CA2245013A1 - Analytical measurement method and its use - Google Patents

Analytical measurement method and its use

Info

Publication number
CA2245013A1
CA2245013A1 CA002245013A CA2245013A CA2245013A1 CA 2245013 A1 CA2245013 A1 CA 2245013A1 CA 002245013 A CA002245013 A CA 002245013A CA 2245013 A CA2245013 A CA 2245013A CA 2245013 A1 CA2245013 A1 CA 2245013A1
Authority
CA
Canada
Prior art keywords
measurement
zones
hydrophilic
measurement zones
support
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002245013A
Other languages
French (fr)
Other versions
CA2245013C (en
Inventor
Heinz Eipel
Harald Keller
Burkhard Kroger
Sabine Philipp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Original Assignee
BASF SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF SE filed Critical BASF SE
Publication of CA2245013A1 publication Critical patent/CA2245013A1/en
Application granted granted Critical
Publication of CA2245013C publication Critical patent/CA2245013C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B01J2219/00619—Delimitation of the attachment areas by chemical means using hydrophilic or hydrophobic regions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00644—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being present in discrete locations, e.g. gel pads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed is an analytical measurement method for use in diagnosis, in research looking for active substances, in combinatorial chemistry, in crop protection, in toxicology or in environmental protection. This method makes a solid support which is essentially composed of an inert solid support material on which hydrophilic measurement zones which may be provided with a surface loading, are separated from one another by at least one hydrophobic coating in the form of separate zones around the hydrophilic measurement zones, where hte number of measurement points applied per cm2 of the support is greater than or equal to 10. In practise, the following steps are carried out:
a) application one or more times of mutually different reagents singly or in a mixture to the individual hydrophilic measurement zones on the support, b) treatment of the support with at least one reagent common to all the hydrophilic measurement zones so that the reagent is placed simultaneously on a plurality or all of the hydrophilic measurement zones, c) optionally washing all the measurement zones together after the application of the reagents or after the completion of the time for the reagents to react with one another, d) measurement, together or singly, of the measurement zones.
CA002245013A 1997-09-25 1998-09-24 Analytical measurement method and its use Expired - Fee Related CA2245013C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19742246A DE19742246A1 (en) 1997-09-25 1997-09-25 Analytical measurement method and its use
DE19742246.2 1997-09-25

Publications (2)

Publication Number Publication Date
CA2245013A1 true CA2245013A1 (en) 1999-03-25
CA2245013C CA2245013C (en) 2007-11-13

Family

ID=7843537

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002245013A Expired - Fee Related CA2245013C (en) 1997-09-25 1998-09-24 Analytical measurement method and its use

Country Status (5)

Country Link
EP (1) EP0905515B1 (en)
JP (1) JPH11160314A (en)
AT (1) ATE284536T1 (en)
CA (1) CA2245013C (en)
DE (2) DE19742246A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU779028B2 (en) * 1999-04-29 2005-01-06 Ciphergen Biosystems, Inc. Sample holder with hydrophobic coating for gas phase mass spectrometers
EP1053784A3 (en) * 1999-05-21 2001-05-23 Bruker Daltonik GmbH Processing samples in solutions having defined small contact area with support
DE19949735A1 (en) * 1999-10-15 2001-05-10 Bruker Daltonik Gmbh Processing of samples in solutions with a defined small wall contact area
WO2001034290A2 (en) 1999-11-09 2001-05-17 Sri International Array for generating combinatorial libraries
US7033840B1 (en) 1999-11-09 2006-04-25 Sri International Reaction calorimeter and differential scanning calorimeter for the high-throughput synthesis, screening and characterization of combinatorial libraries
SE0004352D0 (en) * 2000-11-27 2000-11-27 Helen Andersson System and method for connecting liquids in a microfluidic flow cell system
DE10120035B4 (en) * 2001-04-24 2005-07-07 Advalytix Ag Method and device for manipulating small quantities of liquid on surfaces
US7687256B2 (en) * 2002-04-11 2010-03-30 Spire Corporation Surface activated biochip
DE10246446B4 (en) * 2002-10-04 2006-05-24 Bruker Optik Gmbh Method for applying a sample film to a sample carrier
CN100514065C (en) * 2003-08-27 2009-07-15 松下电器产业株式会社 Microchip, process of manufacturing the same, and analytical method using the same
JP4505287B2 (en) * 2003-08-27 2010-07-21 パナソニック株式会社 Microchip, manufacturing method thereof, and inspection method using the same
JP4579752B2 (en) * 2005-04-07 2010-11-10 株式会社常光 Specimen spotting method using spotting support
EP2506973A2 (en) * 2009-12-02 2012-10-10 Roche Diagniostics GmbH Multiplexed microarray and method of fabricating thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5424219A (en) * 1991-10-25 1995-06-13 Cytech Biomedical, Inc. Method of performing assays for biomolecules and solid supports for use in such methods
US5846708A (en) * 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US6074609A (en) * 1996-04-24 2000-06-13 Glaxo Wellcome Inc. Systems for arraying beads
EP0912892B1 (en) * 1996-05-30 2008-09-17 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
JP2002514046A (en) * 1996-07-08 2002-05-14 バースタイン テクノロジーズ，インコーポレイティド Devices and methods for cleavable signaling factors
DE19628928A1 (en) * 1996-07-18 1998-01-22 Basf Ag Solid supports for analytical measurement processes, a process for their production and their use
JP4313861B2 (en) * 1997-08-01 2009-08-12 キヤノン株式会社 Manufacturing method of probe array

Also Published As

Publication number Publication date
ATE284536T1 (en) 2004-12-15
JPH11160314A (en) 1999-06-18
DE19742246A1 (en) 1999-04-01
EP0905515A3 (en) 2000-02-23
EP0905515A2 (en) 1999-03-31
DE59812354D1 (en) 2005-01-13
CA2245013C (en) 2007-11-13
EP0905515B1 (en) 2004-12-08

Similar Documents

Publication Publication Date Title
CA2245013A1 (en) Analytical measurement method and its use
NO990186L (en) Solid carriers for analytical measurement processes, their preparation methods and their use
HUP0100207A2 (en) Continuous format high throughput screening
AU6255299A (en) Formation of combinatorial arrays of materials using solution-based methodologies
ATE215401T1 (en) METHOD AND DEVICE FOR PRODUCING SAMPLE MATRIXES
CA2047637A1 (en) Analysis element and process for its manufacture
WO2000004382A8 (en) Arrays of proteins and methods of use thereof
NL194196B (en) Device and method for the stimulation of biological processes, which are related to the cellular activity.
CA2294040A1 (en) Retentate chromatography and protein chip arrays with applications in biology and medicine
WO2000058516A3 (en) Universal arrays
EP0657737A3 (en) Method and reagents for binding chemical analytes to a substrate surface, and related analytical devices and diagnostic techniques.
CA2139409A1 (en) Devices and methods for the measurement of cellular biochemical processes
EP0171072A3 (en) Method and device for the solid-phase sequencing of nucleic-acid fragments
CA2172363A1 (en) Bubble flushing syringe and method
DE19782096T1 (en) Immobilization of nucleic acids in high density
DE69011532D1 (en) BIOLOGICAL SUBSTRATE NERECTRIC ELECTRODE.
DK95284A (en) CRYSTALLIZED CARBOHYDRATE MATRIX FOR BIOLOGICALLY ACTIVE SUBSTANCES, PROCEDURES FOR THE PRODUCTION THEREOF AND ITS APPLICATION
DE60039519D1 (en) Immobilization of unmodified nucleic acids on acyl fluoride activated substrates
DE68906984D1 (en) APPARATUS FOR PERFORMING REPEATING CHEMICAL REACTIONS.
CA2276747A1 (en) Methods and devices for the reduction of small organic compounds from blood products
DE3877007D1 (en) METHOD FOR DRYING FINE DISTRIBUTED GOODS, ESPECIALLY GRAIN AND DEVICES FOR CARRYING OUT THIS METHOD.
CA2179567A1 (en) Element and System for Collecting, Transporting and Storing Sample Material to Be Analyzed
WO2002062821A3 (en) Peptide or protein microassay method and apparatus
IT1161590B (en) L-GLUTAMIC ACID OXIDASE (H202 GENERATOR), ITS PRODUCTION AND ANALYTICAL ASSAY USING IT
CA2220270A1 (en) Method for reducing inhibitors of nucleic acid hybridization

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed