AU2002227156A1 - Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity - Google Patents
Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelityInfo
- Publication number
- AU2002227156A1 AU2002227156A1 AU2002227156A AU2715602A AU2002227156A1 AU 2002227156 A1 AU2002227156 A1 AU 2002227156A1 AU 2002227156 A AU2002227156 A AU 2002227156A AU 2715602 A AU2715602 A AU 2715602A AU 2002227156 A1 AU2002227156 A1 AU 2002227156A1
- Authority
- AU
- Australia
- Prior art keywords
- compositions
- methods
- nucleic acid
- acid synthesis
- enzymatic nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US25076400P | 2000-12-01 | 2000-12-01 | |
US60/250,764 | 2000-12-01 | ||
PCT/US2001/045819 WO2002044425A2 (en) | 2000-12-01 | 2001-12-03 | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2002227156A1 true AU2002227156A1 (en) | 2002-06-11 |
Family
ID=22949045
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2002227156A Abandoned AU2002227156A1 (en) | 2000-12-01 | 2001-12-03 | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
Country Status (4)
Country | Link |
---|---|
US (12) | US7211414B2 (en) |
EP (1) | EP1354064A2 (en) |
AU (1) | AU2002227156A1 (en) |
WO (1) | WO2002044425A2 (en) |
Families Citing this family (454)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5823720A (en) * | 1996-02-16 | 1998-10-20 | Bitmoore | High precision cutting tools |
US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US6780591B2 (en) * | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US20040106110A1 (en) * | 1998-07-30 | 2004-06-03 | Solexa, Ltd. | Preparation of polynucleotide arrays |
US20100130368A1 (en) * | 1998-07-30 | 2010-05-27 | Shankar Balasubramanian | Method and system for sequencing polynucleotides |
US6982146B1 (en) * | 1999-08-30 | 2006-01-03 | The United States Of America As Represented By The Department Of Health And Human Services | High speed parallel molecular nucleic acid sequencing |
CN100462433C (en) * | 2000-07-07 | 2009-02-18 | 维西根生物技术公司 | Real-time sequence determination |
US20070172866A1 (en) * | 2000-07-07 | 2007-07-26 | Susan Hardin | Methods for sequence determination using depolymerizing agent |
AU2002227156A1 (en) * | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
US7668697B2 (en) * | 2006-02-06 | 2010-02-23 | Andrei Volkov | Method for analyzing dynamic detectable events at the single molecule level |
US7560254B2 (en) | 2001-08-29 | 2009-07-14 | Ge Healthcare Bio-Sciences Corp. | Allele specific primer extension |
EP1497304B1 (en) * | 2002-04-12 | 2014-06-25 | Catalyst Assets LLC | Dual-labeled nucleotides |
US20030228611A1 (en) * | 2002-05-01 | 2003-12-11 | President And Fellows Of Harvard College | Nucleic acid memory device |
AU2003263025B2 (en) * | 2002-08-29 | 2009-06-18 | Global Life Sciences Solutions Usa Llc | Analyte detection |
WO2004020604A2 (en) * | 2002-08-29 | 2004-03-11 | Amersham Biosciences Corp | Allele specific primer extension |
JP4510628B2 (en) * | 2002-08-29 | 2010-07-28 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | Terminal phosphate block nucleoside polyphosphate |
US7563600B2 (en) * | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
US20090186343A1 (en) * | 2003-01-28 | 2009-07-23 | Visigen Biotechnologies, Inc. | Methods for preparing modified biomolecules, modified biomolecules and methods for using same |
ES2355555T3 (en) * | 2003-02-05 | 2011-03-28 | Ge Healthcare Bio-Sciences Corp. | AMPLIFICATION OF NUCLEIC ACIDS. |
US7233393B2 (en) | 2004-08-05 | 2007-06-19 | Applera Corporation | Signal noise reduction for imaging in biological analysis |
US7462452B2 (en) | 2004-04-30 | 2008-12-09 | Pacific Biosciences Of California, Inc. | Field-switch sequencing |
US7482120B2 (en) * | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
AU2006213692C1 (en) * | 2005-02-09 | 2011-08-04 | Pacific Biosciences Of California, Inc. | Nucleotide compositions and uses thereof |
JP2006262829A (en) * | 2005-03-25 | 2006-10-05 | Hitachi High-Technologies Corp | Method for pre-treating biological sample, analysis system and element for pre-treating biological sample |
US9169510B2 (en) * | 2005-06-21 | 2015-10-27 | The Trustees Of Columbia University In The City Of New York | Pyrosequencing methods and related compositions |
US8822670B2 (en) * | 2005-08-19 | 2014-09-02 | Sumitomo Bakelite Company, Ltd. | Method of synthesizing cDNA and method of synthesizing RNA chains, and nucleotide-immobilized carrier |
US20070190542A1 (en) * | 2005-10-03 | 2007-08-16 | Ling Xinsheng S | Hybridization assisted nanopore sequencing |
JP2009519041A (en) * | 2005-12-12 | 2009-05-14 | アメリカ合衆国 | Probes and methods of use for nucleic acid sequencing |
US8703734B2 (en) | 2005-12-12 | 2014-04-22 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Nanoprobes for detection or modification of molecules |
US20080241938A1 (en) * | 2006-07-20 | 2008-10-02 | Visigen Biotechnologies, Inc. | Automated synthesis or sequencing apparatus and method for making and using same |
US20080241951A1 (en) * | 2006-07-20 | 2008-10-02 | Visigen Biotechnologies, Inc. | Method and apparatus for moving stage detection of single molecular events |
US20080091005A1 (en) * | 2006-07-20 | 2008-04-17 | Visigen Biotechnologies, Inc. | Modified nucleotides, methods for making and using same |
WO2008027558A2 (en) | 2006-08-31 | 2008-03-06 | Codon Devices, Inc. | Iterative nucleic acid assembly using activation of vector-encoded traits |
US20080108804A1 (en) * | 2006-11-02 | 2008-05-08 | Kabushiki Kaisha Dnaform | Method for modifying RNAS and preparing DNAS from RNAS |
WO2008151023A2 (en) | 2007-06-01 | 2008-12-11 | Ibis Biosciences, Inc. | Methods and compositions for multiple displacement amplification of nucleic acids |
WO2009046094A1 (en) | 2007-10-01 | 2009-04-09 | Nabsys, Inc. | Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment |
CN101889074A (en) * | 2007-10-04 | 2010-11-17 | 哈尔西恩莫尔丘勒公司 | Sequencing nucleic acid polymers with electron microscopy |
AU2009229157B2 (en) | 2008-03-28 | 2015-01-29 | Pacific Biosciences Of California, Inc. | Compositions and methods for nucleic acid sequencing |
EP2682460B1 (en) | 2008-07-07 | 2017-04-26 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
US9650668B2 (en) | 2008-09-03 | 2017-05-16 | Nabsys 2.0 Llc | Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels |
US8262879B2 (en) | 2008-09-03 | 2012-09-11 | Nabsys, Inc. | Devices and methods for determining the length of biopolymers and distances between probes bound thereto |
EP2342362B1 (en) * | 2008-09-03 | 2017-03-01 | Nabsys 2.0 LLC | Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels |
US8383345B2 (en) | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
WO2010062775A2 (en) | 2008-11-03 | 2010-06-03 | The Regents Of The University Of California | Methods for detecting modification resistant nucleic acids |
US8236532B2 (en) | 2008-12-23 | 2012-08-07 | Illumina, Inc. | Multibase delivery for long reads in sequencing by synthesis protocols |
WO2010111690A2 (en) | 2009-03-27 | 2010-09-30 | Life Technologies Corporation | Polymerase compositions and methods |
US20100243449A1 (en) * | 2009-03-27 | 2010-09-30 | Oliver John S | Devices and methods for analyzing biomolecules and probes bound thereto |
US8455260B2 (en) * | 2009-03-27 | 2013-06-04 | Massachusetts Institute Of Technology | Tagged-fragment map assembly |
US20100279882A1 (en) * | 2009-05-01 | 2010-11-04 | Mostafa Ronaghi | Sequencing methods |
US8246799B2 (en) * | 2009-05-28 | 2012-08-21 | Nabsys, Inc. | Devices and methods for analyzing biomolecules and probes bound thereto |
WO2011047307A1 (en) | 2009-10-15 | 2011-04-21 | Ibis Biosciences, Inc. | Multiple displacement amplification |
WO2011056872A2 (en) | 2009-11-03 | 2011-05-12 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
WO2011066185A1 (en) | 2009-11-25 | 2011-06-03 | Gen9, Inc. | Microfluidic devices and methods for gene synthesis |
US9217144B2 (en) | 2010-01-07 | 2015-12-22 | Gen9, Inc. | Assembly of high fidelity polynucleotides |
EP3151052A1 (en) | 2010-02-01 | 2017-04-05 | Illumina, Inc. | Focusing methods and optical systems and assemblies using the same |
DE202011003570U1 (en) | 2010-03-06 | 2012-01-30 | Illumina, Inc. | Systems and apparatus for detecting optical signals from a sample |
US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
CN102834526B (en) | 2010-04-05 | 2015-12-02 | 普罗格诺西斯生物科学公司 | The biological characteristis of spatial encoding |
US8715933B2 (en) | 2010-09-27 | 2014-05-06 | Nabsys, Inc. | Assay methods using nicking endonucleases |
WO2012055929A1 (en) | 2010-10-26 | 2012-05-03 | Illumina, Inc. | Sequencing methods |
US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
WO2012061832A1 (en) | 2010-11-05 | 2012-05-10 | Illumina, Inc. | Linking sequence reads using paired code tags |
EP4039363A1 (en) | 2010-11-12 | 2022-08-10 | Gen9, Inc. | Protein arrays and methods of using and making the same |
LT3360963T (en) | 2010-11-12 | 2020-02-25 | Gen9, Inc. | Methods and devices for nucleic acids synthesis |
WO2012067911A1 (en) | 2010-11-16 | 2012-05-24 | Nabsys, Inc. | Methods for sequencing a biomolecule by detecting relative positions of hybridized probes |
WO2012074855A2 (en) | 2010-11-22 | 2012-06-07 | The Regents Of The University Of California | Methods of identifying a cellular nascent rna transcript |
CN104694628B (en) * | 2010-12-13 | 2017-06-06 | 生命技术公司 | Using the activated polymerization nucleic acid reacted by polyphosphoric acid decomposition (APP) |
EP2652153B1 (en) * | 2010-12-17 | 2017-07-05 | The Trustees of Columbia University in the City of New York | Dna sequencing by synthesis using modified nucleotides and nanopore detection |
US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
WO2012103442A2 (en) | 2011-01-28 | 2012-08-02 | The Broad Institute, Inc. | Paired end bead amplification and high throughput sequencing |
EP2670864B1 (en) | 2011-01-31 | 2017-03-08 | Illumina, Inc. | Methods for reducing nucleic acid damage |
JP6017458B2 (en) | 2011-02-02 | 2016-11-02 | ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション | Mass parallel continuity mapping |
WO2012109574A2 (en) | 2011-02-11 | 2012-08-16 | Nabsys, Inc. | Assay methods using dna binding proteins |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
EP2718465B1 (en) | 2011-06-09 | 2022-04-13 | Illumina, Inc. | Method of making an analyte array |
US10364464B2 (en) | 2011-08-08 | 2019-07-30 | The Broad Institute, Inc. | Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence |
BR112014003911A2 (en) | 2011-08-19 | 2017-03-14 | Synthetic Genomics Inc | integrated method for the identification of high yield of new pesticide compositions and their use |
LT2944693T (en) | 2011-08-26 | 2019-08-26 | Gen9, Inc. | Compositions and methods for high fidelity assembly of nucleic acids |
AU2012316218B2 (en) | 2011-09-26 | 2016-03-17 | Gen-Probe Incorporated | Algorithms for sequence determinations |
WO2013063382A2 (en) | 2011-10-28 | 2013-05-02 | Illumina, Inc. | Microarray fabrication system and method |
WO2013070627A2 (en) | 2011-11-07 | 2013-05-16 | Illumina, Inc. | Integrated sequencing apparatuses and methods of use |
US9200274B2 (en) | 2011-12-09 | 2015-12-01 | Illumina, Inc. | Expanded radix for polymeric tags |
NO2694769T3 (en) | 2012-03-06 | 2018-03-03 | ||
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
US20130261984A1 (en) | 2012-03-30 | 2013-10-03 | Illumina, Inc. | Methods and systems for determining fetal chromosomal abnormalities |
ES2949570T3 (en) | 2012-04-03 | 2023-09-29 | Illumina Inc | Integrated optoelectronic readout head and fluid cartridge useful for nucleic acid sequencing |
US20130274148A1 (en) | 2012-04-11 | 2013-10-17 | Illumina, Inc. | Portable genetic detection and analysis system and method |
AU2013251701A1 (en) | 2012-04-24 | 2014-10-30 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
US9012022B2 (en) | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
US8895249B2 (en) | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
CA2877823A1 (en) | 2012-06-25 | 2014-01-03 | Gen9, Inc. | Methods for nucleic acid assembly and high throughput sequencing |
CA2878291A1 (en) | 2012-07-03 | 2014-01-09 | Sloan Kettering Institute For Cancer Research | Quantitative assessment of human t-cell repertoire recovery after allogeneic hematopoietic stem cell transplantation |
NL2017959B1 (en) | 2016-12-08 | 2018-06-19 | Illumina Inc | Cartridge assembly |
EP2885626B1 (en) | 2012-08-20 | 2020-03-04 | Illumina, Inc. | An integrated detection, flow cell and photonics (dfp) device |
WO2014037047A1 (en) * | 2012-09-07 | 2014-03-13 | Nokia Siemens Networks Oy | Usage control for subscriber group |
US9637782B2 (en) | 2012-09-28 | 2017-05-02 | Pacific Biosciences Of California, Inc. | Charged triplet-state quenchers for mitigation of photo-induced damage |
CA2886974C (en) | 2012-10-17 | 2021-06-29 | Spatial Transcriptomics Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
US9181583B2 (en) | 2012-10-23 | 2015-11-10 | Illumina, Inc. | HLA typing using selective amplification and sequencing |
US9914966B1 (en) | 2012-12-20 | 2018-03-13 | Nabsys 2.0 Llc | Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation |
US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
EP2956550B1 (en) | 2013-01-18 | 2020-04-08 | Nabsys 2.0 LLC | Enhanced probe binding |
US9805407B2 (en) | 2013-01-25 | 2017-10-31 | Illumina, Inc. | Methods and systems for using a cloud computing environment to configure and sell a biological sample preparation cartridge and share related data |
US9512422B2 (en) | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
EP3553175B1 (en) | 2013-03-13 | 2021-07-14 | Illumina, Inc. | Method of preparing a nucleic acid sequencing library |
US20160023208A1 (en) | 2013-03-13 | 2016-01-28 | Illumina, Inc. | Multilayer fluidic devices and methods for their fabrication |
AU2013382024B2 (en) | 2013-03-14 | 2019-01-31 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
US9193998B2 (en) | 2013-03-15 | 2015-11-24 | Illumina, Inc. | Super resolution imaging |
US20140274747A1 (en) | 2013-03-15 | 2014-09-18 | Illumina, Inc. | Super resolution imaging |
EP2970356B1 (en) | 2013-03-15 | 2018-05-30 | Illumina Cambridge Limited | Modified nucleosides or nucleotides |
EP3013984B1 (en) | 2013-06-25 | 2023-03-22 | Prognosys Biosciences, Inc. | Methods for determining spatial patterns of biological targets in a sample |
WO2015002813A1 (en) | 2013-07-01 | 2015-01-08 | Illumina, Inc. | Catalyst-free surface functionalization and polymer grafting |
ES2719579T3 (en) | 2013-07-03 | 2019-07-11 | Illumina Inc | System for sequencing by orthogonal synthesis |
CA3091557C (en) | 2013-08-08 | 2022-10-18 | Illumina, Inc. | Fluidic system for reagent delivery to a flow cell |
AU2014312043A1 (en) | 2013-08-30 | 2016-02-25 | Illumina France | Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces |
US10540783B2 (en) | 2013-11-01 | 2020-01-21 | Illumina, Inc. | Image analysis useful for patterned objects |
US9546398B2 (en) | 2013-11-14 | 2017-01-17 | Agilent Technologies, Inc. | Polymerase idling method for single molecule DNA sequencing |
RS60736B1 (en) | 2013-12-03 | 2020-09-30 | Illumina Inc | Methods and systems for analyzing image data |
AU2014364006B2 (en) | 2013-12-10 | 2019-07-11 | Illumina, Inc. | Biosensors for biological or chemical analysis and methods of manufacturing the same |
DK3083994T3 (en) | 2013-12-20 | 2021-09-13 | Illumina Inc | Preservation of genomic connectivity information in fragmented genomic DNA samples |
JP7082860B2 (en) | 2013-12-23 | 2022-06-09 | イラミーナ インコーポレーテッド | Methods for Structured and Structured Substrate to Improve Detection of Light Radiation |
US9677132B2 (en) | 2014-01-16 | 2017-06-13 | Illumina, Inc. | Polynucleotide modification on solid support |
BR112016016546B1 (en) | 2014-01-16 | 2022-09-06 | Illumina, Inc | AMPLICON PREPARATION METHOD AND METHODS FOR DETERMINING THE PRESENCE OF A CANCER-ASSOCIATED GENE AND A TRUE NUCLEIC ACID SEQUENCE VARIANT |
BR112016019267B8 (en) | 2014-02-18 | 2023-01-10 | Illumina Inc | METHOD TO BUILD A DNA PROFILE, NUCLEIC ACID LIBRARY, METHOD TO BUILD A NUCLEIC ACID LIBRARY, PLURALITY OF PRIMERS AND KIT |
US10767219B2 (en) | 2014-03-11 | 2020-09-08 | Illumina, Inc. | Disposable, integrated microfluidic cartridge and methods of making and using same |
CA3060708A1 (en) | 2014-04-29 | 2015-11-05 | Illumina, Inc | Multiplexed single cell gene expression analysis using template switch and tagmentation |
CN106536734B (en) | 2014-05-16 | 2020-12-22 | Illumina公司 | Nucleic acid synthesis technology |
RU2682546C2 (en) | 2014-05-27 | 2019-03-19 | Иллумина, Инк. | Systems and methods of biochemical analysis including main device and removable cartridge |
CN113584139B (en) | 2014-06-03 | 2024-08-13 | 亿明达股份有限公司 | Compositions, systems and methods for detecting events using tethers anchored to or near nanoparticles |
US20150353989A1 (en) | 2014-06-09 | 2015-12-10 | Illumina Cambridge Limited | Sample preparation for nucleic acid amplification |
EP3155125A1 (en) | 2014-06-13 | 2017-04-19 | Illumina Cambridge Limited | Methods and compositions for preparing sequencing libraries |
US10017759B2 (en) | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
AU2015279794B2 (en) | 2014-06-27 | 2021-09-30 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
ES2713153T3 (en) | 2014-06-30 | 2019-05-20 | Illumina Inc | Methods and compositions that use unilateral transposition |
EP3828279B1 (en) | 2014-07-15 | 2022-11-16 | Illumina, Inc. | Biochemically activated electronic device |
CN112941065A (en) | 2014-07-21 | 2021-06-11 | 亿明达股份有限公司 | Polynucleotide enrichment Using CRISPR-CAS System |
GB201414098D0 (en) | 2014-08-08 | 2014-09-24 | Illumina Cambridge Ltd | Modified nucleotide linkers |
US9982250B2 (en) | 2014-08-21 | 2018-05-29 | Illumina Cambridge Limited | Reversible surface functionalization |
WO2016040602A1 (en) | 2014-09-11 | 2016-03-17 | Epicentre Technologies Corporation | Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv |
US10633694B2 (en) | 2014-09-12 | 2020-04-28 | Illumina, Inc. | Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence |
WO2016044233A1 (en) | 2014-09-18 | 2016-03-24 | Illumina, Inc. | Methods and systems for analyzing nucleic acid sequencing data |
CN107002051A (en) | 2014-09-30 | 2017-08-01 | 亿明达股份有限公司 | The polymerase through modification for the improved incorporation of nucleotide analog |
AU2015330688B2 (en) | 2014-10-09 | 2021-02-11 | Illumina, Inc. | Method and device for separating immiscible liquids to effectively isolate at least one of the liquids |
US9897791B2 (en) | 2014-10-16 | 2018-02-20 | Illumina, Inc. | Optical scanning systems for in situ genetic analysis |
WO2016061517A2 (en) | 2014-10-17 | 2016-04-21 | Illumina Cambridge Limited | Contiguity preserving transposition |
EP3632944B1 (en) | 2014-10-31 | 2021-12-01 | Illumina Cambridge Limited | Polymers and dna copolymer coatings |
GB201419731D0 (en) | 2014-11-05 | 2014-12-17 | Illumina Cambridge Ltd | Sequencing from multiple primers to increase data rate and density |
CN107250358B (en) | 2014-11-05 | 2021-03-30 | 伊卢米纳剑桥有限公司 | Use of siderophore chelators to reduce DNA damage during sample preparation and sequencing |
PT3218511T (en) | 2014-11-11 | 2020-07-23 | Illumina Cambridge Ltd | Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid |
EP3218513B1 (en) | 2014-11-11 | 2018-10-31 | Illumina, Inc. | Polynucleotide amplification using crispr-cas systems |
ES2870097T3 (en) | 2014-12-15 | 2021-10-26 | Illumina Inc | Single molecular placement method on a substrate |
CA2975739C (en) | 2015-02-10 | 2022-12-06 | Illumina, Inc. | Methods and compositions for analyzing cellular components |
WO2016154038A1 (en) | 2015-03-20 | 2016-09-29 | Illumina, Inc. | Fluidics cartridge for use in the vertical or substantially vertical position |
EP3274692B1 (en) | 2015-03-24 | 2022-08-10 | Illumina, Inc. | Methods for imaging samples for biological or chemical analysis |
WO2016156845A1 (en) | 2015-03-31 | 2016-10-06 | Illumina Cambridge Limited | Surface concatamerization of templates |
ES2972835T3 (en) | 2015-04-10 | 2024-06-17 | 10X Genomics Sweden Ab | Multiplex analysis of biological specimens of spatially distinguished nucleic acids |
EP3283870B1 (en) | 2015-04-14 | 2020-05-06 | Illumina, Inc. | Structured substrates for improving detection of light emissions and methods relating to the same |
US10844428B2 (en) | 2015-04-28 | 2020-11-24 | Illumina, Inc. | Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS) |
CA2983932C (en) | 2015-05-11 | 2023-07-25 | Illumina, Inc. | Platform for discovery and analysis of therapeutic agents |
BR112017025587B1 (en) | 2015-05-29 | 2022-09-27 | Illumina, Inc | SAMPLE CARRIER AND ASSAY SYSTEM TO CONDUCT DESIGNATED REACTIONS |
EP3303584B1 (en) | 2015-05-29 | 2019-10-09 | Epicentre Technologies Corporation | Methods of analyzing nucleic acids |
EP3303614B1 (en) | 2015-05-29 | 2020-03-11 | Illumina Cambridge Limited | Enhanced utilization of surface primers in clusters |
IL255758B (en) | 2015-06-03 | 2022-07-01 | Illumina Inc | Compositions, systems, and methods for sequencing polynucleotides using tethers anchored to polymerases adjacent to nanopores |
EP3878974A1 (en) | 2015-07-06 | 2021-09-15 | Illumina Cambridge Limited | Sample preparation for nucleic acid amplification |
WO2017007757A1 (en) | 2015-07-06 | 2017-01-12 | Illumina, Inc. | Balanced ac modulation for driving droplet operations electrodes |
CN107924121B (en) | 2015-07-07 | 2021-06-08 | 亿明达股份有限公司 | Selective surface patterning via nanoimprinting |
CN107835857A (en) | 2015-07-17 | 2018-03-23 | 亿明达股份有限公司 | For the polymer sheet layer of application to be sequenced |
CA3242290A1 (en) | 2015-07-27 | 2017-02-02 | Illumina, Inc. | Spatial mapping of nucleic acid sequence information |
IL255445B (en) | 2015-07-30 | 2022-07-01 | Illumina Inc | Orthogonal deblocking of nucleotides |
DK3334839T3 (en) | 2015-08-14 | 2021-04-26 | Illumina Inc | SYSTEMS AND PROCEDURES USING MAGNETIC REACTIVE SENSORS FOR DETERMINING A GENETIC PROPERTY |
WO2017034868A1 (en) | 2015-08-24 | 2017-03-02 | Illumina, Inc. | In-line pressure accumulator and flow-control system for biological or chemical assays |
CN115928221A (en) | 2015-08-28 | 2023-04-07 | Illumina公司 | Single cell nucleic acid sequence analysis |
WO2017037078A1 (en) | 2015-09-02 | 2017-03-09 | Illumina Cambridge Limited | Systems and methods of improving droplet operations in fluidic systems |
US10450598B2 (en) | 2015-09-11 | 2019-10-22 | Illumina, Inc. | Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest |
SG11201803293SA (en) | 2015-10-22 | 2018-05-30 | Illumina Inc | Filler fluid for fluidic devices |
WO2017095917A1 (en) | 2015-12-01 | 2017-06-08 | Illumina, Inc. | Digital microfluidic system for single-cell isolation and characterization of analytes |
CN115881230A (en) | 2015-12-17 | 2023-03-31 | 伊路敏纳公司 | Differentiating methylation levels in complex biological samples |
JP2019508669A (en) | 2016-01-11 | 2019-03-28 | イラミーナ インコーポレーテッド | Microfluorometer, fluid system, and detection device having a flow cell latch clamp module |
CN110702652A (en) | 2016-03-24 | 2020-01-17 | 伊鲁米那股份有限公司 | Apparatus and compositions for use in luminescence imaging and methods of use thereof |
EP3831484B1 (en) | 2016-03-28 | 2024-05-01 | Illumina, Inc. | Multi-plane microarrays |
US11384391B2 (en) | 2016-04-04 | 2022-07-12 | The Trustees Of Columbia University In The City Of New York | Flourescene energy transfer-based single molecule/ensemble DNA sequencing by synthesis |
ES2786974T3 (en) | 2016-04-07 | 2020-10-14 | Illumina Inc | Methods and systems for the construction of standard nucleic acid libraries |
WO2017177017A1 (en) | 2016-04-07 | 2017-10-12 | Omniome, Inc. | Methods of quantifying target nucleic acids and identifying sequence variants |
KR102356022B1 (en) | 2016-04-22 | 2022-01-27 | 일루미나, 인코포레이티드 | Photonic structures-based devices and compositions for use in luminescent imaging of multiple sites within a pixel, and methods of using the same |
EP4269611A3 (en) | 2016-05-11 | 2024-01-17 | Illumina, Inc. | Polynucleotide enrichment and amplification using argonaute systems |
AU2017267653B2 (en) | 2016-05-18 | 2021-05-13 | Illumina, Inc. | Self assembled patterning using patterned Hydrophobic surfaces |
AU2017299803B2 (en) | 2016-07-22 | 2023-06-29 | Illumina, Inc. | Single cell whole genome libraries and combinatorial indexing methods of making thereof |
WO2018064116A1 (en) | 2016-09-28 | 2018-04-05 | Illumina, Inc. | Methods and systems for data compression |
CN111781139B (en) | 2016-10-14 | 2023-09-12 | 亿明达股份有限公司 | Clamping box assembly |
PL3529380T3 (en) | 2016-10-19 | 2022-10-24 | Illumina, Inc. | Methods for chemical ligation of nucleic acids |
JP7113838B2 (en) | 2016-11-16 | 2022-08-05 | イルミナ インコーポレイテッド | Enabling method and system for array variant calling |
GB201619458D0 (en) | 2016-11-17 | 2017-01-04 | Spatial Transcriptomics Ab | Method for spatial tagging and analysing nucleic acids in a biological specimen |
EP4119663A1 (en) | 2016-12-09 | 2023-01-18 | The Broad Institute, Inc. | Crispr effector system based diagnostics |
JP7051869B2 (en) | 2016-12-22 | 2022-04-11 | イラミーナ インコーポレーテッド | Array containing sequencing primers and non-sequencing entities |
CN110248725B (en) | 2016-12-22 | 2022-08-02 | 伊鲁米那股份有限公司 | Array comprising resin film and patterned polymer layer |
KR102624409B1 (en) | 2016-12-22 | 2024-01-11 | 일루미나, 인코포레이티드 | Arrays with quality control tracers |
EP3562962B1 (en) | 2016-12-30 | 2022-01-05 | Omniome, Inc. | Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides |
GB201704754D0 (en) | 2017-01-05 | 2017-05-10 | Illumina Inc | Kinetic exclusion amplification of nucleic acid libraries |
CA3049142A1 (en) | 2017-01-06 | 2018-07-12 | Illumina, Inc. | Phasing correction |
AU2018208462B2 (en) | 2017-01-10 | 2021-07-29 | Pacific Biosciences Of California, Inc. | Polymerases engineered to reduce nucleotide-independent DNA binding |
JP6806909B2 (en) | 2017-01-17 | 2021-01-06 | イルミナ インコーポレイテッド | Determining tumorigenic splicing variants |
SG11201906428SA (en) | 2017-01-18 | 2019-08-27 | Illumina Inc | Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths |
CN110177884B (en) | 2017-01-20 | 2021-08-06 | 欧姆尼欧美公司 | Genotyping by polymerase binding |
EP3571319A1 (en) | 2017-01-20 | 2019-11-27 | Omniome, Inc. | Process for cognate nucleotide detection in a nucleic acid sequencing workflow |
EP3571313B1 (en) | 2017-01-20 | 2020-12-23 | Omniome, Inc. | Allele-specific capture of nucleic acids |
GB201701689D0 (en) | 2017-02-01 | 2017-03-15 | Illumia Inc | System and method with fiducials of non-closed shapes |
GB201701686D0 (en) | 2017-02-01 | 2017-03-15 | Illunina Inc | System & method with fiducials having offset layouts |
GB201701688D0 (en) | 2017-02-01 | 2017-03-15 | Illumia Inc | System and method with fiducials in non-recliner layouts |
WO2018152162A1 (en) | 2017-02-15 | 2018-08-23 | Omniome, Inc. | Distinguishing sequences by detecting polymerase dissociation |
AU2018225503B2 (en) | 2017-02-21 | 2024-07-18 | Illumina Cambridge Limited | Tagmentation using immobilized transposomes with linkers |
US11104937B2 (en) | 2017-03-15 | 2021-08-31 | The Broad Institute, Inc. | CRISPR effector system based diagnostics |
US11174515B2 (en) | 2017-03-15 | 2021-11-16 | The Broad Institute, Inc. | CRISPR effector system based diagnostics |
KR20190140918A (en) | 2017-03-15 | 2019-12-20 | 더 브로드 인스티튜트, 인코퍼레이티드 | CRISPR effector system-based diagnostics for virus detection |
US11021740B2 (en) | 2017-03-15 | 2021-06-01 | The Broad Institute, Inc. | Devices for CRISPR effector system based diagnostics |
KR102548274B1 (en) | 2017-03-20 | 2023-06-27 | 일루미나, 인코포레이티드 | Methods and compositions for preparing nucleic acid libraries |
US10737267B2 (en) | 2017-04-04 | 2020-08-11 | Omniome, Inc. | Fluidic apparatus and methods useful for chemical and biological reactions |
EP3842545B1 (en) | 2017-04-23 | 2022-11-30 | Illumina, Inc. | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
CN110785492B (en) | 2017-04-23 | 2024-04-30 | 伊鲁米纳剑桥有限公司 | Compositions and methods for improved sample identification in indexed nucleic acid libraries |
CN111094584A (en) | 2017-04-23 | 2020-05-01 | 伊鲁米那股份有限公司 | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
US10161003B2 (en) | 2017-04-25 | 2018-12-25 | Omniome, Inc. | Methods and apparatus that increase sequencing-by-binding efficiency |
EP3619326A1 (en) | 2017-05-01 | 2020-03-11 | Illumina, Inc. | Optimal index sequences for multiplex massively parallel sequencing |
CN110832087A (en) | 2017-05-08 | 2020-02-21 | 伊鲁米那股份有限公司 | Universal short adaptors for indexing of polynucleotide samples |
FI3981884T3 (en) | 2017-06-07 | 2023-09-21 | Univ Oregon Health & Science | Single cell whole genome libraries for methylation sequencing |
CN111032882A (en) | 2017-06-20 | 2020-04-17 | 伊鲁米那股份有限公司 | Methods and compositions for addressing inefficiencies in amplification reactions |
AU2018302034B2 (en) | 2017-07-18 | 2021-05-20 | Pacific Biosciences Of California, Inc. | Method of chemically modifying plastic surfaces |
WO2019027767A1 (en) | 2017-07-31 | 2019-02-07 | Illumina Inc. | Sequencing system with multiplexed biological sample aggregation |
WO2019023924A1 (en) | 2017-08-01 | 2019-02-07 | Helitec Limited | Methods of enriching and determining target nucleotide sequences |
US20220411859A1 (en) | 2017-08-01 | 2022-12-29 | Illumina, Inc. | Hydrogel beads for nucleotide sequencing |
WO2019028047A1 (en) | 2017-08-01 | 2019-02-07 | Illumina, Inc | Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells |
CN114563419A (en) | 2017-08-15 | 2022-05-31 | 加利福尼亚太平洋生物科学股份有限公司 | Scanning device and method for detecting chemical and biological analytes |
US11447818B2 (en) | 2017-09-15 | 2022-09-20 | Illumina, Inc. | Universal short adapters with variable length non-random unique molecular identifiers |
CN110870020A (en) | 2017-10-16 | 2020-03-06 | 因美纳有限公司 | Aberrant splicing detection using Convolutional Neural Network (CNNS) |
KR102416048B1 (en) | 2017-10-16 | 2022-07-04 | 일루미나, 인코포레이티드 | Deep convolutional neural networks for variant classification |
WO2019136376A1 (en) | 2018-01-08 | 2019-07-11 | Illumina, Inc. | High-throughput sequencing with semiconductor-based detection |
KR102494589B1 (en) | 2018-01-08 | 2023-02-07 | 일루미나, 인코포레이티드 | High-throughput sequencing with semiconductor-based detection |
EP3901833A1 (en) | 2018-01-15 | 2021-10-27 | Illumina, Inc. | Deep learning-based variant classifier |
WO2019148206A1 (en) | 2018-01-29 | 2019-08-01 | The Broad Institute, Inc. | Crispr effector system based diagnostics |
EP3749777A4 (en) | 2018-02-06 | 2021-11-24 | Omniome, Inc. | Compositions and techniques for nucleic acid primer extension |
KR102437660B1 (en) | 2018-02-13 | 2022-08-29 | 일루미나, 인코포레이티드 | DNA sequencing using hydrogel beads |
JP6974504B2 (en) | 2018-04-02 | 2021-12-01 | イルミナ インコーポレイテッド | Compositions and Methods for Making Controls for Sequence-Based Genetic Testing |
US20190318806A1 (en) | 2018-04-12 | 2019-10-17 | Illumina, Inc. | Variant Classifier Based on Deep Neural Networks |
WO2019203986A1 (en) | 2018-04-19 | 2019-10-24 | Omniome, Inc. | Improving accuracy of base calls in nucleic acid sequencing methods |
KR102481869B1 (en) | 2018-04-20 | 2022-12-27 | 일루미나, 인코포레이티드 | Methods of encapsulating single cells, the encapsulated cells and uses thereof |
AU2019261218A1 (en) | 2018-04-26 | 2020-11-26 | Pacific Biosciences Of California, Inc. | Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes |
CN111065645A (en) | 2018-05-15 | 2020-04-24 | 伊鲁米纳公司 | Compositions and methods for chemical cleavage and deprotection of surface-bound oligonucleotides |
KR102447811B1 (en) | 2018-05-17 | 2022-09-27 | 일루미나, 인코포레이티드 | High-throughput single-cell sequencing with reduced amplification bias |
KR102605749B1 (en) | 2018-05-25 | 2023-11-23 | 일루미나, 인코포레이티드 | Circulating rna signatures specific to preeclamsia |
JP2021525078A (en) | 2018-05-31 | 2021-09-24 | オムニオム インコーポレイテッドOmniome, Inc. | Increased signal vs. noise in nucleic acid sequencing |
US11180794B2 (en) | 2018-05-31 | 2021-11-23 | Omniome, Inc. | Methods and compositions for capping nucleic acids |
FI3810774T3 (en) | 2018-06-04 | 2023-12-11 | Illumina Inc | Methods of making high-throughput single-cell transcriptome libraries |
US12073922B2 (en) | 2018-07-11 | 2024-08-27 | Illumina, Inc. | Deep learning-based framework for identifying sequence patterns that cause sequence-specific errors (SSEs) |
WO2020023362A1 (en) | 2018-07-24 | 2020-01-30 | Omniome, Inc. | Serial formation of ternary complex species |
CA3106035A1 (en) | 2018-08-07 | 2020-02-13 | The Broad Institute, Inc. | Cas12b enzymes and systems |
EP3837381A1 (en) | 2018-08-15 | 2021-06-23 | Illumina, Inc. | Compositions and methods for improving library enrichment |
US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
US11242512B2 (en) | 2018-09-17 | 2022-02-08 | Omniome, Inc. | Engineered polymerases for improved sequencing |
WO2020072816A1 (en) | 2018-10-03 | 2020-04-09 | The Broad Institute, Inc. | Crispr effector system based diagnostics for hemorrhagic fever detection |
SG11201911777QA (en) | 2018-10-15 | 2020-05-28 | Illumina Inc | Deep learning-based techniques for pre-training deep convolutional neural networks |
US11085036B2 (en) | 2018-10-26 | 2021-08-10 | Illumina, Inc. | Modulating polymer beads for DNA processing |
KR20210084349A (en) | 2018-10-31 | 2021-07-07 | 일루미나, 인코포레이티드 | Polymerases, compositions, and methods of use |
NL2022043B1 (en) | 2018-11-21 | 2020-06-03 | Akershus Univ Hf | Tagmentation-Associated Multiplex PCR Enrichment Sequencing |
CN118653217A (en) | 2018-11-30 | 2024-09-17 | Illumina公司 | Analysis of multiple analytes using a single assay |
GB2578528B (en) | 2018-12-04 | 2021-02-24 | Omniome Inc | Mixed-phase fluids for nucleic acid sequencing and other analytical assays |
EP3891305A1 (en) | 2018-12-05 | 2021-10-13 | Illumina Cambridge Limited | Methods and compositions for cluster generation by bridge amplification |
BR112020026655A2 (en) | 2018-12-05 | 2021-04-06 | Illumina, Inc. | POLYMERASES, COMPOSITIONS AND METHODS OF USE |
CN113767176A (en) | 2018-12-10 | 2021-12-07 | 10X基因组学有限公司 | Imaging system hardware |
GB201820300D0 (en) | 2018-12-13 | 2019-01-30 | 10X Genomics Inc | Method for spatial tagging and analysing genomic DNA in a biological specimen |
GB201820341D0 (en) | 2018-12-13 | 2019-01-30 | 10X Genomics Inc | Method for transposase-mediated spatial tagging and analysing genomic DNA in a biological specimen |
KR20210103931A (en) | 2018-12-14 | 2021-08-24 | 일루미나 케임브리지 리미티드 | Reduced pacing by unlabeled nucleotides during sequencing |
EP3899040A1 (en) | 2018-12-17 | 2021-10-27 | Illumina Cambridge Limited | Compositions for use in polyunucleotide sequencing |
WO2020126595A1 (en) | 2018-12-17 | 2020-06-25 | Illumina Cambridge Limited | Primer oligonucleotide for sequencing |
SG11202012807YA (en) | 2018-12-18 | 2021-01-28 | Illumina Cambridge Ltd | Methods and compositions for paired end sequencing using a single surface primer |
PL3899037T3 (en) | 2018-12-19 | 2024-04-08 | Illumina, Inc. | Methods for improving polynucleotide cluster clonality priority |
EP3899032A2 (en) | 2018-12-20 | 2021-10-27 | Omniome, Inc. | Temperature control for analysis of nucleic acids and other analytes |
US11293061B2 (en) | 2018-12-26 | 2022-04-05 | Illumina Cambridge Limited | Sequencing methods using nucleotides with 3′ AOM blocking group |
US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
AU2020206443A1 (en) | 2019-01-11 | 2021-04-15 | Illumina Cambridge Limited | Complex surface-bound transposome complexes |
WO2020167574A1 (en) | 2019-02-14 | 2020-08-20 | Omniome, Inc. | Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes |
WO2020172444A1 (en) | 2019-02-20 | 2020-08-27 | Omniome, Inc. | Scanning apparatus and methods for detecting chemical and biological analytes |
US20220033805A1 (en) | 2019-03-01 | 2022-02-03 | Sanjay Srivatsan | High-throughput single-nuclei and single-cell libraries and methods of making and of using |
US11210554B2 (en) | 2019-03-21 | 2021-12-28 | Illumina, Inc. | Artificial intelligence-based generation of sequencing metadata |
NL2023311B9 (en) | 2019-03-21 | 2021-03-12 | Illumina Inc | Artificial intelligence-based generation of sequencing metadata |
WO2020205296A1 (en) | 2019-03-21 | 2020-10-08 | Illumina, Inc. | Artificial intelligence-based generation of sequencing metadata |
NL2023314B1 (en) | 2019-03-21 | 2020-09-28 | Illumina Inc | Artificial intelligence-based quality scoring |
US11347965B2 (en) | 2019-03-21 | 2022-05-31 | Illumina, Inc. | Training data generation for artificial intelligence-based sequencing |
NL2023312B1 (en) | 2019-03-21 | 2020-09-28 | Illumina Inc | Artificial intelligence-based base calling |
NL2023310B1 (en) | 2019-03-21 | 2020-09-28 | Illumina Inc | Training data generation for artificial intelligence-based sequencing |
NL2023316B1 (en) | 2019-03-21 | 2020-09-28 | Illumina Inc | Artificial intelligence-based sequencing |
US11423306B2 (en) | 2019-05-16 | 2022-08-23 | Illumina, Inc. | Systems and devices for characterization and performance analysis of pixel-based sequencing |
US11593649B2 (en) | 2019-05-16 | 2023-02-28 | Illumina, Inc. | Base calling using convolutions |
EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
WO2020252186A1 (en) | 2019-06-11 | 2020-12-17 | Omniome, Inc. | Calibrated focus sensing |
CA3125305A1 (en) | 2019-07-12 | 2021-01-21 | Illumina Cambridge Limited | Nucleic acid library preparation using electrophoresis |
SG11202105836XA (en) | 2019-07-12 | 2021-07-29 | Illumina Cambridge Ltd | Compositions and methods for preparing nucleic acid sequencing libraries using crispr/cas9 immobilized on a solid support |
WO2021011803A1 (en) | 2019-07-16 | 2021-01-21 | Omniome, Inc. | Synthetic nucleic acids having non-natural structures |
US10656368B1 (en) | 2019-07-24 | 2020-05-19 | Omniome, Inc. | Method and system for biological imaging using a wide field objective lens |
US11180520B2 (en) | 2019-09-10 | 2021-11-23 | Omniome, Inc. | Reversible modifications of nucleotides |
WO2021050962A1 (en) | 2019-09-11 | 2021-03-18 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Cancer detection and classification |
EP4045683A1 (en) | 2019-10-18 | 2022-08-24 | Omniome, Inc. | Methods and compositions for capping nucleic acids |
WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
US20210139867A1 (en) | 2019-11-08 | 2021-05-13 | Omniome, Inc. | Engineered polymerases for improved sequencing by binding |
EP4055185A1 (en) | 2019-11-08 | 2022-09-14 | 10X Genomics, Inc. | Spatially-tagged analyte capture agents for analyte multiplexing |
CA3131748A1 (en) | 2019-11-22 | 2021-05-27 | Sarah E. SHULTZABERGER | Circulating rna signatures specific to preeclampsia |
DE202019106694U1 (en) | 2019-12-02 | 2020-03-19 | Omniome, Inc. | System for sequencing nucleic acids in fluid foam |
DE202019106695U1 (en) | 2019-12-02 | 2020-03-19 | Omniome, Inc. | System for sequencing nucleic acids in fluid foam |
US20210172012A1 (en) | 2019-12-04 | 2021-06-10 | Illumina, Inc. | Preparation of dna sequencing libraries for detection of dna pathogens in plasma |
MX2021011847A (en) | 2019-12-19 | 2021-11-17 | Illumina Inc | High-throughput single-cell libraries and methods of making and of using. |
US20210190770A1 (en) | 2019-12-23 | 2021-06-24 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
EP3891300B1 (en) | 2019-12-23 | 2023-03-29 | 10X Genomics, Inc. | Methods for spatial analysis using rna-templated ligation |
US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
EP4100161B1 (en) | 2020-02-04 | 2024-07-10 | Pacific Biosciences of California, Inc. | Flow cells and methods for their use |
US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
US11835462B2 (en) | 2020-02-11 | 2023-12-05 | 10X Genomics, Inc. | Methods and compositions for partitioning a biological sample |
US20210265015A1 (en) | 2020-02-20 | 2021-08-26 | Illumina, Inc. | Hardware Execution and Acceleration of Artificial Intelligence-Based Base Caller |
US20210265016A1 (en) | 2020-02-20 | 2021-08-26 | Illumina, Inc. | Data Compression for Artificial Intelligence-Based Base Calling |
US20210265018A1 (en) | 2020-02-20 | 2021-08-26 | Illumina, Inc. | Knowledge Distillation and Gradient Pruning-Based Compression of Artificial Intelligence-Based Base Caller |
AU2021224871A1 (en) | 2020-02-20 | 2022-09-08 | Illumina, Inc. | Artificial intelligence-based many-to-many base calling |
US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
US11926863B1 (en) | 2020-02-27 | 2024-03-12 | 10X Genomics, Inc. | Solid state single cell method for analyzing fixed biological cells |
CN115516104A (en) | 2020-03-03 | 2022-12-23 | 加利福尼亚太平洋生物科学股份有限公司 | Methods and compositions for sequencing double-stranded nucleic acids |
US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
US20230105642A1 (en) | 2020-03-30 | 2023-04-06 | Illumina, Inc. | Method and compositions for preparing nucleic acid libraries |
WO2021216708A1 (en) | 2020-04-22 | 2021-10-28 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
US20230183798A1 (en) | 2020-05-05 | 2023-06-15 | Pacific Biosciences Of California, Inc. | Compositions and methods for modifying polymerase-nucleic acid complexes |
US11188778B1 (en) | 2020-05-05 | 2021-11-30 | Illumina, Inc. | Equalization-based image processing and spatial crosstalk attenuator |
EP4150065A2 (en) | 2020-05-12 | 2023-03-22 | Illumina Inc | Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase |
AU2021275906A1 (en) | 2020-05-22 | 2022-12-22 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
EP4153775B1 (en) | 2020-05-22 | 2024-07-24 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
AU2021283174A1 (en) | 2020-06-02 | 2023-01-05 | 10X Genomics, Inc. | Nucleic acid library methods |
AU2021283184A1 (en) | 2020-06-02 | 2023-01-05 | 10X Genomics, Inc. | Spatial transcriptomics for antigen-receptors |
US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
EP4162074B1 (en) | 2020-06-08 | 2024-04-24 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
WO2021252617A1 (en) | 2020-06-09 | 2021-12-16 | Illumina, Inc. | Methods for increasing yield of sequencing libraries |
EP4446430A2 (en) | 2020-06-10 | 2024-10-16 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
US11935311B2 (en) | 2020-06-11 | 2024-03-19 | Nautilus Subsidiary, Inc. | Methods and systems for computational decoding of biological, chemical, and physical entities |
BR112022026056A2 (en) | 2020-06-22 | 2023-03-07 | Illumina Cambridge Ltd | NUCLEOSIDES AND NUCLEOTIDES WITH BLOCKING GROUP 3? ACETAL |
EP4172362B1 (en) | 2020-06-25 | 2024-09-18 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
KR20230035253A (en) | 2020-07-02 | 2023-03-13 | 일루미나, 인코포레이티드 | How to Calibrate Nucleic Acid Library Seeding Efficiency in a Flow Cell |
US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
CA3186976A1 (en) | 2020-07-08 | 2022-01-13 | Illumina, Inc. | Beads as transposome carriers |
CN116171330A (en) | 2020-08-06 | 2023-05-26 | Illumina公司 | RNA and DNA sequencing libraries using bead-linked transposomes |
JP2023537850A (en) | 2020-08-18 | 2023-09-06 | イルミナ インコーポレイテッド | Sequence-specific targeted transposition and selection and selection of nucleic acids |
US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
US20220067489A1 (en) | 2020-08-28 | 2022-03-03 | Illumina, Inc. | Detecting and Filtering Clusters Based on Artificial Intelligence-Predicted Base Calls |
WO2022053610A1 (en) | 2020-09-11 | 2022-03-17 | Illumina Cambridge Limited | Methods of enriching a target sequence from a sequencing library using hairpin adaptors |
US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
IL302207A (en) | 2020-10-21 | 2023-06-01 | Illumina Inc | Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput |
US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
WO2022140028A1 (en) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
WO2022155331A1 (en) | 2021-01-13 | 2022-07-21 | Pacific Biosciences Of California, Inc. | Surface structuring with colloidal assembly |
EP4284944A1 (en) | 2021-01-29 | 2023-12-06 | Illumina, Inc. | Methods, compositions and kits to improve seeding efficiency of flow cells with polynucleotides |
KR20230137394A (en) | 2021-02-04 | 2023-10-04 | 일루미나, 인코포레이티드 | Generation of long indexed-concatenated reads on transposome-bound beads |
US20240043915A1 (en) | 2021-02-13 | 2024-02-08 | The General Hospital Corporation | Methods and compositions for in situ macromolecule detection and uses thereof |
WO2022178267A2 (en) | 2021-02-19 | 2022-08-25 | 10X Genomics, Inc. | Modular assay support devices |
WO2022197752A1 (en) | 2021-03-16 | 2022-09-22 | Illumina, Inc. | Tile location and/or cycle based weight set selection for base calling |
EP4301870A1 (en) | 2021-03-18 | 2024-01-10 | 10X Genomics, Inc. | Multiplex capture of gene and protein expression from a biological sample |
CN117222747A (en) | 2021-03-22 | 2023-12-12 | 伊鲁米纳剑桥有限公司 | Methods for improving clonality of nucleic acid clusters |
KR20230161979A (en) | 2021-03-29 | 2023-11-28 | 일루미나, 인코포레이티드 | Improved library manufacturing methods |
MX2023011219A (en) | 2021-03-29 | 2023-10-02 | Illumina Inc | Compositions and methods for assessing dna damage in a library and normalizing amplicon size bias. |
MX2023010495A (en) | 2021-03-30 | 2023-09-18 | Illumina Inc | Improved methods of isothermal complementary dna and library preparation. |
CN117015603A (en) | 2021-03-31 | 2023-11-07 | Illumina公司 | Methods for preparing directed tagged sequencing libraries using transposon-based techniques with unique molecular identifiers for error correction |
CN115803816A (en) | 2021-03-31 | 2023-03-14 | 因美纳有限公司 | Artificial intelligence-based base detector with context awareness |
MX2023011659A (en) | 2021-04-02 | 2023-10-11 | Illumina Inc | Machine-learning model for detecting a bubble within a nucleotide-sample slide for sequencing. |
US20220336054A1 (en) | 2021-04-15 | 2022-10-20 | Illumina, Inc. | Deep Convolutional Neural Networks to Predict Variant Pathogenicity using Three-Dimensional (3D) Protein Structures |
US12070744B2 (en) | 2021-04-22 | 2024-08-27 | Illumina, Inc. | Valve assemblies and related systems |
EP4337789A1 (en) | 2021-05-10 | 2024-03-20 | Pacific Biosciences of California, Inc. | Dna amplification buffer replenishment during rolling circle amplification |
WO2022240764A1 (en) | 2021-05-10 | 2022-11-17 | Pacific Biosciences Of California, Inc. | Single-molecule seeding and amplification on a surface |
AU2022277632A1 (en) | 2021-05-20 | 2023-11-09 | Illumina Cambridge Limited | Compositions and methods for sequencing by synthesis |
EP4347879A1 (en) | 2021-06-03 | 2024-04-10 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
CN117677435A (en) | 2021-06-15 | 2024-03-08 | 伊鲁米纳公司 | Surface functionalization of non-aqueous gels for sequencing |
CN117597453A (en) | 2021-06-23 | 2024-02-23 | 伊鲁米纳公司 | Compositions, methods, kits, cartridges, and systems for sequencing reagents |
WO2023278184A1 (en) | 2021-06-29 | 2023-01-05 | Illumina, Inc. | Methods and systems to correct crosstalk in illumination emitted from reaction sites |
MX2023015504A (en) | 2021-06-29 | 2024-01-22 | Illumina Inc | Signal-to-noise-ratio metric for determining nucleotide-base calls and base-call quality. |
EP4364155A1 (en) | 2021-06-29 | 2024-05-08 | Illumina, Inc. | Self-learned base caller, trained using oligo sequences |
CA3224393A1 (en) | 2021-06-29 | 2023-01-05 | Mitchell A BEKRITSKY | Machine-learning model for generating confidence classifications for genomic coordinates |
US20230005253A1 (en) | 2021-07-01 | 2023-01-05 | Illumina, Inc. | Efficient artificial intelligence-based base calling of index sequences |
US20230027409A1 (en) | 2021-07-13 | 2023-01-26 | Illumina, Inc. | Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites |
US11455487B1 (en) | 2021-10-26 | 2022-09-27 | Illumina Software, Inc. | Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling |
JP2024532004A (en) | 2021-07-19 | 2024-09-04 | イルミナ インコーポレイテッド | Intensity extraction by interpolation and fitting for base calling |
US20230021577A1 (en) | 2021-07-23 | 2023-01-26 | Illumina Software, Inc. | Machine-learning model for recalibrating nucleotide-base calls |
WO2023004357A1 (en) | 2021-07-23 | 2023-01-26 | Illumina, Inc. | Methods for preparing substrate surface for dna sequencing |
JP2024532049A (en) | 2021-07-28 | 2024-09-05 | イルミナ インコーポレイテッド | Quality score calibration of base calling systems |
KR20240035413A (en) | 2021-08-03 | 2024-03-15 | 일루미나, 인코포레이티드 | Base calling using the multi-base caller model |
US12077789B2 (en) | 2021-08-14 | 2024-09-03 | Illumina, Inc. | Polymerases, compositions, and methods of use |
CN117881795A (en) | 2021-08-17 | 2024-04-12 | 因美纳有限公司 | Methods and compositions for identifying methylated cytosines |
WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
CN117561573A (en) | 2021-09-17 | 2024-02-13 | 因美纳有限公司 | Automatic identification of the source of faults in nucleotide sequencing from base interpretation error patterns |
KR20240072970A (en) | 2021-09-21 | 2024-05-24 | 일루미나, 인코포레이티드 | Graph reference genome and base determination approaches using imputed haplotypes. |
WO2023049215A1 (en) | 2021-09-22 | 2023-03-30 | Illumina, Inc. | Compressed state-based base calling |
WO2023056328A2 (en) | 2021-09-30 | 2023-04-06 | Illumina, Inc. | Solid supports and methods for depleting and/or enriching library fragments prepared from biosamples |
US20230096386A1 (en) | 2021-09-30 | 2023-03-30 | Illumina Cambridge Limited | Polynucleotide sequencing |
CA3232183A1 (en) | 2021-10-11 | 2023-04-20 | Nautilus Subsidiary, Inc. | Highly multiplexable analysis of proteins and proteomes |
EP4419705A1 (en) | 2021-10-20 | 2024-08-28 | Illumina, Inc. | Methods for capturing library dna for sequencing |
WO2023081485A1 (en) | 2021-11-08 | 2023-05-11 | Pacific Biosciences Of California, Inc. | Stepwise sequencing of a polynucleotide with a homogenous reaction mixture |
CN117581303A (en) | 2021-12-02 | 2024-02-20 | 因美纳有限公司 | Generating cluster-specific signal corrections for determining nucleotide base detection |
US20230215515A1 (en) | 2021-12-23 | 2023-07-06 | Illumina Software, Inc. | Facilitating secure execution of external workflows for genomic sequencing diagnostics |
WO2023122363A1 (en) | 2021-12-23 | 2023-06-29 | Illumina Software, Inc. | Dynamic graphical status summaries for nucelotide sequencing |
US20230207050A1 (en) | 2021-12-28 | 2023-06-29 | Illumina Software, Inc. | Machine learning model for recalibrating nucleotide base calls corresponding to target variants |
KR20240131386A (en) | 2021-12-29 | 2024-08-30 | 일루미나, 인코포레이티드 | Automatic switching of variant analysis model versions for genomic analysis applications |
AU2023208743A1 (en) | 2022-01-20 | 2024-01-04 | Illumina, Inc. | Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing |
AU2023225949A1 (en) | 2022-02-25 | 2024-01-18 | Illumina, Inc. | Machine-learning models for detecting and adjusting values for nucleotide methylation levels |
CN118871994A (en) | 2022-02-25 | 2024-10-29 | 因美纳有限公司 | Calibration sequence for nucleotide sequencing |
WO2023183937A1 (en) | 2022-03-25 | 2023-09-28 | Illumina, Inc. | Sequence-to-sequence base calling |
US12092578B2 (en) | 2022-03-29 | 2024-09-17 | Nautilus Subsidiary, Inc. | Integrated arrays for single-analyte processes |
CA3223722A1 (en) | 2022-04-07 | 2023-10-12 | Illumina, Inc. | Altered cytidine deaminases and methods of use |
WO2023212490A1 (en) | 2022-04-25 | 2023-11-02 | Nautilus Subsidiary, Inc. | Systems and methods for assessing and improving the quality of multiplex molecular assays |
WO2023212601A1 (en) | 2022-04-26 | 2023-11-02 | Illumina, Inc. | Machine-learning models for selecting oligonucleotide probes for array technologies |
WO2023209606A1 (en) | 2022-04-29 | 2023-11-02 | Illumina Cambridge Limited | Methods and systems for encapsulating lyophilised microspheres |
WO2023220627A1 (en) | 2022-05-10 | 2023-11-16 | Illumina Software, Inc. | Adaptive neural network for nucelotide sequencing |
WO2023235353A2 (en) | 2022-06-03 | 2023-12-07 | Illumina, Inc. | Circulating rna biomarkers for preeclampsia |
WO2023239917A1 (en) | 2022-06-09 | 2023-12-14 | Illumina, Inc. | Dependence of base calling on flow cell tilt |
US20230420080A1 (en) | 2022-06-24 | 2023-12-28 | Illumina Software, Inc. | Split-read alignment by intelligently identifying and scoring candidate split groups |
WO2024006769A1 (en) | 2022-06-27 | 2024-01-04 | Illumina Software, Inc. | Generating and implementing a structural variation graph genome |
US20230420075A1 (en) | 2022-06-27 | 2023-12-28 | Illumina Software, Inc. | Accelerators for a genotype imputation model |
WO2024006705A1 (en) | 2022-06-27 | 2024-01-04 | Illumina Software, Inc. | Improved human leukocyte antigen (hla) genotyping |
WO2024015962A1 (en) | 2022-07-15 | 2024-01-18 | Pacific Biosciences Of California, Inc. | Blocked asymmetric hairpin adaptors |
US20240038327A1 (en) | 2022-07-26 | 2024-02-01 | Illumina Software, Inc. | Rapid single-cell multiomics processing using an executable file |
WO2024039516A1 (en) | 2022-08-19 | 2024-02-22 | Illumina, Inc. | Third dna base pair site-specific dna detection |
WO2024059655A1 (en) | 2022-09-15 | 2024-03-21 | Nautilus Subsidiary, Inc. | Characterizing accessibility of macromolecule structures |
WO2024073349A2 (en) * | 2022-09-26 | 2024-04-04 | Ansa Biotechnologies, Inc. | Cleavable linkers for the tethering of polymerases to nucleotides |
US20240112753A1 (en) | 2022-09-29 | 2024-04-04 | Illumina, Inc. | Target-variant-reference panel for imputing target variants |
WO2024073043A1 (en) | 2022-09-30 | 2024-04-04 | Illumina, Inc. | Methods of using cpg binding proteins in mapping modified cytosine nucleotides |
US20240120027A1 (en) | 2022-09-30 | 2024-04-11 | Illumina, Inc. | Machine-learning model for refining structural variant calls |
WO2024073047A1 (en) | 2022-09-30 | 2024-04-04 | Illumina, Inc. | Cytidine deaminases and methods of use in mapping modified cytosine nucleotides |
WO2024069581A1 (en) | 2022-09-30 | 2024-04-04 | Illumina Singapore Pte. Ltd. | Helicase-cytidine deaminase complexes and methods of use |
US20240141427A1 (en) | 2022-09-30 | 2024-05-02 | Illumina, Inc. | Polymerases, compositions, and methods of use |
US20240127905A1 (en) | 2022-10-05 | 2024-04-18 | Illumina, Inc. | Integrating variant calls from multiple sequencing pipelines utilizing a machine learning architecture |
WO2024077152A1 (en) | 2022-10-06 | 2024-04-11 | Illumina, Inc. | Probes for depleting abundant small noncoding rna |
WO2024077162A2 (en) | 2022-10-06 | 2024-04-11 | Illumina, Inc. | Probes for improving coronavirus sample surveillance |
WO2024077202A2 (en) | 2022-10-06 | 2024-04-11 | Illumina, Inc. | Probes for improving environmental sample surveillance |
WO2024081649A1 (en) | 2022-10-11 | 2024-04-18 | Illumina, Inc. | Detecting and correcting methylation values from methylation sequencing assays |
WO2024118791A1 (en) | 2022-11-30 | 2024-06-06 | Illumina, Inc. | Accurately predicting variants from methylation sequencing data |
WO2024118903A1 (en) | 2022-11-30 | 2024-06-06 | Illumina, Inc. | Chemoenzymatic correction of false positive uracil transformations |
WO2024129672A1 (en) | 2022-12-12 | 2024-06-20 | The Broad Institute, Inc. | Trafficked rnas for assessment of cell-cell connectivity and neuroanatomy |
US20240209419A1 (en) | 2022-12-14 | 2024-06-27 | Illumina, Inc. | Systems and Methods for Capture and Enrichment of Clustered Beads on Flow Cell Substrates |
WO2024130031A1 (en) | 2022-12-16 | 2024-06-20 | Illumina, Inc. | Boranes on solid supports |
WO2024137774A1 (en) | 2022-12-22 | 2024-06-27 | Illumina, Inc. | Palladium catalyst compositions and methods for sequencing by synthesis |
AU2023409138A1 (en) | 2022-12-22 | 2024-10-03 | Illumina, Inc. | Transition-metal catalyst compositions and methods for sequencing by synthesis |
WO2024145154A1 (en) | 2022-12-27 | 2024-07-04 | Illumina, Inc. | Methods of sequencing using 3´ allyl blocked nucleotides |
WO2024147904A1 (en) | 2023-01-06 | 2024-07-11 | Illumina, Inc. | Reducing uracils by polymerase |
US20240241110A1 (en) | 2023-01-12 | 2024-07-18 | Nautilus Subsidiary, Inc. | Characterization of glycans and glycoconjugates |
US20240266003A1 (en) | 2023-02-06 | 2024-08-08 | Illumina, Inc. | Determining and removing inter-cluster light interference |
WO2024173756A1 (en) | 2023-02-17 | 2024-08-22 | Illumina, Inc. | Cell-free dna signals as biomarkers of preeclampsia |
US20240327909A1 (en) | 2023-03-30 | 2024-10-03 | Illumina, Inc. | Compositions and methods for nucleic acid sequencing |
WO2024206848A1 (en) | 2023-03-30 | 2024-10-03 | Illumina, Inc. | Tandem repeat genotyping |
Family Cites Families (358)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
US4351760A (en) | 1979-09-07 | 1982-09-28 | Syva Company | Novel alkyl substituted fluorescent compounds and polyamino acid conjugates |
SE429036B (en) | 1979-12-17 | 1983-08-08 | Haustrup Plastic As | CONTAINER WITH A CLOSING PART |
US4711955A (en) | 1981-04-17 | 1987-12-08 | Yale University | Modified nucleotides and methods of preparing and using same |
NO155316C (en) | 1982-04-23 | 1987-03-11 | Sintef | PROCEDURE FOR MAKING MAGNETIC POLYMER PARTICLES. |
US5241060A (en) * | 1982-06-23 | 1993-08-31 | Enzo Diagnostics, Inc. | Base moiety-labeled detectable nucleatide |
US4994373A (en) | 1983-01-27 | 1991-02-19 | Enzo Biochem, Inc. | Method and structures employing chemically-labelled polynucleotide probes |
US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
IT1172599B (en) | 1983-11-09 | 1987-06-18 | Santarelli Franco | MECHANISM TO OPERATE THE DISC BRAKES OF MOTOR VEHICLES OR OTHER |
US5171534A (en) | 1984-01-16 | 1992-12-15 | California Institute Of Technology | Automated DNA sequencing technique |
US5821058A (en) | 1984-01-16 | 1998-10-13 | California Institute Of Technology | Automated DNA sequencing technique |
US4729947A (en) | 1984-03-29 | 1988-03-08 | The Board Of Regents Of The University Of Nebraska | DNA sequencing |
US5360523A (en) | 1984-03-29 | 1994-11-01 | Li-Cor, Inc. | DNA sequencing |
US6086737A (en) | 1984-03-29 | 2000-07-11 | Li-Cor, Inc. | Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes and suitable labels therefor |
US5571388A (en) | 1984-03-29 | 1996-11-05 | Li-Cor, Inc. | Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof |
US6207421B1 (en) | 1984-03-29 | 2001-03-27 | Li-Cor, Inc. | DNA sequencing and DNA terminators |
US5366603A (en) | 1984-03-29 | 1994-11-22 | Li-Cor, Inc. | Sequencing near infrared and infrared fluorescence labeled DNA for detecting useing laser diodes |
US6004446A (en) | 1984-03-29 | 1999-12-21 | Li-Cor, Inc. | DNA Sequencing |
US5230781A (en) | 1984-03-29 | 1993-07-27 | Li-Cor, Inc. | Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4739044A (en) * | 1985-06-13 | 1988-04-19 | Amgen | Method for derivitization of polynucleotides |
US4757141A (en) | 1985-08-26 | 1988-07-12 | Applied Biosystems, Incorporated | Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof |
US4764460A (en) | 1985-11-08 | 1988-08-16 | Hoffmann-La Roche Inc. | Recombinant sigma NS protein |
US4800159A (en) * | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
CA1340806C (en) | 1986-07-02 | 1999-11-02 | James Merrill Prober | Method, system and reagents for dna sequencing |
US5047519A (en) | 1986-07-02 | 1991-09-10 | E. I. Du Pont De Nemours And Company | Alkynylamino-nucleotides |
US5151507A (en) | 1986-07-02 | 1992-09-29 | E. I. Du Pont De Nemours And Company | Alkynylamino-nucleotides |
US5849478A (en) | 1986-08-14 | 1998-12-15 | Cashman; Daniel P. | Blocked-polymerase polynucleotide immunoassay method and kit |
CA1338457C (en) | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
US5079352A (en) * | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
JP2527340B2 (en) | 1986-12-15 | 1996-08-21 | アプライド バイオシステムズ インコーポレイテッド | 5- and 6-succinimidyl carboxylate isomers of rhodamine dyes |
US5202231A (en) | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
US4962037A (en) | 1987-10-07 | 1990-10-09 | United States Of America | Method for rapid base sequencing in DNA and RNA |
US4793705A (en) | 1987-10-07 | 1988-12-27 | The United States Of America As Represented By The United States Department Of Energy | Single molecule tracking |
US5244797B1 (en) * | 1988-01-13 | 1998-08-25 | Life Technologies Inc | Cloned genes encoding reverse transcriptase lacking rnase h activity |
US4971903A (en) | 1988-03-25 | 1990-11-20 | Edward Hyman | Pyrophosphate-based method and apparatus for sequencing nucleic acids |
US4962020A (en) | 1988-07-12 | 1990-10-09 | President And Fellows Of Harvard College | DNA sequencing |
US5498523A (en) * | 1988-07-12 | 1996-03-12 | President And Fellows Of Harvard College | DNA sequencing with pyrophosphatase |
US4997928A (en) | 1988-09-15 | 1991-03-05 | E. I. Du Pont De Nemours And Company | Fluorescent reagents for the preparation of 5'-tagged oligonucleotides |
US5720928A (en) * | 1988-09-15 | 1998-02-24 | New York University | Image processing and analysis of individual nucleic acid molecules |
US5091310A (en) | 1988-09-23 | 1992-02-25 | Cetus Corporation | Structure-independent dna amplification by the polymerase chain reaction |
FR2641793B1 (en) | 1988-12-26 | 1993-10-01 | Setratech | METHOD OF IN VIVO RECOMBINATION OF DNA SEQUENCES HAVING BASIC MATCHING |
KR920700360A (en) | 1989-03-22 | 1992-02-19 | 하리크 프리드리히 | Sliding bearing |
US5001050A (en) | 1989-03-24 | 1991-03-19 | Consejo Superior Investigaciones Cientificas | PHφ29 DNA polymerase |
US5198543A (en) * | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
GB8910880D0 (en) | 1989-05-11 | 1989-06-28 | Amersham Int Plc | Sequencing method |
US5198060A (en) * | 1989-05-15 | 1993-03-30 | Stephen Kurtin | Method for replicating solvent-sensitive images |
US4979824A (en) | 1989-05-26 | 1990-12-25 | Board Of Trustees Of The Leland Stanford Junior University | High sensitivity fluorescent single particle and single molecule detection apparatus and method |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5547839A (en) * | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
IT1231157B (en) | 1989-07-20 | 1991-11-19 | Crc Ricerca Chim | NEW FUNCTIONAL HYBRID GENES OF BACILLUS THURINGIENSIS OBTAINED THROUGH IN VIVO RECOMBINATION. |
US5302509A (en) | 1989-08-14 | 1994-04-12 | Beckman Instruments, Inc. | Method for sequencing polynucleotides |
US6984487B1 (en) | 1989-08-22 | 2006-01-10 | Hsc Research Development Corporation | Cystic fibrosis gene |
GB8920571D0 (en) | 1989-09-12 | 1989-10-25 | Carr Robert J G | Examination of objects of macromolecular size |
WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
US5188934A (en) * | 1989-11-14 | 1993-02-23 | Applied Biosystems, Inc. | 4,7-dichlorofluorescein dyes as molecular probes |
US5091652A (en) | 1990-01-12 | 1992-02-25 | The Regents Of The University Of California | Laser excited confocal microscope fluorescence scanner and method |
US5274240A (en) | 1990-01-12 | 1993-12-28 | The Regents Of The University Of California | Capillary array confocal fluorescence scanner and method |
IL97222A (en) | 1990-02-16 | 1995-08-31 | Orion Yhtymae Oy | Method and reagent for determining specific nucleotide variations |
US5401847A (en) | 1990-03-14 | 1995-03-28 | Regents Of The University Of California | DNA complexes with dyes designed for energy transfer as fluorescent markers |
US5227487A (en) | 1990-04-16 | 1993-07-13 | Molecular Probes, Inc. | Certain tricyclic and pentacyclic-hetero nitrogen rhodol dyes |
US5079169A (en) * | 1990-05-22 | 1992-01-07 | The Regents Of The Stanford Leland Junior University | Method for optically manipulating polymer filaments |
WO1992010588A1 (en) | 1990-12-06 | 1992-06-25 | Affymax Technologies N.V. | Sequencing by hybridization of a target nucleic acid to a matrix of defined oligonucleotides |
US6004744A (en) | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
US5578443A (en) | 1991-03-06 | 1996-11-26 | Regents Of The University Of Minnesota | DNA sequence-based HLA typing method |
US5119463A (en) | 1991-04-09 | 1992-06-02 | Abbott Laboratories | Compound optical probe employing single optical waveguide |
JP3123249B2 (en) | 1991-09-10 | 2001-01-09 | 株式会社日立製作所 | DNA molecule length measuring method and measuring device |
US5405747A (en) * | 1991-09-25 | 1995-04-11 | The Regents Of The University Of California Office Of Technology Transfer | Method for rapid base sequencing in DNA and RNA with two base labeling |
US6048690A (en) * | 1991-11-07 | 2000-04-11 | Nanogen, Inc. | Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis |
US5605662A (en) * | 1993-11-01 | 1997-02-25 | Nanogen, Inc. | Active programmable electronic devices for molecular biological analysis and diagnostics |
JPH05244997A (en) | 1992-03-04 | 1993-09-24 | Hitachi Ltd | Method for determining base sequence of dna or rna |
WO1993019205A1 (en) * | 1992-03-19 | 1993-09-30 | The Regents Of The University Of California | Multiple tag labeling method for dna sequencing |
US5470705A (en) | 1992-04-03 | 1995-11-28 | Applied Biosystems, Inc. | Probe composition containing a binding domain and polymer chain and methods of use |
GB9208733D0 (en) | 1992-04-22 | 1992-06-10 | Medical Res Council | Dna sequencing method |
GB9210168D0 (en) | 1992-05-12 | 1992-06-24 | Cemu Bioteknik Ab | Method of sequencing dna |
US5403708A (en) | 1992-07-06 | 1995-04-04 | Brennan; Thomas M. | Methods and compositions for determining the sequence of nucleic acids |
US5503980A (en) | 1992-11-06 | 1996-04-02 | Trustees Of Boston University | Positional sequencing by hybridization |
JP2575270B2 (en) | 1992-11-10 | 1997-01-22 | 浜松ホトニクス株式会社 | Method for determining base sequence of nucleic acid, method for detecting single molecule, apparatus therefor and method for preparing sample |
US5547835A (en) | 1993-01-07 | 1996-08-20 | Sequenom, Inc. | DNA sequencing by mass spectrometry |
WO1994016104A1 (en) | 1993-01-08 | 1994-07-21 | Ctrc Research Foundation | Color imaging system for use in molecular biology |
EP0682671A4 (en) | 1993-02-01 | 1998-01-14 | Seq Ltd | Methods and apparatus for dna sequencing. |
US5324401A (en) * | 1993-02-05 | 1994-06-28 | Iowa State University Research Foundation, Inc. | Multiplexed fluorescence detector system for capillary electrophoresis |
US5403707A (en) * | 1993-05-14 | 1995-04-04 | Eastman Kodak Company | Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures |
US5677196A (en) | 1993-05-18 | 1997-10-14 | University Of Utah Research Foundation | Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays |
US5424841A (en) | 1993-05-28 | 1995-06-13 | Molecular Dynamics | Apparatus for measuring spatial distribution of fluorescence on a substrate |
US5473060A (en) | 1993-07-02 | 1995-12-05 | Lynx Therapeutics, Inc. | Oligonucleotide clamps having diagnostic applications |
US5706805A (en) | 1993-08-12 | 1998-01-13 | Trustees Of Dartmouth College | Apparatus and methodology for determining oxygen tension in biological systems |
US5494030A (en) * | 1993-08-12 | 1996-02-27 | Trustees Of Dartmouth College | Apparatus and methodology for determining oxygen in biological systems |
US5817632A (en) | 1993-08-16 | 1998-10-06 | Hsia; Jen-Chang | Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules |
US5725839A (en) * | 1993-08-16 | 1998-03-10 | Hsia; Jen-Chang | Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules for ERI or MRI |
US6458758B1 (en) | 1993-08-16 | 2002-10-01 | Synzyme Technologies, Inc. | Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules |
US5840701A (en) | 1993-08-16 | 1998-11-24 | Hsia; Jen-Chang | Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules |
US5804561A (en) | 1993-08-16 | 1998-09-08 | Hsia; Jen-Chang | Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules |
US5807831A (en) | 1993-08-16 | 1998-09-15 | Hsia; Jen-Chang | Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules |
US5824781A (en) | 1993-08-16 | 1998-10-20 | Hsia; Jen-Chang | Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules |
US5741893A (en) * | 1993-08-16 | 1998-04-21 | Hsia; Jen-Chang | Compositions and methods utilizing nitroxides in combination with biocompatible macromolecules |
WO1995006138A1 (en) | 1993-08-25 | 1995-03-02 | The Regents Of The University Of California | Microscopic method for detecting micromotions |
US5470710A (en) | 1993-10-22 | 1995-11-28 | University Of Utah | Automated hybridization/imaging device for fluorescent multiplex DNA sequencing |
US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
CA2174863A1 (en) * | 1993-11-26 | 1995-06-01 | Yuti Luis Alberto Chernajovsky | Translational enhancer dna |
JPH07203998A (en) | 1994-01-26 | 1995-08-08 | Hamamatsu Photonics Kk | Method for determining nucleic acid base sequence |
US5654419A (en) | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
US5869255A (en) * | 1994-02-01 | 1999-02-09 | The Regents Of The University Of California | Probes labeled with energy transfer couples dyes exemplified with DNA fragment analysis |
US5631734A (en) | 1994-02-10 | 1997-05-20 | Affymetrix, Inc. | Method and apparatus for detection of fluorescently labeled materials |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
US6593148B1 (en) | 1994-03-01 | 2003-07-15 | Li-Cor, Inc. | Cyanine dye compounds and labeling methods |
US5552278A (en) * | 1994-04-04 | 1996-09-03 | Spectragen, Inc. | DNA sequencing by stepwise ligation and cleavage |
US5459325A (en) | 1994-07-19 | 1995-10-17 | Molecular Dynamics, Inc. | High-speed fluorescence scanner |
PT721505E (en) * | 1994-07-29 | 2002-10-31 | Innogenetics Nv | HEPATITIS C VIRUS ENVELOPE PROTEINS PURIFIED FOR USE IN DIAGNOSIS AND THERAPEUTICS |
US5695934A (en) | 1994-10-13 | 1997-12-09 | Lynx Therapeutics, Inc. | Massively parallel sequencing of sorted polynucleotides |
SE9500342D0 (en) * | 1995-01-31 | 1995-01-31 | Marek Kwiatkowski | Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation |
US5601982A (en) * | 1995-02-07 | 1997-02-11 | Sargent; Jeannine P. | Method and apparatus for determining the sequence of polynucleotides |
AU5171696A (en) | 1995-02-27 | 1996-09-18 | Ely Michael Rabani | Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism |
US5599695A (en) | 1995-02-27 | 1997-02-04 | Affymetrix, Inc. | Printing molecular library arrays using deprotection agents solely in the vapor phase |
US5538898A (en) * | 1995-03-16 | 1996-07-23 | International Business Machines Corporation | Method suitable for identifying a code sequence of a biomolecule |
US5795782A (en) * | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
US6362002B1 (en) * | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
WO1996030508A1 (en) | 1995-03-24 | 1996-10-03 | Ely Michael Rabani | Assembly of complex molecular and supramolecular objects and devices and uses thereof |
US5707807A (en) * | 1995-03-28 | 1998-01-13 | Research Development Corporation Of Japan | Molecular indexing for expressed gene analysis |
US5961923A (en) | 1995-04-25 | 1999-10-05 | Irori | Matrices with memories and uses thereof |
DE19515552A1 (en) | 1995-04-27 | 1996-10-31 | Europ Lab Molekularbiolog | Simultaneous sequencing of nucleic acids |
AU5797696A (en) | 1995-05-19 | 1996-11-29 | Ely Michael Rabani | Parallel multiplex polynucleotide sequencing |
EP0745686A1 (en) | 1995-06-01 | 1996-12-04 | Roche Diagnostics GmbH | The use of DNA polymerase 3'-intrinsic editing activity |
US5776674A (en) | 1995-06-05 | 1998-07-07 | Seq, Ltd | Chemical biochemical and biological processing in thin films |
US5635728A (en) | 1995-06-19 | 1997-06-03 | Denoptix, Inc. | Rotating scanner system for reading multiple storage layer radiation screens |
DE29509993U1 (en) | 1995-06-20 | 1995-09-14 | Textilma Ag, Hergiswil | Plant for the production of at least one knitted band with crocheted rubber threads |
US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
AU6898296A (en) | 1995-08-14 | 1997-03-12 | Ely Michael Rabani | Methods and devices for parallel multiplex polynucleotide sequencing |
US5661028A (en) | 1995-09-29 | 1997-08-26 | Lockheed Martin Energy Systems, Inc. | Large scale DNA microsequencing device |
US6165765A (en) | 1995-10-18 | 2000-12-26 | Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences | DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides |
US5814524A (en) | 1995-12-14 | 1998-09-29 | Trustees Of Tufts College | Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations |
US6027890A (en) | 1996-01-23 | 2000-02-22 | Rapigene, Inc. | Methods and compositions for enhancing sensitivity in the analysis of biological-based assays |
US5972603A (en) | 1996-02-09 | 1999-10-26 | President And Fellows Of Harvard College | DNA polymerase with modified processivity |
US5715534A (en) * | 1996-02-23 | 1998-02-10 | Mobley; Gwennette Q. | Hats and caps with moveable bills or brims |
US6020481A (en) * | 1996-04-01 | 2000-02-01 | The Perkin-Elmer Corporation | Asymmetric benzoxanthene dyes |
US6162931A (en) | 1996-04-12 | 2000-12-19 | Molecular Probes, Inc. | Fluorinated xanthene derivatives |
CA2251643A1 (en) | 1996-04-15 | 1997-10-23 | University Of Alberta | Synthesis of fluorophore-labeled dna |
US6083695A (en) | 1996-04-15 | 2000-07-04 | The University Of Houston | Optimized primer library for gene sequencing and method of using same |
US5863727A (en) * | 1996-05-03 | 1999-01-26 | The Perkin-Elmer Corporation | Energy transfer dyes with enhanced fluorescence |
US5800996A (en) | 1996-05-03 | 1998-09-01 | The Perkin Elmer Corporation | Energy transfer dyes with enchanced fluorescence |
US5846727A (en) | 1996-06-06 | 1998-12-08 | Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College | Microsystem for rapid DNA sequencing |
US5866336A (en) * | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
US5906723A (en) | 1996-08-26 | 1999-05-25 | The Regents Of The University Of California | Electrochemical detector integrated on microfabricated capillary electrophoresis chips |
WO1998010097A2 (en) | 1996-09-03 | 1998-03-12 | Pioneer Hi-Bred International, Inc. | Method and apparatus for single molecule two color fluorescent detection and molecular weight and concentration determination |
US5830659A (en) | 1996-09-13 | 1998-11-03 | University Of Utah Research Foundation | Active microtubule-based separations by kinesins |
US5723298A (en) | 1996-09-16 | 1998-03-03 | Li-Cor, Inc. | Cycle labeling and sequencing with thermostable polymerases |
GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
EE9600101A (en) | 1996-09-27 | 1998-04-15 | Tartu Ülikool | Method and method for determining the nucleotide sequence of DNA by laser spectroscopy |
US5858671A (en) * | 1996-11-01 | 1999-01-12 | The University Of Iowa Research Foundation | Iterative and regenerative DNA sequencing method |
US6291164B1 (en) | 1996-11-22 | 2001-09-18 | Invitrogen Corporation | Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate |
GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
US20030104372A1 (en) * | 1996-12-23 | 2003-06-05 | Pyrosequencing Ab. | Allele specific primer extension |
US6027709A (en) * | 1997-01-10 | 2000-02-22 | Li-Cor Inc. | Fluorescent cyanine dyes |
FR2758262B1 (en) | 1997-01-14 | 2004-11-19 | Oreal | COSMETIC OR DERMATOLOGICAL COMPOSITION IN THE FORM OF A GEL CONTAINING A MIXTURE OF AN ASSOCIATIVE COPOLYMER, A SURFACTANT AND AN INSOLUBLE CONDITIONING AGENT |
US5804386A (en) | 1997-01-15 | 1998-09-08 | Incyte Pharmaceuticals, Inc. | Sets of labeled energy transfer fluorescent primers and their use in multi component analysis |
US6136543A (en) | 1997-01-31 | 2000-10-24 | Hitachi, Ltd. | Method for determining nucleic acids base sequence and apparatus therefor |
EP1009802B1 (en) * | 1997-02-12 | 2004-08-11 | Eugene Y. Chan | Methods for analyzimg polymers |
US6403311B1 (en) * | 1997-02-12 | 2002-06-11 | Us Genomics | Methods of analyzing polymers using ordered label strategies |
WO1998044152A1 (en) | 1997-04-01 | 1998-10-08 | Glaxo Group Limited | Method of nucleic acid sequencing |
US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
US5928869A (en) * | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
KR100414142B1 (en) * | 1997-06-05 | 2004-05-07 | 간사이 페인트 가부시키가이샤 | Phosphoric acid-containing nonaqueous acid solution and its coating method |
US5888792A (en) | 1997-07-11 | 1999-03-30 | Incyte Pharmaceuticals, Inc. | ATP-dependent RNA helicase protein |
JP2001511358A (en) | 1997-07-28 | 2001-08-14 | メディカル・バイオシステムズ・リミテッド | Analysis of nucleic acid sequence |
US6245506B1 (en) | 1997-07-30 | 2001-06-12 | Bbi Bioseq, Inc. | Integrated sequencing device |
JP2001513983A (en) | 1997-08-29 | 2001-09-11 | ライフ テクノロジーズ,インコーポレイテッド | High fidelity polymerase and its use |
US6130101A (en) | 1997-09-23 | 2000-10-10 | Molecular Probes, Inc. | Sulfonated xanthene derivatives |
US5932442A (en) * | 1997-09-23 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Human regulatory molecules |
US6399334B1 (en) * | 1997-09-24 | 2002-06-04 | Invitrogen Corporation | Normalized nucleic acid libraries and methods of production thereof |
US6008379A (en) | 1997-10-01 | 1999-12-28 | The Perkin-Elmer Corporation | Aromatic-substituted xanthene dyes |
US6485944B1 (en) | 1997-10-10 | 2002-11-26 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
EP1028970A1 (en) | 1997-10-10 | 2000-08-23 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
US7098012B1 (en) | 1997-11-12 | 2006-08-29 | Mcgill University | DNA demethylase, therapeutic and diagnostic uses thereof |
US6322901B1 (en) * | 1997-11-13 | 2001-11-27 | Massachusetts Institute Of Technology | Highly luminescent color-selective nano-crystalline materials |
US6207392B1 (en) * | 1997-11-25 | 2001-03-27 | The Regents Of The University Of California | Semiconductor nanocrystal probes for biological applications and process for making and using such probes |
US5936087A (en) * | 1997-11-25 | 1999-08-10 | The Perkin-Elmer Corporation | Dibenzorhodamine dyes |
US5990479A (en) * | 1997-11-25 | 1999-11-23 | Regents Of The University Of California | Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes |
GB9803382D0 (en) | 1998-02-19 | 1998-04-15 | Secr Defence | Detection system |
WO1999044045A1 (en) | 1998-02-27 | 1999-09-02 | Massachusetts Institute Of Technology | Single molecule detection with surface-enhanced raman scattering and applications in dna or rna sequencing |
AU3487799A (en) | 1998-04-09 | 1999-11-01 | University Of Nebraska Board Of Regents | Improved live attenuated viruses for use as vectors or vaccines |
CA2330574A1 (en) | 1998-04-29 | 1999-11-04 | Ribozyme Pharmaceuticals, Inc. | Nucleoside triphosphates and their incorporation into ribozymes |
US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
WO1999057321A1 (en) * | 1998-05-01 | 1999-11-11 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and dna molecules |
US6287821B1 (en) * | 1998-06-11 | 2001-09-11 | Orchid Biosciences, Inc. | Nucleotide analogues with 3'-pro-fluorescent fluorophores in nucleic acid sequence analysis |
AU4999099A (en) | 1998-07-15 | 2000-02-07 | Ljl Biosystems, Inc. | Evanescent field illumination devices and methods |
WO2000006587A1 (en) | 1998-07-29 | 2000-02-10 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | New cofactors for methyltransferases |
JP2002521064A (en) | 1998-07-30 | 2002-07-16 | ソレックサ リミテッド | Array biomolecules and their use in sequencing |
GB0002310D0 (en) | 2000-02-01 | 2000-03-22 | Solexa Ltd | Polynucleotide sequencing |
US6787308B2 (en) | 1998-07-30 | 2004-09-07 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
US6210896B1 (en) | 1998-08-13 | 2001-04-03 | Us Genomics | Molecular motors |
CN1323355A (en) | 1998-08-13 | 2001-11-21 | 美国吉诺米克斯公司 | Optically characterizing polymers |
US6790671B1 (en) | 1998-08-13 | 2004-09-14 | Princeton University | Optically characterizing polymers |
US6263286B1 (en) | 1998-08-13 | 2001-07-17 | U.S. Genomics, Inc. | Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer |
US6280939B1 (en) | 1998-09-01 | 2001-08-28 | Veeco Instruments, Inc. | Method and apparatus for DNA sequencing using a local sensitive force detector |
DE19844931C1 (en) | 1998-09-30 | 2000-06-15 | Stefan Seeger | Procedures for DNA or RNA sequencing |
US6221592B1 (en) * | 1998-10-20 | 2001-04-24 | Wisconsin Alumi Research Foundation | Computer-based methods and systems for sequencing of individual nucleic acid molecules |
CA2355816C (en) * | 1998-12-14 | 2007-10-30 | Li-Cor, Inc. | A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis |
GB9828785D0 (en) | 1998-12-30 | 1999-02-17 | Amersham Pharm Biotech Ab | Sequencing systems |
GB9901475D0 (en) | 1999-01-22 | 1999-03-17 | Pyrosequencing Ab | A method of DNA sequencing |
AU3498900A (en) | 1999-02-23 | 2000-09-14 | Caliper Technologies Corporation | Sequencing by incorporation |
US6558945B1 (en) | 1999-03-08 | 2003-05-06 | Aclara Biosciences, Inc. | Method and device for rapid color detection |
ES2310514T3 (en) | 1999-03-10 | 2009-01-16 | Asm Scientific, Inc. | A METHOD FOR DIRECT SEQUENCING OF NUCLEIC ACID. |
US6436640B1 (en) | 1999-03-18 | 2002-08-20 | Exiqon A/S | Use of LNA in mass spectrometry |
IL145417A0 (en) | 1999-03-18 | 2002-06-30 | Exiqon As | Detection of mutations in genes by specific lna primers |
CN1350591A (en) | 1999-03-18 | 2002-05-22 | 埃克西库恩公司 | One step sample preparation and detection of nucleic acids in complex biological samples |
WO2000058507A1 (en) | 1999-03-30 | 2000-10-05 | Solexa Ltd. | Polynucleotide sequencing |
GB9907812D0 (en) | 1999-04-06 | 1999-06-02 | Medical Biosystems Ltd | Sequencing |
GB9907813D0 (en) | 1999-04-06 | 1999-06-02 | Medical Biosystems Ltd | Synthesis |
US20030108867A1 (en) | 1999-04-20 | 2003-06-12 | Chee Mark S | Nucleic acid sequencing using microsphere arrays |
EP1090073B1 (en) | 1999-04-23 | 2003-03-05 | Molecular Probes Inc. | Xanthene dyes and their application as luminescence quenching compounds |
US6410255B1 (en) | 1999-05-05 | 2002-06-25 | Aurora Biosciences Corporation | Optical probes and assays |
JP4150166B2 (en) | 1999-05-07 | 2008-09-17 | ロシュ ダイアグノスティックス ゲーエムベーハー | High density labeling of DNA using modified polymerases or nucleotides with chromophores and DNA polymerase |
FR2793413B1 (en) | 1999-05-10 | 2008-11-07 | Centre Nat Rech Scient | USE OF NUCLEOTIDE ANALOGUES AS REVERSE TRANSCRIPTASE SUBSTRATE FOR THE TREATMENT OF CONDITIONS INVOLVING REVERSE-TRANSCRIPTION |
US7056661B2 (en) | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
US6635163B1 (en) | 1999-06-01 | 2003-10-21 | Cornell Research Foundation, Inc. | Entropic trapping and sieving of molecules |
US6054316A (en) * | 1999-06-25 | 2000-04-25 | Isis Pharmaceuticals Inc. | Antisense inhibition of ETs-2 expression |
US7501245B2 (en) * | 1999-06-28 | 2009-03-10 | Helicos Biosciences Corp. | Methods and apparatuses for analyzing polynucleotide sequences |
US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
IL148088A0 (en) | 1999-08-13 | 2002-09-12 | Us Genomics Inc | Methods and apparatuses for stretching polymers |
US6927065B2 (en) | 1999-08-13 | 2005-08-09 | U.S. Genomics, Inc. | Methods and apparatus for characterization of single polymers |
US6696022B1 (en) | 1999-08-13 | 2004-02-24 | U.S. Genomics, Inc. | Methods and apparatuses for stretching polymers |
US6762059B2 (en) | 1999-08-13 | 2004-07-13 | U.S. Genomics, Inc. | Methods and apparatuses for characterization of single polymers |
US6982146B1 (en) | 1999-08-30 | 2006-01-03 | The United States Of America As Represented By The Department Of Health And Human Services | High speed parallel molecular nucleic acid sequencing |
WO2001016375A2 (en) | 1999-08-30 | 2001-03-08 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | High speed parallel molecular nucleic acid sequencing |
US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
WO2001023610A2 (en) | 1999-09-29 | 2001-04-05 | Solexa Ltd. | Polynucleotide sequencing |
JP2003510063A (en) | 1999-09-30 | 2003-03-18 | ニュー・イングランド・バイオラブズ・インコーポレイティッド | Uptake of modified nucleotides by archaeal DNA polymerase and methods related thereto |
US6309836B1 (en) | 1999-10-05 | 2001-10-30 | Marek Kwiatkowski | Compounds for protecting hydroxyls and methods for their use |
GB9923644D0 (en) | 1999-10-06 | 1999-12-08 | Medical Biosystems Ltd | DNA sequencing |
US6908736B1 (en) | 1999-10-06 | 2005-06-21 | Medical Biosystems, Ltd. | DNA sequencing method |
US6372907B1 (en) | 1999-11-03 | 2002-04-16 | Apptera Corporation | Water-soluble rhodamine dye peptide conjugates |
CA2388528A1 (en) | 1999-11-04 | 2001-05-10 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
US6399335B1 (en) * | 1999-11-16 | 2002-06-04 | Advanced Research And Technology Institute, Inc. | γ-phosphoester nucleoside triphosphates |
US6329178B1 (en) | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
GB0002389D0 (en) | 2000-02-02 | 2000-03-22 | Solexa Ltd | Molecular arrays |
ES2330408T3 (en) * | 2000-02-23 | 2009-12-10 | City Of Hope | POLYMERIZATION ACTIVATED BY PYROPHOSPHOROLISIS (PAP): APPLICATION IN THE AMPLIFICATION OF A SPECIFIC ALELUS AND IN THE DETERMINATION OF NUCLEIC ACID SEQUENCES. |
CA2403620C (en) | 2000-03-20 | 2012-01-10 | Massachusetts Institute Of Technology | Inorganic chromophore bioconjugates |
US6917726B2 (en) | 2001-09-27 | 2005-07-12 | Cornell Research Foundation, Inc. | Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes |
US6627424B1 (en) | 2000-05-26 | 2003-09-30 | Mj Bioworks, Inc. | Nucleic acid modifying enzymes |
US20020168678A1 (en) | 2000-06-07 | 2002-11-14 | Li-Cor, Inc. | Flowcell system for nucleic acid sequencing |
US6869764B2 (en) * | 2000-06-07 | 2005-03-22 | L--Cor, Inc. | Nucleic acid sequencing using charge-switch nucleotides |
WO2001094609A1 (en) | 2000-06-07 | 2001-12-13 | Li-Cor, Inc. | Charge-switch nucleotides |
US6936702B2 (en) | 2000-06-07 | 2005-08-30 | Li-Cor, Inc. | Charge-switch nucleotides |
GB0016473D0 (en) | 2000-07-05 | 2000-08-23 | Amersham Pharm Biotech Uk Ltd | Sequencing method |
GB0016472D0 (en) | 2000-07-05 | 2000-08-23 | Amersham Pharm Biotech Uk Ltd | Sequencing method and apparatus |
US20070172866A1 (en) | 2000-07-07 | 2007-07-26 | Susan Hardin | Methods for sequence determination using depolymerizing agent |
AU2007216737A1 (en) | 2000-07-07 | 2007-10-04 | Visigen Biotechnologies, Inc. | Real-time sequence determination |
CN100462433C (en) | 2000-07-07 | 2009-02-18 | 维西根生物技术公司 | Real-time sequence determination |
GB0021977D0 (en) | 2000-09-07 | 2000-10-25 | Pyrosequencing Ab | Method of sequencing DNA |
US20060057565A1 (en) * | 2000-09-11 | 2006-03-16 | Jingyue Ju | Combinatorial fluorescence energy transfer tags and uses thereof |
EP1319047B1 (en) | 2000-09-19 | 2006-01-04 | Li-Cor, Inc. | Cyanine dyes |
US7597878B2 (en) | 2000-09-19 | 2009-10-06 | Li-Cor, Inc. | Optical fluorescent imaging |
JP3638516B2 (en) | 2000-09-28 | 2005-04-13 | 株式会社日立製作所 | Nucleic acid detection method and nucleic acid detection kit |
WO2002029106A2 (en) | 2000-10-03 | 2002-04-11 | California Institute Of Technology | Microfluidic devices and methods of use |
AU2002227156A1 (en) | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
EP1356119B1 (en) | 2001-01-30 | 2007-09-26 | Solexa Ltd. | The preparation of polynucleotide arrays |
US7297518B2 (en) | 2001-03-12 | 2007-11-20 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension |
US6514829B1 (en) * | 2001-03-12 | 2003-02-04 | Advanced Micro Devices, Inc. | Method of fabricating abrupt source/drain junctions |
US6960437B2 (en) | 2001-04-06 | 2005-11-01 | California Institute Of Technology | Nucleic acid amplification utilizing microfluidic devices |
US6320041B1 (en) | 2001-04-13 | 2001-11-20 | Trilink Biotechnologies, Inc. | Pre-activated carbonyl linkers for the modification of oligonucleotides |
US20040259082A1 (en) | 2001-04-24 | 2004-12-23 | Li-Cor, Inc. | Polymerases with charge-switch activity and methods of generating such polymers |
US7068773B2 (en) * | 2001-04-25 | 2006-06-27 | Tekelec | Methods and systems for load sharing signaling messages among signaling links in networks utilizing international signaling protocols |
GB0112238D0 (en) | 2001-05-18 | 2001-07-11 | Medical Biosystems Ltd | Sequencing method |
US7118907B2 (en) | 2001-06-06 | 2006-10-10 | Li-Cor, Inc. | Single molecule detection systems and methods |
JP2004536596A (en) | 2001-06-08 | 2004-12-09 | ユー.エス. ジェノミクス, インコーポレイテッド | Methods and products for analyzing nucleic acids based on methylation status |
EP1402069A4 (en) | 2001-06-08 | 2006-01-25 | Us Genomics Inc | Methods and products for analyzing nucleic acids using nick translation |
US20040161741A1 (en) | 2001-06-30 | 2004-08-19 | Elazar Rabani | Novel compositions and processes for analyte detection, quantification and amplification |
US7668697B2 (en) | 2006-02-06 | 2010-02-23 | Andrei Volkov | Method for analyzing dynamic detectable events at the single molecule level |
ATE556845T1 (en) | 2001-07-20 | 2012-05-15 | Life Technologies Corp | LUMINESCENT NANOPARTICLES AND THEIR PRODUCTION |
GB0119719D0 (en) | 2001-08-13 | 2001-10-03 | Solexa Ltd | DNA sequence analysis |
US20030064400A1 (en) * | 2001-08-24 | 2003-04-03 | Li-Cor, Inc. | Microfluidics system for single molecule DNA sequencing |
US7223541B2 (en) | 2001-08-29 | 2007-05-29 | Ge Healthcare Bio-Sciences Corp. | Terminal-phosphate-labeled nucleotides and methods of use |
US7033762B2 (en) | 2001-08-29 | 2006-04-25 | Amersham Biosciences Corp | Single nucleotide amplification and detection by polymerase |
US7052839B2 (en) | 2001-08-29 | 2006-05-30 | Amersham Biosciences Corp | Terminal-phosphate-labeled nucleotides and methods of use |
US7244566B2 (en) | 2001-08-29 | 2007-07-17 | Ge Healthcare Bio-Sciences Corp. | Analyte detection |
DE60235376D1 (en) | 2001-08-29 | 2010-04-01 | Ge Healthcare Bio Sciences | LABELED NUCLEOSIDE POLYPHOSPHATES |
US7214428B2 (en) | 2001-09-17 | 2007-05-08 | Invitrogen Corporation | Highly luminescent functionalized semiconductor nanocrystals for biological and physical applications |
DE60234382D1 (en) | 2001-09-17 | 2009-12-24 | Life Technologies Corp | NANO CRYSTALS |
US7205048B2 (en) * | 2001-09-17 | 2007-04-17 | Invitrogen Corporation | Functionalized fluorescent nanocrystal compositions and methods of making |
US8423294B2 (en) | 2001-09-18 | 2013-04-16 | Pathogenetix, Inc. | High resolution linear analysis of polymers |
US20030059822A1 (en) * | 2001-09-18 | 2003-03-27 | U.S. Genomics, Inc. | Differential tagging of polymers for high resolution linear analysis |
AU2003269813A1 (en) * | 2002-04-16 | 2003-12-31 | Princeton University | Gradient structures interfacing microfluidics and nanofluidics, methods for fabrication and uses thereof |
EP1497303A2 (en) | 2002-04-22 | 2005-01-19 | Joseph F. Lawler Jr. | Reagents for monitoring nuclei acid amplification and methods of using same |
US20030215864A1 (en) | 2002-04-23 | 2003-11-20 | U.S. Genomics, Inc. | Compositions and methods related to two-arm nucleic acid probes |
AU2003302463A1 (en) | 2002-05-09 | 2004-06-18 | U.S. Genomics, Inc. | Methods for analyzing a nucleic acid |
US20050257611A1 (en) | 2004-05-21 | 2005-11-24 | Halliburton Energy Services, Inc. | Methods and apparatus for measuring formation properties |
AU2003238796A1 (en) | 2002-05-28 | 2003-12-12 | U.S. Genomics, Inc. | Methods and apparati using single polymer analysis |
US7282330B2 (en) | 2002-05-28 | 2007-10-16 | U.S. Genomics, Inc. | Methods and apparati using single polymer analysis |
JP2005533256A (en) * | 2002-07-17 | 2005-11-04 | ユー.エス. ジェノミクス, インコーポレイテッド | Methods and compositions for analyzing polymers using chimeric tags |
WO2004020604A2 (en) | 2002-08-29 | 2004-03-11 | Amersham Biosciences Corp | Allele specific primer extension |
US7005518B2 (en) * | 2002-10-25 | 2006-02-28 | Li-Cor, Inc. | Phthalocyanine dyes |
JP2006520463A (en) | 2003-01-23 | 2006-09-07 | ユー.エス. ジェノミクス, インコーポレイテッド | Methods for analyzing polymer populations |
JP4896708B2 (en) | 2003-02-05 | 2012-03-14 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | Terminal phosphate-labeled nucleotides with novel linkers |
ES2355555T3 (en) | 2003-02-05 | 2011-03-28 | Ge Healthcare Bio-Sciences Corp. | AMPLIFICATION OF NUCLEIC ACIDS. |
WO2004092331A2 (en) * | 2003-04-08 | 2004-10-28 | Li-Cor, Inc. | Composition and method for nucleic acid sequencing |
JP2006522940A (en) * | 2003-04-10 | 2006-10-05 | ユー.エス. ジェノミクス, インコーポレイテッド | Manipulating polymers in microchannels |
US20050123944A1 (en) | 2003-08-01 | 2005-06-09 | U.S. Genomics, Inc. | Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions |
WO2005017205A2 (en) | 2003-08-04 | 2005-02-24 | U. S. Genomics, Inc. | Nucleic acid mapping using linear analysis |
WO2005022162A1 (en) * | 2003-08-21 | 2005-03-10 | U.S. Genomics, Inc. | Quantum dots and methods of use thereof |
KR101114748B1 (en) | 2003-09-23 | 2012-03-05 | 다우 글로벌 테크놀로지스 엘엘씨 | Self limiting catalyst composition and propylene polymerization process |
KR100558528B1 (en) * | 2003-09-25 | 2006-03-10 | 동부아남반도체 주식회사 | CMOS Image sensor and its fabricating method |
US20050142595A1 (en) | 2003-11-07 | 2005-06-30 | U.S. Genomics, Inc. | Intercalator FRET donors or acceptors |
WO2005047471A2 (en) | 2003-11-07 | 2005-05-26 | U.S. Genomics, Inc. | Improved fret efficiency methods |
US7169560B2 (en) * | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
US20050123974A1 (en) * | 2003-11-17 | 2005-06-09 | U.S. Genomics, Inc. | Methods and compositions relating to single reactive center reagents |
JP2007518107A (en) | 2004-01-13 | 2007-07-05 | ユー.エス. ジェノミクス, インコーポレイテッド | Detection and quantification of analytes in solution using polymers |
US20050196790A1 (en) | 2004-02-05 | 2005-09-08 | U.S. Genomics, Inc. | Methods for detection and quantitation of minimum length polymers |
WO2005080605A2 (en) * | 2004-02-19 | 2005-09-01 | Helicos Biosciences Corporation | Methods and kits for analyzing polynucleotide sequences |
US20060046258A1 (en) | 2004-02-27 | 2006-03-02 | Lapidus Stanley N | Applications of single molecule sequencing |
JP2007529758A (en) | 2004-03-19 | 2007-10-25 | ユー.エス. ジェノミクス, インコーポレイテッド | Compositions and methods for single molecule detection |
US20050239085A1 (en) | 2004-04-23 | 2005-10-27 | Buzby Philip R | Methods for nucleic acid sequence determination |
US7462452B2 (en) | 2004-04-30 | 2008-12-09 | Pacific Biosciences Of California, Inc. | Field-switch sequencing |
US20050260609A1 (en) | 2004-05-24 | 2005-11-24 | Lapidus Stanley N | Methods and devices for sequencing nucleic acids |
ATE507305T1 (en) | 2004-05-25 | 2011-05-15 | Helicos Biosciences Corp | METHOD FOR NUCLEIC ACID IMMOBILIZATION |
US20070117104A1 (en) | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
US20060024711A1 (en) | 2004-07-02 | 2006-02-02 | Helicos Biosciences Corporation | Methods for nucleic acid amplification and sequence determination |
US7276720B2 (en) | 2004-07-19 | 2007-10-02 | Helicos Biosciences Corporation | Apparatus and methods for analyzing samples |
US20060012793A1 (en) | 2004-07-19 | 2006-01-19 | Helicos Biosciences Corporation | Apparatus and methods for analyzing samples |
US20060024678A1 (en) * | 2004-07-28 | 2006-02-02 | Helicos Biosciences Corporation | Use of single-stranded nucleic acid binding proteins in sequencing |
WO2006036388A2 (en) | 2004-08-23 | 2006-04-06 | U.S. Genomics, Inc. | Systems and methods for detecting and analyzing polymers |
US7170050B2 (en) | 2004-09-17 | 2007-01-30 | Pacific Biosciences Of California, Inc. | Apparatus and methods for optical analysis of molecules |
AU2005296200B2 (en) * | 2004-09-17 | 2011-07-14 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
US20070048748A1 (en) | 2004-09-24 | 2007-03-01 | Li-Cor, Inc. | Mutant polymerases for sequencing and genotyping |
WO2007001438A2 (en) | 2004-10-29 | 2007-01-04 | Molecular Probes, Inc. | Functionalized fluorescent nanocrystals, and methods for their preparation and use |
WO2006055521A2 (en) | 2004-11-16 | 2006-05-26 | Helicos Biosciences Corporation | Tirf single molecule analysis and method of sequencing nucleic acids |
US7482120B2 (en) | 2005-01-28 | 2009-01-27 | Helicos Biosciences Corporation | Methods and compositions for improving fidelity in a nucleic acid synthesis reaction |
AU2006213692C1 (en) | 2005-02-09 | 2011-08-04 | Pacific Biosciences Of California, Inc. | Nucleotide compositions and uses thereof |
US7130041B2 (en) | 2005-03-02 | 2006-10-31 | Li-Cor, Inc. | On-chip spectral filtering using CCD array for imaging and spectroscopy |
US20060263790A1 (en) | 2005-05-20 | 2006-11-23 | Timothy Harris | Methods for improving fidelity in a nucleic acid synthesis reaction |
US8227621B2 (en) | 2005-06-30 | 2012-07-24 | Li-Cor, Inc. | Cyanine dyes and methods of use |
CA2616433A1 (en) * | 2005-07-28 | 2007-02-01 | Helicos Biosciences Corporation | Consecutive base single molecule sequencing |
US7666593B2 (en) * | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
US7509836B2 (en) * | 2005-09-01 | 2009-03-31 | Li-Cor, Inc. | Gas flux system chamber design and positioning method |
US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
US20070117102A1 (en) | 2005-11-22 | 2007-05-24 | Buzby Philip R | Nucleotide analogs |
EP1969153A2 (en) | 2005-11-28 | 2008-09-17 | Pacific Biosciences of California, Inc. | Uniform surfaces for hybrid material substrates and methods for making and using same |
US7998717B2 (en) | 2005-12-02 | 2011-08-16 | Pacific Biosciences Of California, Inc. | Mitigation of photodamage in analytical reactions |
US20070154921A1 (en) | 2005-12-16 | 2007-07-05 | Applera Corporation | Method and System for Phase-Locked Sequencing |
EP1989531B1 (en) | 2006-02-15 | 2022-01-05 | Li-Cor, Inc. | Fluorescence filtering system and method for molecular imaging |
US20080076189A1 (en) * | 2006-03-30 | 2008-03-27 | Visigen Biotechnologies, Inc. | Modified surfaces for the detection of biomolecules at the single molecule level |
US20090075252A1 (en) | 2006-04-14 | 2009-03-19 | Helicos Biosciences Corporation | Methods for increasing accuracy of nucleic acid sequencing |
US7282337B1 (en) | 2006-04-14 | 2007-10-16 | Helicos Biosciences Corporation | Methods for increasing accuracy of nucleic acid sequencing |
US20080241938A1 (en) | 2006-07-20 | 2008-10-02 | Visigen Biotechnologies, Inc. | Automated synthesis or sequencing apparatus and method for making and using same |
US20080241951A1 (en) | 2006-07-20 | 2008-10-02 | Visigen Biotechnologies, Inc. | Method and apparatus for moving stage detection of single molecular events |
US20080091005A1 (en) * | 2006-07-20 | 2008-04-17 | Visigen Biotechnologies, Inc. | Modified nucleotides, methods for making and using same |
US20080076123A1 (en) | 2006-09-27 | 2008-03-27 | Helicos Biosciences Corporation | Polymerase variants for DNA sequencing |
US8202972B2 (en) | 2007-01-10 | 2012-06-19 | General Electric Company | Isothermal DNA amplification |
WO2008137661A1 (en) | 2007-05-03 | 2008-11-13 | Helicos Biosciences Corporation | Methods and compositions for sequencing a nucleic acid |
US7678894B2 (en) | 2007-05-18 | 2010-03-16 | Helicos Biosciences Corporation | Nucleotide analogs |
US7767400B2 (en) | 2008-02-03 | 2010-08-03 | Helicos Biosciences Corporation | Paired-end reads in sequencing by synthesis |
EP2247741A4 (en) | 2008-02-03 | 2011-02-23 | Helicos Biosciences Corp | Paired-end reads in sequencing by synthesis |
US7790391B2 (en) | 2008-03-28 | 2010-09-07 | Helicos Biosciences Corporation | Methods of equalizing representation levels of nucleic acid targets |
WO2010051434A1 (en) | 2008-10-31 | 2010-05-06 | Helicos Biosciences Corporation | Polymerases and methods of use thereof |
-
2001
- 2001-12-03 AU AU2002227156A patent/AU2002227156A1/en not_active Abandoned
- 2001-12-03 US US10/007,621 patent/US7211414B2/en not_active Expired - Fee Related
- 2001-12-03 WO PCT/US2001/045819 patent/WO2002044425A2/en not_active Application Discontinuation
- 2001-12-03 EP EP01996079A patent/EP1354064A2/en not_active Withdrawn
-
2006
- 2006-12-29 US US11/648,108 patent/US20070172819A1/en not_active Abandoned
- 2006-12-29 US US11/648,114 patent/US20070172860A1/en not_active Abandoned
- 2006-12-29 US US11/648,721 patent/US8314216B2/en active Active
- 2006-12-29 US US11/648,723 patent/US20070172869A1/en not_active Abandoned
- 2006-12-29 US US11/648,107 patent/US20070172859A1/en not_active Abandoned
-
2009
- 2009-01-12 US US12/352,353 patent/US20100216122A1/en not_active Abandoned
-
2011
- 2011-01-06 US US12/985,421 patent/US20110184163A1/en not_active Abandoned
-
2012
- 2012-10-04 US US13/644,469 patent/US8648179B2/en not_active Expired - Lifetime
-
2013
- 2013-12-20 US US14/137,199 patent/US9243284B2/en not_active Expired - Fee Related
-
2015
- 2015-12-21 US US14/977,167 patent/US9845500B2/en not_active Expired - Lifetime
-
2017
- 2017-11-10 US US15/809,558 patent/US20180100191A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US8648179B2 (en) | 2014-02-11 |
US20180100191A1 (en) | 2018-04-12 |
US7211414B2 (en) | 2007-05-01 |
EP1354064A2 (en) | 2003-10-22 |
US20070172859A1 (en) | 2007-07-26 |
WO2002044425A3 (en) | 2003-08-21 |
US20100255464A1 (en) | 2010-10-07 |
US20110184163A1 (en) | 2011-07-28 |
US20160244822A1 (en) | 2016-08-25 |
US20070172860A1 (en) | 2007-07-26 |
US20100216122A1 (en) | 2010-08-26 |
US9845500B2 (en) | 2017-12-19 |
US20140127781A1 (en) | 2014-05-08 |
US20030134807A1 (en) | 2003-07-17 |
US20070172819A1 (en) | 2007-07-26 |
US20130102050A1 (en) | 2013-04-25 |
US20070172869A1 (en) | 2007-07-26 |
US9243284B2 (en) | 2016-01-26 |
WO2002044425A2 (en) | 2002-06-06 |
US8314216B2 (en) | 2012-11-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU2002227156A1 (en) | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity | |
WO2003060144A3 (en) | High fidelity dna polymerase compositions and uses therefor | |
AU2001255518A1 (en) | Compositions and methods for array-based nucleic acid hybridization | |
AU2002238418A1 (en) | Use of DNA repair enzymes as MMP-1 inhibitors | |
AU2001280968A1 (en) | Compositions and methods for directed gene assembly | |
AU2001276048A1 (en) | Rapid-melt semi-solid compositions, methods of making same and methods of using same | |
AU2001279284A1 (en) | Rapid-melt semi-solid compositions, methods of making same and methods of using same | |
AU2001253255A1 (en) | Compositions and methods for inhibiting gene expression | |
AU2001273548A1 (en) | Photoselection of nucleic acid ligands | |
AU2002325538A1 (en) | Nucleic acid amplification methods | |
AU2001278783A1 (en) | Method of amplifying nucleic acid | |
AU2625501A (en) | Compositions and methods for gene silencing | |
AU2001271720A1 (en) | Methods and compositions for determining gene function | |
AU2003270310A1 (en) | Compositions and methods for synthesizing nucleic acids | |
AU2599800A (en) | Methods and compositions for isolation of nucleic acid molecules | |
AU2001234455A1 (en) | Informative nucleic acid arrays and methods for making same | |
AU2839401A (en) | Enzymes and genes used for producing vanillin | |
AU2001245987A1 (en) | Compositions and methods for gene therapy | |
EP2110432A3 (en) | High fidelity DNA polymerase compositions and uses therefor | |
AU2001250428A1 (en) | Genes of the 1-desoxy-d-xylulose biosynthesis path | |
AU6094200A (en) | Nor gene compositions and methods for use thereof | |
AU2002245133A1 (en) | Methods of producing polyketide synthase mutants and compositions and uses thereof | |
AU2002243235A1 (en) | Novel nucleic acids, methods and transformed cells for the modulation of gibberellin production | |
AU2001274815A1 (en) | Compositions and methods for inducing activation of dendritic cells | |
AU2001260313A1 (en) | Compositions and methods for nucleic acid analyses |