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is it the 'clipped off poly-A tail' and 'clipped off poly-A tail' right? #422

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Bianzh1024 opened this issue Jan 8, 2024 · 1 comment

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@Bianzh1024
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I download the transcriptome fasta from uscs, https://hgdownload.cse.ucsc.edu/goldenpath/mm9/bigZips/mrna.fa.gz . when I used kallisto index to bulid the index for kallisto, that had two warning:

[build] loading fasta file mrna.fa.gz
[build] k-mer length: 31
[build] warning: clipped off poly-A tail (longer than 10)
from 32479 target sequences
[build] warning: replaced 196300 non-ACGUT characters in the input sequence
with pseudorandom nucleotides
[build] counting k-mers ... done.
[build] building target de Bruijn graph ... done
[build] creating equivalence classes ... done
[build] target de Bruijn graph has 3001158 contigs and contains 263628489 k-mers

And it succeed. Why it showed the clipped and non-ACGUT ? The index is right?
I hope you can give me some advice. Thanks! @pmelsted

1111

@Yenaled
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Yenaled commented Jan 10, 2024

Yes it’s fine

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