AU2015221459A1 - Peptides targeting tnf family receptors and antagonizing tnf action, compositions, methods and uses thereof - Google Patents

Abstract

The present invention provides modulators of TNF, particularly peptides and their derivatives, particularly GEP peptides, which antagonize TNF and TNF-mediated responses, activity or signaling. The invention provides methods of antagonizing TNF and the modulation of TNF mediated diseases or responses, including inflammatory diseases and conditions. Compositions of GEP peptides, including in combination with other inflammatory mediators, are provided. Methods of treatment, alleviation, or prevention of TNF-mediated diseases and inflammatory conditions, including rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, psoriasis, inflammatory bowel diseases, Chrohn's disease, ulcerative colitis, uveitis, inflammatory lung diseases, chronic obstructive pulmonary disease, are provided.

Description

PEPTIDES TARGETING TNF FAMILY RECEPTORS AND ANTAGONIZING TNF ACTION, COMPOSITIONS, METHODS AND USES THEREOF GOVERNMENTAL SUPPORT 10011 This invention was made with government support under NIH/NIAMS 1 KOI AR053210 and NIH/NIA 1 R03 AG029388, awarded by the National Institute of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases. The government has certain rights in the invention. FIELD OF THE INVENTION [0021 The present invention relates generally to modulators of TNF/TNFR, particularly peptides and their derivatives which antagonize TNF and TNF/TNFR-mediated responses, activity or signaling. The invention also relates to methods of antagonizing TNF and the modulation of TNF-mediated diseases or responses, including inflammatory diseases and conditions. BACKGROUND OF THE INVENTION [003] In the progress of arthritis, synovium, cartilage, and bone are all sites of increased production of growth factors, cytokines, and inflammatory mediators that are believed to contribute to pathogenesis [1, 2]. Although both bone and synovium have important roles in the pathogenesis of arthritis [1, 31, most efforts at developing disease-modifying treatments have focused on the molecular events within cartilage. Arthritic chondrocytes undergo a series of complex changes, including proliferation, catabolic alteration, and, ultimately, death. The regulation of these phenotypic changes at different stages of disease is under intensive study, with focus on the biomechanical and biochemical signals that regulate each of these discrete chondrocyte responses [2, 4]. Chondrocytes themselves are major protagonists in this regulatory cascade-notjust the target of external biomechanical and biochemical stimuli, but themselves the source of cytokines, proteases, and inflammatory mediators that promote the deterioration of articular cartilage [1, 2]. Pathogenic molecules produced by arthritic chondrocytes include tumor necrosis factor (TNF), interleukin-1 (IL-I), IL-6, IL-8, matrix metalloproteinases (MMPs), ADAMTSs,nitric oxide, prostaglandins, and leukotrienes [2, 4]. There is also evidence that arthritic chondrocytes exhibit increased anabolic activity, including increased release of growth factors and synthesis of type II collagen, proteoglycan, and other extracellular matrix proteins, as well as the expression of genes associated with the chondroprogenitor hypertrophic phenotype [5-7]. [004] A great deal of research in rheumatology over the past two decades has focused on identifying cytokines and mediators responsible for the inflammatory and degenerative processes in rheumatoid arthritis (RA), with the aim of developing specific antagonists of therapeutic value. Among all factors, TNF-a has received the greatest attention because of its position at the apex of the pro-inflammatory cytokine cascade, and its dominance in the pathogenesis of RA. Many lines of evidence support this theory including: (1) TNF-a is expressed at high levels in inflamed synovium and cartilage from RA patients; (2) anti-TNF-a inhibits the production of other pro-inflammatory cytokines including IL-I; and (3) TNF-a can inducejoint inflammation, trigger cartilage destruction by inducing metalloproteinase, and stimulate osteoclastogenesis and bone resorption. Most importantly, anti-TNF therapies for RA have shown remarkable results by decreasing inflammation, improving patient function and vitality, and attenuating cartilage and bone erosions. There are now three anti-TNF treatments via targeting to TNF ligand, etanercept (Enbrel, a soluble TNFR2-lgGl fusion protein), infliximab (Remicade, a chimeric monoclonal antibody against TNF-a), and adalimumab (a humaneric monoclonal antibody against TNF-a) that have been used clinically for treating various kinds of inflammatory diseases, including rheumatoid arthritis. Engineered proteins/peptides are now providing a new wave of therapeutic products. Indeed, designed protein/peptide therapeutic agents now outnumber and surpass the number of new small-molecule drugs approved annually by the FDA. Antibodies and immunoadhesins that directly target cytokines for their systemic removal (ligand ablation) have become an effective therapeutic strategy (e.g. etanercept, adalimumab and infliximab), and in some indications the selective targeting of cytokine receptors (e.g. anakinra) can deliver a highly effective clinical outcome. [005] Granulin/epithelin precursor (GEP), also known as PC-cell-derived growth factor (PCDGF), acrogranin, progranulin (PGRN), proepithelin (PEPI), or GP80, was first purified as a growth factor from conditioned tissue culture media [8, 9, 65, 66, 67]. GEP is a 593-amino-acid secreted glycoprotein with an apparent molecular weight of 80 kDa [10, 14], which acts as an autocrine growth factor. GEP contains seven and a half repeats of a cysteine-rich InOtJf(CX 5

CX

5

CCX

5

CCX

6

CCXDX

2

HCCPX

4

CX

5

.

6 C) (SEQ ID NO: 9) in the order P-G-F-B-A-C-D-E, where A-G are full repeats and P is the half motif (FIGURE 2 i). The C-terminal region of the consensus sequence contains the conserved sequence CCXDXHCCP (SEQ ID NO: 10) and is suggested to have a metal binding site and to be involved in regulatory function [15]. Notably, GEP undergoes proteolytic processing with the liberation of small, 6-kDa repeat units known as granulins (or epithelins), which retain biological activity [16]: peptides are active in cell growth assays [13] and may be related to inflammation[17]. [006] GEP is abundantly expressed in rapidly cycling epithelial cells, in cells of the immune system, and in neurons [10-12, 17]. High levels of GEP expression are also found in several human cancers and contribute to tumorigenesis in diverse cancers, including breast cancer, clear cell renal carcinoma, invasive ovarian carcinoma, glioblastoma, adipocytic teratoma, and multiple mycloma [16, 18-24]. Although GEP mainly functions as a secreted growth factor, it was also found to be localized inside cells and to directly modulate intracellular activities [12, 25-27]. The role of GEP in the regulation of cellular proliferation has been well characterized using mouse embryo fibroblasts derived from mice with a targeted deletion of the insulin-like growth factor receptor (IGF-IR) gene (R- cells). These cells are unable to proliferate in response to IGF-l and other growth factors (EGF and PDGF) necessary for progression through the cell cycle [28]. In contrast, GEP is the only known growth factor able to bypass the requirement for the IGF-IR, thus promoting cell growth of R- cells [13, 29]. Increasing evidence has also implicated GEP in the regulation of differentiation, development and pathological processes. It has been isolated as a differentially-expressed gene from mesothelial differentiation [30], sexual differentiation of the brain [31], macrophage development [32], and synovium of rheumatoid arthritis and osteoarthritis [33]. GEP was also shown to be a crucial mediator of wound response and tissue repair [21, 34]. It was reported that mutations in GEP cause tau-negative frontotemporal dementia linked to chromosome 17 [35-38]. The mode of action of GEP remains largely unknown. Several GEP-associated proteins have been reported to affect GEP action in various processes. One example is the secretory leukocyte protease inhibitor (SLPI). Elastase digests GEP exclusively in the intergranulin linkers, resulting in the generation of granulin peptides, suggesting that this protease may be an important GEP convertase. SLPI blocks this proteolysis either by directly binding to elastase or by sequestering granulin peptides from the enzyme [34]. GEP can modulate transcriptional activities by interacting with human cyclin TI [26] and Tat-P-TEFb [25]. GEP was also found to interact with perlecan, a heparan sulfate proteoglycan; perlecan-null mice exhibit severe skeletal defects [19, 39-41]. [0071 The Tumor Necrosis Factor (TNF) family of cytokines plays an essential role in multiple biological functions including inflammation, organogenesis, host defense, autoimmunity, and apoptosis. 3 The action of these potent biological mediators is achieved through a receptor-ligand interaction, leading to intracellular signaling and a change in cellular phenotype. The ligands exert their function by forming trimers and binding to their corresponding receptors. Subsequent receptor oligomerization results in conformational change of the receptor's intracellular domain, which then allows for members of the TNF receptor-associated factor (TRAF) family of adaptor proteins to bind and initiate a signaling cascade. TNFR2, TNFRl, TrkA, NGFR, CD 40, CD 30, OX-40, DR5, DR3, DR4 and RANK include some of the members of TNF receptor super-family that interact with different TRAF molecules (including 1-6) (Lewit-Bentley, A., et al., J. MoL Biol. 199:389-392(1988), Banner, D. W., et al., Cel. 73:431-445(1993), Karpusas, M., et al., Structure. 3:1031-1039(1995), Hymowitz, S. G., et al., Mol. Cell. 4:563-571(1999), Mongkilsapaya, J., et al., Nat. Strue. Biol. 6:- 1048- 1053(1 999), Cha, S. S., et a., J. Biol. Chem. 275:31 171-31 177(2000)). [0081 Both TNF receptors (TNFR and TNFR2) are ubiquitously expressed in cells and interact with their cognate ligand: TNFa, a central proinflammatory cytokine [42-44]. It is widely accepted that TNF a serves very important functions in pathophysiology, being a factor that interferes strongly with the cell growth, differentiation and death. TNF appears not only to orchestrate acute responses to infection and immunological injury but also to act as a balancing factor required for the re-establishment of physiological homeostasis and regulation [45]. TNFx has been found to affect skeletal development: its level is increased in most inflammatory diseases known to affect longitudinal growth in children [46, 47] and catch-up growth was shown in children with refractory juvenile idiopathic arthritis treated with the TNF antagonist etanercept (Enbrel) [46, 47]; TNFoaregulates growth plate chondrocytes and suppress longitudinal growth in metatarsal organ cultures [48]. [0091 Arthritis is a degenerative joint disease, occurring primarily in the senior population, that currently affects more than 46,000,000 individuals in the United States. Typical clinical symptoms are pain and stiffness, particularly after prolonged activity. In industrialized societies arthritis is the leading cause of physical disability, increased health care usage, and impaired quality of life. The impact of arthritic conditions is expected to grow as the population both increases and ages in the coming decades. Despite the prevalence of arthritic diseases, their precise etiology, pathogenesis, and progression remain beyond our understanding. Evidence is accumulating that demonstrates the significance of inflammatory cytokines and growth factors in the pathological processes of arthritis. The destruction of the extracellular matrices of articular cartilage and bone in arthritic joints is thought to be mediated by excessive cytokine activities and imbalance between inflammatory cytokines and their physiological antagonists. The 4 isolation of the growth factors that regulates chondrocytes and arthritis, and the inhibitors that antagonize the actions of cytokines, are therefore of great importance from both a pathophysiological and a therapeutic standpoint. We have previously identified granulin/epithelin precursor (GEP) as a novel chondrogenic growth factor that plays an essential role in cartilage formation (Xu, K et a] (2007) J Biol Chem 282(15):l 1347-1 1355; WO 2008/094687 A2). [0010] There still exists a need in the art for a better and more complete understanding of the process of and, thereby, intervention for, inflammatory diseases and conditions, particularly TNF family member mediated processes and conditions. Thus, the purpose of this invention is to extend our understanding of the molecular mechanisms by which growth factors and cytokines control cartilage development and arthritis, and to mediators thereof for development of new anti-TNF/TNFR therapeutic interventions for various kinds of TNF-related diseases, including inflammatory arthritis. [0011] The citation of references herein shall not be construed as an admission that such is prior art to the present invention. SUMMARY QF THE INVENTION [0012] Taking into account the biological properties of GEP, it has been hypothesized that GEP could act through "classic" membrane receptors), as do other known growth factors. Thus far, a functional receptor has not been identified. Our functional genetic screen described herein led to the isolation of TNF receptors as novel GEP-binding receptors. Our studies demonstrate that GEP (Granulin/epithelin precursor) is the first growth factor that directly targets to TNF receptors (TNFR). Thus GEP and its derived peptide(s) represent a novel anti-TNF/TNFR signaling blocker by acting on the cytokine receptors. [0013] The studies described herein demonstrate that GEP is a novel antagonist of TNF/TNFR signaling. The present findings reveal that: 1) GEP directly binds to TNF receptors in a dose-dependent manner; 2) Blocking TNF receptors by neutralizing antibodies or recombinant extracellular domains abolishes GEP function in stimulation of cell proliferation; 3) GEP potently activates Erkl/2 signaling, and moderately Akt pathway; 4) GEP activates genes known to be the downstream molecules of the TGF P subfamily, including BMP2; in addition, GEP-mediated inductions of these gene expressions depend on TNF 5 receptors; 5) GEP is an arthritis-responsive gene and its level was significantly elevated in patients with arthritis; 6) GEP, as an antagonist of TNFa, dramatically reduces inflammation response and apoptosis induced by TNFa; and 7) importantly, GEP exhibits better (at least as good as) inhibition of TNF-stimulated inflammation than Enbrel and Remicade that have been used clinically for treating various kinds of inflammatory diseases and conditions, including rheumatoid arthritis. These findings reveal that GEP is a novel naturally-occurring antagonist of TNF/TNFR signaling via directly targeting to TNF receptors. [0014] The invention provides GEP and GEP peptides, particularly including the peptide(s) denoted atsttrin, as modulators of TNF/TNFR activity and signaling, particularly inhibiting or blocking TNF-mediated signaling or response, particularly as antagonists of TNF/TNFR. [0015] The invention provides peptides which antagonize TNF family member receptors, particularly TNFR, and block, inhibit, reduce, or prevent TNF family member signaling, including TNF/TNFR signaling. The invention provides peptides which antagonize TNF family member receptors, particularly RANK, and block, inhibit, reduce, or prevent TNF family member signaling, including RANK/RANKL signaling. [0016] In a particular embodiment, the present invention relates to all members of the herein disclosed family of GEP peptides and of atsttrin, which are capable of modulating, particularly antagonizing, TNF family/receptor signaling and response, particularly TNF/TNFR signaling and response. The family of peptides includes fragments or portions, including mixed portions of GEP sequence and half units, particularly comprising one or more granulin unit and one or more linker unit of GEP. In one aspect the peptide comprises two or more half units of granulin units and one or more linker unit of GEP. [0017] In a particular aspect of the invention, the GEP peptide comprises the peptide atsttrin, comprising combinations of half units of granulin units A, C and F in combination with linker units P3, P4 and P5. In a particular aspect, the GEP peptide comprises a combination of half units of granulin units, wherein at least one half unit is /z F, and linker units, particularly at least two linker units. In a further particular aspect atsttrin has the amino acid sequence set out in FIGURE 24 and comprises granulin units and linker units WF-P3-P4-/aA-P5-% C, including as set out in SEQ ID NO: 2. 6 [0018] The present invention naturally contemplates several means for preparation of the GEP peptides and/or atsttrin of the present invention, including as illustrated herein and/or using known recombinant techniques, and the invention is accordingly intended to cover such synthetic preparations within its scope. The determination of the antagonist amino acid sequences disclosed herein facilitates the reproduction of the peptides by any of various synthetic methods or any known recombinant techniques, and accordingly, the invention extends to expression vectors comprising nucleic acid encoding the peptides of the present invention for expression in host systems by recombinant DNA techniques, and to the resulting transformed hosts, [00191 The present invention also relates to a recombinant DNA molecule, recombinant nucleic acid, or cloned gene, or a degenerate variant thereof, preferably a nucleic acid molecule, in particular a recombinant DNA molecule or cloned gene, encoding the amino acid of one or more GEP peptides shown in FIGURE 24 or variants thereof, In a particular embodiment, the recombinant DNA molecule, recombinant nucleic acid, or a degenerate variant thereof, preferably a nucleic acid molecule, encodes a GEP peptide capable of antagonizing TNF/TNFR, which comprises one or more granulin unit and one or more linker unit of GEP as depicted in FIGURE l and as set out in the sequence of GEP of FIGURE 23. hi a further particular embodiment, the recombinant DNA molecule, recombinant nucleic acid, or a degenerate variant thereof, preferably a nucleic acid molecule, encodes a GEP peptide atsttrin capable of antagonizing TNF/TNFR as set out in FIGURE 24 and comprising granulin units and linker units %F-P3 P4-'/2A-P5-!/C (SEQ ID NO: 2). [0020] It is an object of the present invention to provide pharmaceutical compositions for use in therapeutic methods which comprise or are based upon the GEP peptides and/or atsttrin. The pharmaceutical compositions include combinations of one or more GEP peptides and/or atsttrin having TNF antagonistic activity. The pharmaceutical compositions include combinations of one or more GEP peptides and/or atsttrin having TNF antagonistic activity and one or more inflammatory mediator. Inflammatory mediators include and may be selected from non-steroidal anti-inflammatory agents (NSAIDs), steroids, corticosteroids, other TNF antagonists (e.g. etanercept, adalimumab and infliximab), and cytokine receptor antagonists (e.g. anakinra). The pharmaceutical compositions include combinations of one or more GEP peptides and/or atsttrin having TNF antagonistic activity and one or more inflammatory mediator, immunododulatory agent, or anti-cancer agent. 7 [0021] In a further embodiment, the present invention relates to certain therapeutic methods which would be based upon the TNF/TNFR antagonistic activity of GEP, GEP peptides and/or atsttrin, or active fragments thereof, or upon agents or other drugs determined to possess the same activity. Thus, the present invention provides methods of preventing and/or treating diseases mediated by TNF/TNFR activity and/or which are facilitated or induced by TNF family ligand/receptor activity and/or characterized by inflammation. The invention provides methods of treatment, alleviation, or prevention of TNF-mediated diseases and inflammatory conditions or immunological conditions, including rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, psoriasis, inflammatory bowel diseases, Chrohn's disease, ulcerative colitis, uveitis, inflammatory lung diseases, chronic obstructive pulmonary disease. [0022] In one aspect, the invention provides a method for treatment, alleviation or prevention of tumors or cancer which are mediated by GEP, TNF, GEP/TNFR and/or TNF/TNFR. Thus, it is contemplated that a tumor, cancerous or precancerous condition wherein the growth and/or proliferation of the tumor or cancerous/precancerous cells are dependent on or facilitated by GEP, TNF, GEP/TNFR and/or TNF/TNFR activity or signaling may be sensitive to the TNF/GEP antagonist peptide(s) of the present invention. The invention further contemplates use and application of GEP peptides, particularly including atsttrin to inhibit or block GEP-mediated cancer or cell proliferation. [0023] More specifically, the therapeutic method provides for methods for the treatment, prevention or alleviation of diseases mediated by TNF/TNFR activity and/or which are facilitated or induced by TNF family ligand/receptor activity and/or characterized by inflammation by the administration of pharmaceutical compositions that comprise effective antagonists of TNF based on GEP, GEP peptides and/or atsttrin or its subunits, or other equally effective drugs developed for instance by a drug screening assay prepared and used in accordance with a further aspect of the present invention. In a particular aspect, GEP, GEP peptides, and/or atsttrin may be administered to treat, alleviate, or prevent a TNF/TNFR mediated disease or condition or an inflammatory disease or condition. [0024] In a further aspect, GEP, GEP peptides and/or atsttrin or its subunits may be utilized in methods to modulate, prevent, treat or alleviate immunological injuries, including allergies, auto-immune diseases and other such immune conditions, particularly wherein TNF/TNFR is involved or implicated. Thus, immune and/or inflammatory responses in auto-immune, allergies or other such immunological conditions may be modulated by GEP, GEP peptides and/or atsttrin or its subunits. in one such aspect, 8 auto-immune diseases, such as lupus and multiple sclerosis, may be modulated, alleviated or treated by GEP, GEP peptides and/or atsttrin or its subunits. [00251 In particular, the GEP, GEP peptides, atsttrin peptides of the present invention, including as described herein and provided in FIGURES 23 and 24 herein, their antibodies, agonists, mimics, or active fragments thereof, could be prepared in phannaceutical formulations for administration in instances wherein anti-TNF or TNF/TNFR family antagonist activity and/or therapy is appropriate, such as to treat or alleviate a TNF/TNFR mediated condition or inflammation. The GEP, GEP peptides and atsttrin peptides include exemplary GEP as set out in SEQ ID NO: I and 8, and GEP peptides or atsttrin peptides as set out in SEQ ID NO: 2 and 3-7, and variants or subunits thereof. The specificity of the GEP peptides and/or atsttrin hereof would make it possible to better manage the aftereffects of current anti-TNF and/or anti-inflammatory therapy, and would thereby make it possible to apply broadly as a general anti-TNF and/or anti-TNF family agent. [0026] The invention includes an assay system for screening of potential drugs or compounds effective to modulate TNF/TNFR activity of target mammalian cells by mimicking the activity of the GEP peptides. This aspect includes assays to screen for additional active GEP fragments, granulin/linker unit combinations, derivatives, variants and amino acid modifications effective to modulate TNF/TNFR activity in a like manner to GEP and atsttrin peptide. In one instance, the test drug or compound is administered to a cellular sample with TNFato activate TNF/TNFR activity, to determine the effect of the test drug or compound upon TNFo activity, by comparison with a control, including wherein the control is GEP, active GEP peptide(s), atsttrin. [0027] In an assay, a control quantity of the GEP, GEP peptides, atsttrin, TNFo, TNFR, or antibodies thereto, or the like may be prepared and labeled with an enzyme, a specific binding partner and/or a radioactive element, and may then be introduced into a cellular sample. After the labeled material or its binding partners) has had an opportunity to react with sites within the sample, the resulting mass may be examined by known techniques, which may vary with the nature of the label attached. in the instance where a radioactive label, such as the isotopes 3 H, ' C, 32 P, 3S, 3 6 Cl, 5Cr, 5 7 Co, "Co, 59 Fe, 90 Y, 125 J, '' I, and 'Re are used, known currently available counting procedures may be utilized. In the instance where the label is an enzyme, detection may be accomplished by any of the presently utilized colorimetric, spectrophotometric, fluorospectrophotometric, amperometric or gasometric techniques known in the art. 9 [00281 Other objects and advantages will become apparent to those skilled in the art from a review of the following description which proceeds with reference to the following illustrative drawings. BRIEF DESCRIPTION OF THE DRAWINGS [0029] FIGURE 1 depicts the structure of GEP growth factor. For the unit consensus sequence C represents cysteine, D aspartic acid, P proline, T threonine, G glycine, H histidine; dots represent any amino acids. {0030] FIGURE 2 (A) Binding of GEP to TNFR in Yeast. Each Pair of plasmids, as indicated, was co transformed into yeast strain MAV203. Yeast transformants were selected on SD-leu7trp " /his"/ura' /3AT+ plates and tested for j-galactosidase activity. The known interaction between c-Jun and c-Fos was used as a positive control, whereas the lack of interaction between Rb and lamin was used as a negative control. (B) GEP associates with TNFR2 in chondrocytes. Cell extracts prepared from human chondrocytes were incubated with control IgG, anti-TNFR2 antibodies followed by protein A-agarose. The immunoprecipitated protein complex and cell extracts (lane I, a positive control) were examined by immunoblotting with anti-GEP antibodies. [0031] FIGURE 3 depicts solid phase binding of TNFR1 extracellular domain (TNFRIECD) and TNFR2 extracellular domain (TNFR2ECD) to recombinant GEP coated on microtiter plates. [0032] FIGURE 4 (A) Schematic diagram of TNFR2 constructs used to map those of its fragments that bind to GEP. (B) P-Galactosidase assays. {00331 FIGURE 5 depicts an MTT assay. Human chondrocytes were cultured in the absence (CTR) or presence of 50ng/ml GEP (GEP) or GEP plus either lug/ml of anti-TNFRl (GEP+TNFR1 ab), anti TNFR2 (GEP+TNFR2 ab) or lug/ml of anti-IGFIR (GEP+TGF1R ab, employed as a control), and cell proliferation was analyzed using an MTT assay. [0034] FIGURE 6. GEP activates Akt and Erkl/2 pathways in human C2812 chondrocytes. Note both long-time (5min, left panel) and short-time (30 sec. right panel) exposures of the film are presented. 10 [00351 FIGURE 7 depicts expression profiling of the genes Gadd45b, JunB, KLF2, Smad7, Sox4 and Tcf8 using real-time PCR with primary wild type (B6), TNFRI -/-, and TNFR2 -/- MLE cells cultured in the presence of 50 ng/ml GEP for various time points. [0036] FIGURE 8 depicts a model for illustrating intracellular events, including signaling and target gene expression. [00371 FIGURE 9 (Left) Schematic diagram of GEP constructs used to map those of its fragments that bind to TNFR. (Right) $-Galactosidase assays. [0038] FIGURE 10 (Left) Schematic diagram of GEP constructs. (Right) p-Galactosidase assays. [0039] FIGURE 1 I (Left) Schematic diagram of GEP constructs. (Right) -Galactosidase assays. {0040] FIGURE 12 (Left) Schematic diagram of GEP constructs used to map those of its fragments that bind to TNFR. (Right) S-Galactosidase assays. [0041] FIGURE 13 (Left) Schematic diagram of GEP constructs used to map those of its fragments that bind to TNFR. (Right) $-Galactosidase assays. [00421 FIGURE 14 (Left) Schematic diagram of GEP constructs used to map those of its fragments that bind to TNFR. (Right) -Galactosidase assays. [0043] FIGURE 15 (Left) Schematic diagram of GEP constructs used to map those of its fragments that bind to TNFR. (Right) 1-Galactosidase assays. [0044] FIGURE 16 (A) Atsttrin inhibites the respiratory burst triggered by TNF in a does-dependent manner; (B) Effect of Atsttrin, Remicade and Enbrel on TNF-triggered respiratory burst. Results are means for nmol H202 produced by 1.5 x 104 cells/well in triplicate cultures. [00451 FIGURE 17 depicts a TUNEL staining assay. Rat chondrosarcoma (RCS) cells and human C2812 chondrocytes were serum-starved for 24 h to remove the effect of exogenous growth factors and 11 cytokines. Thereafter, cells were stimulated with 0.02%BSA (CTR), 50ng/ml of GEP (GEP), lOng/ml of TNF-a, or GEP plus TNF-a for 36 h. Apoptosis was measured by using an TUNEL assay kit. [00461 FIGURE 18 shows that GEP inhibits TNFca-induced metalloproteinase. Human cartilage explants were cultured in the absence or presence of either 5ng/ml of TNFasupplemented with various amount of GEP, as indicated, for 1 day in serum-free medium and real-time PCR was performed. The units are arbitrary and the leftmost bar in each group indicates a relative level of 1. 100471 FIGURE 19 A proposed model for explaining the anti-inflammation mechanisms of Atsttrin, Enbrel and Remicade. Atsttrin blocks TNF/TNFR signaling via directly binding to TNF receptors, whereas Enbrel and Remicade disturb signaling via targeting to TNF ligand. 100481 FIGURE 20 shows that Atsttrin neutralizes GEP-stimulated cell growth of cancer cells (MTT assay). RCS chondrosarcoma (left panel) and Saos-2 osteosarcoma (right panel) were cultured in the absence (CTR) or presence of GEP (200 ng/1) with or without various amounts of Atsttrin, as indicated, and cell proliferation was analyzed using an MTT assay. [0049] FIGURE 2 1 depicts IL-6 and IL- 13 levels in cell-free exudates of mice from an air-pouch acute inflammation model. Levels of IL-6 and IL- 13 are depicted in controls (CTR) and animals administered Remicade (10gg/g), GEP (10Lpg/g), and Atsttrin (10 g/g). [0050] FIGURE 22 depicts RANKL-induced osteoclastogenesis, as assessed by TRAP staining. Raw 264.7 macrophages were incubated in the presence of RANKL for 4 days, alone or with varying amounts of GEP or atsttrin. [0051] FIGURE 23 provides the amino acid sequence of human GEP (SEQ ID NO: 1) which is 593 amino acids. [0052] FIGURE 24 depicts the sequence of atsttrin peptide (SEQ ID NO: 2), and sequences of various other tested peptides (SEQ ID NOS: 3-7, respectively). 100531 FIGURE 25A and 25B depicts the (A) structure and (B) amino acid sequence of mouse GEP (SEQ ID NO: 8). In (B), the granulin units GrnA, GrnC, GmD and GrnE are underlined and indicated at each unit. 12 10054] FIGURE 26 shows that GEP associates with RANK and FAS in addition to TNFR, whereas Atsttrin specifically binds to TNFR (yeast two hybrid assay). Each pair of plasmids, as indicated, was co transformed into yeast strain MAV203. Yeast transformants were selected on SD-leu7trp' /his"/ura /3AT+ plates and tested for Q-galactosidase activity. The lack of interaction between Rb and lamin was used as a negative control (Neg. CTR). [00551 FIGURE 27 provides FastStep T M Kinetic Assay for binding of GEP (PGRN) and TNFwxto TNFRI (RI) and TNFR2 (R2). [0056] FIGURE 28A, 28B and 28C provides (A) characterization of recombinant Atsttrin. GST-Atsttrin conjugated to glutathione agarose resin and Atsttrin released by factor Xa were resolved by SDS-PAGE and the proteins were stained with Coomassie Brillian Blue R-250. (B) and (C) provide the FastStep TM Kinetic Assay for binding of Atsttrin and TNFato TNFR1 (RI) and TNFR2 (R2). [0057] FIGURE 29 depicts PathScan@ Multiplex Western Blot results against p-AKT, p-ERK and tubulin control with GEP, Atsttrin and GEP+Atsttrin. GEP activates both the Akt and Erkl/2 pathways, but Atsttrin does not. In the combination study, Atsttrin blocked GEP-mediated activation of the oncogenic p-AKT and p-ERK pathways. [0058] FIGURE 30A and 30B depicts Severity (A) (by clinical score) and Incidence (B) of arthritis in CIA mice treated with PBS (n=10), Atsttrin (n=10), or Enbrel (n=10). 10059] FIGURE 3 1 provides photographs of the front (top) and hind (bottom) paws of normal and CIA mice treated with PBS, Atsttrin, or Enbrel. [0060] FIGURE 32A and 32B depict (A) sections of each ankle of PBS-treated and Atsttrin-treated animals stained with H&E or Safranin-0 as indicated. In the H&E panel, arrows indicate tissue destruction and cell infiltration, respectively. In the Sarfanin-O panel, arrows indicate loss of matrix staining. (B) shows MicroCT images of the ankle of PBS-treated and Atsttrin-treated animals. [0061] FIGURE 33 provides TRAP staining images in PBS-treated and Atsttrin-treated animals. TRAP+ osteoclasts are depicted in red staining in the PBS-treated animal but are hardly detectable in the Atsttrin treated animal. Different magnifications are depicted for visual emphasis. 13 100621 FIGURE 34 depicts the effects of Atsttrin compared to controls PBS (negative control) and Enbrel (positive control) on proinflammatory cytokines IL- 10 and IL-6 and anti-inflammatory cytokines IL-10 and IL-13. The *, **, and *** indicate p<0.05, p<0.01 and p<0.001 respectively. 10063] FIGURE 35 provides clinical score data in CIA animals treated with either PBS, Enbrel or Atsttrin starting 25 days after collagen immunization to induce arthritis. [00641 FIGURE 36 depicts GEP and Atsttrin effects on TNF-induced nitrite production. TNFa was added to RAW264.7 cells with concomitant addition of 0.3nM, 1.5nM or 7.5nM GEP, Atsttrin or Enbrel as indicated and gM nitrite was measured. 100651 FIGURE 37 evaluates TNF-induced nuclear accumulation of NF-KB by immunofluorescence of RAW264.7 cells in the presence of TNF-o, TNF-a and GEP, or TNF-a and Atsttrin. Cells were stained for NF-KB p65, versus nuclear DAPI stain, and merged for both stains. NF-iB staining remains cytoplasmic in the presence of GEP or Atsttrin. [00661 FIGURE 38 shows fold induction of a TNF-activated NFKB reported gene in the presence of TNF-x alone, or combined with either GEP or Atsttrin at increasing concentrations. GEP and Atsttrin inhibit TNF-a mediated activation of the NFKB reported gene. DETAILED DESCRIPTION [00671 In accordance with the present invention there may be employed conventional molecular biology, microbiology, and recombinant DNA techniques within the skill of the art. Such techniques are explained fully in the literature. See, e.g., Sambrook et at, "Molecular Cloning: A Laboratory Manual" (1989); "Current Protocols in Molecular Biology" Volumes I-Ill [Ausubel, R. M,, ed. (1994)]; "Cell Biology: A Laboratory Handbook" Volumes I-III [J. E. Celis, ed. (1994))]; "Current Protocols in Immunology" Volumes I-III [Coligan, J. E., ed. (1994)]; "Oligonucleotide Synthesis" (MJ. Gait ed. 1984); "Nucleic Acid Hybridization" [B.D. Hames & S.J. Higgins eds. (1985)]; "Transcription And Translation" [B.D. Hames & S.J. Higgins, eds. (1984)]; "Animal Cell Culture" [R.I. Freshney, ed. (1986)]; "Immobilized Cells And Enzymes" [IRL Press, (1986)]; B. Perbal, "A Practical Guide To Molecular Cloning" (1984). 100681 Therefore, if appearing herein, the following terms shall have the definitions set out below, 14 [0069) The terms "Atsttrin", " Antagonist of TNF/TNFR Signaling via Targeting TNF Receptors ", "atsttrin peptide", "TNF antagonist peptide" and any variants not specifically listed, may be used herein interchangeably, and as used throughout the present application and claims refer to peptides including single or multiple proteins, particularly which are derived from or fragments of GEP and extends to those proteins having the amino acid sequence data described herein and presented in FIGURES 24 and also diagrammed in FIGURE 15, and the profile of activities and capabilities described and set forth herein and provided in the Claims. Active GEP peptides having activity as antagonist of TNF/TNFR signaling and capable of binding one or more TNF receptors, such as TNFo, are included and provided herein. The full length sequence of human GEP is provided in FIGURE 23. The full length sequence of mouse GEP is provided in FIGURE 25. Thus, TNF antagonist peptides derived from GEP sequences(s) or comprising GEP sequence(s) and having TNF and/or TNF-family antagonist activity are encompassed herein. These atsttrin peptides include and encompass fragments, variants, and derivatives of the peptides. Accordingly, proteins displaying substantially equivalent activity, and which are modifications thereof, are likewise contemplated. These modifications may be deliberate, for example, such as modifications obtained through site-directed mutagenesis, or may be accidental, such as those obtained through mutations in hosts that are producers of the complex or its named subunits. Corresponding mouse or other species or ortholog GEP sequences to the human atsttrin and active GEP peptide sequences are further contemplated. Also, the terms "Atsttrin", " Antagonist of TNF/TNFR Signaling via Targeting TNF Receptors ", "atsttrin peptide", "TNF antagonist peptide" are intended to include within their scope proteins specifically recited herein as well as all substantially homologous analogs and allelic variations. [0070] The amino acid residues described herein are preferred to be in the "L" isomeric form. However, residues in the "D" isomeric form can be substituted for any L-amino acid residue, as long as the desired fuctional property of immunoglobulin-binding is retained by the polypeptide. NIH 2 refers to the free amino group present at the amino terminus of a polypeptide. COOH refers to the free carboxy group present at the carboxy tenrinus of a polypeptide. In keeping with standard polypeptide nomenclature, J. BioL Chem., 243:3552-59 (1969), abbreviations for amino acid residues are shown in the following Table of Correspondence: TABLE OF CORRESPONDENCE SYMBOT- AMINO ACID I-Letter 3-Letter 15 Y Tyr tyrosine G GIy glycine F Phe phenylalanine M Met methionine A Ala alanine S Ser shrine 1 IHe isoleucine L Leu leucine T Thr threonine V Val valine P Pro proline K Lys lysine H His histidine Q GIn glutamine E GIu glutamic acid W Trp tryptophan R Arg arginine D Asp aspartic acid N Asn asparagine C Cys cysteine [0071] ft should be noted that all amino-acid residue sequences are represented herein by formulae whose left and right orientation is in the conventional direction of amino-terminus to carboxy-terminus. Furthermore, it should be noted that a dash at the beginning or end of an amino acid residue sequence indicates a peptide bond to a further sequence of one or more amino-acid residues. The above Table is presented to correlate the three-letter and one-letter notations which may appear alternately herein. [00721 A "replicon" is any genetic element (e.g., plasmid, chromosome, virus) that functions as an autonomous unit of DNA replication in vivo; i.e., capable of replication under its own control. [0073] A "vector" is a replicon, such as plasmid, phage or cosmid, to which another DNA segment may be attached so as to bring about the replication of the attached segment. 16 [0074] A "DNA molecule" refers to the polymeric form of deoxyribonucleotides (adenine, guanine, thymine, or cytosine) in its either single stranded form, or a double-stranded helix. This term refers only to the primary and secondary structure of the molecule, and does not limit it to any particular tertiary forms. Thus, this term includes double-stranded DNA found, inrer alia, in linear DNA molecules (e.g., restriction fragments), viruses, plasmids, and chromosomes. In discussing the structure of particular double-stranded DNA molecules, sequences may be described herein according to the normal convention of giving only the sequence in the 5' to 3' direction along the nontranscribed strand of DNA (i.e., the strand having a sequence homologous to the mRNA). [0075] An "origin of replication" refers to those DNA sequences that participate in DNA synthesis. [0076] A DNA "coding sequence" is a double-stranded DNA sequence which is transcribed and translated into a polypeptide in vivo when placed under the control of appropriate regulatory sequences. The boundaries of the coding sequence are determined by a start codon at the 5' (amino) terminus and a translation stop codon at the 31 (carboxyl) terminus. A coding sequence can include, but is not limited to, prokaryotic sequences, cDNA from eukaryotic mRNA, genomic DNA sequences from eukaryotic (e.g., mammalian) DNA, and even synthetic DNA sequences. A polyadenylation signal and transcription termination sequence will usually be located 3' to the coding sequence. [0077] Transcriptional and translational control sequences are DNA regulatory sequences, such as promoters, enhancers, polyadenylation signals, terminators, and the like, that provide for the expression of a coding sequence in a host cell. [00781 A "promoter sequence" is a DNA regulatory region capable of binding RNA polymerase in a cell and initiating transcription of a downstream (3' direction) coding sequence. For purposes of defining the present invention, the promoter sequence is bounded at its 3 t terminus by the transcription initiation site and extends upstream (51 direction) to include the minimum number of bases or elements necessary to initiate transcription at levels detectable above background. Within the promoter sequence will be found a transcription initiation site (conveniently defined by mapping with nuclease S 1), as well as protein binding domains (consensus sequences) responsible for the binding of RNA polymerase. Eukaryotic promoters will often, but not always, contain "TATA" boxes and "CAT" boxes. Prokaryotic promoters contain Shine-Dalgarno sequences in addition to the -10 and -35 consensus sequences. 17 [0079] An "expression control sequence" is a DNA sequence that controls and regulates the transcription and translation of another DNA sequence. A coding sequence is "under the control" of transcriptional and translational control sequences in a cell when RNA polymerase transcribes the coding sequence into mRNA, which is then translated into the protein encoded by the coding sequence. [0080] A "signal sequence" can be included before the coding sequence. This sequence encodes a signal peptide, N-terminal to the polypeptide, that communicates to the host cell to direct the polypeptide to the cell surface or secrete the polypeptide into the media, and this signal peptide is clipped off by the host cell before the protein leaves the cell. Signal sequences can be found associated with a variety of proteins native to prokaryotes and eukaryotes. [00814 The term "oligonucleotide," as used herein in referring to the probe of the present invention, is defined as a molecule comprised of two or more ribonucleotides, preferably more than three. Its exact size will depend upon many factors which, in turn, depend upon the ultimate function and use of the oligonucleotide. [0082] The term "primer" as used herein refers to an oligonucleotide, whether occurring naturally as in a purified restriction digest or produced synthetically, which is capable of acting as a point of initiation of synthesis when placed under conditions in which synthesis of a primer extension product, which is complementary to a nucleic acid strand, is induced, i.e., in the presence of nucleotides and an inducing agent such as a DNA polymerase and at a suitable temperature and pH. The primer may be either single stranded or double-stranded and must be sufficiently long to prime the synthesis of the desired extension product in the presence of the inducing agent. The exact length of the primer will depend upon many factors, including temperature, source of primer and use of the method. For example, for diagnostic applications, depending on the complexity of the target sequence, the oligonucleotide primer typically contains 15-25 or more nucleotides, although it may contain fewer nucleotides. [0083] The primers herein are selected to be "substantially" complementary to different strands of a particular target DNA sequence. This means that the primers must be sufficiently complementary to hybridize with their respective strands. Therefore, the primer sequence need not reflect the exact sequence of the template. For example, a non-complementary nucleotide fragment may be attached to the 5' end of the primer, with the remainder of the primer sequence being complementary to the strand. Alternatively, non-complementary bases or longer sequences can be interspersed into the primer, 18 provided that the primer sequence has sufficient complementarity with the sequence of the strand to hybridize therewith and thereby form the template for the synthesis of the extension product. [0084] As used herein, the terms "restriction endonucleases" and "restriction enzymes" refer to bacterial enzymes, each of which cut double-stranded DNA at or near a specific nucleotide sequence. [00853 A cell has been "transformed" by exogenous or heterologous DNA when such DNA has been introduced inside the cell. The transforming DNA may or may not be integrated (covalently linked) into chromosomal DNA making up the genome of the cell. In prokaryotes, yeast, and mammalian cells for example, the transforming DNA may be maintained on an episomal element such as a plasmid. With respect to eukaryotic cells, a stably transformed cell is one in which the transforming DNA has become integrated into a chromosome so that it is inherited by daughter cells through chromosome replication. This stability is demonstrated by the ability of the eukaryotic cell to establish cell lines or clones comprised of a population of daughter cells containing the transforming DNA. A "clone" is a population of cells derived from a single cell or common ancestor by mitosis. A "cell line" is a clone of a primary cell that is capable of stable growth in vitro for many generations. [0086] A "heterologous" region of the DNA construct is an identifiable segment of DNA within a larger DNA molecule that is not found in association with the larger molecule in nature. Thus, when the heterologous region encodes a mammalian gene, the gene will usually be flanked by DNA that does not flank the mammalian genomic DNA in the genome of the source organism. Another example of a heterologous coding sequence is a construct where the coding sequence itself is not found in nature (e.g., a cDNA where the genomic coding sequence contains introns, or synthetic sequences having codons different than the native gene). Allelic variations or naturally-occurring mutational events do not give rise to a heterologous region of DNA as defined herein. [0087] Two DNA sequences are "substantially homologous" when at least about 75% (preferably at least about 80%, and most preferably at least about 90 or 95%) of the nucleotides match over the defined length of the DNA sequences. Sequences that are substantially homologous can be identified by comparing the sequences using standard software available in sequence data banks, or in a Southern hybridization experiment under, for example, stringent conditions as defined for that particular system. Defining appropriate hybridization conditions is within the skill of the art. See, e.g., Maniatis et al., supra; DNA Cloning, Vols. I & II, supra; Nucleic Acid Hybridization, supra. It should be appreciated 19 that also within the scope of the present invention are DNA sequences encoding which code for a having the same amino acid sequence as SEQ ID NO:, but which are degenerate to SEQ ID NO:. By "degenerate to" is meant that a different three-letter codon is used to specify a particular amino acid. It is well known in the art that certain codons can be used interchangeably to code for each specific amino acid. For example, and not by limitation, Leucine (Leu or L) may be encoded by any of UUA or UUG or CUU or CUC or CUA or CUG, and Serine (Ser or S) may be encoded by any of UCU or UCC or UCA or UCG or AGU or AGC. It should be understood that these exemplary codons specified are for RNA sequences. The corresponding codons for DNA have a T substituted for U. [0088] A DNA sequence is "operatively linked" to an expression control sequence when the expression control sequence controls and regulates the transcription and translation of that DNA sequence. The tern "operatively linked" includes having an appropriate start signal (e.g., ATG) in front of the DNA sequence to be expressed and maintaining the correct reading frame to permit expression of the DNA sequence under the control of the expression control sequence and production of the desired product encoded by the DNA sequence. If a gene that one desires to insert into a recombinant DNA molecule does not contain an appropriate start signal, such a start signal can be inserted in front of the gene. [0089] The tern "standard hybridization conditions" refers to salt and temperature conditions substantially equivalent to 5 x SSC and 65 0 C for both hybridization and wash. However, one skilled in the art will appreciate that such "standard hybridization conditions" are dependent on particular conditions including the concentration of sodium and magnesium in the buffer, nucleotide sequence length and concentration, percent mismatch, percent formamide, and the like. Also important in the determination of "standard hybridization conditions" is whether the two sequences hybridizing are RNA RNA, DNA-DNA or RNA-DNA. Such standard hybridization conditions are easily determined by one skilled in the art according to well known formulae, wherein hybridization is typically 10-20NC below the predicted or determined T, with washes of higher stringency, if desired. [0090] As used herein, "pg" means picogram, "ng" means nanogram, "ug" or "pg" mean microgram, "mg" means milligram, "ul" or 'L" mean microliter, "ml" means milliliter, "r means liter. [0091] Mutations can be made in GEP, GEP peptides, and/or atsttrin such an amino acid is substituted or modified or such that a particular codon is changed to a codon which codes for a different amino acid. Such a mutation is generally made by making the fewest nucleotide changes possible. A substitution 20 mutation of this sort can be made to change an amino acid in the resulting protein in a non-conservative manner (i.e., by changing the codon from an amino acid belonging to a grouping of amino acids having a particular size or characteristic to an amino acid belonging to another grouping) or in a conservative manner (i.e., by changing the codon from an amino acid belonging to a grouping of amino acids having a particular size or characteristic to an amino acid belonging to the same grouping). Such a conservative change generally leads to less change in the structure and function of the resulting protein. A non conservative change is more likely to alter the structure, activity or function of the resulting protein. The present invention should be considered to include sequences containing conservative changes which do not significantly alter the activity or binding characteristics of the resulting protein or peptide. The present invention should be considered to include sequences containing conservative and/or non conservative changes which do not significantly alter the activity or binding characteristics of the resulting protein or peptide. [0092] The following is one example of various groupings of amino acids: Amino acids with nonpolar R groups: Alanine, Valine, Leucine, Isoleucine, Proline, Phenylalanine, Tryptophan, Methionine Amino acids with uncharged polar R groups: Glycine, Serine, Threonine, Cysteine, Tyrosine, Asparagine, Glutamine Amino acids with charged polar R groups (negatively charged at Ph 6.0): Aspartic acid, Glutamic acid Basic amino acids (positively charged at pH 6.0): Lysine, Arginine, Histidine (at pH 6.0) Another grouping may be those amino acids with phenyl groups: Phenylalanine, Tryptophan, Tyrosine. [0093] Another grouping may be according to molecular weight (i.e., size of R groups): Glycine 75 Alanine 89 Serine 105 Proline 115 Valine 117 Threonine 119 Cysteine 121 Leucine 131 Isoleucine 131 Asparagine 132 21 Aspartic acid 133 Glutamine 146 Lysine 146 Glutamic acid 147 Methionine 149 Histidine (at pH 6.0) 155 Phenylalanine 165 Arginine 174 Tyrosine 181 Tryptophan 204 [0094] Particularly preferred substitutions are: - Lys for Arg and vice versa such that a positive charge may be maintained; - GIu for Asp and vice versa such that a negative charge may be maintained; - Ser for Thr such that a free -OH can be maintained; and - GIn for Asn such that a free NH, can be maintained. {0095] Amino acid substitutions may also be introduced to substitute an amino acid with a particularly preferable property. For example, a Cys may be introduced a potential site for disulfide bridges with another Cys. A His may be introduced as a particularly "catalytic" site (i.e., His can act as an acid or base and is the most common amino acid in biochemical catalysis). Pro may be introduced because of its particularly planar structure, which induces. -turns in the protein's structure. 100963 Two amino acid sequences are "substantially homologous" when at least about 70% of the amino acid residues (preferably at least about 80%, and most preferably at least about 90 or 95%) are identical, or represent conservative substitutions. [0097] An "antibody" is any immunoglobulin, including antibodies and fragments thereof, that binds a specific epitope. The term encompasses polyclonal, monoclonal, and chimeric antibodies, the last mentioned described in further detail in U.S. Patent Nos. 4,816,397 and 4,816,567. 22 [0098] An "antibody combining site" is that structural portion of an antibody molecule comprised of heavy and light chain variable and hypervariable regions that specifically binds antigen. [0099] The phrase "antibody molecule" in its various grammatical forms as used herein contemplates both an intact immunoglobulin molecule and an immunologically active portion of an immunoglobulin molecule. [001001 Exemplary antibody molecules are intact immunoglobulin molecules, substantially intact immunoglobulin molecules and those portions of an immunoglobulin molecule that contains the paratope, including those portions known in the art as Fab, Fab', F(ab') 2 and F(v), which portions are preferred for use in the therapeutic methods described herein. [00101] Fab and F(ab'), portions of antibody molecules are prepared by the proteolytic reaction of papain and pepsin, respectively, on substantially intact antibody molecules by methods that are well known. See for example, U.S. Patent No. 4,342,566 to Theofilopolous et al, Fab' antibody molecule portions are also well-known and are produced from F(ab') 2 portions followed by reduction of the disulfide bonds linking the two heavy chain portions as with mercaptoethanol, and followed by alkylation of the resulting protein mercaptan with a reagent such as iodoacetamide. An antibody containing intact antibody molecules is preferred herein. [00102] The phrase "monoclonal antibody" in its various grammatical forms refers to an antibody having only one species of antibody combining site capable of immunoreacting with a particular antigen. A monoclonal antibody thus typically displays a single binding affinity for any antigen with which it immunoreacts. A monoclonal antibody may therefore contain an antibody molecule having a plurality of antibody combining sites, each immunospecific for a different antigen; e.g., a bispecific (chimeric) monoclonal antibody. [00103] The phrase "pharmaceutically acceptable" refers to molecular entities and compositions that are physiologically tolerable and do not typically produce an allergic or similar untoward reaction, such as gastric upset, dizziness and the like, when administered to a human. [00104] The tern "therapeutically effective amount" means that amount of a drug, compound, peptide, or pharmaceutical agent that will elicit the biological or medical response of a subject that is 23 being sought by a medical doctor or other clinician. The phrase "therapeutically effective amount" is used herein to include an amount sufficient to prevent, and preferably reduce by at least about 30 percent, more preferably by at least 50 percent, most preferably by at least 90 percent, a clinically significant change in the S phase activity of a target cell or cellular mass, or other feature of pathology such as for example, elevated blood pressure, fever or white cell count as may attend its presence and activity. [00105) The term "preventing" or "prevention" refers to a reduction in risk of acquiring or developing a disease or disorder (i.e., causing at least one of the clinical symptoms of the disease not to develop) in a subject that may be exposed to a disease-causing agent, or predisposed to the disease in advance of disease onset. 100106] The tenn "prophylaxis" is related to and encompassed in the term "prevention", and refers to a measure or procedure the purpose of which is to prevent, rather than to treat or cure a disease. Non-limiting examples of prophylactic measures may include the administration of vaccines; the administration of low molecular weight heparin to hospital patients at risk for thrombosis due, for example, to immobilization; and the administration of an anti-malarial agent such as chloroquine, in advance of a visit to a geographical region where malaria is endemic or the risk of contracting malaria is high. [00107] The tern "solvate" means a physical association of a compound useful in this invention with one or more solvent molecules. This physical association includes hydrogen bonding. In certain instances the solvate will be capable of isolation, for example when one or more solvent molecules are incorporated in the crystal lattice of the crystalline solid. "Solvate" encompasses both solution-phase and isolable solvates. Representative solvates include hydrates, ethanolates and methanolates. [00108] The term "subject" includes humans and other mammals. [00109] The term "treating" or "treatment" of any disease or disorder refers, in one embodiment, to ameliorating the disease or disorder (i.e., arresting the disease or reducing the manifestation, extent or severity of at least one of the clinical symptoms thereof)- In another embodiment 'treating' or 'treatment' refers to ameliorating at least one physical parameter, which may not be discernible by the subject. In yet another embodiment, 'treating' or 'treatment' refers to modulating the disease or disorder, either physically, (e.g., stabilization of a discernible symptom), physiologically, (e.g., stabilization of a physical 24 parameter), or both. In a further embodiment, 'treating' or 'treatment' relates to slowing the progression of the disease. [00110} The term "disease characterized by inflammation", "inflammatory disease" refers to a disease which involves, results at least in part from, or includes inflammation. The term includes, but is not limited to, exemplary diseases selected from rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, juvenile idiopathic arthritis, psoriasis, inflammatory bowel diseases, Chrohn's disease, ulcerative colitis, uveitis, inflammatory lung diseases, chronic obstructive pulmonary disease. [00111] The term "inflammatory mediators" refers to mediators which enhance, initiate or facilitate an inflammatory reaction or an inflammatory response, and may be selected from the following: Cytokines (e.g. TNFalpha, IL3, IL4, IL5, IL13, GM-CSF), chemokines (e.g. MDC, CCL19, CCL20, CCL21, MIP-I alpha), Prostaglandins (e.g. PGD2), Leukotrienes (e.g. LTB4, LTC4, LTD4), metalloproteases, chymase, tryptase, growth factors (e.g. VEGF). [00112] Despite the prevalence of arthritic diseases, their precise etiology, pathogenesis, and progression remain beyond our understanding. Evidence is accumulating that demonstrates the significance of inflammatory cytokines and growth factors in the pathological processes of arthritis. The isolation of the growth factors that regulate chondrocytes and arthritis, and inhibitors that antagonize the actions of cytokines, are therefore of great importance from both a pathophysiological and a therapeutic standpoint. Granulin/epithelin precursor (GEP) has been previously recognized as a novel chondrogenic growth factor that plays an essential role in cartilage formation, including as described in WO 2008/094687 A2 and by Liu and colleagues (Xu, K et al (2007) J Biol Chem 282(15):l 1347-1 1355). [00113] The present invention demonstrates that the growth factor GEP directly associates with TNV receptors and acts as a naturally-occurring antagonist of TNFc, the central inflammatory cytokine in arthritis. Thus, the purpose of this invention is to extend the understanding of the molecular mechanisms by which growth factors and cytokines control cartilage development and arthritis, and to provide GEP, particularlyits derived and active peptide(s), particularly atsttrin, and/or derivatives or variants thereof as novel anti-TNF/TNFR modulators. Atsttrin is demonstrated herein to bind and antagonize TNF, and alter TNF/TNFR signaling. GEP is demonstrated herein to bind RANK a TNF family member. Thus, GEP, GEP peptides, and/or atsttrin are applicable and useful in therapeutic interventions for various kinds of 25 TNF-related diseases, including inflammatory conditions such as arthritis, bone diseases, and cancer conditions, such as osteoarthritis, osteoporosis, and osteosarcoma. [001141 In vivo animal models of TNF/TNFR family mediated diseases or conditions may be utilized by the skilled artisan to further or additionally evaluate, assess, screen and/or verify the GEP, GEP peptides and/or atsttrin of the invention or agents or compounds identified in or in accordance with the present invention, including further assessing TNF/TNFR modulation in vivo. Animal models are readily available to demonstrate the applicability of recombinant GEP and GEP-derived peptide(s), atsttrin in mediating, alleviating or controlling the development and progression of TNF/TNFR mediated diseases or conditions, inflammatory conditions, immune diseases or conditions (including allergies an auto-immune diseases), bone diseases or conditions, or other possible targeted conditions. Animal models or studies include those described and detailed herein and in the examples. TNFatransgenic mice develop arthritis and provide a useful tool for evaluating the efficacy of novel therapeutic strategies for rheumatoid arthritis. Animal models include, but are not limited to, ulcerative colitis models, multiple sclerosis models (including EAE, lysolecithin-induced), arthritis models, allergic asthma models, airway inflammation models, psoriasis models, and acute inflammation models. The EAE animal model of multiple sclerosis provides an acute or chronic-relapsing, acquired, inflammatory and demyclinating autoimmune disease. Allergy models may be utilized as models of immunological injury and conditions. Osteoarthritis models include for example experimental osteoarthritis induced in rabbits after sectioning of the knee anterior cruciate ligament and in rats after tear of the medial collateral ligament. Appropriate bone disease, bone injury, and/or osteoporosis models are also known and available to one of skill in the art. [001151 The invention includes use and applications of GEP, GEP peptides, atsttrin, and/or derivatives thereof for prevention, treatment or alleviation of rheumatoid arthritis and osteoarthritis. The invention includes use and applications of GEP, GEP peptides, atsttrin, and/or derivatives thereof for prevention, treatment or alleviation of TNF-related diseases, including inflammatory conditions, immune conditions including auto-immune diseases, bone diseases and cancer. TNF-related diseases include rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, psoriasis, inflammatory bowel diseases, Chrohn's disease, ulcerative colitis, uveitis, inflammatory lung diseases, chronic obstructive pulmonary disease, multiple sclerosis, osteoporosis, osteosarcoma. The invention includes use and applications of GEP, GEP peptides, atsttrin, and/or derivatives thereof for prevention, treatment or alleviation of and/or for specific therapeutic intervention of inflammatory disorders by delivering precisely the required anti 26 TNF/TNFR effect. The invention includes use and applications of GEP, GEP peptides, atstrrin, and/or derivatives thereof for facilitating or mediating tissue repair. The invention includes use and applications of GEP, GEP peptides, atsttrin, and/or derivatives thereof for prevention, treatment or alleviation of immunological injury and conditions, including allergies and auto-immune diseases, such as lupus and multiple sclerosis. The invention includes use and applications of GEP, GEP peptides, atsttrin, and/or derivatives thereof for prevention, treatment or alleviation of cancer and tumor or cancer cell growth, including in GEP and/or TNF/TNFR mediated cancers or other such hyperproliferative disorders. [00116] The possibilities both diagnostic and therapeutic that are raised by the existence of TNF antagonist peptides, including GEP, GEP peptides and/or atsttrin as described herein, derive from the fact that the peptides participate in direct and causal protein-protein interaction with TNF and serve to block, inhibit, antagonize, interfere with TNF/TNFR activity and/or signaling. Thus, the present invention contemplates pharmaceutical intervention in the cascade of reactions in which TNF/TNFR and/or TNF family/TNF family R is implicated, to modulate the activity, signal(s), and/or conditions initiated, facilitated or mediated thereby, including but not limited to inflammatory diseases and conditions. [00117] The GEP, GEP peptides and/or atsttrin as described herein or other ligands or agents exhibiting either mimicry therewith or cognate TNF antagonism, may be prepared in pharmaceutical compositions, with a suitable carrier and at a strength effective for administration by various means to a patient experiencing an adverse medical condition associated with TNF/TNFR signaling, such as an inflammatory condition or disease. A variety of administrative techniques may be utilized, among them parenteral techniques such as subcutaneous, intravenous and intraperitoneal injections, catheterizations and the like. Average quantities of the GEP, GEP peptides and/or atsttrin as described herein or their subunits may vary and in particular should be based upon the recommendations and prescription of a qualified physician or veterinarian. [001181 Also, antibodies including both polyclonal and monoclonal antibodies, and drugs that modulate the production or activity of the GEP, GEP peptides and/or atsttrin as described herein and/or their subunits may possess certain diagnostic applications and may for example, be utilized for the purpose of detecting and/or measuring conditions such as TNF-mediated diseases, inflammatory conditions, infections, cancer, or the like. For example, the GEP peptides and/or atsttrin may be used to produce both polyclonal and monoclonal antibodies to themselves in a variety of cellular media, by known techniques such as the hybridoma technique utilizing, for example, fised mouse spleen 27 lymphocytes and myeloma cells. Likewise, small molecules that mimic or antagonize the activity(ies) of the GEP, GEP peptides and/or atsttrin as described herein of the invention may be discovered or synthesized, and may be used in diagnostic and/or therapeutic protocols. [00119] The general methodology for making monoclonal antibodies by hybridomas is well known. Immortal, antibody-producing cell lines can also be created by techniques other than fusion, such as direct transformation of B lymphocytes with oncogenic DNA, or transfection with Epstein-Barr virus. See, e.g., M. Schreier et al., "Hybridoma Techniques" (1980); Hammerling et al., "Monoclonal Antibodies And T-cell Hybridomas" (1981); Kennett et al., "Monoclonal Antibodies" (1980); see also U.S. Patent Nos. 4,341,761; 4,399,121; 4,427,783; 4,444,887; 4,451,570; 4,466,917; 4,472,500; 4,491,632; 4,493,890. [00120] Preferably, the anti- GEP, GEP peptides and/or atsttrin antibody used in the diagnostic methods of this invention is an affinity purified polyclonal antibody. More preferably, the antibody is a monoclonal antibody (nAb). in addition, it is preferable for the anti- GEP, GEP peptides and/or atsttrin antibody molecules used herein be in the form of Fab, Fab, F(ab') 2 or F(v) portions of whole antibody molecules. [001211 The present invention further contemplates therapeutic compositions useful in practicing the therapeutic methods of this invention. A therapeutic composition includes a biologically compatible composition. A subject therapeutic composition includes, in admixture, a pharnaceutically acceptable excipient (carrier) and one or more of a GEP, GEP peptide(s) and/or atsttrin polypeptide analog thereof or fragment thereof, as described herein as an active ingredient. In a preferred embodiment, the composition comprises the present GEP, GEP peptide(s) and/or atsttrin, and may comprise composition comprising one or more GEP granulin and one or more linker unit, or any one or more of the GEP peptide(s) or atsttrin, including as set out in the figures herein, including FIGURES 23 and 24 and as provided in SEQ ID NOS: 1-8. [00122] The peptides and compositions of the invention include those GEP peptides, including atsttrin, which are based on the human GEP sequence, including as set out in FIGURES 23 and 34, as well as variants thereof having one or more or a few or many substitutions, wherein the binding and activity profiles of the variant(s) are retained when compared to the atsttrin GEP peptide. In as much as GEP peptides from various animals or mammals, including humans, are known, these sequences provide 28 alternative amino acid sequences and variants of potential use in the compositions and methods of the invention, including by substitution of some of the atsttrin human peptide amino acids. Mouse GEP sequence is provided herein in FIGURE 25. GEP sequences for various animals are publicly known and disclosed and would be available for evaluation and assessment in the methods and compositions of the invention, and their corresponding and correlating amino acids suitable for evaluation and use as variants of the GEP peptides herein. GEP sequences are available and herein incorporated by reference as follows: rat (Genbank accession AAA 16903.1, CAA44 198.1), mouse (Genbank accession P28798.2, BAE35389.1, NP_032201.2), Sumatran orangutan (Genbank accession NP_001 126689.1), crab-eating macague (Genbank accession BAEO 1796.1), horse (Genbank accession XPoo148979 1.1), cattle (Genbank accession NP-oo 1070482.1), rabbit (Genbank accession XP_0027 19228.1), pig (Genbank accession NPoo 1038043.1), chimpanzee (Genbank accession XP_511549.2) and opossum (Genbank accession XPJ)0 1374870.1). 1001231 A peptide, analog or active fragment can be formulated into the therapeutic composition as neutralized pharmaceutically acceptable salt forms. Pharmaceutically acceptable salts include the acid addition salts (formed with the free amino groups of the polypeptide or antibody molecule) and which are formed with inorganic acids such as, for example, hydrochloric or phosphoric acids, or such organic acids as acetic, oxalic, tartaric, mandelic, and the like. Salts formed from the free carboxyl groups can also be derived from inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides, and such organic bases as isopropylamine, trimethylamine, 2-ethylamino ethanol, histidine, procaine, and the like. 1001241 The therapeutic peptide-, analog- or active fragment-containing compositions are conventionally administered intravenously, as by injection of a unit dose, for example. The term "unit dose" when used in reference to a therapeutic composition of the present invention refers to physically discrete units suitable as unitary dosage for humans, each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect in association with the required diluent; i.e., carrier, or vehicle. [001251 The compositions are administered in a manner compatible with the dosage formulation, and in a therapeutically effective amount. The quantity to be administered depends on the subject to be treated, capacity of the subject's immune system to utilize the active ingredient, and degree of inhibition or neutralization of TNF/TNFR activity desired. Precise amounts of active ingredient required to be 29 administered depend on thejudgment of the practitioner and are peculiar to each individual. However, suitable dosages may range from about 0.1 to 20, preferably about 0.5 to about 10, and more preferably one to several, milligrams of active ingredient per kilogram body weight of individual per day and depend on the route of administration. Suitable regimes for initial administration and booster shots are also variable, but are typified by an initial administration followed by repeated doses at one or more hour intervals by a subsequent injection or other administration. Alternatively, continuous intravenous infusion sufficient to maintain concentrations often nanomolar to ten micromolar in the blood are contemplated. [00126] A particular biologically compatible composition is an aqueous solution that is buffered using, e.g., Tris, phosphate, or HEPES buffer, containing salt ions. Usually the concentration of salt ions will be similar to physiological levels. Biologically compatible solutions may include stabilizing agents and preservatives. In a more preferred embodiment, the biocompatible composition is a pharmaceutically acceptable composition. Such compositions can be formulated for administration by topical, oral, parenteral, intranasal, subcutaneous, and intraocular, routes. Parenteral administration is meant to include intravenous injection, intramuscular injection, intraarterial injection or infusion techniques. The composition may be administered parenterally in dosage unit formulations containing standard, well known non-toxic physiologically acceptable carriers, adjuvants and vehicles as desired. [00127] A particular embodiment of the present composition invention is a pharmaceutical composition comprising a therapeutically effective amount of GEP, GEP peptide(s) and/or atsttrin as described hereinabove, in admixture with a pharmaceutically acceptable can-ier. Another particular embodiment is a pharmaceutical composition for the treatment or prevention of a disease characterized by TNF/TNFR activity including infections, allograft reactions, inflammation, allergic and autoimmune diseases, and cancer, or a susceptibility to said disease, comprising an effective amount of the GEP, GEP peptide(s) and/or atsttrin, its pharmaceutically acceptable salts, hydrates, solvates, or prodrugs thereof in admixture with a phannaceutically acceptable carrier. A further particular embodiment is a pharmaceutical composition for the treatment or prevention of a disease involving inflammation, or a susceptibility to the condition, comprising an effective amount of the GEP, GEP peptide(s) and/or atsttrin, its pharmaceutically acceptable salts, hydrates, solvates, or prodrugs thereof in admixture with a pharmaceutically acceptable carrier. 30 [0001] The compositions of the invention may include GEP, GEP peptides, atsttrin, and/or derivatives thereof in combination with one or more agents suitable for the alleviation, prevention or treatment of inflammation, immunological conditions, hyperproliferative conditions, and/or cancer. The compositions of the invention may include GEP, GEP peptides, atsttrin, and/or derivatives thereof in combination with one or more of an anti-inflammatory agent, an anti-cancer agent, or an immunodulatory agent. More generally these anti-cancer agents may be tyrosine kinase inhibitors or phosphorylation cascade inhibitors, post-translational modulators, cell growth or division inhibitors (e.g. anti-mitotics), inhibitors or signal transduction inhibitors. Other treatments or therapeutics may include the administration of suitable doses of pain relief drugs such as non-steroidal anti-inflammatory drugs (e.g. aspirin, paracetamol, ibuprofen or ketoprofen) or opiates such as morphine, or anti-emetics. In addition, the composition may incorporate or be administered with immune modulators, such as interleukins, tumor necrosis factor (TNF) or other growth factors, colony stimulating factors, cytokines or hormones. [00128] Pharmaceutical compositions for oral administration can be formulated using pharmaceutically acceptable carriers well known in the art in dosages suitable for oral administration. Such carriers enable the pharmaceutical compositions to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions, and the like, for ingestion by the patient. Pharmaceutical compositions for oral use can be prepared by combining active compounds with solid excipient, optionally grinding a resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries, if desired, to obtain tablets or dragee cores. Suitable excipients are carbohydrate or protein fillers, such as sugars, including lactose, sucrose, mannitol, or sorbitol; starch from corn, wheat, rice, potato, or other plants; cellulose, such as methyl cellulose, hydroxypropylmethyl-cellulose, or sodium carboxymethyl-cellulose; gums including arabic and tragacanth; and proteins such as gelatin and collagen. If desired, disintegrating or solubilizing agents may be added, such as the cross-linked polyvinyl pyrrolidone, agar, alginic acid, or a salt thereof, such as sodium alginate. Dragee cores may be used in conjunction with suitable coatings, such as concentrated sugar solutions, which may also contain gum arabic, tale, polyvinylpyrrolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dyestuffs or pigments may be added to the tablets or dragee coatings for product identification or to characterize the quantity of active compound, i.e., dosage. [00129] Pharmaceutical preparations that can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a coating, such as glycerol or sorbitol. Push 31 fit capsules can contain active ingredients mixed with filler or binders, such as lactose or starches, lubricants, such as talc or magnesium stearate, and, optionally, stabilizers. In soft capsules, the active compounds may be dissolved or suspended in suitable liquids, such as fatty oils, liquid, or liquid polyethylene glycol with or without stabilizers. [00130] Preferred sterile injectable preparations can be a solution or suspension in a non-toxic parenterally acceptable solvent or diluent. Examples of pharmaceutically acceptable carriers are saline, buffered saline, isotonic saline (e.g. monosodium or disodium phosphate, sodium, potassium; calcium or magnesium chloride, or mixtures of such salts), Ringer's solution, dextrose, water, sterile water, glycerol, ethanol, and combinations thereof 1,3-butanediol and sterile fixed oils are conveniently employed as solvents or suspending media. Any bland fixed oil can be employed including synthetic mono- or di glycerides. Fatty acids such as oleic acid also find use in the preparation of injectables. [00131) The agents or compositions of the invention may be combined for administration with or embedded in polymeric carriers), biodegradable or bionimetic matrices or in a scaffold. The carrier, matrix or scaffold may be of any material that will allow composition to be incorporated and expressed and will be compatible with the addition of cells or in the presence of cells. Particularly, the carrier matrix or scaffold is predominantly non-immunogenic and is biodegradable. Examples of biodegradable materials include, but are not limited to, polyglycolic acid (PGA), polylactic acid (PLA), hyaluronic acid, catgut suture material, gelatin, cellulose, nitrocellulose, collagen, albumin, fibrin, alginate, cotton, or other naturally-occurring biodegradable materials. It may be preferable to sterilize the matrix or scaffold material prior to administration or implantation, e.g., by treatment with ethylene oxide or by gamma irradiation or irradiation with an electron beam. In addition, a number of other materials may be used to form the scaffold or framework structure, including but not limited to: nylon (polyamides), dacron (polyesters), polystyrene, polypropylene, polyacrylates, polyvinyl compounds (e.g., polyvinylchloride), polycarbonate (PVC), polytetrafluorethylene (PTFE, teflon), thermanox (TPX), polymers of hydroxy acids such as polylactic acid (PLA), polyglycolic acid (PGA), and polylactic acid-glycolic acid (PLGA), polyorthoesters, polyanhydrides, polyphosphazenes, and a variety of polyhydroxyalkanoates, and combinations thereof. Matrices suitable include a polymeric mesh or sponge and a polymeric hydrogel. In the particular embodiment, the matrix is biodegradable over a time period of less than a year, more particularly less than six months, most particularly over two to ten weeks. The polymer composition, as well as method of manufacture, can be used to determine the rate of degradation. For example, mixing increasing amounts of polylactic acid with polyglycolic acid decreases the degradation time. Meshes of 32 polyglycolic acid that can be used can be obtained commercially, for instance, from surgical supply companies (e.g., Ethicon, NJ). In general, these polymers are at least partially soluble in aqueous solutions, such as water, buffered salt solutions, or aqueous alcohol solutions that have charged side groups, or a monovalent ionic salt thereof. [00132] The composition medium can also be a hydrogel, which is prepared from any biocompatible or non-cytotoxic homo- or hetero-polymer, such as a hydrophilic polyacrylic acid polymer that can act as a drug absorbing sponge. Certain of them, such as, in particular, those obtained from ethylene and/or propylene oxide are commercially available. A hydrogel can be deposited directly onto the surface of the tissue to be treated, for example during surgical intervention. [00133] The active expression-inhibiting agents may also be entrapped in microcapsules prepared, for example, by interfacial polymerization, for example, hydroxymethylcellulose or gelatin-microcapsules and poly-(methylmethacylate) microcapsules, respectively, in colloidal drug delivery systems (for example, liposomes, albumin microspheres, microemulsions, nano-particles and nanocapsules) or in macroemulsions. Such techniques are disclosed in Remington's Pharmaceutical Sciences (1980) 16th edition, Osol, A. Ed. [00134] Sustained-release preparations may be prepared. Suitable examples of sustained-release preparations include semi-penneable matrices of solid hydrophobic polymers containing the antibody, which matrices are in the form of shaped articles, e.g. films, or microcapsules. Examples of sustained release matrices include polyesters, hydrogels (for example, poly(2-hydroxyethyl-methacrylate), or poly(vinylalcohol)), polylactides (U.S. Pat. No. 3,773,919), copolymers of L-glutamic acid and gamma ethyl-L-glutamate, non-degradable ethylene-vinyl acetate, degradable lactic acid-glycolic acid copolymers such as the LUPRON DEPOTTM (injectable microspheres composed of lactic acid-glycolic acid copolymer and leuprolide acetate), and poly-D-(-)-3-hydroxybutyric acid. While polymers such as ethylene-vinyl acetate and lactic acid-glycolic acid enable release of molecules for over 100 days, certain hydrogels release proteins for shorter time periods. When encapsulated antibodies remain in the body for a long time, they may denature or aggregate as a result of exposure to moisture at 37*C, resulting in a loss of biological activity and possible changes in immunogenicity. Rational strategies can be devised for stabilization depending on the mechanism involved. For example, if the aggregation mechanism is discovered to be intermolecular S-S bond formation through thio-disulfide interchange, stabilization may 33 be achieved by modifying sulfhydryl residues, lyophilizing from acidic solutions, controlling moisture content, using appropriate additives, and developing specific polymer matrix compositions. [00135] As defined above, therapeutically effective dose means that amount of protein, polynucleotide, peptide, or its antibodies, agonists or antagonists, which ameliorate the symptoms or condition. Therapeutic efficacy and toxicity of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., ED 50 (the dose therapeutically effective in 50% of the population) and LD, 5 (the dose lethal to 50% of the population). The dose ratio of toxic to therapeutic effects is the therapeutic index, and it can be expressed as the ratio, LD 5 /ED50. Pharmaceutical compositions that exhibit large therapeutic indices are preferred. The data obtained from cell culture assays and animal studies are used in formulating a range of dosage for human use. The dosage of such compounds lies preferably within a range of circulating concentrations that include the

ED

5 0 with little or no toxicity. The dosage varies within this range depending upon the dosage form employed, sensitivity of the patient, and the route of administration. [00136] For any compound, the therapeutically effective dose can be estimated initially either in cell culture assays or in animal models, usually mice, rabbits, dogs, or pigs. The animal model is also used to achieve a desirable concentration range and route of administration. Such information can then be used to determine useful doses and routes for administration in humans. The exact dosage is chosen by the individual physician in view of the patient to be treated. Dosage and administration are adjusted to provide sufficient levels of the active moiety or to maintain the desired effect. Additional factors which may be taken into account include the severity of the disease state, age, weight and gender of the patient; diet, desired duration of treatment, method of administration, time and frequency of administration, drug combination(s), reaction sensitivities, and tolerance/response to therapy. Long acting pharmaceutical compositions might be administered every 3 to 4 days, every week, or once every two weeks depending on half-life and clearance rate of the particular formulation. [00137] The pharmaceutical compositions according to this invention may be administered to a subject by a variety of methods. They may be added directly to target tissues, complexed with cationic lipids, packaged within liposomes, or delivered to target cells by other methods known in the art. Localized administration to the desired tissues may be done by direct injection, transdermal absorption, catheter, infusion pump or stent. The DNA, DNA/vehicle complexes, or the recombinant virus particles are locally administered to the site of treatment. Alternative routes of delivery include, but are not limited 34 to, intravenous injection, intramuscular injection, subcutaneous injection, aerosol inhalation, oral (tablet or pill form), topical, systemic, ocular, intraperitoneal and/or intrathecal delivery. [001381 Alternatively, or additionally, a polynucleotide encoding the GEP, GEP peptide(s) and/or atsttrin may be particularly included within a vector. The polynucleic acid is operably linked to signals enabling expression of the nucleic acid sequence and is introduced into a cell utilizing, preferably, recombinant vector constructs, which will express the antisense nucleic acid once the vector is introduced into the cell. A variety of viral-based systems are available, including adenoviral, retroviral, adeno associated viral, lentiviral, herpes simplex viral or a sendaiviral vector systems, and all may be used to introduce and express polynucleotide sequence for the expression-inhibiting agents or the polynucleotide expressing the TARGET polypeptide in the target cells. [001391 Particularly, the viral vectors used in the methods of the present invention are replication defective. Such replication defective vectors will usually pack at least one region that is necessary for the replication of the virus in the infected cell. These regions can either be eliminated (in whole or in part), or be rendered non-functional by any technique known to a person skilled in the art. These techniques include the total removal, substitution, partial deletion or addition of one or more bases to an essential (for replication) region. Such techniques may be perfonned in vitro (on the isolated DNA) or in situ, using the techniques of genetic manipulation or by treatment with mutagenic agents. Preferably, the replication defective virus retains the sequences of its genome, which are necessary for encapsidating, the viral particles. [00140] In a preferred embodiment, the viral element is derived from an adenovirus. Preferably, the vehicle includes an adenoviral vector packaged into an adenoviral capsid, or a functional part, derivative, and/or analogue thereof. Adenovirus biology is also comparatively well known on the molecular level. Many tools for adenoviral vectors have been and continue to be developed, thus making an adenoviral capsid a preferred vehicle for incorporating in a library of the invention. An adenovirus is capable of infecting a wide variety of cells, However, different adenoviral serotypes have different preferences for cells. To combine and widen the target cell population that an adenoviral capsid of the invention can enter in a preferred embodiment, the vehicle includes adenoviral fiber proteins from at least two adenoviruses. Preferred adenoviral fiber protein sequences are serotype 17, 45 and 5 1. Techniques or construction and expression of these chimeric vectors are disclosed in US 2003/0180258 and US 2004/0071660, hereby incorporated by reference. 35 [00141] In a preferred embodiment, the nucleic acid derived from an adenovirus includes the nucleic acid encoding an adenoviral late protein or a functional part, derivative, and/or analogue thereof. An adenoviral late protein, for instance an adenoviral fiber protein, may be favorably used to target the vehicle to a certain cell or to induce enhanced delivery of the vehicle to the cell. Preferably, the nucleic acid derived from an adenovirus encodes for essentially all adenoviral late proteins, enabling the formation of entire adenoviral capsids or functional parts, analogues, and/or derivatives thereof. Preferably, the nucleic acid derived from an adenovirus includes the nucleic acid encoding adenovirus E2A or a functional part, derivative, and/or analogue thereof. Preferably, the nucleic acid derived from an adenovirus includes the nucleic acid encoding at least one E4-region protein or a functional part, derivative, and/or analogue thereof, which facilitates, at least in part, replication of an adenoviral derived nucleic acid in a cell. The adenoviral vectors used in the examples of this application are exemplary of the vectors useful in the present method of treatment invention. [001421 Certain embodiments of the present invention use retroviral vector systems. Retroviruses are integrating viruses that infect dividing cells, and their construction is known in the art. Retroviral vectors can be constructed from different types of retrovirus, such as, MoMuLV urineie Moloney leukemia virus") MSV ("murine Moloney sarcoma virus"), HaSV ("Harvey sarcoma virus"); SNV ("spleen necrosis virus"); RSV ("Rous sarcoma virus") and Friend virus. Lentiviral vector systems may also be used in the practice of the present invention. [00143] In other embodiments of the present invention, adeno-associated viruses ("AAV") are utilized. The AAV viruses are DNA viruses of relatively small size that integrate, in a stable and site specific manner, into the genome of the infected cells. They are able to infect a wide spectrum of cells without inducing any effects on cellular growth, morphology or differentiation, and they do not appear to be involved in human pathologies. [00144] In the vector construction, the polynucleotide agents of the present invention may be linked to one or more regulatory regions. Selection of the appropriate regulatory region or regions is a routine matter, within the level of ordinary skill in the art. Regulatory regions include promoters, and may include enhancers, suppressors, etc. 36 [00145] Promoters that may be used in the expression vectors of the present invention include both constitutive promoters and regulated (inducible) promoters. The promoters may be prokaryotic or eukaryotic depending on the host. Among the prokaryotic (including bacteriophage) promoters useful for practice of this invention are lac, lacZ, T3, T7, lambda Pr' P,, and trp promoters. Among the eukaryotic (including viral) promoters useful for practice of this invention are ubiquitous promoters (e.g. HPRT, vimentin, actin, tubulin), therapeutic gene promoters (e.g. MDR type, CFTR, factor VIII), tissue-specific promoters, including animal transcriptional control regions, which exhibit tissue specificity and have been utilized in transgenic animals, e.g. immunoglobulin gene control region which is active in lymphoid cells (Grosschedl, et al. (1984) Cell 38:647-58; Adames, et al. (1985) Nature 318:533-8; Alexander, et al. (1987) Mol. Cell. Biol. 7:1436-44), and mouse mammary tumor virus control region which is active in testicular, breast, lymphoid and mast cells (Leder, et al. (1986) Cell 45:485-95). [00146] Other promoters which may be used in the practice of the invention include promoters which are preferentially activated in dividing cells, promoters which respond to a stimulus (e.g. steroid hormone receptor, retinoic acid receptor), tetracycline-regulated transcriptional modulators, cytomegalovirus immediate-early, retroviral LTR, metallothionein, SV-40, Ela, and MLP promoters. Further promoters which may be of use in the practice of the invention include promoters which are active and/or expressed in dendritic cells. 100147] Additional vector systems include the non-viral systems that facilitate introduction of polynucleotide agents into a patient. For example, a DNA vector encoding a desired sequence can be introduced in vivo by lipofection. Synthetic cationic lipids designed to limit the difficulties encountered with liposome-mediated transfection can be used to prepare liposomes for in vivo transfection of a gene encoding a marker (Feigner, et. al. (1987) Proc. Natl. Acad Sci. USA 84:7413-7); see Mackey, et al. (1988) Proc. Natl. Acad. Sci. USA 85:8027-31; Ulmer, et al. (1993) Science 259:1745-8). The use of cationic lipids may promote encapsulation of negatively charged nucleic acids, and also promote fusion with negatively charged cell membranes (Feigner and Ringold, (1989) Nature 337:387-8). Particularly useful lipid compounds and compositions for transfer of nucleic acids are described in International Patent Publications WO 95/18863 and WO 96/17823, and in U.S. Pat. No. 5,459,127. The use of lipofection to introduce exogenous genes into the specific organs in vivo has certain practical advantages and directing transfection to particular cell types would be particularly advantageous in a tissue with cellular heterogeneity, for example, pancreas, liver, kidney, and the brain. Lipids may be chemically coupled to other molecules for the purpose of targeting. Targeted peptides, e.g., hormones or 37 neurotransmitters, and proteins for example, antibodies, or non-peptide molecules could be coupled to liposomes chemically. Other molecules are also useful for facilitating transfection of a nucleic acid in vivo, for example, a cationic oligopeptide (e.g., International Patent Publication WO 95/21931), peptides derived from DNA binding proteins (e.g., International Patent Publication WO 96/25508), or a cationic polymer (e.g., International Patent Publication WO 95/2193 1). [00148] It is also possible to introduce a DNA vector in vivo as a naked DNA plasmid (see U.S. Pat. Nos. 5,693,622, 5,589,466 and 5,580,859). Naked DNA vectors for therapeutic purposes can be introduced into the desired host cells by methods known in the art, e.g., transfection, electroporation, microinjection, transduction, cell fusion, DEAE dextran, calcium phosphate precipitation, use of a gene gun, or use of a DNA vector transporter (see, e.g., Wilson, et al. (1992) J. Biol, Chem. 267:963-7; Wu and Wu, (1988) J. Biol. Chem. 263:14621-4; Hartmut, et al. Canadian Patent Application No. 2,012,31 1, filed Mar. 15, 1990; Williams, et al (1991). Proc. Natl. Acad. Sci. USA 88:2726-30). Receptor-mediated DNA delivery approaches can also be used (Curiel, et al. (1992) Hum. Gene Ther. 3:147-54; Wu and Wu, (1987) J. Biol. Chem. 262:4429-32). 38 [00149] In addition, the present invention envisions preparing GEP peptides and/or atsttrin peptides that have distinct or different structural or sequence properties, and the use of peptidomimetics, and peptidomimetic bonds, such as ester bonds, to prepare additional peptides or agents with the properties of the GEP peptides and/or atsttrin, i.e. capable of inhibiting or antagonizing TNF and TNF/TNFR. In another embodiment, a peptide may be generated that incorporates a reduced peptide bond, i.e., R r-CH 2 -NH-R, where RI and R 2 are amino acid residues or sequences. A reduced peptide bond may be introduced as a dipeptide subunit. Such a molecule would be resistant to peptide bond hydrolysis, e.g., protease activity. Such peptides would provide antagonists with unique function and activity, such as extended half-lives in vivo due to resistance to metabolic breakdown, or protease activity. Furthermore, it is well known that in certain systems constrained peptides show enhanced functional activity (Hruby, 1982, Life Sciences 3 \ A %9-\99; Hruby et al., 1990, Biochein J. 268:249-262. [00150] A constrained, cyclic or rigidized peptide may be prepared synthetically, provided that in at least two positions in the sequence of the peptide an amino acid or amino acid analog is inserted that provides a chemical functional group capable of cross-linking to constrain, cyclist or rigidize the peptide after treatment to form the cross-link. Cyclization will be favored when a turn-inducing amino acid is incorporated. Examples of amino acids capable of cross-linking a peptide are cysteine to form disulfide, aspartic acid to form a lactone or a lactase, and a chelator such as y-carboxyl-glutamic acid (Gla) (Bachem) to chelate a transition metal and form a cross-link. Protected y-carboxyl glutamic acid may be prepared by modifying the synthesis described by Zee-Cheng and Olson (1980, Biophys. Biochem. Res. Commnnm. 94:1 128-1 132). A peptide in which the peptide sequence comprises at least two amino acids capable of cross-linking may be treated, e.g., by oxidation of cysteine residues to form a disulfide or addition of a metal ion to form a chelate, so as to cross-link the peptide and form a constrained, cyclic or rigidized peptide. [00151] The present invention contemplates strategies to systematically prepare cross-links. For example, if four cysteine residues are incorporated in the peptide sequence, different protecting groups may be used (Hiskey, 1981, in The Peptides: Analysis, Synthesis, Biology, Vol. 3, Gross and Meienhofer, eds., Academic Press: New York, pp. 137-167; Ponsanti et al., 1990, Tetrahedron 46:8255-8266). The first pair of cysteine may be deprotected and oxidized, then the second set may be deprotected and oxidized. In this way a defined set of disulfide cross-links may be formed. Alternatively, a pair of cysteine and a pair of collating amino acid analogs may be incorporated so that the cross-links are of a different chemical nature. 39 [00152) The following non-classical amino acids may be incorporated in the peptide in order to introduce particular conformational motifs: 1,2,3,4-tetrahydroisoquinoline-3-carboxylate (Kazmierski et al., 1991,J. Am. Chem. Soc. 113:2275-2283); (2S,3S)-methyl-phenylalanine, (2S,3R)-methyl phenylalanine, (2R,3S)-methyl-phenylalanine and (2R,3R)-methyl-phenylalanine (Kazmierski and Hruby, 1991, Tetrahedron Lett.); 2-aminotetrahydronaphthalene-2-carboxylic acid (Landis, 1989, Ph.D. Thesis, University of Arizona); hydroxy- l,2,3,4-tetrahydroisoquinoline-3_carboxylate (Miyake et al., 1989, J. TakedaRes. Labs. 43:53-76); j-carboline (D and L) (Kazmierski, 1988, Ph.D. Thesis, University of Arizona); HIC (histidine isoquinoline carboxylic acid) (Zechel et al., 1991, Int. J. Pep. Protein Res. 43); and HIC (histidine cyclic urea) (Dharanipragada). [001531 The following amino acid analogs and peptidomimetics may be incorporated into a peptide to induce or favor specific secondary structures: LL-Acp (LL-3-amino-2_propenidone-6 carboxylic acid), a f-turn inducing dipeptide analog (Kemp et al., 1985, J. Org. Chem. 50:5834-5838); $ sheet inducing analogs (Kemp et al., 1988, Tetrahedron Lett. 29:5081-5082); -turn inducing analogs (Kemp et al., 1988, Tetrahedron Lett. 29:5057-5060); g-helix inducing analogs (Kemp et al., 1988, Tetrahedron Lett. 29:4935-4938); 7-turn inducing analogs (Kemp et al., 1989,J. Org. Chemn. 54:109:1 15); and analogs provided by the following references: Nagai and Sato, 1985, Tetrahedron Lett. 26:647_650; DiMaio et al., 1989, J. Chem. Soc. Perkin Trans, p. 1687; also a Gly-Alaturn analog (Kahn et al., 1989, Tetrahedron Lett. 30:2317); aide bond isostere (Jones et al., 1988, Tetrahedron Lett. 29:3853-3856); tretrazol (Zabrocki et al., 1988, J. Am. Chem. Soc. 110:5875-5880); DTC (Samanen et al., 1990, Int. J. Protein Pep. Res. 35:501:509); and analogs taught in Olson et al., 1990, J. Am. Chem. Sci. 112:323-333 and Garvey et al., 1990,J. Org. Chem. 56:436. Conformational ly restricted mimetics of beta turns and beta bulges, and peptides containing them, are described in U.S. Patent No. 5,440,013, issued August 8, 1995 to Kahn. [001541 The present invention further provides for modification or derivatization of the polypeptide or peptide of the invention. These modifications may serve to alter or increase the stability, activity, half-life of the polypeptide or peptide of the invention. Modifications of peptides are well known to one of ordinary skill, and include phosphorylation, carboxymethylation, and acylation. Modifications may be effected by chemical or enzymatic means. In another aspect, glycosylated or fatty acylated peptide derivatives may be prepared. Preparation of glycosylated or fatty acylated peptides is well known in the art. Fatty acyl peptide derivatives may also be prepared. For example, and not by way of limitation, 40 a free amino group (N-terminal or lysyl) may be acylated, e.g., myristoylated. In another embodiment an amino acid comprising an aliphatic side chain of the structure -(CH 2 )nCH 3 may be incorporated in the peptide. This and other peptide-fatty acid conjugates suitable for use in the present invention are disclosed in U.K. Patent GB-8809162.4, International Patent Application PCT/AU89/00166, and reference 5, supra. Addition of carbohydrate moieties and the preparation and use of glycosylated analogs of the peptides of the invention is also contemplated, including for improved biological and physical properties such as proteolytic stability and in vivo activity. [00155] Chemical Moieties For Derivaization. Derivatives of the peptides (including variants, analogs and active fragments thereof) of the present invention are further provided. Such derivatives encompass and include derivatives to enhance activity, solubility, effective therapeutic concentration, and transport across the blood brain barrier. Further encompassed derivatives include the attachment of moieties or molecules which are known to interact with TNF/TNFR, to target TNF/TNFR or expressing cells, or to have anti-inflammatory activity. The chemical moieties may be N-terminally or C-terminally attached to the peptides of the present invention. Chemical moieties suitable for derivatization may be, for instance, selected from among water soluble polymers. The polymer selected can be water soluble so that the component to which it is attached does not precipitate in an aqueous environment, such as a physiological environment. Preferably, for therapeutic use of the end-product preparation, the polymer will be pharmaceutically acceptable. The polymer may be branched or unbranched. One skilled in the art will be able to select the desired polymer based on such considerations as whether the polymer/component conjugate will be used therapeutically, and if so, the desired dosage, circulation time, resistance to proteolysis, and other considerations. For the present component or components, these may be ascertained using the assays provided herein. [00156] The water soluble polymer may be selected from the group consisting of, for example, polyethylene glycol, copolymers of ethylene glycol/propylene glycol, carboxymethylcellulose, dextran, polyvinyl alcohol, polyvinyl pyrrolidone, poly-1, 3-dioxolane, poly-l,3,6-trioxane, ethylene/maleic anhydride copolymer, polyaminoacids (either homopolymers or random copolymers), and dextran or poly(n-vinyl pyrrolidone)polyethylene glycol, propropylene glycol homopolymers, prolypropylene oxide/ethylene oxide co-polymers, polyoxyethylated polyols and polyvinyl alcohol. Polyethylene glycol propionaldenhyde may have advantages in manufacturing due to its stability in water. 41 [00157J The polymer may be of any suitable molecular weight, and may be branched or unbranched. For polyethylene glycol, the preferred molecular weight is between about 2kDa and about 100kDa (the term "about" indicating that in preparations of polyethylene glycol, some molecules will weigh more, some less, than the stated molecular weight) for ease in handling and manufacturing. Other sizes may be used, depending on the desired therapeutic profile (e.g., the duration of sustained release desired, the effects, if any on biological activity, the ease in handling, the degree or lack of antigenicity and other known effects of the polyethylene glycol to a therapeutic protein or analog). [00158] The number of polymer molecules so attached may vary, and one skilled in the art will be able to ascertain the effect on function. One may mono-derivative, or may provide for a di-, tri-, tetra- or some combination of derivatization, with the same or different chemical moieties (e.g., polymers, such as different weights of polyethylene glycols). The proportion of polymer molecules to component or components molecules will vary, as will their concentrations in the reaction mixture. In general, the optimum ratio (in terms of efficiency of reaction in that there is no excess unreacted component or components and polymer) will be determined by factors such as the desired degree of derivatization (e.g., mono, di-, tri-, etc.), the molecular weight of the polymer selected, whether the polymer is branched or unbranched, and the reaction conditions. [00159] The polyethylene glycol molecules (or other chemical moieties) should be attached to the component or components with consideration of effects on functional or antigenic domains of the protein. There are a number of attachment methods available to those skilled in the art, e.g., EP 0 401 384 herein incorporated by reference (coupling PEG to G-CSF), see also Malik et al, 1992, Exp. HematoL 20: 1028 1035 (reporting pegylation of GM-CSF using tresyl chloride). For example, polyethylene glycol may be covalently bound through amino acid residues via a reactive group, such as, a free amino or carboxyl group. Reactive groups are those to which an activated polyethylene glycol molecule may be bound. The amino acid residues having a free amino group include lysine residues and the terminal amino acid residues; those having a free carboxyl group include aspartic acid residues glutamic acid residues and the C-terminal amino acid residue. Sulfhydrl groups may also be used as a reactive group for attaching the polyethylene glycol molecule(s). Preferred for therapeutic purposes is attachment at an amino group, such as attachment at the N-terminus or lysine group. [00160] More particularly the present invention provides derivatives which are fusion proteins comprising the peptides of the present invention or fragments thereof Thus peptides of the present 42 invention and fragments thereof can be "modified" i.e., placed in a fusion of chimeric peptide or protein, or labeled, e.g., to have an N-terminal FLAG-tag. In a particular embodiment a peptide can be modified by linkage or attachment to a marker protein such as green fluorescent protein as described in U.S. Patent No. 5,625,048 filed April 29, 1997 and WO 97/26333, published July 24, 1999 (each of which are hereby incorporated by reference herein in their entireties). In one such embodiment, a chimeric peptide can be prepared, e.g., a glutathione-S-transferase (GST) fusion protein, a maltose-binding (MPB) protein fusion protein, or a poly-histidine-tagged fusion protein, for expression in a eukaryotic cell. Expression of the peptide of the present invention as a fusion protein can facilitate stable expression, or allow for purification based on the properties of the fusion partner. For example, GST binds glutathione conjugated to a solid support matrix, MBP binds to a maltose matrix, and poly-histidine chelates to a Ni-chelation support matrix. The fusion protein can be eluted from the specific matrix with appropriate buffers, or by treating with a protease specific for a cleavage site usually engineered between the peptide and the fusion partner (e.g., GST, MBP, or poly-His). Alternatively the chimeric peptide may contain the green fluorescent protein, and be used to determine the intracellular localization of the peptide in the cell. [00161] The invention also includes derivatives wherein at least one of the attached chemical moieties is a molecule having multiple sites for peptide attachment and capable of binding at least two of said peptides simultaneously to generate a multimeric peptide structure. This derivative has the effect of increasing the available local concentration of the carbohydrate epitope mimic peptide(s) of the present invention. Alternatively, or in addition, such moieties can function in providing a stable scaffold to retain the peptide in place for activity, thereby reducing or preventing diffusion or degradation. More particularly, such molecule is selected from the group of BSA, ovalbumin, human senum allbumin, polyacrylamide, beads and synthetic fibers (biodegradable and non-biodegradable). [00162] The carbohydrate epitope mimic peptide of the present invention may be prepared and utilized as monomers, dimers, multimers, heterodirners, heteromultimers, etc. Presentation or administration of the peptide in multimeric form may result in enhanced activity or otherwise increased modulation of the activity mediated by the peptide(s), including TNF antagonistic activity and/or inhibiting TNF/TNFR signaling and activity. The peptide monomer could be produced in a variety of ways. The peptide of the present invention can be synthesized using a protein synthesizer and utilizing methods well known in the art and as described hereinabove, incorporating amino acid modifications, analogs, etc. as hereinabove described. In addition, the DNA sequence of the peptide can be inserted into an expression vector such as pSE (Invitrogen) or pCDNA3 (Invitrogen) for production in bacterial or 43 mammalian cell expression systems. Insect or yeast expression systems could also be used. Purification of the peptide could be facilitated by the addition of a tag sequence such as the 6-Histidine tag which binds to Nickel-NTA resins. These tag sequences are often easily removed by the addition of a protease specific sequence following the tag. Dimers and multimers of the peptide can be produced using a variety of methods in the art. The DNA sequence of a dimer or multimer could also be inserted into an expression system such as bacteria or mammalian cell systems. This could produce molecules such as Met-FLHTRLFV)xwhere x = 2, 3, 4, ... etc. It may be necessary to include a short flexible spacer (Gly Gly-Gly-Gly-Ser)3 between the peptide or peptidomimetic to increase its effectiveness. Dimers and multimers can also be generated using crosslinking reagents such as Disuccinimidyl suberate (DSS) or Dithoiobis (succinimidyl propionate) (DSP). These reagents are reactive with amino groups and could crosslink the peptide through free amine groups at the arginine residues and the free amine group at the N-terminus. Dimers and multimers can also be formed using affinity interactions between biotin and avidin, Jun and Fos, and the Fc region of antibodies. The purified peptide can be biotinylated and mixed with factors that are known to form strong protein-protein interactions. The peptide or peptidomimetic could be linked to the regions in Jun and Fos responsible for dimer formation using crosslinkers such as those mentioned above or using molecular techniques to create a peptide-Jun/Fos molecule. When the Jun and Fos peptide hybrids are mixed, dimer formation would result. In addition, production of a peptide-Fe hybrid could also be produced. When expressed in mammalian cells, covalent disulfide bonds form through cysteines in the Fe region and dimer formation would result. Heterodimers and heteromultimers of the peptide could also be produced. This would generate possible multifunctional molecules where parts of the whole molecule are responsible for producing a multitude of effects, such as anti-TNF and/or anit-inflammatory and/or cell growth modulating effects. The same technologies as those listed above could be used to generate these multifunctional molecules. Molecular techniques could be used to insert the carbohydrate epitope mimic peptide into a protein at the DNA level. This insertion could take place at the N- or C- terminus, or in the middle of the protein molecule. Heterodimers could be formed using peptide/Fe or peptide/June or Fos hybrid molecules. When mixed with other Fc or Jun/Fos containing hybrids dimer formation would result producing heterodimers. Crosslinking reagents could also be used to link the peptide to heterodimers. Lastly, biotinylation of the peptide along with biotinylation of other molecules could be used to create multimers. Mixing of these components with avidin could create large multifunctional complexes, where each of the four biotin binding sites of the avidin molecule is occupied by a different biotinylated molecule. 44 1001631 In one aspect the present invention provides a method of preventing and/or treating a disease characterized by, mediated by or facilitated by TNF/TNFR activity and/or signaling and/or a diseases characterized by inflammation, immune injury, and cancer, said method comprising administering to a subject a therapeutically effective amount of a TNF antagonist peptide as disclosed herein. In a particular embodiment, the peptide is selected from GEP, GEP peptide(s) and/or atsttrin. In a particular embodiment, the disease is selected from rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, psoriasis, inflammatory bowel diseases, Chrohn's disease, ulcerative colitis, uveitis, inflammatory lung diseases, and chronic obstructive pulmonary disease. In an embodiment, the disease may be an immunological disorder or condition, including allergies and auto-immune diseases, such as lupus and multiple sclerosis. In an aspect, the disease may be cancer, including a GEP-mediated cancer or TNF/TNFR mediated cancer. 1001641 The invention also relates to the use of a peptide as described above and herein for the preparation of a medicament for treating or preventing a disease characterized by, mediated by or facilitated by TNF/TNFR activity and/or signaling and/or diseases characterized by inflammation, and cancer. In a particular embodiment, the disease is characterised by inflammation. In a particular embodiment of the present invention the disease is selected from rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, psoriasis, inflammatory bowel diseases, Chrohn's disease, ulcerative colitis, uveitis, inflammatory lung diseases, and chronic obstructive pulmonary disease. [001651 The invention may be better understood by reference to the following non-limiting Examples, which are provided as exemplary of the invention. The following examples are presented in order to more fully illustrate the preferred embodiments of the invention and should in no way be construed, however, as limiting the broad scope of the invention. EXAMPLE 1 GEP Binds b and Antagonizes TNFccReceptors (TNFR) 45 . 1001661 Modern methods of global analysis of protein-protein interactions followed by biological assessment have led to powerful ways of identifying novel proteins not previously associated with the pathogenesis of a particular disease or organ system. Through a functional genetic screen, we have now discovered that GEP, a novel mediator in chondrogenesis and arthritis, associates with TNFR. This extends our understanding of the action of growth factors and cytokines in cartilage biology and their application to treatment of cartilage disorders and arthritic conditions. In particular, our studies shed light on a naturally occurring antagonist of the central proinflammatory cytokine TNFO, and provide insights into the degradative events that occur in patients with arthritic disorders. The identification and manipulation of growth factors that regulate the chondrogenic potential of mesenchymal stem cells and act as an inhibitors of a central proinflammatory cytokine can be used to optimize the therapeutic application in cartilage disorders and connective tissue disorders. Important long-term goals of this work are (1) define the role of GEP, TNFO, as well as interaction and function interplay among them in regulating skeletal biology and related diseases; and (2) to recruit GEP, specially GEP-derived peptides, to develop new anti-TNF/TNFR therapeutic interventions for various kinds of TNF-related diseases, including arthritis. 1001671 Our global screens led to the isolation of several novel GEP-binding partners and among them TNF receptors (TNFR) are of great interest to us. Subsequent studies showed that GEP directly bound to the extracellular domains of TNFR, and GEP-stimulated signaling and target gene expression in chondrocytes strictly depends on TNFR. The fact that both GEP and TNFq bind to TNFR raised the possibility that the binding of GEP to TNFR may block the association of TNFq and its receptors, i.e, GEP may act as a naturally-occurring antagonist of TNFa Indeed, GEP dramatically inhibits TNF induced inflammation response and chondrocyte apoptosis. f00168] TNFR2 identified as a GEP-associated receptor: Taking into account the biological properties of GEP, it has been hypothesized that GEP could act through "classic" membrane receptor(s), as do other known growth factors. Thus far, a functional receptor has not been identified. In a search for GEP-associated proteins we screened a yeast two-hybrid (Y2H) cDNA library using the construct pDBleu-GEP(a.a.2 1-588) encoding GEP lacking signal peptide as bait, and isolated 24 positive clones. Sequencing data from these clones showed that two of them were cell surface TNFR2 (TNFRSF IB/CD 120b; Accession #NM_1 30426). 46 [00169] GEP binds to TNFR2 in yeast and in chondrocytes: To verify the interaction between GEP and TNFR2 in yeast the plasmid encoding the GEP linked to GaWDBD and the plasmid encoding an N-terminal truncated mutant of TNFR2 (a.a. 26-567) fused to the VP 16AD were co-transformed into the yeast cells. Like the c-Jun/c-Fos pair, which is known to interact and used as a positive control, our assays indicated that COMP interacts with GEP in yeast, based on the activity of J-galactosidase (FIGURE 2A). To determine whether these two proteins interacted in primary human chondrocytes, a coimmunoprecipitation (Co-IP) assay was performed (FIGURE 28). Briefly, the cell extracts prepared from isolated human chondrocytes were incubated with either anti-TNFR2 antibody or control IgG, and the immunoprecipitated complexes were subjected to a reducing SDS-PAGE and detected with anti-GEP antibodies. A specific GEP band was present in the immunoprecipitated complexes brought down by anti-TNFR2 (lane 3), but not by control IgG (lane 2) antibodies, demonstrating that GEP specifically binds to the TNFR2 in primary human chondrocytes. 1001701 Direct binding of GEP to the extracellular domains of TNFRI and TNFR2: Since there is remarkable amino acid similarity between extracellular domains of TNFR1 and TNFR2, we next determined whether GEP directly binds to TNFR1 and TNFR2 using solid-phase binding assay with recombinant GEP and extracellular domains of TNFR1 and TNFR2 (R & D System) (FIGURE 3). Briefly, microtiter plates were coated with 500 ng of purified GEP in 100 p1l of TBS buffer (50mM Tris/HCI, 150mM NaCl, pH 7

.

4 ). After blocking, various amounts (5-500ng) of extracellular domain of TNFRl (TNFRIECD, left panel) or extracellular domain of TNFR2 (TNFR2ECD, right panel) were added to each well, and bound protein from the liquid phase was detected by antibody against TNFRI or TNFR2, followed by a secondary antibody conjugated with horseradish peroxidase. As shown in FIGURE 3, GEP demonstrated dose-dependent binding and saturation to the liquid-phase TNFRIECD and TNFR2ECD. [00171] Cysteine-rich domain (CRD) of TNFR2 is sufficient for binding to GEP: Various deletion mutants of TNFR2 were generated and tested in yeast two-hybrid assay for their ability to interact with GEP. Results from filter-based P-galactosidase assays (FIGURE 4) of all these mutants are summarized in FIGURE 4A. Our conclusion from this set of experiments is that each CRD (i.e. CR01, CRD2, CRD3 or CRD4) of TNFR2 is sufficient for its interaction with GEP. 47 [001721 Anti-TNFR specific blocking antibodies or recombinant extracellular domains of TNFR abolishes GEP-stimulated chondrocyte proliferation: The findings that GEP binds to the TNRf, together with our recent report that GEP has potent mitogenic effects on human chondrocytes [49], led us to determine whether GEP-stimulated chondrocyte proliferation depends on the TNFR. Human chondrocytes were cultured in the absence (CTR) or presence of 50ng/ml GEP (GEP) or GEP plus either lug/ml of anti-TNFRl (GEP+TNFR1 ab; SC-7895 is against extracellular domain of TNFRI), anti-TNFR2 (GEP+TNFR2 ab; SC- 12751 is against extracellular domain of TNFR2) or lug/ml of anti IGFlR (GEP+TGF IR ab, employed as a control), and cell proliferation was analyzed using an MTT assay (FIGURE 5 left panel). As expected, GEP potently stimulated chondrocyte proliferation, and this GEP-stimulated cell proliferation was largely blocked by either anti-TNFRI or anti-TNFR2 antibody whereas anti-IGFIR did not demonstrate any blocking effects on GEP action. [001731 Since GEP directly associates with the extracellular domains of TNFR, we next examined whether recombinant extracellular domains of TNFR will affect GEP action in chondrocyte proliferation via competing with endogenous TNFR for interacting with GEP. As shown in the right panel of FIGURE 5, chondrocyte proliferation induced by 50ng/ml GEP was completely abolished by recombinant extracellular domain of either TNFR (RIECD, 25ng/ml) or TNFR2 (R2ECD, 25ng/ml). Taken together, these results indicate that GEP-mediated chondrocyte proliferation strictly depends on the TNFR activity. [00174] GEP activates Akt and Erkl/2 pathways in chondrocytes: We next sought to analyze GEP-activated signaling in chondrocytes using The PathScan@ Multiplex Western Cocktail I (Cell Signaling) that allows us to simultaneously detect levels of phospho-p90RSK, phospho-Akt, phosphop44/42 MAPK (Erkl/2) and phospho-S6 ribosomal protein on a single membrane. Human C2812 chondrocytes (provided by Dr. Mary B. Goldring) were starved for 24 hours and treated with 50ng/ml of GEP for various time points and cell lysates were analyzed using The PathScan® Multiplex Western Cocktail 1. As shown in FIGURE 6, GEP specifically activated Akt and p44/p42 (Erkl/2) pathways in chondrocytes. 100175] GEP-mediated activation of target genes depends on TNFR: 48 To identify GEP downstream molecules, we performed genome-wide DNA chip analysis. Total RNA was isolated from human C2812 chondrocytes treated with 50ng/mil of GEP for various time points and analyzed by microarray analysis (Affymetrix, Santa Clara, CA). Approximately 40 genes were determined to be upregulated (over 2-fold) following GEP treatment as determined by hierarchical clustering [53]. Interestingly, the GEP-inducible genes, including Gadd45 P, JunB, KLF2, Samd7, Sox4 and Tcf8, are also known to be activated by the TGF3 Psubfamily [54-56]. We next determined whether activation of these genes by GEP depends on TNFR, expression profiling of these genes were examined using real-time PCR with primary wildtype (B6), TNFRl-/-, and TNFR2-/- MLE cells cultured in presence of 50 ng/ml GEP for various time points (FIGURE 7). GEP clearly activated these genes in wildtype MLE cells; however, GEP largely lost these inductions in either TNFRl-/- or TNFR2-/- cells, indicating that GEP induction of its target genes depends on TNFR1 and TNFR2. Interestingly, both TNFR1 and TNFR2 appear to be important for GEP-stimulated gene expression. [00176] A proposed model for illustrating the TNFa- and GEP-induced Intracellular events: GEP binds to the CRD of TNFR (FIGURE 4), as does TNFa [57]. Thus there exists a reciprocal inhibition of binding to TNFR between GEP and TNFo, An intriguing question is why GEP and TNFo, that use the same receptor TNFR, induce opposite responses. As illustrated in FIGURE 8, TNFatrimmer binds to the extracellular domains of TNF receptors and induces 1) a strong activation of the stress-related JNK and moderate response of the p38, and 2) activation of NF-kB pathway [58], whereas GEP potently activates Erkl/2 and moderately Akt signaling (FIGURE 6 and FIGURE 8). A number of GEP-activated genes, including Sox4, Smad7, JunB, Gadd45 P, Tcf8, are also activated by the TGF subfamily, and GEP-mediated gene activation depends on TNFR (FIGURE 7). EXAMPLE 2 Discovery of Atsttrin (Antagonist of TNF/TNFR Signaling via Targeting 'NF Receptors) 1001771 To identify the peptide(s) of GEP required for binding to TNF receptors, a series of GEP mutants (i.e. C-terminal deletions, N-tenninal deletions, individual granulin unit, individual linker, as well as various combinations) were expressed in a yeast expression plasmid. Briefly, cDNA segments encoding the series of GEP mutants were amplified by PCR and cloned in-frame into the Sall/Notl sites of pDBleu (Life Technologies) yeast expression vector. The generated plasmids and pPC86-TNFR 49 encoding extracellular domain of TNFR1 /R2 was cotransformed into the yeast MaV203 strain containing three reporter genes, His+, Ura+, and LacZ (Life Technologies), and transformants was examined for f3galactosidase. Results from filter-based f3-galactosidase assays (right panel in figures FIGURE 9 through FIGURE 15) of all these mutants are summarized in the left panel of these figures. As revealed in FIGURE 9, results from a series of C-tenninal deletions indicated that deletions from the C-terminal of GEP reduced the binding affinity to TNFR and finally totally lost binding activity. This is also true for series of N-terminal deletion mutants (FIGURE 10). [001781 Neither a single granulin unit (A, B, C, D, E, F, or G; FIGURE 11) nor a single linker unit (P1, P2, P3, P4, PS, P6 or P7; FIGURE 12) could bind to TNFR, suggesting that the binding region of GEP may span one or more granulin unit and linker. To examine this hypothesis, we first linked each granulin unit with its 3'-linker and found that granulin unit F plus linker P3 exhibited weak binding to TNFR (FIGURE 13). Next, we linked each granulin unit with its 5'-linker and found that P4 plus granulin unit A showed weak binding to TNFR, and also that P5 plus unit C showed weak binding to TNFR (FIGURE 14). These findings led us to test the binding of various combinations of half unit of A, C and F plus P3, P4 and P5 to TNFR. As revealed in FIGURE 15, 1/2A+P3+P4+1/2C+P5+l/2F demonstrated strong binding to TNFR. This GEP derived peptide is now referred to as Atsttrin. EXAMPLE 3 GEP Antagonizes TNFq Action 1001791 Atsttrin antagonizes TNFz-induced inflammation response: We next determined whether Atsttrin inhibits TNF-mediated inflammation. Neutrophils are triggered by inflammatory stimuli like TNFq to generate large quantities of reactive oxygen species that contribute to neutrophil activation and the development of inflammatory processes [59]. Accordingly, we tested the effect of Atsttrin on TNFco-induced neutrophil activation. As shown in FIGURE 16A, Atsttrin dose-dependently inhibits neutrophil activation triggered by TNFoR Importantly and remarkably, Atsttrin exhibits inhibition that rivals (is at least as good as) Enbrel and Remicade (FIGURE 16B), which have been used clinically for treating various kinds of inflammatory diseases, including particularly rheumatoid arthritis [60]. 50 100180] GEP inhibits TNF a-induced cell death: TNF- ahas been shown to induce apoptosis in chondrocyte cultures [61-631. We next determined whether GEP affected TNF-a-induced apoptosis in chondrocytes. Briefly, rat chondrosarcoma (RCS) cells and human C2812 chondrocytes were serum starved for 24 h to remove the effect of exogenous growth factors and cytokines. Thereafter, cells were stimulated with 0.02%BSA (CTR, control), lOOng/ml of GEP (GEP), bOng/mi of TNF-o, or GEP plus TNF-afor 36 h. Apoptosis was measured by using an TUNEL assay kit (Promega). As shown in FIGURE 17, TNF-ainduced prominent cell death in both RCS and C2812 chondrocytes, whereas GEP dramatically inhibited TNF--a induction of apoptosis in chondrocytes. 1001811 GEP inhibits TNFt-induced metalloproteinase: We have recently reported that TNF a induces expression of ADAMTS-7 and ADAMTS- 12, two metalloproteinases in the ADAMTS family [64]. We next determined whether GEP inhibited induction of ADAMTS by TNFcX Briefly, human C2812 chondrocytes or cartilage explants were treated with TNFq in the absence or presence of various amount of GEP for 48 hours, and the expression of ADAMTS-7 and ADAMTS- 12 were determined using real, time PCR with their specific primers. In accordance with our previously report results[64], TNFq clearly induced ADAMTS-7 and ADAMTS- 12 expression (FIGURE 18). GEP demonstrated a dose-dependent inhibition of TNF c-mediated induction of metalloproteinase in chondrocytes or cartilage explants (FIGURE 18). 100182] Brief Summary of Major Characteristics of GEP and Atsttrin: Compared to the currently available anti-inflammation engineered antibodies or recombinant protein blockers, including etanercept (Enbrel, a soluble TNFR2-IgG1 fusion protein), infliximab (Remicade, a chimeric monoclonal antibody against TNF-0), and adalimumab (a humaneric monoclonal antibody against TNF- a), GEP and its derived Atsttrin have the features as below: 100183] i) Unique anti-TNF/TNFR property: Antibodies and immunoadhesins that directly target cytokines for their systemic removal (ligand ablation) have become an effective therapeutic strategy (e.g. etanercept, adalimumab and infliximab), and in some indications the selective targeting of cytokine receptors (e.g. anakinra) can deliver a more highly effective clinical outcome. Our studies demonstrates that GEP is the first known growth factor that directly targets to TNF receptors (TNFR), thus GEP and its derived peptide(s) represent the first anti-TNF/TNFR signaling blockers through acting 51 on the cytokine receptors, resembling the action of Anakinra that targets to IL-I receptor. (See FIGURE 19 for a comparison of the distinct anti-inflammation mechanisms between GEP/Atsttrin and Enbrel/Remicade). Due to its unique anti-TNF/TNFR signaling activity, Atsttrin may also work well for the patient population who does not respond to current TNFablockers, such as Remicade. [00184] ii) Low toxicity: Since Atsttrin is derived a naturally-occurring GEP factor that is already present in body fluids, it is expected that GEP and Atsttrin will not cause immunologic issues or responses and will exhibit no or less toxicity when compared to other engineered recombinant proteins. 1001851 iii) Multiple Functions: In addition to its anti-inflammatory activity, GEP also has potent tissue-repair function, thus GEP and Atsttrin are expected to block inflammation reaction on one hand, and also repair the injured tissues by inflammation response. In contrast, all current anti-TNF blockers, including Enbrel and Remicade, do not have tissue-repair activity. Furthermore, our pilot studies indicated that Atsttrin has tumor-suppression activity, which represents another application of Atsttrin. EXAMPLE 4 Atsttrin Inhibits GEP-Stimulated Cancer Cell Proliferation 100186] High levels of GEP expression are found in several human cancers and contribute to tumnorigenesis in diverse cancers, including breast cancer, clear cell renal carcinoma, invasive ovarian carcinoma, glioblastoma, adipocytic teratoma, and multiple myeloma [16, 18-24). Atsttrin inhibits TNF-mediated inflammation via blocking the binding of TNF to TNFR, and it is expected that Atsttrin may also inhibit tumor cell growth via blocking the binding of GEP to TNFR. To test this hypothesis we examined the effects of Atsttrin on GEP-stimulated cell proliferation of cancer cells. As revealed in FIGURE 20, Atsttrin dose-dependently inhibited GEP-stimulated cell proliferation of cancer cells tested. References 52 1. Martel-Pelletier, J., Pathophysiology tf osteoarthritis. Osteoarthritis Cartilage, 1999. 7(4): p. 371-3. 2. Petersson, I.F., et al., Changes in cartilage and bone metabolism identified by serum markers in early osteoarthritis cf the kneejoint. Br J Rheumatol, 1998. 37(1): p. 46-50. 3. Ayral, X., Diagnostic and quantitative arthroscopy: quantitative arthroscopy. Baillieres Clin Rheumatol, 1996. 10(3): p. 4 7 7

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2 8 . 11. Baba, T., et al., Acrogranin, an acrosomal cysteine-rich glycoprotein, is the precursor <f the growth modulatingpeptides, granulins, and epithelins, and is expressed in somatic as well as male germ cells. MoI Reprod Dev, 1993. 34(3): p. 2 3 3

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4 3 . 12. Daniel, R., et al., Cellular localization cf gene expressionfor progranulin. J Histochem Cytochem, 2000. 48(7): p. 999-1009. 13. Zanocco-Marani, T., et al., Biological activities and signalingpathways if the granulin/epithelin precursor. Cancer Res, 1999. 59(20): p. 5331-40. 14. Ong, CH. and A. Bateman, Progranulin (granulin-epithelinprecursoi, PC-cell derived growth factor, acrogranin) inproliferation and tuiorigenesis. Histol Histopathol, 2003. 18(4): p. 1275-88. 15. Hrabal, R., et al., The hairpin stackfold, a novelprotein architecturefor a newfamily cf protein growthfactors. Nat Struct Biol, 1996. 3(9): p. 747-52. 53 16. Davidson, B., et al., Granulin-epithelin precursor is a novelprognostic marker in epithelial ovarian carcinoma. Cancer, 2004. 100(10): p. 2139-47. 17. Lu, R. and G. Serrero, Inhibition f PC cell-derivedgrowlhfactor (PCDGF, epithelin/granulin precursor) expression by antisense PCDGF cDNA transfection inhibits tinorigenicity <f the human breast carcinoma cell line MDA-MB-468. Proc Natl Acad Sci U S A, 2000. 97(8): p. 3993-8. 18. Bateman, A., et al., Granulins, a novel class fpeptidefrom leukocytes. Biochem Biophys Res Commun, 1990. 173(3): p. 1161-8. 19. Gonzalez, E.M., et a\., A novel interaction between perlecan protein core andprogranuin: potential effects on tumor growth. J Biol Chem, 2003. 278(40): p. 381 13-6. 20. He, Z. and A. Bateman, Progranulin (granulin-epithelin precursor, PC-cell-derived growthfactor, acrogranin) mediates tissue repair and tumorigenesis. J Mol Med, 2003. 81(10): p. 600-12. 21. He, Z., et al., Progranulin is a mediator f the woundresponse. Nat Med, 2003. 9(2): p. 225-9. 22. Jones, M.B., M. Spooner, and E.C. Kohn, The giranulin-epithelinprecursoi: aputative new growth factorfor ovarian cancer. Gynecol Oncol, 2003. 88(1 Pt 2): p. S 136-9. 23. Wang, W., et al., PC cell-derived growthfactor (granulin precursor) expression and action in hinnan multiple myeloma. CHn Cancer Res, 2003. 9(6): p.2221-8. 24. Zhang, H. and G. Serrero, Inhibition f tumnorigenicity <f the teratoma PC cell line by transfection with antisense cDNAfor PC cell-derived growthfactor (PCDGF, epithelin/gran ulin precursor). Proc Natl Acad Sci U S A, 1998. 95(24): p. 14202-7. 25. Hoque, M., et al., Granulin and granulin repeats interact with the TaI.P-TEFb complex and inhibit Tat transactivation. J Biol Chem, 2005, 280(14): p. 13648-57. 26. Hoque, M., et al., The growthfactor granulin interacts with cyclin TI and modulates P-TEFb dependent transcription. Mol Cell Biol, 2003. 23(5): p. 1688-702. 27. Thornburg, N.J., S. Kusano, and N. Raab-Traub, Identification Cf Epstein-Barr virus RK-BARFO interactingproteins and characterization Cf expressionpattern. J Virol, 2004. 78(23): p. 12848-56. 28. Sell, C , et al., Effect cf a null mutation f the insulin-like growthfactor I receptor gene on growth and transformation f mouse embryofibroblasts. Mol Cell Biol, 1994. 14(6): p. 3604-12. 29. Xu, S.Q., et al., The granulin/epithelin precursor abrogates the requirementfor the insulin-like growthfactor 1 receptorfor growth in vitro. J Biol Chem, 1998. 273(32): p. 20078-83. 30. Sun, X., M. Gulyas, and A. Hjerpe, Mesothelial differentiation as reflected by differential gene expression. Am J Respir Cell Mol Biol, 2004, 30(4): p. 510-8. 54 3 1. Suzuki, M. and M. Nishiahara, Grandin precursor gene: a sex steroid-inducible gene involved in sexual differentiation cf the rat brain. MoI Genet Metab, 2002. 75(1): p. 31-7. 32. Barreda, D.R., et al., Differentially expressed genes that encodepotential markers <f goldfish macrophage development in vitro. Dev Comp Inmunol, 2004. 28(7-8): p. 727-46. 33. Justen, H.P., et al., Differential gene expression in synovium <f rheumatoid arthritis and osteoarthritis. MoI Cell Biol.Res Commun, 2000. 3(3): p. 165-72. 34. Zhu, J., et al., Conversion <f proepithelin to epithelins: roles <f SLPI and elastase in host defense and wound repair. Cell, 2002. 111(6): p. 867-78. 35. Baker, M., et al., Mutations inprogranulin cause tau-negativefrontotemporal dementia linked to chromosome 17. Nature, 2006. 442(7105): p. 9 1 6

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9 . 36. Cruts, M., et al., Null mutations in-progranulin cause ubiquitin-positivefrontotemporal dementia linked to chromosome 17q2L Nature, 2006. 442(7105): p. 920-4. 37. Gass, J., et aL., Mutations inprogranulin are a major cause <f ubiquitin-positivefrontotemporal lobar degeneration. Hum Mol Genet, 2006. 15(20): p. 2988-3001, 38. Rowland, L.P., Frontotemporal dementia, chromosome 17, andprogranulin. Ann Neurol, 2006. 60(3): p. 275-7. 39. Arikawa-Hirasawa, E., et al., Perlecan is essentialfor cartilage and cephalic development. Nat Genet, 1999. 23(3): p. 354-8. 40. Kvist, A.J., et al., Chondroitin sulfate perlecan enhances collagenfibrilformiation. Implicationsfor perlecan chondrodysplasias. J Biol Chem, 2006. 281(44): p. 33127-39. 4 1. Nicole, S., et al., Perlecan, the major proteoglycan if basement membranes, is altered inpatients with Schwartz-Jampel syndrome (chondrodystrophy nmyotonia). Nat Genet, 2000. 26(4): p. 480-3. 42. Wallach, D., et al., Tumor necrosisfactor receptor and Fas signaling mechanisms. Annu Rev Immunol, 1999. 17: p. 331-67. 43. Guicciardi, M.E. and G.J. Gores, AIPl: a newplayer in TNFsignaling. J CHn Invest, 2003. 111(12): p. 1813-5. 44. Gupta, S., A decision between life and death during TNF-alpha-induced signaling. J Clin Immunol, 2002. 22(4): p. 185-94. 45. Kollias, G. and D. Kontoyiannis, Role ofTNF/TNFR in autoimmunity: specific TNF receptor blockade may be advantageous to anti-TNF treatments. Cytokine Growth Factor Rev, 2002. 13(4-5): p. 315-21. 55 '46. MacRae, V.E., et al., Cytokine actions in growth disorde-s associated withpediatric chronic inflammatory diseases (review). Int I Mol Med, 2006. 18(6): p. 101 1-8. 47. MacRae, V.E., et al., Cytokine profiling and in vitro studies cf murine bone growth using biological fluids fom children withjuvenile idiopathic arthritis. Clin Endocrinol (Oxf), 2007. 67(3): p. 442-8. 48. Martensson, K., D. Chrysis, and L. Savendahl, Interleukin-Ibeta and TNF-alpha act in synergy to inhibit longitudinal growth infetal rat metatarsal bones. J Bone Miner Res, 2004. 19(1 1): p. 1805-12. 49. Xu, K., et al., Cartilage oligomeric matrix protein associates with g-anulin-epithelin precursor (GEP) andpotentiates GEP-stimulated chondrocyte proliferation. J Biol Chem, 2007. 282(15): p. 11347-55. 50. Johnson, K.A., et al., Vanin-lpantetheinase drives increased chondrogenicpotential <fq mesenchymal precursors in ank/ank mice. Am J Pathol, 2008. 172(2): p. 440-53. 51. Meirelles Lda, S. and N.B. Nardi, Murine mai-row-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization. Br J Haematol, 2003. 123(4): p. 702-1 1. 52. Longobardi, L., et al., Effect <f IGF-1 hi the chondrogenesis f bone marrow mesenchymal stein cells in the presence or absence oJTGF-beta signaling. J Bone Miner Res, 2006. 21(4): p. 626-36. 53. Attur, M.G., et aL, s' system biology" approach to bioinformatics andfunctional genomics in complex human diseases: arthritis. Curr Issues Mol Biol, 2002. 4(4): p. 129-46. 54. Yang, Y.C., et al., Hierarchical model <f gene regulation by transforming growth factor beta. Proc Natl Acad Sci U S A, 2003. 100(18): p. 10269-74. 55. Zavadil, J., et al., Genetic programs <f epithelial cellplasticity directed ly transforming growth factorbeta. Proc Natl Acad Sci U S A, 2001. 98(12): p. 6686-9 1. 56. Zavadil, J., et al., Integration <f TGF-beta/Smad and Jagged I/Notch signalling in epithelial tomesenchymal transition. Embo J, 2004. 23(5): p. 1155-65. 57. Ware, C.F., The TNFsuperfanily. Cytokine Growth Factor Rev, 2003. 14(3-4): p. 181-4. 58. Royuela, M., et al., TNF-alpha/IL-1/NF-kappaB transductionpathway in human cancer prostate. Histol Histopathol, 2008. 23(10): p. 1279-90. 59. Nathan, C.F., Neutrophil activation on biological swmfaces. Massive secretion <f hydrogen peroxide in response toproducts <f macrophages and lymphocytes. J Clin Invest, 1987. 80(6): p. 1550-60. 60. Rothe, A., B.E. Power, and P.J. Hudson, Therapeutic advances in rheumatology with the use <f recombinantproteins. Nat Clin Pract Rheumatol, 2008. 4(1 1): p. 605-14. 6 1. Aizawa, T., et al., Induction ofapoptosis in chondrocytes by tumor necrosisfactor-alpha. J Orthop Res, 2001. 19(5): p. 785-96. 56 62. Horiguchi, M., et al., Tumour necrosisfactor-alpha up-regulates the expression ofBMP-4 mRNA but inhibits chondrogenesis in mouse clonal chondrogenic EC cells, A TDC5. Cytokine, 2000. 12(5): p. 526 530. 63, MacRae, V.E., C. Farquharson, and S.F. Ahmed, Thepathophysiology cf the growth plate injuvenile idiopathic arthritis. Rheumatology (Oxford), 2006. 45(1): p. 11-9. 64. Luan, Y., et al., Inhibition cfADAMTS-7 andADAMTS- 12 degradation <f cartilage oligomeric matrix protein by alpha-2-macroglobulin. Osteoarthritis Cartilage, 2008. 16(1 1): p. 1413-20, 65. He, Z. and Bateman, A., Progranulin gene expression regulates epithelial cell growth and promotes tumorgrowth in vivo. Cancer Res, 1999. 59, p. 3222. 66. He, Z. et al., Progranulin is a mediator (f the wound response. Nat Med, 2003. 9, p. 225. 67. Zhu, J. et al., Conversion <fproepithelin to epithelins: roles cf SLPI and elastase in host defense and wound repair. Cell, 2002. 111, p. 867. EXAMPLE 5 Acute Inflammation Air Pouch Model Studies [00187] Anti-inflammatory activity of GEP and Atsttrin were tested in an air pouch-induced acute inflammation animal model. To induce air pouches, 10-15-week-old male mice were injected subcutaneously on the back with 3 ml of air. After 2 days, the pouches were reinflated with 1.5 ml of air. On day 6, inflammation was induced by injection of 1 ml of a suspension of carrageenan (2% weight/volume in calcium- and magnesium-free phosphate buffered saline solution [PBS]) into the air pouch. Remicade (IOug/g), GEP (lOug/g) and Atsttrin (lOug/g) was administered 1 hour prior to induction of inflammation in the air pouch. After 4 hours, the mice were killed by CO, narcosis, the pouches were flushed with 2 m I of PBS, and exudates were harvested. After centrifugation (1,000g for 10 minutes), the cell-free exudates were collected. The IL-6 and IL- 13 concentration was quantitated in the exudates in duplicate by enzyme-linked immunosorbent assay (R&D Systems, Minneapolis, MM) following the manufacturer's instructions. As showed in FIGURE 21, both GEP and its derived Atsttrin potently reduce the level of IL-6 and IL- 13, two major inflammatory mediators. Note that both GEP and Atsttrin exhibit more effective anti-inflammation (approximately 80% reduction in IL- 13 concentration) than Remicade in this model. 57 EXAMPLE 6 Chronic Inflammation Animal Model Studies 1001881 GEP activity is determined in a rheumatoid arthritis animal model. in this study, female BALB/cJ mice are used. The animals are housed at a density of three to four per cage and allowed to acclimate for one week. A combination of four different monoclonal antibodies to the well-defined epitopes of Collagen Ii (mAbs: Cl Ib, J1, D3, and Ul) are administered IV (on Day 0). Three days later (Day 3), mice are challenged with lipopolysaccharide administered IP (LPS, 25 .ig/mouse). Test compound and vehicle are administered according to study design beginning one hour post-LPS challenge on Day 3.Paw edema is measured on Days -1, 4, 7, 10 and 11. Arthritis Scores in mice are visually examined for signs ofjoint inflammation on Days - 1, 3, 5, 7, and 10. Body weights are determined on all days when arthritis scores are obtained. Blood is drawn at necropsy and processed to serum or plasma. Mice are euthanized after obtaining final caliper measurements on Day 11. Hind legs are taken and fixed for histopathology analysis. EXAMPLE 7 GEP and Atsttrin Effects on Progression of Arthritis in TNF a Transgenic Mice [00189] Mice transgenic for human TNFo, originally generated by Dr. George Kollias I laboratory, develop a chronic inflammatory and destructive polyarthritis with many characteristics observed in rheumatoid arthritis patients[55J. The phenotype of this mouse model validated the theory that TNF a is at the apex of the pro-inflammatory cascade in rheumatoid arthritis, and foreshadowed the remarkable success of anti-TNF a therapy that has transformed the effective management of this disease. As such, the TNFatransgenic mice are very useful tools for dissecting the molecular mechanisms of the pathogenic process and evaluating the efficacy of novel therapeutic strategies for rheumatoid arthritis [1 15, 138, 139]. This model is used to further assess GEP, especially its derived peptide(s) atsttrin, direct administration to treat rheumatoid arthritis. Briefly, TNFa transgenic mice (n = 60, purchased from Taconic) are divided into 6 groups of 10 mice each and receive GEP, GEP-derived peptide, and anti TNFa antibody (serves as a control) at doses described in the literature as being effective [140, 141]. All treatments are administered by intraperitoneal injection. Group I is treated with phosphate buffered saline and serves as a negative control. Group 2 and 3 (low does of GEP and GEP-derived peptide(s)) receive 58 lmg/g of GEP or peptide(s) 3 times weekly from week 4 to week 10. Group 4 and 5 (high does) receive lOmg/g of GEP or peptide(s) 3 times weekly from week 4 to week 10. Group 6 receive 10 mg/g of anti TNFa 3 times weekly from week 4 to week 10. At week 10, all animals are sacrificed by cervical dislocation, blood is withdrawn by heart puncture, and the paws and tibial bones are dislocated for further analyses. Clinical evaluation is performed weekly, starting at 4 weeks after birth. Arthritis is evaluated in each group of animals. EXAMPLE 8 GEP and Atsttrin Effects on RANKL-Induced Osteoclastogenesis {00190] Osteoclasts are the bone-resorbing cells, and their excess activity causes osteoporosis. RANKL is the the key receptor for osteoclastogenesis and binds RANK. RANK and TNFR belong to the same TNFR family and they share significant similarity in sequence and particularly in structure. Given that (1) RANK, the key receptor for osteoclastogenesis, belongs to the TNFR subfamily, and that (2) GEP and its derived peptide Atsttrin binds to TNFR and blocks TNF alpha action, we also examined whether GEP and Atsttrin affect osteoclastogenesis. RANKL-induced osteoclastogenesis was assessed in the presence and absence of GEP or peptide atsttrin. Briefly, we cultured Raw 264.7 macrophages in the presence of RANKL for 4 days and TRAP staining was performed. As expected, RANKL induced robust osteoclastogenesis and TRAP multinucleated positive cells were observed (FIGURE 22, indicated with arrows). GEP and atsttrin demonstrated does-dependent inhibition of osteoblast differentiation (FIGURE 22). Interesting, atsttrin appears to be more potent than GEP in blocking osteoclastogenesis. The finding that atsttrin inhibits osteoclastogenesis indicates that this peptide also has potential for treating osteoporosis in addition to various kinds of inflammatory diseases, including rheumatoid arthritis. EXAMPLE 9 GEP and Atsttrin Binding to TNF Family Members RANKL and FAS 1001911 The interactions between GEP/Atsttrin and other members in TNF receptors (TNFR) subfamily, including RANK and FAS, were examined. To assess binding, various pairs of plasmids, were co-transformed into yeast strain MAV203 and a yeast two-hybrid assay was performed (FIGURE 26). GEP plasmids were co-transformed with each of TNFR1, TNFR2, RANK and FAS plasmids. Atsttrin 59 plasmids were co-transformed with each of TNFR1, TNFR2, RANK and FAS plasmids. Yeast transformants were selected on SD-leu' /trp' /his'/uraVSAT* plates and tested for p-galactosidase activity. The lack of interaction between Rb and lamin was used as a negative control. By this two-hybrid assay, GEP associates with RANK and FAS in addition to TNFR, whereas Atsttrin specifically binds to TNFR. [001921 These studies demonstrate that GEP also binds to RANK and Fas, although the interaction may be weaker than that with TNFRI and TNFR2. hi contrast, Atsttrin, which shows higher binding affinity to TNF (TNFRl and TNFR2) receptors than does GEP, does not interact directly with RANK and FAS as assessed by two-hybrid binding assay. Thus, due to its specificity for TNFR, Atsttrin may have less or distinct side-effects and toxicity than does GEP because GEP associates with the other member of the TNFR family RANK and thus may affect multiple pathophysiological processes. 1001931 We have previously showed that Atsttrin potently inhibits RANKL-induced osteoclastogenesis (Example 8). The present example results now demonstrate that Atsttrin does not bind to RANK. Collectively, these data suggest that Atsttrin-mediated inhibition of osteoclastgenesis must be through blocking the TNF/TNFR pathway. Indeed, growing evidence demonstrates that TNF/TNFR signaling is also crucial for osteoclastogenesis. EXAMPLE i GEP Exhibits Higher Binding Affinity for TNFR Than Does TNFx [001941 Kinetic binding studies of GEP and TNFctto TNFR were observed and analyzed using Analytical Surface Plasmon Resonance with SensiQ Pioneer (ICx Nomadics, Oklahoma City, OK 73104). The kinetic binding is shown in FIGURE 27, and the kinetic constants are summarized in TABLE 1. Remarkably, GEP exhibits higher affinity for TNF receptors, especially TNFR2 when compared to TNFa (KD of GEP vs. TNFa: 1.28 x 10-9 M vs. 7.64 x 10- 7 M). In contrast to TNFa, which shows much higher affinity for TNFRI (KD 8.8 x 10" M) than TNFR2 (KD 7.64 x 10 7 M), GEP exhibits slightly higher affinity for TNFR2 (KD 1.28 x 10 9 M) than TNFR1 (KZ) 1.58 x 10- M). 60 TABLE 1 Kinetic Constants Analyte K.(x 1041S') Ka (x 1P S) KD(M) TNFa/TNFR1 8.25 7.27 8.80 X 10V GEP/TNFRI 51.6 8.14 1.58 x 10 TNFa/TNFR2 7.79 59.5 7.64 x 10" GEP/TNFR2 540 6.92 T.28 x 10_ EXAMPLE 11 Binding Studies of Atsttrin and TNFR 1001951 Atsttrin was expressed in bacteria as a GST fusion protein, purified on glutathione agarose resin, and eluted using Xa factor (there is a Xa factor cleavage site between GST and Atsttrin) (FIGURE 28A). Xa factor was then removed from the elution using Xa Removal Resin (Qiagen). Purified Atsttrin was analyzed for endotoxin using the Linulus amebocyte lysate assay, which indicated endotoxin levels similar to control medium, many fold below the manufacturer's specification of< 1 unit/gg. Using Analytical Surface Plasmon Resonance Assay (FIGURE 28 B & C), we were excited to find that Atsttrin exhibited -9-fold higher binding affinity for TNFR2 (KZ) of Atsttrin/TNFR2 vs.TNFo/TNFR2: 8.59 x 10-iM vs. 7.64 x 10 7 M), but - 18-fold lower affinity for TNFR1 than TNF a (KZ) of Atsttrin/TNFRI vs.TNF/TNFRI: 1.60 x 10M vs. 8.80 x 10 9 M), suggesting that Atsttrin can block the TNFo/TNFR2 pathway effectively, but may not significantly affect TNFQa/TNFR signaling. EXAMPLE 12 Atsttrin Fails to Activate Erkl/2 and Akt Signaling and Inhibits Cancer Cell Proliferation [001961 Using the PathScan@ Multiplex Western Cocktail I (Cell Signaling) that allows one to simultaneously detect levels of phospho-p90RSK, phospho-Akt, phospho-p44/42 MAPK (Erkl/2), and phospho-S6 ribosomal protein on a single membrane, we next sought to compare GEP- and Atsttrin activated signaling in chondrocytes. Human C2812 chondrocytes (provided by Dr. Mary B. Goldring) 61 - were starved for 24 hr and treated with 50 ng/ml of GEP or Atsttrin for various time points, and cell lysates were analyzed using the PathScan@ Multiplex Western Cocktail 1. As shown in FIGURE 29, GEP strongly activated Erkl/2 and moderately activated Akt pathways (Feng, J., Guo, F., Jiang, B., Frenkel, S., Zhang, Y., Wang, D., Liu, C.J., GEP: A BMP2-Inducible Growth Factor that Activates Erkl/2 Signaling and JunB Transcription Factor in Chondrogenesis, FASEBJ, 2010 Feb. 2 [Epub ahead of print]). However, Atsttrin, while retaining the TNFR-binding activity of GEP (FIGURE 28), loses GEP' s oncogenic signaling. When GEP and Atsttrin are tested in combination, Atsttrin blocks or suppresses GEP-mediated activation of p-AKT and p-ERK (FIGURE 29). EXAMPLE 13 Atsttrin Prevents the Onset of Arthritis in a Collagen-Induced arthritis (CIA) Model 1001971 We next examined Atsttrin in a collagen-induced arthritis (CLA) mouse model that exhibits many of the clinical and pathological features of RA. Briefly, DBA/I mice were challenged on day 0 with chick collagen I emulsified in modified complete Freund's adjuvant given s.c. at the base of the tail (Chondrex Single Immunization). On day 19, mice were divided into three groups (each n= 10) and treated every other day until day 35, as follows: Group 1 received Atsttrin at a dose of 10 pg/g body weight i.p., Group 2 received Enbrel (soluble extracellular domain of TNFR2) at a dose of 10 pg/g body weight i.p. (serving as positive control), and Group 3 received an equal volume of phosphate-buffered saline (PBS, serving as negative control). Mice were monitored daily for incidence of arthritis, arthritis severity score, and paw thickness measured by a constant pressure caliper. As shown in FIGURE 30A and 30B, both Atsttrin and Enbrel effectively prevented the development of arthritis. Atsttrin was more potent than Enbrel in this model, since Atsttrin completely prevented the onset of arthritis. As seen in the representative panels in FIGURE 31, symptoms of CIA (swelling, erythema, deformity) were apparent in mice treated with PBS. In contrast, mice treated with Atsttrin and Enbrel demonstrated markedly reduced pathology, and Atsttrin-treated mice were similar to normal mice. Ankles from CIA mice treated with PBS exhibited robust leukocyte infiltration and tissue destruction (H&E staining) and loss of matrix staining (Sarfranin-0 staining) (FIGURE 32A). Arthritic symptoms were absent in Atsttrin-treated mice. MicroCT images (FIGURE 32B) revealed clear bone erosion in CIA mice treated with PBS, but not with Atsttrin. In addition, bone-resorbing osteoclasts were clearly seen with TRAP+ around the erosive area in 62 CIA mice treated with PBS; in contrast, TRAP+ osteoclasts were hardly detectable in Atsttrin-treated CIA mice (FIGURE 33). 1001981 We evaluated Atsttrin effects of the levels of proinflammatory and anti-inflammatory cytokines in the sera of the CIA mice. Atsttrin was compared to PBS and Enbrel in these studies. Proinflammatory cytokines IL-1 I and IL-6 were evaluated and anti-inflammatory cytokines IL-10 and IL 13 were assessed. As shown in FIGURE 34, both Atsttri and Enbrel significantly reduced the levels of the pro-inflammatory cytokine IL-6. IL-I P was less significantly reduced by Enbrel and Atsttrin, although Atsttrin reduced IL-I P more than Enbrel did. With regard to anti-inflammatory cytokines, while both Enbrel and Atsttrin increased IL-IO, Atsttrin showed a more significant effect on IL-10 levels. Both Enbrel and Atsttrin increased the amount of the anti-inflammatory cytokine IL-13. EXAMPLE 14 Effects of Atsttrin on TNF-induced Activities in Cells 1001991 In vitro cell-based studies in RAW 264.7 cells were utilized to further assess and evaluate Atsttrin effects of TNF responses in cells. GEP and Atsttrin were evaluated for their effects on TNF induced nitrite production. Nitrite production was determined in cells in the presence of added TNF (500ng/ml) and with increasing amounts of either GEP, Atsttrin or Enbrel (0.3, 1.5 and 7.5 nM). While GEP reduced nitrite production somewhat, each of Atsttrin and Enrel reduced TNF-induced nitrite production more significantly (FIGURE 36). Next, TNF-induced nuclear accumulation of NFKB was evaluate in cells. Both GEP and Atsttrin blocked TNF-induced nuclear accumulation of NFKB as assessed by NFKB p 6 5 staining (FIGURE 37). Induction of a TNF-activated NFKB reporter gene (PAK-I with an NFKB binding site) was determined in the presence of TNFo and either GEP or Atattrin at increasing concentrations (FIGURE 38). Atsttrin inhibited the reporter gene's induction more significantly that GEP at lower concentrations. 1002001 This invention may be embodied in other forms or carried out in other ways without departing from the spirit or essential characteristics thereof. The present disclosure is therefore to be considered as in all aspects illustrate and not restrictive, the scope of the invention being indicated by the 63 appended Claims, and all changes which come within the meaning and range of equivalency are intended to be embraced therein. 100201] Various references are cited throughout this Specification, each of which is incorporated herein by reference in its entirety. 64

Claims (20)

1. An isolated peptide comprising one or more granulin unit and one or more linker unit of Granulin/epithelin precursor (GEP), including variants thereof, and capable of antagonizing TNF family member signaling.

2. The isolated peptide of claim 1 comprising one or one or more granulin unit and one or more linker unit of human GEP of FIGURE 23 and capable of antagonizing TNF and TNF/TNFR signaling.

3. The isolated peptide of claim I comprising at least %AF, ViK, and VAC granulin units of GEP and linker units P3, P4 and P5 of GEP as set out in FIGURE 23 and/or FIGURE 24.

4. The isolated peptide of claim 1 comprising '/2F-P3-P4-'/2A-P5-!/2C as set out in FIGURE 24 (SEQ ID NO: 2).

5. The isolated peptide of claim 1 wherein said peptide or variant is capable of binding TNFRI and/or TNFR2.

6. A composition comprising one or more peptide(s) of claim I and one or more of an anti inflammatory agent or compound, an anti-cancer agent or compound, and an immunomodulatory agent.

7. The composition of claim 6 which is a therapeutic or pharmaceutical composition, optionally further comprising a pharmaceutically acceptable carrier, vehicle, or diluent.

8. A pharmaceutical composition comprising the peptide of claim I and a pharmaceutically acceptable carrier, vehicle, or diluent.

9. An isolated nucleic acid capable of encoding a peptide of any of claims 1-5.

10. A vector comprising the nucleic acid of claim 9 and a means for expression of said nucleic acid.

11. The vector of claim 10 wherein the nucleic acid is operably linked to signals enabling expression of the nucleic acid in a cell. 65

12. A method of modulating, blocking, or inhibiting TNF or TNF/TNFR activity or signaling in a mammalian cell or subject comprising administering to said cell or subject a composition comprising GEP, a GEP peptide, or atsttrin.

13. A method of modulating, blocking, or inhibiting TNF activity or signaling in a mammalian cell or subject comprising administering to said cell or subject the composition of claim S.

14. A method of preventing, alleviating, and/or treating a disease or condition mediated by TNF/TNFR signaling in a subject comprising administering to said subject a composition comprising GEP, a GEP peptide, or atsttrin.

15. A method of preventing, alleviating, and/or treating a disease or condition mediated by TNF/TNFR signaling in a subject comprising administering to said subject the composition of claim 8.

16. The method of claim 14 or 15 wherein said disease or condition is an inflammatory disease or condition, an immunological disease or condition, or cancer.

17. The method of claim 16 wherein said inflammatory disease or condition is selected from rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, psoriasis, inflammatory bowel diseases, Crohn's disease, ulcerative colitis, uveitis, inflammatory lung diseases, and chronic obstructive pulmonary disease.

18. The method of claim 16 wherein said immunological disease or condition is selected from allergy and auto-immune disease.

19. An assay system for screening of potential drugs or compounds effective to antagonize or inhibit TNF/TNFR activity comprising administering potential drug(s) or compound(s) to a cellular sample with TNF to activate TNF/TNFR activity and determining the effect of the potential drug(s) or compound(s) upon TNF activity, by comparison with a control, wherein the control is an antagonist of TNF.

20. The assay system of claim 18 wherein said TNF is TNFot and said control is a peptide of claim 1. 2 1. The assay system of claim 18 wherein said TNF is TNFa and said control is asttrin. 66