CA2169282C - Method of improving the yield of herbicide-resistant crop plants - Google Patents
Method of improving the yield of herbicide-resistant crop plants Download PDFInfo
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- CA2169282C CA2169282C CA002169282A CA2169282A CA2169282C CA 2169282 C CA2169282 C CA 2169282C CA 002169282 A CA002169282 A CA 002169282A CA 2169282 A CA2169282 A CA 2169282A CA 2169282 C CA2169282 C CA 2169282C
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8277—Phosphinotricin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
- A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Method of improving the yield of crop plants which are resistant to glutamine synthetase inhibitors, in which plants are treated with glutamine synthetase inhibitors at low application rates, and to the use of glutamine synthetase inhibitors for improving the yield of transgenic crop plants.
Description
Description Method of improving the yield of herbicide-resistant crop plaata The compound glufoainate (glufoaiaats-aa~oaivm, ammonium DL-ho~moalania-4-yl(methyl)phosphiaate, Schwerdtle et al., Z. Pflanzenks. Pflanzenachutz., 1981, Special $ditioa IX, page 431) acts as a glutamiae syathetass (GS) inhibitor since it is a structural analog of glutamic acid. GS
playa a central role in the metabolism of all plaata. It is responsible for the detoxification of NH3 ia, sad, as a consequence, all terrestrial plants are damaged severely or destroyed after application of glufoainate since the asaimilatioa of a~aoaia is inhibited.
Plants which are resistant to the herbicidal activity of GS inhibitors were successfully produced by traaaferriag and exprea.aing a glufosinate acetyltransferaae geese isolated from frown straiaa of Streptcanycetea which produce bialaphos (phoaphiaothricia-alaayl-alaaiaa) (8B-B1-0 Z4Z 236 and EP-81-D Z57 542). Stands of such trane-a0 geaic, herbicide-tolerant crop plants can be kept weed-free is as efficient manner by post-emergence treatment with glucoaiaate.
Unexpectedly, field trials with such traaagnaic plants showed that the glufoaiaate-treated plaata give a measurably higher yield than untreated pleat staada. This higher yield is not a result of the excellent weed control by glufosinate sad its complete compatibility with the ataada of traaageaic crop plaate, but a positive effect of the herbicide treatment on growth sad yield.
The inveatioa therefore relates to a method of improving yield of crop plants which are resistant to glutamine synthetase inhibitors, which comprises treating the crop plants with at least one glutamine synthetase inhibitor at an application rate which is not harmful to the plants.
- Z -In particular, the invention relates to a method in which glutamiae aynthetaae inhibitors are employed for a yield-improviag treatment of pleats which are protected against the herbicidal activity of the glutamiae syathetase inhibitor by expression of an N-acetyltransferase gene.
The invention furthermore relatos to the use of a glut-amine synthetaae inhibitor for improving the yield of crop plants which are reeiataat to this inhibitor. In particular, it relates to the use of glutamiae ayathetase inhibitors for improving the yield of traasgenic crop plants.
The glutamine syathetase inhibitor used is preferably the comipound glufosinata or bialaphoa (Tachibana et al., Abatr. 5th Int. Coagr. Pestic. Chem., IVs, Abstract 19;
Mace, Jpn. Pestic. Inf., 1984, No. 45, p. Z7) . In this context, the-texm glufosinate embraces the racemats (DL-homoalanin-4-yl(methyl)phoephinic acid gas wall as the biologically active L isomor and the corresponding salts .
The herbicide can be employed in the commercially avail-able formulations. A further example of a GB inhibitor is the compound phosalacin (Omurs et al., J. of Antibiotics, Vol. 37, 8. pages 939-940, 1984).
The yield-improving effect of the treatment with glufoa-inate ie particularly pronounced whey the herbicidal treatment is carried out is the 2 to 8-, preferably the 3 to 6-leaf stage of the crop plants before flowering or, is the case of perennial plaata, at nay desired point in time.
In the method according to the invention, the pleats are treated at least once with the herbicide at application rates as they are also employed for weed control, for example 150 g - 1000 g of glufoeinate/ha.
However, the application rate required may vary as a function of the plants. their height sad the climatic conditions.
It is particularly advantageous to carry out the process using application rates of 350 - 700 g of glufosinate/ha.
Within this range of application rates, the affect achieved is proportional to the application rate of glufosinate, but not based on differences in the level of weed control. It is possible to achieve a weed control effect which is similar to the effect which can be achieved at higher application rates even when the application rate of PTC is low.
It is particularly advantageous to treat the plants repeatedly with low dosages in the lower range of the application concentrations, the treatment interval being a few days, i.e. between 2 and 30 days, preferably between 5 and 20, particularly preferably between 8 and 15 days. It is particularly advantageous to treat the plants with low dosages, the treatment interval being from 9 to 11 days.
The method according to the invention caa generally be used for the treatment of plants which are resistant to GS inhibitors. Resistant plants can also be obtained by conventional breeding methods. If the resistance level of plant obtained by conventional selection is similar to that of the transgenic plants, the plants obtained by conventional selection can also be treated by the method according to the invention. However, the method is particularly suitable for the treatment of glufosiaate-resistant plants which have been obtained by transferring a gene for resistance to the herbicide. EP-B1-0 242 236 and EP-H1-0 257 542 describe methods for producing such plants.
In this context, the term plants embraces crop plants from the group of the angiosperms and the gymnosperms.
The method according to the invention allows individual plants, but also crops of pleats, to be treated.
'~1 X9282 Particularly interesting among the gymnosperms is the class of the conifers.
Particularly interesting among the angiosperms are the plants from the families of the Solanaceae, Cruciferae, Compositae, Liliaceae, Vitacese, Chenopodiaceae, Ruta-ceae, Bromeliacese, Rubiaceae, Theaceae, Musaceae or Gramineae and the order of the Leguminosae. Represen-tatives of the families Solanaceae, Cruciferae and Graminese are preferably treated.
The method is of particular interest for the treatment of crop plants is which high yields are important, such as, for example, maize, soybeans. spring and winter oil seed rape, sugar beet, lucerne, sunflower, cotton, potatoes, wheat, barley and rice. However, it can also be used advantageously in tomatoes and other vegetables, such as cucumber, and fruits, such as melon, strawberries, raspberries, and kiwi fruit.
The use of the method in herbicide-resistant woody species is also particularly important, for example in plantations and nurseries.
Application of GS inhibitors, such as, for example, PTC
and its analogs and derivatives, to young specimens of woody species can accelerate the juvenile development. In this context, mention must be made, in particular, of walnut trees, oil palms, fruit trees, poplars and other cultivated plants which are woody species.
The method according to the invention ie therefore important both in agriculture and horticulture since application of the herbicidal glutamine synthetase inhibitor allows a clearly measurable increase in yield to be achieved without an additional application of fertilizer and plant growth regulators. The term increase in yield means in this context that the plant yield up to 50% more. Herbicides having different mechanisms of r, i '~,' (!
action either do not show such an effect or, frequently, have an adverse affect on yield.
The growth-enhancing activity of the glufosinate treat-ment can be measured is field trials and pot trials, by comparing yields of stands of plants which are treated with conventional herbicides or which were kept free from weeds by non-chemical methods.
The examples which follow are intended to illustrate the invention without thereby imposing any restriction.
IO Example 1 Transgenic glufosiaate-tolerant maize or soybean plants were planted in plots (10 m2) and, in the 3 - 5 leaf stage, treated with various amounts of glufosinate. The weed control level was scored 42 days after the applica-tioa. When the crops were ripe, the plots were harvested, and the seed yield was determined by weighing the kernels obtained.
When the maize plants were examined, Laddock~ (a mixture of atrazine and bentazone) wan employed as comparison product. Two products were employed for the treatment of the soya bean plants. Comparison product 1 contained a mixture of 134 g of fenoxaprop-P-ethyl/ha and 425 g of fomesafen/ha; comparison product 2 contained 2240 g of metolachlor/ha and 840 g of Storm~ (a mixture of benta-zone and acifluorfen)/ha. The comparison products are known from "The Pesticide Manual", 9th Edition, Hrit.
Crop Prot. Council, 1991.
The treatment described in Table 2, in which two low glufosinate dosage rates were used, was carried out at a 10 day interval.
f !_i ~ ) l w ;~
Table 1 Application Co~ariscai rates of Comparison product glufosinate (g of active substance/ha) 150 450 650 i i Weed control 92 97 98 78 t level in %
Yield in % of 118 121 125 100 the plot with the comparison product S . ._, I ~ L ~ L
U
'd O O N
N
L7~
a m ~i O
~
y ~ ~ ra Id O
a v m W
O
W
a O
' U
4l C1.
' b ~ d O 1l1 ~D N
, !
~ -1 N m ~ N + e y ~"~
U
b1 W tff ' O ~ .-i+ r-1 b m m ri N ~ O ~ N
m U
O
m ~ 0 U O O
~ ~
r1 tl1 ~ N
r~ u1 ri a m 0 o a~
M
Il1 O
~
O
a O
0 " ao ao W ~
~
ri a W i~O
O O m ra ~ -rl ~
N dP dP ed ..
a a a N ~r U rl ''i .pU U
~
b ~ y m b O
'~ a v 3 ~ ~ ..3
playa a central role in the metabolism of all plaata. It is responsible for the detoxification of NH3 ia, sad, as a consequence, all terrestrial plants are damaged severely or destroyed after application of glufoainate since the asaimilatioa of a~aoaia is inhibited.
Plants which are resistant to the herbicidal activity of GS inhibitors were successfully produced by traaaferriag and exprea.aing a glufosinate acetyltransferaae geese isolated from frown straiaa of Streptcanycetea which produce bialaphos (phoaphiaothricia-alaayl-alaaiaa) (8B-B1-0 Z4Z 236 and EP-81-D Z57 542). Stands of such trane-a0 geaic, herbicide-tolerant crop plants can be kept weed-free is as efficient manner by post-emergence treatment with glucoaiaate.
Unexpectedly, field trials with such traaagnaic plants showed that the glufoaiaate-treated plaata give a measurably higher yield than untreated pleat staada. This higher yield is not a result of the excellent weed control by glufosinate sad its complete compatibility with the ataada of traaageaic crop plaate, but a positive effect of the herbicide treatment on growth sad yield.
The inveatioa therefore relates to a method of improving yield of crop plants which are resistant to glutamine synthetase inhibitors, which comprises treating the crop plants with at least one glutamine synthetase inhibitor at an application rate which is not harmful to the plants.
- Z -In particular, the invention relates to a method in which glutamiae aynthetaae inhibitors are employed for a yield-improviag treatment of pleats which are protected against the herbicidal activity of the glutamiae syathetase inhibitor by expression of an N-acetyltransferase gene.
The invention furthermore relatos to the use of a glut-amine synthetaae inhibitor for improving the yield of crop plants which are reeiataat to this inhibitor. In particular, it relates to the use of glutamiae ayathetase inhibitors for improving the yield of traasgenic crop plants.
The glutamine syathetase inhibitor used is preferably the comipound glufosinata or bialaphoa (Tachibana et al., Abatr. 5th Int. Coagr. Pestic. Chem., IVs, Abstract 19;
Mace, Jpn. Pestic. Inf., 1984, No. 45, p. Z7) . In this context, the-texm glufosinate embraces the racemats (DL-homoalanin-4-yl(methyl)phoephinic acid gas wall as the biologically active L isomor and the corresponding salts .
The herbicide can be employed in the commercially avail-able formulations. A further example of a GB inhibitor is the compound phosalacin (Omurs et al., J. of Antibiotics, Vol. 37, 8. pages 939-940, 1984).
The yield-improving effect of the treatment with glufoa-inate ie particularly pronounced whey the herbicidal treatment is carried out is the 2 to 8-, preferably the 3 to 6-leaf stage of the crop plants before flowering or, is the case of perennial plaata, at nay desired point in time.
In the method according to the invention, the pleats are treated at least once with the herbicide at application rates as they are also employed for weed control, for example 150 g - 1000 g of glufoeinate/ha.
However, the application rate required may vary as a function of the plants. their height sad the climatic conditions.
It is particularly advantageous to carry out the process using application rates of 350 - 700 g of glufosinate/ha.
Within this range of application rates, the affect achieved is proportional to the application rate of glufosinate, but not based on differences in the level of weed control. It is possible to achieve a weed control effect which is similar to the effect which can be achieved at higher application rates even when the application rate of PTC is low.
It is particularly advantageous to treat the plants repeatedly with low dosages in the lower range of the application concentrations, the treatment interval being a few days, i.e. between 2 and 30 days, preferably between 5 and 20, particularly preferably between 8 and 15 days. It is particularly advantageous to treat the plants with low dosages, the treatment interval being from 9 to 11 days.
The method according to the invention caa generally be used for the treatment of plants which are resistant to GS inhibitors. Resistant plants can also be obtained by conventional breeding methods. If the resistance level of plant obtained by conventional selection is similar to that of the transgenic plants, the plants obtained by conventional selection can also be treated by the method according to the invention. However, the method is particularly suitable for the treatment of glufosiaate-resistant plants which have been obtained by transferring a gene for resistance to the herbicide. EP-B1-0 242 236 and EP-H1-0 257 542 describe methods for producing such plants.
In this context, the term plants embraces crop plants from the group of the angiosperms and the gymnosperms.
The method according to the invention allows individual plants, but also crops of pleats, to be treated.
'~1 X9282 Particularly interesting among the gymnosperms is the class of the conifers.
Particularly interesting among the angiosperms are the plants from the families of the Solanaceae, Cruciferae, Compositae, Liliaceae, Vitacese, Chenopodiaceae, Ruta-ceae, Bromeliacese, Rubiaceae, Theaceae, Musaceae or Gramineae and the order of the Leguminosae. Represen-tatives of the families Solanaceae, Cruciferae and Graminese are preferably treated.
The method is of particular interest for the treatment of crop plants is which high yields are important, such as, for example, maize, soybeans. spring and winter oil seed rape, sugar beet, lucerne, sunflower, cotton, potatoes, wheat, barley and rice. However, it can also be used advantageously in tomatoes and other vegetables, such as cucumber, and fruits, such as melon, strawberries, raspberries, and kiwi fruit.
The use of the method in herbicide-resistant woody species is also particularly important, for example in plantations and nurseries.
Application of GS inhibitors, such as, for example, PTC
and its analogs and derivatives, to young specimens of woody species can accelerate the juvenile development. In this context, mention must be made, in particular, of walnut trees, oil palms, fruit trees, poplars and other cultivated plants which are woody species.
The method according to the invention ie therefore important both in agriculture and horticulture since application of the herbicidal glutamine synthetase inhibitor allows a clearly measurable increase in yield to be achieved without an additional application of fertilizer and plant growth regulators. The term increase in yield means in this context that the plant yield up to 50% more. Herbicides having different mechanisms of r, i '~,' (!
action either do not show such an effect or, frequently, have an adverse affect on yield.
The growth-enhancing activity of the glufosinate treat-ment can be measured is field trials and pot trials, by comparing yields of stands of plants which are treated with conventional herbicides or which were kept free from weeds by non-chemical methods.
The examples which follow are intended to illustrate the invention without thereby imposing any restriction.
IO Example 1 Transgenic glufosiaate-tolerant maize or soybean plants were planted in plots (10 m2) and, in the 3 - 5 leaf stage, treated with various amounts of glufosinate. The weed control level was scored 42 days after the applica-tioa. When the crops were ripe, the plots were harvested, and the seed yield was determined by weighing the kernels obtained.
When the maize plants were examined, Laddock~ (a mixture of atrazine and bentazone) wan employed as comparison product. Two products were employed for the treatment of the soya bean plants. Comparison product 1 contained a mixture of 134 g of fenoxaprop-P-ethyl/ha and 425 g of fomesafen/ha; comparison product 2 contained 2240 g of metolachlor/ha and 840 g of Storm~ (a mixture of benta-zone and acifluorfen)/ha. The comparison products are known from "The Pesticide Manual", 9th Edition, Hrit.
Crop Prot. Council, 1991.
The treatment described in Table 2, in which two low glufosinate dosage rates were used, was carried out at a 10 day interval.
f !_i ~ ) l w ;~
Table 1 Application Co~ariscai rates of Comparison product glufosinate (g of active substance/ha) 150 450 650 i i Weed control 92 97 98 78 t level in %
Yield in % of 118 121 125 100 the plot with the comparison product S . ._, I ~ L ~ L
U
'd O O N
N
L7~
a m ~i O
~
y ~ ~ ra Id O
a v m W
O
W
a O
' U
4l C1.
' b ~ d O 1l1 ~D N
, !
~ -1 N m ~ N + e y ~"~
U
b1 W tff ' O ~ .-i+ r-1 b m m ri N ~ O ~ N
m U
O
m ~ 0 U O O
~ ~
r1 tl1 ~ N
r~ u1 ri a m 0 o a~
M
Il1 O
~
O
a O
0 " ao ao W ~
~
ri a W i~O
O O m ra ~ -rl ~
N dP dP ed ..
a a a N ~r U rl ''i .pU U
~
b ~ y m b O
'~ a v 3 ~ ~ ..3
Claims (6)
1. A method of improving yield of crop plants which are resistant to glutamine synthetase inhibitors, which comprises treating the crop plants with at least one glutamine synthetase inhibitor at an application rate which is not harmful to the plants.
2. The method as claimed in claim 1, wherein glufosinate and salts thereof are employed for the yield-improving treatment of crop plants which are protected against the herbicidal property of the glutamine synthetase inhibitor by expression of an n-acetyl-transferase gene.
3. The method as claimed in claim 1 or claim 2, wherein the crop plants are treated at least once with the active substance at an application rate as also employed for weed control.
4. The method as claimed in any one of claims 1 to 3, wherein the crop plants are treated at least once using 150-1000 g of glufosinate/ha.
5. The method as claimed in any one of claims 1 to 3, wherein the crop plants are treated at least once using 350-700 g of glufosinate/ha.
6. Use of a glutamine synthetase inhibitor for improving yield of crop plants which are resistant to this inhibitor.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE4327056A DE4327056A1 (en) | 1993-08-12 | 1993-08-12 | Process for increasing the yield of herbicide-resistant crops |
| DEP4327056.5 | 1993-08-12 | ||
| PCT/EP1994/002598 WO1995005082A1 (en) | 1993-08-12 | 1994-08-05 | Method of increasing the yield of herbicide-resistant crop plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2169282A1 CA2169282A1 (en) | 1995-02-23 |
| CA2169282C true CA2169282C (en) | 2005-03-29 |
Family
ID=6495008
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002169282A Expired - Lifetime CA2169282C (en) | 1993-08-12 | 1994-08-05 | Method of improving the yield of herbicide-resistant crop plants |
Country Status (19)
| Country | Link |
|---|---|
| EP (1) | EP0714237B2 (en) |
| JP (1) | JPH09501179A (en) |
| KR (1) | KR100311838B1 (en) |
| CN (1) | CN1089555C (en) |
| AT (1) | ATE172847T1 (en) |
| AU (1) | AU700325B2 (en) |
| BR (1) | BR9407237A (en) |
| CA (1) | CA2169282C (en) |
| CZ (1) | CZ286623B6 (en) |
| DE (2) | DE4327056A1 (en) |
| DK (1) | DK0714237T4 (en) |
| ES (1) | ES2124906T5 (en) |
| HU (1) | HU220775B1 (en) |
| NZ (1) | NZ271372A (en) |
| PL (1) | PL180005B1 (en) |
| RU (1) | RU2166850C2 (en) |
| UA (1) | UA37243C2 (en) |
| WO (1) | WO1995005082A1 (en) |
| ZA (1) | ZA946038B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5633434A (en) * | 1990-02-02 | 1997-05-27 | Hoechst Aktiengesellschaft | Transgenic plants displaying virus and phosphinothricin resistance |
| US5739082A (en) * | 1990-02-02 | 1998-04-14 | Hoechst Schering Agrevo Gmbh | Method of improving the yield of herbicide-resistant crop plants |
| ATE219329T1 (en) * | 1996-03-29 | 2002-07-15 | Monsanto Europe Sa | NEW USE OF N-(PHOSPHONOMETHYL)-GLYCINE AND DERIVATIVES THEREOF |
| DE19836659A1 (en) * | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Use of synergistic herbicide combination including glufosinate- or glyphosate-type, imidazolinone, protoporphyrinogen oxidase inhibitory azole or hydroxybenzonitrile herbicide, to control weeds in cotton |
| DE19836684A1 (en) * | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Use of a synergistic herbicidal combination including a glufosinate- or glyphosate-type, imidazolinone or protoporphyrinogen oxidase to control weeds in rice |
| DE19836673A1 (en) * | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Use of a synergistic herbicidal combination including a glufosinate- or glyphosate-type or imidazolinone herbicide to control weeds in sugar beet |
| DE19836726A1 (en) * | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Use of a synergistic herbicide combination including glufosinate- or glyphosate-type, imidazolinone or protoporphyrinogen oxidase inhibitory azole herbicide to control weeds in oil seed rape |
| PL217233B1 (en) * | 1998-08-13 | 2014-06-30 | Bayer Cropscience Ag | Application of herbicide composition, method for controlling weeds and herbicide formulation |
| DE19836700A1 (en) * | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Use of a synergistic herbicide combination including a glufosinate- or glyphosate-type, imidazolinone or protoporphyrinogen oxidase inhibitory azole herbicide to control weeds in cereals |
| DE19836660A1 (en) * | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Use of a synergistic herbicide combination including a glufosinate- or glyphosate-type, imidazolinone or protoporphyrinogen oxidase inhibitory azole herbicide to control weeds in soya |
Family Cites Families (3)
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|---|---|---|---|---|
| DE3200486A1 (en) * | 1982-01-09 | 1983-07-21 | Hoechst Ag, 6230 Frankfurt | Use of (3-amino-3-carboxy)alkylmenthylphosphinic acid and derivatives thereof for increasing the yield in plants |
| DE3765449D1 (en) † | 1986-03-11 | 1990-11-15 | Plant Genetic Systems Nv | PLANT CELLS RESISTED BY GENE TECHNOLOGY AND RESISTANT TO GLUTAMINE SYNTHETASE INHIBITORS. |
| EP0481407A1 (en) * | 1990-10-18 | 1992-04-22 | Hoechst Aktiengesellschaft | Process for enhancing the formation of carbohydrates in plants |
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1993
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1994
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- 1994-08-05 DK DK94924848T patent/DK0714237T4/en active
- 1994-08-05 CA CA002169282A patent/CA2169282C/en not_active Expired - Lifetime
- 1994-08-05 AU AU74979/94A patent/AU700325B2/en not_active Expired
- 1994-08-05 CN CN94193033A patent/CN1089555C/en not_active Expired - Lifetime
- 1994-08-05 HU HU9600295A patent/HU220775B1/en unknown
- 1994-08-05 BR BR9407237A patent/BR9407237A/en not_active IP Right Cessation
- 1994-08-05 KR KR1019960700694A patent/KR100311838B1/en not_active IP Right Cessation
- 1994-08-05 PL PL94312982A patent/PL180005B1/en unknown
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- 1994-08-05 ES ES94924848T patent/ES2124906T5/en not_active Expired - Lifetime
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- 1994-08-05 AT AT94924848T patent/ATE172847T1/en active
- 1994-08-05 EP EP94924848A patent/EP0714237B2/en not_active Expired - Lifetime
- 1994-08-05 RU RU96107216/04A patent/RU2166850C2/en active
- 1994-08-11 ZA ZA946038A patent/ZA946038B/en unknown
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| PL312982A1 (en) | 1996-05-27 |
| ES2124906T5 (en) | 2004-06-01 |
| CA2169282A1 (en) | 1995-02-23 |
| WO1995005082A1 (en) | 1995-02-23 |
| HUT74593A (en) | 1997-01-28 |
| HU220775B1 (en) | 2002-05-28 |
| BR9407237A (en) | 1996-09-24 |
| AU700325B2 (en) | 1998-12-24 |
| EP0714237B1 (en) | 1998-11-04 |
| CZ286623B6 (en) | 2000-05-17 |
| DE59407241D1 (en) | 1998-12-10 |
| HU9600295D0 (en) | 1996-04-29 |
| EP0714237B2 (en) | 2003-09-17 |
| ZA946038B (en) | 1995-03-20 |
| DK0714237T3 (en) | 1999-07-19 |
| ES2124906T3 (en) | 1999-02-16 |
| KR960703516A (en) | 1996-08-31 |
| AU7497994A (en) | 1995-03-14 |
| ATE172847T1 (en) | 1998-11-15 |
| CZ41296A3 (en) | 1996-05-15 |
| KR100311838B1 (en) | 2002-11-22 |
| DE4327056A1 (en) | 1995-02-16 |
| CN1089555C (en) | 2002-08-28 |
| EP0714237A1 (en) | 1996-06-05 |
| UA37243C2 (en) | 2001-05-15 |
| RU2166850C2 (en) | 2001-05-20 |
| JPH09501179A (en) | 1997-02-04 |
| CN1128938A (en) | 1996-08-14 |
| NZ271372A (en) | 1997-10-24 |
| PL180005B1 (en) | 2000-11-30 |
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