68 -22.2 0 -3.6 ATGAATATATAGGTAAAGAC

4207 SEQ ID N0:7158 -0.8 -14.5 48,g -13.7 0 -4.2 CATGATGCTAAAGATCAACC

4508 SEQ ID N0:7159 -0.8 -19.5 58,1 -17.9 -0.6 -5.4 AATTGTTAACATCACTAATG

4593 SEQ ID N0:7160 -0.8 -15.8 51.4 -14.3 -0.3 -8.3 TTGCCAAGAGCCTGACAGTC

4618 SEQ ID N0:7161 -0.8 -26.3 74.5 -24.7 -0.6 -5.7 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGCTTGCTTGCCAAGAGCCT

4625 SEQ ID N0:7162 -0.8 -28.7 79.3 -25.9 -2 -7.4 AAGATGATAAAGATGACAAA

4813 SEQ ID N0:7163 -0.8 -13.5 46.1 -12.7 0 -2.5 GAATGACCCAAAGATGATAA

4823 SEQ ID N0:7164 -0.8 -18.2 54.5 -17.4 0 -2.1 TGATATCAAAGACTTGTCTG

5043 SEQ ID N0:7165 -0.8 -18.3 57.5 -15.7 -1.4 -11.5 GAACACTAGGTTGATCCGGG

5147 SEQ ID N0:7166 -0.8 -24.3 68.6 -22.3 -1.1 -7.4 TATCATCTCAGCCAGAAACA

5282 SEQ ID N0:7167 -0.8 -21.8 64.2 -21 0 -3.1 CAGGAAGGGACATCATCAAA

5412 SEQ ID N0:7168 -0.8 -20.5 60.6 -19.1 -0.3 -2.9 AGGTGCTAGCAATCCATCCC

5603 SEQ ID N0:7169 -0.8 -28.1 77.9 -25.6 -0.2 -11.5 GTGTCAGGGTCACAGTCGGG

5641 SEQ ID N0:7170 -0.8 -27.9 81.5 -26.2 -0.7 -4.4 TGCCAGGGTGAATTGTGTCA

5655 SEQ ID N0:7171 -0.8 -25.6 73.7 -24.8 0 -3.8 AAGTTCTCCAGGATGACCGC

5773 SEQ ID N0:7172 -0.8 -26.2 73.1 -24 -1.3 -5.6 AGACAGTGGATCCGGTCACC

6001 SEQ ID N0:7173 -0.8 -27.4 76.5 -25 -1.1 -10.8 GTTATAGTTACTTGATATAT

6236 SEQ ID N0:7174 -0.8 -16.9 55.6 -16.1 0 -4.1 ACAGTCAGTTTGGCATGGAC

6559 SEQ ID N0:7175 -0.8 -24.3 72.4 -22.8 -0.5 -5.4 TTACAAAAATAGTGACATAG

6892 SEQ ID NO:7176 -0.8 -14 47.5 -13.2 0 -3.5 GAAACTGTAGCCATTACAAT

7019 SEQ ID N0:7177 -0.8 -19.7 58.8 -18 -0.7 -3.7 TAATTGACATAGACTATCAG

7353 SEQ ID N0:7178 -0.8 -16.8 54.1 -16 0 -3.3 ATAACTATTTACATAACATA

7380 SEQ ID N0:7179 -0.8 -14.2 48.2 -13.4 0 -1.7 AATGAATGATACAGGATACA

7511 SEQ ID N0:7180 -0.8 -16.8 53.1 -16 0 -2.4 ATACTACAAAAGTTGAATAA

8172 SEQ ID N0:7181 -0.8 -13.5 46.4 -12 -0.4 -4.2 CATACGTAAATACTACAAAA

8181 SEQ ID N0:7182 -0.8 -14.2 47.1 -13.4 0.1 -6.6 TTGTGCAAAP.AACAAAAATT

8321 SEQ ID N0:7183 -0.8 -12.5 43.8 -11 -0.5 -5.4 AACAAAAACAAAAAACCCCA

8502 SEQ ID N0:7184 -0.8 -15.7 48.3 -14.9 0 0 TAACAAAGAATGTAACATTT

8853 SEQ ID N0:7185 -0.8 -13.7 46.6 -12 -0.8 -7.2 CACTGAGAATAAGAGTTTGG

8985 SEQ ID N0:7186 -0.8 -18.5 57.5 -17.7 0 -2.8 CTGGCAGCTGTTCAGCTTTT

127 SEQ ID N0:7187 -0.7 -26.7 78.5 -24.3 -l.6 -10.8 CTAACAGGGTTTAGTGGAGT

805 SEQ ID N0:7188 -0.7 -22.5 68.2 -21.8 0 -3.6 TGCTCAAGGTCATAAATACA

897 SEQ ID N0:7189 -0.7 -19.5 59.3 -18.8 0 -3.6 AATGACACTGAAATCCAGCC

1046 SEQ ID N0:7190 -0.7 -22 62.7 -20.1 -1.1 -4 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAACTCTGTCACATATGCCA

1070 SEQ ID N0:7191 -0.7 -22.4 65.2 -21.7 0 -6.5 GACTGGATCAGGGCCCCCAC

1180 SEQ ID N0:7192 -0.7 -31.7 83.5 -29.6 -0.7 -10.5 CATTTTCAGATCCAACATCC

2226 SEQ ID N0:7193 -0.7 -22.4 65.4 -21.2 -0.1 -4.5 GCCCTGGCACCATCCTGGAT

2364 SEQ ID N0:7194 -0.7 -32.4 84.6 -29.9 -1.8 -10.4 AACCACACCATTGCAATCCA

2414 SEQ ID N0:7195 -0.7 -25 67.8 -23.7 0 -8.6 AATCTGGTAAGAGCTTAACC

2525 SEQ ID N0:7196 -0.7 -20.6 61.8 -19.2 -0.5 -6.3 ATTTAAGACAATGCAAATAG

2771 SEQ ID N0:7197 -0.7 -14.8 48.9 -13.5 -0.3 -5.6 AGTGTGGGCCAGGATTTTGC

3055 SEQ ID N0:7198 -0.7 -27.2 78.3 -26 0 -7.6 CTAGAGCCCCCACAGAATTG

3099 SEQ ID N0:7199 -0.7 -26.6 72 -25.9 0 -3.1 ATGACCATGACCAACATGAA

3361 SEQ ID N0:7200 -0.7 -21.1 60.3 -18.6 -1.8 -5.4 ATTCATTTCATTGTCATCAT

3458 SEQ ID N0:7201 -0.7 -20 62.7 -19.3 0 -2.4 CTTCCTACTGCAATCTGCAG

3481 SEQ ID N0:7202 -0.7 -24.7 71.2 -20 -4 -10.9 AAATCCATAAGCAACCCATT

4244 SEQ ID N0:7203 -0.7 -21.5 60.7 -20.8 0 -4.1 CAACCAGAAGATGAGACAGA

4493 5EQ ID N0:7204 -0.7 -20.1 59.6 -19.4 0 -2.4 TTGTTAACATCACTAATGTC

4591 SEQ ID N0:7205 -0.7 -18.1 57.3 -15.8 -1.5 -9.5 ATTGTTAACATCACTAATGT

4592 SEQ TD N0:7206 -0.7 -17.7 56 -15.6 -1.3 -9.5 GCATACATAATATCCATCCA

4726 SEQ ID N0:7207 -0.7 -22 63.8 -21.3 0 -3.4 AAATCAAAGACCATTCCTTG

5017 SEQ ID N0:7208 -0.7 -19.3 57.3 -18.6 0 -2.8 TCAAAGACTTGTCTGGTTAC

5038 SEQ TD N0:7209 -0.7 -20.4 62.9 -18.2 -1.4 -7 AAGAATAGGTGCTAGCAATC

5609 SEQ ID N0:7210 -0.7 -19.9 60.7 -17.8 0 -10.8 CACTATTAAGAATAGGTGCT

5616 SEQ ID N0:7211 -0.7 -19.3 59.3 -16.9 -1.7 -6.7 AGTCGGGTGGTGCACTATTA

5628 5EQ TD N0:7212 -0.7 -25.5 74.3 -23.5 -1.1 -9.5 GATGTAACTGACAAAAAAGA

5717 SEQ TD N0:7213 -0.7 -14.5 47.9 -13 -0.6 -3.4 TGCTATGAGAAGAGGAGGAT

5933 SEQ ID N0:7214 -0.7 -21.1 63.5 -20.4 0 -3.6 AAGGCAAATAAAATATCAAG

6019 SEQ ID N0:7215 -0.7 -13.3 45.6 -12.6 0 -4 ACACGCTTTGTAAAGGCAAA

6031 SEQ ID N0:7216 -0.7 -20.6 59.7 -17.9 -2 -6.4 TCTCCACTCTCACACAAAAC

6049 SEQ ID N0:7217 -0.7 -21.8 63.8 -21.1 0 0 TACTTGATATATTTTTTAAC

6228 SEQ TD N0:7218 -0.7 -14.6 49.7 -13.9 0 -4.3 TTCTTTGTTTCTTTTTACTT

6469 SEQ ID N0:7219 -0.7 -19,6 62.9 -18.9 0 -2.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total dupleac Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo CTTGTCAACGTTGATACCCT

6653 SEQ ID N0:7220 -0.7 -24.3 68.4 -21.3 -0.1 -12.8 AACAAATTTTGTAAAAATAT

6828 SEQ TD N0:7221 -0.7 -10.9 41.2 -8.5 -1.7 -8.7 ATAACAGACTAAAATACTTT

7073 SEQ ID N0:7222 -0.7 -14.2 47.9 -13.5 0 -2 ATGGCAAAGCAATACTGCAG

7315 SEQ ID N0:7223 -0.7 -21.4 62.2 -18.2 -2.5 -9.3 ATTTACATAACATAGGGTAT

7374 SEQ ID N0:7224 -0.7 -17.7 56 -l7 0 -3.3 TTGTAAATAGCTTCAAATTA

7683 SEQ ID N0:7225 -0.7 -15.6 51.7.-14.9 0 -4.6 AGTTTGGAATTGTGATTAAA

7959 SEQ ID N0:7226 -0.7 -16.8 53.8 -16.1 0 -3.2 AAGTTTGGAATTGTGATTAA

7960 SEQ ID N0:7227 -0.7 -16.8 53.8 -16.1 0 -3.1 GTTTGTGCAAAAAACAAAAA

8323 SEQ ID N0:7228 -0.7 -13.7 46 -11 -2 -9.4 GCATTACAACTGGAGTTTTC

9005 SEQ ID N0:7229 -0.7 -21.3 64.7 -20.6 0 -5.2 TAGATCCAAATTGTCTTTAA

9031 SEQ ID N0:7230 -0.7 -17.8 55,8 -17.1 0 -4.6 TTCCTCTGGCAGCTGTTCAG

132 SEQ ID N0:7231 -0.6 -27.5 80.5 -26.3 0 -8.5 TCCATAATCTCTGATAAAAC

215 SEQ ID N0:7232 -0.6 -17.1 53.8 -16.5 0 -3.3 CCACTCTACTATGAATCCTT

290 SEQ ID N0:7233 -0.6 -22.9 66.5 -22.3 0 -2.2 CAGTAAGCCACTCTACTATG

297 SEQ ID N0:7234 -0.6 -22.3 66.1 -21.7 0 -4.7 CGAATTACCTTAGATAGTCA

410 SEQ ID N0:7235 -0.6 -20 60.2 -19.4 0 -3.2 ACATTCTTTGTCCAGTCAGG

922 SEQ ID N0:7236 -0.6 -24.3 73 -23.7 0 -3.8 CAAAGGTATAGATTCCAGTG

954 SEQ ID N0:7237 -0.6 -20.1 61 -18.8 -0.4 -3.6 TCAGGGCCCCCACAATGGTC

1173 SEQ ID N0:7238 -0.6 -31 82.1 -28.6 -0.7 -11.8 AAGGTCCACCCACCAAGGAA

2433 SEQ ID N0:7239 -0.6 -27 71.7 -25.2 -1.1 -5.8 CATGGCGGACATTTCTGTCT

2638 SEQ ID N0:7240 -0.6 -25.2 72.2 -21.9 -2.7 -8.6 AGCATGGCGGACATTTCTGT

2640 SEQ ID N0:7241 -0.6 -25.7 73.1 -23.9 -1.1 -6.5 TCCATAACAATTAAATTCAC

2728 SEQ ID N0:7242 -0.6 -16.1 51.4 -15.5 0 -3.1 AAAAATGAAGACGATGATGG

3140 SEQ ID N0:7243 -0.6 -15.1 48.5 -14.5 0 -3.5 CAAAGAGCTGCATGCCGACC

3165 SEQ ID N0:7244 -0.6 -26.5 70.8 -24.9 -0.8 -9.1 ACCAAAGAGCTGCATGCCGA

3167 SEQ ID N0:7245 -0.6 -26.5 70.8 -24.9 -0.8 -9.1 CATGAAAACAATAAGGCACA

3347 SEQ ID N0:7246 -0.6 -17 52.3 -16.4 0 -4 CAACCCATTTGAGAAGCATT

4233 SEQ ID N0:7247 -0.6 -22.2 63.3 -21.6 0 -4.1 GATGAGACAGACCAACAGCA

4484 SEQ ID N0:7248 -0.6 -22.9 65.8 -22.3 0 -4.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AAGATGAGACAGACCAACAG

4486 SEQ ID N0:7249 -0.6 -19.7 58.9 -19.1 0 -2.4 CTGCATACATAATATCCATC

4728 SEQ ID N0:7250 -0.6 -20.2 60.7 -19.6 0 -4.9 AGAATGACCCAAAGATGATA

4824 SEQ ID N0:7251 -0.6 -18.9 56.4 -18.3 0 -2.1 CCAGTGAACAGAACAATGAA

5164 SEQ TD N0:7252 -0.6 -18.7 55.9 -17.4 -0.5 -3.9 GAAACATACCTACAATGGAG

5268 SEQ ID N0:7253 -0.6 -18.6 56.1 -17.5 -0.1 -4 CTATOATCTCAGCCAGAAAC

5283 SEQ ID N0:7254 -0.6 -22 65 -21.4 0 -3.6 TCTATCATCTCAGCCAGAAA

5284 SEQ ID NO:7255 -0.6 -22.2 65.9 -21.6 0 -3.6 AATTGTGTCAGGGTCACAGT

5645 SEQ ID N0:7256 -0.6 -23.7 71.7 -21.5 -1.6 -5.8 CAAATAAAATATCAAGACAG

6015 SEQ ID N0:7257 -0.6 -12.5 44.1 -11.9 0 -2.8 AAACACGCTTTGTAAAGGCA

6033 SEQ ID N0:7258 -0.6 -20.6 59.7 -18 -2 -5.8 AAAAGATAACATCTGAAATT

6199 SEQ ID NO:7259 -0.6 -13 45.2 -11.5 -0.8 -4.4 TAAAAGATAACATCTGAAAT

6200 SEQ ID N0:7260 -0.6 -12.6 44.4 -11.1 -0.8 -4.2 TCTTGTATAGGCACTGACTA

6594 SEQ ID N0:7261 -0.6 -22.2 67.4 -20.9 -0.4 -4.8 CTAAAATACTTTCAAAATTA

7065 SEQ ID N0:7262 -0.6 -12.3 44 -11.7 0 -3.2 GAATACTTTGCATAAAAATA

7151 SEQ ID N0:7263 -0.6 -14 47.2 -13.4 0 -5.1 TCTGAAACTTAGAATAAAAT

7188 SEQ ID N0:7264 -0.6 -12.9 45.2 -12.3 0 -2.3 GCTCTGAAACTTAGAATAAA

7190 SEQ ID N0:7265 -0.6 -16.3 52 -15.7 0 -2.8 CTATTTACATAACATAGGGT

7376 SEQ ID N0:7266 -0.6 -18.6 58 -18 0 -2.8 CATATTTGATTTAATAGAAG

7451 SEQ ID N0:7267 -0.6 -14 47.9 -13.4 0 -2.6 AATTAAAAGTGCAAAAGTAA

7709 SEQ ID N0:7268 -0.6 -12.8 44.7 -12.2 0 -5.4 TATGTACAAAATGCTGACAA

7876 SEQ ID N0:7269 -0.6 -16.9 52.9 -16.3 0 -6.8 TGATGTCATCAACCTGAAAA

7905 SEQ ID N0:7270 -0.6 -18.9 56.9 -17.5 -0.6 -6.2 ATTATCCATTGAAAAATCTT

7990 SEQ ID N0:7271 -0.6 -16.2 51.7 -14.9 -0.5 -3.4 ATAAAAAAAAACATCTATGA

8156 SEQ ID N0:7272 -0.6 -10.6 40.6 -10 0 -3.8 CGTAAATACTACAAAAGTTG

8177 SEQ ID N0:7273 -0.6 -14.9 48.7 -13.6 -0.4 -3.5 GTCAGATCAAGCACAGGTTT

8559 SEQ ID N0:7274 -0.6 -23.3 70 -22.7 0 -5.4 AAACAAAGAGACCTTTCCAG

31 SEQ ID N0:7275 -0.5 -19.7 58.2 -18.1 -1 -4.1 CTCTGGCAGCTGTTCAGCTT

129 SEQ ID N0:7276 -0.5 -27.8 81.7 -25.4 -1.8 -11 TAGCAGCAAGAGATTCTCTA

525 SEQ ID N0:7277 -0.5 -21.6 66 -19.8 -1.2 -9.3 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTATAGATTCCAGTGAATGT

949 SEQ ID N0:7278 -0.5 -20.7 63.5 -18.8 -1.3 -5.5 AAGGTATAGATTCCAGTGAA

952 SEQ ID N0:7279 -0.5 -20 61.1 -18.8 -0.4 -3.9 AAAGGTATAGATTCCAGTGA

953 SEQ ID N0:7280 -0.5 -20 61.1 -18.8 -0.4 -3.9 ACAATGACACTGAAATCCAG

1048 SEQ ID N0:7281 -0.5 -19.1 57.1 -17.6 -0.9 -4.9 TCATCACATCAGAAAGCTTC

1206 SEQ ID N0:7282 -0.5 -20.6 62.8 -20.1 0 -7 CCAAAACATAAAAGTGACTG

1437 SEQ ID N0:7283 -0.5 -16 50.3 -15.5 0 -3.1 ATCTCTTGAAGCAGCTGATG

1865 SEQ ID N0:7284 -0.5 -22.4 67.1 -21.2 0 -8.7 CCGACCTCTGAAACTGAAAA

2201 SEQ ID N0:7285 -0.5 -20.6 58.2 -20.1 0 -2.8 CACAAACAGTGAGTCTCTCC

2291 SEQ ID N0:7286 -0.5 -23.1 68.3 -21.7 -0.8 -4.8 GCATCTCCATTGAAATCTGG

2538 SEQ ID N0:7287 -0.5 -22.7 66 -22.2 0 -3.4 ACCATGACCAACATGAAAAC

3358 SEQ ID N0:7288 -0.5 -19.3 56.2 -16.5 -2.3 -5.9 TGACCATGACCAACATGAAA

3360 SEQ ID N0:7289 -0.5 -20.4 58.5 -17.6 -2.3 -5.9 TAAATGAACTCAATAATAAG

3414 SEQ ID N0:7290 -0.5 -11.7 42.8 -11.2 0 -3.9 ACCTACACCACTGGTGGTTC

3677 SEQ ID N0:7291 -0.5 -27 76.5 -24.4 -l.7 -12 TAGGTAAAGACTTTGTCAGC

4198 SEQ ID N0:7292 -0.5 -20.4 63.1 -18.6 -1.2 -5.2 TAACATCACTAATGTCAAAC

4587 SEQ ID N0:7293 -0.5 -16.2 52 -14.1 -1.5 -4.3 TCACTCAAATTGTTAACATC

4600 SEQ ID N0:7294 -0.5 -17.6 55.7 -16.6 0 -8.3 GTCGGGTGGTGCACTATTAA

5627 5EQ ID N0:7295 -0.5 -24.8 71.5 -23.4 -0.3 -9.5 TGAGAAGAGGAGGATCCAGG

5928 SEQ ID N0:7296 -0.5 -23 67.4 -19.9 -2.6 -9 CAATTGATCACAAAGATAAT

6488 SEQ ID N0:7297 -0.5 -14.9 48.9 -13.5 -0.8 -6.6 TGGTAATAAAAACAGCAACC

6674 SEQ ID N0:7298 -0.5 -17.2 52.7 -16.7 0 -4.1 CAAACATAACAGACTAAAAT

7078 SEQ ID N0:7299 -0.5 -13.4 45.6 -12.9 0 -2 AAAATTTGCACTTGCTGAAA

7173 SEQ ID N0:7300 -0.5 -17.7 54.2 -16.6 -0.3 -6.5 TCCAGAGAGATACCATATGA

7740 SEQ ID N0:7301 -0.5 -21.8 64.3 -21.3 0 -6 AATATGAAGAATAGGTTTTG

7793 SEQ ID N0:7302 -0.5 -15.2 50.3 -14.7 0 -2.7 CAAAATGCTGACAAATAAAG

7870 SEQ ID N0:7303 -0.5 -13.7 46 -13.2 0 -3.6 GCATGCAGTTCTTCAATACA

8387 SEQ ID N0:7304 -0.5 -22.7 67.6 -21.3 0 -9.5 CAAAAAACCCCAGA

8500 SEQ ID N0:7305 -0.5 -16.8 50.3 -16.3 0 0 GGTTTTATTAATTATATATA

8544 SEQ ID N0:7306 -0.5 -14.4 49.4 -13.9 0 -5.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo AGTTAATATAAATTCTCAAT

8927 SEQ ID N0:7307 -0.5 -14.1 48.2 -13.6 0 -3.1 TTTCCACTGAGAATAAGAGT

8989 SEQ ID N0:7308 -0.5 -19.7 60.2 -19.2 0 -2.9 TCAAAGGTATAGATTCCAGT

955 SEQ ID N0:7309 -0.4 -20.5 62.5 -20.1 0.4 -2.8 GACACTGAAATCCAGCCAGT

1043 SEQ ID N0:7310 -0.4 -24.6 69.2 -23 -1.1 -6.2 CACAATGACACTGAAATCCA

1049 SEQ ID N0:7311 -0.4 -19.8 58.1 -19.4 0 -3.1 ATCAGGGCCCCCACAATGGT

1174 SEQ ID N0:7312 -0.4 -30.6 80.3 -28.4 -0.7 -11.8 CATCAGAAAGCTTCTTTACC

1200 SEQ ID N0:7313 -0.4 -21.2 63.4 -19.9 -0.7 -7 GACAGAACACAGTCAGGATC

1224 SEQ ID N0:7314 -0.4 -21.7 65.4 -20.5 -0.6 -5.3 CATCCAAAACATAAAAGTGA

1440 SEQ ID N0:7315 -0.4 -16 50.4 -15.6 0 -2.9 AATGAGCCCAAGAAAATGAC

1690 SEQ ID N0:7316 -0.4 -l9 55.9 -18.6 0 -2.4 TTGTTTCCTTCAAGGTGCTC

1999 SEQ ID N0:7317 -0.4 -24.8 74.1 -23.6 -0.6 -5.3 GGTAAGAGCTTAACCTTCTC

2520 SEQ ID N0:7318 -0.4 -22.7 67.9 -21.7 -0.3 -7.2 ATCTCCATTGAAATCTGGTA

2536 SEQ ID N0:7319 -0.4 -21.1 63.2 -20.1 -0.3 -3.7 AATGGATCCATAACAATTAA

2734 SEQ ID N0:7320 -0.4 -16.5 51.8 -14.1 0 -12.2 AAATGGATCCATAACAATTA

2735 SEQ ID N0:7321 -0.4 -16.5 51.8 -14.1 0 -12.2 ATTTCTGCTGTGAAAATCCC

2872 SEQ ID N0:7322 -0.4 -22.3 64.6 -21 -0.7 -5 GCCAGAAAGAGGTTCAGAAC

3394 SEQ ID N0:7323 -0.4 -22 64.6 -20.3 -1.2 -7.1 AATGAACTCAATAATAAGGC

3412 SEQ ID N0:7324 -0.4 -15.7 50.6 -15.3 0 -3.9 GATTATTCATTTCATTGTCA

3462 SEQ ID N0:7325 -0.4 -19.3 61 -18.9 0 -2.4 AGATTATTCATTTCATTGTC

3463 SEQ ID N0:7326 -0.4 -18.6 59.9 -17.7 -0.2 -3 GTATTATTGGACATGCAGCT

3610 SEQ ID N0:7327 -0.4 -22.7 67.7 -22.3 0 -5.5 AGTATTTAAGTTTTCAAAGT

3803 SEQ ID N0:7328 -0.4 -16.5 54.3 -16.1 0 -2.4 AACTCTTCAGTATTTAAGTT

3811 SEQ ID N0:7329 -0.4 -18.3 58.7 -17.4 -0.2 -3.6 AAGTCTTCTTCGGGTTCAGT

3940 SEQ TD N0:7330 -0.4 -24.5 73.9 -24.1 0 -4.4 GACTTTGTCAGCATATTCTA

4190 SEQ ID N0:7331 -0.4 -21.5 66.3 -20.4 -0.4 -5.1 GTTTGAAATCCATAAGCAAC

4249 SEQ ID N0:7332 -0.4 -18.7 56.7 -18.3 0 -4.1 ATATGTTTGAAATCCATAAG

4253 SEQ ID N0:7333 -0.4 -16.4 52.4 -16 0 -3.5 GCTACCAGGCTAACCAAAGA

4318 SEQ ID N0:7334 -0.4 -24.3 67.5 -22.5 -1.3 -5 CAGAGTGAAGAATGACCCAA

4832 SEQ ID NO:7335 -0.4 -21.1 60.9 -20.7 0 -2.1 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ACAATGAACACTAGGTTGAT

5152 SEQ ID N0:7336 -0.4 -18.9 57.8 -17.7 -0.6 -6 AACAGAACAATGAACACTAG

5158 SEQ ID N0:7337 -0.4 -15.9 50.8 -15.5 0 -4 GCCAGAAACATACCTACAAT

5272 SEQ ID N0:7338 -0.4 -21.3 60.9 -20.9 0 -2 TTGTAATTTGGAACAAGCAG

5571 SEQ ID N0:7339 -0.4 -18.3 56.5 -16.3 -1.6 -7.1 TAACTGACAAAAAAGAAAAT

5713 SEQ ID N0:7340 -0.4 -10.6 40.5 -10.2 0 -2.2 CAGGAAGGATATGATGATGT

5732 SEQ ID N0:7341 -0.4 -19.7 59.9 -19.3 0 -2.6 ACCGCGATGTACATGTTCAC

5758 SEQ TD N0:7342 -0.4 -24.6 68.8 -23.6 0 -8.4 TGTAAAGGCAAATAAAATAT

6023 SEQ ID N0:7343 -0.4 -13.1 45.2 -12.7 0 -4 TGAAATTACGCTGAATGATA

6186 SEQ ID N0:7344 -0.4 -16.9 52.5 -16.5 0 -3.3 GTCAGTTTGGCATGGACCTC

6556 SEQ ID N0:7345 -0.4 -26.7 78 -25.8 -0.1 -5.4 TCTTGTCAACGTTGATACCC

6654 SEQ ID N0:7346 -0.4 -23.8 68.1 -21.3 0.4 -12.3 ACAGCAACCTCTTGTCAACG

6663 SEQ ID N0:7347 -0.4 -23.8 67.3 -22.8 -0.3 -4.6 GAACAACTATTCTAAAGCAA

7286 SEQ ID N0:7348 -0.4 -16.4 52 -16 0 -4.l GCAAAAGACACATACACTGT

7475 SEQ ID N0:7349 -0.4 -19.7 58.8 -19.3 0 -3.4 ATTTTTACATTATTTATATT

7842 SEQ ID N0:7350 -0.4 -14.5 49.7 -14.1 0 -2.2 CTGACAAATAAAGTTGATTA

7863 SEQ ID N0:7351 -0.4 -15 49.4 -14.1 -0.1 -3.3 TTATGTACAAAATGCTGACA

7877 SEQ ID N0:7352 -0.4 -17.7 54.9 -17.3 0 -6.8 CTGCCAACAGCAA<~AAACAA

8517 SEQ ID N0:7353 -0.4 -18.8 54.7 -16.9 -1.4 -4.2 TATAATGCATTTAAGTAACA

8868 SEQ ID N0:7354 -0.4 -15.5 50.8 -14.4 0 -8.8 CCAAATTGTCTTTAAAAATA

9026 SEQ ID N0:7355 -0.4 -14.7 48.4 -14.3 0 -4.3 GAGAATTATGAAATACATAT

9052 SEQ ID N0:7356 -0.4 -13.8 47.1 -12.3 -1 -4.6 AGAGATTCTTTGCTCCTTTC

318 SEQ ID N0:7357 -0.3 -23.4 71.2 -22.4 -0.4 -4.2 AATATCCCTAGAAGAGATTC

330 SEQ ID N0:7358 -0.3 -19.6 59.7 -17.9 -1.3 -5.2 ATTACCTTAGATAGTCACAG

407 SEQ ID N0:7359 -0.3 -20.2 62.5 -19.9 0 -2.7 TTGGCTTTCTCTTCTGCAGC

556 SEQ ID N0:7360 -0.3 -26.5 79.1 -25.4 -0.6 -8.3 ACACCATCTCTGGAGGAATG

672 SEQ ID N0:7361 -0.3 -23.3 67.4 -21.4 -1.5 -6.1 CCAGGTCCTCCAGGGGCTCT

693 SEQ ID N0:7362 -0.3 -33.1 90.9 -31.4 -1.3 -5.6 GTTAAAATATACAAGGCAGA

787 SEQ ID N0:7363 -0.3 -16.8 53.1 -16.5 0 -4 GTTTAGTGGAGTTAAAATAT

797 SEQ ID N0:7364 -0.3 -16.7 54.2 -16.4 0 -2.8 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo ATAAAGAATGTACCAAAATC

840 SEQ ID N0:7365 -0.3 -14.3 47.6 -14 0 -4.2 TGCCATCACAATGACACTGA

1055 SEQ ID N0:7366 -0.3 -23 65.6 -22 -0.4 -3.8 AGTTAAATGTGCTCATTGTT

1395 SEQ ID N0:7367 -0.3 -20 61.9 -18.5 -1.1 -3.5 CCATCCAAAACATAAAAGTG

1441 SEQ TD N0:7368 -0.3 -17.4 52.7 -17.1 0 -2.9 AAATAGAGACAGGAAAGCCC

1583 SEQ ID N0:7369 -0.3 -20.6 59.9 -19.6 -0.4 -3.2 CTCAAGAGAGACTGATGAGG

2134 SEQ ID N0:7370 -0.3 -20.6 62.5 -19.2 -1 -5.1 ATAACAATTAAATTCACAAG

2725 SEQ ID N0:7371 -0.3 -13 45.3 -12.7 0 -3.1 CAATGCAAATAGTGATGGCA

2763 SEQ ID N0:7372 -0.3 -20.3 59.9 -19.2 -0.6 -5.6 GCTGTGAAAATCCCAGTAAA

2866 SEQ ID N0:7373 -0.3 -21 60.7 -20.2 -0.1 -3.5 ATGGCAATGATCTTGAGAAC

2893 SEQ ID N0:7374 -0.3 -19.7 59.6 -18.7 -0.4 -6.2 CCATGGCAATGATCTTGAGA

2895 SEQ ID N0:7375 -0.3 -22.9 66 -21.9 -0.5 0 AAAGATATTCCAGCCTTCTT

2933 SEQ TD N0:7376 -0.3 -22.3 65.3 -21.3 -0.4 -4 CTTGCAGACACATTCTTTGT

3194 SEQ ID N0:7377 -0.3 -22.7 68 -21.9 0 -8.2 ATTGATCTTGCAGACACATT

3200 SEQ ID N0:7378 -0.3 -21.1 63.7 -20.8 0 -5.2 TCATTGTCATCATCAGTAGC

3451 5EQ ID N0:7379 -0.3 -22.4 69.6 -22.1 0 -2.8 ATCCATAAGCAACCCATTTG

4242 SEQ ID N0:7380 -0.3 -23 64.6 -22.7 0 -4.1 CCACTTGAAGCAGTGCAAGA

4692 5EQ ID N0:7381 -0.3 -23.9 68.1 -21.9 -1.7 -9.6 TTCAGAGTGAAGAATGACCC

4834 SEQ ID N0:7382 -0.3 -21.6 63.4 -20.5 -0.6 -3.9 GATGTTAAACAACGCAGGAA

5426 SEQ ID N0:7383 -0.3 -19 56.4 -18.2 -0.2 -4.7 GTAGCAACACTGAAGTTCTC

5785 SEQ ID N0:7384 -0.3 -21.6 65.8 -21.3 0 -6.3 TTACGCTGAATGATAGCGGC

6181 SEQ ID N0:7385 -0.3 -23.3 65.5 -19.4 -3.6 -9.4 TTGTTATAGTTACTTGATAT

6238 SEQ ID N0:7386 -0.3 -17.3 56.5 -17 0 -2.6 CAACGTAAACCTCATTTACA

6907 SEQ ID N0:7387 -0.3 -19.5 57.5 -16.5 -2.7 -6.1 AAACTTAGAATAAAATTTGC

7184 SEQ ID N0:7388 -0.3 -13 45.3 -12.7 0 -5.2 TCACCAATATGTGACATTCT

7621 SEQ ID N0:7389 -0.3 -21.1 62.9 -19.8 -0.9 -5.6 TAAAGGTGTTTGTAAATAGC

7692 SEQ ID N0:7390 -0.3 -17.1 54.7 -16.8 0 0 AGTGTGTTTCCTTGAAATCC

7757 SEQ ID N0:7391 -0.3 -22.8 67.6 -21.7 -0.6 -4.3 ATTATATTTTTACATTATTT

7847 SEQ ID NO:7392 -0.3 -14.5 49.7 -14.2 0 -2.2 ATAGTTTTGTAAAAAGAATT

8358 SEQ ID N0:7393 -0.3 -13.3 46.3 -11.6 -1.3 -9.3 kcal/

mot kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GCACAGGTTTTATTAATTAT

8549 SEQ ID N0:7394 -0.3 -18.7 58.6 -18.4 0 -4.2 AAAAAAAAGTTAATATAAAT

8934 SEQ TD N0:7395 -0.3 -7.4 35.1 -7.1 0 -2.8 CACATAAATCCAAGGGTTCT

8956 SEQ ID No:7396 -0.3 -21.3 62.4 -21 0 -4.3 ACTGAGAATAAGAGTTTGGT

8984 SEQ ID N0:7397 -0.3 -Z9 59.3 -18.7 0 -2.7 AGATCCAAATTGTCTTTAAA

9030 5EQ ID N0:7398 -0.3 -17.4 54.6 -17.1 0 -4.6 TCAGTGCTCGGAGAACTCTG

1125 SEQ ID N0:7399 -0.2 -24.3 70.8 -23.2 -0.7 -6.4 CAGTCAGGATCATCACATCA

1215 SEQ ID N0:7400 -0.2 -23 69.1 -22.3 -0.1 -5.3 AAATGTGCTCATTGTTACAT

1391 SEQ ID N0:7401 -0.2 -19.6 60 -17.8 -1.6 -4 CAGTTAAATGTGCTCATTGT

1396 SEQ ID N0:7402 -0.2 -20.6 62.8 -18.8 -1.6 -4 CATCTGCTGAAATTCGGCCT

1796 SEQ ID N0:7403 -0.2 -25.1 69.4 -23.5 -1.3 -9.5 GATTCTTCAAGTTCTTCCAT

2617 SEQ TD N0:7404 -0.2 -22.3 67.8 -22.1 0 -2.9 GACAATGCAAATAGTGATGG

2765 SEQ ID N0:7405 -0.2 -18.6 56.6 -17.5 -0.7 -5.1 TTTGCCAACTTGAAAACTCT

3040 SEQ ID N0:7406 -0.2 -20.1 59.3 -19.9 0 -3 CTATCCACTCTCCACACAGC

3288 SEQ ID N0:7407 -0.2 -26.8 76 -26.6 0 -2.8 CATTCTTCCTACTGCAATCT

3485 SEQ ID N0:7408 -0.2 -23.4 68.7 -23.2 0 -4.9 TGCATTCTTCCTACTGCAAT

3487 SEQ ID N0:7409 -0.2 -23.9 69.3 -22.2 -1.4 -5.3 GACATGCAGCTGTCTATCTT

3601 SEQ ID N0:7410 -0.2 -24.3 72.7 -22.8 -1.1 -9.6 CAAAGTCAGACTCTCCAACA

3789 SEQ ID N0:7411 -0.2 -21.9 64.2 -21.7 0 -7.3 ACCAGTTGTGCTCAACAATA

4074 SEQ ID N0:7412 -0.2 -22.4 65.6 -18.9 -3.3 -6.6 CATGAACACAATGAAAGTCT

4097 SEQ TD N0:7413 -0.2 -17.4 54 -17.2 0 -3.7 TGCCTTCAAACCGGGATAAG

4413 SEQ ID N0:7414 -0.2 -23.5 64.7 -23.3 0 -7.1 CATACATAATATCCATCCAG

4725 SEQ ID N0:7415 -0.2 -20.2 60 -20 0 -2.6 TCTCAGCCAGAAACATACCT

5277 SEQ ID N0:7416 -0.2 -23.8 67.7 -23.6 0 -2.7 GGCTGTAAACAATTGATCAC

6497 SEQ ID N0:7417 -0.2 -19.6 59.2 -19.4 0 -7.2 AACTTAGAATAAAATTTGCA

7183 SEQ ID N0:7418 -0.2 -14.4 48 -14.2 0 -5.2 CCTAAATTAAATATAGAGCT

7207 SEQ TD N0:7419 -0.2 -16.3 51.9 -16.1 0 -5.3 AAGATGGCAAAGCAATACTG

7318 SEQ ID N0:7420 -0.2 -18.8 56.6 -17.7 -0.7 -4.3 TACATAACATAGGGTATTTA

7371 SEQ ID N0:7421 -0.2 -17.4 55.4 -17.2 0 -3.1 TATTTACATAACATAGGGTA

7375 SEQ ID N0:7422 -0.2 -17.4 55.4 -17.2 0 -3.2 kcal/

mol kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo TATCACCAATATGTGACATT

7623 SEQ ID N0:7423 -0.2 -19.5 59.1 -17.8 -1.4 -6.5 GTGTTTCCTTGAAATCCAGA

7754 SEQ ID N0:7424 -0.2 -22.9 67 -21.9 -0.6 -4.3 AATTATCCATTGAAAAATCT

7991 SEQ ID N0:7425 -0.2 -15.4 49.8 -14.5 -0.5 -3.4 TGCTTATAGCCAAGGGAGTT

8774 SEQ ID N0:7426 -0.2 -24.3 70.7 -22.8 -1.2 -4.9 GAGATAGCATCAATTATATT

8813 SEQ ID N0:7427 -0.2 -17.4 55.5 -16.3 -0.7 -5,7 AAGTTAATATAAATTCTCAA

8928 SEQ ID N0:7428 -0.2 -13.4 46.6 -13.2 0 -3.1 TGAAATACATATTTAGATCC

9044 SEQ ID N0:7429 -0.2 -16.4 52.6 -15.6 -0.3 -5.8 CAAAGCCCAGCATCCAAGAT

172 SEQ ID N0:7430 -0.1 -25.3 68.6 -24.3 -0.7 -4.1 TTGATGTGGCTTGGCTTCAG

244 SEQ ID N0:7431 -0.1 -25 73.6 -24.2 -0.4 -6 GCAATTTTCCTAACAGGGTT

814 SEQ ID N0:7432 -0.1 -23 67 -22 -0.7 -4.6 AAATGAGCCCAAGAAAATGA

1691 SEQ ID N0:7433 -0.1 -18.1 53.8 -18 0 -3.2 GGATCAAATTCACCAAATAA

1710 SEQ ID N0:7434 -0.1 -17.2 53 -17.1 0 -5.3 CCTCTTTTTGTTCTGCTTCT

1779 SEQ ID N0:7435 -0.1 -25.3 75.9 -25.2 0 -3.6 ACTGAAATCTCTTGAAGCAG

1871 SEQ ID N0:7436 -0.1 -19.4 59.3 -18.8 -0.2 -4.1 GGAAAGCTGTCTCTCTCTCC

2023 SEQ ID N0:7437 -0.1 -25.9 76.5 -25.8 0 -6.4 TCTGTCTAGATTCTTCAAGT

2625 5EQ ID N0:7438 -0.1 -21.2 66.9 -20.6 -0.2 -6 AATTAAATTCACAAGATGTT

2720 SEQ ID N0:7439 -0.1 -15 49.6 -14.9 0 -3 CATAACAATTAAATTCACAA

2726 SEQ ID N0:7440 -0.1 -13.7 46.4 -13.6 0 -3.1 AGCGTACAGTCATCATTGAT

3214 SEQ ID N0:7441 -0.1 -22.5 67.2 -21.7 -0.5 -5.7 CCATGACCAACATGAAAACA

3357 SEQ ID N0:7442 -0.1 -19.8 56.9 -17.4 -2.3 -5.9 ATAATAAGGCCAGAAAGAGG

3402 SEQ TD N0:7443 -0.1 -18.7 56.5 -18.1 0 -7.7 GCAATCTGCAGATTATTCAT ' 3472 SEQ ID N0:7444 -0.1 -21.2 63.9 -18.2 -1.7 -14 GCAATTGGCACTGTGACGGT

3769 SEQ ID N0:7445 -0.1 -25.9 72.3 -24.9 -0.7 -7 TCACTGCTGAACTCTTCAGT

3820 SEQ ID N0:7446 -0.1 -23.6 70.7 -20.8 -2.7 -7,6 TTCTAGTTCTGACTCACTGC

3833 SEQ ID N0:7447 -0.1 -23.4 71.8 -22.6 -0.4 -4 CAAGCTTCCGGTTTAAAGTC

3955 SEQ ID N0:7448 -0.1 -22.5 65.4 -22.4 0 -6.6 ACAGAATGGAAACTTTTTAA

3992 SEQ ID N0:7449 -0.1 -15.7 50.8 -14.9 -0.4 -2.7 CACCAGGCATTAGTGAAATA

4270 SEQ ID N0:7450 -0.1 -21.2 62.2 -21.1 0 -4.2 GCACCAGGCATTAGTGAAAT

4271 SEQ ID N0:7451 -0.1 -23.3 66,7 -22.3 -0.7 -4.6 kcal/

mot kcal/moldeg kcal/molkcal/molkcal/mol C

Intra- Inter-total duplex Tm target molecularmolecular of Positionoligo bindingformationDuplexstructureoligo oligo GTTACAAAATCAAAGACCAT

5023 SEQ ID N0:7452 -0.1 -17 52.8 -16.9 0 -2.8 CAAGGTAGGGGACACAGAAT

5309 SEQ ID N0:7453 -0.1 -22 64.3 -21.3 -0.3 -2.8 ATTTGGAACAAGCAGATCAT

5566 SEQ ID N0:7454 -0.1 -19.7 59.6 -18 -1.6 -8.6 TCATAGGCTGTAAACAATTG

6502 SEQ ID N0:7455 -0.1 -18.5 57 -18.4 0 -6.7 CCTCAGCAGTGTCAGCTGGT

6690 SEQ ID N0:7456 -O.l -29.1 84.9 -26.2 -2.8 -9.2 GTAAACCTCATTTACAAAAA

6903 SEQ ID NO:7457 -0.1 -15.7 50.2 -13.4 -2.2 -5.9 AACTGAAACTGTAGCCATTA

7023 SEQ ID N0:7458 -O.l -19.9 59.4 -19.8 0 -3.3 GACTAAAATACTTTCAAAAT

7067 SEQ ID N0:7459 -O.l -13.3 45.9 -13.2 0 -2 TTACATTATTTATATTTAAA

7838 SEQ ID N0:7460 -0.1 -12.6 45.2 -12 -O.l -4.6 GATTCAAAAGTTTGGAATTG

7967 SEQ ID N0:7461 -0.1 -16.3 52.3 -15.3 -0.8 -7.1 AAATAGTTTTGTAAAAAGAA

8360 SEQ ID N0:7462 -O.l -11.8 43.1 -10.3 -1.3 -4.2 AAAGTTCACAAAGCTGCTTT

8664 SEQ ID N0:7463 -0.1 -20.1 60.5 -18.7 -0.1 -10.6 AAAACAAAGAGACCTTTCCA

32 SEQ ID N0:7464 0 -19 56.3 -18.1 -0.8 -4.4 CAGAGGTTTACAAATTAGTT

85 5EQ ID N0:7465 0 -18.2 57.4 -18.2 0 -3.3 TGAATGTGTACTCTACATTC

936 SEQ TD N0:7466 0 -19.5 61.1 -16.4 -3.1 -11 TGGAATGACTGAAATTGTTT

1145 SEQ ID N0:7467 0 -17.5 54.7 -17.5 0 -3.2 CTGGAATGACTGAAATTGTT

1146 SEQ ID N0:7468 0 -18.3 56.2 -18.3 0 -3.2 AACACGCTCAGACAGAACAC

1234 SEQ ID N0:7469 0 -21.5 62.2 -21.5 0 -3.4 AGATTGCCCATGAACAGCTG

1270 SEQ ID N0:7470 0 -24.7 69.2 -24.2 0.5 -8.6 GACAGGAAAGCCCAGCTAAA

1576 SEQ ID N0:7471 0 -23.4 65.1 -22.4 -0.9 -6 AGCCAGGATCAAATTCACCA

1715 SEQ ID N0:7472 0 -24.1 67.9 -24.1 0.5 -5.3 CATCAGCAAAGTCATTTTCA

2238 SEQ ID N0:7473 0 -20.6 62.6 -20.6 0 -3.3 AAGCCCTGGCACCATCCTGG

2366 SEQ ID N0:7474 0 -31.1 81.2 -30 -0.8 -9.6 AAGGAAACCACACCATTGCA

2419 SEQ ID N0:7475 0 -23 63.8 -22.3 -0.5 -5.3 CAGTCAACACACTACTGAAT

2832 SEQ ID N0:7476 0 -19.9 60 -l9 -0.8 -4.2 TTGACAGAATGGAAACTTTT

3995 SEQ ID N0:7477 0 -17.3 54.2 -16.6 -0.4 -3.2 AATATGTTTGAAATCCATAA

4254 SEQ ID N0:7478 0 -15.7 50.6 -15.7 0 -3.5 TTAATGTCCGTAATGATTTG

4371 SEQ ID N0:7479 0 -18.5 56.6 -18.5 0 -2.6 TTGAAGCAGTGCAAGATAGC

4688 SEQ ID N0:7480 0 -21.6 64.6 -20.9 0.1 -8.8 DEMANDE OU BREVET VOLUMINEUX
LA PRESENTE PARTIE DE CETTE DEMANDE OU CE BREVET COMPREND
PLUS D'UN TOME.

NOTE : Pour les tomes additionels, veuillez contacter le Bureau canadien des brevets JUMBO APPLICATIONS/PATENTS
THIS SECTION OF THE APPLICATION/PATENT CONTAINS MORE THAN ONE
VOLUME

NOTE: For additional volumes, please contact the Canadian Patent Office NOM DU FICHIER / FILE NAME
NOTE POUR LE TOME / VOLUME NOTE:

Claims (32)

WHAT IS CLAIMED IS:

1. An antisense compound targeted to a nucleic acid molecule encoding Nav1.3, wherein said antisense compound specifically hybridizes with and inhibits the expression of Nav1.3.

2. An antisense compound 8 to 30 nucleobases in length targeted to a nucleic acid molecule encoding Nav1.3, wherein said antisense compound specifically hybridizes with and inhibits the expression of Nav1.3.

The antisense compound of claim 2 wherein said antisense compound is an antisense oligonucleotide.

4. The antisense oligonucleotide of claim 3 comprising a nucleic acid sequence selected from the group consisting of at least eight contiguous bases of SEQ ID NO:1- SEQ ID NO:9094.

5. The antisense oligonucleotide of claim 3 comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:1 - SEQ ID NO:9094.

6. The antisense compound of claim 2, 3, 4, or 5 wherein the antisense oligonucleotide comprises at least one modified internucleoside linkage.

The antisense compound of claim 6 wherein the modified internucleoside linkage is a phosphorothioate linkage.

8. The antisense compound of claim 2, 3, 4, or 5 wherein the antisense oligomicleotide comprises at least one modified sugar moiety.

The antisense compound of claim 8 wherein the modified sugar moiety is a 2'-O-methoxyethyl sugar moiety.

10. The antisense compound of claim 2, 3, 4, or 5 wherein the antisense oligonucleotide comprises at least one modified nucleobase.

11. The antisense compound of claim 10 wherein the modified nucleobase is a 5-methylcytosine.

12. The antisense compound of claim 2, 3, 4, or 5 wherein the antisense oligonucleotide is a chimeric oligonucleotide.

13. A composition comprising the antisense compound of claim 2 and a pharmaceutically acceptable carrier or diluent.

14. The composition of claim 13 further comprising a colloidal dispersion system.

15. The composition of claim 13 wherein the antisense compound is an antisense oligonucleotide.

16. A method of inhibiting the expression of Nav1.3 in cells or tissues comprising contacting said cells or tissues with the antisense compound of claim 2 so that expression of Nav1.3 is inhibited.

17. Use of a therapeutically or prophylactically effective amount of the antisense compound of any one of claims 1 to 12 in the manufacture of a medicament for treating a human having a disease or condition associated with Nav1.3.

18. Use of a therapeutically or prophylactically effective amount of the antisense compound of any one of claims 1 to 12 for treating a human having a disease or condition associated with Nav1.3.

19. The use of claim 17 or claim 18 wherein the disease or condition is pain.

20. The use of claim 19 wherein the pain is selected from the group consisting of: neuropathic pain, post-herpetic neuralgia, chronic pain, lower back pain, diabetic neuropathy, trigeminal neuropathy, arthritic pain, acute pain, pain from burns, migraine headache, cluster headache, and mild-to-moderate headache.

21. The use of claim 17 or claim 18 wherein the disease or condition is a seizure disorder.

22. The use of claim 17 or claim 18 wherein the disease or condition is a childhood seizure disorder.

23. The use of claim 22 wherein childhood seizure disorder is neonatal or infantile epilepsy.

24. The use of claim 17 or claim 18 wherein the disease or condition is an ataxia.

25. The antisense compound of any one of claims 1 to 12 in a therapeutically or prophylactically effective amount for use in treating a disease or condition associated with Nav1.3.

26. The antisense compound of claim 25 wherein the disease or condition is pain.

27. The antisense compound of claim 26 wherein the pain is selected from the group consisting of: neuropathic pain, post-herpetic neuralgia, chronic pain, lower back pain, diabetic neuropathy, trigeminal neuropathy, arthritic pain, acute pain, pain from burns, migraine headache, cluster headache, and mild-to-moderate headache.

28. The antisense compound of claim 25 wherein the disease or condition is a seizure disorder.

29. The antisense compound of claim 25 wherein the disease or condition is a childhood seizure disorder.

30. The antisense compound of claim 29 wherein the childhood seizure disorder is neonatal or infantile epilepsy.

31. The antisense compound of claim 25 wherein the disease or condition is an ataxia.

32. A commercial package comprising a therapeutically or prophylactically effective amount of the antisense compound of any one of claims 1 to 12 together with instructions for use in treating a disease or condition associated with Nav1.3.