Author: Reed Liu This is my first single cell practice. All the PDFs can be directly read!
I will share my learning process of 10X and Smart-seq2 to fans of Bioinfoplanet(公众号：生信星球).
- cellranger count操作
- 校正批次效应
- seurat V3操作（以PBMC 2700为例）
- seurat组合10X数据
- monocle2使用
- monocle3使用
- 用几个R包寻找HVGs
- Bioconductor-scRNA之基础操作
- mouce_c1下游分析全套
- 改造Seurat的DoHeatmap函数 【配套说明：如何改造Seurat包的DoHeatmap函数？】
- 改造inferCNV的热图【配套说明：如何改造inferCNV的热图？，来吧，自定义你的热图】
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Getting started with Cell Ranger by Dave TANG
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scRNASeqPipeline by tallulandrews
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singleCellSeq on GitHub
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ECCB 2018 Single Cell Tutorial of SIB course teaching
2016_Classification_of_low_quality_cells_from_single-cell_RNA-seq_data.pdf
- Single-cell RNA-sequencing: The future of genome biology is now, 2017 RNA Biol. [Recommend] 综述